LOCUS       DAYYHB010000001        83936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000001 DAYYHB010000000
VERSION     DAYYHB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.001"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1100
                     /locus_tag="ACHA6B_000001"
     CDS             99..1100
                     /locus_tag="ACHA6B_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8947613.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1119..2138
                     /locus_tag="ACHA6B_000002"
     CDS             1119..2138
                     /locus_tag="ACHA6B_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8947614.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2240..2956)
                     /locus_tag="ACHA6B_000003"
     CDS             complement(2240..2956)
                     /locus_tag="ACHA6B_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8947615.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2925..3710)
                     /locus_tag="ACHA6B_000004"
     CDS             complement(2925..3710)
                     /locus_tag="ACHA6B_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8947616.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3701..4450)
                     /locus_tag="ACHA6B_000005"
     CDS             complement(3701..4450)
                     /locus_tag="ACHA6B_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8947617.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4748..5272
                     /locus_tag="ACHA6B_000006"
     CDS             4748..5272
                     /locus_tag="ACHA6B_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8947618.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5288..6787
                     /locus_tag="ACHA6B_000007"
     CDS             5288..6787
                     /locus_tag="ACHA6B_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8947619.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6875..7477)
                     /locus_tag="ACHA6B_000008"
     CDS             complement(6875..7477)
                     /locus_tag="ACHA6B_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8947620.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7581..8537
                     /locus_tag="ACHA6B_000009"
     CDS             7581..8537
                     /locus_tag="ACHA6B_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8947621.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8842..9798)
                     /locus_tag="ACHA6B_000010"
     CDS             complement(8842..9798)
                     /locus_tag="ACHA6B_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8947622.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9972..10532
                     /locus_tag="ACHA6B_000011"
     CDS             9972..10532
                     /locus_tag="ACHA6B_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947623.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10525..11349
                     /locus_tag="ACHA6B_000012"
     CDS             10525..11349
                     /locus_tag="ACHA6B_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8947624.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11359..11976
                     /locus_tag="ACHA6B_000013"
     CDS             11359..11976
                     /locus_tag="ACHA6B_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8947625.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11994..12677
                     /locus_tag="ACHA6B_000014"
     CDS             11994..12677
                     /locus_tag="ACHA6B_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8947626.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13360..14643
                     /locus_tag="ACHA6B_000015"
     CDS             13360..14643
                     /locus_tag="ACHA6B_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8947627.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVNKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14659..15546
                     /locus_tag="ACHA6B_000016"
     CDS             14659..15546
                     /locus_tag="ACHA6B_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947628.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15563..16399
                     /locus_tag="ACHA6B_000017"
     CDS             15563..16399
                     /locus_tag="ACHA6B_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947629.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16402..17025
                     /locus_tag="ACHA6B_000018"
     CDS             16402..17025
                     /locus_tag="ACHA6B_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947630.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17022..18314
                     /locus_tag="ACHA6B_000019"
     CDS             17022..18314
                     /locus_tag="ACHA6B_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8947631.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18673..18770
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18886..19467
                     /locus_tag="ACHA6B_000020"
     CDS             18886..19467
                     /locus_tag="ACHA6B_000020"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8947632.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19470..20777
                     /locus_tag="ACHA6B_000021"
     CDS             19470..20777
                     /locus_tag="ACHA6B_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8947633.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMILISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21193..21681
                     /gene="purE"
                     /locus_tag="ACHA6B_000022"
     CDS             21193..21681
                     /gene="purE"
                     /locus_tag="ACHA6B_000022"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8947634.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21674..22798
                     /gene="purK"
                     /locus_tag="ACHA6B_000023"
     CDS             21674..22798
                     /gene="purK"
                     /locus_tag="ACHA6B_000023"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8947635.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22811..24106
                     /gene="purB"
                     /locus_tag="ACHA6B_000024"
     CDS             22811..24106
                     /gene="purB"
                     /locus_tag="ACHA6B_000024"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8947636.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24286..24663)
                     /locus_tag="ACHA6B_000025"
     CDS             complement(24286..24663)
                     /locus_tag="ACHA6B_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947637.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24919..25647
                     /gene="purC"
                     /locus_tag="ACHA6B_000026"
     CDS             24919..25647
                     /gene="purC"
                     /locus_tag="ACHA6B_000026"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8947638.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25647..25901
                     /gene="purS"
                     /locus_tag="ACHA6B_000027"
     CDS             25647..25901
                     /gene="purS"
                     /locus_tag="ACHA6B_000027"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8947639.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25903..26574
                     /gene="purQ"
                     /locus_tag="ACHA6B_000028"
     CDS             25903..26574
                     /gene="purQ"
                     /locus_tag="ACHA6B_000028"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8947640.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26575..28797
                     /gene="purL"
                     /locus_tag="ACHA6B_000029"
     CDS             26575..28797
                     /gene="purL"
                     /locus_tag="ACHA6B_000029"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8947641.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28782..30221
                     /gene="purF"
                     /locus_tag="ACHA6B_000030"
     CDS             28782..30221
                     /gene="purF"
                     /locus_tag="ACHA6B_000030"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8947642.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30244..31296
                     /gene="purM"
                     /locus_tag="ACHA6B_000031"
     CDS             30244..31296
                     /gene="purM"
                     /locus_tag="ACHA6B_000031"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8947643.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31293..31871
                     /gene="purN"
                     /locus_tag="ACHA6B_000032"
     CDS             31293..31871
                     /gene="purN"
                     /locus_tag="ACHA6B_000032"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8947644.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31868..33403
                     /gene="purH"
                     /locus_tag="ACHA6B_000033"
     CDS             31868..33403
                     /gene="purH"
                     /locus_tag="ACHA6B_000033"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8947645.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33419..34693
                     /gene="purD"
                     /locus_tag="ACHA6B_000034"
     CDS             33419..34693
                     /gene="purD"
                     /locus_tag="ACHA6B_000034"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8947646.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34815..35729
                     /locus_tag="ACHA6B_000035"
     CDS             34815..35729
                     /locus_tag="ACHA6B_000035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8947647.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35862..37427)
                     /locus_tag="ACHA6B_000036"
     CDS             complement(35862..37427)
                     /locus_tag="ACHA6B_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8947648.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37420..38871)
                     /locus_tag="ACHA6B_000037"
     CDS             complement(37420..38871)
                     /locus_tag="ACHA6B_000037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8947649.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38885..39901)
                     /locus_tag="ACHA6B_000038"
     CDS             complement(38885..39901)
                     /locus_tag="ACHA6B_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8947650.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40121..41209
                     /gene="chvE"
                     /locus_tag="ACHA6B_000039"
     CDS             40121..41209
                     /gene="chvE"
                     /locus_tag="ACHA6B_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8947651.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41263..42789
                     /gene="mmsA"
                     /locus_tag="ACHA6B_000040"
     CDS             41263..42789
                     /gene="mmsA"
                     /locus_tag="ACHA6B_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8947652.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42791..43996
                     /gene="mmsB"
                     /locus_tag="ACHA6B_000041"
     CDS             42791..43996
                     /gene="mmsB"
                     /locus_tag="ACHA6B_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8947653.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44253..45230
                     /gene="guaC"
                     /locus_tag="ACHA6B_000042"
     CDS             44253..45230
                     /gene="guaC"
                     /locus_tag="ACHA6B_000042"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8947654.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45494..46918
                     /locus_tag="ACHA6B_000043"
     CDS             45494..46918
                     /locus_tag="ACHA6B_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8947655.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(46960..47217)
                     /locus_tag="ACHA6B_000044"
     CDS             complement(46960..47217)
                     /locus_tag="ACHA6B_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947656.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            47379..48488
                     /gene="pdhA"
                     /locus_tag="ACHA6B_000045"
     CDS             47379..48488
                     /gene="pdhA"
                     /locus_tag="ACHA6B_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8947657.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48491..49468
                     /locus_tag="ACHA6B_000046"
     CDS             48491..49468
                     /locus_tag="ACHA6B_000046"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8947658.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49493..51136
                     /locus_tag="ACHA6B_000047"
     CDS             49493..51136
                     /locus_tag="ACHA6B_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8947659.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51144..52550
                     /gene="lpdA"
                     /locus_tag="ACHA6B_000048"
     CDS             51144..52550
                     /gene="lpdA"
                     /locus_tag="ACHA6B_000048"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8947660.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52712..52987
                     /locus_tag="ACHA6B_000049"
     CDS             52712..52987
                     /locus_tag="ACHA6B_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8947661.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52993..53760
                     /locus_tag="ACHA6B_000050"
     CDS             52993..53760
                     /locus_tag="ACHA6B_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8947662.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53928..55757
                     /gene="typA"
                     /locus_tag="ACHA6B_000051"
     CDS             53928..55757
                     /gene="typA"
                     /locus_tag="ACHA6B_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8947663.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56018..57790
                     /gene="recQ"
                     /locus_tag="ACHA6B_000052"
     CDS             56018..57790
                     /gene="recQ"
                     /locus_tag="ACHA6B_000052"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8947664.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57904..58206
                     /locus_tag="ACHA6B_000053"
     CDS             57904..58206
                     /locus_tag="ACHA6B_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8947665.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58209..59372
                     /locus_tag="ACHA6B_000054"
     CDS             58209..59372
                     /locus_tag="ACHA6B_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8947666.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKNFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59398..62826
                     /locus_tag="ACHA6B_000055"
     CDS             59398..62826
                     /locus_tag="ACHA6B_000055"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8947667.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62847..63971
                     /locus_tag="ACHA6B_000056"
     CDS             62847..63971
                     /locus_tag="ACHA6B_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8947668.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63952..64398
                     /locus_tag="ACHA6B_000057"
     CDS             63952..64398
                     /locus_tag="ACHA6B_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8947669.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRVVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64382..64720
                     /locus_tag="ACHA6B_000058"
     CDS             64382..64720
                     /locus_tag="ACHA6B_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8947670.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64839..65399
                     /gene="rsmD"
                     /locus_tag="ACHA6B_000059"
     CDS             64839..65399
                     /gene="rsmD"
                     /locus_tag="ACHA6B_000059"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8947671.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65396..65887
                     /gene="coaD"
                     /locus_tag="ACHA6B_000060"
     CDS             65396..65887
                     /gene="coaD"
                     /locus_tag="ACHA6B_000060"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8947672.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65877..66920
                     /locus_tag="ACHA6B_000061"
     CDS             65877..66920
                     /locus_tag="ACHA6B_000061"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8947673.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67079..67807
                     /locus_tag="ACHA6B_000062"
     CDS             67079..67807
                     /locus_tag="ACHA6B_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8947674.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67923..68168
                     /locus_tag="ACHA6B_000063"
     CDS             67923..68168
                     /locus_tag="ACHA6B_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8947675.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68165..68383
                     /locus_tag="ACHA6B_000064"
     CDS             68165..68383
                     /locus_tag="ACHA6B_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8947676.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68519..69058
                     /locus_tag="ACHA6B_000065"
     CDS             68519..69058
                     /locus_tag="ACHA6B_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8947677.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69051..71324
                     /locus_tag="ACHA6B_000066"
     CDS             69051..71324
                     /locus_tag="ACHA6B_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8947678.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71362..72408
                     /gene="holA"
                     /locus_tag="ACHA6B_000067"
     CDS             71362..72408
                     /gene="holA"
                     /locus_tag="ACHA6B_000067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8947679.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72497..72751)
                     /gene="rpsT"
                     /locus_tag="ACHA6B_000068"
     CDS             complement(72497..72751)
                     /gene="rpsT"
                     /locus_tag="ACHA6B_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8947680.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73151..73420)
                     /locus_tag="ACHA6B_000069"
     CDS             complement(73151..73420)
                     /locus_tag="ACHA6B_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947681.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73682..73810)
                     /locus_tag="ACHA6B_000070"
     CDS             complement(73682..73810)
                     /locus_tag="ACHA6B_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947682.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(73890..74345)
                     /locus_tag="ACHA6B_000071"
     CDS             complement(73890..74345)
                     /locus_tag="ACHA6B_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8947683.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74483..76411)
                     /locus_tag="ACHA6B_000072"
     CDS             complement(74483..76411)
                     /locus_tag="ACHA6B_000072"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8947684.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(76608..77441)
                     /locus_tag="ACHA6B_000073"
     CDS             complement(76608..77441)
                     /locus_tag="ACHA6B_000073"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8947685.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77633..78337)
                     /locus_tag="ACHA6B_000074"
     CDS             complement(77633..78337)
                     /locus_tag="ACHA6B_000074"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8947686.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78360..78986)
                     /locus_tag="ACHA6B_000075"
     CDS             complement(78360..78986)
                     /locus_tag="ACHA6B_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8947687.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79312..81687
                     /locus_tag="ACHA6B_000076"
     CDS             79312..81687
                     /locus_tag="ACHA6B_000076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8947688.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(81711..82202)
                     /locus_tag="ACHA6B_000077"
     CDS             complement(81711..82202)
                     /locus_tag="ACHA6B_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8947689.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82389..83309
                     /locus_tag="ACHA6B_000078"
     CDS             82389..83309
                     /locus_tag="ACHA6B_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8947690.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83463..83807
                     /locus_tag="ACHA6B_000079"
     CDS             83463..83807
                     /locus_tag="ACHA6B_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8947691.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        27208 a        14548 c        18062 g        24118 t
ORIGIN      
        1 ttttttattg tgaataaatg aacataatta ggaggagaaa gatgaactta tttgacattt
       61 ttcgatcgac tttgacagat atgtcgattg tcagtgcaat cacttcaaca gtttttatta
      121 tattattagg gtttgtttgt agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc
      181 tatcgaaagt cgtcttaaca gtggctttgc cagcactggc atttcgagct ttcatgcaag
      241 atatcaatac agaattactg atgcaaggaa tcaatgtctt gatttgggga ttagttattt
      301 atattattct tattctagtt tcaaaacctt ttttcttaag ctataaaggg gataagcaaa
      361 caacgttgcg tgtcctgacg atctttggtt ctacgacctt tttcggaacg cctatcgtca
      421 gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa
      481 tctttttgta ttcttatgga tacataaaaa tgagtggctt gaagatggaa gctaaaaata
      541 tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt tgccggcctt ttcatttggc
      601 tgttccaagg gtatctgcca caagtgtcag ttacaggtgc agaaggagag ataagccaag
      661 tagcgttttt gcgaattgac cagactgcac cgtggctatt tcagccgatg gattatttag
      721 ctggtttaag ttcaccattg gcgtggctag caatcggcgc aacattaggt gaagtaagct
      781 tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag tctagtcaaa gtggtatttg
      841 tacctgcatt gaatatcgtg attctagcag ttttgacggc aacgaatatt ctaccagtca
      901 attttgaagc actggcaaca atcgttatca tgatggctac accagctgca acagtggcag
      961 cagcctatgc tatcagtttt gatagggagg cacttttagc ctccaatgca tccttactgt
     1021 caactatttt tgctgtggta ctgacacctg tatggatcgt tgtattacaa atgattcatg
     1081 gcataggttt attttcataa gaaatggagg aagaaagtat gagaatcgca tgttacggtg
     1141 ttcgtccgaa cgagatagat ttttttgaaa aattgaatat ttaccattat gatctcagtc
     1201 tttatgaaga actgttgaca catgacaata tcgtgtcagc aaaggaccat gatgctgtat
     1261 tgttgagggg aaattgtata gcagatgaga ccaatcttgc aaaaatgcag gaatatggga
     1321 ttcgttatgt ttttacacgg actgtgggag tcaatcatat cgatctacaa gcggcagctg
     1381 attttggtat gacagtagcc cgtgtaccat cgtattcgcc aaatgcgata gcagagttat
     1441 ctttgacttt tgccatgatg cttttgagaa atactgcgta tacgacgatc cgaacatctt
     1501 tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag
     1561 ggatcatagg aacaggcagg atagggttga cggaagctgt tttgtttaag ggactaggag
     1621 ccaaagtgat cggctacgat attcatcaga cagaagctgc taaaaaagta ttgacgttcc
     1681 actcgcttga agagttatta gccaaaagcg atattgtcag tattcatgta cctcatatcc
     1741 caggtgaaaa cgatcaaatg atcaatgaag gatttttagc tcaaatgaaa aaaggtagca
     1801 tcttgattaa tacagcccgt ggagagcttc aagataatga agctatttta agagctttga
     1861 aaagcaatca tttagctggt tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt
     1921 tccgatcctt caagccatgg gagacgattc cagaccctgc tatccagcaa ttagtagaaa
     1981 tgtaccctag agttcttgta accccgcatg taggttcaaa tacagatgag gcattatcaa
     2041 atatgatttc gacaagtttc gagaactttc gtgaaataat agaaactgga aaaacgaaaa
     2101 atgaggtatc tcttcctaaa gcccgtcaat taagataaga aaacatggat attcgtcaat
     2161 aaaatgttta aaaatagtcc aagtaaacag ttgttacttc tgtatacttg gactattttg
     2221 tatgggataa ttcgatgact caacagtaat ctgtgagctt tttccgccca ttctttgtca
     2281 attcataaca ggttagcggc cggccgattt ccaaatattt gattctttcg ttcaatatat
     2341 cttgttctac taaaaaattc aaatatttct tgacggatat acgtgacagt gagctttttt
     2401 gagccagttc ttttgttgaa aaaatagtat cttcttttag aataaatgca cttatttttt
     2461 ttaaagtcag ttttgacaga cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg
     2521 gagtggtgtg gttgaatact tgatcgatca atttctgatc ggctttttca gtcgtttgga
     2581 ttgtctgaga gaaagcaaga tacttttcga tcgccagctg aaaacgttgc aaagtgaacg
     2641 gtttgatcaa ataatctaag gcaccgtagc ggatcacatt ctttatggtt tcgatatcat
     2701 ctgcagcagt gatcaaaatc actgggatat catttccttg ctgtctaagc caagataaaa
     2761 aatggatacc actttcgcca ggcatgtata catcaagcag cagcagatct gcgttttttt
     2821 gtctgaaata tttttttcct tcttctacat ttctgaaact ttcgattacg ttcatattag
     2881 ggatgctttc cacaaaagta cgattcaatt ctgcaaccat tggatcatct tcgattatca
     2941 ggacatttgt catcattttg atcttccttt tttggtaatt ctagatagac gattgtaccg
     3001 ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg
     3061 agattcaatc caaacccacg attgattccc ttcgtcgaaa aacctagcgt aaatattttt
     3121 tctgagattt cttctgatat cccgacaccg tcatcgcaga cggtcacgat gaatacttca
     3181 gcttctgtgt catagtttaa atgaatccgg acctgtccgt ttctttcatg tttttgtagc
     3241 acggcttctt ttgcattatc aagtaagttc cctaacactt gcaaaagtga atggatggag
     3301 tcatccatct ccattttagg gataaaagaa tcgctttcta acagcatttc aacagattgt
     3361 tctttggctt cgtttatttt gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga
     3421 cttgagactt ggccaatctc ttttttataa ttttgattta gctgctggat aaaatgggtg
     3481 acttcttcat acttcttcaa atcaatcaaa ccagaaatga catgaagttt attcatgaat
     3541 tcatgggttt gagctctcat ggagtttata tattgtttcg tccccccaag ctcctccatt
     3601 aatttcatta cttctgatcg atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg
     3661 ctaaaaagag gtgtaacgct agaaatagtt tctatcccat ttaaatacat ggattgagtc
     3721 cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa cgtcatatag attatcactg
     3781 atttttttcc tacttcgaaa tccaatagct gctctgtttg gtagaaaatg ttttttgcag
     3841 gtgtattgat caagaggata gttttatcag aagagatggc taagattcct tcatctactt
     3901 cattttcgat aatcgttttt tcacttaact gttctgctat ttcttgtggt tcaagtccaa
     3961 gcagtacttt tttcattttt tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc
     4021 caataatcaa tgaaaaaaca atcatttttt gtccatcttt gacttgattg tcaatcgtgt
     4081 tgtttgttaa gccaacacaa actgcaccaa taatctcttg atcataataa atagggacga
     4141 aatagcggaa gccgaaccct aacattcctt ctttttcaga gaaaaattct tgtcctccaa
     4201 tcactttttt cacatcttca tgacttgaga aaggaagacc gatgtaactt gggtctgcgt
     4261 gagaatatcg aatgccttca gggtccatga caacgataaa atctaaattc gatgcaatca
     4321 tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc accagtctgc agcttttttt
     4381 ggatgagttt atcattggca acgattttag caatgctaga gattttatct gacacactag
     4441 atcggttcat cgaaagaata aaattcctga tcaatatggc agaaacaatc aaagaaacga
     4501 atgtggagaa acaaacgaca aggatgatcg tgttctggag ctttaatggt tttttcttaa
     4561 aaaaaggaaa cataggctac ctcgaaagaa tttattgtga aaattttaac atgaaaacaa
     4621 aaatttaaca aattgtcaac gataaacaca accttcctat aaaaaacacc actgagtcgg
     4681 taaaagaagc tatacagaat tttcaaacat tggtatgata gacgtagaaa tggaaggagg
     4741 cgtgtatatg atagggggaa tcgcacttga gacgatttat tggtatacat tgctgatttg
     4801 tgcaggattg gcagtcctat tgattatttt tggagatgta ttcgattttg atggcccgct
     4861 tgatccgatg cttattatcc cttggttaac tttcacaagc ttatttggtt ttctaggtga
     4921 gcgtttaaca gatcaatcat ctttattttt atttattgct agtggaggca tatcgatagt
     4981 gttggtattt ttgcttaatt tctatatatt gatgccgatg aaacatgccg aatcaacgat
     5041 gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt gtgatcacac cgatccctgt
     5101 acgaggtatg ggagaaattc agttgaaaag tgttaccggt tccattagtc gtccagcttg
     5161 tttttacagc ccccaagaac agacagctga gcggggaacg aaagtattga tcattgagat
     5221 caaggaaaga atctgttatg tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt
     5281 gtgttaaatg gaaataccag ttgtttttat cgcgatcgca atcgcagctt tgattttact
     5341 gatgttgctg atcgtctttg tagcgaagta ccagacagca aaaccggatg aagcgttgat
     5401 catcagtgga agttatttag gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat
     5461 caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat
     5521 cagtctatta tcgagtaaat tggacgtctc cacccctgaa gtgtatacgg aacaaggcgt
     5581 accggtgatg tgtgatggta cttcgatcat caaaatcggt tcatctgtag aagagattgc
     5641 tacagcagca gaacagtttt taggcaagac aagagaagaa ctggaaaatg aagcaagaga
     5701 agtattagaa ggacacttgc gctcgattct tggttctatg actgtagaag aaatctatca
     5761 aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat
     5821 ggggttgatc atcgtttctt tcactatcaa agaggtcaga gacaaaaacg gttatcttga
     5881 ctctttaggg aaaccaagaa tcgcccaagt caaaagagat gcagatatag cagaagcgga
     5941 agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga
     6001 attacagcga caaacagaga tcgcagaatc attaaaagaa aaagaactga aattagcaac
     6061 ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac caagcctaca atttagaaag
     6121 tgcccgagca caacaacaag taatcgaaca ggaaatgcag attaaaatcg tagaacgtca
     6181 aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga
     6241 agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa caagaagcac aagctcaaaa
     6301 agtcaaagaa gtgactgaag ccgaagcaga acgattccgt gtggaagcac tggcagaagc
     6361 ggaagccaat aagacacgtt tagctggaca agcagaagcc gaagcagcac tagcaaaagg
     6421 aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc
     6481 agctgtcttg agtatggtta tcgatatgct accgcaattg atgcgcgaag cagcacagcc
     6541 gctaggaaat atcgaaaaaa tctctgttgt cgatacagga agcagttcag gagaaaccag
     6601 tggagcgaac cgagtgacaa attatgccac aaatttgctt tcttctactc aagaaacact
     6661 aaaagcgaca acaggacttg acttgaagga aatcgtagag aatttgtcga cacgtgggac
     6721 tactagccaa gtaaatatgt atgaagcgcc aaaagaggca gaacaaaact cgatgaagga
     6781 agaataaagt agtacacttc accgttcaat caaaaatgga ggaagacttt ttaaagttct
     6841 tctctccatt tttttatttt attgcaaaag aaaattagtc ctcgctattt ttcttttgtt
     6901 tttggatctt agcttctatc tcttgtaaat ttgttcttag caatcgttct ttcttttgga
     6961 gttcgaccaa atcttctgat agttcttcct tcttttctgt ttcaggagga cgaagaaaga
     7021 aattgatacc gattgcaatg aatgtcccta taaaagtatc tagtacacgt tggatagcat
     7081 acaaagcgga ttcgccttga gggatgctca acgcaatcag caacaacgta gctattgcgg
     7141 aaatgatacc ggaattattg ttaataccat ctgacacgac gatcacaata ataactagaa
     7201 taggaagcaa gatcaattct accaaaaaat cattgtgaaa atagcttttg acaaaaaaat
     7261 aaatgatagc taatgtacca cctaatgtat tgccgatgat cctagacttt ccaaaagaaa
     7321 cggttgtagt caagtcctga cgcaaagaaa aaacagcaga taatgcagca atcatcggga
     7381 ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa aacagctaag gcacttttga
     7441 tcgtacgcat acctagtcga aaacggccaa attgcataga gattctcctt tttggtcttt
     7501 ttactatcat acctaaactt ttgtctgata gctagttttt cattattcaa gtataattaa
     7561 aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg
     7621 tcttcttttc gatggtcaca gcgggtagct tatcgctgtt gtattggcca gctgcaggga
     7681 tcattgcatt attaagtgta ggaaatacca agcgctctac attgcgtacg ggcatgaatc
     7741 gaatcgcagc ctttatatta gctacggcaa cagcactgtt cagttttttt gctgttgggt
     7801 atactatttt tggcttcagt ctttttttat tcctgttcat cccgcttgcc tctcgcttca
     7861 aattaaccga agggatagta gtaaattcgg ttttggtcac tcattattta gtggaggaaa
     7921 acatgtcttg gcagctgatc ggcaatgaag cgggattaat ggcgattgga ttagtatttg
     7981 ctttgcttgc aaatgtttac atgccagatc gtacgaagca gctgaaggaa aaccaaatac
     8041 agatcgaaaa agaatttcga aatattttga gacaaatggc agaatttctt ttatcagaaa
     8101 ataaagaaga tgtgcagatc aaatgcgaac atttactgac attcattcgc gaaagccaag
     8161 aagatgccag agaacatcaa gaaaactact ggctcagaca gccgctatac tacgaaactt
     8221 acttctctat gagaagagca caggccaatg tgattaaaga catgctggaa aatctagaga
     8281 gaatccagca accagcttat tatggaaaac atatctatgg tttattgatt tatactgcag
     8341 aaacattttc agagtcaaat gatggcagac aaattttaac acgtattgaa gaagtttatg
     8401 tattgtatcg gcaaatgcct ctgccgacta gtcgccctga attcgaggat cgagcagaat
     8461 tgttccagtt tttgcagtct tttaaaagtt ttattgaaat caaggcagaa ttctcccaac
     8521 aaaaaatcca aaaatagagg ttgtgaattc agctaatatt caggtcaaaa agctgatttc
     8581 acaacctctt ctttaaacgg tgttctcctg tcaactcctc ggtcacaagt cacaatattc
     8641 aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcggagct gaacggtagg
     8701 ctcacaacct ctttcttaaa cggtgttcaa aagctttcct ctgcggtaat aagtccagcc
     8761 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
     8821 ctttttcaca accttttaaa attacatatt tttccataga acttgcattt gatccaagat
     8881 ttcgatgaca tcttttgtca agaagagaga gcgattgggc aagtcacctt caatcatgtg
     8941 taccatattc tttatttcgt aattcattgc ttcagatgta ttcccgcttt cgatgaattc
     9001 ctttgtacca tctgtaaata agatctccgc acgatcagca cgcgggtaat cagcaatcgt
     9061 cacataaccg ttctcaaagg caacgatacc gacttttggc atttttgcct gaaaagtcaa
     9121 gctgacagca gcaagttcat tttctttgtt acgcaagata gtgacggatt gttcgtccac
     9181 gcctgtttca aaaggaacca ttgtagaggc aactacttca ggttgagagc tcaaaaagaa
     9241 acgagcgaaa gatacagcat acgtaccgat atccaacaga gctcctccag ctaattctgg
     9301 attgaagaac cggtttttag ggtctggttc tttgtaactg ccgaaaggtg cttggatcat
     9361 ttttaaagcc cccagttttc ctgtatccat tattgagcgt aactgctgat aaagtggcat
     9421 gttgaagatt gtcatcgctt ctgctaagat caaattcttt tcttctgcta agcgcatagc
     9481 gtcagccagt tcttttttat tcatcgtgat ggctttttca catagaacgt gtttgcctgc
     9541 ttccaatgct gccaagatat ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat
     9601 gtcgatttca gcatcgtcta acatttcttg atagcttccg taagcttttg gaatattgta
     9661 acggtgagcg aagttttccg ctttttctga agttctggaa gcaactgctg ttagttcgct
     9721 cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg gtaccaagac caacgattcc
     9781 ccattgatat tttttcatct gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt
     9841 ctattattat aacaaaaatc acgaagaata aagaggagag gacttgaaac attaaaaaaa
     9901 atgaaagcag cgatttaaga ggtgcttttt gatcaaaact taagtagaat agttaaaagg
     9961 aggggataaa cgtgcatcaa gtatatattg atcttattat gagtgtatta ttagaacagt
    10021 ttgaaactga aaaaaatttt ttagatgaat acttgctgat cagtccagaa caatggcaaa
    10081 aatggaaaga tggcgagaaa aatctgccat ctgaagcgat gcagaaagta aaaaatcttt
    10141 tcactgatta tgaatggatg ctaacgcaaa agattttgcg tcaaacgatt atttttcccg
    10201 aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat
    10261 ggcttagcag caaagcagca aaagtcgagc tgatcccttt gaaaaataat cagcaggctg
    10321 gtagatacct tgacttgaaa gtaagtattc aatatgatga atggggctat gatgatattt
    10381 tgaattttcg tctgccagca gatgttcaaa aacagatcga aggagaaaaa atcgaacttc
    10441 tcgattgggt gaatgaaaat ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg
    10501 aagaacaacg aaaggatttt tcaagtgaat aaatttttag gatttggagc agttgtgatt
    10561 atgatcgcag caatgggcta tgttattttt agcgatgatg attcgcatgt tcaagccggc
    10621 aatcaagcag tcgaacgaac aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa
    10681 acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc gtacgtccaa acaattgcca
    10741 gatgttaaaa cagacgattg gaagctagtt ttggttggtc cagatcacaa attagaaaaa
    10801 gaaattgatg agtctactca attggcatca ttaagtaacg gatacttgat cgacaaacgc
    10861 attgctccag aatatgaagc atttgccaag gctgctgaag cagctggatt tccattagtg
    10921 atggtctcag cttaccggtc tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa
    10981 gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga caaagattac taaagaaacg
    11041 attacggagc caggatacag cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa
    11101 aactggtaca acagttatcc ttctactgta ttggatgcaa gttacggaga tcagcctgga
    11161 gctaaatgga tcgcagataa tgctgcgcag tatggattta tcgttcgtta tccaaaagga
    11221 cacgaagata taactaagat cacatatgaa ccttggcact tacgttatgt cggtaaagaa
    11281 aacgctgagt atatcaccaa acaccaattg acgctagaag aatatctgaa tgaattaaat
    11341 gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc
    11401 cgttggccag cagtggttgc cggtgtagca gtgatactct tagcctttgt tttttccttg
    11461 aagaatctgt cttctccttt tactgataat cagcaagaaa atgaagcgca atcccatcag
    11521 caatttattg aacggcttgt tccgcatgcc caagaactac aagatggtta tggagtgctt
    11581 cctagtatca ttttaggaca agctatatta gaatcaaatt ggggaaaaag taccttggct
    11641 agcaaataca ataatctttt cgggatcaag gcttatgggg atcagaagaa agtaagcctg
    11701 gaaacaaagg aatttgttaa cgaagaatgg ataactatcc aaggagactt caaagtgtat
    11761 gattcatggg aacagtccat ggatgaccat acacaactgt tcgtccaagg agtagattgg
    11821 aatcctgcgc tgtatgaaaa agtgattacg gccaccaact atcaggaagc cgcacaagca
    11881 ttgcaagatg caggttatgc gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc
    11941 gagacgtatc aattgaatca gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag
    12001 aacaatttac gccagaaatc atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa
    12061 tcattttaga agcaagcggg atcaagatat ttggaaaatt gatccgttcg atcatcttta
    12121 ctacggatat tgcgatcata cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt
    12181 ttacacctca tccagcgatt actaaaagca ttattgaggc agctgacatt ccagttttct
    12241 ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc ctacatgagc atgttcgcag
    12301 aagcgcaagg ttcgttaggg gtcgttttga acggtcctac gcctttagag acagtaaaag
    12361 aagtttgtcg agtagttgat attccagtta tcagtacggt aacgtcgaaa tatacagaaa
    12421 tagatgaaaa gttagcagct ggtatttcga ttatcaatat cagtgcaggt aaagaaactg
    12481 cagaagcagt acgccatttt cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg
    12541 gtccaacgga agaaagtatt gctgagacca ttcaagcagg agcaaatgcc atcacatata
    12601 cgccaccaag tagcggagag ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga
    12661 aaaagaatca tgaataacag gtgagaatca aaagacgagg aagagcagat agtggctctt
    12721 cctcgtcttt tgtactccag tactatttgt tcagaattca aaaaaaagag aaattttttt
    12781 gtttttttac ctgtaatgta cataagaaga gttaaaacct tttgttatta ttagcattct
    12841 aacaaaaaaa tgtcttgatt tattgaagcg ctttctatag aatgaaaaac gaataaaaac
    12901 taaaaaaggc acccgacaat ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg
    12961 gaactgacca atcaagttgc catagtcaat gcttgtgcat cgacgtgtat attgtaccta
    13021 atttcataca gaattactag ttatttttct gttttttagg gaagagcgca cacgaagaag
    13081 catgcttatt tagctatgtc aaggatattg ggcagttgtc tgatatcctt ttttctttgc
    13141 gtaattatcc tcccatcatt gaagcaagaa gtgtttttag cgatgaaaga agaatcagca
    13201 ctatctaatt tttcttgaaa gtattgagtt gttcatccta ggcagaacga taaggcaagt
    13261 cttggttttt attccccttg aaaaagatcg tttaataaat atgttctgta taaaaataca
    13321 gtatattgaa aaataacaat ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc
    13381 atcttagctt taagtgttat gttattcttc ttgacggctt gtgggcaaga agatgacaaa
    13441 aaaatcacgc tgaacatggc ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa
    13501 gtaatcgagc tttatgaaga aaaaaatccc catgtgacga tcgattatga attttatgat
    13561 tatgaaggat attacacaaa attaggtaca ctagcagcat cagatgatat ctgggacatt
    13621 ttccaactga acaatcaatt tcctcaatac atcaatcaga ttgaaccatt agatgaatat
    13681 gtggagcaag ggataattga tatttctaat gcagatgaat cctatttagc aacgacgact
    13741 tacgacgggc aattagttgg actgtctaac ggggtgaatt cgtttgcgat tgcttacaat
    13801 aaaaatatat ttgaacaatt aaatatccct gaaccagctt caaattggac atgggatgaa
    13861 tttgaacaaa tcagctatca aatcactgaa gaattaggaa tctatgctat ttcaagattc
    13921 gaagatttta tagctggctg tattattcaa ataccacaag ttgagaaagg attgaatttt
    13981 tataatcggg aagatctatc taacagcttg ggatttgaaa atccagacta tctaacagat
    14041 tttttccagc tgcgtaaaaa tctggtaaac aaaggggccc atccagaacc aggtggtgga
    14101 gctgcaagta cgacggatct gtcttttcag gcggttaggg atagagaagc ggcaatggtg
    14161 tttctatcaa gtaaccagtt gacagaaata ttgtcagggg ctcctgaaga tatggagatt
    14221 cagttgatta atccacctcg cagaaaagca gatggagaat ctggtatccc tttacgttct
    14281 tcgcaaatgc tgagtatggc aaaaagctca gcccataaag aagaagctgc taagtttatt
    14341 gatttctttc aaaacagtga agaagccaat gaaattttaa aaggagaacg tggtgtacca
    14401 atcatgtcga atattcgaga acagctcgct cgtgcagatg atgcaacttt gcaagcgaca
    14461 tttgattatt tagaaatgat ttccgatatg gacaacggcg aagtgaatgt ctttgatagt
    14521 gctttgaatc cggaaattca agaccaatac aatcttttag ttgaaaaagt catttatgaa
    14581 gaagtaacag cagaagaagc agcagagagt ctttatgagt ttgcgtcaaa cattctagga
    14641 tagcagcagg aggaaggcat gaaaaaagaa aaactaaaac ggttcttgga tcaagacaat
    14701 gtggtcggtt acgtatttgc ttctccattc atcctaggat ttctattgat aacagtgata
    14761 ccgatgatct tgtcacttta ttattcgttc accgattatc gtttgggaaa tccagtctca
    14821 tggatcggct tggataatta tataaagatg ctgagtgatg cccgtttttt gaactccatc
    14881 cgagtcactt ttctctatgt atttgcatct gtacctgtga aactgatctt tggtctattg
    14941 attgcttatc ttttaacgag gaatatcaaa ggaacgactt tctttcgttc tttgtattat
    15001 gtgcctagtt tgatcggtgg cagtatttct atcgctctag tttggcggga gttgttttct
    15061 aaaaacgggt tagtgaatat tttcctggaa cgattaggcg cacctgcgct ttcctggttt
    15121 agcgatcaaa aactggtcat gatccctctt gttttgatgt ctgcttggca attcggttcg
    15181 gcaatgatca tctttgctgc aggtatcaaa gagatcccag gctcgtatta tgaagcggca
    15241 gagatcgatg gagcgaacaa atggcagacg ttttacaaga tcacgttacc tcttctttcg
    15301 ccgatcattt tgtataactt ggttatgcag acaatcgcgg cgtttatgac ttttacgcag
    15361 gcatttatca ttacccaagg tggtccgaat gatgccacga acttttatgc tctttatgtg
    15421 tataaccaag ctttttcttt taattctatg gggtatgcta gtgctatttc gtggatcatg
    15481 ctgttgatta tgggagctgt cacctttctg attttcaaat catcaaaaaa atgggtcgtt
    15541 gtgtgaatga gaggaggaag aaatgaataa tacagtaacg aaatggtttc aatacggttt
    15601 tattttgcta ttaggtctag tcatgattta tccggtctta tggatgatcg caggttcttt
    15661 taaaacggat caagagatta tgagtggttc gctgaatttg attccagaga cttttcaatg
    15721 ggaaaattac gcaaaaggat gggcggggtt tgctggaatt tcatttttta ctttctttag
    15781 gaattcgttt gtcatctctg cagtttcgac gatagggaca gttctgagct cgacttgtat
    15841 tgcttacgca ttatcgagaa tcgatttcaa aggaaaaaga ttctggttcg tcgtcatgtt
    15901 agtaacgatg atgattcctg cacaagtgat tttgattccg cagtttatta tttacaatcg
    15961 gctgaatttg gttgggacgt atgtgccgct gattttacca catttttttg gacaagcatt
    16021 tttcatttac cagatcatgc aattcatggt cagcatcccc aaagagttag atgaatcagc
    16081 gattattgac ggatgcagca agtatagtgt ctttaccaaa attatttttc ctttgctgaa
    16141 accttcgatc atcacgacga tcatcatcca gttttattgg aaatgggacg acttcatggg
    16201 accattgatt tatttaaaca agccgagatc ttacacggta tcgattgcca tcaaattatt
    16261 tgcagacgct ggatctagta cgacttacgc gagtatgttt gcgatgtcta cgttgtcgct
    16321 gctaccggtt tttctgattt tcttattctt caataaatat cttgtccagg ggatcagtac
    16381 aagcggtctg aagggctgaa tatgaaaaag ccaatagaag agatgtttct ttacaagctc
    16441 aactactatg tctgtcggat ttttttaacc agtcttatca gtaatgctct tttcgcttta
    16501 gttctaggat gtttgttcac tttaggatta gtgctgtctg ctcgtgttgt ctatctgatt
    16561 tattttttga tgatgtatcc ttcttattat ctatctgtcc attacttggc agaaaaaaag
    16621 gtatgttgga ggaaaggaaa aatcaaacgg atgtgtttca tcattacgtt gtttcagctg
    16681 tttgtcatta tttttgcagt tgattggtat ttattagtgg gaaagattgg agcttggtat
    16741 ctgattcctt ttctattgtg tctgttgctt tttgtatccc atagtttttt tagtacgcta
    16801 attgatatct gcaaagaaga taaagcagat agtcttgttt atttgatcaa aaggaatatc
    16861 tggctgttgt caaaaaacgt caaggggttt ctgtggtatt tatgcctttt gtatggactg
    16921 ttctggtgta tacagcatga ttttttactc actgcattac tgtatccagg gctgccattt
    16981 gtcacagtat tttgcttaaa agaaaaggag aatgacttta aatgaagaaa aaatatgtac
    17041 aagtcggact tggtgggaga gcgcgttttt tttatgaggc aattgcctct acgtatcatc
    17101 aaacttgtga actagtcggt ttttgtgatg tatctcaaac aaggatggat tatgcaaatc
    17161 ggatacttgt gaacaagtat ggacagaaaa aagtaaagac atgtatgcca tcacaatttg
    17221 agcaactact ggatgacgta cggccggctg aagtcattat tacgtcagta gatcgaacgc
    17281 atcatgatta tatcattcgt gcaatgaaaa aaggatatga tgttatttgc gaaaaacctc
    17341 tgactatcga tgaggaaaaa gctcaagaaa taataacggt ccaaaaacag acgggaagaa
    17401 gcctgcgagt cacttttaat tatcgttatg caccgtatca cacgaagata aaggaactga
    17461 tagaaagtga tgtgattggt gatgtgtttt ctatccactt cgaatggctg ttaaatacgc
    17521 aacacggggc agattatttc agaagatggc atcgaaataa gcacaatagc ggcgggcttc
    17581 ttgttcataa atctacccat cattttgatc tagtgaattt ttggttgaac agccgtcccc
    17641 aaaaggtatt tgcattcggt ggattaaact tttatgggat gcataatgcg gagaaacgcg
    17701 gagtagaaat attttacagt cgatgctatg gaagcgaact agctaaagct gatccatttg
    17761 ctatagacct gtcagccaat gaggaattgc gagaaatgta tttagaagca gaaaaagaca
    17821 gtggatatat tcgtgaccgc agtgtatttt ccgatgatat ctctattgaa gatacactag
    17881 gcgtaacggt caaatatgaa actggtgcga ttttgacgta ttcgctaaac gcgtatatgc
    17941 cgtgggaagg attcaatgtt tctttcaatg gcagcaaagg acggatcgaa atgaaagtga
    18001 tcgaaaagtc ttacatcaat gcaggtggag ataaaaagga tgaaggagca gccgttcttc
    18061 agtccatcca tgtctatccg atgttcggtg agccatatga ggtaatcgtc gaacaagaag
    18121 cgggcggaca tggcggagga gatccgcgtt tattagatga tctatttggt gaagaaaaag
    18181 aagaagatcc gtggaatcgg gcagcaactc acgtagatgg tattttgtcg attttgacag
    18241 gaatcgcagc aaatcattct attgcttcag gaaaagcagt agtgattgac gagcttgtca
    18301 gcttaaaaga ataagaatag atttacggaa attacttccc gtagtttata aaatcttgcc
    18361 ttattgccga taggctgtct tttgaacact atttattcgg aaaaaggggc tatgacatgc
    18421 tttttgtcat gccttctttt tatgtgggct gcttttttag ataggagaaa ataaaaataa
    18481 ctatttaaac ccgaacaaaa aggaataatg cattaaaaat cattagattt tacaaaaaaa
    18541 taagtaaacc acgttgtcag tttcagaaag tagtgttaga ctgttttcaa tatttgaaac
    18601 aacatttttt attcaaatat ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg
    18661 aatttacact attattagtt ttacatacct acttatatat cgtcataata tggatgacag
    18721 tttctagcca gtaccgtaaa tgctggacta taagtaaaag attggctatt tcacccttct
    18781 tttcttatga taaagtggag ggtgtttttc tttaacagaa acatagtagg tttttgcaca
    18841 aaagcggtta tttcggtatt aataaatgat aaaggagcaa ataaagtgaa agaattagtt
    18901 gaacgtattc aaaaagacgg acgtatatta ggcgagggcg tattaaaagt cgacagcttc
    18961 gtcactcacc aagtagaccc tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta
    19021 tttaaagaac aaggaatcac gaaagttgtg accattgaag cttctgggat cgcaccagct
    19081 ttatttgctg caagagaatt agatgttcca atgatcttcg ctagaaaagc aaaaagtttg
    19141 acgatggatg aagaattatt gacagcttct gtttattctt ttacgaaaca agtgacaagt
    19201 acgatttcta tttctcgtaa atttttatct tcagaagaca aagtattgat tatcgacgat
    19261 ttcttggcaa acggacaagc ggcaaaagga ttgatcgaat tatgccagca agctggagcg
    19321 caagtagaag gaatcgggat cgtcatcgaa aagtctttcc aagatggacg tcagttactt
    19381 gaagaaatgg gattgagagt cgtttctctt gcgcgtattg cttccttgac gaatggacaa
    19441 gttgagtttc ttgaggagga cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg
    19501 cggttttagg gctgcagcat ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt
    19561 tgatcggtac aggactaggc tttaatcaag aacagatgac ttatttgatt tcaattgata
    19621 tctttatgtg tggattagcg acattattgc aattgacagt taaccgattt ttcggaattg
    19681 gcttgcctgt cgttttagga tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa
    19741 ccaatgaagg agtaggagct atctacggat ctattatcgc ttctgggatc tttgttgtgt
    19801 tagtttctgg tgtcttttct aaaattaaaa aactattccc tcctatagta acaggaacgg
    19861 ttatcaccgt tattggtttg actttgattc ctgtagcaat cgagaaaatg ggtggaggta
    19921 atgcaacagc agctgatttt ggtgacaaaa caaatctgct acttgcattt gtgacgattc
    19981 tcctaattgt aggcgtgcaa atgctggcaa aagggttcat tcgttcaatc gcggtattga
    20041 ttggtttagt cggaggaagc atcttagctg ctttcatggg aatgattgat ctgagcgcga
    20101 ttggagatgc gccgttgttc catgttccac aaccctttta ttttggtaaa ccaactttcg
    20161 acgtatggtc gattttacta atgatcctca tttccatggt cagcatggta gaatcaacag
    20221 gtgtatattt tgcacttggt gacattactg ggaaaaatgt tggagaagaa gaattgaaaa
    20281 agggatatcg tgcagaagga cttgccgtta tcttaggcgg tattttcaat acattccctt
    20341 atactggctt ttctcaaaat gtcggattgg ttcaactttc gggaatcaaa acaagaaaac
    20401 ccatctattt ctctgcattc ttcttgattg ttttaggatt attccctaaa atcggtgcgt
    20461 tagcacaaat catccctgag ccggtacttg gtggaggaat gttagtcatg ttcgggatgg
    20521 tggcagtcca aggaatccgt atgctgttgg aagttgattt cagcaatgat aaaaatctat
    20581 tgattgttgc tgtttctgtt ggatttgggc tgggtttcaa tgttacgccg acattattca
    20641 gtcaaatgcc gcaaacattg caaatgttca ctggaaatgg catcgtaatg agtagcatta
    20701 cggctatcat attgaatttg atctttaatg gattgaaaaa agatgaagaa atattggaag
    20761 aagccttcaa gaagtaatag agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt
    20821 gttagagaaa caaacggaaa aatagttcct cgtattttgc cgtttgtttg agctaatacc
    20881 gagggtcagc tgattgaaca ccgttgaagg aagaggtcgt gagcctgccg ttcatctccg
    20941 agtcacaagg aacaatttca aaaaaaagct tctcatgttt gttgaatatt gttccttgtg
    21001 accgaggagt tggcaggaga acaccgttta tccataaaaa ggaatgtgac ctaacttttg
    21061 tcacattcct tttttttatc tttaataaac acgaacaata aaatgttttt ctttgttaac
    21121 attcgaaatt tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg
    21181 ggaggaaaaa caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa
    21241 acaatgaaag aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc
    21301 gtttctgctc atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa
    21361 ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca
    21421 tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg
    21481 gattcgcttc tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc
    21541 ggaaaagcgg gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat
    21601 ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa
    21661 agtagtgatc aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg
    21721 cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt
    21781 gctggatcct gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt
    21841 ctatgatgac acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga
    21901 attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca
    21961 agggacagac ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac
    22021 gaataacatc gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc
    22081 gattgacggg atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa
    22141 ggggcagtat gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga
    22201 aggaacctgt gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc
    22261 aggaaatggt caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa
    22321 tattcttcat gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga
    22381 gcgtattgcc cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat
    22441 gtttttaaca aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc
    22501 tggacattat tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat
    22561 ttgtggttgg ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt
    22621 tttgggagaa caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt
    22681 ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt
    22741 gacagaagac atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag
    22801 gagaaaaaac atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga
    22861 gaacaaatac cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact
    22921 tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga
    22981 tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt
    23041 ttctgaaaca ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt
    23101 cgtagatact gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt
    23161 gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat
    23221 gggtcggaca cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg
    23281 gtattcagaa atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc
    23341 aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta
    23401 tgtatgtaaa caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga
    23461 tctacatgct gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc
    23521 aacagagata cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa
    23581 aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat
    23641 ggctggtcta gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt
    23701 atggcatgaa agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac
    23761 gattttattg aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt
    23821 tcctgaaaac atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt
    23881 tatgctgaaa ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc
    23941 aaaaactgcg tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc
    24001 aaccatcaca tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca
    24061 tttgaaaaac gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg
    24121 caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg
    24181 ttctgcttct tttagtttat tatattttct tatcaataat aggaacctga ttttaaaagc
    24241 ggctacctct ttaccgtgcc tctttacata caaaaaatag aggaattaag cgtaaatggc
    24301 ttggctatta taactaaaat gctggttatg gagatgttgc agcaagtttt cgagaaaagc
    24361 gtattccatc tcttgtccat tgaataagat ctggatacga taatgatttt tgaaacgctt
    24421 ctgttcatga atctgactga ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt
    24481 ccttttacgg cattcagcac agcgcttggt tgctcggcgg atttttcgga atgtataaaa
    24541 actagtatct ttattgcata aataaacgtc tttgatcgac agcaaatcat tatatagaca
    24601 ggaacgttct tcagctgtta attctgataa aaaaagctga ccttgtttta ttgcatcttc
    24661 catcggcaag tgcctcctcg aacccattat ccaaattttt agttaattct aatataccat
    24721 cattaagtat aaatgtaaac gcttgttgat ggtataagat tttcgtttct gataaaacac
    24781 gaacattaaa agaatctaat gccttaaatg tacatatttt tgttgattta taaaaagatg
    24841 attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa
    24901 taaaaaggag agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa
    24961 atatatctta cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg
    25021 ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca
    25081 agtgcaattt tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg
    25141 tctaaaaccg aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga
    25201 aatattgcag caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc
    25261 aaaccaatca ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa
    25321 gagcatatct atttcttaca acttgcaaag ccggaagaaa tcagtgagct gaaagcccaa
    25381 gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta
    25441 gatttcaagc tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca
    25501 ccggatactt gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac
    25561 cgcagagatc taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa
    25621 ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag
    25681 attcagtgtt agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg
    25741 aaacaatcga agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag
    25801 aaccaatcga tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg
    25861 aaacataccg atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc
    25921 gtttttccag gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc
    25981 gctgatgccg aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta
    26041 ctgccgggag gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct
    26101 acaatcatgg aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc
    26161 aatggattcc agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag
    26221 tctttgcatt ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact
    26281 tcagagtatg aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat
    26341 ttctgtgacg aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat
    26401 aatgacaaca tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat
    26461 gtgttaggaa tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat
    26521 gggaaacggt tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa
    26581 acattgaaag aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga
    26641 ttgactgatg aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat
    26701 acggaaactg gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa
    26761 ccggttctac gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc
    26821 gcaggcatcg tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat
    26881 catccttcag ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga
    26941 gatattttca gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa
    27001 ttagataatc caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac
    27061 ggcaattgta tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa
    27121 ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa
    27181 gggcaagccg ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac
    27241 ggtatccacg gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt
    27301 tctgctgtac aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa
    27361 ttaatcttgc atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt
    27421 gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat
    27481 gaagtcccac aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa
    27541 gaacggatgt tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag
    27601 aagtatgatc tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta
    27661 tatcatcaag gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca
    27721 gtttatcata aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct
    27781 tatcagccag ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg
    27841 attgcttcaa aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt
    27901 gtccgtccag gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct
    27961 atgaccacag actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata
    28021 gcagtggcag aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc
    28081 gactgcctga actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca
    28141 gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc
    28201 tctttatata atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc
    28261 ggattgattg aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta
    28321 atctatatat taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag
    28381 cttggaagaa tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa
    28441 gagttagtat tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa
    28501 ggtggcttgg caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta
    28561 cagtttgaat tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca
    28621 gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca
    28681 ggtaaggtga cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata
    28741 gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga
    28801 gtttaaatga agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg
    28861 catacttcgg gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca
    28921 atgatcgagg aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag
    28981 atcaaagaaa attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa
    29041 cagcaggcaa tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt
    29101 cgatcggctt agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag
    29161 aggaaaatgg ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc
    29221 gacgaagtga agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag
    29281 gtggctttgc ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg
    29341 gttttcgtcc attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa
    29401 cttgtgcatt agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag
    29461 tgatcatcaa tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta
    29521 tttgttcgat ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg
    29581 tgcatactgc acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag
    29641 atatggtcgt tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta
    29701 gtggaatccc ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc
    29761 agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag
    29821 tagtggaagg taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca
    29881 gacggatcgt ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt
    29941 ctcctccttt gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca
    30001 ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt
    30061 ttttaagtga agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag
    30121 gattgtgtat ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa
    30181 aatatcagga atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag
    30241 tatatgaatt caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat
    30301 gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc
    30361 gctataggag gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc
    30421 cttgtctcgg ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa
    30481 cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc
    30541 gcagagccgt tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg
    30601 gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc
    30661 ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt
    30721 gctgtaggga tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat
    30781 gtgttgcttg gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa
    30841 attttctttg agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca
    30901 cttggcgaag aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc
    30961 aaagaaaaga aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc
    31021 ccgcgaatgt taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt
    31081 ccaatattcg gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc
    31141 ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta
    31201 cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa
    31261 aaacatcgtg tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa
    31321 acggaagcaa ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga
    31381 ttgactggtt gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga
    31441 gtgtaccggc agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag
    31501 caattttgca taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc
    31561 gaatcattgg accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt
    31621 ctcttttacc tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa
    31681 aagaaacggg tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc
    31741 ggcaggaaaa agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc
    31801 atagagtaga gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct
    31861 atctgaaatg acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc
    31921 ccaaggtttg attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt
    31981 ggataaagct gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat
    32041 ggatggccgt gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga
    32101 tctccctagc catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg
    32161 tgtcaattta tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc
    32221 tattgaaaac attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga
    32281 atttgtcacc tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca
    32341 gtatgggcat acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac
    32401 tgcggcatat gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc
    32461 ggaaaaattg acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca
    32521 tcaagccgct gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa
    32581 acaactccat gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg
    32641 gatcatgaag gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg
    32701 tattgggaca gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt
    32761 ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca
    32821 gatgcataag ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga
    32881 attactgcaa aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa
    32941 tcaaaatcga gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct
    33001 ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga
    33061 agcaaaagca ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt
    33121 actggcaaac gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc
    33181 agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga
    33241 tgcttatttt ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat
    33301 cattcagcca ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga
    33361 tctagcaatg gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat
    33421 gaacattttg gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc
    33481 cagtccgaag gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg
    33541 tatcaattgt gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa
    33601 agaggctatt gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga
    33661 tgattttatg gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga
    33721 aggttcgaaa agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc
    33781 cagaagtttt gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat
    33841 cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa
    33901 ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa
    33961 aaaaatcgtc gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg
    34021 taataccaag gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga
    34081 taaagggcca aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa
    34141 gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact
    34201 aggacgctct ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa
    34261 agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt
    34321 gaagagtgac tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa
    34381 gtggcagcaa gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga
    34441 atatgagaca ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc
    34501 tggagtttcc tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga
    34561 agcatacggt gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa
    34621 aacgacagat ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact
    34681 gatcgtgaag taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc
    34741 gccgatttta gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat
    34801 aaggaggagt tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag
    34861 tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag
    34921 cttatgaaaa cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg
    34981 aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc
    35041 aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg
    35101 aatctgtaga aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc
    35161 atcgtccaga tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac
    35221 ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc
    35281 tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata
    35341 ctggaatgat cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg
    35401 acggtagtgt acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat
    35461 accagtatgg attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc
    35521 aaacattaga agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt
    35581 ggattttacg ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa
    35641 tcaaggaaat cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt
    35701 accgtgctgc tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata
    35761 aattaaaaag acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt
    35821 tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta
    35881 aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa
    35941 ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct
    36001 gattgctgca ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat
    36061 gtttggttaa acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt
    36121 tgagcgaccc aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat
    36181 gcatggttca aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag
    36241 aaagaaatga acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag
    36301 cgttgactgg tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag
    36361 acatgctgga gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg
    36421 ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac
    36481 aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc
    36541 aaatcctcat agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc
    36601 gtgatctcaa aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca
    36661 gaaaaattat ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa
    36721 ctggtctcga tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag
    36781 gaaacagcag agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg
    36841 agatgagcgt aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt
    36901 tttttccctg ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta
    36961 agtgagcctt tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca
    37021 gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct
    37081 tgtttcaatt cgctactttt aatcggttta agcagataat catctgcctg tacctggatt
    37141 gctcctttca tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga
    37201 agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca
    37261 gtcagaatga tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca
    37321 gcttcgccga cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag
    37381 ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc
    37441 aattgttttg ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct
    37501 gtcaatcgtg ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt
    37561 gtaaagtccg tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat
    37621 ttctgccagt ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat
    37681 ctcatcaatt ttgcatgtgg taatacgaat aaatcctttg tgacggacgt acttcgttcc
    37741 atggtataca gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa
    37801 aagtgtttct ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat
    37861 gactagataa tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt
    37921 gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa
    37981 gcagtcttgt tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt
    38041 aatctgagct tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt
    38101 ctcccgtaaa gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg
    38161 taattccagt gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc
    38221 tgacatctcg atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt
    38281 ctttttcgca tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat
    38341 gagcgagcgg acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa
    38401 atcttgtaag atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc
    38461 attggtatcc ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc
    38521 cagtgtatcg atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt
    38581 attatttttg atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa
    38641 ctcatctacg gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc
    38701 aaccttcaaa gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa
    38761 aagctgttgc gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat
    38821 ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct
    38881 ccagtcaata atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg
    38941 aaataaaaat atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa
    39001 cggtattggc tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc
    39061 ctgagcgtag cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt
    39121 ctccaggttt gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac
    39181 tatggctgta taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct
    39241 ggtcttttcc tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag
    39301 gtttatggtc gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa
    39361 atccttcgga gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc
    39421 tgatttcctg tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc
    39481 cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa
    39541 tatgtctgtc gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt
    39601 cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg
    39661 tccgtatatc ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt
    39721 aatcttcttt ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct
    39781 ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg
    39841 tacaccccat catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca
    39901 ctttaaatca ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa
    39961 agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa
    40021 gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat
    40081 tagctatact cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa
    40141 gattgttggg actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc
    40201 aggatctgca gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga
    40261 aagatggatc gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac
    40321 agatcttcaa tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat
    40381 tacaaaaggg gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt
    40441 gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa
    40501 ttcagaaaat gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc
    40561 agcttatatt gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt
    40621 atttggtggc tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt
    40681 tttacaacca tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag
    40741 tcaaatcgct acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt
    40801 aagcgcaaat tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag
    40861 tttaggaatc atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc
    40921 tgtgattact ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca
    40981 aacacaaacg atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa
    41041 agcaatcagt gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt
    41101 gaaaactgtc cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc
    41161 agagctgatc gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa
    41221 caaggaacta aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta
    41281 aaaatggcag atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg
    41341 atgattgaac gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca
    41401 ttgatgaatg tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat
    41461 gatggaaagc tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc
    41521 attcatcaag aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga
    41581 aatgaacaag cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa
    41641 ctgctgaata aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta
    41701 gggcaacagc aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt
    41761 ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc
    41821 cgagagttga aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc
    41881 gtggatatcg cagatcgaat caccatttta cgggatggac agacgattga gacactagaa
    41941 aatgaaatga tcagcgaaga gcatattatt cgcggaatgg tgggcagaga tttagttgat
    42001 cgttatcctg aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg
    42061 gtctatcacc ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa
    42121 aaaggagaga ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg
    42181 agtctatttg gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca
    42241 gagatatcag ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag
    42301 gaccgaaaat ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc
    42361 agtttggata aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca
    42421 gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt
    42481 cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat
    42541 attctttttt tggatgagcc aacccgtggg atagatgttg gggcaaaata tgagatctac
    42601 acgattatcg aagaaatggc agcaaatggc aaagctgtct gtcttatctc ttctgaactt
    42661 cctgaaatat tagggatgtg tgatcgaatc tatacgatga acgaaggacg tattacagga
    42721 gaagtctcgc gagaagatgc agatcaggaa gtcttgatga aattaatgac gaaagaagag
    42781 gtgggttaag ttggaacagg caaaaaatct tacggataaa aaagagaaaa aactaaattt
    42841 gaaaagtaga gcagctgatc tttttggtcg ctacagcatg gtgatcattt tagcgggact
    42901 acttattgcg tttcaattga tgacggatgg tattttctgg cgtccattga atataactaa
    42961 ccttgttctg caaaatagcc atatattagt actagctgca gggatgttat tagttgtgct
    43021 cttaggtcat gtggatttgt cggtagggtc agtgatggcg tttgttggtg caattgcggg
    43081 catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt
    43141 aggcgggctg atcggcgcat ggcaaggttt ttgggtagct tatgcaggga taccggcgtt
    43201 tatcgtgact ttagcaggat tgctgatgtt tcgtggactg acacaagttg ttctaggagg
    43261 ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt atttaccaga
    43321 cttaagcgaa ggaagcagcc ttcacttctt gacattgatt ttaggaatga tcatcacgat
    43381 cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt
    43441 ggaatctcca acagctttct tggtaaaggc ggtatttaca gcagtcgttg tactaggaat
    43501 aacttatatc tttgcctctt atcaagggta tccgattatt ttgatcattc ttggtttgat
    43561 cgttgcagca tatgggtttt tgacaaataa aacagtagca ggtcgacaga tttatgcgac
    43621 gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa tcacgttctg
    43681 ggtattcgtc aacatgggag tcatggcagc tttagctgga ttagttttag ctgctcgatt
    43741 gaatgcggca acacctcaag caggtacatc gatggaactg gatgcaatgg cggcagtata
    43801 ttttggtggt gcttcgactt ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg
    43861 cttggtcatg ggtgtattga acaatggcat gtcgatttta ggcgtcgggg tagactggca
    43921 gcaggcaatc aaaggattga tcttgctttt agccgttgtg ctagacttgt ataataagaa
    43981 aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa agactgtgac aagacatttg
    44041 tcacagtctt tttttcgcta aaagcgaaca ttcattgttt cgtttgtata gactaaaaat
    44101 aatatatgtt tttttaccat tcgtataaat tctctttttg ctcttgattt ttttgcttaa
    44161 ttgagttaaa ttacataggg cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt
    44221 ttgctggatg tgaacatcag gaggataatg taatgaaagt atttgattac gaagatattc
    44281 aattaattcc taataaatgt atcgtaaata gccgttctga atgtgataca acggtcacat
    44341 taggtaaaca tacattcaaa atgccagtag tgcctgccaa catgcagacg attattgatg
    44401 aaacaatcgc agaattctta gcggaaaatg gttatttcta tatcatgcat cggtttgatg
    44461 aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca
    44521 gtgttggcgt caagaaagag gaatattcct ttatagagaa gttggcagaa gaatcgctaa
    44581 atcctgatta tatcactatc gacatcgcgc atggacatgc taactcagtt attgatatga
    44641 ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat tgccggtaat gttggtacac
    44701 cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc
    44761 caggaaaagt atgtattaca aaaatcaaga caggctttgg gactggaggc tggcaactgg
    44821 cagctcttag atggtgtgcg aaagcagctc gcaagccgat catcgctgac ggtgggatca
    44881 gaacacatgg cgatattgca aaatctgtcc gttttggtgc gacaatggtc atgattggtt
    44941 cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt
    45001 ataaagaata ctttggttct gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag
    45061 gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga tacactagtg gaaatgcaac
    45121 aagaccttca atcatctatt tcttatgctg gaggaagaga tctggaagcc attcgaaaag
    45181 ttgactatgt catcgtgaaa aattcgattt ttaatggaga cacaatctaa aaaataaata
    45241 aaatattagg atgttgtgaa aaagttgtcc tgcattaagt aataagaaca gccaaacaaa
    45301 aatagttctt tatatgtttt gtttggctgg aattattatc gcagatacaa gcttttgaac
    45361 atcattattc agaataaggg actgtgataa gatttttgtc acagtccctt atgttttgca
    45421 aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag
    45481 aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat
    45541 tatggaacag attatgagag aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga
    45601 atcagaacga aaactagggc aactttttgg tgtgaatcgt tccaccgttg tccatgcatt
    45661 ggaagagctt agggagcttg gggtactaac gagcaaacgt ggcagcggat ggtttgttaa
    45721 tcagactgaa tgggggaaat tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc
    45781 tcgttatgag cagacggact ggtatgagca aaaaataaga gaagcaaagc agatccaaaa
    45841 aacatcgttt cttgatttat acgctagcga gatgccagat gagcttttgc cgaattttga
    45901 gtttccttct tattctcttg aagaaatctt agcggaagaa aaagaaatgg atcttttagg
    45961 ctatactccg ttacgtaaca aaatcaaacg gtatttggaa agtaaatttg aattggcatt
    46021 ttcccccaac cagcttcttg tgactggtgg cggtcagcaa gcaatctttt tgattttaca
    46081 aacgattctt tcagctggag aggggatagc tgtagagtca ccttcttttt tttaccgatt
    46141 acctcttttc aaagcttcag gaacgcgttt atttgggata ccgatggatg aagaagggat
    46201 cgacttagaa aaactgaaag aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa
    46261 cccgaatttt caaaacccta caggaaaagt gatgagtcaa aaacgacgag aaaagctagt
    46321 agaattgtgt cgacagtatc ggcttccaat tattgaagac gatgtattta cggatctttc
    46381 atttctaaac caagaagaag tccttcctat acgaacgatt gatccggata atgtgttata
    46441 cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa atcggttggg tgataggacc
    46501 tgagactttt atctcgcaac tagctcaagc gcaagaaatg atggagtttt cgatgaatat
    46561 ccttacacaa atcacagtgg ctaatgtatt taatgaagca attgatttga aaatgcaaga
    46621 tgtaagaaaa aagttgaaag aaaaaagccg actgataagc gcttggggag attcacaatc
    46681 gtttttcaag cttcattcaa ttttaggtgg ttactatgcc tggattactt gggatgggaa
    46741 aaaactagac cccgaactag cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag
    46801 tctacttttt gggcaagata taaatggaat cagaatcaat tacagtaggc tggatgaaaa
    46861 ccaaataccg ttgttttatt caaagatgaa aattctagaa aagtggttaa cagaataatt
    46921 tcacaaagta aatagattaa agaatataac agcttggatt taaatttcaa gctgtttctt
    46981 ttttttcgga aaaagaatac gatataaaga aactaagaaa aaaccattat ttgtacgtcc
    47041 gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac tgtataacac caacgttttt
    47101 aaatagaaac agatggttgt aagtttgatt agaaaataga aaaaatgcag atgaaatata
    47161 aaacgctttc cttattgtga ttttattcac taatgctgtt aatatgcgga ttggcataat
    47221 ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt ctcataaatc catgataatt
    47281 tattaatgaa gacggttaaa tagtgataca gttttattca ctgtttcaat cgcagaatga
    47341 agagattttc ttatagatga gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga
    47401 tcgacttcca agcactgcta gatgaagtca atgcagattt cccagtatat caagcacttg
    47461 atcaagatgg aaacgtagtt aacaaagatt tagtacctga tttatccgat gatgaactcg
    47521 ttgaattaat gactcatatg gtatggtctc gtgtattaga ccaacgttca acagcgctga
    47581 accgccaagg acgcctaggc ttttttgcac caactgctgg acaagaggca agtcagctgg
    47641 ctagtgctta cgcatttgat aaagaagatg ttcttcttcc aggttatcgg gatgtacctc
    47701 aactagtaaa acatggttta cctttatctc aagctttttt atggtctcga ggacatgctg
    47761 caggtaattc ttatccggaa gaattgaaag ctctaccacc acaaattatc atcggtgcac
    47821 aatatgtaca agcagccggt gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg
    47881 tcttcactta tactggtgat ggcggttcat cacaaggtga tttttacgaa gcaatcaatt
    47941 tcgctggcgc ttaccatgca aatgccgtat tttatatcca aaacaacggg tatgcgatct
    48001 ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc acaaaaagct gtcgcagctg
    48061 ggattcctgg tattcaagtc gatggaatgg atccgcttgc agtatatact gtatctaaaa
    48121 tggcacggga atgggcgatc agtggaaatg gaccagtcct aatcgaaaca ctgacttatc
    48181 gttatggacc acatacacta tctggtgacg atccaacccg ctaccgtaca aaagacacag
    48241 atgacgattg gcagaaaaaa gatccattga tccgcttccg taaatatttg acggaaaaag
    48301 gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag
    48361 cagctattgc tgaagccgac aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca
    48421 tgttcgaagt atctccacaa tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt
    48481 cgaaataatc atggcacaaa aaacaatgat ccaagcaatc acagatgcct tagcacttga
    48541 actagaaaat gacgagaatg tcgttgtttt tggtgaagat gttgggaaaa acggaggagt
    48601 tttccgggca actgaaggat tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc
    48661 tttagctgaa tctggaatcg caggattgag tttcggtctt gcgttagaag gatttcgtcc
    48721 agttcctgaa atccaattct ttggttttat tttcgaagca atggacgaag tagtagctca
    48781 aatggctcgt acaagatatc gaatgagcgg cactagaaat ctacccatca caattcgttc
    48841 gccatttggt ggcggtgttc atacaccaga attgcattcg gataatttag aaggtttgat
    48901 cgctcagtct cctggtattc gagtagtgat tccttccaac ccttatgatg caaaaggact
    48961 attgatttct tctattagaa gtaacgatcc ggttgttttc ctagaacaca tgaaactata
    49021 tcgctcattc cgagaagaag tgccggatga agcttacgaa gttcctttgg ataaagcagc
    49081 agttactcgt gagggaacag atgtatctat tatcacatac ggagcaatgg tgcgtgaagc
    49141 aatcaaagca gcagacaatc ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg
    49201 tacagttgcg ccattagatg tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt
    49261 agttgttgtc caagaagcac aacgacaagc gggcgttgcc gcgcaagtag tttctgagat
    49321 ctcagaacgc gctatccttt cattagaagc cccaatcgga cgagtttctg cacctgatac
    49381 agtcttccca tttggacaag cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga
    49441 caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga cgaaacaaag tcatggctta
    49501 tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt
    49561 cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa
    49621 atcagtagaa gaaatcccgt cacctgttac aggaacggtt aaaaatgtga ttgttccaga
    49681 aggtaccgta gcaaatgttg gggatgtatt agtagaaatc gatgcaccag gacacgaaga
    49741 caatgaaggg gacagtggtg tagccgcaga atcccaaact ccagctaaac cggctgctga
    49801 acccactgtt gatacagaat cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt
    49861 accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg
    49921 agatacgatc aatgaagatg atacattact agaagttcaa aatgacaaat cagtagaaga
    49981 aattccgtca cctgtcacag ggacagttaa aaacgtgatt gttccagaag gcactgtagc
    50041 aaatgttggg gatgtattag tagaaatcga tgctcctgga cacaatagtg caccgtcaac
    50101 tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt
    50161 cgttgaagct gccgatccaa ataaacgtgt attggctatg ccatctgttc gtcaatttgc
    50221 ccgtgaaaaa gatgtagata tctcacaagt aactgcaact ggaaaaggtg gcagagtaac
    50281 aaaagaggac atcgaaaact tcttggcagg tggtccaagc agtgcaccag cgaaatcaga
    50341 agcgccagaa gcggcagcac cgaaagaagc agctcctgca gcagaaagca aaccagctgc
    50401 accagcaaaa ccattcaagt ccaacttagg agacttggaa gaacgtgtag caatgacgcc
    50461 aacacgtaaa gctatcgcta aagcaatggt caacagtaag catacagctc cacacgtgac
    50521 attgcatgat gaagtagaag tgtctaaact atgggataat cggaaacgat ttaaagaagt
    50581 tgctgctgca aatggtacga aacttacttt cttgccatat gtcgtgaaag ctttaacagc
    50641 gactgttaag aaatacccag tattgaatgc atctatcgac gatgcaaacc aagaaatcgt
    50701 ttataaacat tactacaata tcggtattgc aactgatacg gatcacggac tatatgtacc
    50761 aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa
    50821 agcaaaatta gctcatgacg gcaaactatc tgcagaagat atgcgcaatg gaacaattac
    50881 gatcagcaat atcggatctg taggtggcgg ttggtttaca ccagtcatca actatccaga
    50941 agtagctatt ttaggtgtag gaacgattgc acaacaacca atcgtaaatg ctgaaggcga
    51001 aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc gatcaccgta tcgtagatgg
    51061 agcaactgcg caacaagcaa tgaataatat caaacgtctt ttagctgatc cagagctatt
    51121 aatgatggaa ggatgataaa caaatggtag ttggagattt tgctgtagaa ttagatacag
    51181 ttgtaattgg tgcgggacct ggtggctatg tagctgccat ccgcgctgct gaaatgggac
    51241 aaaaagtagc aatcattgaa agagagtata ttggcggtgt ttgtttgaat gtcggctgta
    51301 ttccttcaaa agcactgata gctgcaggac accattatca agagtcacta gattctgaaa
    51361 tgtttggagt aactagtgaa aacgtcaaac tagattttgc aaaaacacaa gaatggaaag
    51421 aaaacaaagt tgtccatacg ttgacttctg gtgttggttt cctattaaaa aaacataagg
    51481 ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca cacactacgc gtcattcatc
    51541 cagattcagc tcaaacttat tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa
    51601 tcgagattcc aggatttaaa ttcggaggac gtgtgttaga ttctactggc ggattagcat
    51661 tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg tgtgattggt gccgaattgg
    51721 gcggagcgta cgcaaactta ggagcagaag taacgattct tgaaggctca ccacaaattt
    51781 taccaactta cgaaaaagat ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg
    51841 taacagtcgt aacgaatgca atggcgaaag aagcagtcga taacggtgac agcgtgacag
    51901 tgaaatatgc agtggacggg aaagaagaat ccgttactgc tgattatgtg atggtcactg
    51961 taggtcgtcg tccgaataca gatgatatgg gcttagaaca agcgggtgtc gaagtaggag
    52021 aacgaggatt gatcacggtg gataaacaag gacgtacgaa cgtacctaac atctatgcca
    52081 tcggtgatat cgttccaggg gcggcattag cacacaaagc cagttatgaa gcaaaaatcg
    52141 ctgctgaagc aatctctggc aaaaaagtag ctgtagatta taaagcaatg ccggcagtag
    52201 ccttcactga tcctgaattg gcttctgtag gtatgacgat caaagaagca aaagatgctg
    52261 gtatcgaagc aaaagcatat aaattcccat tttcagggaa cggtcgggca ttatcgcttg
    52321 gtaaaacaga aggattcatc cgtctagtta cgacgatcga agataacgtg cttattggcg
    52381 cgcaaatcgg tggagtcggc gcaagtgata tggtctctga attagcttta gcaatcgaat
    52441 caggaatgaa tgctgaagat atcgccttga cgattcatcc tcatccatct cttggcgaaa
    52501 tcgtcatgga tgcttcggaa ttagcattag gtttaccaat ccatatttga tttgaataat
    52561 acaggcgaaa cgcaactgaa aaatcagttg cgttttgttt tttagaatta tcaataatat
    52621 tattatgtaa acttaaaaat atgacaactt cttggtttat taccacttcc tatgctacac
    52681 tgaagaggaa tatgaaatag gagagttgaa gatgcaagaa tatcagtatc cacttgattt
    52741 agattggaca acagacgaaa tggtcattgt aatgaagatg tgggaagatc tggaacgtgc
    52801 gaatgaaaaa gggataaaca atgaagcttt tttgaagact tatcagcagt ttaaaactgt
    52861 tgtcaaatcc attggtgagg aaaaacgatt gggacgggaa tttgaaaaag cttccggtta
    52921 ttctttatat cgtacagtgc agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa
    52981 cgggtgagat aaatggaaca gatgattgat catatcaagt cctggcttga agaagctggt
    53041 gagaaaatca aatcttctca acaaacatta acaatctcgc aaaaatcaaa tcgaacggat
    53101 ctagtaacaa atgtcgatcg aaacatccag aattttctaa tcgaaaaaat ccattccttt
    53161 gatccagagg cgaaaatatt ggcagaagaa gaagggtgta atcaattatc ggatctcgat
    53221 ggaagagtct ttatcatcga tcctattgat ggcaccttga attttgtgat tgaaggggaa
    53281 aatttttgta ttatgctcgc tgtttatgaa tccggcgttg gaaagcttgg tttcatttat
    53341 gacgtgatga gaaatgaact tttttggggt ggaaaaggaa taggcgtgta taaaaatgat
    53401 gagccattgc ctagaccagc aaataaatcc ttatcggaag gcttacttgg tgtgaatagt
    53461 tatcttttcg gtcataataa atatggaatc cgagaaattg cggaaagcag catggggaca
    53521 cgtatgtacg gatgcgctgg tcttgaacta gtggcaatct tgaaaggaaa ccacattgct
    53581 tatatttcaa accttagccc ttgggattat gcagcgggaa atgtcttatt ggaagagttt
    53641 ggtatggtaa caagcggtct ttttggtact cctttgacat ttgaaaaaag ggaatactat
    53701 ttagcaggga cttcttgtgc atatgcagaa atcgcagacc ttttgaaaaa agaaggctga
    53761 aaaaaaggta gaaatcattc acaaaaatga taaaaacgag caaaagatta attttctcga
    53821 aaaaatgaaa acattttact atatttcatg aaaaaagtct gctataatta gcagtcgagt
    53881 aaaatttgcc ctgtcataaa gaaccaataa aaaaggagtt agttacattg aattatagaa
    53941 acgatatccg taacgtcgca attattgccc acgttgacca cggaaaaaca acattagttg
    54001 atgaactatt aaaacaatca gatacattag acgcacatac gcaattgcaa gaacgtgcga
    54061 tggactcaaa tgcgctagaa aaagaacgtg gtatcacgat tttagccaaa aacacagctg
    54121 tagactataa aggtattcgc gtaaacatca tggatactcc aggacacgcg gacttcggtg
    54181 gcgaagtaga acggatcatg aaaatggttg acggcgttgt gttggtcgta gatgcgtatg
    54241 aaggaacaat gcctcaaaca cgttttgtat tgaaaaaagc actggaacaa catatcacac
    54301 caatcgttgt cgtaaacaag attgacaaac cttctgctcg tcctgagcat gtcgtagatg
    54361 aagttcttga actattcatc gaattaggag cagatgatga tcagttagac tttcctgtga
    54421 tttatgcatc tgcactaaat ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac
    54481 caaccatggc acctattttt gatacaatta ttgaaaaaat ccctgctcct gtcgataaca
    54541 gcgacgaacc attacaattc caagtttcat tacttgacta caacgattat gtaggtcgta
    54601 tcggaatcgg acgtgtattt agaggaacaa tcaaagtagg agaccaagta gcattaatta
    54661 aattagacgg aacagttaag aaattccgtg taacgaaatt atttggtttc tttggactaa
    54721 aacgtctaga gattcaagaa gcaaaagctg gagacttgat tgctgtctca ggtatggaag
    54781 atatctttgt cggtgaaaca gttactcctg tggatcatca agatgcgtta cctatccttc
    54841 acattgacga accaacattg caaatgactt tcttagttaa taattcacca tttgccggtc
    54901 gagaaggtaa attcgtaacc gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa
    54961 cagatgtatc cttacgagtt gaaccaacaa actctccaga tgcatggaca gtttctggtc
    55021 gtggagaatt gcatttgtct atcttgattg aaaacatgcg ccgtgaggga tacgaattgc
    55081 aagtatctcg tccagaagtt attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg
    55141 aacgcgtgca aatcgataca ccagaagaat atatgggtag tgttatcgag tccttgagct
    55201 tacgtaaagg tgaaatgcaa gatatggtcc atacaggaaa cggacaaatc cgtttgacat
    55261 tcttaacacc tgcccgcggt ttgattggtt attcaactga attcctttca atgacacgtg
    55321 gttacggaat catgaaccat acttttgacc aatatctgcc aatgcttcct ggtcaaattg
    55381 gcggacgtca ccaaggggcg cttgtttcga tcgatacagg aaaagcaacg acttattcta
    55441 tcatgagtat cgaagaacgc ggtacagtct tcgtagaacc aggtacagaa gtatatgaag
    55501 gaatgattat cggggaaaac agccgtgata atgatttgac tgtcaacatc acgaaagcaa
    55561 aacaaatgac aaacgtacgg tctgctacta aagaccaaac atctgtcatc aaaaaaccaa
    55621 aacaattgac gttggaagaa tctttggaat tcttgaatga tgatgaatat tgcgaagtaa
    55681 caccagaaag cattcgcttg agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat
    55741 ctaaaaagaa aaaataatct gtaaaaggat gaagagagaa accagcttgt tataaaaaat
    55801 agctggtttc tttttttgtc caactctttt ctgtggagaa gtcatctttg tcaatggaca
    55861 taaattcaga aaatacgccg aaagacgttt aaaagacttt taaaaaactt atttctagag
    55921 agtttttgtt tcataataag ataatgtttc acgaaaggag atcaacttgt ccttcgaaga
    55981 aaatcgaagt aaaatggcaa gagaaaggag cgggaagatg aacgacttat taaaacaata
    56041 ctttggctat gatgagttta gacctggtca aaaagaaatt atccagaaag tcattgatca
    56101 ggaaaatgta ttaggaatca tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc
    56161 agctttacta ttagatgggc ttactgtggt tgtgtcccct ttaatctctt tgatgaagga
    56221 tcaggtggac gcggcgaatc aattaggtat acccgccact tttatcaata gctcgttaga
    56281 tggttacgaa acagctagac gcttccaaga aatcgataga cagcagtacc gtttacttta
    56341 tatcgctcct gaacgtttta tcatgccgga ttttatccaa gcaatgaagc gatggaacgt
    56401 ccgtatgatc gctattgacg aggcacattg tatctctcag tgggggcatg atttccgccc
    56461 aagttattta cagatggcga accagctaga tcaactgcca aatcgtcctg taattgttgc
    56521 tttgacagct acagcgactg tgcaagtagc tgctgacata aaaagacttt taaaaattcc
    56581 agaaaacaac catattcaga ctggttttga acgagaaaat ctacgatttc aagtaattaa
    56641 agatcaaaaa aaagaacaat atctaatcga atatttaaag atcaacaaaa atcaatctgg
    56701 tatcatttat gcagcaacga gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt
    56761 cgatttttcc gtgggtagat atcatggtgg tctaaatgag aatgagcgaa cagaaatgca
    56821 agaagccttt ttatacgatc gcttgcagct gattgtagcg acaaatgcat ttggtatggg
    56881 tataaataaa agcaatgtcc gttttgttat ccattatcaa attcctggct ctctagaagc
    56941 atactatcag gaagctggtc gggcgggaag agatggtctt tccagtgaag ccatcctgct
    57001 tttctctcct caagatatcc aagtccaaaa gttttttatc cagcagtccc aaagagaaga
    57061 agggcaaaag caaaaagaat atgaaaaatt aaaagcgatg acagaatacg tgtatattga
    57121 gtcctgtctt cagcaataca tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg
    57181 ttgtggtaac tgtttagatg acagagaaat agtagaagta acgacagaag cacagatggt
    57241 tctttcttgc ttaaaacgta tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt
    57301 agcaggatcg aaagaacaaa agctgcaagc tttaggtttt ggacatttat ctacctatgg
    57361 actgatgaaa aaccaatcgc aaaaagaaac gatgcagtta attgaatatt tgatatcgaa
    57421 tggctatctt ctgaccatca atggtgagta tcctgttttg aaagtaacag aaagagggat
    57481 acaagtattg aaaggacaag aatctgttta tcgaaaggaa cccaagaaag tccagcagct
    57541 atcggatgaa gaaaccgata cactatttga agtgctgcgg gaactgcgaa cggatctagc
    57601 atcagaagca ggcgttcccc cgtatgtcgt attttcagat tccacattga aagaaatgag
    57661 ccgaatacgt ccatcaagca gattggagat gcttcagata aaaggagttg gccaaagcaa
    57721 gcttgataaa tatggcgaag cctttttatc tcggataaaa aatgcggact ctaacgttct
    57781 ggataagtag aaagaacgaa tacgtaaaaa tgaataaaaa caactggtta aatctgtttt
    57841 ttttgctcct tatgaaaatc aaatatgtta taattaagga attagaatgg gaggtaagca
    57901 cagatggatt ctttgtcgaa aaaacttgcg atccaagtat taaatgatga agcagaacgg
    57961 atcaaacgat taatccgaaa tcaaaacaac agtctctgta tctcacaatg caaagccttc
    58021 gaagaagtgg tagatacaca gatgtacggc ttttcccggc aagtcggttt tgctgtccgt
    58081 ataggagtaa tcggaaagaa cgagggccag ctgatgttaa gtgagctaga acgagaattg
    58141 aatcagttat atagtgaggt ttaccaagaa atttatgata aaaaagaagt tggcaaggag
    58201 gaatagactt gcctaagaaa gtgaaaaaaa ggcatctatt agactatagc atcttgattc
    58261 cctatttaat tttatgtgta ttgggattga tcatggtata tagttctacg tcgtatctat
    58321 tattagaaaa cggacaaaat ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc
    58381 ttagcttgat tgctatcgca ctactttata aaatgaaaac agatgttttg aaaaaccagc
    58441 gtttgatcat ggcagccatc gcagttttga cgattttgct gctgattgtt gtgttttttg
    58501 gtaaggagat caatggggcg aaaggttggc tgcaaattgc aggattctcg atccaacctg
    58561 ccgaatacct aaaaatcatt tctatctggt acctgtctct gactttatct aaaagacaaa
    58621 acagtgtaca aaaaaatttt cttgggactg tgaaacgtcc acttgcaatg gttatcggac
    58681 ttactgcatt agtcgctatt cttcccgatt ttggtaatgc ggcagttatc tttttgatta
    58741 ttttggttct gcttttagcc agcggagtca attatgtgta tactttgatt gtaggagtag
    58801 gaggcttttg tctaagtact tttacgatct ggctgatcaa tatcacaaac ggaaagatct
    58861 tacccggacg tttacagtat atctataatc gatttgctat ttaccaaaat cctttctcag
    58921 acgaattaaa taaagggcat caactagtca acggctacta tgccatgttc aacggaggac
    58981 tatttggcag gggattaggt aacagtatcc agaaaaaagg atttttacaa gaagcacaaa
    59041 ccgattttat ttatgcaatc gtagtggaag aactgggagt gattatgggg attttgatat
    59101 tagcgttgct attctttatg attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc
    59161 cgttcaattc gcttttgtgc attgggatcg gcgcaatgtt tttgatccaa gtatttgtca
    59221 atcttggagg aattactggc gttattcctt tgacagggat cacgttccca tttttaagtc
    59281 aaggaggctc cagtttgctg atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg
    59341 cggatgaaaa acgcaagagc ttaggtttat aataaaaaaa gatgtaggag tggttcgatg
    59401 aaaaaagttt tagttgctaa caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca
    59461 gaattaggaa ttaaaactgt tggaatctat gcagaagaag atgaatattc cgtacaccgt
    59521 ttcaaagcgg atgaagcata tttagtagga caaggaaaaa aaccgattga tgcgtatcta
    59581 gatattgaag ggatcatttc tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt
    59641 tacggtcttt tgtcggaaaa cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt
    59701 tttgttggac ccaaactgca tcatttagat atttttggag ataaaatcaa agcaaaagct
    59761 gcagctatcg aagcggggat tgcttcgatt ccaggcacag atggaccaat cgcttctata
    59821 gacgatgcct tggagtttgc gaaacaatac ggttatccga ttatgatcaa ggcagctcta
    59881 ggcggcggcg gacgcggcat gcgtgtagct cacgatgaaa agagtgcaag agagggctac
    59941 gaacgtgcga aaagcgaagc aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa
    60001 tacatcgcta atcctaagca tatcgaagta cagattctcg gggatacgca tggaaacgtc
    60061 atccatcttt ttgaaagaga ttgttctgtt cagcgccgtc accaaaaagt agtagaagta
    60121 gcgccttgtg tctctatgaa tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa
    60181 ttgatgaaat atgtcgggta tgtcaacgca ggtacagtag aatttcttgt tgaaggagat
    60241 gatttttact ttatcgaagt caaccctcgc gtccaagtgg aacatacgat cacagaaatg
    60301 attacagata ttgatatcgt aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat
    60361 aaagaaatcg gattgcctca gcaagaagga attaaattaa atggttcagc cattcaatgc
    60421 cgaatcacta cagaagaccc gttgaataac tttttaccag acacagggaa aatcgatacg
    60481 tatcgttcac ctggtggttt tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat
    60541 gtagtgacac cgtattttga ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt
    60601 gaaacagcca ttcagaaaat ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa
    60661 acaaacattc catttatgct caatgtcatc acacatcctg aattccaatc aggaaatgca
    60721 aaaacaacct tcattgatag tacagcaaca ttatttgaat ttcctcgact acgagaccgc
    60781 ggaaacaaaa cgatgaaata tatcggtgaa attactgtga atggttttcc aggtatcgaa
    60841 agtggcgaaa aaccatttta tgaagaacca cgtatgccta aagatctaat cacaagaact
    60901 gattacgtaa cggctaagaa tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg
    60961 atcaaaggac aggaaaattt gttgttgacg gatacgacat ttagagacgc ccatcaaagt
    61021 ctgctagcaa ctcgtgttcg gacaaaggat ttcaagcaga ttgcacgttt gactggagaa
    61081 ggtcttcctg aactcttttc aagtgagatg tgggggggag caacatttga tgtggcttac
    61141 cgctttttga atgaagatcc ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat
    61201 acgttgctgc aaatgctttt cagaggttca aatgccgttg ggtattctaa ttatcctgac
    61261 aatgtattgg tggaatttgt caaagaagca gcagcgcaag gaatcgatgt atttcgaatc
    61321 tttgatagtt tgaactggac tccacaaatg gaaaaaagta tccaagctgt acgggatact
    61381 ggtaaaatcg cagaggcagc tatttgctat actggcgata tcaacgatcc aagtcgggca
    61441 aaatacaatg ttcagtatta taaagacatg gcaaaggaac tagaacaact gggtgcgcat
    61501 atcattgcaa tcaaagatat ggctgggctg cttaaaccgc aggcagctta tcgtttgatt
    61561 agtgaattga aagaaacgac agatctgcca atccatctgc atactcatga taccagcggg
    61621 aatggaatca tcacttattc tgctgcttca aaagcaggag tagacatcgt tgacgtggca
    61681 atgagtgcaa tgagtgggaa tactagtcag cctagcatga gcagcttgta ttatgcactg
    61741 gtcaatggtc cacggctgcc agaaatcacg attgaaaatg cacagaaatt aaaccattat
    61801 tgggaagatg tgcgcatgta ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca
    61861 gaagtatata tgcatgaaat gccaggagga cagtattcga atctacagca acaggcaaaa
    61921 gctgtcggat taggtcatcg ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg
    61981 atgtttggcg atatcgttaa agtaactcca tcatctaaag tagttggaga tatggcacta
    62041 tttatggtcc aaaatgatct tacagaagaa gatatttatg aaaaaggcga aacactaagt
    62101 ttccctgaat ctgttgtgac tttcttccaa ggagaactag ggcaacccgt tggcggattt
    62161 cctaaaaaat tgcaaaagat tattcttaaa ggcagacctg cattgaatga acgtccaggt
    62221 cttttggctg aatcagtcga cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc
    62281 tatgaaccaa aacaagacga agtattaagc tatttgatgt atccgcaagt ctttttggac
    62341 tatcaaaaag catataatca atttggcgat gttacattac tggacacacc aacatttttc
    62401 caaggtatac gtttgggtga aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc
    62461 attcgtttag atgaaatcgg agaacccgat atcgaaggga atcgcgtact gttcttcaat
    62521 ttgaatggac aaagacgtga aatcactatc aaagataatt cgatcatcag tacggtccaa
    62581 acccgattga aagctgaacc gacaaaccga gaacaaattg gagcaacgat gtctggttct
    62641 gttttggatg tattagtgaa aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt
    62701 actgaagcga tgaaaatgga aacggccatc gaagcacgtt ttgacggaga agtcgcacat
    62761 gtatatgtgt cttcaggaga cactatttcg tctggtgacc ttttgattga agtgactgag
    62821 aaataataag cgaaaggtgg ggaaacatga aaagactggg tttgttttgg ctgattctgc
    62881 tattagttat tgtggcattc tacatggaac cagtcttgtt tccgccaaaa cttgaagtga
    62941 aggaaaactc tgatcaacaa atatcaaacc aaatgacaac gtggaaatac caggagctta
    63001 aagcaactgg tttttctact tatatcggaa aaccgatcga tcagctggaa aaggattttg
    63061 gccaagccta tgatcgatta actagcggat ttggttttga aacaagatat tatgaaaata
    63121 aagctagtca tctcacattt gaagcgaaca tccaagacag cagagtcaca actgttaaag
    63181 tactgcaaac agggcctgct gatatcgcgc cgttttattt agggatgacg atgcaagatc
    63241 ttacgaaaat cactactatc tatactaatt tcacttttga gtaccatgat acagaagttg
    63301 gaatcgaact aatggaagag gatatgaatt atcgtccgct aattgctttt gataacgaca
    63361 cttttgctat tttatttttt gatcaaacaa caaacggcct attttcagtt gtgtatttag
    63421 ataaagatag tcttttgaaa ctattgccat atcaagtgtt tggtgaaggc cttccacatt
    63481 atcaagcaga taaatcggct gattgggaat caatcaatcg cgttaaagaa cgaaaaagta
    63541 cgactttatt aaatatgctt agaagagaag acgaacttcc catatacaat caaacacttg
    63601 cctttcaaaa ccacagtcag ctgctactac atgatttttt gacaaggcct gaggaaatat
    63661 tgtcagagga acgtatggct gattggcaaa aatcgctagg cagtgctcaa acgacagtga
    63721 aattctcact gacgaatgat gaattggaga agttaatcag taaacaaaaa ttagaaaata
    63781 ctagtgggct atttattcat ccggcgattg acgctacttt ttcgttttta tattggtaca
    63841 gcgatcctta caaccatgac cgtttcatgg acccgggaac ggattctgta ggcattgctt
    63901 tttctaaaga aaatatgtta gtcttattgc aagaagaaaa cggagaaagt agtgatagca
    63961 gtgatcgtta acgaagagct gtttcaactg gaggatcaat gcctccaagt cgctgaaatg
    64021 ataaaaaaga gtaaaacgat ggcttcttat cttgaacata aacagtcaat ggaagaatct
    64081 gcaaaagttt gccagttgaa agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta
    64141 gcggcatatg gtgagtatgc acctgattat cgtgaaaaaa aacgagttgt tcgaaaagca
    64201 aaaagacagc ttgatatgga tgagcatgtt gcagctttcc gatatagtga aacacagctg
    64261 caaagtctgt tggatcaaat tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa
    64321 gtagatgcag gtagcccgtt ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt
    64381 catgcaatta gacaatagtg aatttgaaat aacaaaacga cgcgggctga tcgtatgggt
    64441 ctacagctta aaacaattaa agaatctgaa aaagttcggc ttgttgcatt atgtctcaag
    64501 aagaatgaaa tatgtcgtgc tttacttaaa tgaagagaca ttggaagaaa cagaagaacg
    64561 aatccgtaaa ttacattttg ttcgaaacgt agaacgttct taccgaccgg atatcgaaat
    64621 gaattttgcc gaaaaaatag gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga
    64681 agagctaaat acagaaatcc gattagctga acatctttaa aaatctctta tgagcagttt
    64741 ttaaataaaa aataacttgg ataacgatcg atttttgatc attgtccaag tatttttttg
    64801 gtatactagt accagtacca tttctaagga gtgagtagat gcggataata tcaggaaatt
    64861 atggcgggcg ccgtttaaaa agtttagcag gggcgaacac cagaccaact acagataaag
    64921 taaaagaatc catattcaat atgattggac catattttga tggagagaca gtattggatc
    64981 tgtttgcagg aagcggtggt ttagctatcg aagcagtatc tagaggctgc agtcatgcgg
    65041 tatgcgtaga caaaaattac caagcgctaa aaatcatcaa agaaaacatc gaaattacga
    65101 aagaaccaga gaaatttacc acaataaaac ttgatgcgga caaagcaatc gaacggctag
    65161 cagcggagaa acaacaattc gactacttgt ttcttgatcc gccatatgca aaacaaaaaa
    65221 taatcgatca aatccataaa atggaagaac tgggactttt ttctaaaacc gcagttatcg
    65281 tatgcgaaac ggataaaaca gtcgaattgc cagaaactgt ggctggattc catcaaataa
    65341 gaaaacagat ttatggcata acggccgtaa ctatctatag aaaagaggga aatcaatgaa
    65401 acgtgcatta ttcccaggaa gttttgatcc ttttacaaaa ggtcacttag atacggtgga
    65461 acgtgcggct aaattattcg atgaggtagt aattggtgtt tttatcaata cttcaaagaa
    65521 aagtttgttt cccccagaag aaagaatgac attgatcaca aaagctgttt ctcatcttcc
    65581 taatgtaaaa gtcatgcatc aagaaaatca attgacggta gaaacagcaa aagagattgg
    65641 tgcagatgcg ttgatcagag gcatacgaag tatcaaagac tttgaatatg aacgagagat
    65701 tgctcagatg aatcatcatc tatatgatga attggaaact gtcttcttat tagcaaaacc
    65761 agaatatagt cacgtgagtt ccagtatttt aaaagaagta ctgcactttg gcggagatgt
    65821 gagcagctac ttaccgccag tgatcaacga agctttagct ggaaagcgtg aatcaaatga
    65881 aagctaaaca atggttgaaa acagtactga ttatcatatt tggaatgttg atcattggat
    65941 ttgtagtagt tcccttgccg tattatgtag aatcaccggg agccacgatc gatctgaaag
    66001 aactaattac ggtcaatgat aaggaagata aaggttctgg gtctttctct cttacctctg
    66061 ttggtatcag gcaagctaca ggtttcttag ctttaaaagc taaaatgacc ccttttgaag
    66121 aattgatcag tgaagaagaa ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc
    66181 aacagtatta tatggattct tcccaaaatg cagcaatcga acaagcgtta aaacttgcag
    66241 acaagcctta taaaatggaa ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga
    66301 atttctttgg caagctttca gttggagata cagtaacaaa agtagacggg aaaagctttc
    66361 aaagtacaga agcatttatg gattatgtaa aaagccaaaa agttggacaa acaatcgaaa
    66421 tcagttattt gcataatggt gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa
    66481 cagataaaaa ggcaggaata ggtatcacgc ttactgacca taccgagatc gaatcagata
    66541 cagatgttcg tattaattct ggctctatcg gtgggccatc agcgggattg atgttcactt
    66601 tagaaatcta tgaacaagtc actggtaaaa acttgcgaca tggtaagcaa attgcaggaa
    66661 ctggaacgat caacagtgaa ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg
    66721 caagtgcgga taaagctggc gtagagattt tctttgcacc agatgatacg atttcagacg
    66781 atgttaagaa agaatatcct gaaataaaaa ccaactacga agaagcaaaa gctgccgcta
    66841 aaaaattgaa ttcctcaatg aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact
    66901 atctagagaa aatgaaatga aactaaataa ttaaagaggg gttgtgacac gagttctgtc
    66961 acaacccctc tttttcgtca aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg
    67021 acgtaattag atgttgaagg gacgattttg ccagagctca ctgtttcttc aattttaaat
    67081 gaaaggagta atgaatgatt tttactggtc attctttgaa aaaagaacaa tgatagaaaa
    67141 aatcagcaag aaaatactag cttcacccaa attgatcatt tggatgtcta tgatagttct
    67201 tatcttttgt atcgcaagtg gttttttatt atcaaatttg ctattttata gagaaacaga
    67261 agagactgct gcaaaagaaa tactttcttc gtttagttcc agtggagaag atccatcagc
    67321 tgaaggaaac gatgtagaag aggatgctcg tccaaaggtc atgtacaccg atatcaaagg
    67381 aagtgtgaag gagcctggta tctattcctt ttcttcagaa gaacgggtct atgatgtact
    67441 aaagcgtgca ggtggattat tagaagaagc ggatagcgat cgaatcaact tttctgcgaa
    67501 gatagaagat cagcaagtcc tatatatccc tgctgtaggt gaagagctgc cagaacatct
    67561 tgatcagttg gcatcctcgg aaggaaaaca gcctacagca gattccgagc ctcccaaaat
    67621 aaatatcaat acagccactc catcagaact tcagcagatt cctgggatcg gatcagtcaa
    67681 agcgcaagaa atcattcgat ttagagaaga aaacggatcc ttccagaaag tggaagacct
    67741 gcaggagatt tctggtatcg gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat
    67801 taaatagaat gattcaaaat cgactggcac gagcaataaa tggacgctaa tggatactaa
    67861 gaataatcca attctaagag cttggcagga cgaggaggta gtatttttgg ataggaaata
    67921 gcttgattgt tcgcaaagaa acattaaaaa aaccaatgct gaatgtctat ctccaaaata
    67981 aaatatctgg gattcatatt atgaatacgg ctgtcagcgg caataacagt caagctctac
    68041 gagaacgctt tgccaaagat gtacttagct atacagcaga taaagtattt attctcattg
    68101 ggacgaatga tctagcagag cataaacaac tttctaaaga aacgtaccaa aaaatctgca
    68161 gtggatgata gttcagctga aaaaagacta tagttctgat catgctttct ttatcacgcc
    68221 accagcagtc gatgagacaa aacaaaaata tcggaataac aagttgatca aagaatatat
    68281 ctctgttctc acagaagtag caaaacgcca tgattgtgaa gtatcgattt ttatacggaa
    68341 tttcttgaac aaatggattc gaagaatttt tataatgggc taatggatga cggacttcat
    68401 ttcggcagtg aagggtagga tcttttgtca gacaggatga ttcgttatat ttaaagaata
    68461 ttttgtttaa gcaatgagta ctttttaatc aacttgaaaa aacggaggaa tataaaacat
    68521 gttagaaaaa tcttcaaaag catgcgatac gaataaagaa aaagttactg ataagtcaca
    68581 taatgaacgc attccttggg accagtactt tatggctcag gcagtactgt tatctttacg
    68641 tagtacgtgt acacgtttag aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc
    68701 tggcggctat aatggagcag tcagtggaga tgtccactgc atagatgaag ggtgttacgt
    68761 cgtggatgga cactgcttgc gaacgatcca tgcagagatg aatgcactcc tacagtgtgc
    68821 aaaacttggt atcccgacgg aaggttcaga aatctatgtc acacattttc catgtttggc
    68881 ctgtacaaag gctttgttgc aagctggtgt gaaagggatc aattatctcc atgactatcg
    68941 aaatgatcct tatgcccaag cgttgatcga acaattaggt gtatctgtac accaagttgc
    69001 tttagactca cagcattttt caaaacttca aaatgaatta acaggaaaac atgtctagcg
    69061 ttttagaaag gcatgttcga aatcggttga tttttccggc aattacgtcg atgattatct
    69121 gtttgttgta ttacactgac aattggggtt atcgtttgct tcttgccgga atttttttat
    69181 ggttttcgct aaaagaagga cgttttatcg cgtacttgtg tattttgtgt agcataacgg
    69241 tatccatttc ttataggcta caagagtatt catttttaga ggaaaaaaca acatctgcgc
    69301 aaattactgt atttacagat acgattcgtg caaatggtaa ttttttgaca atggaaggaa
    69361 ggttatcaga gaataaacaa aagctccagc tttcttatca agtaaacaca gaagcagaat
    69421 tgaaaacatg gcttaacttc cgaggaaagg gaatagaatt gactgctgaa ggcgagttca
    69481 tctctccagc aaaaaagcgg aatctttata attttgacca agaagcttac ttcaggaacc
    69541 atagaatttc tggacggttc caaattcaaa agattgaaca gatcaccatg caaaatcact
    69601 ggcgaaactg gcttcagaga aaaagaggat cttttatctc ctttgtccaa tctgaatttc
    69661 cgcaaaaaac aagtgtatat attaattctt tattatgggg ctataaagat gcaggattca
    69721 gagaaccaga agaaattttt agggagagtg gtttgctgca tctgttcagt ctatcgggtc
    69781 tacacatcca attttattta gggtggctgt attatttatt tcgacgtatc ggatggccac
    69841 tacacgtcag tattattccg ttgagtctac taactatctg ttatgtttct attgcaggaa
    69901 gttctatttc tgttttgcgt gctagtttgc tgtttttatt gcgattgcta gtcaaattgc
    69961 tgaataagca ttattccacc atggacttat ttgcagttgt cttttggctt cttttactat
    70021 ttgatcctcg atttcttttt catgcgggtg gacagcttag ttttttcatg agttttttgt
    70081 ttatcctgat ttcttttgat ggagattggt tgaaaaaaac ggcctctcac gtcctgttta
    70141 ctatgatcac gatgccgttg attgtatggc atttttatga gtggcctatt ttaggtagtc
    70201 tttttacatt actgattgca ccggtattca agtttctttt tcttcctggt gtgttatttc
    70261 tcacactctt caatcgctgg ctgccggaaa ctttactttt gctgatagaa gagggattaa
    70321 tcttatttga gcgaataatc gatttttctt cagggtcgtc tctcattata ggacgtttgc
    70381 cccttgtgct atctgggatg tatattttcg gattactgat ttgtatggat aggttgaaag
    70441 aacaacctgt gaaacacttg ttttatgttg gtcttttttc ttttattttt ttgactttgc
    70501 catttttacg cttttcttca acgatcgctt ttatcgatgt cggtcaagga gacagtatct
    70561 ttttgcaaag tccttttcga aaagaaacga ttttgatcga tactggaggt cagttacact
    70621 ttgaaagaga aaagtgggcg caaggtatga tacgtcctcc tgctgaaaga aatatcgttc
    70681 catatctaaa aggtcagggg ataagtgtaa tcgataaact catcttgaca cacgacgata
    70741 cggatcatgt gggagaatta cccacgttat ctcagcattt tactatcaaa aaaatttata
    70801 tcggttgggg cgctggacga aatgagccat tacaaaggca tttatctgaa gctcaaaaaa
    70861 atggtactga gatcattgaa atcaaacatg gggacaggat cagcggttat tatgatcttt
    70921 atgtattgac tccttttgaa aagggagagg gaagaaatga agattcgatt ggtctatgga
    70981 tggaatataa caataggcgc tttttatttt taggagattt gaatcaagca atggaaaaac
    71041 aattgctttt gatttatcct aatctgaaag ccgatgtagt caaattaggt catcacggta
    71101 gccgtacgtc aagtaacaca gattttcttt cccatattga agcaaaacat gggattatat
    71161 cctgcggcgt aaataatcgt tatggtcacc cgcacagcga agtattggag acactagaag
    71221 aaaatcagat aaaaacatgg cgaacggatc aacaaggaat gatcacctat cgctggcatc
    71281 cagtttttca tcctcatggg ctagtggaaa caatgataga ctagtaattc tcatctgctc
    71341 atggtaggat aatttttgaa agtggtgagt aatttgagta tacaagcaga gttacaaaaa
    71401 atacgtaaag aaaaacttgc atcttgttac ttgattttag gaacagaaaa gtttctccaa
    71461 gatcaagttc ggacagaaat tgtcaaaaaa atccaattag agggcgaaga tgacttaaat
    71521 tttttaagtt ttgacatgga aaatgcaagt ttagatgagg tagtagctga agcagagacg
    71581 ttgccgtttt tcggagatca acggttagta ttcgtagaga atccttattt tttaacaggt
    71641 gaaaaagtaa acaatggaat cgaacaaaat actgatttat tgacggctta tttgaaagaa
    71701 ccattagaat cgactgtatt agtattcttt gcgccatatg aaaagttgga tgaacggaaa
    71761 aaagtaacga aacaattgaa aaaaacagca attatcatca atgtccaaca gttgaatgaa
    71821 aaagaagtac gtcaatatct gatgaatacc ttagagaatt cctcaatcaa aatggaacga
    71881 agtgcaattg atttattctt gaggttgacc gacttagatt tatccaaaat gatgagagag
    71941 ttacagaaat tggtccttta tggacaaaat caacagcaaa tcacggtaag agaagtagaa
    72001 gaattagttc ctaaaacgtt agaacataat atttttgaca tgacccaata tattttaaaa
    72061 gggaagacgg aacaagcttt gcgtttatac gaagatttag ttatgcaagg ggaagaaaca
    72121 atcaaaatta atgcgatttt gctttctcaa ttacgtttat ttttacagac aaaatttctt
    72181 gtaaagattg gttatcagca ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt
    72241 gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac atttgctggt acagttgttt
    72301 gatcaattag ttgagatgga ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt
    72361 tttcaactat tcgttttgcg cacagggcag ttgttaagaa atcgataaga agaaagacga
    72421 gggcaaagca tgcgggaagg tcgtttgttt ttgtcctttt tttgacaaat aaaaaaatcg
    72481 gccgaagccg attcttttat ttagcgattt ttttgcttaa acgagatttg tcgcggttag
    72541 ctttgttttt gtggattaga ccttttgatt cagccatatc aatagcttta acagcttctt
    72601 tatataacgc atctacgttg tcagcacctg aagctacagc atcttcaaat tttttgattg
    72661 cagtacgcat agcgcttgtt tgagatgaat ttttcacatt agcgttttcg cttgtacgaa
    72721 cacgtttgat tgcagattca atgtttggca ttagtttcac ctccgataac ttaagttctt
    72781 acgcgtgtta ccgcataatt caacattcat tattatacct aaaggcagcg tgctttgcaa
    72841 tatgaacatg acaagttttt ttccttaaca gtcttttatt aggatttttt ttcagtaaaa
    72901 acggatagta caagctgaaa aaaatcaaaa agacgctatt gtgggaggca atagcgtcaa
    72961 accaaactag atttttattt atctatttaa acaaagtttg aatgggaatg tttggtacta
    73021 agttattata taacaaagag aaccgtttgc aaagtttttt ttgaaaaggg atacgattcc
    73081 ctgttgttct ttgtttttct attaagaaac tgcagttatt ttataaccag tatcctttga
    73141 tcagtaaagg ctattctggt gtaccgatta ttttaatgta ttcatcgata ttttttatca
    73201 acattttttt tatgaccaac tggttccgac ggttccattt gccacttact tccagcacat
    73261 atttatttag gggcaaacgt aagatatatt ttgccaaata gcgatcttta agcaggccat
    73321 tgatcacacg gtcctcaagc tgcaagttga tcccagtcag catcattggc tgttcttgta
    73381 aaacgtacgt atcaattaaa agaccttcaa aagtttccac aagcgtcttc ctttcttttc
    73441 aaagtaacct gaaaacaggc actaattctt ccattttgat attcgttgaa gtggatgcga
    73501 tcattctttc ttttgtccac tgtaatctag tataacaaaa aagagcagaa gagccaaaaa
    73561 gaaaaaagag catcgaattc tataggaagt ctcattggca cggcctaaat tattacaaaa
    73621 cattgattaa aaaaagcccc tgcatcgcag gagcatcccc tatatcgttt taataggtca
    73681 atcaaaaagc agtatcaaac acattttgta tcaaatcagg aaataataga taagctaagc
    73741 ccagaatcaa aagtaccgct agaagaaacc agccgattgt tcctatgaaa tactttaatt
    73801 tttcgaacat tgtatcacct cgttgtcagt atatcacgga tcagtaaatt gggggtgtta
    73861 agaatctcct aaaatagaca aaatttgttc tattttccta aagcaaatac ttgtaagata
    73921 cagtaagtga taccagaagc aatgaccggt ccggcggcga ttcctcttaa gaagacaaca
    73981 cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg gatcagaagc aatcaatcct
    74041 acacccttgg aagataagac agccactagc ccaccgcaaa aaacagctac ccagccaata
    74101 ggggatttaa aggcttctaa tagatcacgt agaccgattt ctcctgttgc gatagggatt
    74161 agaatagccg ctgtgatgat cgtcacccca atattgattc ctttgccttg aagccaaggg
    74221 aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa caaaagctgc cgctatcatc
    74281 agactttgat ttttagctaa aattgctaca agtaaaataa ttcctaaaaa aagccaactt
    74341 tccatgaaat atattcctca ctttcttcga aacaatcata acatgttttt agaaagttac
    74401 aaaagtagga attcatcctt caagacctat tcataagctg tttttttcat aaaataaaca
    74461 aaatcataaa aaaacacttt gcttatttga cagaagacag ccgcgctttg tatagtggac
    74521 aagacttaca tttttttgaa gaatcagaaa gatcaggtaa aggtcttagt aaacgcaagc
    74581 cattcagaat aaccaatatt gtgcttcctt cgtgtcctac aactccaaga ggtagattta
    74641 aaaattgcag gaagttagaa gcaatcaaca agagaataac aagaatcgaa aaaataatat
    74701 tttgtttgat gatccgccgc atttttaaag atagccgatg actgtagaca aatttattta
    74761 agtcgctttt cattaagacg atgtctgcga tatccatagc ggcatctgtc ccttgcccca
    74821 tagcgacacc taaagaagcg gctgctaaag caggcgcatc attgactcca tcaccaacca
    74881 tcgcatttac ttggtaaagt tcattctctt ttttgactag ttgtgttttt tcttccggag
    74941 tacagcttgc atagtaatgt tcaatcccta cttgttcagc aatggccttt gctgtgccag
    75001 aatggtcgcc ggttatcata cttgtatgga tgttttggga tttaaagtag gaaattactt
    75061 gttgggtatt tgcttttgga atatctagca gacctaaaac agcaattaac tgctgatctt
    75121 tagaaagata aatcacggtt ttgccttgat tttccaaacg ttcgatcatt tccgcaatct
    75181 ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt gccaactttc cattgcgcac
    75241 ctaaataggt agtttgtaat ccaaaaccag taatttcttc tattggtaag tattggattt
    75301 cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg agcaagtgga tgcgtcgttt
    75361 tttgttccat agcaacaaga agttgttgtg ccaaatcctt ttcatcaaag aaaaagctgt
    75421 cagttacaac tggttttcct ttagtgagcg ttcctgtttt atcaaaggca atcgctttta
    75481 attcagaaag cttttctaaa tggataccgc ctttgattaa aatcccattt tttgcagaat
    75541 ttgaaagagc agctagagaa gctggtgtag cagatgcgac aagcgcacaa ggagaagcca
    75601 caactaataa taccattcct cgataaaagc tttcatccca actccaacct aggaaaaagt
    75661 gggggagaag gatcattaga ggaacagcaa gcaaaatcaa ttttacataa ttattttcga
    75721 ttcgttcgat aaatgaagct gtttgagttg gcgtattctg cgcttcttct actaattgga
    75781 taatcttagc aaagacagtt tcggaactcg tttttgttac tcgaatcgtg atgggatttc
    75841 cttgattcag cgttcctgca tacatctcat cgcctaaata tttttctaca ggcacagatt
    75901 caccgcttaa tgcagactca tcgatcgttg cctgtgtgct ttctaaaaag ccatctatcg
    75961 gaacagctgc tcctttagcg acaaatacca agttatccac ctttagttga gaaacatcta
    76021 cttcttgcat ttgtccattt tcttttaata attgggcttt ttgtggttga agattcatca
    76081 aagcagtaat ttctttttga cttttattag ttgtgtattc ctctaatgca ccgcttaaac
    76141 aaaagatgaa tgtcaacatt gctccttcaa agtaattacc gatcaaacag gcaccaatag
    76201 cagctaaagc catcaataaa tccacattca gtgtccgatc attccacaat tctttcaagc
    76261 cttccgatgt ttgtttatat ccaccgatga taatggcact gatataaaaa agtggggtga
    76321 tggtaagaga gaatcggtcg aagataaaac cgaagatcat aaataaaagg cagccgattg
    76381 ttgctgcaaa agcttttttc tgattagtca tcgttattag acctccttgt caaaacaagg
    76441 atagcataat aaaaatgtaa ttgataataa taacagctaa atgagaacga taatggttat
    76501 catttggtga ttaagcttga tgttgttttt attatttata atgattatta ttaaagaaaa
    76561 taaaaaacca gaacatttct caaataagag aagtgttctg gttttttcta cattgcaata
    76621 aaccaataac caatcaaaat gataccaagg ataatacgat accagccaaa agcggtaaag
    76681 tcgtttttct tcaaatagct tagtaagaat ttgatagcta agacggaaac cacaaaagat
    76741 acgaatgttc ctacaagaag aacaacagtt tcttccattt tgaatgaatt cccttcagct
    76801 aaatatttga cgatcttcaa gccgcttgcg ccaaacatga caggaatccc taagaaaaat
    76861 gaaaactctg tagcgacaaa acgcgaagca ccaatcaaga tagatcctag gatcgttgcg
    76921 ccagaacggg atgttccggg aacaagtgcc aagacttgga ataacccaac aagaagagct
    76981 gcttggtacg taaagtcatt cagagaagtc caacgaggtg ttttgttttt gttgcgtttt
    77041 tcgacaataa taaaggcaat accgtaaacg atcaaaacaa tggcaactgg taagaacttg
    77101 tggaaatttg cttctaacca atcatccaaa ggcaaaccaa tgatgatggc tggtatgcaa
    77161 gcaacgatca ctttaaacca aagtgtccac gtatcttttt tttctaccgt tgatttactt
    77221 ggagaaaatg ggtttaactt gtgaaaataa agaacgacga cagccaagat cgcccctaat
    77281 tggataacga cattgaacat cgacataaat tgtgaactgg catccagttt gatgaattca
    77341 tctgctagga tcaaatgacc agtactactg atgggcaccc attccgttat tccttcaata
    77401 ataccgagaa tcactgcttt gataatattt gcaaaaaaca taaattcctt ccttcccaat
    77461 taagatacat cttatagtat accgttattt tttgagaaac ccaatctgtt ttttaatatt
    77521 aagaaaaact tgagaaataa agcagataag tacacgaaga gaaaaaagaa gacaaagccc
    77581 tcgttcagac tttgcctttt atgattctct ggtaaaaatt aaaatatccc cattatagaa
    77641 acgagtcgcg actatgtgtt ttcccgttat cgatataaga cgtatcattg atgccgtgga
    77701 tagaaaatcg accgtttgca taagtgacct ttgttacgct gccatttaaa agcccagcac
    77761 gaacaggctg tttaggatcg atcagattaa gcaaaaaggc gatcgtcaaa ccatgagata
    77821 cgactaaaac atttccacca ccattttttt cgcagtgatg ggcgatatct tcaaatcctg
    77881 tccatacacg gtctcgcaat gtttcataag tttccgccca cccagcggta tctaagttgt
    77941 atacagcatt agcaatttgt tcaaaagaaa cttctgtggt aaacatttca tcatatgttt
    78001 taaaattcaa tacacgaggg atcacacccc acatttccat gtcatatcca ccttcaagag
    78061 acccgaaaca ccattctcta atcctttttt caataaaata aggaatttct tttcccttag
    78121 aatgttctcc tagaataata cgggcagttt cgattgcacg accactgtca ctagaataag
    78181 caaatgaaaa gtcgacattt tttaacccgc gacctaaata atgaatgcct tcttgtcctt
    78241 gctttgtgag gggtgtatca caccagcctt gtactcgttc aattgtattg aacatcgttt
    78301 ttccgtgtcg tacgatataa agtgtagttt ctgtcatttt attctcctcc taagtcgttt
    78361 taacgattga aaaaaggatt tgtccttttt tcctgttcaa tcgtcgttgc atcgccatgt
    78421 cctggataga ctgcaaattc atcaggtaat acaaacagct gggtccgaat actatgaagt
    78481 aattgttcta aattccctgt atacagatcg gttctaccga tacttcctct aaacagggcg
    78541 tcgccagaaa cgacaaaatc atcaaaaata aagctgacgc ttcctataga atgccctggg
    78601 gtagggacga cgcgaaaaga catgtttcct aaagtataag ttttcatttc aaattcatat
    78661 tctgcaggcg agacaataac atcagcaata tcatcatgtc taccgagtcc agaaagattc
    78721 aaaatcggat cacctagcca tttttgctct agtgggctga cataaactgg gatttgataa
    78781 tacgcgcgta aaggttccac tgcaccgata tgatcgtagt gagtatgggt aagcaaaatc
    78841 gctaccggct gttggtttgt ttttttgatt tcttgtttga ttcgttccgc atcttctcca
    78901 gggtcaatga tcaaaagtgc ttcttcgttg tagaccaaat aacagttttc ttcaatcatt
    78961 cctgttttta atctttcgat atgcatgtgc attcctcctt gatagccaaa acttctttca
    79021 tccattagta taacgcattc attgctagga aacaaaaaca ggtacttatt tctctttact
    79081 tatctgaatt atcagactat atgaaaagta tttggatatt ctaaagagaa ggaaagtaag
    79141 ttttcatagc attttaatat gtccttgtta cattagattc atccaaaaat ccaaattcta
    79201 tatcctatat ccctgcctaa tcagcagggt gtttttttat gtttggaaag agagctcttt
    79261 ttgctaaact aggttttttc aggtaatctt agagagaaag aggtggtgga aatgatcaaa
    79321 cagtcaaaaa tatctgtgcg gcacttagta gaatttattt tgcgacgtgg aagtatcgac
    79381 aatcgtaaaa aaagtaacca tactgcatta gaaggagcga gaatccaccg caagctccaa
    79441 aaagatgcgg gagaaaacta tgaaaaagaa gtctttttaa aaacaacagt tcaattagat
    79501 gaatattacc tgatcgttga aggccgtgca gacggtattt tcaaaagaga cggtatttat
    79561 tatatagatg aaatcaaaac ctccgaacca cgatttgaag atttagaacc agaacaagtg
    79621 gaacttttct ttcatcaagc aagggtgtat gcctatatct atagtcaaga acatgattta
    79681 caagaaataa atctgcaatt gacttacttc cagacggcag aagaagtgat cacgagaaag
    79741 gttgaacatc agacaagaga gcagttagaa acttttttta aaaagctgac tgaagattat
    79801 aaagaatggc ttgtttttca agaaaactgg cgcactgtac gcaatgcctc tttgatggct
    79861 ttgaaattcc cccatgatga gtataggaaa ggtcagcgtg aactagcggt agctgcttat
    79921 aaaacgatcc gtacgaaaca gaaattgttt gtagaagcac ctaccggaac aggaaaaaca
    79981 atctctacgt tattccccgc tttgaaagct gtaggggaag aagagggaga aaagatcttt
    80041 tatctaacgg ctaaaacaat aactagacaa gtagctgaag atgctatgac agcattaaaa
    80101 gatacaggag ctgaagtaaa aagtgtcact ttgacggcga aagacaagat ctgcttttta
    80161 gatgagacga cctgtaatcc agatcagtgc ccatatgcca atggttatta caatcggatt
    80221 aatgaaggtt tatgggatct tttgaatcat gagaatcaga tcactcgtga agtcatcgaa
    80281 acttatgcaa gaaagcatac actttgtcct ttcgaactat cattagatgt cagtatctgg
    80341 tgtgacgtaa tcattgggga ttacaattat ttatttgatc caactgttta tttaagacgt
    80401 ttttttgaag atgaaaaaaa cgaggactat ctgtttttga ttgacgaagc acataatttg
    80461 gtgaatcgtt cccgagagat gtattcggca gaactatctt atgaaaaaac gaaacgaagt
    80521 aaggaagcta taccaaagga attcaaaaaa cttcatcgcc gtttcaacaa actattaaaa
    80581 gaatttgatt cgatccgaga aatagcaaaa gaagatcatt gggattatca tcatcaaaaa
    80641 gcaccggctg aatctttagt aaaggcaggt tatcaattaa gcgaaaaaat taaagaatgg
    80701 ctggcagaat ttcctgagca cccatcacaa gaacaattgc ttccttatta ttttgatttg
    80761 cttcattttt tgaaggtcag tgaattttat gatgatcact acgaaacaac agtcgaaaaa
    80821 acgtatcgcg atctgatagt aaaagagttt tgtattgatc caagcttgtt tcttgagcag
    80881 tcgttggata aaggaaaaag cagtattctt ttttctgcta gtttttcacc actctcttat
    80941 tatcaggaaa cactgggggg acaaaaaagt ctggcttaca aactgccgag tcctttccca
    81001 gaaggaaacc agcaagtttt catcgcaaat tacctagaga cgacttatcg aaaaagggaa
    81061 cacagtctgg caaaattagt agagacgatt catgcttttg ctgatggaaa ggtcgggaat
    81121 tatttcgttt tctttccttc ttatcaatat ttagatcttg cggtggaagc attcagacag
    81181 caatatcccg acagcaatgt gctaatccaa gaaacggaca tgaatgagga agaacgcgaa
    81241 cgatttttaa caaaatttca aacaaatccc gctgaaacat tgattggatt ttgcgtactt
    81301 ggcgggattt tttcagaagg aatcgatttg agaggggacc gattgatcgg cagtatgatc
    81361 gtaggtgtag gtttgccgca gatgaatcat gagcaggaac tgatcaaatc atattatgat
    81421 gagaaggaac accttggatt tgcttacgct tatcagcttc ctggaatgaa caaagtcttg
    81481 caagctggtg gtcgagtgat tcgtggcatg gcggatcaag gaatcgttgt attggcagat
    81541 caacgatttt ctagtttttc ttatcgacgt ttattcccac aacattggca aacagcaaga
    81601 gaagtacgaa gtgttgggga attggaagaa ggaatacagc gtttttggtt atcaaaagaa
    81661 gcagtaaatc aaaaaaacga ggaatgacag acgtcagcct cgttttttga ttaacgatag
    81721 ttagtgaatt gcaactcgat tggtaagtca agtttacgta ataaagcaat cacagcttgt
    81781 agatcatcac ggttcttacc tgtcactcta atctgatctt cttggatttg tgcttttact
    81841 ttcagttttt catttttgat agctgtagtg attttcttcg cgttttcacg atcgattccg
    81901 ttgaccaaat cggcagtttg tcgagctttg ccacctagag cgtgttctga ttcggaaaaa
    81961 tggatatttt tgattggcac atttcttttg atcagcttac caaataatac atctttgatc
    82021 tgttcgattt tgaaatcatc atcgctcaat acaactagat tgctgttctc taatttgatt
    82081 tctactgtag aatccttgaa gtcaaagcga ttggacaatt cttttgttgt ttgctgaatc
    82141 gcatttttta cttcttctgt attgacttct gatacaatgt cgaagctagc atcttttgcc
    82201 ataggaaccc tcctgatata catataaata tttaccatac atctatctta tcataggatt
    82261 agggcagatt cttacctgtt tgctaaaatt cttctcattt gagttcgaag tattttattt
    82321 taatagataa actgttattc ttacatttgg aataccaaag taggggagag gtgcttgtga
    82381 gtaaaattat gactgcgtat cataaaaatg aaagattaaa gaaaatagtg gttattttga
    82441 taaccggatt gaccgctgcg gtcgctttaa attatttttt gattccagcc aatgtctttt
    82501 ctgcagggat gaatgggatc gctcaaatca tttcctcgct tttatatgaa tggtttcata
    82561 tcaagataga cacaggaaca tttatttttc ttttgaatat ccctgtgttt gtattagggt
    82621 ttctcaaagt aggaaaacga gcaacgatcc ttagttttgt gaatgttgta tgtgtatcgg
    82681 tgatgacgat gctgcttcca gtcggacaga tcactgataa tattttgatg aatgccctag
    82741 taggcggtgt tttattaggt cttggcgtag gattatcttt aaaaatgggc tttacaactg
    82801 gtgggatgga tatcatctct cttgtacttt cacaaacaac tggaaaaaca gtcggaaact
    82861 acatgctgat tctaaatggt atcattgtag tcatcgcagg cttcttcttt aactgggaaa
    82921 gtgccttgta tacgattatt tccatttatg caatgactca agtaattgat gccatccaca
    82981 cgagtcatca aaaagtaaca gcaatgatcg ttaccgtcaa gccagaagag gtaacagaag
    83041 aactagcaaa acggatggta cgtggaatga cattactgcc gtctgtcggt ggctattcaa
    83101 aaaaacaagg gaacatgatc atgatggtca tcacccgcta cgaaatgtat gatcttgatc
    83161 agatcgttca tgaaatcgac gacgatgcat ttatcaatat tttaccaact cattcagtat
    83221 ttggccggtt tgcaaatgaa aacgaacaaa aaatgtatcg aacaactggc gtattgccag
    83281 aaataaaaaa tcgttcacaa aaaaaatgag gaatcaggtt tattttagaa taaatttaat
    83341 ctattacatc taataagaac atttttataa atttaaacga gaatcttact tctaaattct
    83401 catttacaag cagctaaaaa aagagtatga taaataaaaa tcgtgttaag gagtgtcgta
    83461 cgatgagcga agcaaaccaa gaacggtctg gacaagaaat agaactaatc aaagaacgta
    83521 ttcttgctgc cttggaaatg gtgattgatc ctgaattagg aatcgacatc gtcaatttag
    83581 gcttgattta tgatatagaa ttcaatcctg aaaatggaga aacagtcatt aagatgactc
    83641 taacaactat gggctgtcca ttagcagata ttttaacaga atctatccat gacgcattaa
    83701 aagaagtgcc agaagtaacg aaagcagaag tcaaactagt ctggtatcca gcatggacga
    83761 cagataagat gagccgttat gcacggattg cgttggggat acgatagaaa tcttggctga
    83821 ctagctatga gaagcaaata ggaaaaacaa aaaagccttt tatcctttct agaagaaaag
    83881 agataaaagg tttctttgtt tataaaattt tgtctgctat attataagtt aattaa
//
LOCUS       DAYYHB010000002        66090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000002 DAYYHB010000000
VERSION     DAYYHB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.002"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..401)
                     /locus_tag="ACHA6B_000080"
     CDS             complement(102..401)
                     /locus_tag="ACHA6B_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8947692.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            752..1384
                     /locus_tag="ACHA6B_000081"
     CDS             752..1384
                     /locus_tag="ACHA6B_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8947693.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1507..2145
                     /locus_tag="ACHA6B_000082"
     CDS             1507..2145
                     /locus_tag="ACHA6B_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8947694.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2264..2336)
                     /locus_tag="ACHA6B_000083"
     tRNA            complement(2264..2336)
                     /locus_tag="ACHA6B_000083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2301..2303),aa:Thr,seq:cgt)
     gene            2457..2699
                     /locus_tag="ACHA6B_000084"
     CDS             2457..2699
                     /locus_tag="ACHA6B_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947695.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2838..3467)
                     /gene="upp"
                     /locus_tag="ACHA6B_000085"
     CDS             complement(2838..3467)
                     /gene="upp"
                     /locus_tag="ACHA6B_000085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8947696.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3601..4548)
                     /locus_tag="ACHA6B_000086"
     CDS             complement(3601..4548)
                     /locus_tag="ACHA6B_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8947697.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4701..5945)
                     /gene="glyA"
                     /locus_tag="ACHA6B_000087"
     CDS             complement(4701..5945)
                     /gene="glyA"
                     /locus_tag="ACHA6B_000087"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8947698.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6120..7145)
                     /locus_tag="ACHA6B_000088"
     CDS             complement(6120..7145)
                     /locus_tag="ACHA6B_000088"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8947699.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7167..7346)
                     /locus_tag="ACHA6B_000089"
     CDS             complement(7167..7346)
                     /locus_tag="ACHA6B_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947700.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7339..8178)
                     /gene="prmC"
                     /locus_tag="ACHA6B_000090"
     CDS             complement(7339..8178)
                     /gene="prmC"
                     /locus_tag="ACHA6B_000090"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8947701.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8171..9244)
                     /gene="prfA"
                     /locus_tag="ACHA6B_000091"
     CDS             complement(8171..9244)
                     /gene="prfA"
                     /locus_tag="ACHA6B_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8947702.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9247..9831)
                     /locus_tag="ACHA6B_000092"
     CDS             complement(9247..9831)
                     /locus_tag="ACHA6B_000092"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8947703.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9937..10185)
                     /locus_tag="ACHA6B_000093"
     CDS             complement(9937..10185)
                     /locus_tag="ACHA6B_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8947704.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(10176..10406)
                     /locus_tag="ACHA6B_000094"
     CDS             complement(10176..10406)
                     /locus_tag="ACHA6B_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947705.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            10512..11114
                     /locus_tag="ACHA6B_000095"
     CDS             10512..11114
                     /locus_tag="ACHA6B_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8947706.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11218..11787)
                     /gene="lepB"
                     /locus_tag="ACHA6B_000096"
     CDS             complement(11218..11787)
                     /gene="lepB"
                     /locus_tag="ACHA6B_000096"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8947707.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12017..12784)
                     /locus_tag="ACHA6B_000097"
     CDS             complement(12017..12784)
                     /locus_tag="ACHA6B_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8947708.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12781..13260)
                     /locus_tag="ACHA6B_000098"
     CDS             complement(12781..13260)
                     /locus_tag="ACHA6B_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8947709.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13385..14122)
                     /locus_tag="ACHA6B_000099"
     CDS             complement(13385..14122)
                     /locus_tag="ACHA6B_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8947710.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14283..15683
                     /locus_tag="ACHA6B_000100"
     CDS             14283..15683
                     /locus_tag="ACHA6B_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8947711.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15906..17258
                     /locus_tag="ACHA6B_000101"
     CDS             15906..17258
                     /locus_tag="ACHA6B_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8947712.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17251..17949
                     /locus_tag="ACHA6B_000102"
     CDS             17251..17949
                     /locus_tag="ACHA6B_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8947713.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18011..19162)
                     /locus_tag="ACHA6B_000103"
     CDS             complement(18011..19162)
                     /locus_tag="ACHA6B_000103"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8947714.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19175..19612)
                     /locus_tag="ACHA6B_000104"
     CDS             complement(19175..19612)
                     /locus_tag="ACHA6B_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8947715.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19627..21393)
                     /locus_tag="ACHA6B_000105"
     CDS             complement(19627..21393)
                     /locus_tag="ACHA6B_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8947716.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21657..22334
                     /locus_tag="ACHA6B_000106"
     CDS             21657..22334
                     /locus_tag="ACHA6B_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8947717.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22337..23464
                     /locus_tag="ACHA6B_000107"
     CDS             22337..23464
                     /locus_tag="ACHA6B_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8947718.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23664..24104)
                     /locus_tag="ACHA6B_000108"
     CDS             complement(23664..24104)
                     /locus_tag="ACHA6B_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8947719.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24115..26148)
                     /locus_tag="ACHA6B_000109"
     CDS             complement(24115..26148)
                     /locus_tag="ACHA6B_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8947720.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26150..27853)
                     /locus_tag="ACHA6B_000110"
     CDS             complement(26150..27853)
                     /locus_tag="ACHA6B_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8947721.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27947..28897)
                     /gene="manA"
                     /locus_tag="ACHA6B_000111"
     CDS             complement(27947..28897)
                     /gene="manA"
                     /locus_tag="ACHA6B_000111"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8947722.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28936..29961)
                     /locus_tag="ACHA6B_000112"
     CDS             complement(28936..29961)
                     /locus_tag="ACHA6B_000112"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8947723.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30170..31039)
                     /locus_tag="ACHA6B_000113"
     CDS             complement(30170..31039)
                     /locus_tag="ACHA6B_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8947724.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31743..33635)
                     /locus_tag="ACHA6B_000114"
     CDS             complement(31743..33635)
                     /locus_tag="ACHA6B_000114"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8947725.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33632..35359)
                     /locus_tag="ACHA6B_000115"
     CDS             complement(33632..35359)
                     /locus_tag="ACHA6B_000115"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8947726.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35375..36025)
                     /locus_tag="ACHA6B_000116"
     CDS             complement(35375..36025)
                     /locus_tag="ACHA6B_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8947727.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36229..36525
                     /locus_tag="ACHA6B_000117"
     CDS             36229..36525
                     /locus_tag="ACHA6B_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8947728.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36717..37337
                     /locus_tag="ACHA6B_000118"
     CDS             36717..37337
                     /locus_tag="ACHA6B_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8947729.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37429..38595)
                     /locus_tag="ACHA6B_000119"
     CDS             complement(37429..38595)
                     /locus_tag="ACHA6B_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8947730.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38607..38960)
                     /locus_tag="ACHA6B_000120"
     CDS             complement(38607..38960)
                     /locus_tag="ACHA6B_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8947731.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39088..40110
                     /locus_tag="ACHA6B_000121"
     CDS             39088..40110
                     /locus_tag="ACHA6B_000121"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8947732.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40129..40290
                     /locus_tag="ACHA6B_000122"
     CDS             40129..40290
                     /locus_tag="ACHA6B_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947733.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40549..40983)
                     /locus_tag="ACHA6B_000123"
     CDS             complement(40549..40983)
                     /locus_tag="ACHA6B_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947734.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41093..42952)
                     /locus_tag="ACHA6B_000124"
     CDS             complement(41093..42952)
                     /locus_tag="ACHA6B_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8947735.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            42953..43093
                     /locus_tag="ACHA6B_000125"
     CDS             42953..43093
                     /locus_tag="ACHA6B_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8947736.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(43070..44229)
                     /locus_tag="ACHA6B_000126"
                     /pseudo
     CDS             complement(43070..44229)
                     /locus_tag="ACHA6B_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(44536..45960)
                     /gene="araA"
                     /locus_tag="ACHA6B_000127"
     CDS             complement(44536..45960)
                     /gene="araA"
                     /locus_tag="ACHA6B_000127"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8947737.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45976..46674)
                     /locus_tag="ACHA6B_000128"
     CDS             complement(45976..46674)
                     /locus_tag="ACHA6B_000128"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8947738.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46631..48292)
                     /locus_tag="ACHA6B_000129"
     CDS             complement(46631..48292)
                     /locus_tag="ACHA6B_000129"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8947739.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48510..49910
                     /locus_tag="ACHA6B_000130"
     CDS             48510..49910
                     /locus_tag="ACHA6B_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8947740.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50162..51241)
                     /locus_tag="ACHA6B_000131"
     CDS             complement(50162..51241)
                     /locus_tag="ACHA6B_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8947741.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51327..52286)
                     /locus_tag="ACHA6B_000132"
     CDS             complement(51327..52286)
                     /locus_tag="ACHA6B_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8947742.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52298..53809)
                     /locus_tag="ACHA6B_000133"
     CDS             complement(52298..53809)
                     /locus_tag="ACHA6B_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8947743.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53831..54394)
                     /locus_tag="ACHA6B_000134"
     CDS             complement(53831..54394)
                     /locus_tag="ACHA6B_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8947744.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54400..55263)
                     /locus_tag="ACHA6B_000135"
     CDS             complement(54400..55263)
                     /locus_tag="ACHA6B_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947745.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55277..56173)
                     /locus_tag="ACHA6B_000136"
     CDS             complement(55277..56173)
                     /locus_tag="ACHA6B_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947746.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56348..57637)
                     /locus_tag="ACHA6B_000137"
     CDS             complement(56348..57637)
                     /locus_tag="ACHA6B_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8947747.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57609..59126)
                     /locus_tag="ACHA6B_000138"
     CDS             complement(57609..59126)
                     /locus_tag="ACHA6B_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8947748.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59324..60214
                     /locus_tag="ACHA6B_000139"
     CDS             59324..60214
                     /locus_tag="ACHA6B_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8947749.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60287..61111)
                     /gene="nadE"
                     /locus_tag="ACHA6B_000140"
     CDS             complement(60287..61111)
                     /gene="nadE"
                     /locus_tag="ACHA6B_000140"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8947750.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61123..62595)
                     /locus_tag="ACHA6B_000141"
     CDS             complement(61123..62595)
                     /locus_tag="ACHA6B_000141"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8947751.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63232..63594)
                     /locus_tag="ACHA6B_000142"
     CDS             complement(63232..63594)
                     /locus_tag="ACHA6B_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8947752.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63817..64569
                     /locus_tag="ACHA6B_000143"
     CDS             63817..64569
                     /locus_tag="ACHA6B_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8947753.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64709..65632)
                     /locus_tag="ACHA6B_000144"
     CDS             complement(64709..65632)
                     /locus_tag="ACHA6B_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8947754.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65647..65919)
                     /locus_tag="ACHA6B_000145"
     CDS             complement(65647..65919)
                     /locus_tag="ACHA6B_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947755.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19123 a        13536 c        11476 g        21955 t
ORIGIN      
        1 atttagaata aaaaaataca gactctgttc aaataaatga acctagcctg cattatgaga
       61 ttgactatac tttttaattc attgagatga ataactcgtt tttataaatg cccaaaagta
      121 atcggtgccg aagccattcc tcgacctaat tctttttcaa attttcctat tgttgatatc
      181 tccatataaa acggtgatac tggtttagac aattctcccc tgattgctaa cggacgtaaa
      241 ctacgttcca aattatttat gactttagga taatactgac ctttttctaa gcctgtcttt
      301 gcgtctatca aaagtatacg ttccttttct ttgatctctg gatttaaaac gagatcatat
      361 aattttagta ccatgttttc tatttcttca cttttagaca tcacgatttc ctcccaaaaa
      421 tatatttctt cccactacat tctataaaca aagtatacgc tacatctaga tttttttaaa
      481 tcattactgg attgttaaaa cctttcaata cactgttatg gcaacgattt ttattttaaa
      541 aacattctaa attaaatcag taaaaatttt tattctaaga tttttattag aattacgcct
      601 aatttgtgtc tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat
      661 cgaaaaaggc gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa
      721 tatgacttta tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag
      781 cttgaataaa ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat
      841 cctttcacaa aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc
      901 tattttcaag tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg
      961 tatattcgaa aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt
     1021 gaaatttgtc aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac
     1081 gttattcgag aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag
     1141 tacctttgag cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat
     1201 aatgttttct actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat
     1261 cgatttaagt agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg
     1321 tacgcttaat cagttgcaag cacttggttt tgctagaaaa tttttacaaa taaaagtagt
     1381 ataatcaaaa tgaatatata agataaattt ttagcaatac aaatatccat aaaaaaacat
     1441 tgcctatttt aactcttcat gtatactgac tctatcttca aaaactgaat agtaggagtt
     1501 attatcatga ataacatttt aggaaaaaaa atagaactta aaaaagggat attgggcact
     1561 attgtcgttg tactcggttt gttagggttg ctatttgcgc catccattat tagcattttt
     1621 tctttaacta tcactggatt tatagcattt acacttgttt ttggtaaaaa agatacgaaa
     1681 aaaatgttct cgaaacctgt cgctcctgtc aaaaatatag ccaaatattt ttttatcaat
     1741 gtagtaatca gtgccgcagt ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca
     1801 ggcaatccaa taaatgaggc tttcagcccg ttattattta ttattttgcc tatcatgata
     1861 cttggagaag aattgttctc agtgtatttt ctcgctattt tttctagtaa gttcagcatt
     1921 cctgtcgcta gtttcttgtc agcaatcatc tttggattta tccattacag tacttatgat
     1981 aatggaaata ttttacacac tgtagcacac atccttttga tacaaggggt ggcaagattg
     2041 ttattcaacc aagctgccat taaaagcaac tcgatcatta caagttgggc agttcatgtg
     2101 atatttgatt tcacagctat attgctagtc gttttattct cctaaatttt tatagaaata
     2161 atacatgctc tattgctaag aaagcatact ttatttcctt taaacaaaaa gacagatgta
     2221 tcaatggatt aaaaatcctt gatatatctg tcttctcttt taatgccagc tatagggatc
     2281 gaacctacga cctacgcgtt acgagtgcgt tgctctacca actgagctaa gctggcctta
     2341 tccaaaagaa attatatact ctatttggct gattgtaaag agtccgatgg aaaattagtt
     2401 taatttcatg atatttttca taaaacgtgg tataattgat gtgaaaggca ggttttatga
     2461 aaatggcaac ttttggaaaa tttgatacaa ctatcgtacc atcggaaatt ggtgaaaaat
     2521 tcaagatcaa tactcacgta atgttcacaa ttcacggcaa gtcgcaaact ggaacgattt
     2581 caaagcaatt gaaaaattcc gctgttgttg agattgatga aactgcagaa aatgcgaact
     2641 tgatgacgca aagtaagggt gtagtaatcg ttaactacaa agaactaaaa aagatttgaa
     2701 tttgaccatc tctgcatttt tgggatggtt tttttatatg caaacgaaag ctgtgaaaaa
     2761 gctgttctgc atcaaacgtt aaaaatgacc aaaaaaggat gtgacaaaag ttgtgtcaca
     2821 ccctttttcg tatttactta ttttgtacca aacaaacggt cacccgcatc ccctagtcca
     2881 ggaacgatat aaccatgttc attcaatttt tcatctagag aagctgtata gatgtcaata
     2941 tcaggatgcg cttcttgcaa agcttttact ccctcaggtg ctgctaccaa acaaacaaat
     3001 ttaatgttcg acgcgccacg ttctttcaat gcatcgattg ccatgattgc tgatccgcct
     3061 gttgccaaca ttggatctac tacaaacaac tgacgtgcat caatgtcttc tggaagtttg
     3121 acgaaatatt catgtggttc caatgtttct tcatctcgat aaagaccgat atgtcccact
     3181 ttagcagctg gaatcaattc caagataccg tcaaccattc caatacctgc gcgcaagatt
     3241 ggaataatcg ctactttttt accagacaac gttttttgtg ttgtttcagt gatcggtgtt
     3301 tcgatcacga tgtcttccaa tggcatatcc cgtgatactt catatgccat caacatagcg
     3361 atttcatcga ctacttcacg aaaaaccttt gttccgcagt ttttgtcccg aataatagtt
     3421 agtttgtgtt gaatcaatgg atgatcgatc acttgaaatt tgcccatttt ttaagctctc
     3481 ctttgttctg tcgtttcttt ttaattgtaa aacaaaatca agtgattgac tagtcaaact
     3541 cgccattttt cctaagaaaa tccgtttttg aaaagaagtt ttcctgcgga caagcaaagc
     3601 ttattctttt ttcttgatcc aagaagtaga taatgccatt gctgcacgcg ttgcctttgt
     3661 ctgatccata gcatttacca tgacaaaatc atggatgatt ccttggaagc gtacttgtgt
     3721 aacaggaacc cctgcatcac gaagtttgcg cgcataagcc tcaccttcat ctcttaatac
     3781 atccgcttct cccgttaaaa tcattgctga aggaagatca tttagttcat ttatatttgc
     3841 tctcaaaggc gaagctgtga tttcagcacg ctcttcttca ttcgtcgtat attggtccca
     3901 aaaccacatc attccttctc ttgtcaaaaa atagttttct ccgaattcat gataggaatc
     3961 tgtatcaaaa tcagcgtttg tgactggata atacagcaat tgcttggata ttgggaggcc
     4021 ctttcgttct tttgttaaga tcgtcatgac tgtagccatg ttaccaccta ctgaatctcc
     4081 tgcaacgatt agattctcga gatccagatt ttttgacttg gctaatttat tcagctgttg
     4141 caacacagca taattttgtt caattgctgt tggatatttt gcttctggtg atcgactata
     4201 ttctggaaag acaacgacgg aattcgtacg aaccgcaagt tctctgatca atttatcatg
     4261 ggtttgagca ctgccaaaaa cccagcctgc tccatgaata taaaaaataa ctggtaattt
     4321 atccatattc ccttctggtc gtacgagacg aatattgagt tttccccatt cgccagtatc
     4381 caccatcaag tcttcaatat cgacggaata tttgttcaca ggtgattctt gtacttcttc
     4441 tagtaaaact cttccttctt ccggtgctag ttcatagata cgcggatgag gcgcatttgc
     4501 atcactgaat gctactgctt ccttttctaa cgagatacga tcagtcatga cttttcctcc
     4561 ctgagattta gtgtgaagac taaaattaca cctcatatac tagagagcat caccgatatt
     4621 gtcaaagaat ccgttttccc aaaaaataaa cagaaaagcc caacattgaa cttttctgtt
     4681 tactaatgga atttaaattt ttatttttta tgcaacggaa atttttcagt taattcgcgt
     4741 acacctgttt tgacttcgtc tagttgagcg ttatcatctt ttgcttgtaa tgctttgaca
     4801 actagttcag ccacttttac agcatcttct tctttgaacc cgcgagtagt gattgctggc
     4861 gtgccgattc gaataccgct tgttttgaat ggacttagtg tttcaaaagg aatcgagttt
     4921 ttatttacag tgatattcac actgtccaaa atgctctctg cttcttttcc atttatacct
     4981 aactcgcgaa catcgatcaa catcagatga ttatctgtcg cccctgaaat gactctggtt
     5041 ccaattgctt gattgaaaac tttgaccatt gcttttgcat tggcgatgat ttgttcacta
     5101 tattctttaa atgcaggatc taatgcttct ttgaatgcta ctgctttacc cgcaatcaca
     5161 tgctctagag ggccgccttg aataccagga aataccgcac tatttatttt ttttgctaat
     5221 gcttcatcgt ttgtcaaaat catgccgcct cgagggccac gcaatgtttt atgagtggta
     5281 gtcgttgtga tatctgcata aggaactgga ttaggatgaa gtcctgctgc tactaatcct
     5341 gcgatatgtg ccatatctac catcagtttt gcgcctactt catctgcgat ttctctgaat
     5401 ttcgcaaagt cgatcgttcg tccgtaagca ctggcacctg ctacgatcaa ttttggttga
     5461 tgttttctag ctaaaatacg taccacatta taatcaataa cttctgttgt cggatcaaca
     5521 ccgtaagcaa caaagtgata ggttttgcca ctgaagttga ctggtgatcc atgtgttaga
     5581 tgtccgcctg cggaaagatc cattcctaag atcgtgtccc ctggttctac caaagcgaga
     5641 taagcagccg tgttggcttg agagccggag tgaggctgga cgttcgcaaa tttcgctccg
     5701 aataattctt ttgcacggtc aatcgctaaa ttttcaacga tatcgacaaa ttcacaccca
     5761 ccataatagc gatgccctgg ataaccttct gcgtatttat ttgtcaagat acttccttgt
     5821 gcagccatca ctgcttcgga gacaaagttt tcagaggcaa tcagctccaa attatgttct
     5881 tgtctttctt cttctttggc gatcgcagcc caaagatcag ggtcaaacgt tttgtaatct
     5941 accacaaaca acagctcctt tttattttcc tacaatttta tttttcccat caaaaacaaa
     6001 ttgccaaata ttcgtatctc ttctattacg attagttacg cttagttttt cgttcactga
     6061 tagaagattt acttgatagc attgtatcat actaaaagaa tgcgacaata accaattgat
     6121 catttttcaa aataattttg tcctgctgct tttttcaagc gattcatgta agcattcccc
     6181 aatccagttt ctggatacac ttgtacaaaa atcaaatcta atccaagtgt tggttcatct
     6241 aatcctctta gacctgcaaa caatccttta gccgccgctt ctactgagtt gtcgttatac
     6301 atataaacag ctgcagtatc tgtccgcact tgatccgcaa tttcggggct tgctatgaca
     6361 cctgcacgga tcttcttatt ttttgcccat tgaactgcag ttgaccaatc gccatctctg
     6421 accatcagaa cacgtgtatc gggtgagtag tgtttgtatt tcatcccagg ggcttttggt
     6481 gtttcatttt cttttacaag atgctgatca atagcaacag gagattcgat gactgcttcg
     6541 atttgttctt tcgttactgc acctggtcgt aaaatcatag gcatggctgt tggatcactt
     6601 aaatcaagaa cagtggattc tacaccaatt cgcgtagctc catcatcaat aatcccggta
     6661 atttttcctt gtagatcatg atatacatga tctgcagtcg ttggacttgg ttttcccgac
     6721 gtatttgcac ttggaccgac aagcggaaca ccggaaagtt cgattacttc tagtgttttt
     6781 ttgttatccg gtatacgaaa tgcagctgtg gataatcctc ccgtcacaac tgagggcaaa
     6841 gtgtcttttt tgatcttaaa tatcaaagtt aatggtccag gccagaagtt cttcacaatc
     6901 ttctctgctg ccggatgaaa gttgtccaca tactctttta cttgttcaaa agaggcgaca
     6961 tggacgatta atggattatc ttgcggtctc ccttttacag aaaacacttt cttgacagcg
     7021 tttggtaaaa gagcgttggc gcccaatccg tatactgttt cggtaggaaa agcaacaagt
     7081 tctccttctt tcagtgcttc ggctgcctct tgcaattctg tttcatcata tcgtttcgtt
     7141 tccacagttt atccacaacc cctttttcat acattcttat tttgtctgag tttatcctca
     7201 agttttccac tgtttgtgga taatctattt gtcaatagtg gataaacttg ttgataaacc
     7261 aaaaagtcgt ttacatcctt gtcaaataag tgttttttta taataaaaaa acaaataaaa
     7321 aatttttcat ccacaacttc aaccataaac ttggatcatt cgttcattgc caaaaagatc
     7381 cttttttatc gccacttttt tatctggaaa tgcttgctga aatatttttt ttactgcttc
     7441 accttggcga aatccgattt ctaaaaaaat ctttccattt gattttagta cgcttggtgc
     7501 ttcaactgct attttttcat aaatagctaa tccatcgttc tcagcaaaaa gggccatttt
     7561 aggttcgaat gaccggacac tttcatccat cagttcccat tcattccgac tgatgtacgg
     7621 cggattagaa ataatcacat caaattgctg atccattact ggtcctaatg tatctccatg
     7681 ataaaaactg attttcgttc cttctttctg agcattttct ttcgctactt ctaatgcttc
     7741 ttctgaaaga tccacagcag atatatgcca attttttctt gctgctttca aactaatagc
     7801 aattgctcca gtccctgtcc caatatctat cacttctaac ggtacaccag gcgtctcgtc
     7861 taaacaccat tcgactaact cctctgtttc tggtcgaggg atcagtgttg cttcagttac
     7921 ctttaaccga tgatcataaa aatctgcata tcctagcaaa tactgaggtg gatgatctgc
     7981 aagcagaagt cgcagatcgg tttcgatcaa ccgctgttct tcttctgaaa tctcttcgtt
     8041 catatgaagg agccaatcaa gttttttcca ttgttttcgt tccaaaaata aaaattggat
     8101 actatatgct tctttcccat gatcctctaa aaaagaagaa gcccgtgaca ggacttctcg
     8161 ataggtcatt ttacccattt tgcatagctt ccagttttga tgtttgatca tacatgatca
     8221 atgcatcaac gatctcatct aatttccctg ctaaaatctg atctaatttt tggatagtta
     8281 atccgatacg atgatcagtc acacgatttt gcgggaaatt atacgtccgg atccgttctg
     8341 aacgatcccc agttccgact gctgattttc gattggcatc atactcgctt tgtgcttctt
     8401 gctggatctt atcgtacaca cgagcacgca agattttcat tgctttttca cggttcttga
     8461 tttgagaacg ttcatcttgc atagcaacaa cgatccctgt tggtaagtga gtcaaacgta
     8521 cagcagatgc tgttttattg acgtgctgtc cgccagcacc tgaagcatga tagatatctg
     8581 tacggatatc tttgtcttca atatcgatct ctacttcttc agcttctggc atcacgacaa
     8641 ctgttgcggt ggaagtatgg atacgccctt gcgattcagt tgaaggaaca cgctggacgc
     8701 ggtgcgcacc actttcatat tttaatttcg aatatacgtt gtctccggtg atcatcatga
     8761 tcacttcttt atatccaccg attccggtaa cgttagcttc catcacttct gttttccagc
     8821 cttgtgcttc agcatacttt tggtacatat tgaaaagatc gccagcgaat aatgctgctt
     8881 cgtctccgcc tgctgctcca cggatttcca tgatgatatt tttatcgtca tttggatctt
     8941 ttggtaaaag caagatcttg attttttctt ctaagacttc tttttctttt tttaattcag
     9001 ataattcttc ttttgctagt tcagccattt catcatccag tttttcacca agcagttctt
     9061 ctgtatcggt gattccttgt actacttctt tataacgacg ataaacttcg actgtttcgc
     9121 gcgtgtttgc ttcttctttt gaaagctgca taaaacgctt tgtatcagaa atcacttctg
     9181 ggtcacttaa aagttctcca agctcttcat aacggtcttc aattgcttgt aattgatcgt
     9241 acatgcttat tctccttctg taatcattga tgggtgaaag taatgttttc gacaaacggg
     9301 gtaataagct tcatttccgc caatttgcac ttgatcgcct tcataaacag ggtttccatc
     9361 aatataatgt aaattcatga ttgctttttt gtggcagaac caacagattg ttttcatttc
     9421 ttcgattttg tctgcataaa gcaataaata ttttgatcct tcaaataact cgtttcgaaa
     9481 atcatttttc aaaccaaatg ccataaccgg aatatccaat tcgtccacaa ttctcgtaaa
     9541 agcaatgaca tgtttctttt ttaagaattg cgcttcatcg atcagcacac aatagggttt
     9601 gctttccatt ttctgaatta cgtcaaatac gtctgtttct tcaaatatcg gcatcgcatt
     9661 acgtttcagt ccgattcggc ttgaaacaac gcctaaacca tctcttgtat caataccgct
     9721 tgtcattaag acaactggct tattttgttc ttcataatta tgtgcgactt ttaggatctc
     9781 gatcgtcttg ccgctattca ttgcgccata cttaaaaaat agttgtgcca tgctcttttt
     9841 caccttccag tttgttccta tggtattata cttgattttc agcatttttt acagtgagaa
     9901 ttttcatcaa gcacagatta gtattaaatc tattgctcac ttttgttcca attttttgac
     9961 aagaactaaa catgggttcc attcatccca taaatttgga aaaacttcca accttgaaaa
    10021 tcctaatgat tcgtagaaac aaatagtttg atcatatata gagtagtgcc cctcgtctac
    10081 agtcttcact tgcaaatatt tgtaatgttt tgctgcgtaa ccttctgctt ctttaaaaag
    10141 ttttgttcct aatccttggt gatgatagtc tttttttatt cccatacaat agaatagatt
    10201 tcggcagtat cttcacttgt ctgtgaaaga gtgataaagc ctaaatattc ttcttttctt
    10261 ttttcacacc ataaaggtag tttacttgat tcctcgatat atttttccgt tgattcagga
    10321 agcccaaacc actcaggcaa gtctagtaat atttctcgga cacactttcc tttttcctct
    10381 ctatctttta caaacgatat cggcatattc tttctagatt ctcccttttt gaattgagta
    10441 tagcatgctt tctttctttt cacgtgctat aataagttaa aatttcaaag ttttacgtaa
    10501 ggagctattt gatgacaaaa aatatttttg attccatcgc taaccattat gattcccctg
    10561 accgtcaagc attagccaag attattcgcg aagaactaac tacttatcta ccgcgcgaca
    10621 gccaccaaaa agtcctgctg gattacggag gcggaactgg gcttgttagt ttaccgcttg
    10681 cggaaagatt caaagaactg atcattgccg atgcttcaga aactatgctg aaaatggcag
    10741 aagaaaagat ccaagcagca gatctgaaaa atgtccgaac gatccacgct gatgcttctg
    10801 tcgaatttcc tgctgtccaa gctaatctca tccttctatc acttgtactt ttacacattc
    10861 ctgatacgga gaatattctt acaaaacttt atgaaatttt agctcctggc ggtcaattga
    10921 tcatcgtaga ctttgacaag aatgaacaaa ttagtcatcc gaaagtccat aatggcttta
    10981 gccaagaaga attgaataat cgattggaaa aaacgggctt tgtctctgct actagtcata
    11041 ccttccatcg tggggaaaag ctatttatga acaaaaacgc ttcgcttttc ttatccatca
    11101 gccaaaaagc ttaaaatgag attgcaaaaa aagtgagcaa ctccaagaaa taagaaggaa
    11161 ttttcggaaa aagaagctgt gacaaaaaac atgtcacagc ttctttttat tttttcatca
    11221 catgatttta atttctgaaa gaggccaata acggaaaaca acttttcctt ggatcatatc
    11281 tttgtcgact aacccaaaca ttcggctgtc ttttgaaatc aagcggttat cgcctagaac
    11341 aagatacttt ccttttggca cagtatattc aaagtcactc gtaaaggtgg atgaactttc
    11401 tgcttgtgct tggaatcctg agctataagc atattctcct tgtaacttat cttctgaaaa
    11461 ttcttttttg aactcatcca gataaggttc atcatacttt ttgccattaa tggtcaactg
    11521 gtcattttcc atcttgactg tatctccagg cataccgatg atccgtttaa caatcatccg
    11581 ttcttcatcc cccggttcct ttgtagtaat aatatccatt cgttcatagt ttgagatctt
    11641 agatgaaatc aatcgttgtc catcacttaa tgtagggtcc atcgaatgcc cgtttacagt
    11701 aactggtgta aacacgtaca ctctagctaa taaaatagcc gctaatacaa ggatcaaaat
    11761 cccccaattt ttcaaaaatt ctttcacagc ttacctccta cttgtatttt cctcgttatt
    11821 tattacgttt tttcacaata cttcgttgta ctttctttat tctacaatgt ttataaatga
    11881 aaggaaagaa taagtgatgt gttcgtctta tctttctcgt tatataaaaa aggaacgtga
    11941 caaaagacgt gtcacatccc ccttattccg aataaacgtt tttatcgcaa ccactgatac
    12001 acggcgtttc atcacattat gaatgtcttc ttctttggct acgactataa accactggtt
    12061 cttcctgcga ctgtgccttt tttgcaatca atttcagacg ataaggctca tgatagcgta
    12121 acccgtcttt gacaaaatca ccgccgacga tgaacaatcc gacaggttcc aatgtgtctt
    12181 ctgttgagtc aagtcgaatg gtccctactt tttccatgtc cgagtagctt cgtccaatat
    12241 ttagaacata ctctttctct cctgttttct tgacatagta ataaggatac aatgtatttc
    12301 tatcgaccag aaatccatcc atgcgaccga gttcatttgt tttgatctca ccatttccaa
    12361 tcaaataccg catattctcg gctaatttca attgataatc ttcgtcataa gaaaccatga
    12421 cctcttgtgt attcacttta tcgatcgaat ttccattatc tatcgattct tctgtctgga
    12481 tgttgaacgc ttcatcaggt aaatacttca caaaaagctg agtgacatcg gctttcgtgt
    12541 ttttatctaa aaagtaataa aatacattta aaacagtaaa ataaacaagc actagaaatg
    12601 caagtccata taagaaaaat tgaagatagt tttggttttg ccaaagtcca taaacaattc
    12661 tcaataaata aagcgtggga atcacactta aaatcgtata tccacggtta agatatttag
    12721 gactgatgtc taaatagcca aggaatttat gcaccccgtt gataaggtct agtaaaaatg
    12781 tcattgagaa tcacctgctt gctggttata cgtttgggta tttcctgaat cttggttttg
    12841 ctgtgctgta cgtgctgcat catcattttg cgtgttatca tctacagtcg tactgcttgg
    12901 tatcgtttcg gagctagcac ttgtatccgg acttgtactt ggattggttg aatccgatgg
    12961 agtgccatct ggtaccgtat tacttgtttc gcttgagctg tcagttgtat atgtctcatc
    13021 atacgacgaa ctagttgttg tatcgtccca tgtacttgtc gtagtggttt cattcaaact
    13081 ttccgttgtt gttggttcag atgtcgagac tgaagtagtt ggtgttgttt cttctacagt
    13141 actagaactt gaacttggtg cacggtagct ggaactattc acaatacctg tagtagtcgc
    13201 gacaatcgtc gagattgtta atacagcgat tggcttcaaa ataggtgcta cttttcgcat
    13261 cttatctctt ccttcattga aatttattct tgtttcctta ctttactacg gacaaattat
    13321 acagtctacc ttatttctac cataaattct ttattttttt cttacgtttt tccgaaagat
    13381 ttctttaatt ttcctcaaaa actacggttg atgtttttaa gtaagctttt gcttcccgat
    13441 tgatttcttt cagatcgcat ccttgctgct tggcagcgaa cacgcaacca caataacatt
    13501 ggcgataaat atcatactct ttgcacattt gcaaagagcg ttcatagcct ttgtttttct
    13561 tgaaatcact tggcaagtat tgtgtcccgt aaatcttttg gatatccatt ccgatctgat
    13621 tgatcaaagc tgcatttttc tttggcgaga tggtcagggc acttccaaag taatcgtatc
    13681 ctagttcttg cgctttttgc gcaacaagat ctaatcgcat attgaagcat gcactgcagc
    13741 gagcaccacc ttctttttcg ttttcaagat ggtttgttaa aaccattttg gtgaattcat
    13801 ttggacggta ttcatcaacg attagcgcaa catgatggtc agttttctca ttaaattggt
    13861 ctacaaatcg tttttgttca tgtaatcttc gcaaatactc acttttagga tgaatattcg
    13921 aatttgaaaa aaataacgtg atctctgcat attgagacat gaattccaat gtgtgcgtac
    13981 tgcaaggagc acagcatgta tgtattaata tcttaggctt tacttcttct ttctcccatt
    14041 gcatgatcat ttttttcaga actgtatcat aatttatttt ttgatttttc attttttcta
    14101 cgatttgttg agcattaatc aatttgttat tcctcctcct tcttttttct aggattttcc
    14161 ttattataag ccatttctat tttaatcgaa agctgtattg ttatctgttt gataatttct
    14221 ttgagtattc ctaattcccc ttgtatactg gtagaaacga ctattgtcag gaggatagta
    14281 ctgtgcatgt tgatttattc tttcgaaggc tgcagcggcg aggacagaag tttgctgcca
    14341 atcatttctc atcgatccaa atcatcgttt tttattatat attgatgacg gtcttatcac
    14401 tcgttctatt ttatatgccg atttttcgcg aaccagatag ccatgtttct tttgtggaca
    14461 tgctgttcat ggcaatcagt acagtgagtg tgactggact atcgactttt gatatcaatt
    14521 ctgtgttcaa tgataacggt attatactgc tggaaatatt atttcaagtt ggtggtttgg
    14581 ggatcatgat gatttctact gcttttgtca ttttttctaa acgacggatc acactgaaac
    14641 agcgacagct gatcatgacg gatatgaatc aaccgcgttt gtctggtatc gtccgactga
    14701 ttcggatcac ttttgctatt ttaatctggt ttcaattact ttttgggaca ttcttttcta
    14761 tttattttta ttacagaggc tattttgatc gttggcgtga tgccgttttt tacggttttt
    14821 atcaggcgat atcggctgtc acgaactctg ggtttgacgt cactggggat tccatcaagc
    14881 cctttgcaca tgactatttt ttcttgatcc tgatcatgtt tttgattttt gtcggaggaa
    14941 ttggttttcc tgtattgatg gagtgccggg aatggctatt atacaaacgt tccaatgcga
    15001 agcttccgtt tcgtttttcc ttgtttacca aattagctgt gttagctttt gttatcttgt
    15061 ttgtttcggg aactgtcttg atttatctat tggaaaagga ccatttattc caagattcca
    15121 atatgagtgt caaatggatc aattcgatgt tttattcgat tacgactcgt aatgctggtc
    15181 tgcagattca cgatttgggt gattttcaaa tcacaacact gatcattttt tctttactga
    15241 tgtttatcgg atgcagtcct agttctgtag gcggcgggat tcgaacaact actgtagcaa
    15301 tcatcggttt gtatctatat tccttcctca aaagtgaaga taacatcaat atttttggac
    15361 ggagaatcga tcaagatgat gtgcgaaaat cagtcgttgt ctttatgctt tctcttggta
    15421 tgtgtttctt ttgtatcgtt tttttatctg ctacagaaga acaaacgctg atctcgatca
    15481 tcatcgaagt aacttctgcg tttggaacaa ctggtttatc tttagggatt accggagact
    15541 tgtctgttgt gggcaaaatc acgattgcta cactgatgtt tattgggcgg atcggaatgc
    15601 tatatacatt gatgttattc gtgccaaaag aaacacgaga tctaggctat gaatatccaa
    15661 gcgagaaaat tattatcggt taaaatggga atataggtaa gagggaataa taaaatcaca
    15721 gcagcttgaa acaatcattt tgtttcaagc tgtttttttg ttaaatcatg cgattttgtc
    15781 cgatttcatg cactttcagc cgtaaccttt tcttaagaat aagaaaagca atttaacctt
    15841 tttttcaaca tatggtatga ttagttgaat ttattcgtca gcaaatcgtt tttggaggtt
    15901 acataatggg aatacgcagt cattttgcga ttgcagttgg aaaaacgtcg caatgggcat
    15961 taaaaacttt ttttaaaggc ggatcaagtt taccaggtaa actagcgctg aaagtcgatc
    16021 cacatatact agatagtttg gcaaaagact accaagtcgt cgttgtcacc ggaacaaacg
    16081 gtaaaacatt gactaccgct ttaacagtca atattttgcg ccagcaattc gatgaagttc
    16141 taaccaaccc tactggtgcg aatatggtac agggaattgt atcgactttc ttgcaggcaa
    16201 aaccaaaaaa aggacagaaa aagtttgctg tcctagaaat agatgaagca agtttgagca
    16261 aagtcacaga atatatgaaa ccagaactgt tcttatttac aaatattttc cgtgatcaaa
    16321 tggatcggta tggagaaatc tatacgacct ataaaatgat tgtagacggt gccgcaaagt
    16381 caccaaatgc aacgattatc agtaacggtg attcaccaat cttcaattct atcgaaacag
    16441 tcaatcctag aaaatattat ggctttgacc atgaagaaga tcgtgaacaa atggctcatt
    16501 acaatacaga cggtgtctta tgtccaaatt gtcatcacat cttgcattat aaaatgatca
    16561 cttatgcaaa cttagggaaa tattactgtc caaattgtga cttcaaacgt cctgaattgg
    16621 attatcgatt aactgatatg gtacggatgg acaatgtgtc agcagatttc gtgatcgatg
    16681 gtcatgaata cggaatcgaa gttggcggta tgtataatgt ctacaatgct ttagcagcta
    16741 ctgcagtggc agagtacttc aatgtcgatc ctgataaaat caaacaaggt ttaggatatg
    16801 acgagaaagt attcggccga caagaagtca tcaatatcaa tgggaaaaaa tgtacattag
    16861 tacttgtcaa aaatcctgta ggtatcaatc aagtaatcga tatgattggt ctagctcctt
    16921 atccattttc tttagtggct ttattaaatg ccaattacgc agacggaatc gatgtcagct
    16981 ggatttggga tggtaatttt gaaaactttg ccgatatgga tatcccagct gttatttctg
    17041 gaggagaccg tcatacagat atgactttac gtctaaaagt tgccgggatt ccagaaaata
    17101 aattgacaga aacaaaagaa ttaccagaag taatcgaaaa aatcaaagaa ttgccaactg
    17161 agcatgtcta tattttagct acttacactg ctgtattgca gcttcgtaaa gaactagctg
    17221 ctcaaggatt tatcaaagga ggaatgaacc gtggctaatt atgaactcaa tatcgcccac
    17281 ctttatggaa atttgatgaa cacatatggt gataacggaa accttttgat gttgaaatat
    17341 atagcagaaa aaatgggggt ttcttgccat acggagatcg ttagtataca tgaacctttt
    17401 gatgctgata aatatgactt ggttttcttt ggcggtggtc aagactttga gcaattaatc
    17461 atttcaaaag acatccagaa aaagaaagat tctttgatca actatatcga aaatgatggc
    17521 gtcatgttag ccatttgtgg tggctaccaa ttgctaggtc actactacat gggagctaat
    17581 ggagaaaaga tccaaggaat cagtgcactg gatcactaca cgttaagtca ggaaaataac
    17641 cggtttatcg gtgatatcgt tatccataac gaagaattca atgaaactta ttatggattt
    17701 gaaaaccaca atggtcgtac tttcttaggt gaaggtgaac gacctttggg gaaagtggta
    17761 caaggtcaag gaaataatgg tgaagaccaa tctgaaggtg tcatttaccg aaatgtcttc
    17821 ggttcttatt tccatggacc tatccttgca agaaatgaga acctggcagc tcgtttgatc
    17881 cgcctagctt tagaacaacg ttacggtaaa gaattggatc tgccctatct tgaaggacaa
    17941 acagcctagt tattcgaaaa aggggaatgt gacaaaaatt tcgtcacatt cccctttttc
    18001 gtatcctatt ttaagcgact tgcttggcat aacggttcac gttttgcttg atattcccga
    18061 ttgttttcgg atcattgatt cctgtgactt cttgaatcaa ttgttccaag ttttcatgga
    18121 tcttcatttc atgcaattga cggctttgtt catcttcagg atcatagtaa ttaaaaataa
    18181 tccccattgt tgccgtcaaa tgtgggcaag tcaagttatg ttcttgtagt tctctcactg
    18241 gacgtgtaaa tctttcttga taacctaact tacgtaatgg tgtccgagcg acacgtgtga
    18301 tcgcatcaga gatatttttg ttttggaagc gatgaatgat tttttcaata tacgcatgat
    18361 gctgctctgc atcaaacccc catttggcaa tcagcaaact tcctgtttcc tgcaaaactg
    18421 attttaattg tgccacaact aacgcatctt gcattgcttc atcaatggtc tggtaaccta
    18481 acaaggcacc tgtataagcg acagtcgcat gaccagtatt gacactaaat agtttgcgtt
    18541 cgatatatgg ttctagatca tctacatata aaaccccttt taattggatt tttttatttt
    18601 ttctttggct atcgtcaatg acccattctt tgaaaggttc cacttggacg aataaaggat
    18661 cttcatggtg ttgtaatggg acgattcgat cgactgctgc atttggaaag ccgatatatt
    18721 tgtcagtaaa tgatgtttcc ggaagatatt tatacacttc tttttctaga aatgctgaac
    18781 cgccaatcat attttcacag gcaatgatat ctaaagatgt tgtgcttcct tccttttctc
    18841 tttcttggat accttttgca atcaactcag caataaaagg taagatattc gggccaatgg
    18901 cggtagtaac taaatctgct tcagcgattt cttggattac tttttctgga tttgtttgat
    18961 tgttgattcc tttgacattt tttacagtga ttttttcttt tgattcatct gctaattcga
    19021 tttcgtattc ttttctttca tttaaagcat caatgatcgt gtgattcaca tctacgaaag
    19081 taatctcaaa tccattagct gccaaaacct ctccaataaa tcctcgaccg atatttcctg
    19141 caccaaaatg tactgctctc atttttctct ctccttaagc aattgctaga ttttccgtta
    19201 cttcttcttt gctgagcgcg tctgctagct ggacaacatt gtccatatca ctgcaataaa
    19261 gtgcgatttg ctgtacaagt tgcaaatgct catctccgac tcctgcaata ccgaacaaaa
    19321 cagtcgcaat cttttcttct tcttcggtac caaaatctac accatctggt acttgaacga
    19381 cacagatgcc ggattttttc acatattttt ttgcttcatc tgttccatga ggaatcgcga
    19441 taaaattccc catataaaca gataacattt tgtctctatc taccatggca tcgatatatt
    19501 cctcatcaac gcaccctgct tctacaagca tctgaccgca atagcggatc gcttcttctt
    19561 tggttttaaa agatcgattg agctcgatca tgtcgaatgg caaactttgc atgctgtttt
    19621 cctcccttaa gctgtctgtt tcaaatattg tgcaatcgat tcgtattttt gcggattgac
    19681 catgctgttg actgataaat gaatggattg aggtgcagcc atctgtactt tttccgctaa
    19741 actttctttg gctacgatca gtgaatcagg ttcatctttt aaatcttgga tattgacttt
    19801 tcttaacggg atagttaaat ggttcttgtc taatatatct ttgagcacgg tcatccccat
    19861 tgttgctgat ccggaacgat catcgtggac aagtagaatc tctttgattt gtttgatgtt
    19921 tgctgaaaga aactcttctg ctggctcatt ctcaatttca gaaactgatg ttttctgctt
    19981 gctgcttaat tcttgaatga tttcatcata tcgaggtgaa ttcaaaaagt tttctactga
    20041 gacaaaacgc gcgctcggtg ctttttgttt tgctctttct tgtagttcga cctgagtaac
    20101 gatcaatgta ttcgagtcat ctgtcagttg gctgattgct tgattggtca ccgtttgagg
    20161 caagtctgct tcctgtactt tttttctcaa gatcgaagcg cccatcgcac ttgatcccat
    20221 tcctgcatca caagcaaaaa tgattttttg tacgccagag aatgtagctc cttgaggaat
    20281 accttgggca cctttggaag cttgcttagc tgcctttgtc tcttgaacag atttttcgaa
    20341 gtcttcattt gtatctttcg catccgcttt caaaattact gcagaaatac caaacgaaac
    20401 agctgcggca acaatcacgc ccaaaataac aggaaggtaa gaattgaacg gtgtcatcgc
    20461 tagaatcgca atgatcgacc ctggagaagc tggcgctctt agtccagcat ccatcaattg
    20521 gaaaatgaaa cttcctgaca ttcctccgcc tataactgat aagaataata acggtttcat
    20581 cataacgtaa ggaaaataga tttcatggat cccgcctaaa aagtgaatga tcatggctcc
    20641 tggtgctgaa gattttgctg ctcctttgcc aaacaaagta aaagcaagta atacgccaag
    20701 tcctggacca ggatttgctt caagcaagaa tagaatagat tttccagcct ctgctacttg
    20761 ttcagttccc aatggtgtca aaatcccatg attgattgca ttattcaaaa ataagatttt
    20821 tgctggttca ataaaaatac ttgtcaatgg aatcaaatgg gcagataaaa tgatctctac
    20881 accttttgcc attgcttgag tcaatgcatc gacaactgga ccaatcgcag aaaaacctaa
    20941 taacgctaat ccaaaaccga taagacctgc tgaaaaatta ttgactagca tttcaaaacc
    21001 ggatttgatc cgtttttgga atagctgatc aaatttcttg atggcatatc caccaagagg
    21061 tcccatgatc atcgcgccta gcatcattgg aatatctgtt ccgacaatca cacccatcgt
    21121 tgcaattgcc ccaacaactg atccgcggtc accagcaatt acttttccgc cagtataacc
    21181 gatcaataaa ggaatcagat aagttagcat tgggcttacc atggtagcga aagcctcatt
    21241 tggtaaataa ccatctggga taaatagagc cgttaataca ccccaggcaa tcagtgctcc
    21301 aatatttggc atgaccatgc ttgataaagt actgcctaat ttttgtaccc tcgcttttag
    21361 cgaaatgttg tttttgggaa tcgtccgttc cattttgtct ctccctctct ccttttgata
    21421 ctctaattgt atccgcttac taaaaagaga aatagtcgta ctttggcacg aaactttgtg
    21481 ccaaaggttt gagtatgtca gcaaaataaa aagatttcgg tatcagaaaa cttcaatttt
    21541 aaataaaact gttattttaa tttttttata atatgcaaac aataactttt gttagcattt
    21601 aatttagatg ttttccattc tattatgtta ttaaatttta tattggaggc ctatctatga
    21661 cattactgat caaaaacatg aacatgaaac gtggtaaaaa acgtgtgatt cgacaattga
    21721 atcttttagt agaatcagga gaagtagctg ctttgattgc cccgaacgga tttggcaaaa
    21781 caactttgct gaatggcatt gcccaactgc tgccaacaac ttatgatcaa ttgtcccttc
    21841 acaacatctc tcctactgct tatacagatt atcgccggtc tttctttttt ctagaatcaa
    21901 cgaatcagtt atccgactat ctatcggctg ttgattattt gactttgatc aaatactatt
    21961 ggcagagcaa agtagaatgc caaactgttt tccaacgatt gaagatgact gagtacctta
    22021 ctttgccgat tggaaaaatg tctctaggaa tgaaacaaca tgtcttgctt gctgcttact
    22081 tgatcagtga ttcatctgtc atgttattcg atgaaccgtt gaacggatta gacccaggaa
    22141 gtattgatat cttgaatagc ctcatcagac aatggcagaa agaggggaag acgattcttc
    22201 tttcttccca cgctatcgcg aatatccaat cgatttgttc taaagcatgt tttctaaaaa
    22261 atggagaaat cgtcacagaa tcctcaaata tggaagagat tttagctatt tatcgtaaat
    22321 tatatgaggt gtaactatgt ctttacttac atttgaacgc aatctcttac taaaaaacaa
    22381 agtaaacttt ttatttcctc tattgttggt cgtgctcttt gcattcccac ttttttttga
    22441 tcataaactt gcttatacag aattcgatga actgaatcat aactatgaag agatgcaacg
    22501 tttgattgaa acgttgaaag aagatgagaa tgagaaagaa tttgtggaaa gtttagagaa
    22561 aagcaacaaa ctgatcgaag caatcttaca tgcaaaaaac acagggaatg tccagcaaat
    22621 ggtcgaagca acttatcatt atgaaaaaga tatactggat cgactgattt ctggtcaaag
    22681 acaaggaatt ccaatcattg aacaacaaaa acgtgtcgag ttgctccgtt atatgaaaga
    22741 acatcagatc caaagatatt ctatttttga cttgccagcg catctctctt tagcaaatta
    22801 ctacgaaaat attttttcag gaatgatttc tagttttttg attttatgta taaccgcctt
    22861 gtttctttct tccatcatct cttatgaaaa aagaaagcag gtaatctctc tagtcaatct
    22921 attaccggat agtatggtaa agaaacatag catccgtttt accatttatt atggtgcagc
    22981 tatgctaagt ctcgtgatgc catttttgat cgtctctata ctagtaataa tcaaaaatgg
    23041 tttgggagac ttccgttatc ctgtaggaac gatcattggt caggagatac gaatcctgcc
    23101 gatgtatgag tatcttttcc agagctttct gttcctgttg ttgtgggtgc tctttttaag
    23161 tacaatcagt tttcttctat ctgctttatt tgaacattct ctagtcaatc ttttaggtac
    23221 acttctttgt cttttcttag cagagtatcg cttgttttct tcgattggat ggatagaatc
    23281 aatcagtcat tacttgccaa cttcttatgt tgattttcaa aatgtgatca taggaggaga
    23341 tattttctcg ccactagctt cagaacaggt tactttcatg aatggtatct tgacacttgg
    23401 tatatggagt atcgttttat tattcatagg tatgggcact atctatataa aaaagagcta
    23461 ttaaaacaca aaaataatag gggcggtgac aaaaaccttg tcaccgtccc ctatttcaaa
    23521 taaacggttt tctagaataa atagttttca aaagcttgca tccgcagtaa taagaacctc
    23581 ccaacaaaaa tagctcctct tatttttatc ttggttgaag ttattacttg atgcaaggca
    23641 gcttttttta ctgtgaacat tggttatata tgtccatgtt caaaaatcat ttggacttct
    23701 tcaatggttt ccgcatcact gagcgccatt acttggtcga tgtctgaaca gaaaaaggct
    23761 aaatcttgga gcaccgctaa ctgatgttct ttttttaaag ccactacaaa taagagtgtc
    23821 gcgatttttg gttcctcttt tgttccaaag tccacaccat caggcacttg gactaaatag
    23881 attccttctt ctactacttc ttcatctttg tcagtcccat gtggcaaggc tacgaaattc
    23941 ccgatataga cactcgcttt ttcttgtctt tcgatcattg attggatata tgccggtttg
    24001 attccccctt gttgggtcat ccactggccg atctcggtca acgcttcttt ccaattttga
    24061 taggattgat cgagcaatat tttttctgct ggtagctgta tcatcttttc accttcattt
    24121 ctttatttct tcaatcagga ttttcgctaa caactgataa acgatttctt tatttcctga
    24181 gcggaagatc tcggtgttca agtcattcat gatgattgct ccactgatct ttcctaaaag
    24241 aatcccgtct ttttcttgga ttggcgaagg cgccagcata actaacatac gggacaaatc
    24301 gactggctgt ctatccatcc cttcaatggt caacggatgt ttcaaattga aaatacaaaa
    24361 gatcggttta gaaatcaatg agctggatgt atggaataac cccatatttg tatgaggaat
    24421 accgataggt gcttgttcat atcgcttaag cagccggcga tgaaccacct cttcatccgt
    24481 tactagtcct tcaggaaaat aaggaaataa gctgcgaatc gtttcggaaa tcgtctcagg
    24541 attatccagt tcttggataa aaaaacgacc gagtaattga ttgatttgat ccatgaaatg
    24601 cattgtttcc tcataagtat cttctatcat ccactcgtta ttgctgttat acgtaatttt
    24661 ttgccttttt ttcttctcaa tatgcgaaaa agcttctttt agctgcttga cttcatcttc
    24721 tagcagtagc ggggagacta aaatatattt tccaccgtat cctggtaaaa tagatgtaga
    24781 aataacaaca tcatattctt cttgtaaatc gatttttttt aaatcagcaa tccgatagaa
    24841 atgaatttgt tggatgaagg gaaaatacct tttcaatcgt agttccaaca tgctagtaga
    24901 agctaatcca cttggcgaaa atcctgcaat actgatattg ctttctttcg gacttcgttc
    24961 caatgaattc gcaaaatgaa ggaccatata agcgatctct tcttcggata attttttttc
    25021 ttgaaaaatc cgctctactg ctttacggat tgcattcgca acttcctcgt attgaaccat
    25081 gattttttct aaaatcggat tggtcagtgt ctcttcttgt aaaacggctc ttgaaaacac
    25141 accagataaa tgagtcaaca gcattttgta caaagtttca tcttcataaa aatggacatt
    25201 cgtctcttca cttacttctt gaatcagttg ttttactaaa taagcaagct gtgtatcgaa
    25261 cgtctcttca aagaaattct gatcaaatga gttcgtgaag tcatttaata atcctgcaaa
    25321 aaagacgatc tcatttactg gataaagttg ttttgtctca gaagcgatcc gtgaaaacaa
    25381 tcgtttagct gtctgtagta attctttgtt tgccagacta ttatatgtct catttgtcag
    25441 taaatgattt ttcgtcacac gctccatcgt caatgtcagc atcaaaacca aatgttcaag
    25501 ctttctatca cttagattgg tctttttttg gatcaattct tccccgatta ctttgcgtgc
    25561 aagagataga tgttgcggat caacgaatcg aaaaaaataa ttcagtgatg catcgaaaga
    25621 aatactatga aaaagttcat attcattgat ttctaattct aatacattcg ctgtcagaag
    25681 ccggcggtat ttttccggtc cttcgatctc gtatccctgg ttccgaatga tttccaaagg
    25741 cagttggcga atactttctt ccaactgttt gatatctgca taaaaagtgg tattactgat
    25801 gcgatatgat tccatgaaat gagcaagact caacggctct gtcgtcagta acaactcggc
    25861 taaaatcgcg tgttgtcgtt tagcagtgga taagtcatta cttttcccat ctgtattttc
    25921 ttttaattgc tctaatactt cttctgatgc ctcaatgaca aatgagcctc tagctgcttt
    25981 tttcaaagaa gcatttaccg atttcaatga gacttcaaga ttatctaatt cgcggtaaat
    26041 agtccgttta ctgaccttca ataaattcat cattttttgg atagatacag gtgacgggct
    26101 actgagaaat tctgctaata attgtttttc tctgggcgat aaatacatct tatatttctc
    26161 ccttcacgag tttttcgtat tcattcgtag ctacccaatg ttcaactaga actagccgag
    26221 ggatcttttc ttctagctgt tcagttacag ctagttctct ttgtgcgata taaatcgttt
    26281 cttcttggat tttgattttt tctattgatt cttttgagat aacaaactgt tttttcgctt
    26341 tttttgctaa ttgacttaaa atcgtcatgc ccatagtttg tgagccgcgt ctgttgtttt
    26401 tatacaaaaa tacaatatta gcaggcgacg ttttttgttc tgtgatatct gctatctctt
    26461 ttttttcttc ccgctttgga aattggatcg cttcttttat tcgtgacagc aagatatctg
    26521 tttccaaaaa gtgatccagt acatagattt gactttgagg tactttttct cttgcaagac
    26581 ccgctagttc atgttgcacg ataaccagct gatttacttg gttattcaat tcatagatag
    26641 agctgtatcc gacagaaaga tcgatttttt ctgctgctaa tttctttttc agcagggcgg
    26701 ctcccatagc acttgatccc atacctgagt cgcaagcaaa aatgacggaa tgaatgatag
    26761 atggttccaa gatttctccc ctttctttga ccgttttgtt ctctttttct ttatttggta
    26821 atttcaatag tatcgctgca caaatgaagg tcacaaatgt actgattgct atccctatac
    26881 ttaccccagc tagttgtgga taaggcgcat tggcaaaaat cacgagtagt gacccaggcg
    26941 atacaggtgc agctaatccc gtatctaacc attgaaatat caacgtgccg cttgccccac
    27001 cagccattac agctaaaaat agatacggtc gcagcaagac aaatggaaaa tagatttcgt
    27061 gaatcccgcc aatcgcatgg atcatcaatg ctccactcgc atttactttt tcctgttttt
    27121 ctgcaaaaca aaggatagaa agtaagatcc ctaaaccagg tccaggattt gcctccatca
    27181 aaaataagga agagtacccg actgtttgtg agaattcgat tcctaaaggt gtcaatacac
    27241 catgattgat tgcattattg aaaaatagta cttttagagg ttctaagatc agactgacaa
    27301 gtggtaacag attcattgtg actagccaag acacaacgga ggcgagcaac aagtgaacac
    27361 tatttaaaac tggttcaata aaaacgattc caattataca aataacgcta ccagtcagtc
    27421 ctactagtag gttgctgaca agcatttcat atccagcctt gaatttcaca tggaatctag
    27481 aaacaaattg ttcatatacg atgccggcaa gcggcccgat aatcatcgct cctacgattt
    27541 gtggagcatt ggaacgagcg atcaacccta aagaagcaat tgcaccggcg actgcccctt
    27601 tttgtccccc taccgatttc ccgcctgaat aactgatcaa gatgggtaaa agcagctgga
    27661 tcatccaatg gcatacctct tctaatgtgg tgtgcaaatc gccagacaca ccttttgttg
    27721 ctaatgacaa gattccccaa gcgataaaca gacttagatt tggcatgatc atttcattta
    27781 agtgatgtcc agcttttctc agttgtttca tagacacgcg actgctgttg tttgctttcc
    27841 atttgtattc catctttttc accagacttc tatttattga tggacggatt ttatgtagaa
    27901 aaagcagcaa acgaaaaacc gtctgctgct ttcagctatc tgggaactat ttgcctggtt
    27961 cagacgcaat gattgctagt tcaccttgga ccgcccactc agaaacgcca tttggaataa
    28021 tacagcttgt tcctttttct aatggatatg tttttccgtc aacgacaagg tctcctgctc
    28081 cttcgattac cgtcatcaat gtataagggg cttgtttttt gaaagatgtg attcctttaa
    28141 tatcccactc atatacattg aaaaactcag tttctaaata agtgacgatc gatgaattcc
    28201 cttttcggac ttctttgatt tgtaattctg gtgttttcgc agggacagtt gttacatcga
    28261 tcgattgttg gatatgcagc tctcttgttt gtccttgcgc atcttttctg tcgtagtcat
    28321 acacgcggta agtcgtgtca gaactttgct gtgtttctaa aatcatgatt cctttaccaa
    28381 tagcatggat cgttccgctt ggtacataaa agaaatcacc ttttttaact ggtactttct
    28441 tcaaaaggtc atcccagcgc ccatctttga tcatctcagc tagttcttct cttgtctttg
    28501 catgatgacc atagatgatt tctgcaccag gttcagcatc gatgatatac cagcattcag
    28561 tttttcctaa ttcaccttca tgtttcatcc cataggcatc atctggatgt acttgtacag
    28621 atagatcatc ttctgcgtct aagatcttga tcaataatgg aaacactggt tctgaaggat
    28681 ggccaaacaa ttccttgtgt tctgaccata gttcatctaa tcttttccct ttgaattcgc
    28741 cattttctac tacactgaca ccatgaggat gcgcactgat cgcccaatct tctccgattt
    28801 tatcgttagg gatatcaaaa ccaaagactg aacgtaaacg acttcctccc caaatttttt
    28861 cttgaaaaac tggttttaaa aacataggtt cctgcataat tcaaactcct ctcatttttc
    28921 atagctatta tagcattatt tcggtaatac cgctttctct ttcaaaaagt tttcaacaat
    28981 ttcatctcga catatgagat actccgcacg atgatcgaca ggatgtacgc gatttgacaa
    29041 aaatatcaac gcttcttgtg tttttatatc aatcaagaaa aacgtacctg tataaccagt
    29101 atgaaaaaga atcgcatgac cgtcttctac atcctttttt aagtcccatc ctaaagaacg
    29161 atagccattt tttttcggtg tttgatcttg cagtaaagaa tcaatcgtct cttcttctaa
    29221 aaaacgacca ttacggattt ttcccttgtt caaatacatt tggacaaaag taatgctgtc
    29281 tttcaaatca ataaagagac ccgcgttacc tgcatgttca gccaaaactc ttgctttcgg
    29341 atcatgagtt tgtccacgca aaacctcacc cgaaggcagt tgttctgtgg gaacgatttt
    29401 tttggaagga aaaggaagaa ataagctgtt catcattcct aatggttcca atacttgttc
    29461 ttgaaagatt tccactgcag tttcttggta aatttcctct agcatcaacc caagaagaat
    29521 cgtaccggca tctgtatatt ttacttcttt tcctagttgc tcacctggtt ctagcttcaa
    29581 ataagcggcg atcaattctt cctgagatag cttatctctg tttggtatcc atgttttgat
    29641 atcagaagta tgtgtcagta aatgacgcaa agtgattgtc tgattttcaa attcaggaag
    29701 atatttatga agcggttgat cgagttctat ctttccatct tctaatagac gtaaaactaa
    29761 tgaagtggtt gcaacgactt tcgtcagtga ggctgcatca aacaacagat cttctgtcat
    29821 cagctgcttt tcaggaacga cttgggcaaa gccggctgta aatgtttcta cttgattttc
    29881 ttttatgaag gcgactgcta cacctggata aaatccttct tccatctttt tcaaaataag
    29941 ctgtttcgtg tgtggataca tctttttctc ctgcttttct atcagattct tacagtccaa
    30001 gcttaaagct taatctatga ggaatcaacc ttttttacac aaaaaaactg cgcgaaaaac
    30061 cgcgcagttt cccccggtat actattttct ctttctatct ctagaataag cgatagtatg
    30121 accgttcata aaatgacgag acatgcgaca atcgttaaga aagaaaaagt taagcgtcgc
    30181 atcgaatgtc agttttattc ttttcttttg gctcactgac gaaccaccat tcgattccta
    30241 tagcatcata gattgtgagt actgtgtgct ttttgtctaa gaagaaatca actgatagtt
    30301 gctgcaagtg agcaatcagt ccttccatgc tttcagtatc gatcatgaat ttcagaaaat
    30361 caagtccaat cacttcatct gtaggcaaat caattgcgcc tccgctagct tctgtcaaac
    30421 caacatggaa atctccttca ttcaatacat ggatgccctc ttcttcatta tgtaccttca
    30481 aaccaagtac atgattaaga aattggcgtt cttttgttaa gttagtaaca tttaaatgga
    30541 ttttttcaaa ataagaacct gttgatagtt tactgaattt ttcttcagat tttcttaata
    30601 atgcttcttg atccattgtt tctggaattt ctctttggtc gttcttattc tcttcatggt
    30661 aaatttccaa ttcattctct tctgggtcta cgagcaaaat ccccatacgt ccatgatctt
    30721 ctaaagcttc tttgattgga taattcattt tacggatacg acaaaggata tctgccattt
    30781 cttctgtgct agggataatc aatgaaaaac ggttcagctt tttgatcacc cccatgtgat
    30841 cattcgctct aggactttct tccagcagca acatttcact tccagcttct tctgttccta
    30901 aaatggctaa ttcgttttct tctcttttca aatcaaagcc aacaacgttc ttgtaaaaat
    30961 caatcatttt atctcggtct ttcacccgaa tggctacact ttttaaatag gtggcagccc
    31021 ccaattgaaa atctttcata tttcttgctc ccctctacgt caagctttat tttaacgatg
    31081 gattcttctt ctttcaagca ataactttga ttttgttaaa aaaaatgtga gaaaattcgc
    31141 attttgcttg ttttttgtaa attaatgagt aaaaactctt ataaaaagag gttgtgaaaa
    31201 agctgttctg catcaagtaa taagaacagc caaacaaaaa tagctcctcc tatttttcgt
    31261 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    31321 aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tctacatttt
    31381 tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttac caagaggttg
    31441 tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcaggtt
    31501 tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt aaggaagagg
    31561 ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca
    31621 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga
    31681 aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta
    31741 ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct
    31801 ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata
    31861 atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt
    31921 tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat
    31981 gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt
    32041 agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct
    32101 tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt
    32161 tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc
    32221 caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg
    32281 gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg
    32341 ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt
    32401 ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca
    32461 ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg
    32521 gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg
    32581 atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc
    32641 aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt
    32701 acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc
    32761 gaaataaatt gagctttcca accagcggca tacagattct gattttcttt ttcaaatact
    32821 tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct
    32881 tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat
    32941 ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac
    33001 gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta
    33061 gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt
    33121 gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc
    33181 attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata
    33241 ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca
    33301 atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc
    33361 atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc
    33421 tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc
    33481 gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta
    33541 ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct
    33601 ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg
    33661 aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc
    33721 caaccagttt tccttcatct agcacaagga tcatatctgc atccattact gtacttactc
    33781 gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat
    33841 tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg
    33901 ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc
    33961 ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt
    34021 gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta ccatactgca
    34081 tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt
    34141 ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag
    34201 attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc
    34261 cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa
    34321 gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc
    34381 cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca
    34441 cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa
    34501 gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga
    34561 cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac
    34621 cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg
    34681 ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat
    34741 cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt
    34801 cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca
    34861 aaataatcat tactgggatg acaactaaga agatttttgt caactgatga tctttttgat
    34921 aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca
    34981 tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg
    35041 ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac
    35101 gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag
    35161 ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga
    35221 catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat
    35281 ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc
    35341 gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc
    35401 aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg
    35461 aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca
    35521 gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca
    35581 actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg
    35641 tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa
    35701 aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc
    35761 ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac
    35821 gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga
    35881 aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg
    35941 gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca
    36001 aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg acacattgtc
    36061 atatgacgct atgtcattat aacacctcct acctgttttg caagacattt gcatagtttt
    36121 tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat
    36181 gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca
    36241 attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca
    36301 agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct
    36361 atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat
    36421 acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt
    36481 tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga
    36541 ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt
    36601 agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt tcgagtaaac
    36661 tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt aagcatatgg
    36721 catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc acagttaagg
    36781 cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa gtgactgttt
    36841 tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca ggttgggaag
    36901 cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa aaaattgctc
    36961 gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc gcaaacgcct
    37021 tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc aatgtagctg
    37081 gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac
    37141 tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct caatatgtaa
    37201 aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc gaaggaatcg
    37261 attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa gctgctgctt
    37321 taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag gaaaaaatct
    37381 tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt attctttctg
    37441 ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt caaaaacatg
    37501 agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac cgatgattcc
    37561 gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga ttgctaactg
    37621 attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta ctgcaaaaac
    37681 gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga tagccggaat
    37741 catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa taatccccaa
    37801 cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga ttgccacagc
    37861 aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca tttcactcag
    37921 cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa
    37981 ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca gagccactaa
    38041 aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct tagatatatc
    38101 ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca actgttcgcg
    38161 aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa tcgtatctac
    38221 atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag ggatgatcac
    38281 tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga tactgtaaag
    38341 ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt aatacaagat
    38401 tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg gtgcaaacaa
    38461 atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa caagtaaggc
    38521 tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc tgttcttctt
    38581 attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc caaaccaata
    38641 gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc ttctggacaa
    38701 gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc catcggaccg
    38761 acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa
    38821 aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc agctgttgct
    38881 gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt cccaatttct
    38941 gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa cagtataaca
    39001 cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg aaaactattt
    39061 cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg aacctatacg
    39121 cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga agaattagcc
    39181 aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag taaagctggc
    39241 aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta cgataaggat
    39301 tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt agcggttgat
    39361 gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa tgtctaccat
    39421 atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga agcgacaggt
    39481 cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc tgaccaacga
    39541 ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc
    39601 aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg ccatcttgtc
    39661 tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac agtccgcgtg
    39721 ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc gaccttgcca
    39781 gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga tggcaagttc
    39841 ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt tgaagatggt
    39901 tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg cgatttcgca
    39961 ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa tctgacatta
    40021 tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt ctatgtacca
    40081 gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat gaatcagcaa
    40141 gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg gaaaatcgaa
    40201 gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca gaactacttt
    40261 gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg aaaagaacat
    40321 caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa accgccgaga
    40381 tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca aggttgttgt
    40441 tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa aggtaatatt
    40501 acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt attttttcaa
    40561 caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt
    40621 tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga tactttggat
    40681 catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt tatgaatgcc
    40741 cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc gatatttttt
    40801 tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca gttcacttgt
    40861 ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc cttctccgat
    40921 tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa tattcggtaa
    40981 catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg tttttatcct
    41041 caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat tcttattcgt
    41101 gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca tattggatca
    41161 attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct ttataccact
    41221 gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct aatgataaaa
    41281 gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc gctgcaaaat
    41341 aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct ttactgtcga
    41401 acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg
    41461 aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta gaaagattct
    41521 tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct tgcagataat
    41581 tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct agattatcat
    41641 actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt
    41701 tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa atatatggat
    41761 tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg tcaaatccta
    41821 atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg atcgctgtcg
    41881 tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc atttttggta
    41941 caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa aaaccagtca
    42001 atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc atattgccac
    42061 tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat acttctacgc
    42121 cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg ttcggcgact
    42181 cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca gtgatttctt
    42241 tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc agattgtaca
    42301 aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa ggtatccata
    42361 gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa ttactgatat
    42421 accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg aatcgccttc
    42481 cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt cctgcggcag
    42541 ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg atttcagtga
    42601 tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc atataatttg
    42661 caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg acagatcctg
    42721 ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag aagaattttt
    42781 ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca ttttgacacc
    42841 actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt gctgctgttc
    42901 cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc
    42961 tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta cgaaattcga
    43021 aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt tatggccttt
    43081 ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc tgttgtttca
    43141 cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag acatgctcat
    43201 tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc agagaaaata
    43261 tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga
    43321 aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac aaaagataat
    43381 cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc
    43441 tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt
    43501 caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat
    43561 ttcacttctt cctacattga taagtacagc atcttttttc attttttcta tattcgcctg
    43621 atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt
    43681 agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga
    43741 ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc
    43801 tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct
    43861 tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc
    43921 ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt
    43981 agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt
    44041 gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg taagccattc
    44101 atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt
    44161 gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac
    44221 gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac
    44281 tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga
    44341 taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga
    44401 gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt
    44461 caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt
    44521 tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt
    44581 ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg
    44641 aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac
    44701 aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt
    44761 ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa
    44821 tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg
    44881 tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg
    44941 gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat
    45001 gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta
    45061 tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc
    45121 ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat
    45181 ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata
    45241 gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac
    45301 gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg
    45361 aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa
    45421 acgagccact tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc
    45481 aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt
    45541 taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat
    45601 gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag
    45661 caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg
    45721 catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt
    45781 gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt
    45841 ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc
    45901 cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat
    45961 aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa
    46021 ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt
    46081 aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc
    46141 atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg
    46201 atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc
    46261 gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc
    46321 tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca
    46381 tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg
    46441 agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac
    46501 aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa
    46561 atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg
    46621 tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta
    46681 tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt
    46741 aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc
    46801 cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat
    46861 ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca
    46921 tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt
    46981 taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat
    47041 ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta
    47101 aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa
    47161 tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta
    47221 gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat
    47281 tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat
    47341 gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat
    47401 tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc
    47461 ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg
    47521 gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt
    47581 cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt
    47641 caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt
    47701 aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt
    47761 tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt
    47821 tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg
    47881 gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc
    47941 gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca
    48001 tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata
    48061 cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat
    48121 aggtccagat accattttct agtttgtttt cccactcata actaccagcg gcaataggag
    48181 taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag
    48241 atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt
    48301 aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt
    48361 attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa taaaaaacac
    48421 ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct
    48481 tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt
    48541 ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga
    48601 ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag
    48661 gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc
    48721 tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg
    48781 gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga
    48841 ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa
    48901 tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg
    48961 gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa acgatggctt
    49021 ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag
    49081 ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag
    49141 tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga
    49201 atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc
    49261 gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg
    49321 ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg
    49381 cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg
    49441 ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac
    49501 tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt
    49561 tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga
    49621 catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag
    49681 cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg
    49741 cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt
    49801 tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa
    49861 gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg
    49921 acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac
    49981 aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg
    50041 agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag
    50101 ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct
    50161 cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat
    50221 ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca
    50281 acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag
    50341 aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact
    50401 tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt
    50461 cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag
    50521 taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt
    50581 tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc
    50641 ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat
    50701 aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc
    50761 tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga
    50821 tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat
    50881 cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc
    50941 ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt
    51001 ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc
    51061 ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat
    51121 gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt
    51181 cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca
    51241 tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt
    51301 caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct
    51361 ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct
    51421 aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt atcaaagcac
    51481 ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa
    51541 gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc
    51601 tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta
    51661 atgaatattt tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt
    51721 ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga
    51781 ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc
    51841 cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc
    51901 cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga
    51961 ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt
    52021 gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat
    52081 tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat
    52141 tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta
    52201 aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata
    52261 ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta
    52321 cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat
    52381 agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc
    52441 tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta
    52501 tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta
    52561 cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa
    52621 tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc atcacataat
    52681 aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta
    52741 ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta
    52801 atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga
    52861 cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta
    52921 gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt
    52981 cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct
    53041 catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag
    53101 ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg
    53161 atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact
    53221 tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca
    53281 cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt
    53341 tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt
    53401 ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt
    53461 tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta
    53521 tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca
    53581 cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt
    53641 cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg
    53701 gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc
    53761 gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca
    53821 acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc
    53881 caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata
    53941 acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt
    54001 caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa
    54061 gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt
    54121 cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt
    54181 tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct
    54241 cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa
    54301 caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca
    54361 actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca ttccagacat
    54421 agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat
    54481 tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa
    54541 agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag
    54601 caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct
    54661 caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc
    54721 ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc
    54781 agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac
    54841 ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa
    54901 atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga
    54961 atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta
    55021 atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc
    55081 aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag
    55141 ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat
    55201 ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc
    55261 catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg
    55321 agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca
    55381 attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa
    55441 attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca
    55501 gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa
    55561 actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc
    55621 cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga
    55681 tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga
    55741 cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa
    55801 tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat
    55861 tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg
    55921 gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac
    55981 taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca
    56041 aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa
    56101 ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg
    56161 tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt
    56221 aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg
    56281 caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag
    56341 ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct
    56401 caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg
    56461 cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt
    56521 cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag
    56581 gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa
    56641 actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc
    56701 catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat
    56761 aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta
    56821 gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct
    56881 tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa
    56941 attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat
    57001 ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag
    57061 ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta
    57121 atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg
    57181 ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag
    57241 atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta
    57301 catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa
    57361 tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga
    57421 tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag
    57481 atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg
    57541 aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta
    57601 accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca
    57661 ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt
    57721 gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca
    57781 ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga
    57841 ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg
    57901 gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga
    57961 atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag
    58021 atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacatttacc
    58081 aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga
    58141 cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc
    58201 tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca
    58261 aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct
    58321 gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca
    58381 tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga
    58441 tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat
    58501 aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac
    58561 cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct
    58621 aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc
    58681 caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca
    58741 cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca ccagttataa
    58801 aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta
    58861 ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca
    58921 ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga
    58981 aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt
    59041 cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca
    59101 ctactaatca acattgtggc ttccataact gacctccttt aatttataca tgaataatac
    59161 acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt
    59221 taattaacca aaatattaat aatgattaaa acaaataatt taatataact tttattaaca
    59281 aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa
    59341 cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca
    59401 gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag
    59461 tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa
    59521 agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca gttttccaga
    59581 aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa
    59641 ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga
    59701 tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag
    59761 cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt
    59821 ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga
    59881 tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc
    59941 tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt
    60001 gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa
    60061 catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa
    60121 agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga
    60181 tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac
    60241 aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat
    60301 catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga
    60361 ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat
    60421 atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag
    60481 tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt
    60541 gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga
    60601 agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc
    60661 cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat
    60721 cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga
    60781 tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt
    60841 catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg
    60901 tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt
    60961 ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt
    61021 tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta
    61081 attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag
    61141 ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt
    61201 agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga
    61261 atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct
    61321 ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt
    61381 attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc
    61441 atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca
    61501 cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc
    61561 catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg
    61621 ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg
    61681 catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc
    61741 cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc
    61801 actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat
    61861 cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt
    61921 atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga
    61981 atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt
    62041 tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg
    62101 tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc
    62161 tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa
    62221 ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa aaactagttc
    62281 tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa
    62341 accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag
    62401 attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa
    62461 aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca
    62521 gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg
    62581 gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata
    62641 acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac
    62701 ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt
    62761 aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc
    62821 gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt
    62881 aagataccga tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa
    62941 gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat
    63001 aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt
    63061 gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc
    63121 tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt
    63181 gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga
    63241 atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat
    63301 tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat
    63361 ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt
    63421 gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa
    63481 cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg
    63541 ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt
    63601 cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc
    63661 attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta
    63721 atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt
    63781 aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg
    63841 tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta
    63901 aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt
    63961 ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc
    64021 ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg
    64081 gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac
    64141 gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg
    64201 aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt
    64261 acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact
    64321 gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc
    64381 ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg gcctgaatat
    64441 cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca
    64501 tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa
    64561 aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa
    64621 acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac
    64681 aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca
    64741 ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat
    64801 ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca
    64861 ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat
    64921 ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct
    64981 ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac
    65041 atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc
    65101 ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc
    65161 gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc
    65221 catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc
    65281 catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct ttatgatagc
    65341 ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga
    65401 tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt
    65461 ttccataact taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg
    65521 caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa
    65581 tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt
    65641 cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa
    65701 aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc
    65761 ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc
    65821 ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt
    65881 gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac
    65941 tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt
    66001 tttattcctc tttcattatt accaaagtct cttattttaa attgaataca ttataatgat
    66061 actgtaaata tatctttatt attagtgaat
//
LOCUS       DAYYHB010000003        63545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000003 DAYYHB010000000
VERSION     DAYYHB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.003"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..425
                     /locus_tag="ACHA6B_000146"
     CDS             66..425
                     /locus_tag="ACHA6B_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8947756.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            548..2179
                     /locus_tag="ACHA6B_000147"
     CDS             548..2179
                     /locus_tag="ACHA6B_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8947757.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2241..2882)
                     /locus_tag="ACHA6B_000148"
     CDS             complement(2241..2882)
                     /locus_tag="ACHA6B_000148"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8947758.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    3068..3144
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3164..3472
                     /gene="rplU"
                     /locus_tag="ACHA6B_000149"
     CDS             3164..3472
                     /gene="rplU"
                     /locus_tag="ACHA6B_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8947759.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3499..3843
                     /locus_tag="ACHA6B_000150"
     CDS             3499..3843
                     /locus_tag="ACHA6B_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8947760.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3856..4149
                     /gene="rpmA"
                     /locus_tag="ACHA6B_000151"
     CDS             3856..4149
                     /gene="rpmA"
                     /locus_tag="ACHA6B_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8947761.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4583..7390
                     /locus_tag="ACHA6B_000152"
     CDS             4583..7390
                     /locus_tag="ACHA6B_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8947762.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7581..8072
                     /locus_tag="ACHA6B_000153"
     CDS             7581..8072
                     /locus_tag="ACHA6B_000153"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8947763.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8175..9164
                     /locus_tag="ACHA6B_000154"
     CDS             8175..9164
                     /locus_tag="ACHA6B_000154"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8947764.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9197..10000
                     /locus_tag="ACHA6B_000155"
     CDS             9197..10000
                     /locus_tag="ACHA6B_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8947765.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            10021..10935
                     /locus_tag="ACHA6B_000156"
     CDS             10021..10935
                     /locus_tag="ACHA6B_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8947766.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            11018..11392
                     /locus_tag="ACHA6B_000157"
     CDS             11018..11392
                     /locus_tag="ACHA6B_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8947767.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11531..11776
                     /locus_tag="ACHA6B_000158"
     CDS             11531..11776
                     /locus_tag="ACHA6B_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8947768.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11948..13012
                     /locus_tag="ACHA6B_000159"
     CDS             11948..13012
                     /locus_tag="ACHA6B_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8947769.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13258..13683
                     /locus_tag="ACHA6B_000160"
     CDS             13258..13683
                     /locus_tag="ACHA6B_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8947770.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13676..14125
                     /gene="nusB"
                     /locus_tag="ACHA6B_000161"
     CDS             13676..14125
                     /gene="nusB"
                     /locus_tag="ACHA6B_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8947771.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14222..15076
                     /locus_tag="ACHA6B_000162"
     CDS             14222..15076
                     /locus_tag="ACHA6B_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8947772.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            15077..16414
                     /gene="xseA"
                     /locus_tag="ACHA6B_000163"
     CDS             15077..16414
                     /gene="xseA"
                     /locus_tag="ACHA6B_000163"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8947773.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16419..16643
                     /locus_tag="ACHA6B_000164"
     CDS             16419..16643
                     /locus_tag="ACHA6B_000164"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8947774.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16645..17535
                     /locus_tag="ACHA6B_000165"
     CDS             16645..17535
                     /locus_tag="ACHA6B_000165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8947775.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17544..18362
                     /locus_tag="ACHA6B_000166"
     CDS             17544..18362
                     /locus_tag="ACHA6B_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8947776.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18469..18918
                     /locus_tag="ACHA6B_000167"
     CDS             18469..18918
                     /locus_tag="ACHA6B_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8947777.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18936..20618
                     /gene="recN"
                     /locus_tag="ACHA6B_000168"
     CDS             18936..20618
                     /gene="recN"
                     /locus_tag="ACHA6B_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8947778.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20673..20789)
                     /locus_tag="ACHA6B_000169"
     CDS             complement(20673..20789)
                     /locus_tag="ACHA6B_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8947779.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20862..21818)
                     /locus_tag="ACHA6B_000170"
     CDS             complement(20862..21818)
                     /locus_tag="ACHA6B_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8947780.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21989..22357
                     /locus_tag="ACHA6B_000171"
     CDS             21989..22357
                     /locus_tag="ACHA6B_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8947781.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22527..22958
                     /gene="mraZ"
                     /locus_tag="ACHA6B_000172"
     CDS             22527..22958
                     /gene="mraZ"
                     /locus_tag="ACHA6B_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8947782.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22979..23938
                     /gene="rsmH"
                     /locus_tag="ACHA6B_000173"
     CDS             22979..23938
                     /gene="rsmH"
                     /locus_tag="ACHA6B_000173"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8947783.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLRQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23943..24344
                     /gene="ftsL"
                     /locus_tag="ACHA6B_000174"
     CDS             23943..24344
                     /gene="ftsL"
                     /locus_tag="ACHA6B_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8947784.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24345..26537
                     /locus_tag="ACHA6B_000175"
     CDS             24345..26537
                     /locus_tag="ACHA6B_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8947785.1"
                     /translation="MSLKNKIWRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDRDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26563..27525
                     /gene="mraY"
                     /locus_tag="ACHA6B_000176"
     CDS             26563..27525
                     /gene="mraY"
                     /locus_tag="ACHA6B_000176"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8947786.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27539..28900
                     /gene="murD"
                     /locus_tag="ACHA6B_000177"
     CDS             27539..28900
                     /gene="murD"
                     /locus_tag="ACHA6B_000177"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8947787.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28926..30014
                     /gene="murG"
                     /locus_tag="ACHA6B_000178"
     CDS             28926..30014
                     /gene="murG"
                     /locus_tag="ACHA6B_000178"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8947788.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            30029..31249
                     /locus_tag="ACHA6B_000179"
     CDS             30029..31249
                     /locus_tag="ACHA6B_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8947789.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31405..32730
                     /gene="ftsA"
                     /locus_tag="ACHA6B_000180"
     CDS             31405..32730
                     /gene="ftsA"
                     /locus_tag="ACHA6B_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8947790.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32753..33994
                     /gene="ftsZ"
                     /locus_tag="ACHA6B_000181"
     CDS             32753..33994
                     /gene="ftsZ"
                     /locus_tag="ACHA6B_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8947791.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            34011..34688
                     /locus_tag="ACHA6B_000182"
     CDS             34011..34688
                     /locus_tag="ACHA6B_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8947792.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34702..35301
                     /locus_tag="ACHA6B_000183"
     CDS             34702..35301
                     /locus_tag="ACHA6B_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8947793.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35382..35603
                     /locus_tag="ACHA6B_000184"
     CDS             35382..35603
                     /locus_tag="ACHA6B_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8947794.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35652..36434
                     /locus_tag="ACHA6B_000185"
     CDS             35652..36434
                     /locus_tag="ACHA6B_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8947795.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36455..37162
                     /locus_tag="ACHA6B_000186"
     CDS             36455..37162
                     /locus_tag="ACHA6B_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8947796.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37424..40222
                     /gene="ileS"
                     /locus_tag="ACHA6B_000187"
     CDS             37424..40222
                     /gene="ileS"
                     /locus_tag="ACHA6B_000187"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8947797.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40271..41797)
                     /gene="zwf"
                     /locus_tag="ACHA6B_000188"
     CDS             complement(40271..41797)
                     /gene="zwf"
                     /locus_tag="ACHA6B_000188"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8947798.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41812..42459)
                     /locus_tag="ACHA6B_000189"
     CDS             complement(41812..42459)
                     /locus_tag="ACHA6B_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8947799.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42643..42972)
                     /locus_tag="ACHA6B_000190"
     CDS             complement(42643..42972)
                     /locus_tag="ACHA6B_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8947800.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43149..43877
                     /gene="sfsA"
                     /locus_tag="ACHA6B_000191"
     CDS             43149..43877
                     /gene="sfsA"
                     /locus_tag="ACHA6B_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8947801.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43893..44906
                     /locus_tag="ACHA6B_000192"
     CDS             43893..44906
                     /locus_tag="ACHA6B_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8947802.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44906..46183
                     /locus_tag="ACHA6B_000193"
     CDS             44906..46183
                     /locus_tag="ACHA6B_000193"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8947803.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46246..48948
                     /locus_tag="ACHA6B_000194"
     CDS             46246..48948
                     /locus_tag="ACHA6B_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8947804.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49100..49417
                     /locus_tag="ACHA6B_000195"
     CDS             49100..49417
                     /locus_tag="ACHA6B_000195"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8947805.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49447..49767
                     /locus_tag="ACHA6B_000196"
     CDS             49447..49767
                     /locus_tag="ACHA6B_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8947806.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49875..51335
                     /locus_tag="ACHA6B_000197"
     CDS             49875..51335
                     /locus_tag="ACHA6B_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8947807.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51403..51624
                     /locus_tag="ACHA6B_000198"
     CDS             51403..51624
                     /locus_tag="ACHA6B_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947808.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51655..51837
                     /locus_tag="ACHA6B_000199"
     CDS             51655..51837
                     /locus_tag="ACHA6B_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8947809.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51837..52250
                     /locus_tag="ACHA6B_000200"
     CDS             51837..52250
                     /locus_tag="ACHA6B_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8947810.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52373..53554
                     /gene="eis"
                     /locus_tag="ACHA6B_000201"
     CDS             52373..53554
                     /gene="eis"
                     /locus_tag="ACHA6B_000201"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
                     /protein_id="HFM8947811.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGENHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53625..53789)
                     /locus_tag="ACHA6B_000202"
     CDS             complement(53625..53789)
                     /locus_tag="ACHA6B_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947812.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53943..54016
                     /locus_tag="ACHA6B_000203"
     tRNA            53943..54016
                     /locus_tag="ACHA6B_000203"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53977..53979,aa:Arg,seq:cct)
     gene            complement(54085..55224)
                     /locus_tag="ACHA6B_000204"
     CDS             complement(54085..55224)
                     /locus_tag="ACHA6B_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8947813.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(55523..56158)
                     /locus_tag="ACHA6B_000205"
     CDS             complement(55523..56158)
                     /locus_tag="ACHA6B_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947814.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(56271..56906)
                     /locus_tag="ACHA6B_000206"
     CDS             complement(56271..56906)
                     /locus_tag="ACHA6B_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8947815.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(56940..57401)
                     /locus_tag="ACHA6B_000207"
     CDS             complement(56940..57401)
                     /locus_tag="ACHA6B_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8947816.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(57531..57962)
                     /locus_tag="ACHA6B_000208"
     CDS             complement(57531..57962)
                     /locus_tag="ACHA6B_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8947817.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(57980..58300)
                     /locus_tag="ACHA6B_000209"
     CDS             complement(57980..58300)
                     /locus_tag="ACHA6B_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8947818.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            58599..59375
                     /locus_tag="ACHA6B_000210"
     CDS             58599..59375
                     /locus_tag="ACHA6B_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8947819.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            59390..59593
                     /locus_tag="ACHA6B_000211"
     CDS             59390..59593
                     /locus_tag="ACHA6B_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8947820.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            59609..59929
                     /locus_tag="ACHA6B_000212"
     CDS             59609..59929
                     /locus_tag="ACHA6B_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8947821.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            59934..60104
                     /locus_tag="ACHA6B_000213"
     CDS             59934..60104
                     /locus_tag="ACHA6B_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947822.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(60111..60893)
                     /locus_tag="ACHA6B_000214"
     CDS             complement(60111..60893)
                     /locus_tag="ACHA6B_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947823.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            60959..61651
                     /locus_tag="ACHA6B_000215"
     CDS             60959..61651
                     /locus_tag="ACHA6B_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8947824.1"
                     /translation="MLKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQKYADLF
                     WEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEKAIRKN
                     TAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVLGGKES
                     RAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQL
                     NRQQRLFGDENE"
     gene            61829..62170
                     /locus_tag="ACHA6B_000216"
     CDS             61829..62170
                     /locus_tag="ACHA6B_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947825.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            62163..62834
                     /locus_tag="ACHA6B_000217"
     CDS             62163..62834
                     /locus_tag="ACHA6B_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8947826.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            62840..63526
                     /locus_tag="ACHA6B_000218"
     CDS             62840..63526
                     /locus_tag="ACHA6B_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8947827.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT        21058 a        10682 c        13304 g        18501 t
ORIGIN      
        1 catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga aaagaggaag
       61 aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa tttttttata
      121 tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt tttaaaatga
      181 gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa ggagtgtaca
      241 atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca tctaaagaaa
      301 tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt ttgactagcg
      361 atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt tctattttct
      421 tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa atattacttt
      481 atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg aaaaaggagt
      541 tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag gttatgatct
      601 ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag cattgccttt
      661 agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga ttacggctat
      721 cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat caggtccgac
      781 aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc aaggtgtatt
      841 gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc gattaggaac
      901 actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta ttgctatcat
      961 tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta caaacgttat
     1021 tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct ctttgatcat
     1081 gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga aacgaatgcc
     1141 tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc atttaccagt
     1201 agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga atatgggaga
     1261 ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg ctttattagg
     1321 aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata agcccttaga
     1381 tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt tctttggcgg
     1441 gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg gtgcacaaac
     1501 tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg tttttgcgcc
     1561 agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga cggcttggcg
     1621 tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt cagcagttct
     1681 attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg ctatcgtgat
     1741 cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca cgatttcatt
     1801 agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga ataactggac
     1861 agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca aaaataaatt
     1921 ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac caagtattga
     1981 tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa gaggacaaac
     2041 gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact tgtgggaaca
     2101 tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac atgcgaaata
     2161 tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa ctgaagattt
     2221 tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac ttgccagtct
     2281 tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg aaaaagtgga
     2341 agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt taattgtaag
     2401 acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt gccaagtaaa
     2461 cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt catcagttcg
     2521 taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg atttggataa
     2581 acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata cgggcggctc
     2641 cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga aagtgcctgc
     2701 atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc tgttttccct
     2761 ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc gataaatcct
     2821 tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt tgggtattcc
     2881 atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg cgactatttt
     2941 atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt gttttacttt
     3001 ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac tgttgtaatt
     3061 gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat gctgtcactt
     3121 cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg caattattaa
     3181 aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg aaaaacttga
     3241 cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg gagaatctac
     3301 aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg aaaaacacgg
     3361 caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac accgtaaaca
     3421 aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt aatagcgttt
     3481 gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc aggtcagatc
     3541 gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga tatcgtttgt
     3601 gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt agccggtttt
     3661 gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga agtcacttca
     3721 ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct tttattagga
     3781 ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat taatgataaa
     3841 taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca caaaaaaggt
     3901 ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc taaacgtgct
     3961 gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac aaaaatctat
     4021 ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt tgatggcgta
     4081 gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc agtagctcaa
     4141 gaagcttaat tgaatcttat gattaaagag gttgtgacaa gacttttgtc acagcctttt
     4201 ttgtttataa aaataaaagt tgtagtggag gctacgaagt ttactttgtc tttttacact
     4261 taatattttt ttgccaattt ggaatcagga tgttttgata actatcgttt tcaattgatg
     4321 gaagctatat tgtttgctat cagtgaaata attatatggt aaacagtgtt ctttttcttg
     4381 tttccattat aaaaatcgta tcgggatttt tcgttctttt aaattgaaca tttctatcta
     4441 tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg acgaaacaga aataaataca
     4501 tagtattatg ttttgaagcc taagtacaat agtctataga aaaggagcga taccattggc
     4561 aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa accgctcatt taacaaaggc
     4621 agaatatgat caaggagtaa caacacagga aattgctgaa gtgttaggga ttcagcgaac
     4681 aaatagcagt aaagatctga atcagctagt gcgcgaagga aaactgataa agacagacgg
     4741 acgaccagta cgttacatat atcaaacact cgttacgcac gtaaagccag ttacaaaaca
     4801 tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc aaatagatac
     4861 taaggatatc tttgcaaaaa tcattggagc taacggcagc atgaaaaatt cagtggaaca
     4921 ggcaaaagct gcgattttgt atccgccaag aggattaaat tgtttgatta caggacctac
     4981 cggatcagga aagacttatt ttgcacacgc gatgtatcat tttgcaaaag cgcatcatgt
     5041 aatagatgag gaaacggaac tgattgtttt caattgtgct gattatgcca ataaccctga
     5101 gcttttgatg agtcatttat ttgggtacgc aaaaggtgct tttacaggcg cagatgaaga
     5161 aaaaatgggg attatcgatc aagcggatgg cggtatgctg tttttggatg agattcaccg
     5221 gttgccgcct gaaggtcaag aaatgatctt ttactttatg gatcatggaa cttacagtcg
     5281 tcttggggaa acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg cgaccacaga
     5341 agacccaggt tcttctctat tagaaacttt tgttcggcgt atcccaatca atatcaaact
     5401 tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa tcatgattgc
     5461 ccacgaagca aaccgcacgc agcgaaagat ttctttgaca gaagatgtag caaaagcatt
     5521 gatcggcagt gtgacctatg gaaatatagg acaattgaag tcaaatatcc agttagtatg
     5581 tgcacgagga tttctaaatc atatgcaatc acccgaaatc tcgatcacaa tcgatgattt
     5641 gacagaaggg attcgtagcg gattgattca attagcaagc aaccgcagtg caatggcaga
     5701 gctttctaaa atattggagc caaagataac tgtagcacca aatgatacga tcatgaaaat
     5761 ccaatcggac tcctatgagt taccttataa tctatatgat atcatcggag ataaagcagc
     5821 tttactgaag tcggatggtt tggatcaaga agcaatcaat catttcattt ctacagatat
     5881 caacatccat ctgaagtctt tctataaaga ccatggtttt tcttttaatg cagacagcaa
     5941 attagcagaa tttgtagatc cgaaaatcat agaagtgacc aatcagattt atgtaatggt
     6001 caaaagatct ttaccatatg agtttcagca aaactttatt tatgctatga gcttgcatat
     6061 cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg ataacatccg
     6121 tgagatggct tttgattttc cagatgaata tgctgttgcg aaagaagtgc gtagttatat
     6181 cgaaacgtat tttcaagtgg agattccaga aagtgaagac tattacttga ccgttctact
     6241 cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg cagctcacgg
     6301 aaacagcaca gcaagcagta tggtccaagt tgtccaacag ttgttagatg cagagaacgt
     6361 acgagcagta gacatgcctt tggatatgga tccgaaaaca gcattggacc gaattgaacg
     6421 caatgtacaa gaagtagatg aaggaagcgg ggtcatactc ttagtagata tggggtccct
     6481 tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta ctgttgatat
     6541 ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag ggacagatct
     6601 agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat cttctgtaga
     6661 atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta tctgcgcatc
     6721 tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa acaagcgtca
     6781 agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag aactgccagt
     6841 tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga atatccaagc
     6901 cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt tggatcagtt
     6961 gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag agtcagctaa
     7021 aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta gtaagttaat
     7081 cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg gcgatgaaga
     7141 atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac gtattattcg
     7201 gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg atcctcttta
     7261 tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa aagtgccgat
     7321 agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa aagaatacac
     7381 tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg tgtattggtt
     7441 aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt ttgcttatag
     7501 tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg tgaagtagtg
     7561 aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat cgtttaatcc
     7621 acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc attgtgatca
     7681 gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct gcaccgccgg
     7741 ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat agtggattat
     7801 tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa gcggttgtcc
     7861 gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact gaaggcaaat
     7921 caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt cgttacttga
     7981 acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa atccttttga
     8041 tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa aaggtcaaat
     8101 aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa caaaagtaaa
     8161 taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt gctgaaggaa
     8221 tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc gtgattttga
     8281 aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt gcttcttttg
     8341 ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg ttcaatcaat
     8401 caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt ttgaatttac
     8461 caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat gaaattgcag
     8521 cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa ttagaaccgg
     8581 cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt ccagaaggaa
     8641 ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca cgtctgttac
     8701 atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt atcgttgttt
     8761 cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct gctcctccag
     8821 gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa gaccctcgtt
     8881 ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg acagctgttg
     8941 aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg gtcggtaaag
     9001 tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt gagaagcttg
     9061 aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc gataacatgg
     9121 atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata atattaaaat
     9181 ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag ttgcctttct
     9241 agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag tggcatgtac
     9301 attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg gtacgcttca
     9361 gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg ctgcattagc
     9421 atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag gtgttccttc
     9481 tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa tgatcgtacg
     9541 tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag gaaacatccg
     9601 taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta tcgcggtccc
     9661 agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg aatcaatgcc
     9721 agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg taggttacgc
     9781 tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta ttggtttcgt
     9841 tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg ttgcattagc
     9901 tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag gcggaagcaa
     9961 ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga ggaaaataaa
    10021 atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc gtggcgctct
    10081 accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg ggcattttct
    10141 atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc agcattaaaa
    10201 cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt aggggttaca
    10261 ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc gattcaaggg
    10321 gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt ctggtttaca
    10381 gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa tatccttgga
    10441 ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg gtatacacaa
    10501 gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg attgttacaa
    10561 gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct tgtacaaaga
    10621 tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa aggtgcttac
    10681 atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt tgaacaagtt
    10741 aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa cttagaccaa
    10801 ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt gcttaagaaa
    10861 aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt tggacacgtc
    10921 atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct ttttttatat
    10981 agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa tcaatacaaa
    11041 agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg ggcaaatcat
    11101 gataggtgat aatggttttg agttttacca tgcgcgagat agacgcaaat atatccaaat
    11161 tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa aatggattcc
    11221 tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca aagaacctaa
    11281 aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac aatcattgag
    11341 tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt aaaaaataaa
    11401 gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa atatgaaata
    11461 gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta gcaaagaagg
    11521 aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg gagcaattgc
    11581 aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca ttgctggact
    11641 agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag ctgcaggtat
    11701 ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct catttttctt
    11761 tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca gcgagactgt
    11821 gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa tttttattta
    11881 actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag aaagagggag
    11941 tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga
    12001 ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta caggtacgac
    12061 aggattagct gttattactt tggaaaaagc attttttatt actgattttc gttataccga
    12121 gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac ctatttttga
    12181 tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg aggaaacaac
    12241 agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat tagtacctgt
    12301 ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg ctattatcga
    12361 aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga tccaaccggg
    12421 catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc ttggcgcttc
    12481 aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc cgcatggtgt
    12541 agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg gttgctatta
    12601 cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg gggaaaaatt
    12661 aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg ctgcaaaacc
    12721 aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa aacatggtta
    12781 cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc acgaaggacc
    12841 aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc
    12901 agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt tgatcacaga
    12961 agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat agtttcatca
    13021 atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa atcactcgat
    13081 ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt gggtgttttc
    13141 tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt gatcactatg
    13201 ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa taaataaatg
    13261 gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat cgtgatcgct
    13321 cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg cgtatacgga
    13381 atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc tcacggaaaa
    13441 ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg ttatctagaa
    13501 tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc
    13561 cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc tgttgttcct
    13621 gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga agagaatgaa
    13681 taaagaatta tcaagacacg agattcggga aatggcattg caggctttat ttccattaga
    13741 cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat tagaccaccg
    13801 cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata cgctagttgg
    13861 cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt taaaaaacaa
    13921 ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg cgatttttga
    13981 aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa tcgagttggc
    14041 aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt
    14101 gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat cataaacctg
    14161 ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg ggagtggtta
    14221 gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag agattgctga
    14281 aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt tattggtagg
    14341 ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg
    14401 aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac tacttgcaga
    14461 aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat taccattgcc
    14521 aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag acgtagacgg
    14581 ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga ttccttgtac
    14641 tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg gtaaacgagc
    14701 agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac tcttgatggc
    14761 agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac tggcacaaga
    14821 agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag aattcgtaaa
    14881 accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta agctgatcgg
    14941 tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg tgccaaaggg
    15001 tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt
    15061 tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa cgaagtattt
    15121 gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag gcgaaatatc
    15181 gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg accatgcaaa
    15241 aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc caaaagaagg
    15301 aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt cttatcaaat
    15361 ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat tggaagaacg
    15421 aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt
    15481 cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg atatcatcac
    15541 gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag ttgtacaagg
    15601 aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga tgggcaattt
    15661 cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc cgtttaatga
    15721 agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat ctgtaggaca
    15781 tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca ctccaacagc
    15841 agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc aagaacggca
    15901 agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg
    15961 tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa
    16021 attagaccag ttgaaacagc gattatatca agctacacag caaatttatc atgcgaaaga
    16081 aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt atcgagttaa
    16141 agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa tccaacaata
    16201 tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt tgttgagtcc
    16261 attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag tagtaaaaag
    16321 tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg gaatcgttaa
    16381 aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca acttttgaag
    16441 agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac gttccattag
    16501 aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc caagatacat
    16561 tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt
    16621 ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat tcatctacct
    16681 gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag aggactctat
    16741 gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt attactttta
    16801 acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt agcagctgct
    16861 ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat ggacaatgat
    16921 gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt agctatattg
    16981 gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca cttaggtaat
    17041 tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca aggtatggtt
    17101 gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga agaattagca
    17161 tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc tggtggcata
    17221 ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga acatttaggt
    17281 ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa aacattaggg
    17341 aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt attaggccta
    17401 gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat catagacaaa
    17461 ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat caagcaattt
    17521 gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta gcggtcaaac
    17581 aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt ttgatttaca
    17641 acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt agtgaattaa
    17701 gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta gaaaaagcac
    17761 tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt gcatccactg
    17821 gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg
    17881 gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta atggaaagag
    17941 tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa gttgctgtta
    18001 tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct attttaaaag
    18061 aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga gaagctgtag
    18121 ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa gacattttgt
    18181 catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg atcacggggg
    18241 gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc ggaacaatca
    18301 attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt gaccataaat
    18361 aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt ataccaagga
    18421 aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa
    18481 gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa gcaggaagaa
    18541 tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat ttccagagat
    18601 ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg ttatagcata
    18661 ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa agacgctttc
    18721 gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg aaatgcttcg
    18781 gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat tatcaatgat
    18841 gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt gaagaaagac
    18901 ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc agtattacta
    18961 actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc gcgctgaccg
    19021 gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta gcaggaggaa
    19081 gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg
    19141 aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc gaaacagatg
    19201 aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga
    19261 atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta gtagatatcc
    19321 aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct ttattggatc
    19381 gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa tatatctctt
    19441 atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga
    19501 gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg caagtcggag
    19561 aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg
    19621 gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta gatggtgttg
    19681 gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat gaagcaattt
    19741 atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat atgagtcgtc
    19801 agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa cgtttagaat
    19861 taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg gcttactatg
    19921 acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg gacaaaatga
    19981 aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat cttcatgaag
    20041 cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg aaaagtctgt
    20101 atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga caattgaatc
    20161 atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag ccactgaagc
    20221 ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta
    20281 ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat acaggtgtga
    20341 gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa tactctcaag
    20401 ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa tattatattg
    20461 tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa
    20521 gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc acactcgaac
    20581 atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt
    20641 ccttttcagc agctgatagc tatattttat ttttacatga cactcattac agcagtcaat
    20701 acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt tactttagca
    20761 aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa ttagttgatt
    20821 caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa cttccctttt
    20881 tttaaataat aggtagttac gatacaaata aataatgtta atagaactag ccatataaag
    20941 gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact gccgacgatt
    21001 gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt attcagatta
    21061 ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa ttgaatgcga
    21121 gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt tttcaaagca
    21181 tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa attagcagaa
    21241 gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata aagttgtttg
    21301 ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg aagcgtagct
    21361 aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat attgagatta
    21421 ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa cagtggttca
    21481 tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag cgggtatgta
    21541 tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa caataataac
    21601 gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc tgcatcgtcc
    21661 aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg atgaatctct
    21721 tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt tccggtatta
    21781 cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct gttgcctgtt
    21841 tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa gatatagaat
    21901 ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca gtatacttga
    21961 agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta tcttgttttg
    22021 gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt
    22081 aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt ggggcggtat
    22141 gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct ttatttctgt
    22201 tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag aaattattta
    22261 tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc
    22321 tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa
    22381 gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt
    22441 tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag actaatttta
    22501 gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat aatattgatg
    22561 caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc
    22621 tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg
    22681 aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt gtgcgattct
    22741 tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat atacccagca
    22801 cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa
    22861 ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa gagaactttg
    22921 acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag gctgactaat
    22981 gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg gattgaacat
    23041 caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata gtgaatattt
    23101 attaagacaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc aaaaagcaat
    23161 cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca catttatcaa
    23221 agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg tagacggcat
    23281 tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg gatttagtta
    23341 tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat ccgcttatca
    23401 cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct atggcgagga
    23461 gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca
    23521 aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg caagaagaaa
    23581 aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa atgacgaact
    23641 cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca atggaagaat
    23701 cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt ttaaagaata
    23761 tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat tccaaccaga
    23821 actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa cagaaaacaa
    23881 tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag aggagtagaa
    23941 tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa ttggtggatc
    24001 agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt ccacaatcac
    24061 cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca agcgtggcga
    24121 ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc agcggagctg
    24181 aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt
    24241 tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc gcagaagaaa
    24301 aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata
    24361 aaatttggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc aaaaaagtag
    24421 gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc cgtttctcgt
    24481 atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca aaacagttgt
    24541 accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg
    24601 cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa acatatacct
    24661 ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca gaaatcctgc
    24721 ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa
    24781 aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa gaaaccaagc
    24841 aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc
    24901 aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac gcagttccag
    24961 acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac aatgatatct
    25021 tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat cctctgccag
    25081 gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg actttagaca
    25141 gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa tatcagccag
    25201 aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca atgggacaac
    25261 gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct atctggcgta
    25321 atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc actactgcag
    25381 ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga aaaatccaag
    25441 tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc
    25501 aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga ttaggcggac
    25561 gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct ggattagatg
    25621 atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg agtgcttatg
    25681 gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct attgcgaata
    25741 acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa actggagaag
    25801 agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca acagaaaaag
    25861 tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct tatggtgtat
    25921 atagtgtacc tggatacaat gtatcggcaa aaaccggaac agcccaaatc gcttctgata
    25981 cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag atggttcctt
    26041 ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa
    26101 tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt aaagaaactg
    26161 aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc
    26221 ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg
    26281 gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg atctctggag
    26341 aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa
    26401 aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt gatagagatg
    26461 gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac aaacttagtt
    26521 ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg gacacaagca
    26581 ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct atttattggt
    26641 tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa gtggcacaat
    26701 agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc tattttgaca
    26761 ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat cttgcttttt
    26821 gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat attcaagaaa
    26881 agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg gggaatcatc
    26941 ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt cggactagat
    27001 ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg attttcaaat
    27061 gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag tatctctttt
    27121 gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat catttgctta
    27181 agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc caagattttt
    27241 atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc tataatgcta
    27301 aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac agcaagtgtg
    27361 atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat gtctccaatt
    27421 catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt tttttggttg
    27481 atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat gacgggaaat
    27541 gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg
    27601 agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta atgatggaaa
    27661 accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa aagtaataac
    27721 aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca aaaatccagg
    27781 gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc cggtaattac
    27841 ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta caggaacgaa
    27901 cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag cagatcaagg
    27961 aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc aagaagcaaa
    28021 atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa tcacagattt
    28081 ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt atcatggtac
    28141 aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta
    28201 tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa caaaagcacg
    28261 cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg acgattactc
    28321 aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac caggtaagca
    28381 taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga
    28441 agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc aatatgtagg
    28501 agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt tagcgactaa
    28561 aatggctcta agtggatttg aaacgtctaa ggtcgtttta ttggctggcg gattagacag
    28621 aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa tggtcgtatt
    28681 tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt
    28741 aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta cagatggtga
    28801 tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt ttgaaactag
    28861 aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat
    28921 aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat ccagctcttg
    28981 cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat gtgggagcaa
    29041 aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat accttggaga
    29101 ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga ttgtttctta
    29161 agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc gttatcggta
    29221 ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt attccaacag
    29281 ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc cgatatgtgg
    29341 atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac aaagcagttc
    29401 ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat attttgaaaa
    29461 catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt caaggagcac
    29521 ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct tcttaccaag
    29581 ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata aatcctgaaa
    29641 tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg gcttccagtg
    29701 atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct ctggggttac
    29761 cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa aatgcaatga
    29821 gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac
    29881 taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag
    29941 cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta gtaaaacaaa
    30001 taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc
    30061 tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc gaaaatatct
    30121 ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat tagatacacc
    30181 cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc
    30241 cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg gaccatataa
    30301 cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag tactatttcg
    30361 tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga tctattatgt
    30421 ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag tcaacagcca
    30481 agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac agtattttca
    30541 tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa cagcaaatat
    30601 acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag agattgcttt
    30661 gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat tagatgaaaa
    30721 agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact ctgcaaaaat
    30781 caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag caatttctga
    30841 aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata tgaatgacgg
    30901 caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt attcgcaagt
    30961 agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct tctcgcatcc
    31021 ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga
    31081 agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac
    31141 acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg aaacaagtca
    31201 aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat aaaacaactt
    31261 taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg gaagcagtga
    31321 atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg tatatggaat
    31381 agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg ccttgatatt
    31441 ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat gaatattatt
    31501 ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga tatcgataaa
    31561 acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg tatccagatc
    31621 aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg ccagggaatg
    31681 attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa tgtggcttca
    31741 gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat
    31801 tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta
    31861 gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat acgtaaatgc
    31921 gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc gctgactgaa
    31981 tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat gggtggcggc
    32041 caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct tgaccaagaa
    32101 ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt taacaatgca
    32161 gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc agatgaagaa
    32221 tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca
    32281 gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc attggaccaa
    32341 atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc tagtcttcct
    32401 ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta tgttccaaat
    32461 cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga ttattcagca
    32521 aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac ctcagcagct
    32581 cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga agcacctgaa
    32641 gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc
    32701 aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag atatggaatt
    32761 ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg
    32821 cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg aattcattac
    32881 agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta tccaattagg
    32941 ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac aaaaagcggc
    33001 agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga ttttcatcac
    33061 tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa
    33121 agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa
    33181 acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt
    33241 aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga tgcttgaagc
    33301 tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg atttgatcac
    33361 agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg
    33421 tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag aagcaacgaa
    33481 aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac aagtacttct
    33541 taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt cagatatcgt
    33601 agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca atgaagaaat
    33661 gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg
    33721 caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa agactgtatt
    33781 agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg gcgattggga
    33841 tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg acaatatcga
    33901 aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt
    33961 aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg
    34021 acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt catcgtgatc
    34081 cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa gctgaagaat
    34141 tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta ttagaaaaga
    34201 aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat ttacagcgca
    34261 gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac agtttaagat
    34321 tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt gaagtaaatg
    34381 tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg tttgtggaat
    34441 ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg cctaaagaag
    34501 cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc
    34561 aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg agtcaagact
    34621 ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag
    34681 acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc taattttttc
    34741 ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac aaaaaaggct
    34801 gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt atctgctgcg
    34861 aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga aagtattcag
    34921 ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac tgtagcgaaa
    34981 gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg aaaaatcact
    35041 gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat cattgctgga
    35101 gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag aatcgttgat
    35161 tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg agatgagatt
    35221 tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc ttttgcagat
    35281 gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca ctattacttc
    35341 atttacttca acgggcagcc tatttctata gcattatctt gatggtatat gcattgctct
    35401 cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag atctgcgaac
    35461 cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat ttcacaattt
    35521 taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc gttatatgga
    35581 taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag aatagatagg
    35641 aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa cgtacattta
    35701 tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc tatctgaccg
    35761 aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa agcagtgatt
    35821 tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt ttgattttcc
    35881 ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa atccattatc
    35941 ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg ggaacgggga
    36001 tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa gtatttattg
    36061 caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag gttgctgtac
    36121 gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg acacaagaaa
    36181 gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat aatatttcca
    36241 gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg acagaaaata
    36301 caagacctga ttttgtactt gaactattag atattatttc agtcagaggt tatggcagag
    36361 tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta ctgttcggtg
    36421 tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat tagatatcca
    36481 aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag atgatttctt
    36541 agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat tagaaaaagc
    36601 agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg ctttgaacca
    36661 atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa gcaaggaatc
    36721 cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga acgctgaaaa
    36781 acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct tgacagatgc
    36841 aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa aaacacgagt
    36901 attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga aaagtccgga
    36961 atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg aagtgatcaa
    37021 agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact cagacacagg
    37081 agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct cgatcgtgcc
    37141 tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa gcgagccgat
    37201 gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga gacctgcttt
    37261 tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag agtttgcact
    37321 agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc ggctgaacat
    37381 gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac
    37441 cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc gagaaggcga
    37501 gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg
    37561 aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata tccatttagg
    37621 acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta
    37681 tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac aagtattggc
    37741 taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa aatgtcgtga
    37801 atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag gagttgcagg
    37861 agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac aagtacgcgt
    37921 atttggtaaa atggctgaaa aaagctatat ttacaaagga ttgaaaccaa tttactggtc
    37981 tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg taaaatctcc
    38041 atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg aagatacagc
    38101 ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta ttgctgtaaa
    38161 tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga
    38221 tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt
    38281 tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac gtgaatcact
    38341 tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc atactgctcc
    38401 aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg tagtgtcacc
    38461 tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta tcttttatga
    38521 caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga
    38581 tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga tctatcgggc
    38641 aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg atgaagtaga
    38701 aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga tccgtgaccg
    38761 cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga ttttctatgc
    38821 tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa aattatttgc
    38881 tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt
    38941 tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca tggatgtctg
    39001 gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat tatcattccc
    39061 agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact caagtatcac
    39121 gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc aaggatttgc
    39181 tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg caccagaaaa
    39241 agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg tagattatga
    39301 agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat atcgtaaaat
    39361 cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta aaaaagatac
    39421 tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat tgaaccaagt
    39481 aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata tttaccgtac
    39541 agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg ctaaagatgt
    39601 agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg ttttctatca
    39661 aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag cagaagaaat
    39721 ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc ctggttatga
    39781 agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg acttccgtga
    39841 taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta aatctttgga
    39901 agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag ctttggatgc
    39961 agatattgca caattattga tcgtttcacc agactacttt gaagttatgg cagcagatga
    40021 acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg aaaaagcaga
    40081 cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact
    40141 tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc
    40201 tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt tgtcacaccc
    40261 ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct ggttgccaga
    40321 tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc attgatccag
    40381 ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg acatccacga
    40441 tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca ccattcaacg
    40501 catctaatag taatttttcg taagcttctg ggctattttc aaccatttct gcactatttc
    40561 taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc ccattcaagg
    40621 ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt ggaagtgtcg
    40681 tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact tgtttgaata
    40741 cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa
    40801 cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt tcagtctctg
    40861 attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca tccaattcac
    40921 cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta taaatgcgga
    40981 tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat gctactggcg
    41041 gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg tctttaagtg
    41101 cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg ccaaaagtaa
    41161 tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta ttggcaaaac
    41221 ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc ctgaaaatat
    41281 cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac tggtaatcag
    41341 aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata atattttgtg
    41401 atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa taatagacac
    41461 ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt aatgtctgat
    41521 aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca aaggtagttg
    41581 cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca cggtagtgct
    41641 catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct aaatttcctt
    41701 ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca cctgtaccgc
    41761 cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc tttatatttt
    41821 atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc tatcattttg
    41881 gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt cgttgatatt
    41941 caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc gtgcgatacg
    42001 cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat ggacaacttc
    42061 ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca aatagttttc
    42121 taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat catcatggac
    42181 ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc gatgtttgcg
    42241 aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt gataagggga
    42301 atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag atgcagctga
    42361 gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc cacctagttc
    42421 taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc ctttcatggt
    42481 aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa
    42541 aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag agaagactca
    42601 ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt cagataacaa
    42661 cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag aaaagataag
    42721 ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta ccacagatgt
    42781 gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta attgttgttt
    42841 ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt ctgacttttc
    42901 cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga gtgaaccaag
    42961 aaaatagatc atttttttta tcaccaaatc acccctttat gagaaaataa tcgcatagac
    43021 atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc taagactttc
    43081 gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag gaagaaaggt
    43141 gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc ccgaatcggt
    43201 ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc aaaaatacag
    43261 gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt gctcctggac
    43321 ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac tggtttaata
    43381 tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc acgatccttt
    43441 taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat cttcactcca
    43501 aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa gtcaaaggta
    43561 tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc agaggattga
    43621 aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat gtcttgttta
    43681 ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa ccagcactag
    43741 ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat aattgtcaag
    43801 tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat atcccttttg
    43861 aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt atcggaacaa
    43921 attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat gaattgacag
    43981 cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat gaaggggata
    44041 tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac accatttata
    44101 ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg gcaggaaaaa
    44161 atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa atcatccatt
    44221 tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat gaatcaggtt
    44281 ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca aatttcagct
    44341 atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg aatatcttct
    44401 cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta tatgcagctg
    44461 ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt tctactggtg
    44521 tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg atccagccag
    44581 gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga acgctagtga
    44641 tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat aaagaaatca
    44701 ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag gattttgcca
    44761 atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag tggatcgagc
    44821 tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc atcaaattcg
    44881 atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc ccgaaaattg
    44941 gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac gagcagtttt
    45001 tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt caggtaaaac
    45061 actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa gtgcgttggt
    45121 catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac gtgaatgggg
    45181 aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca aacgtcagat
    45241 tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa tattggaatt
    45301 gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg atgaagcaga
    45361 ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc aagctccaac
    45421 agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa gcattgaaaa
    45481 aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt ctcgaaaagg
    45541 actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact tgagacgtct
    45601 cacgcatata gaagattttc atggtctgat tttctttaat caactgactg aattaggcgt
    45661 aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct ctgatcagaa
    45721 caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca gagcactttt
    45781 gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg tagtgaatgc
    45841 agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg gtcgaatggg
    45901 taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt taaaacggct
    45961 tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta agctgcaaac
    46021 tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg cgaaaacttt
    46081 ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa aagttaaaaa
    46141 taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt tttatgctat
    46201 gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa agaacgtcaa
    46261 gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag tgatgtagct
    46321 gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact atacaaggcc
    46381 catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac ggaaaagtca
    46441 aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac actcgttgga
    46501 gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct ttatccaccg
    46561 cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat gtttgctgaa
    46621 tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc atttgtttct
    46681 ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat ctttgggatc
    46741 aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc caaagcagac
    46801 ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca ggaaatgctg
    46861 tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca agtacatgaa
    46921 gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt atctacgttc
    46981 aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc actggatgag
    47041 cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa tcaacgtatc
    47101 gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg aaatatcggt
    47161 caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta ccgtactcat
    47221 catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca aaaaggatta
    47281 ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa aagtcagtat
    47341 ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga acgaaacatg
    47401 caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg tgtggagaac
    47461 attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac atatgtcaaa
    47521 gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg gcggttaacc
    47581 aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga aaaagctcgt
    47641 tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg tcatatgatc
    47701 gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca ggtagcaatc
    47761 gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga tgaaatcggc
    47821 tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa agaaaacaaa
    47881 gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt ggagatggca
    47941 caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt tatggaagtt
    48001 caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca tttgacgaat
    48061 ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt gatttatgaa
    48121 gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt gatagaagcg
    48181 atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat ccagctttcc
    48241 tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt acagcgtaaa
    48301 aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt atatcagcgt
    48361 attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt tttagaacgt
    48421 gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa agcaattgcg
    48481 ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga cgtttttgat
    48541 gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac agctaaaatt
    48601 gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact gatccgttca
    48661 ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga acctggagta
    48721 gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa acaagaaagc
    48781 catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga aatggatatc
    48841 gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc agaagatgat
    48901 atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac acactaatca
    48961 tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa aagcatgaaa
    49021 acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa agtgtaaaaa
    49081 aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt cagcagggat
    49141 gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag ggatggaagc
    49201 cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta agaatgtcga
    49261 tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac aaaaacttgc
    49321 gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga tgaacggaga
    49381 aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag ttgggggtat
    49441 aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat gtttggcggt
    49501 aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga ttttgagtta
    49561 gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca tgcacaaaca
    49621 gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt gactgttcac
    49681 agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga aatcatcgaa
    49741 ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga agaagaaaaa
    49801 tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa aaaacttata
    49861 ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc ctgccggttg
    49921 ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt atcggcacac
    49981 tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt cgtgatttac
    50041 cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga atctttgccg
    50101 tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta gcaatcgccg
    50161 gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga gtaaatgatc
    50221 tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt ggtccagcat
    50281 tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc aatagttctg
    50341 agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt tggctaaaac
    50401 tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct atttcagtca
    50461 ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat tcagtaccac
    50521 cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta tacgtcattg
    50581 ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt gatttggtgc
    50641 aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca gtattgatcg
    50701 tcacagtatt tgttcaagta ttctggttct ttggtattca tggaccaaat gtattggcac
    50761 cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt ttccaaaaag
    50821 gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat ggaaaagctt
    50881 atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca ggcggaacga
    50941 tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta acagtagcta
    51001 aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt ggtctgccaa
    51061 tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta gcaacagcaa
    51121 ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa gtcacatggg
    51181 tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg gcaccgatcg
    51241 taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc atctctgcaa
    51301 acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag cgggacgctt
    51361 taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac aattgccagt
    51421 gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga aggaatgact
    51481 tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc agaagatccg
    51541 gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg aacagtcctt
    51601 tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt tgagatgata
    51661 aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag tttgaatcca
    51721 cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac aggattagga
    51781 atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa taactaatgg
    51841 aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt atggactctg
    51901 tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag ctgaaaaatt
    51961 tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc gaagaagtca
    52021 ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc gtcgttcatt
    52081 atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa aaaatggagt
    52141 cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg atgtatttta
    52201 aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa atcaatgaaa
    52261 catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt cataggaaga
    52321 tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca aaatgacaaa
    52381 caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag ccacttatgc
    52441 atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg cggagaattc
    52501 ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca tggcgacccc
    52561 ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct atgttgcttc
    52621 ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga tccttgaaga
    52681 ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc ccttctatcg
    52741 gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca gtcgtgatgt
    52801 gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag atgcaaaagc
    52861 tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc tgaagcgcga
    52921 agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg ctttgcattt
    52981 tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt cttcctttat
    53041 tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc gcttcgtaag
    53101 ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg gtaatgatca
    53161 aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata tgatggcgag
    53221 gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac aagctgcatt
    53281 tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg tggaagtaac
    53341 aatcgaaaat ggagaaaatc atctctcaaa agtagaacaa acaaaactcc caatcgttcg
    53401 aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg aagagttggt
    53461 attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct caatcttgcc
    53521 acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg acaatatttt
    53581 tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag taaatcctct
    53641 tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt tttgtggtct
    53701 tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa gcgttctgcg
    53761 tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt atttcattct
    53821 acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact tataagctga
    53881 tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga tttattgaaa
    53941 ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt cgctggttca
    54001 attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg gtttgttttg
    54061 tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat cgaacacagt
    54121 attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat aagtagacat
    54181 tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg
    54241 ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt gcttttcaat
    54301 ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg aaactaatcc
    54361 attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat tctcgaataa
    54421 ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat caatggaaat
    54481 tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat aattccatgt
    54541 tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta atcctagaag
    54601 ttcagcaaat cgcattccag tggcaccagc aactaacacg agcataggag aagagtactg
    54661 cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat aatctaaata
    54721 tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc tagttggatc
    54781 aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat ggttattaaa
    54841 cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc gttgatatcc
    54901 cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt tcattaacgt
    54961 atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa gttgcatcca
    55021 atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc ctttgttcaa
    55081 gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa atcctgattt
    55141 tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt gccaactatt
    55201 tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat acaataggaa
    55261 ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt tgcttgccgg
    55321 cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta gatcatccaa
    55381 tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact tccagagttt
    55441 agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct gataggtcta
    55501 gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga attcctaaat
    55561 gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg gagctaatcg
    55621 ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt agctcttgtg
    55681 aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc attaagcatg
    55741 ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa tactcctcgt
    55801 tatcccatga agcgttgtta attcgaagta aacaattctt ataaagattt ttatacatat
    55861 ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg aatattccaa
    55921 tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg cgaatgtttt
    55981 ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc ttctcgtttt
    56041 tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct ccaataccgc
    56101 cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta ttatccatag
    56161 ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc actcttttgt
    56221 ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat ttatttttta
    56281 cttactttac tattaatcat atcccattga gcataggatt tttctatgtt cgatcctctt
    56341 gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc agcattgttt
    56401 aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc attccaactt
    56461 ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga ttcgataaca
    56521 gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt attttgctca
    56581 gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg attatttgtc
    56641 tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt agtagtttga
    56701 gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga tgaggaaagc
    56761 gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg tgttttctta
    56821 tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa taaaacaata
    56881 ccagtaaaac caaatatacc gtccattaat agatcctctt tctcttttga tattgaatat
    56941 tataaaccaa gcaattcttt tttcttaaaa tcgaattctt cttgattaat aatcccttca
    57001 tctaacaact ctttatactt tttaatttca tctgcacttg aaatttgtgt ttctttggaa
    57061 ttattatcta atatagattg ttgcttctct atatattttt ttaaattttc catttcattc
    57121 caatattttt ttgtaaacat aacggtgttt tcatctttcg tggccgctaa tatacctccg
    57181 cgactttcgt ttccgcctag caaggtaaat tgtatataac cattactcat acctggtttt
    57241 tttaattgta cggctgatat atttttaaag gggatagttt tttctccttt aataccttga
    57301 ttaactaaat ttataaatcc ttttctaata attgaaatag aatcatcagt tactttaatc
    57361 aaggtttttc ctggagattt tattaaaatc tctttttcca ttttttcttc ctcatttctg
    57421 tgatatgatt ttttttgtag aagctcatat atgaggaaag agcccgtgtt gcagcacggg
    57481 cttttttaat gttttgctga aatcggtttt tttaataatt cttgatgtat tcatacatat
    57541 tgccttgcgt aagaatattt tttcttaaaa tagcattggc agacagcata acaagtgtat
    57601 ctgagcttat tctgaataga ataatactcc ataaattttt cgagattaaa ctgagattca
    57661 tcagtcagtt cgttctcaat atagatattt aataaaatta gaatagctat tttatctgct
    57721 tctgtttcaa attttgagtg aaaagttgta gaagtatcgt acaaaactga aaattcaaaa
    57781 tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa aggcttctgc agtttcaccg
    57841 tatagatttt cattcaaaaa aatgattctg ggttttggat agtagaaacc aggctctttc
    57901 atctccatat aaactacttt caaattgtat tcgctcaaca tttctttcaa tttcaaatac
    57961 atacaaacca tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt
    58021 tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta tcgtcttctg
    58081 ataaatccac atgtttaggg gtttctcttt cttctctacc tagaaggtag tctacagaga
    58141 catcgaaata gtcagcaatc cgacttaatt catctgaatt aggagtatta tttttccact
    58201 ttgctagata accgttagaa tatccaaaat taatttctaa ctgccgtata gatatctttt
    58261 tcctcttcgc caagtctttt attatttcat aggtattcat cgataaaaca acctttctaa
    58321 atgtttacaa aaaaagttta gaaaaatacg cagaaataat ttgactaatt ctgagtaatg
    58381 agctatacta tatcttgtaa acagatttaa caactaaaaa gacaacaaaa aacactattg
    58441 atatgtaaat gcagaccgcc aagaaagctt taaaatcaat gtttttatgt cttatttaat
    58501 tatgctctta gtatagagta ttactcagat actgtcaatt gaatttagaa aaaagttgtt
    58561 aaatttgttt acgaatataa aagaaaggaa tgagaaatat gaacacaccg caaattttta
    58621 atttcgaaca aaacgaagtt cggacaattt tagtaaatga tgaaccatat tttgtaggca
    58681 aagacgttgc gagtgttttg ggttattcaa acactaaaga tgctttgtcg cggcatgtag
    58741 atttggaaga taagatgggg tcgcgaatca cgacctcagg tcaatcaaga gagatgacaa
    58801 tcatcaacga atctggtttg tacagtttaa tcttaaaatc aaaacttccc tctgccaaaa
    58861 aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat ggaggttatc
    58921 taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatt caattagcaa
    58981 ctcaactaaa agaagaaaga actggaagat taatcgcaga acaaaagatt gcagagtacg
    59041 aacctaaaat ctcctattta gatagcatat tatcttctac agattcagta acaattagtc
    59101 agattgcagc agattatggg atgtctccac aacagatgaa taaattactt cataaactag
    59161 gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg aaccaaggat
    59221 acacaaaatc tcatacaact gagatcccga aagccgatgg tggcactaaa attgtaatga
    59281 ataccaaatg gacacagaaa gggcgtctat ttatctacga attactaaaa aaagaaggat
    59341 attaccctca aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat
    59401 tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt
    59461 cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat
    59521 tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat
    59581 gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcaaaaa tcccaattcc
    59641 agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac
    59701 tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat atgattggtt
    59761 tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg agaaaaacaa
    59821 aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt taagaagtaa
    59881 aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggtaag cggatgggta
    59941 aatttaatag agcactagta ttcagcgcac cactaattat ctacgcttta ggtctttggg
    60001 gaagcagaca agcattaatc ggcacaattg tatatatggt ctggatcttt atggggcttg
    60061 atgaagctga atatagagcg aaaaagccag tcgggaggga ctgacttaac ctaattaatt
    60121 aaaaattgta acaaataacg caaaaataaa gctaatgaaa ttgtattgtt aggatctact
    60181 ccaatagtta tatttacaat ttcggttact actagtccta tcaaagctgc cccagtttgc
    60241 tctaaatacc acattttatc tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt
    60301 tcattagtta tatctttgtt cttggctgta tttatttctt caaaaagctt tatagaatca
    60361 tcaaggactt tcctttgctc attaaggata tcaaaaaact tagcctcaaa ctcatcagat
    60421 ggattagaag tgtctttaaa aagtagattt tttgtactaa cttccggttc ggaagaaaat
    60481 gctttttcta attcttgggt agctacccct attctgaagt cgtacttaga catgtcaacg
    60541 gcaaattttt ctgtgatttt agagatatca gaaaaataag gttttggcat agtagctgcc
    60601 atttttttag caatattaaa acttggagat tctaattgtt tctgtattgc gaacaaagaa
    60661 ggaggaatca tattagcaat agatttgtta cgtttctcga caatttgaat gagagaatca
    60721 ttgagtattg cttgcgaagc tataaaagta tcttgaattg caattaattt tttagcttgt
    60781 tctatcgcag gctgcatagc gttaacgaca tctagagaag gagcaatggt ttcaccgatt
    60841 aattttgaag aagctatcaa tttttgataa taaggagtat ctgaaatatc caattatata
    60901 caccaccttt ttacctaaat tataccaaaa gggagagaaa aaaatgcaat aattagtaat
    60961 tttgaaaaat aaagaagctg tgactacgag cttacaagtc gcagaaacat tccgaaagga
    61021 acatagagca gttttaaagg cgattgacga tctaaaagat gggcttgcac aaaagtatgc
    61081 agacctattt tgggaagatg tgtacatcca tccgcaaaac aaacaggctt atcgaattgt
    61141 ctatatgaat cgtgatggct ttactcttgc tgtattagga ttcaacaata ctcaaaaagt
    61201 tttagagttc aaattgaaat acattgaagc gttcaacgaa atggaaaagg caattcgcaa
    61261 aaatacagct tttgacacaa ttgaagacat gatgatctat caattagaag aaatgaaaga
    61321 tgttaaaaaa gatgtttcca tgcttaaaaa tactatgcga attagcggac aacaagagtt
    61381 tgaaattaag caaaaaggaa atacgaaagt tatggaagtt ctagggggaa aagaaagccg
    61441 agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac
    61501 cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt
    61561 tattgaaatg tggttaccag aaactgcgat tcgcatggaa atcgatcaac tgaacagaca
    61621 acaaagactt tttggtgatg aaaatgaata gagctgaagc gctaagaatc gggacggtaa
    61681 ttgctaatcg ctggtggaga cacaataaac caatcatcct aagccaacaa catattgaca
    61741 agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag agtcacaaag
    61801 aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc tagatcaata
    61861 tttgactgat cctagttggg gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag
    61921 acgaaatctt ttgacggatg aagaactagc atgtgatcaa gacgatttgg gaaattttgt
    61981 gagtatttgg gatcatgtct atcttatcca tttatcgagg aagtccaaaa aacctgaata
    62041 tatctatgtc atcgaagatg gcttgattga tgcgctagaa gagtatgaca gagataactt
    62101 gattgatatc tcttattacg gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt
    62161 tgatgagtga aaacaaaggg acaacgactt ttgaaaaact ttttagtcgc aagttaaata
    62221 aaatactcaa gaaaaaagga aattttgatt atttatcttg ggctcacgca tgggagatta
    62281 tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtataaacac tacagagttg
    62341 tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt atggatgaaa
    62401 ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc gaattatttc
    62461 ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa atcaataact
    62521 cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta tatgtatttc
    62581 aaggggaaga tattccaaca ccacctagaa tcgatacgaa gaaattaaac atgctagaga
    62641 cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat
    62701 atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa actattgaac
    62761 agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag agaaaagaat
    62821 tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag cggttctgaa
    62881 aaagttcaaa ggaaaagaca taggtgcaac catcaatgag gaagtgaaca ttgatcgact
    62941 aaagacgatg tatgacggct acgatggtga tcgagttatt gaaattcgtt ttattgatcc
    63001 tagacgtttc acagtacagc aacgaaactt catctatgca ctcataggcg atattttcat
    63061 cgatacaggc atgccaacgg acttctggaa ggaattcttt tacttccgct ttgaaggtgt
    63121 cacagggcgc aaaataagcc tcaaagatga atcgaatacg actgtgagtg atgctaacgt
    63181 cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca aagaaggcta
    63241 tgagatttta ccagcgaatc aagaatatta cttctacaaa tgcatcacaa aaagagtctg
    63301 ttgcatctgt ggcaaaacag gagctgatat cgatcacttt gacaaagcgc taggaagacg
    63361 aaagcgcaaa gaagttgatc attcagagta cacatttgca gcactctgca gaatccatca
    63421 cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat
    63481 caagttaaac caggaaacaa ttaagaaact tagaatagga ggataaaaaa tattggctga
    63541 caaca
//
LOCUS       DAYYHB010000004        61082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000004 DAYYHB010000000
VERSION     DAYYHB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.004"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..425
                     /locus_tag="ACHA6B_000219"
     CDS             <1..425
                     /locus_tag="ACHA6B_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947828.1"
                     /translation="LYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMAE
                     DMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFRF
                     VYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(655..903)
                     /locus_tag="ACHA6B_000220"
     CDS             complement(655..903)
                     /locus_tag="ACHA6B_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8947829.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(1002..1316)
                     /locus_tag="ACHA6B_000221"
     CDS             complement(1002..1316)
                     /locus_tag="ACHA6B_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947830.1"
                     /translation="MTEENKVDLLETLVINKKEFEQLFLDFFVQYDRIYDFNSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(1393..1887)
                     /locus_tag="ACHA6B_000222"
     CDS             complement(1393..1887)
                     /locus_tag="ACHA6B_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947831.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(1880..2182)
                     /locus_tag="ACHA6B_000223"
     CDS             complement(1880..2182)
                     /locus_tag="ACHA6B_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947832.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(2205..3542)
                     /locus_tag="ACHA6B_000224"
     CDS             complement(2205..3542)
                     /locus_tag="ACHA6B_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8947833.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQLTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEGELILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSKEVLTEIAIRHL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(3527..4024)
                     /locus_tag="ACHA6B_000225"
     CDS             complement(3527..4024)
                     /locus_tag="ACHA6B_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947834.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(4173..4511)
                     /locus_tag="ACHA6B_000226"
     CDS             complement(4173..4511)
                     /locus_tag="ACHA6B_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947835.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(4515..4700)
                     /locus_tag="ACHA6B_000227"
     CDS             complement(4515..4700)
                     /locus_tag="ACHA6B_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947836.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     MMKNELMNELSHIQKGA"
     gene            complement(4714..7266)
                     /locus_tag="ACHA6B_000228"
     CDS             complement(4714..7266)
                     /locus_tag="ACHA6B_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8947837.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLNTVVIGLMVLSLVIIGYKMVTGKGTVDLKSVGMNVVMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNSGGNDQTEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMAS
                     ERATKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPI
                     RLSDRKDGAKIENADRIMSSSERMREAMKNIAESNTNYASAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKSVKENLQNSTSSQDRRSVDVRENLHNTTSSQSGNRTVDVRENVQSSSTGQT
                     RTVDVRLNLQDTTSSQSGNRTVDVRENVQSSAPNQPKTVEVRETRAEASQGDKTQKIN
                     IVEERKERPNEKVIVEQEVKEKKRQRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(7253..7471)
                     /locus_tag="ACHA6B_000229"
     CDS             complement(7253..7471)
                     /locus_tag="ACHA6B_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947838.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(7464..8222)
                     /locus_tag="ACHA6B_000230"
     CDS             complement(7464..8222)
                     /locus_tag="ACHA6B_000230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947839.1"
                     /translation="MMELIILREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYEN
                     ATFMLSNLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGI
                     NYKIERPSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSV
                     PRYFKLKEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEF
                     YNGLVSNKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(8337..9212)
                     /locus_tag="ACHA6B_000231"
     CDS             complement(8337..9212)
                     /locus_tag="ACHA6B_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HFM8947840.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIAKQE
                     KASRLDMLETLKKIG"
     gene            complement(9382..9663)
                     /locus_tag="ACHA6B_000232"
     CDS             complement(9382..9663)
                     /locus_tag="ACHA6B_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947841.1"
                     /translation="MEQIGVSVMLNTDQTNQLKTHVFEVTKEAIDQARIDSGLERPFL
                     KGKEMAKFLNVSYGTFLKFKRLGLPIIVVDTIELYSKEEVKKWMLQHQI"
     gene            complement(9718..9915)
                     /locus_tag="ACHA6B_000233"
     CDS             complement(9718..9915)
                     /locus_tag="ACHA6B_000233"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8947842.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            10082..10456
                     /locus_tag="ACHA6B_000234"
     CDS             10082..10456
                     /locus_tag="ACHA6B_000234"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8947843.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            10546..11712
                     /locus_tag="ACHA6B_000235"
     CDS             10546..11712
                     /locus_tag="ACHA6B_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8947844.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(11811..12203)
                     /gene="rpsI"
                     /locus_tag="ACHA6B_000236"
     CDS             complement(11811..12203)
                     /gene="rpsI"
                     /locus_tag="ACHA6B_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8947845.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12219..12647)
                     /gene="rplM"
                     /locus_tag="ACHA6B_000237"
     CDS             complement(12219..12647)
                     /gene="rplM"
                     /locus_tag="ACHA6B_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8947846.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13181..13648)
                     /locus_tag="ACHA6B_000238"
     CDS             complement(13181..13648)
                     /locus_tag="ACHA6B_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8947847.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(13768..15306)
                     /locus_tag="ACHA6B_000239"
     CDS             complement(13768..15306)
                     /locus_tag="ACHA6B_000239"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8947848.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15386..16072
                     /locus_tag="ACHA6B_000240"
     CDS             15386..16072
                     /locus_tag="ACHA6B_000240"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8947849.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16273..16800)
                     /gene="msrA"
                     /locus_tag="ACHA6B_000241"
     CDS             complement(16273..16800)
                     /gene="msrA"
                     /locus_tag="ACHA6B_000241"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8947850.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(16884..17561)
                     /gene="pgmB"
                     /locus_tag="ACHA6B_000242"
     CDS             complement(16884..17561)
                     /gene="pgmB"
                     /locus_tag="ACHA6B_000242"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8947851.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(17558..18688)
                     /locus_tag="ACHA6B_000243"
     CDS             complement(17558..18688)
                     /locus_tag="ACHA6B_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8947852.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(18688..20871)
                     /locus_tag="ACHA6B_000244"
     CDS             complement(18688..20871)
                     /locus_tag="ACHA6B_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8947853.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(20868..21893)
                     /locus_tag="ACHA6B_000245"
     CDS             complement(20868..21893)
                     /locus_tag="ACHA6B_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8947854.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(21938..22765)
                     /locus_tag="ACHA6B_000246"
     CDS             complement(21938..22765)
                     /locus_tag="ACHA6B_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8947855.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(22762..23829)
                     /locus_tag="ACHA6B_000247"
     CDS             complement(22762..23829)
                     /locus_tag="ACHA6B_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8947856.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(23855..24691)
                     /locus_tag="ACHA6B_000248"
     CDS             complement(23855..24691)
                     /locus_tag="ACHA6B_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947857.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(24688..25557)
                     /locus_tag="ACHA6B_000249"
     CDS             complement(24688..25557)
                     /locus_tag="ACHA6B_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8947858.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(25627..26910)
                     /locus_tag="ACHA6B_000250"
     CDS             complement(25627..26910)
                     /locus_tag="ACHA6B_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8947859.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(26924..28609)
                     /locus_tag="ACHA6B_000251"
     CDS             complement(26924..28609)
                     /locus_tag="ACHA6B_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8947860.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            28884..29909
                     /locus_tag="ACHA6B_000252"
     CDS             28884..29909
                     /locus_tag="ACHA6B_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8947861.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(30504..34157)
                     /gene="rpoC"
                     /locus_tag="ACHA6B_000253"
     CDS             complement(30504..34157)
                     /gene="rpoC"
                     /locus_tag="ACHA6B_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8947862.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(34236..37853)
                     /gene="rpoB"
                     /locus_tag="ACHA6B_000254"
     CDS             complement(34236..37853)
                     /gene="rpoB"
                     /locus_tag="ACHA6B_000254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8947863.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            38581..39549
                     /locus_tag="ACHA6B_000255"
     CDS             38581..39549
                     /locus_tag="ACHA6B_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8947864.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            39567..40472
                     /locus_tag="ACHA6B_000256"
     CDS             39567..40472
                     /locus_tag="ACHA6B_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8947865.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            40469..41275
                     /locus_tag="ACHA6B_000257"
     CDS             40469..41275
                     /locus_tag="ACHA6B_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8947866.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            41432..42400
                     /locus_tag="ACHA6B_000258"
     CDS             41432..42400
                     /locus_tag="ACHA6B_000258"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8947867.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(42717..43241)
                     /locus_tag="ACHA6B_000259"
     CDS             complement(42717..43241)
                     /locus_tag="ACHA6B_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8947868.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            43528..45339
                     /gene="scm"
                     /locus_tag="ACHA6B_000260"
     CDS             43528..45339
                     /gene="scm"
                     /locus_tag="ACHA6B_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8947869.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(45494..46480)
                     /locus_tag="ACHA6B_000261"
     CDS             complement(45494..46480)
                     /locus_tag="ACHA6B_000261"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8947870.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(46503..47069)
                     /locus_tag="ACHA6B_000262"
     CDS             complement(46503..47069)
                     /locus_tag="ACHA6B_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8947871.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(47083..47499)
                     /locus_tag="ACHA6B_000263"
     CDS             complement(47083..47499)
                     /locus_tag="ACHA6B_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8947872.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(47956..49890)
                     /locus_tag="ACHA6B_000264"
     CDS             complement(47956..49890)
                     /locus_tag="ACHA6B_000264"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8947873.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            50192..51127
                     /gene="pplA"
                     /locus_tag="ACHA6B_000265"
     CDS             50192..51127
                     /gene="pplA"
                     /locus_tag="ACHA6B_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8947874.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            51217..52320
                     /locus_tag="ACHA6B_000266"
     CDS             51217..52320
                     /locus_tag="ACHA6B_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8947875.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            52333..53286
                     /gene="menA"
                     /locus_tag="ACHA6B_000267"
     CDS             52333..53286
                     /gene="menA"
                     /locus_tag="ACHA6B_000267"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8947876.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(54100..55446)
                     /gene="gor"
                     /locus_tag="ACHA6B_000268"
     CDS             complement(54100..55446)
                     /gene="gor"
                     /locus_tag="ACHA6B_000268"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8947877.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(55580..56833)
                     /locus_tag="ACHA6B_000269"
     CDS             complement(55580..56833)
                     /locus_tag="ACHA6B_000269"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8947878.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(56839..57762)
                     /locus_tag="ACHA6B_000270"
     CDS             complement(56839..57762)
                     /locus_tag="ACHA6B_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8947879.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(57983..60475)
                     /locus_tag="ACHA6B_000271"
     CDS             complement(57983..60475)
                     /locus_tag="ACHA6B_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8947880.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(60482..60949)
                     /locus_tag="ACHA6B_000272"
     CDS             complement(60482..60949)
                     /locus_tag="ACHA6B_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8947881.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        17670 a        12821 c        10695 g        19896 t
ORIGIN      
        1 aactttatac tgaaaaagtt gaacaatttt ttaagtctgg tgccaataaa gacttttcaa
       61 aaataagcga ttattcggag aactttttta atgaaaccca agatgaaatc atgaactcaa
      121 cttttatggc tgaagatatg tacaatatga cattaaaaaa aataaaagct tacgaccatc
      181 cgttaaatct gacactaaat aatggactgc tttcttttgt attagcaggt tacgttgact
      241 atgaatattc accagattat ttggagaaac ctcttcaaca cgagaacaat gatattaatt
      301 ttagatttgt ctacgagtca aaaagtaaaa catggttagt tgatcaatta agcacgataa
      361 acacaagctt ccatgaatta cctaaacctg aaaatgaagt tgagatggtc attaaaatct
      421 tctaaaaaag aagcgttgaa ggagaaatat tcctttcaac gctctttttt atatgcttag
      481 aaacataaat aaattgtata ctttcttttt agcccctatt acttttcacg aaaaatatct
      541 ttttttcgac gctagcgttt ctctacttct aatactgtac acagtttttg tattattgaa
      601 aaattactca ttatttagca ttaatccaca tcgttttatc aatcgacctt catactaaac
      661 tactttaaag tcatcttcct tctcgttttt cattaaatca gacacataag cccatgtacc
      721 cgttcgttgt aaataaagtt cttcttgtaa caactcccat tttctcttag aaatcataat
      781 gacgccatcg ttcaatccat ttttagaatc cctagtaatt tctatttcag aatttgaatg
      841 aactacttct tgcaacactt tacctatatt ttctttgaag tttgttggag taatagtatc
      901 caaaatgaac acctctcttc tctatacctc tattatgaag cgcccatcaa ataaagcaag
      961 cgttgaggaa taattctatc aacacttgct ttattaagct ttcattttgt agtttgacta
     1021 aaaccaataa tcttatttct aaactctcga aaaacttctt gtggctgata atagtctgca
     1081 tctatttcaa atgtttctga gaataattca ttgaattttt ctactgaaat aatgatttta
     1141 ccattcttct ctcgactgtt ttctattatt tctgcttcta attcaaataa atctagtgac
     1201 gaattaaaat cataaatcct gtcatattga acaaaaaaat caagaaaaag ttgctcaaac
     1261 tctttcttat ttatcactag tgtttcaagc agatcaactt tattttcttc agtcattctt
     1321 ccaatttcct cactttcgtt ttcaaaaatc acaaaaatga tattaactta aaagccacta
     1381 acaaaataag acttacaaat taaagggtaa tccaacaaaa taaccattcg ctctacgtac
     1441 tacgatttgg tagtcctgcc tttcaatcgg taattcataa tagtaattta ctgtttctga
     1501 tttaccactt tcaatatact caggattatc caatatcata gcatactcat aaagctcttc
     1561 accatcctta ttttcaatcg accattgttg cggacgtatc ggattttctg tatcattttt
     1621 cattgtatag gtcacttgta atacacgatc atgctgtgca aattcatttc ttttttcggt
     1681 cagttcaata tttttaaatg ttagttcaac cccatctgcc tgataagttt cacctatttt
     1741 taaatctttt gttacaattt ttattggctt ttcttctgga aaatacctgg ctttaattgt
     1801 tagataactt ccaaaaagaa tcaatcctag tagcacaaaa aaaattcctt taacgagcca
     1861 ctgaaacggg aagtaaactt taaacatctt ttttctcctc cgttttgtat ttggttttta
     1921 gagaacacga taacttttga tcaataccat ccaacttttt cattatactt gattctagtt
     1981 ctgaaattgt ttgttcaagt ttaatgcttt caggatcact aaatttttta tttgattgac
     2041 tagccaacat tggtaaattt gaaaccatca cacggaacat atttgctttc gttccaagag
     2101 ttcctttatt cttctcataa aagctgtcaa acaatttttt tgtttccttt gaacaatata
     2161 ctttaatata cggatcagac attcttttcc ctctccctgt ttacttaaaa atccatatca
     2221 tattgatttt gttgtttttg ataatcaatt cctcttgctg tttcttcata ctctctccga
     2281 gctttttcaa tcgtaccatc atcaattgca cgattaaata aataaagaac acgttttgtt
     2341 tcttgaatat aacgcttctt aaaattacgt tttaaagcgg gttcagtatc ttgaatgtta
     2401 aattggtcat aatcagttga taaactctct gcgatatcat aaacaacttt tttatacacc
     2461 aaatttacac atttcttctt atattttaaa ggttcctctt ccactgcaaa tccaaagaat
     2521 tggtttttat tctcaccaaa aacatccaat aaattttcta ttctgtcctc tatttcaaat
     2581 ttatatttat cataaaattc ttcataatct tttgatgatc taagaaatgg tatattctca
     2641 ttatctaatt caaagcgttg cgctcgttct tgccaattaa atctactatc cgctctacca
     2701 tttaacgatt taccaagatg tcgaatagca atttcagtga gtacctcttt agattgaatt
     2761 tgctccgctt ttttattttc tgtgaacaaa tcatcaagat aattcatatc atagttttgc
     2821 cctaaacgct ttccgccaat ttttaagatt aactcgcctt cagaatctaa gagtttgtac
     2881 ccaatacgct gatttttggc aaaccggtaa gtctcaatag aatggttttt cttcaattcc
     2941 gcatgaaaat catcataatt tttcgcatac tgagcaactt cagaaacagc tagttttatt
     3001 ttttctcgat ttgaaatctt attttcagtt ctaaattcac gttctttcca attttcacga
     3061 tctgcaacta aatgattata cttatacata tcaagtgggt taattccatt atcaataagt
     3121 atattatcat tccaagcctg caaaactcga atatcattct ttcgaatatc cattttcttt
     3181 cctgttagct gatcgtttgg cgaaatacca aagtgattgt gaacatgccc tttgtctgtg
     3241 tgtgtgacaa ccaaaattga ctggttagga aacatttttt ctgcccactt aacacccaac
     3301 tcatgtgctt tttcagcgga aatatcttct agtggatcaa agctttgtgt aaaatgccaa
     3361 agctttattt tccctttatc accaaaaatt tcattggttc gtttaaattc ctcaatagca
     3421 tttaaatcat tcgatacgcc aattcctgtc acgagttcag aatcttcttg tttatttaac
     3481 atatatgaaa ttgtattatg aatactccta ctttttgaag caatttttat aatcgagccc
     3541 ataacttatt caactctttt tccaagcgat tcaatggatc gcttattgat tttatttgta
     3601 tcattaagtc atttttttca gcatatagcc gctttaatcg ctcttgctta gttgtaaaag
     3661 ataagtaaac actctgatgt tcttcttttt gtatctcggt atctttttta tttaactgaa
     3721 cacgcaaata atttgctgtt ttcactaatt gattcaaatt tcttcctatt gctagtaaat
     3781 gaaaattaat ttctttatat tgtcgatcgg tatattttag acttttccct gcgttgtcta
     3841 ctgcgatttt cttcacgtac attggaacag ataaaccaaa aatgtctgct gttttttcta
     3901 gctcttggta ctgataatcg tctactcgaa actttatcgg cctattttgt cgtcgactat
     3961 cttcattacg tttatatcgc tttcgccttt cttctaatgg tagtttactg acatcaattg
     4021 ccatgcgcac ttctcctttt caatattatt tttaagttca aaagaagcaa gcgaggggaa
     4081 agggaaccct tccccgcttg tcgagggagt aaactcccta cgagcaccct catgctccgc
     4141 attttatcga gattagatag ccgataaatc agctacctaa cactttcttc ttgcgcctct
     4201 tctcgataga aaattcgata cgctgtctct ccttttcttc tcaaaaattg ttggaatttc
     4261 gtttccatca attgatcgct tgcaagaaat tcgtgtacta gggtaaattc gctattcgcg
     4321 aaatttaccg ccttttgcca tttttcttga gtaccttttt gattaatttc aaaagtttct
     4381 tcaaactctt ttactgatga aattcctgtt ttttcataga catactttcc cagttcggga
     4441 taaagttttc tctccaactc ggcgagttct tttttcaaat ctgcaattga cttttgtttt
     4501 ttccttgcca tttttcatgc tcctttttgt atgtgactaa gttcattcat aagctcattt
     4561 ttcatcatta attcttcttg aatcatcagt tcaatataat ttgtgaccgt gattttttca
     4621 tttgttgctt tctttactga ttcacataat tcgtcaagtt ctaagaaatc atgtgtacta
     4681 atctgtagaa ttaattgcat gacaatcacc tctttatttt ttaaatagac tatcaaacag
     4741 cggatttggt ttatcagaaa tatcctgaaa cagtggatta ttttcataag taaatcgttg
     4801 acgtttcttc tccttcactt cttgttctac aatcactttt tcgttaggtc tttcttttcg
     4861 ttcctcgaca atatttattt tttgcgtctt atctccctga cttgcttcag ctcttgtttc
     4921 acgtacttct accgtttttg gttgatttgg cgcactactt tgtacatttt ctcgtacgtc
     4981 tactgttcga tttccacttt gactacttgt agtatcctgt aagtttaatc gaacatctac
     5041 cgttcttgtt tgacctgtac tgctactttg cacattctct ctgacatcca ctgttcgatt
     5101 tccactttga ctacttgtag tattatgcaa gttttctcga acatctactg agcgcctatc
     5161 ttgactactt gtagaatttt gtagattttc tttaaccgat ttttctaatc cggtatttga
     5221 atttttagct tcttggattc tttctttggt caacgcttct ttttcgtcca tattagctcc
     5281 tgtacgccct tctacacgtt tccttaacat ttcctcacga ttcattgctt caggcgtgta
     5341 ttttgcttcc tgtcgcttct gattaattaa ttcttcagca gacttagctt gaccgtgcat
     5401 agcattttta cgttcttcaa tatcttgttg aactttttgc tgaattgcac ttgcataatt
     5461 agtatttgat tcagcaatat ttttcattgc ttcacgcata cgttcagaac tagacataat
     5521 tctatcggca ttctcaatct tagccccatc ttttctatcc gaaagacgta ttggcataga
     5581 atcagcatat tttccagttt catcttcttt gtttttagct actataggtt tgctattttt
     5641 attaatagcg gacacggtac catcatctaa accttctttg aatttattct tggtatcatt
     5701 agccatatca cgcatactct taaatggttt tgttgctctt tcactagcca ttgtagctcc
     5761 atccgaagct aagcctgata atccacgttc acgagcatac ccaagcgttt tccccgtttt
     5821 attagctttt tgagaaagtg attgaccaaa gttgcgttca ggttttcgtt cttccccttc
     5881 cgtttgatcg tttccaccac tatttttccc acctttagat cggttcatgc cacctgttcc
     5941 tttacgaaac agcattgcac ccattccaaa agcagaagca agttgcccat agccttcttt
     6001 gagtccaata tccacaccaa agtaacgcat gactgttcca ctgcctttga ttaaaacaaa
     6061 cacagcacag atataagcaa ttgtttttat aaaaatattt gttgagatac cttcgccagt
     6121 atttaagtaa tttatgaaca ttgcaaacat agataatcca aacccttgaa atccgactgt
     6181 caaatagcac tgtaaaatat ctgacaaaac ttgttttgat cgttgtcctg tttctaagtc
     6241 agtagcaaat actaaaactc cgagtacacg tttgaatgct agctcgagta ttgctgtaat
     6301 tataatgaaa gcactaaaaa catacgctac cgctaaagca atcaatccaa atgaaattcc
     6361 ccacaggttc gcttgatagc gataatatcc tgatttgata ccatcaacaa aggttgacaa
     6421 aaaattatct gaaaatttag tagcaacaaa ttcattatca gaattttcaa ctaattcata
     6481 acgcaggtta tcagctgttg agttctcttt ttccatttta tcaattacat tatctgtcaa
     6541 tacttgcgta agatccgttt tccaaaaatt ttcttttgtt agattgttcc gatcattcgt
     6601 tttatcgatc atggaatatt gatccgtttt attgatataa gctaaatcag taaccccttg
     6661 ttcaatcagc gaccatgcca caccgccatc ttgattgtta atactagttg cgtcattata
     6721 aagagtcttt gcaaacatta ttcctgaaag tttttctgtt gatccaatca aagtcggcat
     6781 taaaagaatt aatactatag acattacaac attcattcca acagatttta gatcaactgt
     6841 tccttttcca gtcaccattt tataaccaat aatcactaat gagagtacca ttaaaccaat
     6901 aacaactgta tttaaaactg actgatagac tttgttcaat ccagcacttt ccacaaagct
     6961 aaacaaatct aaaacctcgg gaatcatatt ttgaatgcca tcgacaacag cgaacattga
     7021 ttttataaca gaccaaccta tccacatacc gaactggctg taaaaatttc cgttatattt
     7081 tttaagataa ttctgataaa gtggattagt taaaaactca gtttgactct cagtatcatc
     7141 aaaccagttg aaaatactgc cttctgcaac gttactcgta aaaataatca tagctatgag
     7201 aataagaaaa ctaatcaaca accaatttat ttttctatta tcaatctttt ttttaattat
     7261 tttcattgtt acctccctca gcccattttt ttaggctctc agaaagcttc tgagccattt
     7321 ttttattaac gatgatgtga gttacgtaat catcattaac agctcttaaa tctagaacaa
     7381 tagtatttga gcagatactc tcgattaact caagagtatc tgcgttatga tcaagacagt
     7441 aaatcttatc tactagatga ttttcaatca aaataacccc tcccgtagtc aaagttctca
     7501 attttatgaa gatatccctc tacttcattt aaatcaattc tattcttatt gctgactaac
     7561 ccgttgtaaa attctttttg tagattatat ttatctgttt cttgaatctt tttatataaa
     7621 tcaaccaggg agtccctgtc tttaaaaact tgcttgaatt gttcgcttgt atatcggttt
     7681 tcattaattg caacaactgc ttgaatttca ggccattttt ctttcaactt aaaataacgg
     7741 ggaacgctga aatagttctc aaaaaagaag tctcgtgctt catagtattc tctattttgt
     7801 ctttttatat cctcgacaat ttcgtatcgt gtagagatct tcaaagctga actttcgtta
     7861 atttctgttc cttttcgaaa gactttcact tttgaagggc gttcaatttt ataatttatc
     7921 ccctttttac ttgaataact tttgatacga ccatcaaatt gaaccatatc tccttttttt
     7981 aaccacccaa attttagaaa ttgtttagtt aaattcatcc aaatgtggtc tgtgaccaac
     8041 tctaagttat ccccttgaac aatttttaaa ttcgataaca taaaagtagc attttcatac
     8101 tcttgaccaa acttgtattt atccttttta tatccgttaa attcaaaggt accttgaaat
     8161 gtatgccgtt tcttatctcc taattttttc aatttttctc tcaaaataat caactccatc
     8221 atttaatata tttttgtgat ggtagatact ctcgattaac acaagagtat ctgcgttgta
     8281 atcaagacaa taaatttgat atattaaccg tttttcaatc atattattaa tcctttctaa
     8341 cctatttttt tcagtgtctc tagcatatcc agtctactag ctttttcttg cttagctatg
     8401 tcctcccagt ggttcctagc tgtggtgttg ataaacttca tttcatcaat ttcttcaccg
     8461 agattcagtt tttcatttat agaattaaaa agtcttgtta gcattttgtt tgttatatca
     8521 aaatagtctt gacctcgaat ttctatttta tattctcttt caattgaatc tttcatacta
     8581 gcaatcaact taagatactt ttctctttga acttcagtat atctatcacc tagtctggta
     8641 attatttgaa ttcctttctg atttataata ttttcaaaaa aggaattctg atcttgatta
     8701 atctcttcta tactatcctt atcttgatta gtttgatctt gactattctg atcttgactt
     8761 aactgtgtat ccgatttgac gtcgcatggt ataccagctg gataccattt ttccatcaga
     8821 tcaactctta atggttctac ttgaccagac tgcaaaatca tatcaaattt tattggcttt
     8881 agctttgaga aaaattctgg ttttaagttc aataacttga atcgataaat agaaggttta
     8941 tacctatcga ttcgtatatt ttgattttgt aaccagtggg gtagaaacgc taacaaatct
     9001 tcatttaaaa aaaatacaaa accttttgta aaaagtatct ttacatcatc atcgggtgtt
     9061 ccgagcattt tcgtgtatgg ataagcttcg acaattccgt catcatctgc gtttgtgttc
     9121 agatatacgt ataataattt tgctgaatct gacatcttca taaaacttgc attatttaaa
     9181 aatgatttag ccagcattcg tttttctgcc ataaaaatca cacttcttcc tatttgaatg
     9241 ttaaaatgta gtcaaataaa gtttagctag aactttatta ttgagcgttc ctagtggtct
     9301 gcaaactaac gggaacactc ttttttattc ttattaaaac cagcaatact agttcacctt
     9361 ctttcatcct tttacatcca attaaatttg atgctgtagc atccattttt ttacctcttc
     9421 ttttgaatat agttcaattg tgtcaaccac gataataggt aatcctaacc ttttaaactt
     9481 cagaaaagtt ccatatgata cgtttagaaa ttttgccatt tcttttccct taagaaaagg
     9541 tcgttctaat ccgctgtcta ttcttgcttg atcaatagct tcttttgtca cttcaaacac
     9601 atgtgtcttc aactgatttg tttgatcagt attcaacatt acactgactc cgatttgctc
     9661 cattaaaaat tcccactttc atattttcat caataagcca actcgccaag ttgtttgtta
     9721 ataatctgat ttcaataacc aacctattat tttttgataa acatttttcg acacactttg
     9781 aacttcgaag cattcaattt ttttaagagt ttgccttgaa attccaatta attctgctgc
     9841 tttagttttc gtaatcatca actttcctcg tctctctcga attcttatac taaaatcctc
     9901 attaatttga aacataaaca tccctccatt tttatttttg catttatccg tttatccgat
     9961 gtcggataaa tagattttag tatatattat tttgatagtc aattaaaaat atacattttg
    10021 gataaaattg ttcatttttt tatccaaatc atatattata gatagtaaga ggtgaacttc
    10081 attgactcaa aaaaacagaa ttaaagaaac tcgcgaagaa cagaatttaa cacttaaaga
    10141 acttagcaat ctgtcggata ttccgtatca aactttgaga aactatgaga ttggtaaaag
    10201 aaaacccaga aatgatgaag tttggtggaa gttaggtacg attttaaacg tccgtgggcc
    10261 ctacttaaaa ggagaagata gttttaaaac aaatgatgaa ttgcatattt attatctaaa
    10321 aaaaagtcat acaaattttg aactgtctaa gaaagaagaa attatagata taatagataa
    10381 actaaacgaa gacggtttag atgaagcctt acggcagtta gcactattat caaaaataga
    10441 tgatttcaag cggtaactta cttatcatca tgtagactat caggtatcat aaactaattc
    10501 ctcaatcagc caactcgcca agttatcaga ttggagagat tagttatgtc aatacaaaag
    10561 tacaaaacaa aaagcggtac acgatatagt gtccgcttat atgttcgtaa agatgaaaat
    10621 gggaagcaag aatactatgt aaaaaaaggc ttcaaaactg aaaaagaagc caagttacac
    10681 gaagcaagaa aaaaggtcga gattgaagat atgggctatc acagaccttc taatgactta
    10741 tttagaacag tctatgaaga atggttgccg tcttataaaa atcgtgtaca agaaacgact
    10801 tatcaacgaa caaaagacct ttttcgcttg catatcctgc caaagttcgg agacaaatcg
    10861 atttctaaga ttactcctat ttactgtcag aatgcaatca atgaatgggc tgtaaaatat
    10921 acaaatatca agcaacttaa atcctacagc tctcaaatat ttgagtatgc aatcttcgca
    10981 gaattgttac agcgtaaccc tatgacaaat accaaactgc caaaacaaga aaagaaagaa
    11041 tccgaaaatt atttctcact agaagaatta aaagagtttt tggaaattct caataaagaa
    11101 gagtctttaa aacatatcct gatctttcaa cttttaatta ctaccggtgt tcgcaaaggt
    11161 gaattatcag ccctacgctg ggcagatatt aattttgaag aacagttact acacatcgga
    11221 aaaagttatg caacaattcg cactgaagat ccgaatgcta ctagaaaaac aatcaggatt
    11281 cagaagaata caaaaaatgt ctcttccgaa cgaattttac cgattgatac tcaaaccatc
    11341 gaaatgctga aactttggaa aaaagagcaa gcagaggtac ttttacaatt cggcattaat
    11401 gcaaatacca aaaagcaact tatctttacc tatgtaaatg caaatggcga tgttaaccaa
    11461 cctttacacg cagattattc caataatatt atgagacgtt tagagaagaa atataaattt
    11521 aagcacgtta ctattcatgg attgcgacac actcacgcca ctttactttt agaaggcggt
    11581 gcgagcatta aagaagcaca agatcgattg ggtcataaaa atgctgaaac tacgctcaat
    11641 acttattccc atgtaactga aaaagcgcaa cgaaatgcgg tagacaagtt tattgcttac
    11701 actggattat agacaaaaaa gttgccgtca ttaccttttt cattaccaca acgaaaaaaa
    11761 gcaccttcca actgaatgga aagtgcttga aatcgttggt ataacaacga ttaacgctta
    11821 gagaattgtg aagctttacg agctttctta agacctggtt ttttacgttc gaccatacgt
    11881 gcgtcacgag taagtaagcc agcacgttta agagctaaac ggaagtcagg atcaacttct
    11941 agcaatgcac gagcgatacc atgacggata gctcctgatt gtccagcgta tcctccgccg
    12001 tttacgttaa caaatacgtc atatgcgcct tttgtttcag taacagcgaa aggttgattg
    12061 attacttcac gcaagtcagc atgtggaatg tattcttcaa catctttttt atttacagta
    12121 attttgccag ttcctggtac tagacgtacg cgagcaactg cgtttttacg acggcctgtg
    12181 ccgatatatt gaacttgtgc caatgaaatt ccctcctatt agattaagtt tgtgatgtct
    12241 aaaacttctg gttgttgtgc tgcatgtgga tgttctgctc caccgtatac gttcaatttt
    12301 gtaaattgtt tgcggcctaa agtgttttta ggaagcatac cttttacaga agtttcgatc
    12361 aaacgacgag aatttttagc acgcaattcg ccagcagtga ttgatttcaa tcctcctggg
    12421 tagttgctgt gacggtaata aattttgtca gtcgcttttt tacctgttaa tttcactttg
    12481 tctgcattga tgacgatgac gaaatcgcca gtatccaaat gaggtgtgaa agttggttta
    12541 tttttaccac gtagtacaga tgcaacaact gttgaaagac gtcccaatgg aacatcagtt
    12601 gcgtctacta cataccattt acgttctact tcgcctggtt tggccatata tgttgtacgc
    12661 acgttatttt cctccaattt aagttctgat gtttgacata cacttcgagt ttccggggct
    12721 ctttgtgggg caaacaatac catttaacat attacccgct aatctagtca atgtcaacgt
    12781 ttttctgctt gattgaaaaa tttatatgaa actttacttc ttttctttct tttatataaa
    12841 agggtcgttt cctgtcaaaa tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa
    12901 ccccttattt tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    12961 cataaaacat aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag
    13021 gcttacaacc tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    13081 cggcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg
    13141 atttcacaac ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg
    13201 ctttgcccaa atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga
    13261 taaagatatc ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt
    13321 aaagatctgc tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac
    13381 gatcccagcg gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag
    13441 aataaggtga accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca
    13501 aatcatccat gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt
    13561 accaatgtgt ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta
    13621 atacttcttt ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta
    13681 aaataatagt agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc
    13741 agttcgcttt tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag
    13801 ttgagcaaat tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata
    13861 gattttctga tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat
    13921 caattcctgt gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac
    13981 agctcctaat gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc
    14041 agccatcatc tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt
    14101 gagctgattt tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc
    14161 tagaatcgcc ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc
    14221 aaacgcatct gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag
    14281 accattggca cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc
    14341 tcctttttct ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa
    14401 gatgccagca ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata
    14461 acaaaacgtc tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc
    14521 gctcgttcct acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga
    14581 aagcggcccg tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc
    14641 tgctgttttt gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg
    14701 ctctattcga atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc
    14761 aaaatatcca gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata
    14821 aaagatatag tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct
    14881 gttttgcagc cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg
    14941 ccgataaaat tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg
    15001 gttatctgcc caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag
    15061 gccatgcatc gcagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt
    15121 aattacttct tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc
    15181 catcccttgt gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc
    15241 ttcatctgtg aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac
    15301 gttcattttt tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc
    15361 ttggttttga aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg
    15421 atcattttta tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag
    15481 atctcattgt gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc
    15541 gatcatcgtc caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt
    15601 gttggcagaa gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag
    15661 aaatttttcg acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt
    15721 ttacttcatg gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg
    15781 gttgcaggcc ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt
    15841 gtgccttcgc tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg
    15901 gagtggtggc ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg
    15961 aatatccgtt ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt
    16021 cattcctttt ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt
    16081 tgtgacaaac gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga
    16141 cttggagcta ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc
    16201 tcagtattag ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc
    16261 tttttcggct tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga
    16321 accttctcga taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc
    16381 ttctgcagga taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg
    16441 cccgctgtct tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt
    16501 atagtagatc actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt
    16561 cggatcggtt tgctgccaat agatttccac caactgatca taagaaataa cttctggatc
    16621 atattcgatc tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt
    16681 tggatttggt acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc
    16741 ttcaaatggt ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat
    16801 ataaaatccc ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct
    16861 tgtgacagaa aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac
    16921 gaacgtactt gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt
    16981 atgttttcac ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct
    17041 tctgaatctt caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg
    17101 agaaaaactt ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca
    17161 aaataacttg tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc
    17221 aatccgatct tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa
    17281 acggatgttt catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca
    17341 aaggctgatt tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc
    17401 tctagtgact cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga
    17461 atggacagct ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca
    17521 agaacgccat ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat
    17581 gaacgacttg aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt
    17641 gctgtaaagc ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc
    17701 caacatctgc tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa
    17761 cgccctctgg tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat
    17821 ctagacgtcc ttcgcctacg attacgaggt ctgcttgctg tacccgttta tcgaactcca
    17881 gtaaatccaa tacgtagtcg atgccagaga ctattttccc tgaagcaaat gctaacagtc
    17941 ctgcacccat tccaccacca gcactactgc ccgatgcctc gattacttga ggggcaaatc
    18001 tcatataaaa atcatgcatg gcttggtcag ccactgctaa ttcattttct ttcaagcctt
    18061 tttgtggtcc gaaaacaaaa gaagcgcctt cttttccgca taaaagatta gtaacatcag
    18121 taatgacagt gattttggct ttaatcagct caataggcac gttcgtttta tcaactgtag
    18181 caattttacc tagattttct cccagtgctt ctactttttg attatcttga tcgtaaaaag
    18241 cataacctag accttctgcc atgccgatgc cgccatcgtt ggtactactt ccgccaacgc
    18301 cgatgatgat gtgtcgaaca ccttgttgca acaattgtcg tatcagttct cctactcctc
    18361 gcgtggataa cctaagtggc tgttgttggt ttagtgggac atctttcaat ccgacgattt
    18421 ctgccatttc aaaaatcgca gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc
    18481 caaatgcatt agtaacgact gctgtttgcc gcttcaacct taaactgtct gctaatgatg
    18541 aaaccgttcc ttctcctcca tcgcctatag gtagcagatc atattcagcc tgaacaaaaa
    18601 cttcttgaaa acctgattga atggcttgtg ctgcctctaa ggctgtcaga ctttctttga
    18661 aagaatctgg cgcaatcaga atacgcatta ttttcccctc cgttccaaga tcagttcttt
    18721 ttccagctgg tattggtctc cattgacaaa aataggtaat gtttctttcg tctctttttt
    18781 tattactatt tgtttttttg tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg
    18841 aaaagaaatt ttttcccatg ctgaaggaag tcttggagaa atagaaagag ttccattcgt
    18901 tactgtaata cctgaaaaac caaatataac tgctagccat agtgctccta atgaagcagc
    18961 atgaattccg tcatctgatg aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg
    19021 gaataaatga tacgcccatt ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc
    19081 tttactcaac gatgagtcgt ggattgtttt gttttcatag taatgcaaat tcttagaaac
    19141 gattggttga gaaaaatact caggtaataa gtataacagc atgacaagat ctgcttgctt
    19201 gctgacttgg agttcattta cttcgctacg ggcataatca agtaaaatag actggctgcc
    19261 aggacgttct ttgtaagtgg tcaaatcaat aactggctta ctgagatacg tgtcatcttg
    19321 cggaatcagc tgttctttgt taggcactgg caaatataac ttatctaaaa aattttgaca
    19381 ttctttcaaa aaaagcggat cgccagcttg atatttaaca gctaaggcca cattataatg
    19441 cgccagatag ttcgtatatg cattattgtc gatatattct gtgtattcat ctggaccaat
    19501 gactctgtgt atctctaaat gatcatcgac ttttacagct ctgctcagcc aaaaagctgc
    19561 tgtttcatga atgagcgttt ttccctttgt ttctaaaaac gattgatcct ctgttgcttg
    19621 gacatactct tgaacggcat aagcaaggtc tgcactgata tgatgttctg cggcagctga
    19681 agcaacttct tgtcttgttc cggttttgat attcaatgca gcaaatttgg gtgtctcttc
    19741 ctgaccagtg tgtgcgcttt cccatgggaa aagtgcacct tgatagtgat aatctcttgc
    19801 tttttgccgt gcgccttcta atcgatcata acggtacagc agcaaattct ttgctacttc
    19861 aggaaactga tataaaaaga aaggcaggat aaaaatttcc gtatcccaga aaacatgccc
    19921 tttataacct tcgccagtta gtcccttcgc ccctacacta acacttggat cattgcgggg
    19981 agtcataatc tgtaaatggt aagcagcgaa gtccatcgct aactgatcga aagaagaatc
    20041 gccttggatc gttatccgat gctgatgcca aaaatctgtc caataagaca tcgactgctc
    20101 taaaatttct gtataactaa cgcttttctg ggcttgggca atacttttct taaacgggtc
    20161 tgtttgctga agagaggtag agagcacgat gatcttctcc agcgtaaagg acgtattttt
    20221 tctgatcttt tctgaaaagg aagcggtgat ccggcgattt tttgcttgga atacacctgc
    20281 tttattgaat ttagctgcaa tcccaatatc aattttactt tctgttgtct ggtaggatgc
    20341 actgatcaaa tcgtcaccaa atactcgcag ttctttttca accaactgct gtgtaccaaa
    20401 attggtttgc tgtccatcga tacccgtttt cacatctact tggcagtctt cggtcaacgg
    20461 cttgatgacc actttcaggg ctgcctgatg gagattattt tgatcagcaa cacgacaagt
    20521 aatgacttcc actttttttc cttgagcagt ttcaatgagc gcatagcgtt taagctctcc
    20581 catttctaaa tctagtgccc gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg
    20641 ttcaccgtcg atttttattt cccatctgat gacatctggg agattcacta actcggctga
    20701 ttctgctcct aaaggtcggt tatatacacc agctgcaaac attcccctta cttgattccg
    20761 atagtcttcc tcctggcttc ctctgatacc aaaaaatcca tttccaatgg tcatctgact
    20821 cgcatactta ttgatatact ctaaatcaaa ctgtttttct tctactttca taaggtaata
    20881 acttctcctg tttctgccga acgatataaa ttttccacca gattctggat gacatatcct
    20941 tgccagccat ctgcaatcat ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc
    21001 atacttagtt catgtgggtc attaactggc gtggtctttt gtaaggaaac cagctttcca
    21061 tctacatctt cgaatagttc gcctggatac agattagcac ctgcctgatc gccacaaaaa
    21121 gccacattca gtttgtcttc tctgatattc aaagcaaacg aagtattcag ttggatgatc
    21181 ttgccgtttt ctaattccag tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga
    21241 tcccatttcc cgaatttccc tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc
    21301 cgaacgcttt ttactttagg aaatccagta atatacatcg ctgtatcaag catgtgaatg
    21361 ccataatcga tcaaaggccc gcctccttga agttctttgt cgataaacgt tccccagcct
    21421 ggaacaccag accgacgaag cgcattcgct tctacgtaat aaatatcccc gagaacatca
    21481 agatgattct tgatcattgt tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa
    21541 atctgcccgt ttttatcggc tgtctctgcc atctttcttg cttgatcagc tgtcatagct
    21601 ggtggtttct cgcacatcac tgaagcatta gcttcgaggg cagccaatac atgttcgaag
    21661 tgaaagcgat tgggtgtaca gacactcacg atatcgggag aaatctcatt cagcatttgt
    21721 gtaactgatg tatacgcatg agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg
    21781 ctgactggat caaccacagc ggccaattct aagacatctg atcttttttg ataataagca
    21841 gcatgtactt tttctgctac ttgtccagcg cctatgattg ctactttctt catttatttc
    21901 tccctccttt gcttatttac ttattgttat tctttctcta ttctttctct aaatcattta
    21961 aatgacgcgc tgtttccaga cagtttttca agtattgcaa tgattcctga taaattttca
    22021 cttcattttc tcctcgcaaa cgacattcat ataccatata accttcatac tcattttgaa
    22081 gtagcgtttg aaaatggcga gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat
    22141 tatctgctaa atggatatga cccacatact ctttttgcgt agtcaatgct tttgaaatat
    22201 catcttcttc aatattcata tgataaaaat cagcgatgat ctgcacatgt ttcaattgat
    22261 tagagtcaat gtaatgacgc gcatccgcta acaaattgat catatggtct tggtatcggt
    22321 tcaaaggctc caaataaata accgtacctg tttcttcggc tactttatct aaataacgaa
    22381 gtgattcgct gactgcttta tagtctcctt ctttgcttcg gggtgaaacc atcggcggta
    22441 atcgataagt gaacattccc caagctgctg ggataacgat tcctttgcct ccaacctcgc
    22501 ttagtgcacg caaaatctca gcaatttgac gtacaccatt aagacgccgt tcttcgataa
    22561 agtcaccgat ccacccatcg tagccgttac aagctgtcac tactgggatc cctgtttctt
    22621 tgattgcctg tttgactgtg tcgatatttt ctattaacaa cttaccgtcg atttcgtatt
    22681 cttcaaatcc acagtctttg ataaatctaa atttttctaa aatgtctctt gggaaaaatg
    22741 cttgatcttg cgtaccgaat ttcattgttc tcctcctcgg acttgaacgc ccattttgat
    22801 actttcttct gggtgctggt ctacataacg catgtaagct tctgcgcttt ctgcaaatgg
    22861 cacaactgga tcaatgattt cttcacaatc caagtaaccg ttcatcaata attcccaaca
    22921 tgtgtcctcg attcgtttac ggttccatct tgggtaatct gggtttggtt cactagatgc
    22981 tcgagcaaag acgatttttg cattattgaa atgtgcttcg cgaccaagat taaacccagc
    23041 tggaaaaggt ttagcaaagg caacataaga aatgatcccg ccatagccga tccctcttaa
    23101 agaagcttgt aatgcggcag cactgccact cgtttcaata actgaatcta ctccacgttt
    23161 atctgtcaat tcctttagac gaaaaccgac atctgctttc ataggatcta gcgtgtaatc
    23221 ggcaccgtat ttttctgcta attctctacg ctgcgctatg ggatcgatcc caacgatggt
    23281 agtagcacct gcttttttag ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa
    23341 gattgctaca gcatcacctg ctcgaacatg tgcttcccgg ataccaccta aagcgaattg
    23401 agcaggatcg taacatactg catttttcca ttgatccaac cgatcaagtt tacgtagttt
    23461 ataattatca acaccgtttg taatcagcgt ttcacgaatg ggtccataag tgcagactaa
    23521 ctctcctttt tgatactctg tcaccaaact gcccgtttct tcgatcgtac cgacaatcat
    23581 atttcctaat tgaaattcgc caaataccac accgcgttgg cttccttctt ctcttggcaa
    23641 gaataggcgc cattcttctg aaaattcttc atcgataaat ggacttaatc ctctaaagtc
    23701 gacaatttct gttcctggtt ttggagtagc gaaaattact cggatacgta cttcgttctt
    23761 ttcgatctca cggtcttcgt aatctttgaa tccggctatt cttggttcta atgcaactaa
    23821 ttttttcatc ttttctttcc tctttctttt ctttttatcc ttttacgccg ccttcgacta
    23881 aatcaccttt aataaatcgt tcagataacg catacataat gatgactggt aaagcggtca
    23941 ccaatgaagc ggccatcatt cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca
    24001 aaccgattgg taaagtaaat ttttcggagg tcgacaggaa aatcgaagca aataaataat
    24061 cattccacga aaccataaag caataaacga agacagaaat gattccagaa atagccaatg
    24121 gtataatgat ccgaataatg atctggatcc ggctaagacc atcgatcatt gctgcttctt
    24181 caatatcagt cggaatcgtg tcaaaataac tttttagcat gaatatcgca gtaggaagtg
    24241 tttgaacgac catcgtgata atcagtgctg tattggtatc atacaaacct agaccggaaa
    24301 tgattttaaa taatggaaca acgatcaaaa taccggaaaa catataaacg gtgtaaaagc
    24361 tagcattgat tttcgagcga gctttgaagc gtagttttga caaagcgtat gccccgctga
    24421 tcccaatcac tactgcagtt acggaggcta tcaaggagat cactaaacta tttttgaaat
    24481 acatgatgaa tggaaaaatc tcagcattga aaatatcctg ataatgttca aaagtccatt
    24541 ctttcggaaa aatagtttgt gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca
    24601 tcagcatgat caaaaatgga aacaaattga cagctaatag caacaaaatc gtcgcgtaaa
    24661 ataagatttt cctgctcttt ttatttttca tttcaagtcc accttctttc gtgagagcaa
    24721 gacgaccgct gcaataatga tgaacaatag aatcgatata gctgctgctt ttccaagatc
    24781 attgaacgca aaggctgtct gatacaaata aacgcctaag atattgactt tattcgtcag
    24841 tagatagaca tctgtaaaca tatagaacat ccagatagat cgtaaaacaa tcacagttgc
    24901 tagtactggt ttgattgccg gcagtgtcac gatcttgaac ttctgccaag catttgcacc
    24961 gtccatttca gctgcttcat aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga
    25021 aataaacgca tacggatagt atctccagat agcaaacaaa acgaccataa agaagctagc
    25081 aactggctgg tcgaaccaca gcggtgcttc gttgaaaagc tgcaacctat ctactaacag
    25141 atcattgacg attccataac tattattgaa catatatttc caagcaaaaa tcaaagaaat
    25201 agatggcgta acataactta ataaaatcag tgaacgcacc gttttcctaa atctgaactt
    25261 gcggttaagc aaaatagcta cggctaatcc taaagctgta ctgcctgcta caactaagaa
    25321 tgtgtaagca actgttaaac ctaaagaacg ataaaattca ctatcactta aaatttcaag
    25381 ataattgctt aatccgataa aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa
    25441 actgatctga atattggaaa gcattggaat cagcactaaa acagctaata aaagcatgct
    25501 tggtgctaaa agtcccatgg ctaattttaa gtcagaatgt ttccttggac gattcattga
    25561 aacctccccc tgataatctg ccaatagcta tctacttttt tcgaacacca ttggcagaac
    25621 ttcttgttat ttcgtatttt tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc
    25681 gacagttact tggttcactg cttttccgat cacacctgag cttgtgatat ctcccatttt
    25741 cgtgaaattc ttttccccga ctagtccaaa gacttgtact tcatcaaaag cactagcaat
    25801 ctcagatggc agctcgccaa aagacttgat gacttcattt tctttgtaac catccagttc
    25861 gactacttgt ttattcactg gttgagcacc accaggagac atcaagaccc acttttccat
    25921 gtttttcggt tgagataaga aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc
    25981 tagaccagaa gaaatcgtca atccagatac tgtgccatag acagcttgat ctttttgtgt
    26041 tgggatagca aaaccgatat tttctgagtt ccccgcttca taaacagaag gcaaaatgta
    26101 agttgaatag attgccattg gtgctgttcc attcatgaaa gcatctttga tttctgtcac
    26161 atcattagag cctggcatcg tgtattggga taagttttga taaaacgaca atgcttctct
    26221 catcttttcc gtatcgatcg ttacctcgcc ttcatcatcc aaaacatttg ctccatttga
    26281 caaagcaaat tgagagaacg cttgctcact cattgttcct tcagcagtag gcatagcaat
    26341 tccatattgt ttattctctt tatctgtgaa atgttgagca atttttaaaa tatcatccca
    26401 attttctggt tctgaaaaac cagcttctga taatttttct ttgtcatacc aaataccttg
    26461 aacccagccg ctaattggtg cagcaatata gctttttcca tcttctgaac gaacaaggtt
    26521 tttggctccg tcataataat tatcttctcc agcttcctcg ataacgtttt ggactgccgt
    26581 ctgatcaatc agttcatctt tatccatcac tttagcaaaa tcctgactga cttccattac
    26641 ttctggtaat ttaccggaac gagctaacgt aacgattttt gtattgaacg ctgattcttc
    26701 taccggcact tgcttgattt tgatgtctgg attttctttt tcaaaatcag caaccagttc
    26761 attgatcaca cttaatcgtt cctgttcgac agaagagtgc atgaattgaa tcgttgtctg
    26821 ttgtgattcg ttagagcttt gactttcacc acaagcagta agaagaaatc cagtagctag
    26881 cagacaggta gtaacaataa tttttttcat ttttttccac ctcttatttt attattttca
    26941 gccacatata ttcatacggc tggaatatca actttcgttc ttgactgcaa tggataattt
    27001 gtccagaaaa caggtctagg taatctcctt ttggtaaaag ataacctgct gattgattgg
    27061 aaaaattatg gataactaag actttctctt ccttcgcttc gcgaataaaa ctgataaaag
    27121 aatctgctga gtccagtaag taaatcaaac tattcggatg gaaggctttt tctttttttc
    27181 gaatagatag catctgggtc aattcaccat atactgctgt tcttaatgga tctgtctgca
    27241 actctttctc aatatctgct aacaaaaatt tttgccgatt gatttttcga ttctctcctg
    27301 ttttttctac gcctttgtaa tcatttcgac ttcccagcac actttgaata taaattgccg
    27361 gcacgcctgg taacgttacc aaaaatgaat gagcagctaa aaagcgtttg cttctccatt
    27421 ccgtcgtatc tgttggagaa cttaacgcat ctagatacgt agcgtttatc tcatatggac
    27481 tggtcgttcc atctgggttt ttcttgtagt ttactcgggc accctctttc ttcagctgtt
    27541 ccactaattc aaggatcgtt ttttccggca gaatacctcg taccggattc aatccgatac
    27601 cgtcatggga ggctaaaaaa ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt
    27661 ttaaccattc tttcaactta cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg
    27721 gtagcgaaaa ctgatagacc atctgtgctt cattctcgcc attcccaaaa taactgatat
    27781 tatctttgtg tggcacattt gtctcagtga tcaataccgt tcctggagct gcttcatcag
    27841 cgattgcacg gaataattgg atgattttat gcgtttctgg caggtgtata caagaagttc
    27901 ctagttcttt ccataaaaat cccacagcgt ccaagcgaat aaatgaagcg cctttttcta
    27961 agtaaaacaa aagaatatcg atcattttca ataaaacttc tggattttta aaattcaagt
    28021 ctacttgatc ttcgctaaaa gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga
    28081 acgttgtaag taacgggctg gtccttggtc tagtcacgct gcttgtatct gtatccagtg
    28141 gcaaatcaat aaaaaaatct ttgtacgtat cattcaagtt taaatattcc tgaaaccaag
    28201 tactttcggc agacatatga ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca
    28261 tttcttctat atcttcccat gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg
    28321 agaaaccatc atcagatgta tatgggaaaa atggcaagaa atgtacaact ctaaatgtat
    28381 ctgccaaata cttttgataa aatttcttaa atgtacgcaa agttggttgc tgctcttcaa
    28441 aaaattgatc cccataagta atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg
    28501 cctggccttt cttctgctga taaacactga ctttttgttc aatcatagag gcggttttct
    28561 cagacaaatc tgagtcgcga taaatcgctt gtaaaagttt tgtatacatc ggattcccct
    28621 ttcttttttg ggagcgttcc caacgatttg atcatattat ggaaaacgct tactgtcaat
    28681 agcatttttt taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca
    28741 ataattaata gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat
    28801 tttcaaaatc atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc
    28861 ccaaaaagga ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta
    28921 atgtctcaaa atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag
    28981 caaaagcaag tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa
    29041 gaggactgtc aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata
    29101 cgacctatgg caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac
    29161 aaaataactt cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat
    29221 gcgcgaaaaa gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg
    29281 atgaaacact tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag
    29341 tcggaaacga ctacacgaac atctactcag tggacacgga taatttccag gatagttacg
    29401 atttggtgga aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga
    29461 cagaagttta tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc
    29521 acctagatat ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg
    29581 cagcaatgaa tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg
    29641 cattaaaatc attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg
    29701 atctttcgat cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga
    29761 ctcggataga cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac
    29821 taatccacca ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca
    29881 cagattcgat ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag
    29941 gataagtcag ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta
    30001 ttttagcgaa tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt
    30061 tgtgttaaac tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa
    30121 gtttttatag ttcgtttttt taaacacatt tacccttatt tcaatccatg ttatttgggg
    30181 atacttatct ctaaaacata aatagaataa cagacatgtc tactggtttt gttttattcg
    30241 aggaattatt caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa
    30301 tttattttgc attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag
    30361 atgatcgttc cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt
    30421 ttcttctagt tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg
    30481 tcacaacctc ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc
    30541 atttgcgctt caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt
    30601 ggttccatgt cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata
    30661 acattttctt tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag
    30721 acacgagttg tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct
    30781 tttgtgatac ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta
    30841 ttgtagtttt gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta
    30901 tcacctggat ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc
    30961 ttgtcgccga tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag
    31021 ttttcaacag ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct
    31081 gtcaatggtg caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa
    31141 ggaacagtat acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt
    31201 gcaggatctt ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct
    31261 ttcggattac gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct
    31321 ccggcaaccc cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt
    31381 gattgagcgg cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg
    31441 ttgcgtccgt aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg
    31501 attgtcactg tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat
    31561 tggtttcctg caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa
    31621 cgtcccacta aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg
    31681 atttcaagac ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg
    31741 tcaaccagac gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc
    31801 cctttacgag ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag
    31861 ttcgaaatga ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt
    31921 ccggcaagct gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag
    31981 attggatttt tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca
    32041 ttccaaacag caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat
    32101 tgttttgtaa tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc
    32161 agtacactga tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct
    32221 aagtctttca tacggtcaag catcttagaa gtttctttta ctttgaatcg tttgaagact
    32281 tctgcgatga tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg
    32341 atatgtgcag gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc
    32401 aagttgaagt ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag
    32461 atgatcttac caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt
    32521 tcacctaatg agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc
    32581 catgcgataa cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct
    32641 tcttccattg tcaagtagta gttacctaga accatatctt gagaaggagt aacaactggt
    32701 ttaccatctt tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct
    32761 tgtgcttctt cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta
    32821 taagcttcac atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg
    32881 aatgcttgga taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc
    32941 ttgattacgt cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc
    33001 gcatttttga tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg
    33061 aataattcaa tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca
    33121 acaacgataa cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa
    33181 cgaccttgtt tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca
    33241 gtaactggac gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata
    33301 cgtttttcat tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg
    33361 ttattacggt tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca
    33421 ccttctaatt gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc
    33481 atccagcttg gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt
    33541 gcacgtgtac gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct
    33601 gcatctagat caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg
    33661 aattcttgtc catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt
    33721 ttctctaaat tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct
    33781 tccaaagcgc gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag
    33841 taccagatat gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact
    33901 ttcgaacgag taacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt
    33961 ttgtattttc cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac
    34021 aacccttcgc gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca
    34081 ccgtaagacc agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat
    34141 ttatttacat cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg
    34201 ctgatcttcg accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt
    34261 tcaaagtttt ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct
    34321 aattctttgg cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg
    34381 tcgtcttcat cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc
    34441 atgtccagac ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct
    34501 ggttttggaa ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg
    34561 tcatctgatt tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt
    34621 gcccatactt ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt
    34681 tgttgtgtaa ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg
    34741 tgagcaagtt tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct
    34801 gtacgtccgt cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt
    34861 tcccatacgt cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac
    34921 tgacgtgcag ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc
    34981 acccctaaag gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct
    35041 tcttccggca taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc
    35101 ccttcatgga ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt
    35161 gacaattcat ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg
    35221 ccatgtggta cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca
    35281 tgcaatagac gttcttctgc agataattct gttacccctt ttggcgtgac tttccctaca
    35341 agcaaatcgc catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct
    35401 tttaatgcgt cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt
    35461 gtatcgcggg cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt
    35521 accaaacgac ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa
    35581 ccaactaaaa cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc
    35641 agagtatccc ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt
    35701 gttccggagt ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg
    35761 cggcgtacgc ggatttcaga agcatcaacg tattcaacga caccatcatg tttacataac
    35821 aaagcagctc ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct
    35881 tgaggattga tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg
    35941 gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt
    36001 gatacgtcca tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga
    36061 gccataacaa cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct
    36121 acgatgtaat ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct
    36181 gtttcacggt ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca
    36241 taactggaca aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac
    36301 atacggccat agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc
    36361 aatccgccag gtcctaaggc gaaaagacga cgtttgtgtg tcaattcgcc aagagggttt
    36421 gtttgatcca tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca
    36481 actggacgga tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt
    36541 ctttcacgaa ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt
    36601 tcaccgactg aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata
    36661 ccttccatca agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt
    36721 tttacagctg cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt
    36781 ggtgaaaata ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat
    36841 ggatagtaag tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact
    36901 tggtgagtca gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat
    36961 gtttcagcta atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg
    37021 taacgaccaa cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag
    37081 ttacgtgagc tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc
    37141 aaaccttctt ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg
    37201 cgtaatgttt cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac
    37261 gcacgaacta atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg
    37321 tcttttgcat cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg
    37381 aatccttctt ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact
    37441 aactgagaaa cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt
    37501 aatgggaaat caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc
    37561 aaacgcaatg ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct
    37621 actgtatact ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg
    37681 aagtcatcga ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat
    37741 tggtaagagt ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt
    37801 gcgaaacttc tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc
    37861 acccctctaa aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa
    37921 tattattttt aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa
    37981 tagaacattc tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag
    38041 ccgaaacgga caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat
    38101 atactacact tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca
    38161 acattttctt aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag
    38221 acctttttat ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac
    38281 caacatcaac caaatcgcac caacacaact gaataacttt tactttagct actttagata
    38341 ggtttgtatt catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta
    38401 aagaactttg tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga
    38461 aaatacaaag taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat
    38521 ccatttggac attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa
    38581 atgaaaaaaa atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc
    38641 gcacaaaaag acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa
    38701 cttgtacaac ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa
    38761 gaagggtata aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa
    38821 gacaacctga aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga
    38881 atcgccactc cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc
    38941 acagcggtca cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc
    39001 aatgtgactg gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat
    39061 atcgtgccag acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag
    39121 atccaagctg atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg
    39181 acagcaaatt ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat
    39241 ggaatctata ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc
    39301 gcaaaagaaa caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt
    39361 ctagcaacag taggaatcga ctacgaaagc ttgggcaaac aaacagggat catggcagca
    39421 aaaatcttag acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa
    39481 ctgtatgtca ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa
    39541 gctgaataaa acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg
    39601 catcgcaagg ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct
    39661 tggacattgc cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag
    39721 taagtattac aaatggctat catccattga cagcttgtct tctaggcttt ttagggagtg
    39781 cggcagcagg gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg
    39841 cagggatcat caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga
    39901 acatctcttt attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa
    39961 aagacaacgc tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg
    40021 ttttgttctt acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa
    40081 tgagcgaagc aaacgggatc aacacagata atatgaagat catcggttat atgatttcta
    40141 atggatgcat tgctctttct ggctccttgc ttgcgcaaaa caacggcttt gctgatttaa
    40201 attctggtat tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt
    40261 tacgtaacca gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc
    40321 gttttatttt agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt
    40381 ctgctttcgt gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac
    40441 ctcaaaaaaa gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca
    40501 tcataccttt gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc
    40561 cttagatccc ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt
    40621 gctgaacagc atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa
    40681 aaatatcacg aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga
    40741 tcctaaacta gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa
    40801 acgaggaaaa aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa
    40861 agaccagctg gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt
    40921 gctgtctgga ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga
    40981 actgatctta ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat
    41041 ggaattgaca gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga
    41101 catggaagac gcgatccgct atgggagtcg actgatcatg ctgcatcaag gaaggatcgt
    41161 cgtagatatc cagcaagagg aaaaacagca gctcacagtg ccccaattat tagcgttgtt
    41221 caaacaaaac agcggcacgg cattaaaaga cgatcaagta cttttatctt cataatcaga
    41281 aagaggatgg gacaaaaaat gaacgcccac cctcttcttt tttctctttc cttctttcgt
    41341 ttttgcttta tgtatcaacc gttgtttctt cgaaaactat tttgttctct ccttcttcct
    41401 gatagaatgg atgggaaagg agcttgatgc catgacaacg aaagacaagg tacttcaatt
    41461 attgaaaaag acaccagatt ttctttccgg agaaaagttg gcacagcaac tggaagtttc
    41521 ccggacaagt atttggaaag ccatcaaaga attggaaaag gcaggctatc gttttgagca
    41581 cgggcctaca gggtatcgtt acctgccctc tgacgtacta gatgcaaaag aaatccagca
    41641 agagctacgg tcgtttattc ctgatttgaa agtacaaacg atgttgactt ctgaatctac
    41701 gatgaaagat gccaaactgg cagcagctca aggagattct gtttcgaagt tatttgttgc
    41761 tgatacacaa gaacaagctc gtggacgttt tggacggcct tttttcgctg aacctggacg
    41821 aggaatctac atgagtctct tgctccatcc agacaaacgg tttgatgaat tgcctcaata
    41881 tacgatcatc gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc
    41941 ttcgatcaaa tgggtcaatg atatctacat ggatggaaaa aaagtctgtg ggattttatc
    42001 tgaagcagtc agtgatatgg aaagcggcag gatcagtcat atcatcattg ggatcgggct
    42061 taatttttct gttcctcaaa atcattttcc agctgatctc caagaaaaag ccatctctgt
    42121 ctttcccgac ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga
    42181 attgttcact tatctttctc acccagaaaa agcgttggac ttctataaag aaaaatcatt
    42241 cgtattggga aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa
    42301 agaaatcggt gatcatggcg aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc
    42361 ttctggcgaa atcagtctca catctatccg tacgcaataa ttatcagatt tcacaaaagg
    42421 acacctgatc tcatacagtg tccttttttc tgaattctct tcaaaagact ctccttattc
    42481 tatcagctga cagtttctct atcctctaga aaaaggtatt ctaaaaatat ctataaagca
    42541 tggtacactt tctttaaatg aatcaatgca aaaaacaaga gagtcatcag tttacacgat
    42601 caaaagagat agcaggccgc ttcctttttc aatcttatag atagaccatg ggtaacgata
    42661 aaaacaaaaa agaacttgcg acttattttg ccgtaagttc ttttttagct agtgccttaa
    42721 tctcttaatc ccaacaaacg tctcgcatag atcacctgac cagagtgcat agcagcatca
    42781 tcaataatag aaacgagacg cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt
    42841 acttcatcta gctcggaagg ttccagcctt cttaagtaag aaatagcaaa atcagcagct
    42901 cctcgcaagt atcctagcag atcttctatt tgattagctt ggatctgctt tgcttgttcc
    42961 agactatgct tccaatcttg ttcgttatct gctatcgtca tcggaaaccg gctttcccag
    43021 tcttgactat gccaaattgg agaagttcct gctaaatcag caatctggaa atctaattct
    43081 cgagcggtat gccaagccag ccaagtcatt gatttgatct gcggggctct atcttccact
    43141 ggaaaacgat tggcctcctc aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat
    43201 gcccgatcca cactctcgca tagcatatct aaataatcca tttattttcc tcctttttat
    43261 ttatcctact tactttttcc taattctagg atacctactt atatgttact acacaccctt
    43321 attttttaca ctttaacagt caccatcatc aatagtttgg agattgaaag aaaaagatag
    43381 tgtcgaacta tcaattgaac aagaagcaac aatatgaacg tttcattaat attctttaaa
    43441 taaacaatga tttttcaaac gaacagttta agactaggag aaaaagagta taataggaaa
    43501 atgtttgaaa ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact agtcctagtt
    43561 gctttgttgt tcaatatctt ttcgtatggt gtggccgttt cagcggaaac atacattcct
    43621 aaagaccctg ggaatattta taatgatcta actggtaact atggcttgtt aggaattgct
    43681 agtcaattcc acgtatttgc taaaaataag acaactgtga atgcacatac agacggaaat
    43741 atagcaacac atgaacttga tgcaaaaaat aattttggta cagagcttca tgaaggttta
    43801 ttgaaccaag aaataaatta tatacaaaaa actgattctt tcgctaattc ttcaggcata
    43861 cctacaggta gcacaagaat gaataaattt gtcgttggca gtaacgtgga tgtagcactt
    43921 caagatggtc atcctacagt caatggatcc aggcttgatc acttaactcc agatgatttt
    43981 tataaagatc gtgctgggca tgaatatatt gatttcgagc aggaatttgc taaattgaat
    44041 gtagcagcaa atgatcttgc aacgatcact ccagctaaaa catatactgc tgcagatttt
    44101 ccagacatga acaatcggac aatcgattta actgatttat cagataccgg gtttttattg
    44161 gtaaatatcg atgcagaagt gttaacgatg aatacgccac ttcaaatcat taatccaaat
    44221 gatcaagtag ttgtatttaa tgtcatcaac agttcttctg cattgaacgt acagtcacca
    44281 atcaaataca atgatcgttc aaatcatgaa acagaagatt tttcggatgc gaatatctct
    44341 tggaattttg gaaatgagat gactgatttg acaatcagtg ctccttttca aggaacaatc
    44401 cttgcgccta atgctactat ccgcattact caaaaccaag acggaacgat tataggaaca
    44461 aatgtgattt tagatgcagc taccaatcgt tgggatccta acgaaatatt tatcagcgac
    44521 aatggtacag atactacaga cacaagcgac agtacagata catctaccac aagtgatagc
    44581 tcagattcat caacttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    44641 gatacatcag cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggac
    44701 acatcagctt caagtgacag tacagatact acctctacca caagtgacag cacggacacg
    44761 tcagcttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca
    44821 gcttcaagtg acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct
    44881 tcaagcgaca gtacggatac atctaccaca acaagtgata gcacggacac gtcagcttca
    44941 agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc taccactaca
    45001 agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc taccacaagt
    45061 gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac cacaagcgat
    45121 acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac gaactttaac
    45181 ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact tcctaaaact
    45241 ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt aatcggttta
    45301 agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca agataaaaaa
    45361 gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa atacaagaac
    45421 tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat ttacgtatcg
    45481 aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa aagagattct
    45541 cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt
    45601 aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg attttgcgta
    45661 cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt agtagctttg
    45721 gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga cgcatgtctt
    45781 ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata tcatcgataa
    45841 tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg aagcgctcgc
    45901 ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca gctgtcttac
    45961 ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga tcatccatct
    46021 gacctaattc gccattcaat actttttcca tactgcgaga attcaattgg agacttttca
    46081 aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag tctcctgcat
    46141 agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt cgcagatccg
    46201 cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg atagaagctg
    46261 ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac aatagctgat
    46321 aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgattgct tgttcgacag
    46381 gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc aattctttac
    46441 ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc cttgtgtgac
    46501 gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc ataatctttt
    46561 tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat cccgattgca
    46621 atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac tgtccatgat
    46681 tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc gccggccgca
    46741 ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa cattccaaaa
    46801 taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc gcctagcaaa
    46861 gtagtcagga atagtcgtag ccaaagtagt aagaagctgt ctcgtttttt catggtaaaa
    46921 atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc aaaagaaaaa
    46981 ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac aagtagcgaa
    47041 atataaatca ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa tcagactgtc
    47101 ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca aacagacgat
    47161 ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat cgcaatctgc
    47221 ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc cgtgttcatc
    47281 tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt tgatttctgt
    47341 gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct gaagaccaaa
    47401 aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa catcccaagg
    47461 tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc tttttccaga
    47521 ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag
    47581 taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg ggcttattac
    47641 cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag tgggcaactt
    47701 caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa tttcggctta
    47761 tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa cagaggttgt
    47821 gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt aattttacat
    47881 aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata
    47941 acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt
    48001 acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga
    48061 accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc
    48121 tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa
    48181 tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa
    48241 cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga
    48301 cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc
    48361 ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc
    48421 agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac
    48481 actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc
    48541 attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag
    48601 tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc
    48661 atctttgata tggaaaaact catgcattaa atattggaac atataataac cagaacgaat
    48721 atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa
    48781 atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc
    48841 ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc
    48901 tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg
    48961 gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt
    49021 tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc
    49081 agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt
    49141 gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa
    49201 taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa
    49261 catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa
    49321 tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc
    49381 ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg
    49441 ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc
    49501 aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc
    49561 tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat
    49621 ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg
    49681 agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac
    49741 ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac
    49801 atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc
    49861 tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag actatctata
    49921 taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc atatattata
    49981 tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc
    50041 gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa
    50101 aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg
    50161 tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt
    50221 tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag
    50281 ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga
    50341 atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga
    50401 aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat
    50461 cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga
    50521 atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt
    50581 gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac
    50641 tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg
    50701 taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct
    50761 tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg
    50821 caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga
    50881 tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc
    50941 tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt
    51001 agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt
    51061 agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga
    51121 aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc
    51181 agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc
    51241 ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa
    51301 acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag
    51361 ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg
    51421 aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt
    51481 tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac
    51541 gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat
    51601 atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct
    51661 tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat
    51721 gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc
    51781 gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact
    51841 gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac
    51901 caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct
    51961 attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta
    52021 gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat
    52081 atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca
    52141 gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg
    52201 acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac
    52261 accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa
    52321 ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg
    52381 gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata
    52441 cattggctaa acactttttt attttttatt ggaatgctag tttttgattt agcgactacc
    52501 gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa
    52561 aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg
    52621 attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt
    52681 ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc gattccttta
    52741 tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc
    52801 atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca
    52861 tctggacaat ttgctttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc
    52921 ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac
    52981 cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca
    53041 gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc
    53101 ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat
    53161 ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat
    53221 atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc
    53281 atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca
    53341 aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    53401 ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
    53461 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc
    53521 ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa
    53581 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg aaggacagct
    53641 gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc
    53701 cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta
    53761 actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca
    53821 cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat
    53881 attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat
    53941 aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca
    54001 taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg
    54061 tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc
    54121 ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat
    54181 cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa
    54241 gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata
    54301 atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc
    54361 gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt
    54421 aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa
    54481 acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat
    54541 cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat
    54601 aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg
    54661 tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag
    54721 caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt
    54781 tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct
    54841 aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac aaaatacacc
    54901 tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt
    54961 taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact
    55021 tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc
    55081 gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc
    55141 taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag
    55201 accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc
    55261 aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt
    55321 gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc
    55381 tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat
    55441 ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt
    55501 tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag
    55561 ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct
    55621 gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt
    55681 cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt
    55741 cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct
    55801 catcataaga taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga
    55861 ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg
    55921 ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca
    55981 tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt
    56041 tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt
    56101 cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag
    56161 ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag
    56221 attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc
    56281 gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat
    56341 ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca
    56401 acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata
    56461 aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa
    56521 caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt
    56581 catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg
    56641 caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac
    56701 catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact
    56761 tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca
    56821 tttctttcat catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc
    56881 taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc
    56941 ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt
    57001 tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg
    57061 ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc
    57121 atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag
    57181 acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag
    57241 tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc
    57301 gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc
    57361 aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc
    57421 atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt
    57481 atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac
    57541 atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata
    57601 cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg
    57661 caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc
    57721 tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa
    57781 cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat
    57841 ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa
    57901 aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa
    57961 ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa
    58021 cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt
    58081 ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga
    58141 ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg
    58201 gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga
    58261 tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga
    58321 ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca
    58381 tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa
    58441 tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc
    58501 tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt
    58561 ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt
    58621 tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc
    58681 ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac
    58741 ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc
    58801 caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc
    58861 ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa
    58921 gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac
    58981 cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt
    59041 gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt
    59101 gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt
    59161 gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat
    59221 ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac
    59281 gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct
    59341 ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt
    59401 caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt
    59461 cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag
    59521 ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa
    59581 ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga
    59641 tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc
    59701 ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga
    59761 ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta
    59821 cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac
    59881 gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg
    59941 aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac
    60001 gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt
    60061 gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt
    60121 catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct
    60181 tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga
    60241 ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa
    60301 tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga
    60361 ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt
    60421 cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat
    60481 ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg
    60541 tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt
    60601 ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat
    60661 ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat
    60721 ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta
    60781 gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac
    60841 agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa
    60901 ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc
    60961 cctttgacta atattgacct taattataac gaatttgata atagaaagaa agaagaacgc
    61021 tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta
    61081 tc
//
LOCUS       DAYYHB010000005        59481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000005 DAYYHB010000000
VERSION     DAYYHB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.005"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..775)
                     /locus_tag="ACHA6B_000273"
                     /pseudo
     CDS             complement(59..775)
                     /locus_tag="ACHA6B_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
     gene            complement(1002..1544)
                     /locus_tag="ACHA6B_000274"
     CDS             complement(1002..1544)
                     /locus_tag="ACHA6B_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8947882.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1549..1776)
                     /locus_tag="ACHA6B_000275"
     CDS             complement(1549..1776)
                     /locus_tag="ACHA6B_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8947883.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1855..3735)
                     /locus_tag="ACHA6B_000276"
     CDS             complement(1855..3735)
                     /locus_tag="ACHA6B_000276"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8947884.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3924..4538)
                     /locus_tag="ACHA6B_000277"
     CDS             complement(3924..4538)
                     /locus_tag="ACHA6B_000277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8947885.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4680..5240
                     /locus_tag="ACHA6B_000278"
     CDS             4680..5240
                     /locus_tag="ACHA6B_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8947886.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5560..5859)
                     /locus_tag="ACHA6B_000279"
                     /pseudo
     CDS             complement(5560..5859)
                     /locus_tag="ACHA6B_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6113..7748)
                     /locus_tag="ACHA6B_000280"
                     /pseudo
     CDS             complement(6113..7748)
                     /locus_tag="ACHA6B_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8071..8550)
                     /gene="rlmH"
                     /locus_tag="ACHA6B_000281"
     CDS             complement(8071..8550)
                     /gene="rlmH"
                     /locus_tag="ACHA6B_000281"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8947887.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            8978..10267
                     /locus_tag="ACHA6B_000282"
     CDS             8978..10267
                     /locus_tag="ACHA6B_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8947888.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(10306..10911)
                     /gene="ytpR"
                     /locus_tag="ACHA6B_000283"
     CDS             complement(10306..10911)
                     /gene="ytpR"
                     /locus_tag="ACHA6B_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8947889.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(11116..11580)
                     /locus_tag="ACHA6B_000284"
     CDS             complement(11116..11580)
                     /locus_tag="ACHA6B_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8947890.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(11602..11922)
                     /locus_tag="ACHA6B_000285"
     CDS             complement(11602..11922)
                     /locus_tag="ACHA6B_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8947891.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(12066..13145)
                     /gene="pepA"
                     /locus_tag="ACHA6B_000286"
     CDS             complement(12066..13145)
                     /gene="pepA"
                     /locus_tag="ACHA6B_000286"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8947892.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13345..13695
                     /locus_tag="ACHA6B_000287"
     CDS             13345..13695
                     /locus_tag="ACHA6B_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8947893.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            13918..14865
                     /locus_tag="ACHA6B_000288"
     CDS             13918..14865
                     /locus_tag="ACHA6B_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8947894.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(14922..16340)
                     /gene="pepV"
                     /locus_tag="ACHA6B_000289"
     CDS             complement(14922..16340)
                     /gene="pepV"
                     /locus_tag="ACHA6B_000289"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8947895.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            16489..17334
                     /locus_tag="ACHA6B_000290"
     CDS             16489..17334
                     /locus_tag="ACHA6B_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8947896.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17338..17778
                     /locus_tag="ACHA6B_000291"
     CDS             17338..17778
                     /locus_tag="ACHA6B_000291"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8947897.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            17828..18022
                     /locus_tag="ACHA6B_000292"
     CDS             17828..18022
                     /locus_tag="ACHA6B_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947898.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            18158..18844
                     /locus_tag="ACHA6B_000293"
     CDS             18158..18844
                     /locus_tag="ACHA6B_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8947899.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QVKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(19252..19941)
                     /locus_tag="ACHA6B_000294"
     CDS             complement(19252..19941)
                     /locus_tag="ACHA6B_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947900.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(19916..22045)
                     /locus_tag="ACHA6B_000295"
     CDS             complement(19916..22045)
                     /locus_tag="ACHA6B_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8947901.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(22093..23430)
                     /locus_tag="ACHA6B_000296"
     CDS             complement(22093..23430)
                     /locus_tag="ACHA6B_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8947902.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(23682..24041)
                     /gene="rplT"
                     /locus_tag="ACHA6B_000297"
     CDS             complement(23682..24041)
                     /gene="rplT"
                     /locus_tag="ACHA6B_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8947903.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(24094..24294)
                     /gene="rpmI"
                     /locus_tag="ACHA6B_000298"
     CDS             complement(24094..24294)
                     /gene="rpmI"
                     /locus_tag="ACHA6B_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8947904.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(24366..24890)
                     /gene="infC"
                     /locus_tag="ACHA6B_000299"
     CDS             complement(24366..24890)
                     /gene="infC"
                     /locus_tag="ACHA6B_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8947905.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(24934..25059)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            25210..25842
                     /locus_tag="ACHA6B_000300"
     CDS             25210..25842
                     /locus_tag="ACHA6B_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8947906.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(25844..26749)
                     /locus_tag="ACHA6B_000301"
     CDS             complement(25844..26749)
                     /locus_tag="ACHA6B_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8947907.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(26834..27493)
                     /locus_tag="ACHA6B_000302"
     CDS             complement(26834..27493)
                     /locus_tag="ACHA6B_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8947908.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            27809..28753
                     /gene="mvk"
                     /locus_tag="ACHA6B_000303"
     CDS             27809..28753
                     /gene="mvk"
                     /locus_tag="ACHA6B_000303"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8947909.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            28746..29723
                     /gene="mvaD"
                     /locus_tag="ACHA6B_000304"
     CDS             28746..29723
                     /gene="mvaD"
                     /locus_tag="ACHA6B_000304"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8947910.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            29736..30821
                     /locus_tag="ACHA6B_000305"
     CDS             29736..30821
                     /locus_tag="ACHA6B_000305"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8947911.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            30818..31882
                     /gene="fni"
                     /locus_tag="ACHA6B_000306"
     CDS             30818..31882
                     /gene="fni"
                     /locus_tag="ACHA6B_000306"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8947912.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(32131..32934)
                     /locus_tag="ACHA6B_000307"
     CDS             complement(32131..32934)
                     /locus_tag="ACHA6B_000307"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8947913.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(32954..33796)
                     /gene="kduI"
                     /locus_tag="ACHA6B_000308"
     CDS             complement(32954..33796)
                     /gene="kduI"
                     /locus_tag="ACHA6B_000308"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8947914.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(33812..34819)
                     /locus_tag="ACHA6B_000309"
     CDS             complement(33812..34819)
                     /locus_tag="ACHA6B_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8947915.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(34826..35470)
                     /locus_tag="ACHA6B_000310"
     CDS             complement(34826..35470)
                     /locus_tag="ACHA6B_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8947916.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(35570..36328)
                     /locus_tag="ACHA6B_000311"
     CDS             complement(35570..36328)
                     /locus_tag="ACHA6B_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8947917.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(36455..37606)
                     /gene="fucO"
                     /locus_tag="ACHA6B_000312"
     CDS             complement(36455..37606)
                     /gene="fucO"
                     /locus_tag="ACHA6B_000312"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8947918.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(37688..37999)
                     /gene="rhaM"
                     /locus_tag="ACHA6B_000313"
     CDS             complement(37688..37999)
                     /gene="rhaM"
                     /locus_tag="ACHA6B_000313"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8947919.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(38001..38846)
                     /gene="rhaD"
                     /locus_tag="ACHA6B_000314"
     CDS             complement(38001..38846)
                     /gene="rhaD"
                     /locus_tag="ACHA6B_000314"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8947920.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(38860..40116)
                     /gene="rhaA"
                     /locus_tag="ACHA6B_000315"
     CDS             complement(38860..40116)
                     /gene="rhaA"
                     /locus_tag="ACHA6B_000315"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8947921.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(40113..41573)
                     /gene="rhaB"
                     /locus_tag="ACHA6B_000316"
     CDS             complement(40113..41573)
                     /gene="rhaB"
                     /locus_tag="ACHA6B_000316"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8947922.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            41726..42595
                     /locus_tag="ACHA6B_000317"
     CDS             41726..42595
                     /locus_tag="ACHA6B_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8947923.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(42721..43620)
                     /locus_tag="ACHA6B_000318"
     CDS             complement(42721..43620)
                     /locus_tag="ACHA6B_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8947924.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(43785..44609)
                     /locus_tag="ACHA6B_000319"
     CDS             complement(43785..44609)
                     /locus_tag="ACHA6B_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8947925.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(44620..45915)
                     /locus_tag="ACHA6B_000320"
     CDS             complement(44620..45915)
                     /locus_tag="ACHA6B_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8947926.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(45931..46410)
                     /locus_tag="ACHA6B_000321"
     CDS             complement(45931..46410)
                     /locus_tag="ACHA6B_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8947927.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(46416..47444)
                     /locus_tag="ACHA6B_000322"
     CDS             complement(46416..47444)
                     /locus_tag="ACHA6B_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8947928.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(47461..48567)
                     /locus_tag="ACHA6B_000323"
     CDS             complement(47461..48567)
                     /locus_tag="ACHA6B_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8947929.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(48796..51393)
                     /gene="adhE"
                     /locus_tag="ACHA6B_000324"
                     /gene_synonym="adhC"
     CDS             complement(48796..51393)
                     /gene="adhE"
                     /locus_tag="ACHA6B_000324"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8947930.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(51698..52078)
                     /gene="yajC"
                     /locus_tag="ACHA6B_000325"
     CDS             complement(51698..52078)
                     /gene="yajC"
                     /locus_tag="ACHA6B_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8947931.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(52175..53320)
                     /gene="tgt"
                     /locus_tag="ACHA6B_000326"
     CDS             complement(52175..53320)
                     /gene="tgt"
                     /locus_tag="ACHA6B_000326"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8947932.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            53537..54235
                     /locus_tag="ACHA6B_000327"
     CDS             53537..54235
                     /locus_tag="ACHA6B_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8947933.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(54403..57042)
                     /locus_tag="ACHA6B_000328"
     CDS             complement(54403..57042)
                     /locus_tag="ACHA6B_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8947934.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(57661..58692)
                     /gene="queA"
                     /locus_tag="ACHA6B_000329"
     CDS             complement(57661..58692)
                     /gene="queA"
                     /locus_tag="ACHA6B_000329"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8947935.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            58885..>59481
                     /locus_tag="ACHA6B_000330"
     CDS             58885..>59481
                     /locus_tag="ACHA6B_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8947936.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT        17182 a        12623 c        10675 g        19001 t
ORIGIN      
        1 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
       61 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
      121 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
      181 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagttatggt tctgtctctg
      241 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
      301 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
      361 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
      421 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
      481 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
      541 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
      601 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
      661 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
      721 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
      781 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
      841 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
      901 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
      961 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     1021 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     1081 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     1141 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     1201 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     1261 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     1321 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     1381 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     1441 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     1501 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     1561 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     1621 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     1681 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     1741 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     1801 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     1861 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     1921 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     1981 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     2041 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     2101 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     2161 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     2221 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     2281 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     2341 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     2401 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     2461 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     2521 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     2581 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     2641 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     2701 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     2761 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     2821 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     2881 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     2941 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
     3001 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
     3061 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
     3121 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
     3181 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
     3241 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
     3301 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
     3361 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
     3421 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
     3481 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
     3541 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
     3601 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
     3661 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
     3721 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
     3781 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
     3841 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
     3901 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
     3961 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
     4021 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
     4081 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
     4141 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
     4201 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
     4261 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
     4321 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
     4381 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
     4441 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
     4501 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
     4561 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
     4621 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
     4681 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
     4741 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
     4801 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
     4861 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
     4921 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
     4981 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
     5041 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
     5101 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
     5161 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
     5221 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
     5281 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
     5341 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
     5401 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
     5461 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
     5521 tccagtttat tgaattcttc tcgtttacct caattcattt taaaggggaa tcagctcatc
     5581 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
     5641 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
     5701 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
     5761 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
     5821 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
     5881 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
     5941 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
     6001 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
     6061 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
     6121 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
     6181 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
     6241 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
     6301 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
     6361 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
     6421 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
     6481 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
     6541 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
     6601 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
     6661 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
     6721 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
     6781 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
     6841 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
     6901 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
     6961 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
     7021 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     7081 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     7141 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     7201 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     7261 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     7321 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     7381 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     7441 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     7501 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     7561 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     7621 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     7681 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     7741 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     7801 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     7861 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     7921 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     7981 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     8041 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgccc ccgcgttgat
     8101 ccggaaggca cggtagattt gctcgacgag gatcaggcgc atcaattgat gtggatacgt
     8161 cattttgcca aaagagatct gagcattgct tcttttcatg acggcttcgc tgagtcctaa
     8221 agaaccgccg ataacaaaaa caaggtggct ttttccttgg atacctaatt ggtcgatttg
     8281 tttagcaaaa gattcgcttg tgggattttt cccttcaatt gctaagccat agacgtattc
     8341 ttgttctttg attttcgcta agatccgttc tccttctttt tcttttactt gtctcatttg
     8401 cgcttcgctt aaattctccg gggctttttc gtcaggaact tcgatcaatt cgattttcgc
     8461 ataggcattc agccgtttga catactcatt gataccttgg atcaagtatt tttcttttaa
     8521 ttttcctact gaaataattt tgatattcat gttttctctc ctttgatctt cacgctcact
     8581 ttgtttatta taatcaattt tttcgtctat atgtttattt tgactatttt tgttaaaaag
     8641 atttccccaa cagttatgca caacatccac aggtttatcc acagaaaaca gcgttgtttt
     8701 accttatttc atcacattca ttaggcaaac atacgtttta taagaattta tccactttat
     8761 acacaggctg tggatatgtt ttatccctta tttttccaat cctatccgcg tagttttaca
     8821 caatatctgt ggaaaaagaa aatcaaatat tttcatctga aaacaatgat ttttcctaaa
     8881 gtttttcata aaaagttcaa agtctcaaag tagactaaag tcaacaaata agactatact
     8941 aacagtagat aaatgaatgt aattggagga ggaacacatg gatagaaaaa atgtgacacc
     9001 aaagatgaaa aaaaataaaa ataatagcct ctggcgtaag ttgggtctgg ggcttgttgg
     9061 agggatcgtt ggaggactac tgacagctgg cattttttat gcagcgatgg gcactggcaa
     9121 caatagttct actgctacaa gcggcaatca gaattctgcc ggtgaaacag ttgtggaaaa
     9181 tgtcaaagtc aatgtagatt ctgatatcac caaagcggta gataaagttc aagacgctgt
     9241 cgtttctgtt atcaacttac aaagtcaaaa ccaatcaagc ggttttggcg gactattcgg
     9301 gcagcaagaa gaaagtagca gctcagatga tagtaatttg gaagcttata gtgaaggaag
     9361 cggcgtgatc tacaaaaaag acgggaatac tgcttatgta gtcacgaata atcacgtagt
     9421 agatggccag caaggtcttg aagtcttgat gaaagacggt acgaaggtaa aagctgaatt
     9481 agttggaaca gatgcttatt cagatttagc ggtgttgaag atcaattcag acaaagtcga
     9541 aacagttgcc tcttttggtg actcaagtgc tctgaaggtt ggtgagcctg cgattgcgat
     9601 tggttcccca ctaggatctg aatatgcaaa ttctgtcact tcaggaatca tttcttcctt
     9661 gaaccgacaa gtgacaagta cgaatgaatc gaacgaaaca gtcaatatca atgcgatcca
     9721 aacagatgca gcaatcaacc ctggaaattc tggtggtcct ttagttaata tcgaaggaca
     9781 agttatcggt atcaactcaa gtaagatcgc aagtacttct gaatctactt ctaatgtaag
     9841 cgtagaagga atgggatttg ctattccaag taacgatgta gtgaatatca tcaatcaact
     9901 ggaaaaagac ggaaaagtga cacgtccagc tctaggtatc acaatggtcg acttatctgc
     9961 cgtttctact cagcaacaag aacaaatctt gaagattcct tcatctgtaa ctaacggtgt
    10021 catcgtgaca aatgtccaaa cagctacacc ggctgaaaaa gctggattga aacaatacga
    10081 cgtcattact aagatcgatg acaaagaagt ttcttctggt gtagaacttc aatctgcttt
    10141 gtataagaaa aaagtgggag attctgtcaa aatcacttac tatgaaggct cgaaagaaaa
    10201 aacaactact gttgaattga cgatcgatca atcggcatta aaacaaaatc aaaatagtga
    10261 aaattaataa aaaagaggtt gtgacaaaga cttgtcacaa cctctttatt ttggaaatgc
    10321 ttctccgact acggcatcct caggcaatac taagattcct ttttttgctg gagcatttgg
    10381 caaatgcaat tcacgagcgg aacagatcat tccatagcta tctacccctc gcaaagttcc
    10441 tggccagatc atcattccat ctggcatcat agctcctggt tttgcaacga caactttttg
    10501 tccagcttcg atattgggtg caccgcaaac gatttgcaat actgtaccat tgtctacttc
    10561 tgtctcagta atcgataaat ggtcagaatc aggatgtggg acgcatgttt ttacgtaccc
    10621 gacaacgatc ttcggttcat gatcagcttc aagctgttct tcgaatcctg cttctttgat
    10681 caatcgattc aatttcgcta attcctcatc tgttaagctt acttggccgt tcccttcgat
    10741 tgttagatga tcggagatat ggaaaaagtt ccaagcaaca acttgacctt gttcatcttt
    10801 gatccgagca acattccatt tgcgttcaac agtgttttct gttcctttat cgtcggctat
    10861 aataaccatc aaggtgtctc cgacgtgtgc tttgttataa gcaaaaatca tgttctttcc
    10921 tccattatcc tttgctgaga tttatcttaa caaaaaattg cattcactgc atgaaatttt
    10981 tttcatgctt tatttccgaa gaagcctctt ctggaacacc atttattcgt aacaagggac
    11041 tgtgacaaga cttttgtcac agtcccttta ctgttttagt taattgatta aaaaggtcgt
    11101 acaagttcca cttgcttaat tacttggctg ttcttcttcg tcagaaacga tcaagacatc
    11161 acaaggtgaa tgacgtatca cgtagctggc tacacttcct gtcataaagc gttcgactgc
    11221 atttagacct gactgcccaa ccatgattag atcgatctca tatttttttg gcaatttgac
    11281 tgccatggat tcacgaacag aaccatatgc aataattcct tctatatttg tgtaatcaac
    11341 agatttccca tattctttac attcttcgat catttccttg gctgtttcag tttgctgatc
    11401 gactagattt tgatcaatag gtgcatagcc cataaagtta taaaattgct gatcgattac
    11461 gtttgctaca tatactttcc cattattacg gcgagccact tctaccgctt ttttgaaagc
    11521 agcaagtgct tgttcactac cgtcgatacc tactaaaata ttcttatatt cttgattcat
    11581 cttgacccct cctaacagat gtcagccttt tgttacactt tctaggaacg attcgatttc
    11641 ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac
    11701 gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg
    11761 gtctacttca ataaattgcc aatcagaaaa gtcattttca atttcaggca tttgcggttt
    11821 gataaaacgg cagtctccgc accagccggc agtaaagaaa aatacactct ttccagtttc
    11881 tacgtagcct gctaattctt ctaaactatt tgggataatc attctttgtc ctctactttc
    11941 tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc
    12001 tctttttata aaaaaaatct ggcaaaaagc aatcttttgc cagattttat agtttcacta
    12061 tcgcattatt ttccgtatac gatcgtattt actgttgatt tatccaaacc tttcagtgtt
    12121 tgaatcaaca tttctcgtgc agcttcaaaa tcgcgaatac tgaacatcgt ttgatgcgta
    12181 tggatataac gtcccactac gccaatcact gtacttggga ttccttcgtt ttgtgtatga
    12241 gcagcacctg catctgtacc gccttttgaa acaaagtatt gataagggat attgtttgtt
    12301 tcagcgatat ccagcaagta ctcgcgcaaa cgaggaagca tgatcaaacc tggatcttgg
    12361 atacgcatca atgtaccttc tcctaaatgt ccgaatgtgc catttttcgt cactgtatca
    12421 tctgctgcag agcagtctac tgcaaagaac agatctggct tgaatttatt gacagaagct
    12481 ttagaaccac gcaagcctac ttcttcctgg acattcgcgc ctgcaatcaa tgtatgacct
    12541 aatgtttcat tttgcaacgc ttctaaagct tctaatacca ttgtacagcc atagcggttg
    12601 tcccatgatt tactgataat attctttccg ttggctgttt tgatcgtttc agcgtaagga
    12661 acgattgtgt ctcctggaag gacaccgtaa ctcattgcct cttctcttga ttcaaaccca
    12721 gcatcgaaca aaatatcggt tacttctact gatttttgtc cgcttgttcc tcgcaataaa
    12781 tgtgggggaa cggaagaaga aatacatgga taattcttac cagtagaggt tttaagtgta
    12841 aaacgctgag cagaaacaac gtaaggattc cagccgccta gaggtacgac tttaaaaagc
    12901 ccgttttctt ggatttgtgt cagcatgaat ccgacttcat ccatatgtgc tgcgaccatt
    12961 acacggggtg catccgcttc ttcatgatgg cgcagaccaa aaatgccgcc caaaccatct
    13021 aactgtaact catctaccag cggttccata tgactttcca tataagcgcg aacatcttgt
    13081 tcaaaaccgc ttgttccttg taattctgtt aactctttga ttcgttgaaa tgttttttct
    13141 tccatatgct attcgtctaa atttcagact aaactcctct cagacttata ttgatctgcg
    13201 gataaaatat gattttttct gcagaccgat cgtatttatt atagcttatt ttttctggta
    13261 gcggttattt atttcaaaga aaatatgaga gtgtggtaaa ataagcacaa ggagtaacaa
    13321 gtgagaagaa ggaggtatgt ccacatgtat gaagaaagcg aactaagcta ttttaaaggc
    13381 gggttagcag ttggaatcgg tctaggatta ttgagcgggg tagcttcaac gatttggtat
    13441 catcgcaaaa aagtattgtc agcagaccaa gtacttgaaa atatcaagtc atcttttcta
    13501 aaagaaggtg caatcgaagg ttcttggatc tcttttgaaa aggaacctat taggaaattc
    13561 gccgttcggt cgagtgctta tcggggaggt ctttctcgaa tagaagatgg cgaaactgtt
    13621 tattatgaat ttttagcaga tgctcataca ggaaccgtat tagaaatttc aagaaaaaaa
    13681 ggtgcaacag attaatacgt caaaaaagtg agagaacgaa tacgcaccca ctattcaaaa
    13741 gagaggtatc atgtccttct tttgtagtgg gtggtttttt agatagagct ttccaaacat
    13801 atattccagt attttctttt taaataaatg atatttagcg ggaaaaacta gcatttcctt
    13861 tgattatgag gtatttttaa tttagctttt gttttctata gttaaggaga gtgaaaaatg
    13921 gaacaacaag aaaaaattgc tgttctgatt ccttgttaca atgaggaagc aacaatttca
    13981 acagtcattg cagactttaa acgagaatta ccagaagcag atatttatgt ctatgataat
    14041 aattcgaccg atcgaacata cgaattggcc gttcaaggtg gagcaatcgt aaaaaaagaa
    14101 cctcgtcaag gaaaaggaaa tgtgatccgc caaatgttct ttgatatcga tgccgactat
    14161 tatttaatgg tagatggcga tgatacttat cctgcagaag ctgtacatgg tttgctcgag
    14221 aaattacgtt ctggtgaagc agatatggtc attggtgatc gcctatcaaa tggaacctac
    14281 tttgatgaga ataagagacc tttccacgat tttggaaata accttgttag aaatacaatt
    14341 actagaatgt ataaaacaaa aattcttgat gtcatgaccg gctatcgagg attcaatcgt
    14401 atctttgtca aaagttttcc tattatgagt tctggttttc aaatcgaaac ggaattgacg
    14461 atccatgctt tagataaaaa attcaagttc gttgaaatcc caatcgatta tcgtgatcgt
    14521 cctgaaggca gcgaatcaaa attgaataca ttctctgacg gattcaaagt gatcatgatg
    14581 attattaaaa tgtttaaaga ttataagcct ttgcttttct ttgggatttg gaccatcttc
    14641 ttctttttat ttggattggt cacggggatt cctgtgattc gcgaatatat gctgacaagc
    14701 tttattacac gcatcccttc tgcgatttta agtactggat tgatgacttt agcacttctt
    14761 tcattagtaa cagggttgat tttggataca gtcgtcacga acgctaagaa agaatatgaa
    14821 ttgaatctat atcatgttta tcatgaatac cacagtagaa agtaaaaaaa ggatcaagac
    14881 tgcaaaaata atttgcaatt cttgatcctc tttttaatta attatttcgc caaacgatag
    14941 atggcatctg catagattgc tgttgctctg aacaagtcat caagactcat aaattcgttt
    15001 gcttgatgca ttgtatcagt atatcctggg aacatcgctc cataagcaac gccacgtttc
    15061 aacaaacgtc cgtaagttcc gccaccgatg atttgttcgt agcctttttc tccagtgtgg
    15121 tcttcataga cttgcaataa tgtagccacc aatggatcat ccattggtac gtaatgtggt
    15181 tccattacgc gagcacctcg agtaactgtt gcgccttctt gccccactgt ttgttcgatg
    15241 tcactttgca atccgtctac tgttacacct tttggaaaac ggaaattcat attgatgaag
    15301 ttcgcttcct ctgtaccatt ttcttcaaaa gcaaatagtc cagcattcat tgtcaatttg
    15361 cccatttttt catcttcata agcgactcct agtttttctc cataaaaatc ttcatggaca
    15421 taagcagctg ctacatggat aaattgttta gcagatccta ggaattcata gttatctaag
    15481 aacaatgcca agaaagaacc tgcattgata ccagattgtg ggcttgcacc atgtgcgcct
    15541 ttaccaacta attcgatttt tacatacgta ttgtccgctt caatcgttcc gcttactgga
    15601 tttgcttcga tgaattggta aaatgcttct tccatcgcga tagctgcatc tgcacttggt
    15661 acttgaagtg ctgcagttgc tgttcctggc accatatttt cgcgaagtcc ggaaacaaaa
    15721 gatttcaaga cataatctcc tgcattgtcg cctttgaagc gtaaagctaa agaaacattt
    15781 cctttttcac cattgatgat tgggaattca gcatctggtg agaaaccaaa atctggttct
    15841 tcttcatgtt cgaaataata agccatatcg ccccagccgc tttcttcatc gctgccgact
    15901 acgaagcgaa ctttttttga tagtttcaag tcaagttctt taatgatctt catcgcatag
    15961 taagctgcca tacttggacc tttgtcatcg cttgatccgc gtgcaaagat ctttccatcc
    16021 ttgattactg gttcataagg atctgtgtcc cagccatctc ctgcaggaac gacatccatg
    16081 tgtccaaata tccctagcgt ttcgtctcct tcgccaagat caatatgccc tgcatagtta
    16141 tcgacatttt ttactttaaa tccatcacgt tcgccataag ctaacatatg tttcaatgcg
    16201 tcgcgaggtc caggtccaaa tggtgcatct ggtgttactt ttgagtcatc gcgctcgctg
    16261 ttgacacgta aaagattttt caagtcttca agcaagtctt ctttacgtgc ttctacttct
    16321 ttttgccaat caattgtcat aaagttaatc ctccacttat ttaagtcttc gtgttcatga
    16381 taccacaaat tacagaaaaa aatagttcaa aaaatctgac aattcctttt cttatctgtt
    16441 tttctctaaa aaaaatatac gctataatga gtaaaaggag gtttttagat gctgataaac
    16501 gaacaactag tgaaaagagt catcacacct cgtccaaaaa tttcacataa aggaacattt
    16561 ggacgtatcg ttttgattgg cggcaatgaa caatatggcg gagcaatcat tatgagtgct
    16621 ttagctgccg tacacagcgg agcgggtctc acgactgtag taactgatga aaaaaatcgg
    16681 agtgctcttc acagtcatct gccagaagca atgattgtag tttggacgga tactgaccag
    16741 tttctttccg taataaagca agcagatgta ctcttgattg gtcccggatt gggtacttct
    16801 tctaagagtt tggaacgttt caaacaagta ctagaaaacc aatcaaaaga acaatggctg
    16861 gtgattgatg gctctgcttt aagtttactt gctgctaatc atcttttgct accctatccc
    16921 gaacaaactg tacttacacc tcatcaaatg gagtggcaac ggttgagttc tctaccgata
    16981 caagaacaac gggataaaga aaacaaaaag aagcaacagg aaatcggtgc tgtgctcgtt
    17041 ttgaaaagtc atgagacaaa aatttatggt caccagcact tctacaccaa tcctttaggt
    17101 tctgctgcta tggcaacggg cggcatgggt gacactttag ctgggatgat cacaggattt
    17161 ttagcccagt ttcctcaaaa tgaccaaacg attgcagcag ctatttatct tcatagtttc
    17221 attggagacc agctggcaaa agaacaatat attgttttgc cgacacaaat cagcgaagca
    17281 cttccgaaat ggatgaatta tttttgtaaa aaatcaatgg aaaacgaggc atgagtcatg
    17341 gagaaattta caggttatgt gcgagaacct ttttattatg aaacagatca aatggggatt
    17401 attcatcaca gcaattatat ccgctggttt gaagaggctc gggtagcctt attggtacat
    17461 ctagggtttc cctataaaaa aatcgaagaa tctgggatca tcatccccgt tctggaagta
    17521 aattgcagct ataaagaaat gatccattac ggagaaaaag tgagcatcca tccaactatc
    17581 caaaagtata cggggacacg gttggatttt agctacgaaa ttgtgggaac tgaagacaaa
    17641 aaattaaaaa ctactggcag tagtaaacac tgttttttac tttctgattc gcaacgatta
    17701 gtgaagctga gcaaagtcaa tccggagtta gatcagttat tccaagcgta cgcttctttg
    17761 gatgatgtaa attcttaata aagaccgtct aagcattttt cttgtattgt gtcgagtagt
    17821 tcgtataatg aaaagaaaaa agcaggtgat ccccatgtat gtcgttaaag taatgcacgg
    17881 ttacatcgat aaaactggct gtcgcactcg ggaaaaaaac ttagacaatc tgctcatttt
    17941 caaagacaaa aaagaatccg aagcttttgc taaaagaatc ggcggtcgtg tcaaaccgat
    18001 ccaagaggta cgaccagatt gattcaatat ttttcttttc gaggctctaa taggcatgcc
    18061 tatcagggct ttttttgcag actgtgtttt tatgcccaca cgatttgtaa gcgatttatg
    18121 ttataataga ttatatctgc atagaaagga aggttaaatg gttatcaata atttgcaaca
    18181 gcaagcttat gaatctatac gtaaacaaat catttattcc gagttagcac ctggaagtaa
    18241 aatctccgat aaagtgttag aagaacaact agctatcggg cgtacaccga ttcgagaagc
    18301 actgatccaa ctgcgcaatc aagagcttgt aaaaaccatt ccacagtccg gcacttatgt
    18361 ttcaaaaatc gatatccgat ctgcttcatt agcccgttat acgcgggaaa agttggaaaa
    18421 accgatcctg caagaatgca gtgcgaaaat gaccaagcaa gatcaagtta aactagaaaa
    18481 aatactagaa caaaccgatc aggcagtaat cgctcaaaat aaaaaagctt tttttgattt
    18541 ggatactgat tttcaccgaa cttgttatga aattgctgga aaacgagaaa tttgggattg
    18601 gctagaaagt tatagtacgc acttgaaccg tttcagatgg ttacgtttga cgatttcaga
    18661 attagactgg ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa tgattgatca
    18721 taactttgat gaagtcggat ttctttgcac gatgcattta catatgatta tcgaggagca
    18781 agaatacgtg attcataatt accccgatta ttttgaagat attcaggaca gacccatcaa
    18841 ataagaggtt ttggaatcag cgttttgacc tgagtattag agaaagaaac ggcaaaatag
    18901 ttcctcatat tttgccgttt tttttgagct aataccgagg gtcagctgat tgcacaccgt
    18961 ttattgagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
    19021 tagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    19081 cgttttccaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa
    19141 aaacagcttc tcatgtttgt tgaatatttt gacttgtgac cgaggagttg acaggagaac
    19201 accgtttatt cggaaaaaaa ggactgtgac acgacttttg tcacagtctc tttatttgta
    19261 atctaaatca tactccgtac atcttttttc tatttttttt atttcttcta agatcccttg
    19321 gctacgaatt tcattatatg gccaaagtaa gactgcctct aatacataag gcgaaaaatc
    19381 gctttgattc atctcttttg aaaaactcat cgctgctatc tcgctcaatg cacgaatgtt
    19441 tgattgatgt gtataaaaag gcaaccgcca cagcacaatt ttttcgcttt gcgtataaat
    19501 tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt ccaataatca aagcttgcgc
    19561 tgtttcttta gagatcgaag ctagtcctga acattttaat ttttctaacg gctccgtcat
    19621 caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg attcgttcgt ttatttgctc
    19681 tgattcacag gaaacgattg gaacagccat ttgttcacaa atttctccta catcggcaaa
    19741 agggtacttt tttttgatgc tacatgccgt ttcgatacca caatcgattg attgttgtat
    19801 aagaattttc tgcttggaca aaggaatctt tttccaaaat aaatctcgcc gaaactggta
    19861 gaatccccaa atttcttcgg ggatctgttg taattcgttt agtaactctt ctaacttatg
    19921 tttgtttgat atttccggca acgacgatca cttcttcctc cggttccata gtagatactt
    19981 ccagatccat caaaagctgt agttgttcca gatccgtcgc tgtaaaatca gagactcttg
    20041 ctcctaagca gagataaaat tctggtcggg caatcaattc agtttgataa actgccatat
    20101 cttcccaaag ttgttcgaca gctgtcatat aattggctgc agttgttttc atacatgaag
    20161 catgtccacg ttggaccttg ataaatcctt tttgatcgat gatccgaaga cttccagcat
    20221 tttttgaacg attctgttcg ccgcatacat aaaagaaagg tgtattttcc aataagcaaa
    20281 cttcattttt atttaatcgc atatcctctg cagcaagctc tagtgcttct tctgttgtga
    20341 tctccttggt tagatcggtt gctttcactt ccgtagaacc agtagcgata gccgttactt
    20401 ttgaagtctg tggatcgatt tctacgtgga cttcgatcga ttctggtgta gcgcctgatt
    20461 caatcgcttt attcatcgct tcatttttca acgatcgtat atcttcttta cttggcgaag
    20521 ggatgatacg ttctacaaca tctcggacca ttgcaagtgc tacaccaata gaagaaatca
    20581 cttcggcgtt ttcaggaatc gaatatttca cacccatttt attactaaag taagtaatca
    20641 atgaggcagc gccgccccct acacctacaa gagaaatctg atctttctcc aagtgatact
    20701 tgtctgccaa ttctaaaatc accggttcaa tcttagcata ggatttttcc atgatctgtt
    20761 ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa tgcttggatg gcttttcgtg
    20821 cagatgggac atttccataa gaaaaatgtt cttcttgcac taatccaagg acattggctg
    20881 cacatgtatt tgtaatagtc acttcttctc cgtcttccat cactaccttg acgtaatcag
    20941 cagggtctcc ttctttagga gagaaaaatt caaccttagg acctttgatt ttttctgtct
    21001 ccgtaaatac tgcataatct agaccggcaa tatgtgcaga acgtggaccg acatcgatga
    21061 ttcctgactg attagcccgt accatcgaac cgcctgccac acctagaacc cgaacatcta
    21121 atgaagagat atatgttggg tggccaccta caattgaata atcgattgct ggacgtccat
    21181 ttttaatgac cccgatattg gttgttgttc cgcctacttc aaaataaaca ccattagacg
    21241 ctctcaagta catcaaagag cccatgactg atgctgctgg accggaaagc atggttaaaa
    21301 caggtcgttt tttcatttca ttgatttcca ttactccgcc gtctccgcgc ataatcatca
    21361 aagaaacatt gacccctgct tctcgtacgg aatcttctgt agaggttgcc gtatctagca
    21421 tttttggtaa aatagatgca ttgattgctg ctgtccgagt tcttcttgtc aatccatata
    21481 attttgtgat gtcagaagcc atcgttgtag ggatcgattt taccgatgct gcttcataaa
    21541 caactcgttc tggttcccca ttatctactc caaatgccat agaagaaacc aagacctccg
    21601 ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg gtctacattt aagtgtttga
    21661 caggtaagaa tgcattgaca atctctattt ttttcttatt ccctaaatcg atatcgttca
    21721 atctagtttg tcgttttgct aaaaaacctt ctaatccacc ttttgccatc ccgattacgc
    21781 cgacttttgc tacgtctcct tcaatcagtg cattagtcgc ttgtgtggta gaatgtgcaa
    21841 caaacacaac atcttctgga ctgatattat tttccagcaa acaattttga aaagactgaa
    21901 cgacacctgc ggctactcca cggacatcat cgtgggttgt tttcactgaa gattttccga
    21961 tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt atgtgtaccg ccaacatcaa
    22021 ttcccattcg cacaaaacgt ttcattttct tttcctccat tcatctaatc gtttttatcg
    22081 acgttttgtt tactaggcgt tcccgtacaa taagaacgtg atcaggcata agatcgctcc
    22141 gatgatccaa ccagttggga tggatttctt catatattct cgagaagtta cttttgtata
    22201 acctaatccc caagcaaccc aagattgtgt aatatctaaa tgctgaggag caatcgttgt
    22261 gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt ggattaacag ctaatacgac
    22321 agctaaaata gctgagcctg aaccgactag attcattgga ccgcggaaaa atcctagtgg
    22381 tgtcaatata gcaaatacga ttgccaagac taatggtgat tgcggtatcc aatctccaaa
    22441 aatagctgaa aaataaggag aagcatacgc agctgaatta ttgaacatcg ctaaggtcat
    22501 caagaaacca accattggcg caacatctac tgcgccatct gtaaataatt tcgcaaactg
    22561 acgacagatt tctttataac ctccgcgcaa ttttccagta aacagcaatg cccagatacc
    22621 tgctagaata aaacctaaga tgattggaag atctaaaaga acgaccccta aaactggtaa
    22681 taatacggct aaccatgaga tcattggtgc gtcgccacct tcagctggta cgtttgctgc
    22741 cattgcatag cgtttctttt tgttcattga tatattagcg accgttagta ccacaactaa
    22801 gcttacaatc atcccaatca taccaatttg gaaatagtca ttataagtat agctttcagc
    22861 cgctggattg aaaccagcat aaatcgtttg atattgtttg aaattgacga tattagcaaa
    22921 gattccagcc ataatcgagc ccataaatga gaacagtgca accggtactg aaatacctaa
    22981 tgacatcaag attggcaaca caataactgc aatagaaatc actggtccga ttcctgtcat
    23041 cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc ttggcttgtc
    23101 ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat ctactagaac
    23161 acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt actcagctgg
    23221 ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca tcgccgggtt
    23281 tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca tgatggcaaa
    23341 gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga atgagataac
    23401 taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt gtttttctga
    23461 taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg ctttcattaa
    23521 agggtgacca taaatgttca gttttcaatt aaaaaacctt aattatgcta ttttagactt
    23581 atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga ggaaagaaca
    23641 taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc caaagcttct
    23701 tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa gtcagctaac
    23761 attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt tgagtagctc
    23821 aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg gaagtcgcgt
    23881 ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg ttcttttgct
    23941 gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag cactctttta
    24001 cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc ctcctaataa
    24061 aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta gttgttggcg
    24121 aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac ggcgttgttt
    24181 ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca atccgccttt
    24241 accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg gcatgactat
    24301 atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc agttagtcat
    24361 tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg tccgtccatt
    24421 ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg attcaagact
    24481 ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga agctttcact
    24541 ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa gtcattcaag
    24601 tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt tttacgcgct
    24661 tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat tcgcgcaact
    24721 ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc tgcaatattc
    24781 aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat caatcgtaac
    24841 tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat tcacctccaa
    24901 atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg agttctctta
    24961 cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc cagtgacatt
    25021 atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca cagtatagca
    25081 aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct gaaaatgatt
    25141 agattagcag actttctagc agtgattccc gcttctttgc tatactaaag aaaaacgagg
    25201 tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct tacaaaggga
    25261 gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg gggtgaccaa
    25321 agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa acttacgatg
    25381 caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt
    25441 tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca atgtaaatta
    25501 acaggtacga attttgccga aagttatctt cgagactgta ctttcactga ttgtattgct
    25561 tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca ttgtcaatta
    25621 gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga caatcagttg
    25681 accggttcga actggtttcg aacttctctg aagggattag actttacgac taatttcttc
    25741 acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca agagcaagca
    25801 cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga atccgccgcc
    25861 acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg attccccatc
    25921 cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct gtcacaatag
    25981 ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt
    26041 tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa gctaagttcg
    26101 tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct ttttcctttt
    26161 ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact acattttgtt
    26221 ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc agcagattct
    26281 tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta ttcacttcct
    26341 cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat aaactgtata
    26401 cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg tactcggtat
    26461 tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata ggactagttt
    26521 cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt aacgcttcat
    26581 caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa gtcagcatct
    26641 cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg ttttgctgta
    26701 cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata ctctatcctc
    26761 ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt
    26821 gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat ttttcttcga
    26881 tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag cggtctccat
    26941 cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa cgtctgttca
    27001 attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac gaacgaccac
    27061 gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact aataaatccg
    27121 gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc agttcatcat
    27181 acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg aaaagcagtg
    27241 aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct tgtttgatgc
    27301 ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt tcagggtttg
    27361 cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc tgactatcaa
    27421 agtgttcagc catcatttct gttaaacttg attttccggc accaattgta cctgctaaaa
    27481 cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt aactccaacc
    27541 tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta gtcaaagaag
    27601 aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac gtattcttag
    27661 ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag acccttagtg
    27721 gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt tgcttaaaaa
    27781 tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag aatcaagcgg
    27841 aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga ttgcctttcc
    27901 tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg acgatcaact
    27961 ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa aaaatatcaa
    28021 aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc aactgaccat
    28081 tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg ccacagcagt
    28141 cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac tattagaaaa
    28201 tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg cagccgctac
    28261 tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact tttctttgaa
    28321 catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt
    28381 caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa
    28441 attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt
    28501 ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca gcaatgattt
    28561 tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca aattaactgg
    28621 cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag cccaagaaat
    28681 cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat taggagtaca
    28741 tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc aaatattggg
    28801 gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta acattggatg
    28861 cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat gaattttatc
    28921 tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt
    28981 ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat ttcgttccta
    29041 ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc gcttgcaata
    29101 ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga cgcgggtctg
    29161 gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact
    29221 taagttctta cgctaagcca gtcccttccg attctttcga agacgattta gcaatggttt
    29281 tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg cgtcggacag
    29341 tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat ctatatcaat
    29401 tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg gaaagaaacg
    29461 gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac tggtctcctg
    29521 acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt ccatgctact
    29581 ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat ttatctgagg
    29641 tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca catgctggtc
    29701 caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct gcaccaggca
    29761 aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca gttatcgctg
    29821 cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga agtatccaat
    29881 ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa ttagttttag
    29941 atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact gaaaagtatg
    30001 cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt gagttagaca
    30061 gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca
    30121 aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc aagattgcag
    30181 cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct gctagctgtt
    30241 atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa caagaaactc
    30301 agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt gagccattga
    30361 ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc tctacttctg
    30421 atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat cagcttttct
    30481 taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa
    30541 cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta tctgcaatca
    30601 ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct gaacagtatg
    30661 aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta attgttgacc
    30721 agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc actccactgc
    30781 cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt
    30841 ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg tacggatcat
    30901 ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac aggcagttgg
    30961 gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa
    31021 aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga ttgccactgg
    31081 ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga ttatgcgaca
    31141 agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg tcgaaagagc
    31201 caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga atgcacctca
    31261 agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac ttattcaaga
    31321 aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg gcatgacaag
    31381 agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca gcggtcgaag
    31441 cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt
    31501 agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag cagacacttc
    31561 tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta aagccctttg
    31621 tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga tgaatcacgg
    31681 cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc tgctttatac
    31741 tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct tttccggacc
    31801 agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa gacgcacaga
    31861 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtacaaacg
    31921 gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    31981 ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc ctcttttttc
    32041 gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg atttcacaac
    32101 ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat aggcaagtat
    32161 gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag aagccaagaa
    32221 gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg tacgtccaac
    32281 gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta atggagcagt
    32341 ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt attgaccgta
    32401 ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag cataagcact
    32461 gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt taccgccgcc
    32521 tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat ttaaatccac
    32581 gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc gtttgatgat
    32641 ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct gttgaaccat
    32701 cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat cgatgcctgc
    32761 ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat taaatacgat
    32821 ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat agacagcacc
    32881 ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt ccatcatttt
    32941 ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct gtataggtga
    33001 tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca cctgaatgga
    33061 tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt tttgtttcat
    33121 ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca aagtagacat
    33181 aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg cttcctggtt
    33241 ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga tggatgtatt
    33301 ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt tttacttggt
    33361 cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta atatagaatt
    33421 tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct tttccgatat
    33481 agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg aatcctggtc
    33541 caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca cctagttctt
    33601 tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag atcattcgat
    33661 cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct actaaaaatt
    33721 cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt gtataacgtg
    33781 tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca gagccgcatg
    33841 ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg atcgtatgct
    33901 tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata ttggataaaa
    33961 tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc acgattgggg
    34021 tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc cccttcaatt
    34081 cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga atattcggga
    34141 acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct tctggcgctt
    34201 tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata tctactaaag
    34261 gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt ccacgatagt
    34321 tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc tgtttcgtca
    34381 ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat atatctattc
    34441 cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa cttgatccgg
    34501 cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata taataagtac
    34561 ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat tgcaagtgct
    34621 ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc
    34681 cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg gcagcgaatc
    34741 gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg atttctccga
    34801 gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt gctgcatact
    34861 gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc
    34921 cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg tccagactca
    34981 caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca gaaatgatac
    35041 ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt ttcagcgctg
    35101 ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac agattgcaca
    35161 tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca gctaagatag
    35221 ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt tctggttggt
    35281 ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact gtaaaagtca
    35341 actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa gcttcttctt
    35401 tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat aaaatatcaa
    35461 tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa cttggaaatc
    35521 tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat tatttttgaa
    35581 ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt tcttttgtct
    35641 catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt tgttcgtttt
    35701 tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga
    35761 tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg gtgatcgtat
    35821 attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc tcttcactga
    35881 attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg attccgattt
    35941 ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac aagatctcat
    36001 tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc tgttctaaaa
    36061 acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg tagcgaataa
    36121 gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg atcatcaaca
    36181 aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt gcgatcgtct
    36241 gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct ttgatcaata
    36301 cagttccata aggcttttta ttttccatat aatattctcc tatttcttta taagaaaaat
    36361 tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc atttctcttt
    36421 acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga tataaagcaa
    36481 tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct cgcaaagcat
    36541 cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg gtttctggga
    36601 ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt acatcttcta
    36661 aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg tacttttctc
    36721 cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac gctacgccat
    36781 gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt cctaaaccga
    36841 catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct cgcccatttt
    36901 gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct tttaaatgca
    36961 gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca tgcgtcatag
    37021 catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata tcgctgtcga
    37081 tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg tgatttactt
    37141 catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc gttggtatag
    37201 ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta atgacgtctg
    37261 aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc
    37321 caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact ccatctttta
    37381 cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg attgctgctt
    37441 tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa
    37501 tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac tcaatcgctc
    37561 cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc tattcctcct
    37621 aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt tgcccagttg
    37681 cttgttgcta cagttcaaag actggcatca attcccaact gactggagag gcatctggat
    37741 ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat gtatttgcca
    37801 ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa cttgtactcg
    37861 gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta cgcatttctg
    37921 gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa actttcatgc
    37981 aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc caaataacct
    38041 gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc cgtgattgtt
    38101 tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt ttctgctgtc
    38161 tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc atgttgtggc
    38221 cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat ctcatttgtt
    38281 cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa gcattccgta
    38341 cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt aaaactcatc
    38401 gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc tacttctaaa
    38461 cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca ttcgttttcc
    38521 agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat tccaagattc
    38581 tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac aataaaatag
    38641 catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac tttttcagga
    38701 tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc gccatttctt
    38761 tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt cacataaggc
    38821 gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta attccaacac
    38881 gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa cttgattttt
    38941 gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt ccgtataagc
    39001 cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa ctggttccag
    39061 catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag cagcgatacg
    39121 gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca attcgttacg
    39181 gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat ggtctgaatc
    39241 ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag ctaatgaaga
    39301 taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac aaacaagttt
    39361 gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat aagcttcagc
    39421 gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat ctaactcttc
    39481 tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac gatcaatcgg
    39541 attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc cagtttcttt
    39601 cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt attcacgaac
    39661 ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg aaaagaaagt
    39721 cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt tttcaaaatg
    39781 tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga tagcatgaag
    39841 gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt gtctaagctc
    39901 ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac cggtcaattc
    39961 gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca tagaaatttt
    40021 gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg catatttttc
    40081 ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt gattttattc
    40141 acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc acatggaaac
    40201 gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt actgagcatt
    40261 tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac aatgattccg
    40321 gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc tacgatgtgc
    40381 agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg tgcgtaacaa
    40441 agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg gactggctgc
    40501 tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt caaaaatcga
    40561 gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag ctctgccaat
    40621 tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa ccacatcccc
    40681 atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt ataattttca
    40741 gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag cgaccatgtc
    40801 ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac agctgacgcc
    40861 gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt tgaaaattgt
    40921 tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa taattccttc
    40981 gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc attcagcagc
    41041 tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac ataccctaaa
    41101 taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc ttcagcaaac
    41161 aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata caacttatct
    41221 agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc ccgataagca
    41281 atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac tgcccatgta
    41341 tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa tccaatcaaa
    41401 atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt ccgaaagccg
    41461 tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct cgtacttttc
    41521 attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt catttctcca
    41581 cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat ttattgacaa
    41641 tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata taattgcatt
    41701 atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc actcatggtc
    41761 ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt tattttttta
    41821 tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac gagcacattt
    41881 accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat ataatgataa
    41941 tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat atcagcgtta
    42001 tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg gtcagaacgt
    42061 ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt caacaggaga
    42121 tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc aacaaggcac
    42181 gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga atgaactgat
    42241 tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg tttacaaact
    42301 gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat tagaacaaca
    42361 attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg gttctccacc
    42421 aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc ggtatacaga
    42481 atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt actttagccg
    42541 cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca gctgattcat
    42601 ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa taaaataacc
    42661 gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta gtgatcattc
    42721 tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat cacgtttcaa
    42781 aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat gcggataaga
    42841 agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca acaagtgctt
    42901 ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag tgtacttttt
    42961 aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt cctctaaaga
    43021 aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac gctcattagg
    43081 aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat tgagaaaaat
    43141 caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt ttttatcagg
    43201 aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt gcaatacacg
    43261 actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg gcgtggaacg
    43321 actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat ctgaagattg
    43381 gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg ctttgatcgt
    43441 aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat ggatctcata
    43501 gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga catgatagat
    43561 ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct tttggagcat
    43621 gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt ttttgtaatc
    43681 aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc ttctagcccc
    43741 gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc aagaagactt
    43801 cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag gtacggacga
    43861 ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc caaatttacc
    43921 ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca tatcttttca
    43981 ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc actacttgtg
    44041 atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc ttggatcagc
    44101 tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat ccccaccatc
    44161 atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc ttcagcaaat
    44221 gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca tccagtttcc
    44281 gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc gaactttgtc
    44341 agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca acttaaaatt
    44401 cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat cccagaattg
    44461 atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag ctgcagtgta
    44521 tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac ttcggaaaga
    44581 tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca tattgggtag
    44641 ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga taaccccata
    44701 aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac ttgcaccgac
    44761 atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca tgattgaaaa
    44821 caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa atattggtgt
    44881 aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata acacaagatt
    44941 gactaataac agaataataa atttattatc cgttacgccc ataatcaacg aactaattgc
    45001 ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag ccaataagaa
    45061 catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat cttttagttt
    45121 taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta tcgctgaagc
    45181 ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa tcaatagaag
    45241 gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt tttcgacaac
    45301 agggtattta ttctttttag caacgatgaa tgctactagc atgattccta gcgcccacaa
    45361 agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac cagcaacaag
    45421 tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag ctgacgcgat
    45481 attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag ggatcaacac
    45541 tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa ataaagcatt
    45601 ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa
    45661 atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga gaacgaaaaa
    45721 cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag ctgaactaaa
    45781 agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga atgcaatact
    45841 tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata acactaatcc
    45901 ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg ctggcttgtt
    45961 gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc ccgatacagg
    46021 tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct gccccattgt
    46081 caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata ccagaaataa
    46141 actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt cacttaaaac
    46201 cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca tggcaaacca
    46261 gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta aaaccgttct
    46321 ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag ctcccaggat
    46381 ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc ttggatcttt
    46441 tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg ggcaactgcc
    46501 tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc ttgtgctaaa
    46561 gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc
    46621 gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat ggcatcattc
    46681 atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt agccacacca
    46741 ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat caaattggat
    46801 tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc taaaagctct
    46861 accattttga tagcggtttc gctttgcatc acacggatct tctttccctt caaatcttcc
    46921 ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc ataataggtc
    46981 aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat ttcttcatta
    47041 tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga aaaaatcgaa
    47101 taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc tactgctcct
    47161 gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg gaaatactgg
    47221 atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt cattgcatca
    47281 attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc atttacatct
    47341 gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc tagcagacct
    47401 aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt ttcctttctt
    47461 ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact gtccataagg
    47521 cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc ctgtcccgat
    47581 tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt ggatagcacg
    47641 ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt tcaaaatacc
    47701 gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat ccaacaacgt
    47761 atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg cttcaagtgt
    47821 ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt caatgatctc
    47881 tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg catagttatg
    47941 atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca tcaagtattt
    48001 gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat tcaacagttt
    48061 cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca actgattctt
    48121 attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag gcatgtcatt
    48181 catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt tcgtgtcttc
    48241 atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg gcggagcgcc
    48301 ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag gattttttgt
    48361 catctggtaa tttttataaa taccatatag accgacacct tgtggccaat tccacactgt
    48421 ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac cattatcgtc
    48481 tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta attctttagg
    48541 tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc tttcatttta
    48601 tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa caaaaactgt
    48661 ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg aaattaaaaa
    48721 aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt atcttttttc
    48781 tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca attcagagat
    48841 caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt cagccatacg
    48901 gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac tcatgttgat
    48961 gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta attcctctgt
    49021 tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat ctgcacggaa
    49081 atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat agcggataat
    49141 atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc cgccgatttt
    49201 atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac cagccattgt
    49261 agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg atttttctaa
    49321 atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat ctgaagccat
    49381 gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat ctgctgtgac
    49441 agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc
    49501 ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt
    49561 tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt tttctggttt
    49621 gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac caaagaaaga
    49681 tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg cagaaccgcc
    49741 tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac
    49801 ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca catcattttt
    49861 acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca taccagggtc
    49921 acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata gagagttctt
    49981 ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc gtttcgctac
    50041 agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt tcttaccata
    50101 agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac cgatgccgcc
    50161 ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac cgaatttgac
    50221 atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc caagattcaa
    50281 cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca tggcaagaac
    50341 tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca attcagcaat
    50401 cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt ccatcgcaga
    50461 atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg catcttctaa
    50521 tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt ctgcttttac
    50581 tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa tcattccatt
    50641 atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc
    50701 aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg tagagtaagc
    50761 agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg gatggttcat
    50821 tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat tttcaggagc
    50881 tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag atttttgggc
    50941 acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca ttgatttgaa
    51001 aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac cgactggttc
    51061 agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg ttttatcatg
    51121 tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt tatcttcgta
    51181 aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt gatccaaagc
    51241 tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc tttccatttc
    51301 tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta cgtcaattgt
    51361 cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg gtcatggtta
    51421 tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta aacacttttt
    51481 tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag gtgtttgtat
    51541 agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta ttcaatattg
    51601 agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag gtaggacatc
    51661 tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc ttctcttctg
    51721 tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct ggttcttctg
    51781 ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg accgttttga
    51841 ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact gtccgtttgt
    51901 cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact gaatcacctg
    51961 gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca
    52021 taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat gttcccattt
    52081 ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat gctgataaca
    52141 ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg ttttctttat
    52201 tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca tccatgattg
    52261 cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa gaagtcaaac
    52321 gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt gtatagtttt
    52381 tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca tatttcgcat
    52441 ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg attcgagtag
    52501 gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaac gaatcagcag
    52561 caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta gtgaattcta
    52621 atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt gagtaaccag
    52681 ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca aaccctgctc
    52741 cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc aatcctcgtt
    52801 ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt tcatcgaatg
    52861 gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg atgttgattg
    52921 ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt
    52981 cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct gaatctgtta
    53041 aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct actaagtctt
    53101 caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca cccatttctt
    53161 tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc atgaacattg
    53221 gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca gtatgttttt
    53281 ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct actttctata
    53341 taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg caagttccct
    53401 cggcttcctt ctactttatt taatattctt tttttatcct ttttttatga ataacaaaaa
    53461 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
    53521 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
    53581 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
    53641 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
    53701 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
    53761 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
    53821 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
    53881 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
    53941 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
    54001 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
    54061 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
    54121 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
    54181 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
    54241 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
    54301 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
    54361 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
    54421 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
    54481 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
    54541 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
    54601 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
    54661 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
    54721 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
    54781 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
    54841 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
    54901 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
    54961 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
    55021 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
    55081 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
    55141 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
    55201 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
    55261 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
    55321 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
    55381 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
    55441 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
    55501 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
    55561 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
    55621 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
    55681 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
    55741 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
    55801 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
    55861 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
    55921 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
    55981 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
    56041 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
    56101 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
    56161 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
    56221 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
    56281 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
    56341 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
    56401 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
    56461 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
    56521 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
    56581 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
    56641 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
    56701 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
    56761 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
    56821 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
    56881 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
    56941 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
    57001 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
    57061 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
    57121 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
    57181 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
    57241 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
    57301 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
    57361 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
    57421 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
    57481 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
    57541 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggctttttc
    57601 tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc
    57661 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
    57721 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
    57781 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
    57841 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
    57901 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
    57961 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
    58021 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
    58081 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
    58141 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
    58201 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
    58261 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
    58321 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
    58381 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
    58441 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
    58501 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
    58561 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
    58621 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
    58681 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
    58741 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
    58801 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
    58861 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
    58921 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
    58981 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
    59041 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
    59101 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
    59161 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
    59221 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
    59281 ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg
    59341 aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat
    59401 tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta
    59461 acccatgaca tggatgaagc g
//
LOCUS       DAYYHB010000006        57254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000006 DAYYHB010000000
VERSION     DAYYHB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.006"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..419
                     /locus_tag="ACHA6B_000331"
     CDS             189..419
                     /locus_tag="ACHA6B_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8947937.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="ACHA6B_000332"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="ACHA6B_000332"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8947938.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIIMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="ACHA6B_000333"
     CDS             1830..2006
                     /locus_tag="ACHA6B_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8947939.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="ACHA6B_000334"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="ACHA6B_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8947940.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="ACHA6B_000335"
     CDS             2622..3089
                     /locus_tag="ACHA6B_000335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8947941.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="ACHA6B_000336"
     CDS             3174..4058
                     /locus_tag="ACHA6B_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8947942.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="ACHA6B_000337"
     CDS             4172..4408
                     /locus_tag="ACHA6B_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8947943.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="ACHA6B_000338"
     tRNA            4496..4576
                     /locus_tag="ACHA6B_000338"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="ACHA6B_000339"
     tRNA            4583..4654
                     /locus_tag="ACHA6B_000339"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="ACHA6B_000340"
     CDS             4779..4949
                     /locus_tag="ACHA6B_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947944.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="ACHA6B_000341"
     CDS             5169..5876
                     /locus_tag="ACHA6B_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8947945.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="ACHA6B_000342"
     CDS             complement(5935..6579)
                     /locus_tag="ACHA6B_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8947946.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="ACHA6B_000343"
     CDS             6705..6992
                     /locus_tag="ACHA6B_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8947947.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="ACHA6B_000344"
     CDS             7100..7420
                     /locus_tag="ACHA6B_000344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8947948.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="ACHA6B_000345"
     CDS             7501..8814
                     /locus_tag="ACHA6B_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8947949.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="ACHA6B_000346"
     CDS             8811..9530
                     /locus_tag="ACHA6B_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8947950.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACHA6B_000347"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACHA6B_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8947951.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACHA6B_000348"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACHA6B_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8947952.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACHA6B_000349"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACHA6B_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8947953.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHA6B_000350"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHA6B_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8947954.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHA6B_000351"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHA6B_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8947955.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACHA6B_000352"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACHA6B_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8947956.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="ACHA6B_000353"
     CDS             17797..18654
                     /locus_tag="ACHA6B_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8947957.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="ACHA6B_000354"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="ACHA6B_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8947958.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="ACHA6B_000355"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="ACHA6B_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8947959.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="ACHA6B_000356"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="ACHA6B_000356"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8947960.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /gene="pstB"
                     /locus_tag="ACHA6B_000357"
     CDS             21410..22168
                     /gene="pstB"
                     /locus_tag="ACHA6B_000357"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8947961.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="ACHA6B_000358"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="ACHA6B_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8947962.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23434..24126
                     /locus_tag="ACHA6B_000359"
     CDS             23434..24126
                     /locus_tag="ACHA6B_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8947963.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24123..24890
                     /locus_tag="ACHA6B_000360"
     CDS             24123..24890
                     /locus_tag="ACHA6B_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8947964.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="ACHA6B_000361"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="ACHA6B_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8947965.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26855..27175
                     /locus_tag="ACHA6B_000362"
     CDS             26855..27175
                     /locus_tag="ACHA6B_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8947966.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27175..27525
                     /locus_tag="ACHA6B_000363"
     CDS             27175..27525
                     /locus_tag="ACHA6B_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8947967.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /gene="hprK"
                     /locus_tag="ACHA6B_000364"
     CDS             27692..28630
                     /gene="hprK"
                     /locus_tag="ACHA6B_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8947968.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /gene="lgt"
                     /locus_tag="ACHA6B_000365"
     CDS             28645..29475
                     /gene="lgt"
                     /locus_tag="ACHA6B_000365"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8947969.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFVEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="ACHA6B_000366"
     CDS             29519..30544
                     /locus_tag="ACHA6B_000366"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8947970.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="ACHA6B_000367"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="ACHA6B_000367"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8947971.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="ACHA6B_000368"
     CDS             31622..32062
                     /locus_tag="ACHA6B_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8947972.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="ACHA6B_000369"
     CDS             32065..32667
                     /locus_tag="ACHA6B_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8947973.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="ACHA6B_000370"
     CDS             complement(32726..33832)
                     /locus_tag="ACHA6B_000370"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8947974.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="ACHA6B_000371"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="ACHA6B_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8947975.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="ACHA6B_000372"
     CDS             complement(35380..37725)
                     /locus_tag="ACHA6B_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8947976.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="ACHA6B_000373"
     CDS             38004..38906
                     /locus_tag="ACHA6B_000373"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8947977.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="ACHA6B_000374"
     CDS             39119..40894
                     /locus_tag="ACHA6B_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8947978.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="ACHA6B_000375"
     CDS             complement(40960..42546)
                     /locus_tag="ACHA6B_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8947979.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            42797..43021
                     /locus_tag="ACHA6B_000376"
     CDS             42797..43021
                     /locus_tag="ACHA6B_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8947980.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43162..44913
                     /locus_tag="ACHA6B_000377"
     CDS             43162..44913
                     /locus_tag="ACHA6B_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8947981.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44913..46697
                     /locus_tag="ACHA6B_000378"
     CDS             44913..46697
                     /locus_tag="ACHA6B_000378"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8947982.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47023..47874
                     /gene="licT"
                     /locus_tag="ACHA6B_000379"
     CDS             47023..47874
                     /gene="licT"
                     /locus_tag="ACHA6B_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8947983.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47971..49851
                     /locus_tag="ACHA6B_000380"
     CDS             47971..49851
                     /locus_tag="ACHA6B_000380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8947984.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49891..50793)
                     /locus_tag="ACHA6B_000381"
     CDS             complement(49891..50793)
                     /locus_tag="ACHA6B_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8947985.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51024..52427
                     /locus_tag="ACHA6B_000382"
     CDS             51024..52427
                     /locus_tag="ACHA6B_000382"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8947986.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52427..53860
                     /locus_tag="ACHA6B_000383"
     CDS             52427..53860
                     /locus_tag="ACHA6B_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8947987.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54005..54931
                     /gene="trxB"
                     /locus_tag="ACHA6B_000384"
     CDS             54005..54931
                     /gene="trxB"
                     /locus_tag="ACHA6B_000384"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8947988.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55145..56872
                     /locus_tag="ACHA6B_000385"
     CDS             55145..56872
                     /locus_tag="ACHA6B_000385"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8947989.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56887..57237
                     /locus_tag="ACHA6B_000386"
     CDS             56887..57237
                     /locus_tag="ACHA6B_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8947990.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        18566 a         9958 c        12366 g        16364 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat cataatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtc cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttgttgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgacatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc ataattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agtacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccgcggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaatg ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtc atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gggtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaat
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatcct
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagcc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agatcgtggc gtgaaagtac cggaaggatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc ggtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg agcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ttcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt cgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatctcttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttggtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttccaggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttctacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggattgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcacct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaacgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaggagtt aaaacgtgag aaaatgatct tttttataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccgacacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta
    39481 tcttctttta agtttctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttactagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcagtggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aaccatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctacca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    40801 tggcgcaggc gtgccgttaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccat
    40981 ttcctgcaaa cgctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggagg aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggga tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca aagcggtatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca agataccatt
    41581 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttgaac
    41641 agaaccggtt ttgaagacag cgacacccat aatcaatagc agcgcattga ctagaaaagc
    41701 catcgacaat tggatattag agtcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgtcttgg tgatcgatct tccgagtttg tgaaactgct gaatgtagat ataagttatg
    41821 tggcatgact gttgcgccaa taatccctaa tgctccagtt agcggagttt cgccaccaat
    41881 gacaggtgag tcggagaatg tatggctgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaatcaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacccaatg tcttacttgt gcgtgctcgg attgcttggg ccaaatccat
    42241 ttgggtcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga
    42361 ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatccact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttgt tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaagattc ggtatgccta attttttttc gtggcgtgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa agacaagcac agacaatcct aggctcttga
    43141 taaagacaaa ggaggagtaa aatgtcgatt tttagaaagt taggctggtt ttttcgtcag
    43201 gaaaagaaac attatttaat tggggttttc tcgctattcg cagtggcctt ggtacagtta
    43261 gttcctccaa aagtgattgg gatcatcatt gatgagattg ccgataaaaa tttatcagta
    43321 aagatcattt tcgggtggat cttcctattg attatcgctg cttttttaca atatcttttt
    43381 cgttatatct ggcggacgaa tatttgggga agtgcagctc ggttggagaa agaacttcgg
    43441 cagcagttat tcaaacattt tacagaaatg gacaatctct tttaccaaaa atatcggact
    43501 ggggatttga tggctcatgc aacgaatgat ttgaatgcga tccagaatgt agcaggagct
    43561 ggtatcttga cctttgcaga ttcagtgatt acgggcggaa caacgatcat tgcgatgatt
    43621 ttgtttattg attggcgctt aacactgatt gcgctgattc ctctgccttt gctggccgtc
    43681 acttcacgag tgctggggtc gaaactccat gatgcttttc gtgattcgca agaagctttt
    43741 tctagtatca atgataagac acaagaaagt atcactggga tcaaagtcat taaaacattt
    43801 ggacaagaaa aagaagatct agcggatttt gaagaaaaga ttgaagatgc aattattaaa
    43861 aacaaacgag tcaatttttt agactcctta tttgatccat tcattacctt gattatcggt
    43921 ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg tcttggaagg cacgatctcg
    43981 atcgggcaga tggtctcatt tattagttat atcggtatgc ttgtttggcc gatgtttgcg
    44041 attggtcgat tgttcaacgt attggaaaga ggaaatgctt cttatgatcg cgtcaatgaa
    44101 cttttgcatg aaaaaacgca tatcgtggaa caactgggtg cagtgcagac cccggcaaaa
    44161 ggcgagctga atgtagctat ttcctctttt aattatccga aagaagaatc accagcatta
    44221 gaacaaatca agtttgcaat ccaagaagga gaaacattag gtattgttgg gaaaactggt
    44281 gcgggaaaaa cgacgatcat caaattactg atgcgtgaat acgatgatta tcaagggaaa
    44341 atccaatttg gcggacgttc gatcaaagaa tattcattga atgctttatt aggatcaatc
    44401 ggctatgtgc ctcaggatca cttcttgttt tcgatgacca ttcgagataa tatccgtttt
    44461 tctaatccaa aactttcaga agaagaagta gagcatgcgg cttctttagc atttatcaac
    44521 aatgaaatca aggaattacc aaaaggttat gataccttag ttggagaacg tggagtctct
    44581 ttatctggcg gacagaaaca aaggatttcg attgctcgag cattgatcaa aaatcctgaa
    44641 ttattaatat tagatgatgc actttctgca gtagatgcca aaacagaaga agctatcttg
    44701 cataatttaa aagaaataag aaaagagaaa acaactatca tttcagctca ccgtttaagt
    44761 agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag ggaaaatcat tgaacgagga
    44821 acccatcatg agctgcttgc actaggcggc tggtatgaac aaatgtggga gaaacagcaa
    44881 ttagaagcga agatagaagg aggcgaggcc tgatggatga aaattatcaa tcggaatgga
    44941 caaaatcgat tccacttaaa gaacagaaaa agatcatcaa acgactgatg aagttcgcca
    45001 aaccttttag aagaacgttc atcgttgcta tcttatttgc gtttgctctg tctgtgatca
    45061 atgtcctatt gccaaggatc atccaaactt ttatggatga tcatctggct aaacagtcgg
    45121 ctacgacaca agttattttg ttttttgctg gtatgtactt atttggagtc atagtaaaaa
    45181 gtatcatttg gtttttccaa tggtatttgt attccatggc ttcattgaag acctatcagt
    45241 atattagggt aaaattattt gaaaagctgc atacactagg tatgcgttat tttgatcaga
    45301 caccggcagg atcaaccgtt tctcgagtga cgaatgacac agaaacgcta tttgaatttt
    45361 ggtacgtctt tttgatggta ttgactggga tctttgcagt cgtttcttca ttcattgcca
    45421 tgtttcaaat caatggagaa attgccttgt ggactttggt gttcttgcca attttaggaa
    45481 tagtcatctg gtattaccag aaatttagtt ctcgtattta ccggaatatg cgggaaaaat
    45541 taagtcagct gaatacgaaa ttgaatgaat cgatttccgg catgcgtatt atccagcagt
    45601 ttcgccaaga agctcgttta gctaaagaat ttgaacaagt gaacaatgag tatttagaca
    45661 cgcgttatgc gatgatcaaa acaaattcga ttctgttaag tccgatcatc aattttctat
    45721 atgcattggc gattgcgttg gtgttgactt tgtttgggat cgatgcgttg cattctcccg
    45781 tagaagttgg actgatctat gcatttacga cctatgtcca gggatttttt aatccaatgt
    45841 ctcaaatgat ggactttttg agcatcttca cagatgggat ggttgctggt agtcgtatct
    45901 tgaagattat ggatacagaa gaatatgcac cacaacaaaa ccctggagca gatcaaatga
    45961 ttacttcagg taagattgag tttcgcaatg taagcttttc ttatgatgga gagcacaatg
    46021 tattaaacaa tatctctttt gttgctaatc cgggagagac cgttgcatta gtgggacaca
    46081 ccggaagtgg gaaaagctcg atcatcaatg tgttgatgcg gttttacgaa ttttatgaag
    46141 gtcagatatt gattgatgat catgacattc gagaatatcc aatggaagag ttacgtaaga
    46201 aaatggggct ggtcttgcaa gatgctttct tgttttatgg ggatatcgca ggaaatattc
    46261 ggatgatgaa tccagctatc acagacgaac agatacgcca tgcggctgag tttgttcaag
    46321 caaacaaatt catcgatgaa ttaccaaaag gatatcactc aaaagtgatc gaacatggtg
    46381 ccagctattc tagtggacaa cgacagctga tcacttttgc gcgaacaatc gtgactgatc
    46441 ctaaaattct agtattggat gaagcaacag caaatatcga tactgaaaca gaaggattga
    46501 tccaagaagg attggcgaaa atgcgacaag gacggacaac gattgcgatt gcccatcggc
    46561 tgtcaacgat tcgagatgca gaattgattt tagttttaga caaaggtcat atagtagaac
    46621 gcgggaatca tgaaacactt ctagcgcaaa atggtttgta tgcagatatg tacaaactgc
    46681 aaagcagtgg tatataaaag atgttgtgac agtagcattt tgacctaagc actagagtga
    46741 aaataaaaca tagcttccca tatttttcat ctgtgagagt tagtgtcgta gttaccagat
    46801 ttaaaaaaga cgacgtcagc tagtcagaag tgcctaatag cccctaagaa agcgttgaca
    46861 aaatcgtttg ctttcgataa tataaagaaa acgatacaac ttggatagtg attgcctgat
    46921 gcaagctaaa cctaatgagt ctcccatggt ttcatggacg gctttttggg tttttctttt
    46981 tgttttggat acagccctat atatcatgga aaggagcaaa acatgctgat tgagaaagta
    47041 ctgaataaca acgtcgtaat aacaagggat gaaagagatc gggaaatgat tgtgatggga
    47101 aaaggattgg cattcaaaaa gaaggtgggt gacccgattc ttgattcatg catcgacaaa
    47161 aaatttcagt tgacaaatga cggactttca caacagttcc aagaattggc acaagcgatt
    47221 cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga ggctctgcaa
    47281 gtccatttaa atgattctgt gtatatttca ttgacggatc acttgtatac agctatcgaa
    47341 cgtcaaaaaa tggggatctt tgtgccaaat gtattgctgt atgatatcaa acaactgttt
    47401 cctaaagagt atgccattgg gaaaaaaaca gtgaagaaaa tctcggataa ataccacatc
    47461 aagttatcag aagacgaagc cggatttatc gcattgcatt tagtcaatgc acaaggcatt
    47521 caaagccagc aagacaatac ctacctgttt accacaacaa tacaggatat tttaaatatc
    47581 gtccgctatt actataagat tacatttgat gaagattcag tccacttttt tcgattcgtc
    47641 acacacctgc gtttttttct gacacgtgtt ctagatggag cgaaatcaga agatggagaa
    47701 gtttctgagg aattgttcca agttgttcaa aaaaaatata aggatacgtt tggttgcgtt
    47761 gagaaaatag cagacttttt aaccggggct ttcgactacc agatgtccac ggatgaaaaa
    47821 atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa ataaataaaa
    47881 aaggaggaca aattaacaag aacgtatgaa aataagggta gtgacattca gttggctaaa
    47941 ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac
    48001 atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga
    48061 cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa
    48121 gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg
    48181 ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagt agaagaagag
    48241 gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag
    48301 ccttttttag gtgctttagc tgcagcggga atggtcaaag gattaaatgc tttgttcttg
    48361 tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat
    48421 gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg
    48481 aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca
    48541 gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg
    48601 gcttatgaaa catttttggg tatcccatgg gtgggcagca gttataccag tagcgtcgta
    48661 cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt
    48721 cctgcagtcg tccagacctt tttggttcct ttttctgtgt tattaatcgc acttcctgct
    48781 ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc
    48841 caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa
    48901 gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg
    48961 caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca
    49021 gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca
    49081 gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg
    49141 aagaagaagc catttattta ctcgatgatt ggtggtgcca tttcaggaat ctatttgatg
    49201 gcatctggaa caatcagcta cacaatgggc ggattaggga tttttggggt ggtaaactac
    49261 atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg
    49321 gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct
    49381 atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca
    49441 ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttgatc
    49501 taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagtcatgac cctgttcccg
    49561 acgaaacatg caattggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga
    49621 ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga
    49681 gtgaaacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac
    49741 agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac
    49801 caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg
    49861 tgaagaatgg aataggctta actattcttt ttattccact tctttatttt caaaattgaa
    49921 aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat
    49981 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
    50041 aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg
    50101 tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt
    50161 caaagctaaa attggaacat gattttgttt caatatttct gcggtacgaa gaatcacttt
    50221 attttcaccc gtataagaaa tcaggatccc gcacatatcg ctcgtgcaat tgtacgcttc
    50281 atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt
    50341 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt
    50401 tgcctggagc agtaaatact tggctttttg cagttggtca tgatgaatga gagaaagtgt
    50461 atcttgaatc gtcatctgtt ctaaagaagc cattttattt gcgatcgtca taataccgtc
    50521 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
    50581 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
    50641 acggatgagt gtagacgggt ggacatagca tcgctcagcg atctcttgga tggtcatttc
    50701 gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa
    50761 ggagatttct ttgatttttt cacgtaataa catgtagtta ccttcttctt atttttttac
    50821 gtaaattata acttttttga aaatgttttg taaacggatt tatactatat aacttaaaat
    50881 agactttttt tgacaagata tttacaaaaa tccactagaa aacagtaact tttgtaaatg
    50941 ttactggaaa gaaacggatt tctattgttt gttttgttta caaaaaagat aatgaaagtg
    51001 taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg attggtggag
    51061 gcagcagtta cacccctgag ttgatggaag gatttatcaa aagatatgag gaattaccga
    51121 tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag atcgttagtc
    51181 agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt caaaatctat gcaactctgg
    51241 atcgggaaga agcattgaat gatgcggatt ttgttacgac tcagtttcgt gtcggtcaat
    51301 tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt gggcaagaaa
    51361 caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtggtc ttggcaatcg
    51421 ttgaagacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg aacccaagtg
    51481 ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt ggattatgca
    51541 atgtgccagt aatggcgatg atgacagaac cagcaatgct tggcaagaaa aaagaagagc
    51601 tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca gataataaag
    51661 gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagacaat ggattgccga
    51721 aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaat atgatccctt
    51781 gtggctacca ccgctattac taccgtgaag aagaaatgct ggaacatgct ttagaagaat
    51841 atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag ctatttgaat
    51901 tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga ggtggcgctt
    51961 attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa gaaacacaga
    52021 tcgtggtatc tacaaagaat gatggagctg ttcctgacct gccggcagat tgtgtagtag
    52081 aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt tctcttcctg
    52141 ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg atcgaagcag
    52201 ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatccg cttatacgat
    52261 caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag cagtatttac
    52321 cgcaattcaa ggaaacaatc agccgattaa aagaagaagg aatcgaagtt caagatgaac
    52381 tggcaagaga attatcaggt aatacacata taatggagga agtataatgg gattcagaaa
    52441 agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcggtt atgatgaagg
    52501 tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt ttcctgttat
    52561 caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag caaaaattgc
    52621 aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa tggggttcaa
    52681 gacttatcga atgtccatcg cctggagtcg tatttttcca atgggggatg aaaaagagcc
    52741 gaatgaagct ggcttgaaat tttatgagga tgtctttaaa gaatgccaaa agcacggaat
    52801 cgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca aagaatacgg
    52861 tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag tcatcttcaa
    52921 tcgatataaa ggacttgtca aatactggct gacattcaat gagatcaata tgatccttca
    52981 tgcaccattc atgggagcag gactgtactt tgaagaagga gaaaacgaag aacaagtcaa
    53041 atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga ttgctcatga
    53101 agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt atccatacac
    53161 ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt atttcttcat
    53221 tgatgttcag tcacgaggag aatacccagc ttatgcattg aaagaaatgg aacgaaatgg
    53281 cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata ctgttgattt
    53341 tatctctttt tcttattatt cttcccgagt ttcgactacc gacaacgaac tcttggaaca
    53401 gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa gtgaatgggg
    53461 gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg atcgttatca
    53521 aaaaccatta tttattgtag aaaacggctt aggtgcagtc gatacgccag atgagaatgg
    53581 ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg cgatgaagga
    53641 tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat gtattgattt
    53701 agtatcagct ggtaccggtg agatgaagaa aagatacgga ttcatttatg tcgatcgtga
    53761 taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact ggtataaaaa
    53821 agtaattgat tcaaatggag aaaatctttc gaacgattga tcaatgattt ataaaagtct
    53881 attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc aagcagaaca
    53941 atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa aggagaatct
    54001 acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg ctgctttata
    54061 tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg gcggccaaat
    54121 gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg gccccgatct
    54181 agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt atggaatcgt
    54241 tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata aaagttacga
    54301 aggaaaaaca gtcattatcg caacaggctg tatccaccgt aaattaggcg tacctggcga
    54361 ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg cgtttttccg
    54421 taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag ccatctattt
    54481 gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac gtgcacaaaa
    54541 gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg atacagtcgt
    54601 tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta atgtcaaaac
    54661 cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac tggatccatt
    54721 aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc caacagacaa
    54781 cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg aaaaagattt
    54841 gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag tgtataaata
    54901 cttggaatct ttagaagaaa caactgcata agtttaaaaa gagtagaaga acgggttcaa
    54961 ttgttctttt actctttttt ttcgcgtgaa atttactgaa tctgatttat gcgaacacgt
    55021 ccaacacctt ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt
    55081 tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat
    55141 tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa
    55201 cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta
    55261 tgcaccgttg gaattcggta ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg
    55321 aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca tggatacaca
    55381 aggtgaagaa gcaaaacgcc gaggtgtcgc tattgcttat gactcacgcc atcagtcacc
    55441 agaatttgca atggaagcag ccaaaacact ggcacatcac aatatcccgt cttatgtatt
    55501 cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga agacgtttgc
    55561 gggaatcatg gtaactgcat ctcacaaccc agcggcttac aatggatata aagtctacgg
    55621 agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga
    55681 agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac atagcgggtt
    55741 gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca aagcagtgac
    55801 gattgatcgt gaattggtag aaacaatggg gaaagatttg aaacttgtgt acacgccact
    55861 tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt
    55921 cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca aatcgccaaa
    55981 tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag aaggagctga
    56041 cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc gtttgccaga
    56101 tggttcttat gaagtattga ctgggaacca aatcggggca ttgtttgctc aatatatttt
    56161 ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga aatcaatcgt
    56221 ttccagcgaa ttgcctacag caattgcgga acactacggt gctaccatgt tcaatgtgtt
    56281 gacagggttt aaattcattg ctgaaaaaat ccaacaattc gaagaagacc attcacatac
    56341 ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg ttcgcgataa
    56401 agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata aaaaacaagg
    56461 caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt ttgcagaaaa
    56521 aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga
    56581 aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc aaacagaaga
    56641 ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa caacaccgcc
    56701 atccgacgta ttgaaatata cattggaaga aaatggctgg attgctatcc gcccttcagg
    56761 aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag aagacgcagc
    56821 cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat aaaaaacttg
    56881 agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt ggaggtgaat
    56941 ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt caaaatgttg
    57001 agtgatccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca aaatgtgaca
    57061 gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact acgtttatta
    57121 agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta tagtcttgat
    57181 gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca tcaataacga
    57241 taggtgcaaa taga
//
LOCUS       DAYYHB010000007        56362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000007 DAYYHB010000000
VERSION     DAYYHB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.007"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2035
                     /gene="tdc"
                     /locus_tag="ACHA6B_000387"
     CDS             158..2035
                     /gene="tdc"
                     /locus_tag="ACHA6B_000387"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8947991.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2278..3699
                     /gene="tyrP"
                     /locus_tag="ACHA6B_000388"
     CDS             2278..3699
                     /gene="tyrP"
                     /locus_tag="ACHA6B_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8947992.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3808..5175
                     /gene="nhaC"
                     /locus_tag="ACHA6B_000389"
     CDS             3808..5175
                     /gene="nhaC"
                     /locus_tag="ACHA6B_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8947993.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5456..6475)
                     /locus_tag="ACHA6B_000390"
     CDS             complement(5456..6475)
                     /locus_tag="ACHA6B_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8947994.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6497..7504)
                     /locus_tag="ACHA6B_000391"
     CDS             complement(6497..7504)
                     /locus_tag="ACHA6B_000391"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8947995.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7497..8162)
                     /gene="pgmB"
                     /locus_tag="ACHA6B_000392"
     CDS             complement(7497..8162)
                     /gene="pgmB"
                     /locus_tag="ACHA6B_000392"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8947996.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(8155..10449)
                     /locus_tag="ACHA6B_000393"
     CDS             complement(8155..10449)
                     /locus_tag="ACHA6B_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8947997.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10709..12874
                     /locus_tag="ACHA6B_000394"
     CDS             10709..12874
                     /locus_tag="ACHA6B_000394"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8947998.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13178..14011
                     /locus_tag="ACHA6B_000395"
     CDS             13178..14011
                     /locus_tag="ACHA6B_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8947999.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14113..15813)
                     /locus_tag="ACHA6B_000396"
     CDS             complement(14113..15813)
                     /locus_tag="ACHA6B_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948000.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            16320..17480
                     /locus_tag="ACHA6B_000397"
     CDS             16320..17480
                     /locus_tag="ACHA6B_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8948001.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17496..17939
                     /locus_tag="ACHA6B_000398"
     CDS             17496..17939
                     /locus_tag="ACHA6B_000398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8948002.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18422..18952)
                     /locus_tag="ACHA6B_000399"
     CDS             complement(18422..18952)
                     /locus_tag="ACHA6B_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8948003.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            19057..19608
                     /locus_tag="ACHA6B_000400"
     CDS             19057..19608
                     /locus_tag="ACHA6B_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8948004.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19761..20234)
                     /locus_tag="ACHA6B_000401"
     CDS             complement(19761..20234)
                     /locus_tag="ACHA6B_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8948005.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20372..21844
                     /locus_tag="ACHA6B_000402"
     CDS             20372..21844
                     /locus_tag="ACHA6B_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8948006.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21841..22551
                     /locus_tag="ACHA6B_000403"
     CDS             21841..22551
                     /locus_tag="ACHA6B_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8948007.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22903..23187
                     /locus_tag="ACHA6B_000404"
     CDS             22903..23187
                     /locus_tag="ACHA6B_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8948008.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23504..23887)
                     /locus_tag="ACHA6B_000405"
     CDS             complement(23504..23887)
                     /locus_tag="ACHA6B_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8948009.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(23889..24473)
                     /locus_tag="ACHA6B_000406"
     CDS             complement(23889..24473)
                     /locus_tag="ACHA6B_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8948010.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(24520..25995)
                     /locus_tag="ACHA6B_000407"
     CDS             complement(24520..25995)
                     /locus_tag="ACHA6B_000407"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8948011.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26176..26487
                     /locus_tag="ACHA6B_000408"
     CDS             26176..26487
                     /locus_tag="ACHA6B_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948012.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26602..28938)
                     /locus_tag="ACHA6B_000409"
     CDS             complement(26602..28938)
                     /locus_tag="ACHA6B_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8948013.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            29080..29748
                     /locus_tag="ACHA6B_000410"
     CDS             29080..29748
                     /locus_tag="ACHA6B_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8948014.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29751..30794
                     /locus_tag="ACHA6B_000411"
     CDS             29751..30794
                     /locus_tag="ACHA6B_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8948015.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(31031..31912)
                     /locus_tag="ACHA6B_000412"
     CDS             complement(31031..31912)
                     /locus_tag="ACHA6B_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8948016.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            32071..32799
                     /locus_tag="ACHA6B_000413"
     CDS             32071..32799
                     /locus_tag="ACHA6B_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8948017.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32955..33272
                     /locus_tag="ACHA6B_000414"
     CDS             32955..33272
                     /locus_tag="ACHA6B_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8948018.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33265..33873
                     /locus_tag="ACHA6B_000415"
     CDS             33265..33873
                     /locus_tag="ACHA6B_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8948019.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33928..34719
                     /locus_tag="ACHA6B_000416"
     CDS             33928..34719
                     /locus_tag="ACHA6B_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8948020.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            34857..35345
                     /locus_tag="ACHA6B_000417"
     CDS             34857..35345
                     /locus_tag="ACHA6B_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8948021.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            35396..36268
                     /locus_tag="ACHA6B_000418"
     CDS             35396..36268
                     /locus_tag="ACHA6B_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8948022.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36444..36806
                     /locus_tag="ACHA6B_000419"
     CDS             36444..36806
                     /locus_tag="ACHA6B_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8948023.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36815..37567
                     /locus_tag="ACHA6B_000420"
     CDS             36815..37567
                     /locus_tag="ACHA6B_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948024.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37581..39383
                     /locus_tag="ACHA6B_000421"
     CDS             37581..39383
                     /locus_tag="ACHA6B_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948025.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39660..40700
                     /locus_tag="ACHA6B_000422"
     CDS             39660..40700
                     /locus_tag="ACHA6B_000422"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8948026.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(40752..42896)
                     /locus_tag="ACHA6B_000423"
     CDS             complement(40752..42896)
                     /locus_tag="ACHA6B_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8948027.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(43253..44626)
                     /locus_tag="ACHA6B_000424"
     CDS             complement(43253..44626)
                     /locus_tag="ACHA6B_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8948028.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(44626..45021)
                     /gene="rbsD"
                     /locus_tag="ACHA6B_000425"
     CDS             complement(44626..45021)
                     /gene="rbsD"
                     /locus_tag="ACHA6B_000425"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8948029.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(45049..45966)
                     /gene="rbsK"
                     /locus_tag="ACHA6B_000426"
     CDS             complement(45049..45966)
                     /gene="rbsK"
                     /locus_tag="ACHA6B_000426"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8948030.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(46074..47087)
                     /locus_tag="ACHA6B_000427"
     CDS             complement(46074..47087)
                     /locus_tag="ACHA6B_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8948031.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(47168..47767)
                     /locus_tag="ACHA6B_000428"
     CDS             complement(47168..47767)
                     /locus_tag="ACHA6B_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8948032.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            47953..48654
                     /locus_tag="ACHA6B_000429"
     CDS             47953..48654
                     /locus_tag="ACHA6B_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948033.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            48664..51375
                     /locus_tag="ACHA6B_000430"
     CDS             48664..51375
                     /locus_tag="ACHA6B_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8948034.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            51826..53454
                     /gene="treC"
                     /locus_tag="ACHA6B_000431"
     CDS             51826..53454
                     /gene="treC"
                     /locus_tag="ACHA6B_000431"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8948035.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(53514..54695)
                     /locus_tag="ACHA6B_000432"
     CDS             complement(53514..54695)
                     /locus_tag="ACHA6B_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8948036.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            54947..>56362
                     /locus_tag="ACHA6B_000433"
     CDS             54947..>56362
                     /locus_tag="ACHA6B_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8948037.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYA"
BASE COUNT        17655 a        10130 c        11551 g        17026 t
ORIGIN      
        1 gtttttttta attatttcaa aagtgtgaac aattcggata attgatacat attaaaatat
       61 gattaaaaat caggccaaaa cttagtttac agaatagaag aacaggtgta atgtagggct
      121 cgacaaaatt taaatatttt tttaggaggg tttcactatg agtgaatcat tgtcgaaaga
      181 tctaaaccta aatgccctat tcattgggga caaagctgag aacggccaaa tctataaagc
      241 tttgttgaat gaattggtgg atgaacattt aggctggcgt caaaactaca tgcctcaaga
      301 catgcctatt attacgccag aagaaaaaag cagtgctagt tttgaacaca ctgtgaataa
      361 aacaaaggat gttctatcag aaatttcagc gcggatgcgt acccattccg ttccttggca
      421 taatgccgga cgttactggg gtcatatgaa ctctgaaaca ttgatgccat ctttactagc
      481 gtacaatttt gctatgctat ggaatggtaa taacgttgcc tatgaatctt ctcctgcaac
      541 aagccaaatg gaagaagaag taggaatgga atttgcaaaa ttaatgagct ataaagatgg
      601 ctggggccac atcgttgctg atggttcttt agcaaactta gaaggacttt ggtatgcccg
      661 caacatcaaa tcattgccgc ttgcaatgaa agaagtgaca cctgaactag ttgctggaaa
      721 aagcgattgg gaattaatga acttgtcaac agaagaaatc atgaatctgt tagacagcgt
      781 cccagaaaaa atcgacgaaa tcaaagctca ctcagcacgt agcggaaaac atttagaaaa
      841 attaggaaaa tggttggttc cacaaactaa acactattca tggctaaaag ctgccgacat
      901 catcggtatc ggtttagacc aagtgattcc agtgccagtt gaccacaact atcgcatgga
      961 catcaacgaa cttgaaaaaa tcgttcgtgg tctagcagct gaaaaaacac caatcctagg
     1021 tgtagtcggt gttgtaggat caacagaaga aggagcaatc gacggtatcg ataaaatcgt
     1081 tgcattgcgt cgcgtattag aaaaagacgg tatttacttc tacttacacg tagatgctgc
     1141 ttatggcgga tacggccgtg cgattttctt agacgaagac aacaacttca ttccatttga
     1201 agatttaaaa gatgttcatt acaaatacaa tgtctttaca gaaaacaaag attatatctt
     1261 agaagaagta cacagtgcat ataaagcaat cgaagaagca gaatctgtaa cgattgaccc
     1321 acataaaatg ggctatgttc catattctgc cggtggtatc gtcatcaaag atatccgcat
     1381 gagagacgtt atctcttact ttgcgacata tgtatttgaa aaaggcgcag atatcccagc
     1441 attacttggg gcttatatct tggaaggttc aaaagcaggg gcaacagcag ccagcgtttg
     1501 ggcagcacac catgtcttac ctttgaatgt cacaggatat ggtaaattaa tgggtgcatc
     1561 aatcgaaggt gcacatcgtt tctacaactt cttgaatgac ttgtcattta aagtaggaga
     1621 caaagaaatc gaagttcatc cattaactta tccagatttc aatatggtag actatgtatt
     1681 caaagaaaaa ggcaacgatg acttagttgc aatgaataaa ttgaaccatg acgtatatga
     1741 ttattcttca tacgtgaaag gaagcattta cggtaatgag ttcttaactt cacatactga
     1801 ctttgctatc ccagactatg gcaacagccc attacaattt gtgaaccaac taggattctc
     1861 agatgaagaa tggaaccgtg ccggaaaagt aactgtattg cgtgccagcg taatgacacc
     1921 atacatgaac aaagaagaac actttgaaga atatgctgaa aaaatcaaag cagctcttca
     1981 agaaaaattg gaaaaaatct acgcagacca attattggca agcgaagcaa aataagcaag
     2041 gtaatcatgt tagaaaacag cttgaatact aactgtttct gacgcatgtt tggatctgaa
     2101 gagcaagggg agacctaagc tcttcgatcc ctttgttttg aggttgtctt tgaagaaacc
     2161 cttcaagcaa taaactcaaa gaatcccctc aaatattcgc tgaattgttt cgtttattgc
     2221 tgaaactact tcaaagacac cgttatttta tccaaaaaat caaggagagt gtcaacggtg
     2281 agtaaatctg aagtaagtaa aatgggtctt gggaccttta tcggtctgac aatggcactg
     2341 tgtgcaacgg ttcggagtat tcctacgtta gccgcagtcg gttggacatt gattttttat
     2401 tcaatttttg cagttttgtt ttttgccggt ccgatatcaa tggtttccgg tgagctttcc
     2461 acgatgcttc ctcaagaagg cggtcctcag ttatgggtaa aaacagcact gggcagcaaa
     2521 tggggatttg tcgttgcatg gctattatgg gtgcaaatgt tcccaggtat ggtaatggtc
     2581 gcttctactt taggaccatt gttaggaaac acatttggta atgtagaact tggcaataat
     2641 cactggttcg ttttaggttg tattttagtt atctactgga tcatcacgat cttgaacttg
     2701 aaattcgata tggcaaaagt cggcgggaac atcggtgtat ggctaggggt atatatccca
     2761 gtagtcatca tgttcgtttt aggtgttttg gcagccttca aagtcggact agtctcaaac
     2821 ggctatctag gtgacttttc atggtcaaaa gctttccctg acttggaaca tattgattca
     2881 ttgaaatact tagctggtat cacatttatt tttgttggga tcgaaatgag ttctgtttac
     2941 atgcctcgtt tgaaagacgc aacaaaaaat tatacaaaag gcgtatttat cgctttgatc
     3001 ggattagttt tattgaatgt catcaatgcg atgcttgtag caaatgtcgt accagatgga
     3061 aaaatggaat tagcaaatat cacgcagcct atcttgattg actgtcaaat cctaggctta
     3121 ccagaagtga tcggaaatat cttcagcttc atggtattta ttggtgtgct tttacaatta
     3181 tcagcttggg ttactggacc atcaaaaaca atcatccaag tagctcgtga aggattcctt
     3241 ccaccaaaat tcggattcca taaagaaaac aaatacggcg tatctagaaa tgtcgttcta
     3301 acacaatcaa tcgttatttc attatttgct ttgctttatg gtgtaatgga tgacgtaagt
     3361 gctgtattct tgacacttac aaatgctaca actgttattt attgtatcgt gtacattttg
     3421 attgctgttt cattgcttaa aatgcgtaag aaacatccag aattcgaacg tccttaccga
     3481 attggtaaaa acggaaacgg attagcttgg gtagtcagct gcatgctgat cttctctatc
     3541 atcgttgtcg tatttgctac attaggtaca gcgacattga gtgatgcatt attagtagca
     3601 gctatcacag tggtcatgtt cgttattcca ttgatcatca atcacttcaa gaaagattct
     3661 tgggggattg aggtagaaaa aagtttagaa gaaaaataaa aaaataagtc tttcagccga
     3721 cgttttgtcg ctgatttagt aggaggtcct gccaagtgaa tagttttttc atggcagggc
     3781 cttttcattt gaaataggag atcgacgatg aataaaaata tttctttaaa agaaagtatc
     3841 gctgtattga tcgtgttgtt ggcactccta ggagtactga tcatcggatt ccagctctcg
     3901 ccacatatcc cgatccttgc agctttcatg tgtttgttgt tttatggcag atttaaaaag
     3961 ttcacatggg atgagatcca tgacgggatc atcaaaggaa tcactccagg gatcattcca
     4021 atcctgattt ttcttttgat tggtgtctta gttgcttcat ggattttatc tggaaccatc
     4081 cctacgatta tggtttatgg atttaaactt gtttctgtgc gtttcttttt accgacagca
     4141 tttcttgttt gtgcaattgt cggagtgact gtcggcagtt catttacaac gatttcgacg
     4201 attggaattg cttttttggg gatcggtcat ttacttggat tcaatgatgc aatgactaca
     4261 ggcgcagtcg tttccggtgc ctttttaggg aataactgct cgcctttatc agatacgacc
     4321 aacttggcag cgggtattgg aggagttaat ctattcgaac atatcgctgg tatgcgttat
     4381 acggatttcc cagcattcgt cctttcgttg attttttata cgatattagg acataacagt
     4441 cattcagccg atctagctag tatcaatgaa atgatccaaa acatgaaagc gagcttttgg
     4501 atctctcctt ggacattgat tccactagtt attttatttg ggggggcaat caaaaaaatt
     4561 ccggctatcc ctacattact agcgggatcg acagctgctc tcgttctttc ttttatccat
     4621 gagaaaagtt tgacggtcgg aaaagcagca gacatcttga tgaatggtta tgtcgcaaat
     4681 acacctgatc caaagctaga tgaactgctt tctcgaggcg ggatcatgag tatgttaggt
     4741 tctgctagtt tgattttact tgcattggca ttaggtgggt tactgatcaa atatctgatc
     4801 gttgatacca tcgttcaaga attaaaagaa aagatggacc gaccttctcg tttagtcggg
     4861 tttactgcct tgagctgtat cggaatcaat ctgatcgttg gagaacagta tctgtccatc
     4921 attcttccag gagaaacatt caagcgttct tttgatgacg ttggcttatc aaaaacctat
     4981 ttgacacgaa cactagcgga ttcaggtgcg accgtcaatt ctctcgtacc gtggggagtc
     5041 agcggcacat tcattatggg aacgatgaaa gttagcgcgt tgcattactt gccattcgcc
     5101 tttttctcga ttcttgcacc tgtctttaca atattaggcg gttttttatt gaatcgtcga
     5161 cgtgtaaaaa attgaaggaa ggaaaaaaga ggctgggaca gaagcgttta actccaagaa
     5221 ataagaagaa actcaagaaa attgcttttt aaatttttgt gaatttcagc ttatttccga
     5281 aggagttgct tctgcttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     5341 tacttttgtc ccacgctctt ttttacagat aaacaatgtg ttagaagtag tatcttcttt
     5401 tacataaaaa gctgaccata ggataaatgg tcagcgtatt tagtgagtca gctatttagt
     5461 atgggttttc taaaggaacg actgtctctc ttttgatcaa gcgatgaggg atgaccaaac
     5521 gtacgccact gctcaattct tcatggatca attcgcgcag cttttgcata gcgacacgtc
     5581 ctaattcgga gatatcgata tcgatacttg taaggtaagg gtgtgttaat gttgagaaga
     5641 tcgagttgtt gaagctaatg acggagagtg tctctggaat gacatagcca tacatttgcg
     5701 ccaactgcat cgtgcgaagg gcgaagatat cgtcaatgac caccaatgct gtggcttcgt
     5761 tttctttcaa cgttttctca aaggacagat aatcctccgg atgctctagt actacactgg
     5821 ataacgcagg aagaccagcc atcatcattg ctttttgata accgaaatat cgttcgaaat
     5881 acaaactttc atgagtcgtg tttgtcacaa acaggatatt cttatgtcca ttctggatca
     5941 aaaattctgt tgcttgttta cctagaagct ggttatcatt atctacataa acgatttcgt
     6001 tctcatgatg ataaggctgt ccgatcaaag tgaaagggat gtggttttgg tataaataat
     6061 caatgacagt atcttgacta tctgaataca ctagaataaa accatctact tgtttttgta
     6121 aatgcatacg ctggacgttc tctaacagca attcaaaatt tttagcagta gcgattgctg
     6181 tagtcatatc ataattccgc gcttcttcgt tgattgcttc catgatctca agataaaatg
     6241 ggttaccgat ccgctctttt gaatcaagag ggggtaagat tacgccgatg gcactggaaa
     6301 tgcgttttcc tagattgcgg gcagtcatgt taggaacata acctagttca tccataattt
     6361 ttcggacttt cttctttgtt gtctcggaaa tactaggatg atcgttgatg acacgagaga
     6421 cggtcgaggt ggcaacaccg gcttttttcg ccacatcttt tacggtaata gccatgataa
     6481 aaacaccttt ctcttgttat tgtgcagaga accgataaat cgtctgactg atccgttctt
     6541 gaccaggatg gaggttgatc gatccaaatt tggggaaatg aacgatatca gggatttctt
     6601 gcggttcgat tgctaatccg tagtttgaat gcatctgttt tccattgaca gagaaatcag
     6661 cttcgaatcc agtcgtagaa aataatacca tacttttatt agaagttgct atagtcattt
     6721 ggcgaccaga atgcttctcg aataattgta aggaagtttt ggaaagccta ggatgatgca
     6781 aaagaaatac atcatctagt ccatctggat aacatgcaag gatctcttgc agtgttgggg
     6841 attttctaaa gtcgattggc agtttttctt tttttgtcca atttccagta ggcagttttt
     6901 cttgatctaa ttcaagcaca ccatctaaaa agaccgttaa ctgatgttca tagatgtctc
     6961 ttgttccatt tcctgaaagg ttgaagtagg catggttggt cggattgcag atggtttcaa
     7021 caaggctact agcagaggtt gtcatctcta attcgttttt tgttaaacgg taagtgatcg
     7081 tagcagtgat tggtcctgga taggtagaaa attcatcttt taagtaaaag accacaccta
     7141 ttgatgatgg agttttgaaa acttctactt cccaaaattg gaaagaccag cctttcgtgc
     7201 caccgtggat atggtgtgat ccgcagtttt tctccaatct atgctcaccc caagaagcat
     7261 cccggatcct tccggctaca ggtccgacag ttgctccaaa aaagcttttg tctgttagaa
     7321 catcttcgaa acgatcgtaa gacaataaaa cattttctga ttttccttct ttgtctggtg
     7381 cgaagatttg gtgcaggcgg gcaccgtaat tgagaagaac gacttctaag tagtcgttct
     7441 tcaatgtgat tttctttatt tcagtctctg gaactggtac gacggagatc tctggattat
     7501 ccatggtcat gccacttttc tttcaagtaa tcgatcgtaa gaaaagtcgt atcagggaca
     7561 agcgggatct cctctccaag gtcttcagca cgtcccacgc caattggttg agcgccactt
     7621 gagaggatgg cttggatacc ggcttgcgcg tcttcgatcc cgatacattc ttctgctttt
     7681 agtccgacag cttttgctgc aaggaggaag atatctggcg caggttttcc gtttgttgct
     7741 tctgccgggt cggcaatagc atcaaagtaa ggtgtaagac ccattttttc aagtaggaat
     7801 ggcccgtttt tacttgcaga agcaagagca atcttgattt tttcttcttt taaagaatcc
     7861 agtaaaggca cgaccccggg gaagacatct tttggttcga tcgtctggat catctctaaa
     7921 taataatcgt tttttctttt cgctaattct gcaaattctt cctttgaaaa agagtgttct
     7981 tttccgccat aagctaaaag caaagagagt gagtcttctc ggctgacgcc ttttagctgt
     8041 tcgttgaatg tgtggtcgat cgtaatgccg atttcctgac caagctttcg ccaagcacga
     8101 taatggaatt cagcagtatc ggtaatgaca ccgtctaaat caaacaatac ccctttaaac
     8161 atttgcttcc tcctttctta gcgttatttc tagacgatcc gctaaagaat aagactgttc
     8221 atagacgtaa atttctagag gagaaccttc taataactcg aaagtaactt gttcatctgt
     8281 gactgagatg aacaataaac gtccgcgata attgatatgg aaagaataag ctttccaagt
     8341 ttttggcaga aatggtgcga atctcaactg atcatgggca gttttcattt gagcaaatcc
     8401 ttggacaatc gacaaccagc taccagtcat ggaagtgatg tgcaagccgt cttctgtgtc
     8461 gttattgtaa ttatccaaat ccaagcgagc tgttcgctgg tacatttcta cagctttgct
     8521 ttccattcct aattctgctg ctaaaatcga atggatactt ggcgaaaggg aagactcatg
     8581 gacagtcatt ggttcataga aatcaaagtt tttttgcttt tcttcttttg taaaagtatc
     8641 attgaagaaa taaatcccct gtagtacatc tgcctgcttg atgaagcaag atctaaggat
     8701 tttgtcccaa gaccaatgct gattcagagg aagatcgctt gtgggaatat cggatacagg
     8761 catgagatct ttatctaaga atgtgtcatg ctggacaaag attcctaatt cttcgtcaac
     8821 tgggaagtac atgttctctg cgatatcttt ccatttggct agttcttctt cgctgatagt
     8881 gatagcagct tctttttgat aagttaaata attttctcgc gtgtaacgca agacccaagc
     8941 tgcgatcgta tttgtatacc aattgttatt gatattgttc tcatattcgt ttgggccggt
     9001 cacaccgtgg atcatgtatt tatttgctcg ttttgaaaaa tggacacggt ctgcccagaa
     9061 gcgtgcgatt tctgctaaaa cttccagtcc ttcatttttc aaatattccg tatcgccggt
     9121 gtaattcgta taattgtaga ttgcataggc aatcgcgcca tttcggtgaa tctcctcgaa
     9181 tgtgatttcc cactcgttat ggcattcgac accagtgaag gtgaccatag gataaagcgc
     9241 gccttttaat ccttgttgct gagcgttgtg gattgcttgc ggcagttgat tatgccgata
     9301 tttcagcaga ttcttagtta cttcaggttt ggctaaagca agataaagag ggacagcata
     9361 cgcttctgta tcccagtaag tggctccgcc gtatttttct cctgtgaatc ctttggggcc
     9421 gatattcaat cgttcatctt cgccgtagta agtagagaat agttggaaca aattgaatcg
     9481 aataccttgt tgtgcttctt cgtctccttc gatgaccaca tcggcaagct tccatcgttc
     9541 tgcccaagcc gctgtttgct cttcttttgc ttggatataa ttgtttgtct ggttcgttaa
     9601 ttctgagaca gctttttctg cttgctgcgc ttctggaata tctctactag taagaacgat
     9661 aatttctttt tccactgacc atgtctcacc ttgagataat tctgcagaca agcaaccatg
     9721 taatgctaaa gtatcttcgt gatagtcagg gatgactgat tttccattaa cgaaatggcg
     9781 cattgctgca gtaacagtaa attgttcaat cccaaagttg tttggaatag ttttagtcgt
     9841 caaatagccg atttcttcta tagttccacg atcaatctca ttccaaaaat gttcatcata
     9901 gttgctgtct tcgttttgaa catttccatc caattttgat acgagtttga ctgttccgct
     9961 accttcaagc atttctatag ttaaatagat ataagctgat tcttttttga caatgctcag
    10021 aaaacgttcg aaggaaatgc gtactttatt agtagaagta gtgattgtga atgtacgaga
    10081 taatacacca tttttcatat ctaattccca gtaaaagtct tctggttcca aagaagccaa
    10141 gtcgatcagc tgatcgttga tcgaaatatc tatcgcgata aagttcaagg cgttgatcac
    10201 ttttccaaag tattctggat aaccattttt ccaccagccg acacgtgtct tatcgggata
    10261 ccagacaccc gccaaataag taccttgatg gtggtcccct gagtaagttt cttcaaagtt
    10321 cccgcgcatc cccatataac cgtttccaat acttgttaaa gattcttgca gtcgaagatt
    10381 ttttttatct aattgtgtcg tgctgatttt ccatggatca atagcaaata gtcgtttgat
    10441 ctgtttcatt tttctcctcc taatgagctg ttttatacgt tcataataaa cgcaaccgat
    10501 tgcacaagtc aatagacaaa agaaaatatt tcatagaaaa aagcttaaaa aaataaattt
    10561 ttaggagaaa attatagaag ggaacacacg tattataggt tttatagaat ggtatagaca
    10621 gaaaaataaa aaataaagca tttacaaaaa agaaaacgca cgctataata cgtttaacga
    10681 aaccgattgc gtaaaggagc taaacggaat gaaaaagata tttagttttg agttttggca
    10741 aaaatttgga aaggctttaa tggttgtggt agcagttatg ccggcagctg ggttgatgat
    10801 cagtataggg aaatcaattc ccttgatcaa tcctgattgg acgccgcttg tcacaacggg
    10861 cggagtaatt gaaaatattg gttgggcaat tattggtaat cttcatattt tatttgcttt
    10921 agcaatagga ggaagttggg caaaagaaag agcaggcgga gcatttgcag ctgggatctc
    10981 atttatttta atcaatcgga tcactggggc tatctttggt gtcacaagtg acatgcttgc
    11041 gaatgaagat gcatttaccc acactctgtt cggtacgaag atcatggtta aaggattttt
    11101 tacaagtgta ttggaagcac cagctttgaa tatgggagta ttcgtaggga tcatcgctgg
    11161 atttgtcggg gcaatggctt ataataagta ctataattat agaaaattgc cggatgcttt
    11221 gtcatttttc aatggaaagc gctttgttcc gtttgttgtt atcttatggt caacaatcgt
    11281 ggcattgatt cttgctgtgg tgtggccaaa cgtacaggct ggaatcaata attttggttt
    11341 atggattgca gaatcacaag aaagcgcacc gattctagca ccgtttttat ttggaacatt
    11401 agaacgttta ctattgccat ttggattgca tcatatgctg acgattccta tcaactatac
    11461 acagcttggc ggatcgtatg agattttatc aggcgcacag gcggggacac aagtatttgg
    11521 gcaagatccg ttatggttgg cttgggccac tgatttagtg aatcttaaag gtgcagggga
    11581 tatgtcgcaa tatgagtttg tgttgcataa ctggacgccg gctcgtttca aagtaggaca
    11641 aatgattggt tcttcaggta tcttgatggg aatggctttt gcgatgtatc gtaatgtcga
    11701 tcctgataag aaagcaagat ataaatcaat gtatttttca gcagcgttgg ctgtctttct
    11761 aacaggtgta acagaaccat tggagttcat gtttatgttt gctgctgtgc cattatatgt
    11821 catttattca gtgatccaag gggctgcatt tgcgatggca gatatcttgc cattgcgggt
    11881 ccattcattt gggaatatcg aattgctgac tcggacaccg ttagctatca aagcgggtct
    11941 aggtggagat cttatcaact tcgtgttgtg tgtgatcgct tttggtgttg ttacgtattt
    12001 cttagcaaat ttcttgatca aaaaattcaa ctttgctaca ccgggacgta acggaaatta
    12061 cgacaatgac agtgaagaaa ccgttgtatc taacagcgga acaggaacag cagatcagca
    12121 agtggtccag atcattcatt tgctgggagg aaaagaaaat atcaaagatg tcgatgcttg
    12181 tatgactcgg ttacgcgtaa gtgtaaatga tcgagaaaaa gtagggactg aagaagcctg
    12241 gaaacgagca ggggcgatgg gattgatcgt caaagacaat ggagtccaag cagtttatgg
    12301 accaaaagca gatgtcttga aatcagatat cgaagatctg ctgcaatcag gagcagagat
    12361 tccagagcca gaaatggttt ctacaggaaa tggacaagca ggtagtaagc aatacttagg
    12421 tatagaacaa gaactagttt ccgcagcttc tggagaagtg attcctttat ctgaagtaaa
    12481 agatccggta ttctcacaaa aaatgatggg tgacggcttt gctgttattc caacatcaag
    12541 agaagttgtt gcgccgatcg ctggaaaagt gacaagtatt ttccctagca aacatgcaat
    12601 tgggctggaa acgaaagatg gtatcgaagt tttgatccat atgggaatcg atacagtcca
    12661 aatgaatcag ccagcttttg agattgctgt caaagaaggc caagaagtag gaacaggaac
    12721 gaaattagct gagatggatc ttgatgtgat ccaaaacgaa gaaaaagatc caacgatcat
    12781 gatcgtattt acaaacgata aagtagaaga agtagtgatc aagcaattag gtacagtgac
    12841 agctggtaaa gtgatcggct ccattaaaat gtaaggagag gttgtgaaaa agctgtcctg
    12901 catcaagtca taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggttgggc
    12961 ttattaccga gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc
    13021 ctgcaccaag tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta
    13081 ctgaggagtt agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca
    13141 acccttttgt ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac
    13201 gcatagctgg cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct
    13261 gttagaagat tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga
    13321 agtagaacct gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa
    13381 agataatttt gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg
    13441 gagttgggaa atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac
    13501 gaaaaaaaca ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta
    13561 tcagacacgc aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc
    13621 gagctgccac ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa
    13681 cttggaaaac tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa
    13741 tccagtagac tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc
    13801 gtatgttgtc gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg
    13861 ggaacaaaat acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt
    13921 taaaaaatat caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg
    13981 ggtggaaatc gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg
    14041 attaaaataa aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt
    14101 tatccctttt gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa
    14161 ggtaaaccag ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata
    14221 atgaagcgtg agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt
    14281 tacttcataa tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat
    14341 aacagggata tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc
    14401 tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta
    14461 tttattgtct tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac
    14521 gaacaagctg gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc
    14581 gaatagataa gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta
    14641 taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa
    14701 ccacttttgt tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt
    14761 aaatggcacg aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc
    14821 gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta
    14881 caacacgatt ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc
    14941 tgataatatc aatataggaa aggccttagg gtaatggagc caataactgc catccagatc
    15001 tatcgccttt tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta
    15061 aacaatagct aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt
    15121 aatcagttga agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa
    15181 agggatatac atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc
    15241 cactgagaca gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg
    15301 gatcatacat aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca
    15361 gtattgaata gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg
    15421 aacattgaca tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa
    15481 atttgaaaga atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa
    15541 ataagcgaaa aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata
    15601 aagggaagga aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg
    15661 attgtaatgg aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat
    15721 gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg
    15781 aactcctttg caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa
    15841 attagaaaat tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta
    15901 aggagagagg atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct
    15961 taatactcga aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg
    16021 cttctcggta ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct
    16081 cttattactc aaagcataac gcagttttca caacctcttc cttaaacggt gttcaaaagc
    16141 ttgcatctgc ggtaataagc ccaacccaac aaaaaatatg agtagctatt tttttgggct
    16201 gtacttatta cttgatgcag gacagctttt tcacaacctc atcggctttc attttctatt
    16261 tttttcctga acccatataa taaaagaaag aacaaaaaaa cttgcaaggg aggacacatg
    16321 tgaagcagag taagcaaaaa cagtctcgtc gacttttgtt tcgtttgttt ttattaatat
    16381 gtatcgtcgt agggttactg atcgctttat atccatttta tgtggattct ttaaatagtt
    16441 ttattgatca aaaacgtatc gaacaagtac aaaaacggac cgcagctgaa attgaagtgc
    16501 agcgaaagaa aatggaggag aaaaaccaac gattaactga ccaaggattc aatcctggta
    16561 ctgatccttt caatgaaaag aacagaaatg aaagtacaac atccagccaa ttagaggaat
    16621 ggctgatcgg aagtatcaat atacctaaga tccaattaaa tatttcactt tatgatcggc
    16681 tcaatggtag gatccttgaa aatggagcag gcgtcttgca aggaacctct ttcccgcttg
    16741 gtggaaattc gacgcattca gtgatctctg ctcacagcgg tttacctaat cgacggttat
    16801 ttaccgaatt agatcgttta gaaaacggag acacgtttat tttgactgtt ttgggggaga
    16861 aattggctta tcaagttaaa aatatacaag tggtactgcc agatgacacg tctgttctaa
    16921 cgatttcaga aggagaagac ttagttacct tgcttacatg tacgccgtat atgatcaaca
    16981 cgcatcgttt gctagtgaca ggacatcgga ttccttataa cgaatcggta aaaaaagaag
    17041 aaggaaaagg aaatcaggaa agaaccatgc ggcaatggtt tattcttgca ggaacgataa
    17101 tagctgtagt catactgtta gtgtttatcg gacgactaat ctatcagtat agattaagta
    17161 aaaaagtgct tgatttttca tttattatta gtgattcgtc agggaaaccg gtaaatggta
    17221 gttcattcat tttgaaacat aagaaaaaaa cacttactag aaatggtgtg ccgttttcag
    17281 tacaaagcga ccatcatggg aaagtcaagc tggatcaatt gcctggtgga acttatcgaa
    17341 tcgtttcagc tgatgatcct aaagttgctg tttcttttgg tattagaaaa gtaaaacaag
    17401 agaagatata cttttttgag gggcgaaaac ttgtaaaaga gcttcaaaaa aatggttttt
    17461 attttaaaat caatgaataa gtaaggagag aaaaaatgat ccgcacaaca acaaaattgt
    17521 ctgccaaaga actggaatct gttctatcga tttggctgca tgctaacttg gattctcatg
    17581 aattcattcc atcaacttat tgggagaatc aagtagaatc tgttaggaaa gctttaccag
    17641 atgcagtgat ttacttagca gaaatagatc aagaaattat tggttttgct ggggtgattg
    17701 atcagtttat cgcaggaatc tttgtgaaaa gggaataccg gcaacaaaga atcggtacgc
    17761 aactaatgaa aaaaataaaa gaaaccaata aaacactgac tttgacagtc tatcaaaaaa
    17821 atcaatcagc tattcatttc tatctttcac aaggttttac aattcaaaca cagcaaaaag
    17881 atgaagcagt aaatgaggag gaacttgtga tgaaatggaa tcaaaacgct gatttttgat
    17941 gaacagaata acgaaataat gctcccagcc taattttttg ctgggagcat tatttcattt
    18001 tgaagtcact aatttaactg tgtgaatatg cagttgttac aatctttgat aaacggtgtt
    18061 caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc
    18121 atttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttga taaacggtgt
    18181 tcaaacggct gcttctcggt aataagccca ctagcctaga aaatatgaaa agctattttc
    18241 taactagcgt tcttattact caaagcataa cgctgatttc acaacctctt gataaacggt
    18301 gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaacatgag gaactatttt
    18361 tccgtttctt tctctaatac tcaggtcaga acgctgattt cacaacctct ctttcacaac
    18421 cttattttac tcgtttataa ttttcatcga agtattttcc gctacggata tcatctggca
    18481 ttccatcgta tggttcttcg ctaataacat cttcattgtc tatacctgca tcacccatat
    18541 aagtgatcgt agcaaattca ggaacaagta attttggata agtttcatat ttttcacgag
    18601 ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac agtgatttct ggatgttctt
    18661 caacccattt agggaaaaag atcattccgt gaagtttctt ttcatttggc ataaagtcca
    18721 atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt gaatacgccg tccgtcaatt
    18781 taacgatatc agcttcttgg tcttttaccc aacggccttt gaccattccg ccatggatac
    18841 ggtaatcaac agtatggtca tttttggcat accattcata ttcccaacca ttgtcatacg
    18901 tatagataaa gtgagttcca ataaaatctt ccaattcttt aaattctttc atgtttaagc
    18961 atctccttat aaattaaaac atgtaataaa ctacatgttt tcgattactc atttatgata
    19021 gaatggattt aaagatttgt caaggaggga aaactgatgg caagaaaaaa tacacttcga
    19081 tatattttgt tagggttatt aagtaagaag aagatgacag gatatgaatt gaatcaatcg
    19141 ttcaaaaatg aaataggaga attttggcaa gcaaaacata gtcagattta tcctgaactt
    19201 gcaaaaatgg aagagcaagg aatcattcag catcaagtag agatcacagg tgaaaaattg
    19261 gaaaagaaag tttatgaatt aacagaagaa gggaaggaac aattggcaga gtggattcat
    19321 acacccacca atgaactacc ggttaatcga gacgaatttg ttctgaaatt gtatttcgtc
    19381 aaagacgtga atgatccggc attagtagaa attattcagc agcagcgtga ccttcatgaa
    19441 gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct ttcctactga aaaagaaaaa
    19501 catgaaaatt acggacacta cctgatttta gcgcatgcga tcaatcggga aacagaatat
    19561 gcaaattggc taacagatgt attagaagaa agtaaccgaa aaaaataaaa aaatatccga
    19621 acagtgatgg agaattcctt tctatatgaa agaaacgcag atcaccgttc ggatattctt
    19681 atgttcaaac aggccttttt tcaagtattt atgtagcatc tacttattaa aaagcttggt
    19741 acttaggaga ttagtggcgt ttagagttct tttaacaact gttcgtagtc agctttttca
    19801 tcgacaggaa ggagcttgta agttaattcg tctgtctcgt tgtcatgaat gatgtacatc
    19861 ttagcattct cttctttgaa gttaccgatt tgtaaaatcg tatagcgttc gtcgatcaat
    19921 tcgatatctt tgattgcttt tgaaaaaact ttacggacat ggtgcatttt cttttggcgg
    19981 atttctgcct tttctgcagc ttttgacaaa gtaaatccgt catctaaata ttttttgatc
    20041 agttctactt ttatgactgt aggtaaacga tattttctag gacaattcgc atcagcttcg
    20101 atcgaacgaa tgaatccctt ttgttcccaa taccttaatt gacgtggaga cacacctacg
    20161 atatcactta attcactgat gccaacaacc aattgatcat tttccaacaa acgtttaagg
    20221 gaagccccaa ccatttttgt cactcctttt gtcctcttgc ttcccttaat tatatccgta
    20281 ttaacttatg tgtcaagtag gttgacaaat agcgaattta ggactaaagt attctagtag
    20341 tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac aaccaacccg tagatattta
    20401 cggaaaacca tataatcgga cacttttagt tgttgtctta ttaattggta cattttgtac
    20461 cgttttgaat cagaccttat tgacgacagc ttttcctaca ttgatgaaag ctttcgatat
    20521 ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg ctagtcaacg ggatcatgat
    20581 cccaataaca gcatggctga tcaataaatt cagttcaaga aatttatatc tttctgccat
    20641 gacgatcttt ttgatcggaa cgattacttg ctttacagca cctaatttta gtacattact
    20701 aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg atgccattat tgcaaaatat
    20761 catgctatct attttcccgc cagaaaaaag aggatcagca atggggatgt cagggatcgt
    20821 tattggactt gctcctgctt taggccctac attatctggt tatatcattg ataactacca
    20881 ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt cttgtcttgg ttttagcatt
    20941 cttcctaatg aaaagtgtta ttccattgtc aaatcctagc attgatattt tatccgctat
    21001 tttatcaacg attggattcg gtagtttact ttacggattt tctagtgtag gagatgacgg
    21061 ctggggaagc ttgaaagtaa ttggtttctt agttgttggt gtgattttca tcgccttgtt
    21121 ctcatggcgt caattacatt tggaacatcc attcttggaa cttcgtgtat tcaaatcatc
    21181 tacattcacg attgcagctg ttttagctgg tgtaacaaat atggcgatgg taggggcaga
    21241 aatggtcgta ccattgtata ttcaaaatat tcgtggagaa tcagcattcc attctggctt
    21301 gatgttgttg ccgggtgcat tgatcatggg ggctatgatg cctgtgacgg gtgcgatttt
    21361 cgataaatac ggcgcgaaac gtttagccat cacagggatg ttcattttga cagcggcaac
    21421 tgcaccattt gctttcttga caaaaacgac tccagtttta tatatcgtga tcctttatgc
    21481 gatccgtatg tttgggatct ccatggtcat gatgccggta acgacttctg gtatgaatgc
    21541 attgccaaat aacttgatca gtcatgggac agcggtaaat aatacattcc gtcaaatcgc
    21601 aagctcgatt ggtacagcta ttttgatcag tgtgttgacg aatgtgacaa aagcaaatat
    21661 cccaagcaaa tctgtattgg ataatacgcc attagcttat aaagatcagg caatcaatgc
    21721 cacactgtca ggttaccatg ccgcgtttat cgtagcgatc atctttggga ttcttggctt
    21781 tattattgcc tttttattga agaaaaaaga acctgttcta atgaaagcgg gggataatgc
    21841 atgattattg gagttttgat tgtcagcttg cttgcttttg ctttaacgaa cgtgttcgct
    21901 aaaaaaacat ggcaaacctt tttatctttg attttcggac taatcttcgt cgcttcacta
    21961 agtttgatcg tcgctaattt gtccaatcac tttggaatgg aaaaagtaac ggaaacaaaa
    22021 acagaaaaaa ttgtttccag tgctgattcc caaggagcag atatgctttt gtataaagct
    22081 ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa atgaaaaaca agaaaaacca
    22141 aaagcaactg gtacggataa cgaaacaaat aaagtagaaa agacagacgg cgatgctgaa
    22201 aaagtgacga aaactactta ttgggaatac aaaaacgaca tgtataaatt ctggttcaat
    22261 attgctgata acaatcacga atatgacagc cgagtgaata cattcaaaat cccagagaca
    22321 tgggtagaat taagtacgga ccaagcagcc aagttagctg aattagtgaa gaaacagcaa
    22381 agtacgatgg aaagtgaagc aaaagcttat gttcaagacg gtatggtaaa agcaatgact
    22441 gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta cgaaagcctt agctgctgaa
    22501 ttccaacaac aagcattcgc aaaattagtg aaagaagcaa aaggcgaata atattttgaa
    22561 caccatgtag gaggttgtga aaaagctgct ctgcatcaag cagtaagaac aatcagataa
    22621 aaatagcact ccatattttt catctgattg ggcttagtgc cgtagatgca agcttttgaa
    22681 caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggagcaattt
    22741 caaaaaacag cttctcatgt ttgttgacta ttgtgacttg tgaccgagga gttggcagga
    22801 gaacaccgtt tattcgaaaa aaggaactgc gacaagactt ttgtcacagt tccttctttt
    22861 ttttgctatg ctatactaga tgtaaaaaat aggagaaata aaatgaggga aatttattct
    22921 tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg gggcatatat tatttttccg
    22981 tatgatataa gaaaagaatt cggtgctgga agagttaaag tccaagcaac ttttgacaat
    23041 gtgccgtatg aaggcagtat cgtgaatatg ggcgtgaaaa atccagatgg ttcagtctgc
    23101 tacatccttg gactacgaaa agatattcgt aaaaacatcg gaaaagaaat cggtgatatt
    23161 gtagcagtta cagtgaagca aaaataaaag aggttgtgac aaaagacgtg tcacaacctc
    23221 ttgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat gcaaaaatgt
    23281 ggaaacccat gtttacaaaa tttttcttac tactcgaaac gaagcggctt tatcacaacc
    23341 tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta gctcaaaaac ggcaaaatat
    23401 gagaagctat ttttccattt ttttctctca tactcaggtc ataacgctga tttcacaacc
    23461 tcttctttca taatggtttt tcaccatctt tataataaac tggttatttg atatggaaat
    23521 ctattttttc caaccaatct aaaatcttgt aacctaactc ttctgacatc ggaaaagagg
    23581 cgttgatttg tgcattggag atatggtcaa catggatacc ggaagtgatc gtacaacttc
    23641 ccggaacggc agttttgatg atcacagcta ttttttcggc taagacatcg tctttatgga
    23701 accgaccatc gtggctaggg aaacgtacag tttgcatctc tgtatcacga gaaaatgtag
    23761 tgactgttcc gatatgtggt gtatctccgc ctgttaaagt gatcaataag tctttaccga
    23821 ttactttaac agcagcttgg atcgtgaaac ctagtttggt tacttcaact atcgtttctt
    23881 tttccatatt aatcaccctg ccaacgtttt gaatgctcat ttacaatttc aaattgatcc
    23941 aaaagtttca ttgtatggtg attaacaaga tcttggatcg tttgaggctg attataaaaa
    24001 gcaggaattg gtggtatgat ttgcacacct agtcgtgcta atttagtcaa gttttccaag
    24061 tgaatggcat ttaatggcgt ttcacgagga acaatgacta actttcgttg ctccttcatt
    24121 gttacatcag cagcacgtgc aatcaggttg tcgcctaaac caacagagat acctgcgact
    24181 gttttcatac ttgccggtac gatgatcatt ccatcggtca aaaatgatcc gctggcgaca
    24241 gcagcaccta aatctttgtt tgaataatgg taatcaaaca ggctttcgaa ttcagttagt
    24301 gataagtcgg attccagctt taagttctgt tttgcccatg gactgatgat tccgtgtgtt
    24361 tctatcatcg gatattcttt tagtttcttc actaggttga tggcataaat ggtgcctgaa
    24421 gcgccactta caccaatcac gatttttttc ttccgttgtg ccaattgttc cattttccgc
    24481 actccttttt atgatgtcaa aacagttgca gatcattcat tatttcaaat atttttccca
    24541 atcttctact tcttggaatt ttgcacgtac aaaatgttct ttcatatcga aaggaactgt
    24601 accgtcaaag atagtttttg cacttatccc acggaaacga atagagccag gattatattc
    24661 tggtaattct gatgggtcca atggatggtt cctcattcct gggatcacga tgatatcttt
    24721 atctccttgg aaacgagtgt tgaaagtcca aactacatcg ttcatgtcga aaatatcaac
    24781 atcttcatcg actaaaatga ctgtcttcaa ttctttgaag gctgataaag caagaatagc
    24841 tgcttgacgc tgaatacctt catccgcttc gttttcttta cggatttgca tgatggtcat
    24901 caatttacca ccacctgcag gaggattgta gacatttact acttttcctg gaatagcttt
    24961 atcgaccaag gcaagaatac ttgcttctgt aggaataccg gccatagata cgtgttcttc
    25021 actgggacca atcgttgttt gcatgattgg attgtctttt cggtgagtaa ctgctttgac
    25081 tttcactact tgtacagctg gattagcatc cccagtatag ccaggaaatt ctggcattgc
    25141 ttttcctgag ttcgtgttga tatcttcttg cattgtttca tttggcatga tttctgcttc
    25201 aatgatgaat tcagcacgag caatacccac ttcatcgact gctataccat caacgatttg
    25261 aataggttcg ttacgcaaag ctcctgcaca ccatagttca ttgtagccaa gtggagtggt
    25321 aggtggttcg aaagtcgtgc caatggcaat tgctggatct aaaccaatat tgatagtgat
    25381 aggcattggt ttattcaagg cttcgaattg tttttggaag tgaccaatgt ggcgtccgcc
    25441 aggcatgatg tacataccga tcgtatcttt atcttctaga accatacgat ggatcgttac
    25501 atcgctcatt gttttttctg gatctgatcc taagactaca cccattgtga tgtatggacc
    25561 agcatcttca ggagtatttg taggtgcagg caaaatgttg cgaatatcga agtctgcatc
    25621 ggtagctaga tgaacgactt cttgtgctgg agcgtctgct ttgtctactt tcacaggttt
    25681 gattggattt tctacagcgt ctttcaagaa acgaccaagt gttcgataat catggtgcaa
    25741 aatttcacct acacgtttac gactagccat tgttccgatg ttcacacgtg tgccggggaa
    25801 acctttgata ttgttgaaag tcatagctgg tccttcttga gtcggacgtt ttactgtacc
    25861 gccagcaccg atataacggt aaacaccaga taattctgct tcaggatcga tttcaatatc
    25921 tgtttcgtga tattgacctg gaagtgtttt tagttcttcg atgacttttc ttaaatcata
    25981 cggttgatct gtcatgagag attcctcctt agttgtcttt acctatctat agtaaaaggt
    26041 gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt ctacaatgga atggatgcgt
    26101 ttttttgata atagaataaa ctatgatata cttatcacta aaagaaagcg catacttagt
    26161 gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc ctaaagcagt aaaagtggaa
    26221 aatatagcga atattttgaa agttacattt gaaaacggag aagtaaaata cgtgaaatct
    26281 cattggacag aagagataac cgatgccttg caatttggaa aaaaaggaag aggaaaaaga
    26341 aaaaatcttc tcgcactttc tcgtaatatg tggattggga ctgaagtaac gattgaagca
    26401 gatggaacag tattcataaa tggaaaagac aggtatacgc cagaagagtt gtggtacaaa
    26461 ggaaagaaaa gtataccaga attataaaaa ataattaaaa aaaacagcgc gaaacaagga
    26521 acatattact tcctcgtttc ttgctgtttt ctactatttc agcgctctta tgactcaaat
    26581 ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg atagcggcgt cttttttcgc
    26641 tgccattcgt gcaggaagat gaccgccaag catcgtcaat atagtagaga cgatcactag
    26701 aataatcgca tggacaggat ttagttgagc aacatttttt agatccgtca tgttgtataa
    26761 gatgctattg atagggaacg tagctagcca tgcaataaac acacctaata tcccagaaga
    26821 aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg atgtcttttt tacgtgcacc
    26881 taaagctttt agtacaccga tttcttttgt ccgttcaatc acagatgtat aagtaatgat
    26941 actgatcatg atcatacttg ttactaaaga gatccctgca aaagcaataa ggacataagt
    27001 gatagcatcc atcaatccgc cagtcagttc agtcatcgtt ccagctaaat cagtataaat
    27061 gattttatct tcatctgatt tgcctttatt gtaatcatct agataatcca agatcttttc
    27121 tttgtcttca aaattgttcg gataaatcat gatacttgaa ggcaagctgt ctccacctaa
    27181 ataactaagc aatgtttgtt tggcattttc gtcaactttt tcagtagtta agacatttac
    27241 accactgtct ctttgagctt tgacgatttc tgattctttg ttttcgttga cgatttgagt
    27301 cgttaattga tcgctataag cgattcctgg agataagaga ttcattgtag aagaagattt
    27361 cacgcgtagg atccccgaga tcttcaattc atcagaagca ttctgataca tcgcatcata
    27421 atctgtatta gggataaaat tcccagttgg aagttttgta tagtaagtat tgttgtttac
    27481 taatttgaat gttgtaccaa cgatatcatc aaaatcaagt ttttcgtctt ctttcacatc
    27541 aaatccaaga ttcttcaatg cgttgatatt agtattatta ttgccgtcta cgatcagcac
    27601 gacatctgtt gctgaggctg gataacttcc ggcaagtaaa gaataattgt cttttaaaaa
    27661 gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac actcccacac ctgtcattgc
    27721 tgacatcgta ctagataaag aaagagattc cgtatcagga ttttgattcg agaagctgac
    27781 aggctgtact ttgccattta catcacgtaa aagattgatt cctgtagtac gggtaaatcc
    27841 gatattatta cttaagttag gatcgatgtc tgttacgtaa tcgacgtatg tttgatcaat
    27901 cttattcgtg tgctgtgctt tatcttcttc gctgactttt gcagtaatcg ttttgctgtc
    27961 tggataatcc gctttgcttg cacccaatcc ggcatcgtca cgagtctggt tagtcgttac
    28021 cttagaaata gtaatcggaa atttcgccat cgtttcagct tgcgtgttat cgatttgttt
    28081 ttggaatcca gaagaaaggg acagaacgat cgcgattccg atgatcccaa tactggaagc
    28141 aaaggaggtc aagaaagtac gtcctttttt tgttcggata ttgttgaagg aaagctttaa
    28201 cgctgtccaa aaactcatct ttgttctgcg taagttaaat tgatcatctt ttggtcgttc
    28261 gatgtgagga tgggaatctg ttaagatctt accatcagaa aattcaatga ttcgatctgc
    28321 gtattgatgg gccagttcag gattatgtgt gaccatgatc actaattttt ctttcgataa
    28381 ctcttggatc aattccatga tctggatact tgtttctgta tctaacgctc cggttggttc
    28441 atcgcacaaa aggatatctg ggtcattggc tagtgctcga gcgatggcca ctcgctgcat
    28501 ttgtccacca gataactgat ttggcttttt atgcatatga tttgtcaagc cgactcggtg
    28561 caatgcatct tctgcccgtt ttcgtttttc gtctttagaa acaccactta atgtcatccc
    28621 aagttcaacg ttttcaataa tgcctagatg accaatcaag ttgtaacttt gaaaaatgaa
    28681 accgatagag ttgtttcggt aggcatccca gtcactgtcc ttaaagtctt tagtagactt
    28741 tccgttgatt accatatcac cggaatcata attatctagt ccaccaatca cattcagcat
    28801 agtcgtttta ccagaaccac taggacctaa aatcgctacg aattcttttt gtctgaaagc
    28861 gacagacaca ccatccaatg ctttggtggt ggtttcgccg actttataat atttttttat
    28921 atcttttaat tgaagcataa tcacccttct cccgtattga aatattcggt atactaataa
    28981 gtatatcaga acaatgatcg tagtatacga aataaatgtt aactgaagat gaactacaag
    29041 aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa tgaatcgtat tttagtagta
    29101 gaagatgatc cgcaattaca gttgctttac cgttctgtgt tggaacgagc gggcttttca
    29161 gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac tagaagcgac acgtatagaa
    29221 ttaatcatta cagatatcat gatgccgaaa atggatggct atgacttgct agactcatta
    29281 cgtagcacca gatttgacac acctgttcta atgattacgg caaaggccga ttttgaggat
    29341 aagaaacgcg ggttcaaatt aggagcagat gattatatga caaaacccat cgatgtcaat
    29401 gagatgatcc tacgtgtgga agcattgctt cgacgttcaa agatcaatta cagcagtagc
    29461 ttgataatcg gacaaacaaa attagagcta gatgcttata cagtgttaca agatcagcat
    29521 tcgttgattc tgcctcaaaa agaatttctg cttctgtata aattactgtc ttatcccaac
    29581 aagatcttca ctcgccaaca gttgatggat gatatttggg gattagatac gaatacagaa
    29641 gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc gttttgaaaa gacggaagat
    29701 tttcaaatca tcacagtacg gggactaggc tataaggcgg ttatttaaag atgaaaaaaa
    29761 agctattttc acaattatgg atctattttg ctagtattgt cttcatttcg atcttagtga
    29821 cactgctgtg ttttctcggt tttattttct tattgtctcg acattctatc tcgcctgctg
    29881 aatcggctgg aaaaccaacg ttatacccgt tattcgtctt tgcggggttc agtatggtcg
    29941 tgggtacagg aatatcgatt tttgttgggc ggcgtatcct gcatccaatc agtgcgctag
    30001 ggacaaatat gagtctggta gcaacgggag atttttcgat tcgaatggat gaacagcaga
    30061 aagtagcaga agtacagcaa ctgtataaag atttcaacgt tatggtccaa gaactcaata
    30121 gtatcgagac actgaggaat gactttgtgt ccagtgtatc ccatgaattc aaaacaccac
    30181 tagcaacaat ccaaggatat gttcaattgt tacaagcccc aaatctctct gacgaagaac
    30241 gccaaatatt tttatatcgg attatcgaaa gtatcacgca gctttcacaa ttaacagaga
    30301 acatcttaaa attgaacaaa ctggaaaacc agcgtatcca actggaaaag aaagagtatc
    30361 gattagatga acaaatcaga gaagtaatcg ttttttttca gcctaaatgg gagaaagaac
    30421 agctagaatt ggatatcgaa cttgctgcag taaattacac aggcaatgaa gagtttttgt
    30481 accaagtgtg gctgaatatc atggataacg ccataaagta caatcaaatc aatggacaga
    30541 ttcatatcaa actatttgag acagccacag agattgtact ggaagtgacc gattcaggtg
    30601 taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa attttatcaa ggagatacaa
    30661 gtcgtcaaat ttctgggaat gggctaggac tttcactggt taaaaaaatt ctagaactgc
    30721 atgatggacg tattgattat agcagtatcg aaggagtagg aacgacagca atgatacggt
    30781 taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt ctattgaagg tttttagggg
    30841 tttttagtcg tttttttgtg attagggaaa tgttcttacg gttcttttgg cataaaaatc
    30901 gctgctttct tattgaacta cgagaaatgt tagaggtgtc attgaaacaa ggacatgatg
    30961 caagaaagag agcgaaagtc atgcgtcgct ctcttttttt gggtggatag atggacagaa
    31021 aatgtagggc ttagttttct tcttgattta aatactccca aaatgtatct gaagccaaag
    31081 aatgctcacc gactttacga gtaaatgcaa gataactctc tgttgttgga gaaataggga
    31141 gaatcacagt attttctttt aatagattct tgactgtttt ttccataaca atagcaatcc
    31201 ccatgtcatt tgcaatcata ttgatgatca agtcgattct tgctccttta taagagattt
    31261 ttggttcaaa tccagcttcg cgacacagat ctagtacagg ttgatacaag ttcgtagtgg
    31321 ggccaaggag caaaaagttc tcctttttca gttcgcttag atccaatttc tttttactag
    31381 ctaaaggatg atttttaggt agaactgcta caaaatcgtc tgtttcagta ggcaaccact
    31441 caatcgaact tggtggggcc tggaaacttc tagcaaagtg gatcgtgtca ctatgttcat
    31501 tggcgtcatt gtaagttaat tcgacacttt ccacttcttt caactggact gtaaattcag
    31561 gatggcgttt caaaaactcg gtgattttgg aaaaaccttt ataattgatc attgtcggta
    31621 tcgttaagat atttaatgaa agattgttgt ctttcgtata ggtttcaagc acttcttgca
    31681 tagcatggta ttgttctacg acatttttta catatgggag caataagctt ccagcttcgg
    31741 tcaattcgat ttttctatgg gcgcgttcga ataatttgac gtctaattct gtttctaacg
    31801 aaagaatctg tttagaaata tttccttggg tggtgaattg tcgatcagca gttttggtga
    31861 aattcattgt ttcggctaaa tcaagaaaaa cttcgaattt atggatatcc atcatattcc
    31921 tcctacttat tccaataaag aatactttaa cacaaaaaat gaattggtgt aatcttctct
    31981 gtctgttaca atctttcttg cagcgaaagc aataattaca gaaatttttc tgattttttt
    32041 cgcttcgtag aaaaagagag gagttttttg atgccaactg gagttttgat caatgtaggt
    32101 tccgttttgt ttggcggact aattggcgga ttagtaggaa ataaattaag tgaacatttt
    32161 aaagcacagc tgacgatggt ttttggtgtg tgttcgatgg ggatgggaat ctattccatt
    32221 gcgccgatga aaaatatgcc ggctgttatt tttgctttag tgataggaac agcgattggt
    32281 ttgattgttc atttaggtaa tgggataaat aagggggctg cgctgatgca agtacctatt
    32341 tctaaaatct tccctagtga aaagcttggt atgacccacg atgaatttat cagcacatta
    32401 gtgacggtca ttgttctatt ttgtgcaagt ggtacgggga tctatggaag tttagattct
    32461 gggatgacag gagacagtac gattttgatt tctaaatctg tccttgattt tttcacagca
    32521 gctatttttg cgtgtaattt agggtatgtc gtttctgtga tagcaattcc ccaattcatt
    32581 attttttata ttttgttctt attggcaaaa tttatttatc cattgactac accagacatg
    32641 atcttagatt ttaaagcttg cggcggattc ttgatggtcg ctaccggttt tagaatgatc
    32701 aacgtgaaaa tgttcccagt agctgacatg atacctgcaa tgatcgttat catgccactg
    32761 agctggatgt ggacaaattg gattatgcca ctgttataat aaaaatcaaa gagaagccat
    32821 tttttgctga ctaaaaagga tcaaatcttt tagtcagttt ttttgtattt gacaaactga
    32881 ttttgaaact atattatatg ttgtataata tagattgaaa taaaatatta cacttgagaa
    32941 tagaaggtga cgtaatgcac atccagattc caactgttct tcttgatggt actgtgctag
    33001 cagcattaag caaggaagat atgtatgggt atgccttaac aaaaatcgta caagaaagtc
    33061 tctctgtcag tgaatctaca ctatatccgg ttttgagaag attgaaaaaa gaaggatatc
    33121 ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg atattactcg ttgacaccaa
    33181 tgggaagtcg tcatttagaa gaaattattg atgaatggat tgtttttcga gattctttag
    33241 accggttatt ggaggagagc acaaatgaat gaatatttga atgaagttgt ggatcatctt
    33301 caagaaattc ctgaagacga tcgttttgat ttgatccagt attatgagga atatttttta
    33361 gattctggaa aaacattaga tcagatcatt gatgaatatg gtacaccaaa gcagtttgcc
    33421 ttaaaattaa aaatcagtta cttttcagaa gaagaacaag cagaaaacga gttagtgaaa
    33481 aatccaaaaa gacaaattcg cctgatttgg ttaatcatta tcggtttatt tgcttctcct
    33541 gtcctcattc cactagcact ggggtttata ttggttattg ggggactatt ggttgcttta
    33601 ctagcgctca tttttagtat ctatgtttta tgtatcagta tcttaggtgt tggcctattc
    33661 atgatcatct caggaatcag tgtgtttgga caatcgattc tgagtggatt attctttatc
    33721 ggtgtcggtc tgttcgccac aggaagcgca gtatttattt cgccactgtt gctgaaagga
    33781 acaaaagggc ttttttatgt catgatgaac tttgttaaat ggatgggacg acgttttatt
    33841 acaaaaagag atctgcattc agcgggcgtt tgaaggagga cacataaatt gagattgaaa
    33901 cattttttga ttaccggtat tggtctgatg cttactggtg gcctcctagc aggtgcaact
    33961 tatacattag gcgctcaaaa gagtctgact tgggataatg gtcctcggtt gatcgatatg
    34021 gttactgaga caaaaaaaat caaggatgca aacaaaatcg tcatccatgc agaaaatcaa
    34081 acagtttcgg tcatacgcgg attggatttt gagataaaaa cgacttatga taagacagaa
    34141 aagccagaaa taaaggagaa aaatggcgtg ttgactgtga atgcagtcaa aaaatcaaca
    34201 ggtgcaatca ttagtttaga agacacaagt gcacaaatca ctattgttgt ccctgcagat
    34261 catgaattat ctgaattgac aatcgaaggg aataatagtg caattgatat caacgaagta
    34321 tcaatcaaaa aagtgaatac taagacacat gatggctggg tatcgttcaa tgaatttaca
    34381 ggaggaacaa taactgcgaa agatgaatta ggctcacttc gattagatga ggccaatgtg
    34441 gataacttga tcgtcacagg tgaaaacagc aacttccatc tagaagacag tttgatcagc
    34501 aaagaaggaa aagtcatcct aaacggtggc aatttatctt tgcatgagat ggaaaacagt
    34561 ggctatacta tccaaacgtc tggtgaatct tatattgaaa aagactacca gccaataaca
    34621 aatactttgt tagaacaagg acagaaaaag ctaaagatca ctgctaaaga cacatcgatc
    34681 aatattacga cggatcaaga ggacaatgaa acagattgat cctgtactca actggatatt
    34741 ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt tttcatcgct taacgcacat
    34801 aactatagta aaataagaaa gaaaatatct aaaaagaaaa cagggtgaaa gagtatatga
    34861 aggtagaaga aggtcaaaga gaaaagttaa aaacagaaat ggaaagatta catacttata
    34921 tcactcaact aagccaaacc ttctatgatc cagacaaaga aaaagtgatg gttaattacc
    34981 cgaacaattc agaaggtcgc caattagagc aagtgtacca tgaagttttc aaacatttat
    35041 tgacggtgaa gaaagagctg gattattatt ctttaccgat tatcgataca ggaatattga
    35101 agtatgatgg gaagaaggaa cgatttatct ttaaatctgt tcgggaagat ttgcctcttt
    35161 cagcagggat ggatctagag gtcttggtag aagactattt tacagaagaa aagcactggg
    35221 ttcgaacaaa attggattat ttaccgcagg cagccggagg gacacaggca agcggttggt
    35281 acatcactga agacaaagag ttagaactag aaggtgtaat ggcgcgtatt cgaaaaaaaa
    35341 attaaaaaag taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag
    35401 agaaaaaaga gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc
    35461 tttagttgga ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt
    35521 tttaagtatt tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag
    35581 tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa
    35641 tggctttttc cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa
    35701 tttcaatagt tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc
    35761 agtagtagtc tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca
    35821 agattttgta gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta
    35881 tgatacattc aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga
    35941 gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg
    36001 cttgaaccat ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc
    36061 aaaagccatt ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat
    36121 ggccttagag caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca
    36181 attgatcaat aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa
    36241 tacaggcgac atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt
    36301 aggacggatt attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga
    36361 aagaagaatg atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat
    36421 aaagtaagag agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg
    36481 ttgtggtact tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact
    36541 atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa
    36601 cagtcaacta tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg
    36661 acttcaaggg acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga
    36721 acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag
    36781 cactggaaaa actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa
    36841 tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag
    36901 cttcgatgta gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc
    36961 gacacttttg aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa
    37021 tcaaaaagat gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat
    37081 tggctttatt ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc
    37141 tgttcctctt tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat
    37201 tgcagaacgt ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg
    37261 acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg
    37321 ggatgagcct acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga
    37381 cgaattgaat acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc
    37441 aagttattgc caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg
    37501 tggagatcaa agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga
    37561 acagtagggg gagattgact atgctttgga aattatcact tactgggatc aagagccgtt
    37621 taagagacta cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt
    37681 ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag
    37741 ttatcatttt ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat
    37801 atgcaaattc ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg
    37861 gagcaaaagg cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg
    37921 cagctacttt agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat
    37981 tagtggatca gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt
    38041 tgatcactgt tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt
    38101 cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca
    38161 agcagcataa attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg
    38221 gttactatat gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag
    38281 ttacgatcgt attaggaact tatttcattt tccattctgt gatcattttc tttttatcat
    38341 tactaaagaa atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt
    38401 taaactttcg tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct
    38461 tggcactggg cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag
    38521 aaaacaattc tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa
    38581 tcaaagaact ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga
    38641 tttactataa tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg
    38701 gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg
    38761 tacaagagga acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag
    38821 tcagtcaatc agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg
    38881 taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga
    38941 acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt
    39001 ataatggtct attctctggt tttgaattta tgggattctt cctaggttta gctttcttga
    39061 ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc
    39121 gttatgaaat gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta
    39181 gagaagtagg cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg
    39241 gattacaaat gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta
    39301 cgatattctt cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa
    39361 tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag
    39421 caaaaatagc tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    39481 gaacaccgtt tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca
    39541 cggtctttta ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga
    39601 aaacggtttc catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca
    39661 tggaaatcaa tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa
    39721 atgggacatc tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa
    39781 tcgataatga aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa
    39841 aagaccctta tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat
    39901 tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac
    39961 atggtggtgc acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa
    40021 acgaagcatc ggtcattttc tatcagacta gcccagatat ggaaaacgga tttcctggaa
    40081 ctttagatgt agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc
    40141 aagggatcag tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa
    40201 ccggtgatcc agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg
    40261 ctacgctcaa taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat
    40321 ttgacttcca acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa
    40381 tgatagtggg cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg
    40441 ctgcggaatt gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag
    40501 gaatcgtgat ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat
    40561 tagtaaatca tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg
    40621 aaagttttgg agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca
    40681 aaataaatat aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt
    40741 tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc taaccttaac
    40801 aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt
    40861 cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg
    40921 ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc
    40981 catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca
    41041 gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc
    41101 gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt
    41161 gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca
    41221 gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat
    41281 ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg
    41341 aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg
    41401 cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat
    41461 gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac
    41521 tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat
    41581 gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca
    41641 aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca
    41701 tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt
    41761 ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat
    41821 tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa
    41881 gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca
    41941 agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat
    42001 acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata
    42061 ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa
    42121 ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg
    42181 tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt
    42241 gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt
    42301 gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct
    42361 ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa
    42421 tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta
    42481 atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata
    42541 tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc
    42601 aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca
    42661 gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg
    42721 tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt
    42781 gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca
    42841 gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata
    42901 aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt
    42961 ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa
    43021 taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat
    43081 tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt
    43141 aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag
    43201 aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat
    43261 aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac
    43321 taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg
    43381 tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac
    43441 cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt
    43501 aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg
    43561 aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa
    43621 aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga
    43681 tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc caaccaccag
    43741 cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta
    43801 aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga
    43861 taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga
    43921 ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg
    43981 tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg
    44041 aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc
    44101 cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga
    44161 cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa
    44221 tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac agcggaatga
    44281 tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt
    44341 tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat
    44401 ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct
    44461 gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg
    44521 cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa
    44581 tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc
    44641 actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca
    44701 gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa
    44761 caataatttg attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc
    44821 aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag
    44881 accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc
    44941 cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt
    45001 gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga
    45061 ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac
    45121 agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag
    45181 tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac
    45241 tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc
    45301 tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa
    45361 actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat
    45421 aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt
    45481 caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa
    45541 gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag
    45601 gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc
    45661 tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc
    45721 agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc
    45781 cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt
    45841 ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt
    45901 gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt
    45961 gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa
    46021 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
    46081 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
    46141 ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag
    46201 attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
    46261 tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat
    46321 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
    46381 tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
    46441 tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
    46501 ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag
    46561 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
    46621 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
    46681 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
    46741 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
    46801 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
    46861 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
    46921 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
    46981 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
    47041 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
    47101 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
    47161 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
    47221 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
    47281 aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat
    47341 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
    47401 gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca
    47461 tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag
    47521 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
    47581 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
    47641 ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt
    47701 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
    47761 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
    47821 tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa
    47881 ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat
    47941 ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga
    48001 gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg
    48061 attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg
    48121 gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat
    48181 aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc
    48241 gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg
    48301 cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg
    48361 tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat
    48421 cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa
    48481 gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca
    48541 cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta
    48601 aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat
    48661 tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga
    48721 cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa
    48781 actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct
    48841 gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag
    48901 atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac
    48961 gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact
    49021 gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa
    49081 gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat
    49141 accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg
    49201 aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca
    49261 gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc
    49321 aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat
    49381 cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca
    49441 cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat
    49501 gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga
    49561 acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa
    49621 ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa
    49681 aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt
    49741 tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt
    49801 ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa
    49861 aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa
    49921 aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta
    49981 ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg
    50041 agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt
    50101 gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca
    50161 ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg
    50221 ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag
    50281 tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc
    50341 gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac
    50401 tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat
    50461 atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa
    50521 ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct
    50581 gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa
    50641 acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat
    50701 atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa
    50761 aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa
    50821 tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa
    50881 atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa
    50941 caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc
    51001 gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc
    51061 gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg
    51121 atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt
    51181 gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt
    51241 ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg
    51301 gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg
    51361 aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa
    51421 acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc
    51481 tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat
    51541 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
    51601 agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
    51661 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
    51721 aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata
    51781 taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
    51841 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
    51901 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
    51961 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
    52021 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
    52081 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
    52141 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
    52201 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
    52261 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
    52321 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
    52381 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa
    52441 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
    52501 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
    52561 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
    52621 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
    52681 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
    52741 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
    52801 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
    52861 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
    52921 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
    52981 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
    53041 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
    53101 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
    53161 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    53221 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    53281 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc
    53341 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    53401 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    53461 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    53521 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    53581 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    53641 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    53701 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    53761 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    53821 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    53881 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    53941 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    54001 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    54061 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa
    54121 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    54181 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    54241 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa
    54301 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    54361 tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa
    54421 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    54481 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    54541 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    54601 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    54661 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    54721 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    54781 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    54841 aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg
    54901 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    54961 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa
    55021 agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt
    55081 tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga
    55141 ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt
    55201 tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga
    55261 aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga
    55321 aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca
    55381 tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat
    55441 tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa
    55501 acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt
    55561 tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc
    55621 gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca
    55681 ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat
    55741 tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg
    55801 attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct
    55861 cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa
    55921 aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct
    55981 ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga
    56041 tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga
    56101 atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat
    56161 ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga
    56221 aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa
    56281 ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc
    56341 cgaacatttt catttatatg ca
//
LOCUS       DAYYHB010000008        56243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000008 DAYYHB010000000
VERSION     DAYYHB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.008"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..1574)
                     /locus_tag="ACHA6B_000434"
     CDS             complement(6..1574)
                     /locus_tag="ACHA6B_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8948038.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1578..1988)
                     /locus_tag="ACHA6B_000435"
     CDS             complement(1578..1988)
                     /locus_tag="ACHA6B_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8948039.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1985..2908)
                     /locus_tag="ACHA6B_000436"
     CDS             complement(1985..2908)
                     /locus_tag="ACHA6B_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8948040.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2905..3783)
                     /locus_tag="ACHA6B_000437"
     CDS             complement(2905..3783)
                     /locus_tag="ACHA6B_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8948041.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3770..5107)
                     /locus_tag="ACHA6B_000438"
     CDS             complement(3770..5107)
                     /locus_tag="ACHA6B_000438"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8948042.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5110..6213)
                     /locus_tag="ACHA6B_000439"
     CDS             complement(5110..6213)
                     /locus_tag="ACHA6B_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8948043.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6287..7795)
                     /locus_tag="ACHA6B_000440"
     CDS             complement(6287..7795)
                     /locus_tag="ACHA6B_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8948044.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7761..8453)
                     /locus_tag="ACHA6B_000441"
     CDS             complement(7761..8453)
                     /locus_tag="ACHA6B_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8948045.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8475..9449)
                     /locus_tag="ACHA6B_000442"
     CDS             complement(8475..9449)
                     /locus_tag="ACHA6B_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948046.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9749..10621
                     /locus_tag="ACHA6B_000443"
     CDS             9749..10621
                     /locus_tag="ACHA6B_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948047.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11079..13277)
                     /locus_tag="ACHA6B_000444"
     CDS             complement(11079..13277)
                     /locus_tag="ACHA6B_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948048.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13509..14501
                     /locus_tag="ACHA6B_000445"
     CDS             13509..14501
                     /locus_tag="ACHA6B_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8948049.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14561..15982
                     /locus_tag="ACHA6B_000446"
     CDS             14561..15982
                     /locus_tag="ACHA6B_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8948050.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15975..17474
                     /locus_tag="ACHA6B_000447"
     CDS             15975..17474
                     /locus_tag="ACHA6B_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8948051.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17513..18907
                     /gene="uxaC"
                     /locus_tag="ACHA6B_000448"
     CDS             17513..18907
                     /gene="uxaC"
                     /locus_tag="ACHA6B_000448"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8948052.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19066..19749
                     /locus_tag="ACHA6B_000449"
     CDS             19066..19749
                     /locus_tag="ACHA6B_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8948053.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19751..20587
                     /locus_tag="ACHA6B_000450"
     CDS             19751..20587
                     /locus_tag="ACHA6B_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8948054.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20598..22352
                     /locus_tag="ACHA6B_000451"
     CDS             20598..22352
                     /locus_tag="ACHA6B_000451"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948055.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22628..22969)
                     /locus_tag="ACHA6B_000452"
     CDS             complement(22628..22969)
                     /locus_tag="ACHA6B_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8948056.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23037..25208)
                     /locus_tag="ACHA6B_000453"
     CDS             complement(23037..25208)
                     /locus_tag="ACHA6B_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8948057.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25419..26276
                     /locus_tag="ACHA6B_000454"
     CDS             25419..26276
                     /locus_tag="ACHA6B_000454"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8948058.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26347..27204)
                     /locus_tag="ACHA6B_000455"
     CDS             complement(26347..27204)
                     /locus_tag="ACHA6B_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8948059.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27189..29891)
                     /locus_tag="ACHA6B_000456"
     CDS             complement(27189..29891)
                     /locus_tag="ACHA6B_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8948060.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29904..31193)
                     /locus_tag="ACHA6B_000457"
     CDS             complement(29904..31193)
                     /locus_tag="ACHA6B_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8948061.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31343..32389
                     /locus_tag="ACHA6B_000458"
     CDS             31343..32389
                     /locus_tag="ACHA6B_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8948062.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32391..34544
                     /locus_tag="ACHA6B_000459"
     CDS             32391..34544
                     /locus_tag="ACHA6B_000459"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8948063.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35017..36468)
                     /locus_tag="ACHA6B_000460"
     CDS             complement(35017..36468)
                     /locus_tag="ACHA6B_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8948064.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36465..38189)
                     /locus_tag="ACHA6B_000461"
     CDS             complement(36465..38189)
                     /locus_tag="ACHA6B_000461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8948065.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38182..38796)
                     /locus_tag="ACHA6B_000462"
     CDS             complement(38182..38796)
                     /locus_tag="ACHA6B_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8948066.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39141..40598)
                     /locus_tag="ACHA6B_000463"
     CDS             complement(39141..40598)
                     /locus_tag="ACHA6B_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8948067.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40639..41559)
                     /locus_tag="ACHA6B_000464"
     CDS             complement(40639..41559)
                     /locus_tag="ACHA6B_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948068.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41571..42518)
                     /locus_tag="ACHA6B_000465"
     CDS             complement(41571..42518)
                     /locus_tag="ACHA6B_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948069.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(42997..43716)
                     /locus_tag="ACHA6B_000466"
     CDS             complement(42997..43716)
                     /locus_tag="ACHA6B_000466"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8948070.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43765..45411)
                     /locus_tag="ACHA6B_000467"
     CDS             complement(43765..45411)
                     /locus_tag="ACHA6B_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8948071.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45590..46180
                     /locus_tag="ACHA6B_000468"
     CDS             45590..46180
                     /locus_tag="ACHA6B_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8948072.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46272..47777
                     /locus_tag="ACHA6B_000469"
     CDS             46272..47777
                     /locus_tag="ACHA6B_000469"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8948073.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(47861..49213)
                     /gene="celB"
                     /locus_tag="ACHA6B_000470"
     CDS             complement(47861..49213)
                     /gene="celB"
                     /locus_tag="ACHA6B_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8948074.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49213..49731)
                     /locus_tag="ACHA6B_000471"
     CDS             complement(49213..49731)
                     /locus_tag="ACHA6B_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948075.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49747..50073)
                     /locus_tag="ACHA6B_000472"
     CDS             complement(49747..50073)
                     /locus_tag="ACHA6B_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8948076.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50086..52065)
                     /locus_tag="ACHA6B_000473"
     CDS             complement(50086..52065)
                     /locus_tag="ACHA6B_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8948077.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52086..52400)
                     /locus_tag="ACHA6B_000474"
     CDS             complement(52086..52400)
                     /locus_tag="ACHA6B_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8948078.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52568..52861
                     /locus_tag="ACHA6B_000475"
     CDS             52568..52861
                     /locus_tag="ACHA6B_000475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8948079.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(52939..54051)
                     /locus_tag="ACHA6B_000476"
     CDS             complement(52939..54051)
                     /locus_tag="ACHA6B_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8948080.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54204..55052
                     /locus_tag="ACHA6B_000477"
                     /pseudo
     CDS             54204..55052
                     /locus_tag="ACHA6B_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55405..>56243)
                     /locus_tag="ACHA6B_000478"
     CDS             complement(55405..>56243)
                     /locus_tag="ACHA6B_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8948081.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        16756 a        11508 c         9915 g        18064 t
ORIGIN      
        1 ttaaattaca gatgatcttt tccatcatac ttgaaaaact caaaatcagc ataacttcct
       61 ctttttttat ttagatcatg cactgctatc ccgataaaat ttccagtaaa ccctcctgag
      121 agtatacaca tgtttttttg cggacctact ttcttccatt cttcagttga tacgtctttc
      181 aaatgaaact gtgctgtctc tttctttaca gtgactttca aaaaagtctt cttttctgtg
      241 agtggaatcg tccaatcttc tatttgtgct actccgtttt ctgattctat catacgtagg
      301 cattttccct tgtcctcatt ccaagtaaca taacaataaa tataattgga ttcatttaag
      361 tacagtacca gtccagccat ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc
      421 gcatcaaaag taaaatccgt ttgccgaata gctaacaaat gatgttcgaa ggtagattga
      481 agagattcac cggaaatcag acgtaaaaaa cctggtctac attttgtatc acaccaatca
      541 gcatttggca aaatgcgtct ggcattccag ttcttcttta gctctccttc aaatgtatcc
      601 ataaactgat gatctttttc ttgttgtacc tcttcatttg tctcaatcat tatttcttcc
      661 atcggagttg tctggctatc tttaagtctt aaccaatcgt cggacgtcca atacacttct
      721 tgaatagcgg tttctcttcc caaaatagcc gcaccgttta atggtctggt acaaagatat
      781 gtcatgtacc atcttcctga cggggtttgt actaagctcc catgtcctgt acattgcaat
      841 gtagaagcag gtttgtcact agctgtcagc ataggaaaac caggatcaag ctcgtaagga
      901 ccagtaatat tctttgctcg gcaaactgta actgaatgtc ctcttcctgt accgccttct
      961 gccgtcatca aataataata cccgttacga aaatacaaat gtggtgcctc cgttttcgct
     1021 aattctgtac ctgcaaatat tttatagatg ggaccatcaa gcttcttctt ctctgcatca
     1081 tattcttgta atacgatacc gctggattta ttcggtgtgt ccagacgata atcccatagt
     1141 tcattgacaa gccatttttt tcccgtttca agatcatgga acaaagaagg atcaaagccg
     1201 ttactattta aataaattgg ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa
     1261 taattgtgga catctttaaa cggacggcct gcacttttga catctgtgta aacaagataa
     1321 aacaatccat cagcataact gatctgtggc gcccatatac ttccatttgt aggatttcct
     1381 tgcaaagata cttgagtggt caaaatatcc gttgtatgtg accagttgac taaatttttc
     1441 gactcatata ctcggattcc tggaagccat tcaaaagagg agacaataat gtaataccgc
     1501 tctttcactc ttaatatatt aggatctgga ttgaatcctc ttaaaatagg atttttcact
     1561 ttgatagtct gcatgattca ggctcctttt ctcattctta ctcgatataa actgaatgcc
     1621 ccagctgcga aataaaaaaa tgcaaaagga aacgcaataa actgagtgcc aatcaacaat
     1681 atgaatcctg ttataaaata caaaccagtg gcagttgtta agtattttcc tttagcagtc
     1741 aaaaacaatg ctactgctaa aaaagcaagt gaaataaaca acacaacggc aaacagacca
     1801 gtcatgtcct gtaataatcc aaatgtattt tctaaagaca aagttgaatg atctacagca
     1861 agcgactgat aacttttttt aaactctgac gcagacatat tacttaaagt aactgctaac
     1921 ccgccaaaga aaatcaaaga tagactgcca ccgaccattc ctagcaatag ttcccatttt
     1981 cttttcattt ctgaaccccc tgcttttcta aatgtatcca ttcaggtctt ttgatcgatg
     2041 tttcataatt gttattacgt tcagagaatt tgacagtctg ttcatttttc ttgtcatccc
     2101 aattatccca accagttggg tgaacatgcc cacctatctt acacgcttca aataagacat
     2161 ttgcatacgg acgccacggt cttcctaaat agacgtttct agtatcaggt tctgcagtca
     2221 aggtacagct ttcaaaacga taccctgttt tttgattttt cggcgtagat gctgctgtga
     2281 tgtatccagg atttttactg gctaagctat ggatatgaca ctgatgaaaa acggcatctg
     2341 ccccaccaaa tatgaaatcc accgttcctt caatccagca atattcatag gtctgacgac
     2401 aatactcagg attggcagaa gccggtgtac aaaaaggagt accatctttt tgcaaatcag
     2461 gcaacggacc cgtgcataag gtatcctgat aaccagatag tcgacagttt tttaaagata
     2521 cgcgatctcc tgaattgaat aaagcaacag cctgtcctac cttttctcct ggacctgctg
     2581 tattttcgat gtgaagattt tctaatacga cattcgatcc ttctaagaaa aaagtagcac
     2641 ttcggaaagt ccctaactgg ctaccgtcaa gatactcctg ttttgcaaaa cgattaccgg
     2701 agataactac tgttcctgct cccacaagtt taaaattgga taatctgctt tctatatatt
     2761 catcatatct tccttctttt actatcaaga cctttggatg gctatccggc aggcctgcca
     2821 aataatcgat tccttcttgg atagtttcaa aatccgtcgg ggtatctata gcattctttc
     2881 caattatcag tttgtctttc atcctcataa atgctccctt atgaattgtt tccaaatatt
     2941 tctcatttct tgtgtttcca tatgaccgcc tgttaaagaa aaagaagaaa agttttctgg
     3001 actacccatt gattgatact ttttgtccag cccttttctc agcttcagta cgccttcgtc
     3061 caagcacatc gtatcgtttt ttccaactaa gctaagacga gggcgcggag cgattttttc
     3121 ttggattgct aacgttgtat aagctgtcaa tagatttggc acatagtaat aaaacccatg
     3181 atgatctagg cggcgatgta gcaaaagtat ttcgatgtcc acctgtgccg ctagatcaat
     3241 acaaactttg acacgagaat ccaatgctgt cacccaccag cttaataagc ctcccataga
     3301 aagaccaatc gttgcgagcc gttgagtatc tgtgtcttcc cttgtctcca aataatcgat
     3361 caagctgatc acatcataaa tacgcattcc ccataacgtt tttcctgtta ataaaaattg
     3421 tttgaacaac tcgctttcag aaatacctcc gcgttcttca aatccccaag catcaatcga
     3481 ccaaacagca tatcccattc ctgtcagtac ttctgcaaaa gatggagaag ctaagtaagg
     3541 agcaccatgg atcaattcac ttttcccgac tgaaaaatct ccgccgtgag aatgcaggta
     3601 aaggaccggc ggaaacggtc cttctttttc tggataagca tagagggctg gtactgtttc
     3661 caattcattt aagtacaatt gatagtattc aagcataaaa ccgttttgtt tttctcggta
     3721 taatcgtttt ccactagggg tatgtctgct agatggagca cctaaaaaat cattcaactg
     3781 cataagcaaa gtttttacga acaaccagtt catccatatt ttctgattga tttgattgga
     3841 atttctcaat actgatttct ttagtatttt ctaatagaaa tgctggtccc tcgtgatttt
     3901 caatcgacat ctggttaaag gaaaccttac ccgcaaagcc aatataaaat ccttgtctgc
     3961 tcatttcctc tattcctgtc atcatagcag gaacaccggg aactgcatct tgggccatcg
     4021 agacatcgat attgtcaaat gtgatttctt gtatgaattg ttccgctaat ccataaataa
     4081 aacctgcaga agcatggaca ttccttgccg tgatattcga aaaatggatc cttctgaatg
     4141 ctggtgtccg ttcatctatc ggataagcct ttttttccca tacatacggt tctttccctc
     4201 gcggaccaca gaaataataa agattcagta taaatggaca catcacatta tccatgacta
     4261 tattggatac tcgaatatct tcaacgatcc cgcctcgacc tcgtctagat ttcaatcgta
     4321 ttcctcgatc agtttcttgg aagatacaat ttgaaatggt aatattacga atactaccac
     4381 tcatttcact tcctaatacg acaccgccat gtccatggac catcgtgcaa tttgtgatcg
     4441 tgatattttc gcaagcgatc ctttcgtacg tatcttcagt ccctgccttg attgcaatac
     4501 agtcatctcc cacgtcaata tggcaattac taatacgcac atttttgcat gattctggat
     4561 caattccgtc tgtatttggt gaatccgctg ggttcaagat cgtcaagtta tcaaacgttg
     4621 cattactgca aagaatcggg ttgatcgtcc aacttggtga ctggatcaat ttaatatctt
     4681 tcacagtaat tcgatgacag ttatggaaac tcatcaattt aggacgagga taagcgagat
     4741 tatctggttc atttctaaaa gtatgccacc atttttttcc atttccatct aatgtaccga
     4801 aaccagtgac agagatgttc tctacatttt gagcatagat acaggaagca taaacttttc
     4861 gatggactcc ttcccaacgt gaatgaacaa caggataatc ttttggatcg tcagagaatt
     4921 tcagaaccgc tcctgcgctt aaatgaagtt cgacattgct ttttaaaaat aaagctcctg
     4981 ttagaaattc acctgctgga acaactacgg tcccgcctcc gtcacttgct gcagcatcaa
     5041 tggcttgttg tattgcttca gtattcaatt catcaattga tgcgccaaat gtcaaaatat
     5101 catacatatt tataatgcct ccacttcaat caatgcttgc aagaatgcac caacgccttt
     5161 ttgatcatta caaataattg gttcactgat gtagtaagca tatgaaccat ctcgctgatc
     5221 tgctccgcct aaccctgcta cttgacagtt tttattcaag ttcagccagc cttcttttgt
     5281 ttccagaaca aattcatcaa gtaatccttg atgtgatttc ttcacttgtg gaagccattc
     5341 ttctttgttc agcagtccca aacgtattcc tttagccatt gcgtaaacaa acatactact
     5401 gccggaagct tccagataat ttcctttccg ttttgtttct gttgttactt gataccatgt
     5461 cccgctgttt tcttcttgat atggaagtaa tgcagccagt gtcttttcta gaatttcttt
     5521 cagcaaagaa caatctgttt cttcatgaag taattcatag gtatctgctg cagccatcaa
     5581 ataccagcca atcgatcggc tccaaaaatg atgggataga cctgtttttg gatcagccca
     5641 tggttggacc ttcttttcat cccatgcatg aatcagcaaa tttgtttttg gatcgaatag
     5701 atgtttataa ctgatttcaa actgtctcaa aacatcttgg taatcttttt cttcactaaa
     5761 attctggata aattcagcat aaaaaggaga acccatatat aagccatcca accaaatttg
     5821 ataaggataa atttctttat gccaaaaagc tccttcggat gtgcgtggat ggtttgccaa
     5881 ctgctgggaa aggagttctg ctgctttttt atatttttct tcgcctgttt ctttgtacaa
     5941 taaaaagaaa agctttcctg tatttacatg gtcgatattg tattcttcaa cgctgtagcc
     6001 acggatcgtt ccatcttctt gcacgaaata gtcgatattg tcttttatga aatcaaaata
     6061 aattttttct tgggttcgtt cccaaagtaa ttggaaccct ttaagtacga cgccatagtc
     6121 ataagaccat tttcctttat accacttttc ttcatataag cgaggtgttc tttccatgat
     6181 actttttgct aatttctttg accagttttc cattatttgc tctcctttaa aaattaaaat
     6241 aggatttggg acaaatacca agaagtattt gtcccaatcg tctatattac tgtgccgctg
     6301 ctttgtatgc ttcctcgtac tcttgtgtga ttcgatcgcc tccttgtgct ttccagtctt
     6361 ctacagcttt tttgaatcca tcttcgtcaa gctgtcccat gatgtattgc accgtagcat
     6421 caatgacgat tttttcaatt tctgatccta cttcattgtt cgtcggtgaa tccaatggca
     6481 cagaaggatc cattacagca aattcttcat tttctttgat caattgatta gagaattctt
     6541 tttcagggtc tgtatctttg aaagagaacg tcacttcact aggacgagag cttgataacg
     6601 gttgcacctc ttgttgccaa agatccatat cgataaattc gatagaacca tcatcattca
     6661 attcataatg cgttccttcg ataccgccag tcatcaattt atacacatct tcttctaaaa
     6721 gatcatcaac aaattgaagt aatttcttca attgtgcttc gtcttttact tgagatttgg
     6781 gaaatgctag caagccgcca ataccatttc cttctgacca aacatgataa ccgccgcctt
     6841 caccatctga gatcttgttt acaggcacta attccatatc atcttgaata ccttgaccca
     6901 tcgtcttcag attcttgata tccaccattc cagtgtagat tccagttctt ccttgtgcaa
     6961 attgttcttg ctgatcagtc ttttgggtaa ctgcaaagtc ttgtgccaaa tagccattct
     7021 caaatagatc tcttgaaaaa ttcaaagctt tcatatactc ttctgtatca aattctggag
     7081 tgaatttgcc attatcgtct acagcccagc catttggtgc tccaaaataa gaagtgaaaa
     7141 gtttaaagct ggtataacga atatcactag gcgcacgatc gccaaaacca actgtgtcgt
     7201 ccacaccatt tccatcagga tcgttttctg taaattgttt agctacttca taaagctcat
     7261 ccatggtagt tggaacatct agtcctagat tatccaacca gtcttttcga atgactaagc
     7321 cagcacgagc gtagtctttt tgtatcggta cgccgtacaa ggcaccgtcg atactcgcag
     7381 cttcgattct atcttcagaa atttgactta aatttttata gtcttttaaa tattctgata
     7441 catcccagaa tacgcctgat tttaacgcat tgcggacaga agagtttttc aacatcgcaa
     7501 tttgaagtgt aacgatatct cctagttcat cagaagccaa tgcagttgtt aatctttctt
     7561 cttttgaagc atcaggaatc cagttgaatg tcagttccgt atcggttttt tcttcgatca
     7621 agttcacaat cgtgtctgta ggcggtgaag catgatgcaa aatatttaac caagtgattt
     7681 tagcttttcc atcttctgtc tctgaagaac tgttacttga actgctgccg catgcggcaa
     7741 gtgctagaat tcctgcaaaa ctaagaccta attttacgag cgttgctttt ttcataaatt
     7801 ttctccttca ctgttttaat aatatttttg ttttcttctg ttttttctgt atatgagttc
     7861 ttccattcca aatggcaaaa tatagaattt gctgtccacg tgactgcgga aaagaagaga
     7921 ctgccaccga taaaggttaa aacacctggc attgtttgga tgatcatcag ataaatccct
     7981 accatactta atatcaacac taatgagtaa tgaagatgtg agaatccgaa taaaaatgcc
     8041 attttcattt tgaaaaaata tcctttccaa tcataatgag ccatgattgg gaaaatatag
     8101 agtgctgaga gcacatatat gaacgcagca atatacaaag cgatcctaag ataccaattg
     8161 tgtatgaaag aaagatccac aagtaaaaca atcccgatgg cgccatatat ccagctcatc
     8221 agcaaggttt cacgatagtt ttctttaaaa tatgtgaagt acgttttcgt caaaggaaac
     8281 gtttcttttg agcgcagcca ttttctaatc actgcaacca tcgcatacga tgcaggaccg
     8341 aaagtaaaaa ctcccaaacc tgcgatgact aaaccgcccc acagaaaatt caaataaacc
     8401 aactgtaaaa tacgatttag ccttccgttt attttatcta atgactgcag catatctttc
     8461 cctctctttc tatcttagta aacgccgtct tctcctagct ttttggcaat cttattagct
     8521 aaaattacca taaacaatcc tacgacccct ttaaacaatc ccattgctgt actgaaactt
     8581 aactgtccat tcttcaatcc agctgtataa atataggtgt cgaaaatttc tgcaacgctt
     8641 cgattcaatg aattcaataa caagtacata tgctcgaacc ctaagtctaa tgtctgaccg
     8701 atttttagaa tcagcaaggt aatgattact ggacgaattg ccggcaatgt aacatgccat
     8761 gttttgcgta agcgtcctgc tccatccatt tctgcagctt catacaactg tgtatcgact
     8821 gcagttatcg ctgaaaggta gatgatcgta gaccagccca actctttcca gatcacttgt
     8881 ccgatatata ctgtgcgtgt ccattcaggg gatgttagaa aactgatttt ttcaaatccc
     8941 attgttgcaa ggaattcatt caatacccct ccgtcaacat tcaaaaatac ataagtaatc
     9001 gaaacaatga ttacccaaga catgaagtga gggatgtaaa tgatcgtttg aatcccgttt
     9061 ttcaatcgtt tgttcgttac ttcatttaat agtaaagaca aaatgattgg catcggaaaa
     9121 aacacgaaga tatttaatcc gaacaagatc aacgtatttc ttaataatgt aaagaatgtc
     9181 ggttctgtaa acaaacgaat gaaatgtttg aaaccgaccc aaggacttcc cattattcct
     9241 aaaaatggtt gataatcttg aaaagcaata acgagtcccc ccataggaag atacctaaaa
     9301 atcaagaaat aaaggactcc cggcagaatc atcacatata aaaatttatt cgttttgatt
     9361 cctgcaatta ttttttttcg tcttctgagc ttttcttctt ttttcgatag cgtcagttga
     9421 aatggttctt gtttctttac actttccata agcccctcct tagttttgtg aatgtttgct
     9481 gcttatgact tcatgataga taactatctg aattacgtat ataatcattt ttaccgtaag
     9541 taaaaacgct tacatagcaa tgatttttgt gctatacaaa acgataaagt gtattttttc
     9601 acaaataaac tacgcatagc aaaccttcta tttttttcta tctgacactt agcataggaa
     9661 atgattatat acgtaatctt tcaccgatga taaattgaac atatagaaac agaaacattt
     9721 aatttatttt gaggaggagc ctatctatat gaaaaagaaa gtctctatag gagaaagaag
     9781 ttttacactt ttcaacaata cgtttctaat tttgctagct ttgatctgta tcgttccttt
     9841 tttaaatatc atcgctacat cttttgcttc tacacaagaa gtcgtcgcga aaaagtttat
     9901 cctttttcct actacctttt ctttagacgc gtatcgctac attctttcta cgccgaccat
     9961 ttttagagca cttgctgttt cgattggtgt tacaggtgtc ggaacgatcg tcagtatgtg
    10021 tgcaacttca ctgatggcct acggtctttc cagaaagtat ttgtttggtc gaggtttcgt
    10081 caactttctt gttgtatttt ctatgttgtt cagcgggggg atgattccaa cgttcttagt
    10141 tgttcgttct ctagggctgg tcaattctta ctgggccatg atcctgcctg tggctgttaa
    10201 tgcaatgaat atgatcatta tgagaaactt tttccaagcc ttgccggata gtttggaaga
    10261 atcagcaaaa atggatggtt gcacagattt tggtgtattt ttcaaaatta tgctgccatt
    10321 agcgttaccg tctattgcta ctatctcgct tttttatgct gttacttact ggaacactta
    10381 tatgactgct attttgtata tcaacgattc atctaaatgg ccgatacaga ttcttttacg
    10441 ccaaatcgtc attgtatcaa gtgggatgca agcagaaagt agtgccgttg atgtgattcc
    10501 accagcacaa acagttaaaa tggctgttat cgttattgca acagtgccta tgctgattgt
    10561 ttatcctttt gttcagaaat attttgtcaa aggagcactc gtaggttctg tcaaagggtg
    10621 attatgtact gaacgcaaaa tggtagttgt gtcagaagtg gtcagcttca agaaataaga
    10681 aggcatttac gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tttcgaagaa
    10741 tctactcctg gaacaccgtg tatcagaggt tgtgaaagag acgttcagcc ttgagcgatg
    10801 ataaaaactc tcacaaacat tgcgacgagt aacccacagg agcaataaga agcgattttg
    10861 aaaaatagct tcccatattt cacaaaatta tgccttattg tcgatagcct gtctcttgaa
    10921 cgccatttat tcgtaaaaaa gaactgtgac aagacttttg tcacagttct ttttgcgtgc
    10981 ttagccttga aatttaacta tccataaata caaatactag ggttattcta acttctttta
    11041 ttgcttgaat cagtccacaa gaaaaacagc ctcttgattc acaaatattt ttttcgatat
    11101 tctcccggcg taatcgattc cttcttttta aagaaacgaa taaaattttg cggatttcga
    11161 tattgcaatc tttctgcaat ctcttttacg gagaggttag tctctttcaa ccattttttt
    11221 gctacctcta aacgatgatt ttgaacaaaa tcaccaaatt tttctcctgt ttccttttta
    11281 aatacattac ttaaatagtt agggttataa tgcaacctat cagcgatcac atctaaagac
    11341 agatcacgat catattctgt gtagacgata tgcataatat tctccgacaa atttttaaat
    11401 tcatgatcag tgattgcttg agagcattct gtgattggca gaactaattg ttctaaaaca
    11461 agtttttcta gttctttggg atgataattg ttcagtgctt ttacataaat ttgtttcatg
    11521 ttatcaaagt tttttgaatc tactcccaat aactgtccta attgaacgag ttcattgatc
    11581 aagcggaccg aaactacttc ccgactcaaa ggatttttat tttgtgcaaa caatgcatca
    11641 atcaattcat gtactaaatt cttcacttcc tgatttccag aacgaatggc ttcaaacaat
    11701 cgattttgtt cttctatcgg atatctaata agtgtttttt catgagcagc aggaacgatt
    11761 tcctgataaa aaataattga acttggacca gtatttacac gataatacag ggcttctttt
    11821 gcccgatcaa ccgattgctt gctttcattc agcgtttcga accggttact gatgcctata
    11881 ctgatagtta aattcaagta ttcttttaca cgtttttgaa tgagtcggca atactccatg
    11941 acttttttgc ttgcttctgc atcatcttta tccattcgat aaatagtggc ttgcatttct
    12001 tcattcaaaa cgatcggcag cattcgattt tcctttggca caatttccgt gatcatgttg
    12061 ttgatcgcta aaagtagcaa atcacgctcc ccaccgtgac tatcttctaa cagatcgatt
    12121 tgaaccaaag ctgtataata gcagtcattt tgagaactat aaccaaattg ctgcaaccgc
    12181 tgatctagtt ctcgcctttc tactcgattt ctaaataaac taagtacaaa tagcgtttcc
    12241 agctgtggct tttgggtaac taagtgtgca cttaacgctt ctttttctcc tatgatccga
    12301 ctgactgatt gagcgaccaa atctagttct ttcccactga atccttttga tttaagattc
    12361 agacgttcct gtatttgaga aatcggacgg gcaaatctgt ccgaaaataa gtaggaaaga
    12421 agaccaatga gtcctaccag tgttaaagac actgcaacta gcccgattct cagattgcgg
    12481 atcattgcgc caattgcact agagtcaact tctaatctgt aaatccaatt attataatct
    12541 gatttcagta ctatatacgt tttatctttt tctcttatca ctttcagctg attgtaatct
    12601 agatccacgt ccgttaatac agttggttgc tctttatttt ttactgtctg agtagtaaaa
    12661 aggttgccaa cttcgttttc aatagaaagt atgtcttctc cactttcacc aacaacttgt
    12721 tctattgtat gtttctctat cgtagcaatt cccaatgcat atttttcttt ttcaaacatt
    12781 ggcaaagaaa tcaccatttc gattcctttg tttttctttt gccaaaataa attatttttg
    12841 ttatttatgt acttctgttg atacttttca atttcctctt cggtcagttg actcaacgat
    12901 ccattgatta ctccccattt accagccaaa ctgactaaat cataattatt atcatccata
    12961 acaatcgttt ctataaaatt tagttctttc ctgatttcag aataagtgga gtaatctgta
    13021 tatgtcagcg gatgcttgaa tatgtccgag aaatttttgg tcgaaccata cgtagaaaac
    13081 gaatattcaa tcgttctaat tttccattca acgttagttt taacttgcct gagcaaattc
    13141 tttttgtctg ttaaataact cgattcgatc gattcatagg tattctggta aatgaataaa
    13201 ctaaatccta tgacaagtac ggctcctaat aaaaacatcg gtaaaaatat tttataaaat
    13261 actgtacttt tcttcatttt cttcctctcc tccttttaaa ataaaaggct ttcatttttc
    13321 tgtaaaattg atactcatct aattaaatga gcattccccg ctttcaagaa tagcataatt
    13381 aaaaataaaa atccaagtaa tttttaacgt gtgcaatctt ttcatatcaa agatctattg
    13441 acttatcgta gttatcgtaa tatgatgaat gcgttaacaa aaagtgaaaa agtaaggaaa
    13501 atgatggaat ggtaacgatc aaagatgtcg ctaaagcagc tggcgtttct catacgacag
    13561 tttctcgtgc tttgaataac agcagtttaa taaaaaatga aacaaaacaa aaaatacgac
    13621 aactagctaa ggaaatgaat tattcgccaa actatagtgc aaaaggttta gtgaatcaaa
    13681 aaaaatatct aatcggacta tttttctcaa gtttgcataa aggaacttca tcaaatttcc
    13741 tagcagattc cattgcaggc atccattcag tattagacac aaattacagt ttatctgttg
    13801 aaagtatcga tgttgttgat ttaacagaaa tcaacctgca acgctacgat ggactgatcg
    13861 ttatgagtca aagtgatgat gatcagcctt tcatcgacta tctcaaagaa aaagaatttc
    13921 ctttcgttgt tgtcaatcgt cacatcaatg atccagcggt tatcaacgtc actgccgatg
    13981 atgcggccgg tgttatgcag gcagtagatt acggtattgg atgcggacat acgaagatcg
    14041 cttatattgg cggaaagaaa aattttcgtt cgacaaaaga acgggaaaaa ggggtattga
    14101 aaagtctaaa aaaagcgagc atccctgtca atccagccta ttttttaaca ggagattaca
    14161 gcttagaaag cggcctccaa aacatggaat acctattatc actttctgac ctgcctactc
    14221 tggttttttg cggcaacgac gacatagcga ttggtgcatt gagagctgta catttagctg
    14281 gcctccgtgt tccccagaac atttctctga tcgggttcga tgattctcca gtcgtctcct
    14341 atctcaatcc gccgcttacc accgtacgta agcctttgaa agaaatgtgt caagaaggaa
    14401 caaagctttt actagatttg atcgaaggca ggacgatcga acaatctaaa attgagctct
    14461 ccaccttgtt gatccttcgt gagtcgatca attacttgta aactacaagt aattttatat
    14521 aaaaatgtta acgtgtgcat aacacagaaa ggaagtttat atgaaaaaac tgacaaaaga
    14581 attagtgaaa aaaaacgaag cacctgtaaa agttcttcag tttggtgaag gcaattttat
    14641 gcgtggattt attgattggc agattcagca gctgaataac caacaattgt ttaaaggtaa
    14701 tgttgcaatc attcaacctt tagaacaagg tcttggaaat atgatgaacc agcaagataa
    14761 tttatatacg gtcattttac aaggactcag caatggcgaa atcgttgata cttccgagat
    14821 tatcaccgta gtagaacaaa tgatcaatcc ttatgatgaa tgggatgctt atctatctct
    14881 agcagaaaat gacgacttgg cctatatcgt ctccaataca acagaggcag gaatccaatt
    14941 cgtggagact gataatttaa cagatactcc cccacagagc ctcccgggca aactgacagc
    15001 ctttttatac cgacgtttca aattaaacaa agctggcttt accatcattc cttgcgaact
    15061 gattgacaga aacggcgaac gtctaaaaga aatcatttta cgctacgcag aaaaatggga
    15121 tttagaacct gcttttgttg agtggttaga aaaagaaaat gttttctgtt gtagtttagt
    15181 tgatcgcatc gtcccaggtt atcctcgcga agatgcaggt gttttcaatc aacggcataa
    15241 ctatgaagat caattgatgg tcaaagcaga accattcatg ctttgggtga tcgaagggcc
    15301 taaagaattg gagaatcaac taccattcaa acaggctggc ttgaatgtca tcatcacaga
    15361 tgatatgact ccttatcgtg aacgtaaggt gcatttgctt aatggaccgc atacagcgat
    15421 ggtccctctt gctttgttgg cggaattgga aactgtagaa gaagtaatga aagatccttt
    15481 attctccgca tatgttgatc agttattcaa tctagaattg attcctatgc tttctttacc
    15541 taaagatgaa ttagccattt atgctgacca aatcaaagaa cgtttcttga atccttttgc
    15601 ccatcacaaa ttagaagcga tttcattaaa tagtgtttca aagttcagca cccgtctgct
    15661 tccagtgttc aagaagtata tcgaagaaca aaatcaggta ccaccgttaa tcactgtttc
    15721 actagctgcg ctacttctta tgtatcgagg cgatcaagta aagccacatg atgatgaaaa
    15781 gacgataagc gaatttactg atgcttggtc tgataatgga acagctattc ctcgattatt
    15841 gcaaaacgct gcgctttggg gagaagattt gtctcaaata ccgaatgtga cagatacagt
    15901 tcaagaaata gctgcaaaga tcgatcaaaa aggtgtccgg tcagtattac aagaaatgaa
    15961 aggaacgatg gtccatgctt gaaaccctgc aaccaacaat gaaattaaat cgacgagaca
    16021 gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt gatcagcaaa
    16081 cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt gatttgaaag
    16141 ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca gacgtaactg
    16201 ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa ttggattatc
    16261 gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca tttcaaggtt
    16321 acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta cctaccgttg
    16381 ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa catccagatc
    16441 tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg cagcttggaa
    16501 aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc aatgccggag
    16561 gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc aaaaaaattt
    16621 taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt tacgatgaga
    16681 tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat gaccagcgca
    16741 tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct gatggtctat
    16801 cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt gctcaaggag
    16861 gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta ttgatggcac
    16921 gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac ttcaaacagt
    16981 atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat aaagcaggtg
    17041 ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga agctctcctg
    17101 tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt ttattgcaag
    17161 ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc cagttggtct
    17221 tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc aaggtatcta
    17281 cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca ggcgtcttat
    17341 tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc gctgtagcta
    17401 gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt tttaaaaacg
    17461 gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat ttatgttttt
    17521 atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt cctacgcaaa
    17581 aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt acgaaaacaa
    17641 caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat ggcgcttgat
    17701 gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt ttgaaaaatt
    17761 tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca gttggtcaca
    17821 tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa atgcagagaa
    17881 aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc gggaaatcgc
    17941 aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg acttgcacta
    18001 tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat tccgtccaga
    18061 taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac tggaagaagc
    18121 agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc agcggataga
    18181 atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct tacgctataa
    18241 agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta acaaactatt
    18301 atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga cccacttcta
    18361 tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact gtaatacaca
    18421 aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc tttctttgac
    18481 tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc caaagacgat
    18541 cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg gctgtttcca
    18601 aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg atactcgagc
    18661 tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga attttgtcgg
    18721 catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt tccgtcgtgt
    18781 tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag atgaacaact
    18841 tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact ttggcttttt
    18901 tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa ataacggaat
    18961 ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt cttctcttgc
    19021 tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc tactatccat
    19081 attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga gacaggttgg
    19141 ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca agcaaagaat
    19201 ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc ttcacagttt
    19261 cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat ccaagaagaa
    19321 agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt tgaatgcgca
    19381 aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga ttacttagaa
    19441 aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg tgcatttgct
    19501 gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt gttccagacc
    19561 ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac ttgtaaaaat
    19621 tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga taaagttgcc
    19681 cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta tttacaaaaa
    19741 ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag atgttcgaaa
    19801 tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc aatttgcacc
    19861 acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga gtgctgccta
    19921 ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat gctatatcaa
    19981 cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca aacagcaaat
    20041 tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta gtgtctctgt
    20101 cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa attctatcat
    20161 tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag cagggatcaa
    20221 tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag cttcaccaaa
    20281 tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg ctgatcagga
    20341 aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc ttcatctcaa
    20401 agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg gctgtttaga
    20461 tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg ccactcttga
    20521 agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta tttaccaaga
    20581 ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa tatcatgcaa
    20641 atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt gccatgtcag
    20701 gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct attgccaatg
    20761 taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat ttcaacgctt
    20821 ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat gtaaaatacc
    20881 atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc aagaaaatcc
    20941 aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag tcaaagatca
    21001 tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc ggattaaata
    21061 tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct gttgttttca
    21121 tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc cgtaaaagga
    21181 tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca aaagtgatcg
    21241 aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac gaaacaactc
    21301 gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat atcctgcaat
    21361 ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt tgtttgattt
    21421 tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg atgtatcaga
    21481 cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt cctacgattt
    21541 caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc gatgtagaaa
    21601 cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt gaggatgttt
    21661 cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta gaggtgaaag
    21721 ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca ctccttaact
    21781 tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat cagcctttgg
    21841 atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa accactttgt
    21901 tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta tcaaaagaac
    21961 gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag cttcctgatg
    22021 gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa aagcaacgta
    22081 tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat gaagcaacct
    22141 cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac ctgacagaaa
    22201 cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca gataaaatcg
    22261 ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg ttagctcaaa
    22321 aaggctattt ctaccgcttg aaaaatgaat gacaaaaaat aggagctatg acaagacttt
    22381 tgtcgtagct cctatttctg aataaacggt gttctcctgc caactcctcg gtcacaagtc
    22441 acaatagtca tcaaacatga gaagctgttt tttgaaattg ttccttgtaa ctcagagtta
    22501 aacggtaggc tcacgacctc ttggtaaatg gtgttcaaga gacagcctat cggcaataag
    22561 aagcaatttt gtcatcgctc aaggccgaac gtctctttca caccctctgt tgtttcataa
    22621 aatagtttta ttcgctgtaa aggtcgcgaa ctggtgtcat gatgatacgg ttcaagtcat
    22681 tgatgattgt actgaagcct tgctcttttt gcatcaagtc attgattact tcttctgatt
    22741 ggattttcac agccatttct tgtgctttca ttgcatcttc gtcgctgaac tcttgtccac
    22801 tcatttgttt ttcttgtaat tcgatttgca tcgcttggaa gtctttgaac agtttgtgtg
    22861 cttcttcgtt tgcttgtact ttctcgtacg cttctttcaa cgctttgaat tctggtaatt
    22921 cacggatctc acgttcgatt tgattcgcgc tatcgtagat gtttgacatg ttatgccctc
    22981 ttttctttta gtagttactc tctattgtaa catggggtta ttcgttttca aaggggctac
    23041 tctccttgat atttccgcca gaggtcttta gccgcttcac caattccctg aacgccttct
    23101 ttcaccatat cgccgaagtt tttcagccct tcttttgctt gttcaccata ttgattcaag
    23161 ttatcttgcc aatttccaga ctctccatta gaatttcctg tgtttccaac ttgatctgca
    23221 gctaccactt gtcctcctgt ggcataggca tctgctacac taaatcctgt ttttgaagta
    23281 tacggaagga tgccttctgc cgctgatttg aagactttcc cgacaacatt tccacttgtc
    23341 ccttccaaat agtgcagctt gctggattct tcgtaaccca tccatgtcga aatcactacg
    23401 tctggcgtgt aaccgatcat ccattggtca tttgcttttg tggcatcgaa attagtttct
    23461 gtggttcctg ttttccctgc tatcgtataa cctgcaggat tcgcagcagc cgctgttcca
    23521 tttgaaaatg taccaagaag catgctggtc atttcatcgg ctgtttcctt tgatatgacc
    23581 tgttttggtt ttttgtttgt gttatcgacg atcaccgctc cagtcgaatc aacgattttc
    23641 gtgataagat gcggcgtata catctgacca tcattggcaa atgcactgta agcacccgcc
    23701 atgatcaatg gagatactcc tttttccagt ccaccaaggg ctaagccata atacttatca
    23761 gattttgcta aaggcaggcc gaattcttct gctttatcat atcctttttg taaaccgatc
    23821 tcatgcaaaa gccatacagt aggcaaattc aaactttgtg caacagcttg atacatcggt
    23881 acttctcctt ggtatgtccc gtcaaaatta tgcgcatcgt aataactttg cggttcgtct
    23941 ttcaagatac tggaaggctt gtatccagct tccagtgctg gtgtatatac gcttaatggt
    24001 ttgatcgttg atcctggtga acgacgcatc tgtgtagcaa agctgaatcc tctaaagaca
    24061 tgatctcctc ttcgtcctac tagtgcttgt acaccacctg tattcggatc taaagctact
    24121 gaagcagatt ggaccatggc accatctgaa gcatttgctg ggaataatgc actgttttga
    24181 taagtagctt ctaattgctt ttgataatcc tgatctaaag ctgtatagat tttatagcct
    24241 ttattcatga catcttcttc atcaagacca tactcgttaa ctgcttcatc gatcactgca
    24301 tcaaaataat aaggataacg ataaccagaa tctgaattat cgtatgtgtc attaagatac
    24361 gcgctgatat ctgtctggct ttctgcttgt tcttgttctt ttgtgatttt tccgttttct
    24421 tccattacac ttagtactgt gtttttccga tcattcgcat tttttgggtg atcgatcgga
    24481 ttataaatac caggaccttt cagcattcct gccaaagtag cagcctcaga cagcgtaaca
    24541 ttcgttgcat ctactccaaa atactttctg gaagcgtctt ggacacccca taccccgttg
    24601 ccaaaatagg cattgttaag atacatggtc aagatttctt ctttgctgta cttcttttcg
    24661 atctcgattg ccaaaaataa ttccttcgct tttcgatcaa acgtttggtc taatgtcaga
    24721 tacgcatttt tggcaagctg ctgagtgatc gtacttcctc cgccccctga tgttccgcca
    24781 gacaagacca ttcggactgc tgcacgggca atccctttga tatcaaaccc gtgatgctcg
    24841 taaaaattcc tatcttctgt cgagattact gcatcttgga tatagggaga aatcgcgttt
    24901 aattctacat atgttccttt ttgggcgtac agtgtgccag cttcttcgtt tttcttatca
    24961 taaatgactg tcgattggct taatcctgat tttaacgttt cgacatttgc ttgttttgct
    25021 aaaataaaga ggtagatact tgtcactaag acacagatca aaccaatcag caaaatcaat
    25081 ttgttcacat gatatttttt ccagatgcgc tttcgccatc gatggaattg tccgagatat
    25141 ggtctgatcc aacgccaaaa actgatcaaa gcagctttta tcttttctaa aatgctttga
    25201 aaatccattt gcttcttctc ctgtctattt gattcaatcg tcttatttta ccatagtgaa
    25261 agagtgtttt cttcctgctt aagttgtagt tcaatatgtt tttttacgtt agtgcaagta
    25321 gagtaacata agaatgaaca gaaaaatgta agttttttct taaatatgtt ttaaaaatgg
    25381 tacaattagt ctgcgattgc actgaaagga gtacacttgt gaaaatcacg atcactttac
    25441 ctatgaacca tcctgaaaca acaattcgcg acttgcttga aaaggaatgg ttagttccta
    25501 gaaaggttcg acactttttg cgtatacgta aaaacgtttg gatcaatcaa caacctgctt
    25561 tgttccatca tgaagtaaag ggcaatgacc agatcacatt gttattcgaa gaaacagatt
    25621 ataattatca agaagttcaa ttaggagatg cgaagcacat ccgtgtttta tatgaagatg
    25681 agcatttgat tatagtggat aagcctgcag gaatgaagac tcatccaaat gaaccgacag
    25741 aaaacagtac cttactcaat catttagctg cttatctcga cactaaacag caacgacctt
    25801 acgttgtcca ccgcttggac aaagaaacaa gcggtgccgt tttatttgca aaaaatccat
    25861 ttgtcttgcc gatacttgga agaatgttgg aacaaaaaat gatctatcgg agatatcaag
    25921 ccatcgtatg gggagaaatt gccaaagata tgaccatcac caataaaatt ggacgagatc
    25981 gccatgatcg gagaaaaagg gtcgtagatg ataaaaatgg tcagagcgcc ttgacacatg
    26041 tcactgtcaa caaagtactt tctgaacaaa cacaagtcta ttgtgttttg gaaaccggac
    26101 gtacacacca gatcagagtt catttagctc ataagggaca tccgatcgtt ggagaccctc
    26161 tctaccaaaa tagaacagct gaacgactga tgctccatgc attagaactg cacatgatcc
    26221 atccatttac tcaagaaaaa atagtcggta aagctttacc tggattatgg gaatagaaat
    26281 aaaaaagaac agagtcttat cagcagataa tgataagact ctgttctttt tgtgtaattt
    26341 gtctgcttac ttttgatgtt cgtgttctgc taacagcaaa ctaccgataa tcccactttc
    26401 attcggcagt ccccaacgaa cgatatattc acttacttga ggtgtttcca tataaccacc
    26461 catcaattca acaaattgct gacggatttt ttgaaggaga tgatcctgat tcattacccc
    26521 accgcctaaa ataatctttt caggtgctaa cgtcaacgta tagttgatca gtgcttgagc
    26581 aatataataa gcctgaatag cccaaacagg atgatcttgc ggcagatctt gccctttgat
    26641 ccctgtgcgt gcctcaatag acggtcctgc tgctaatcct tctaaacaat ctttatgata
    26701 aggacaagta ccttcataat ggtcatctgg atggcgtttc acccaaatat gtcccatttc
    26761 aggatgtgcg atccctgaaa aaatttcttc attgatcacg acacctgcac cgattcctgt
    26821 tcccacggta aggtaaatac aactattttt tccttgtgct gctccttgct tcaattcccc
    26881 atatgcagca gcattgacat ccgttgtcca agcagcagga atattgtagc gttccttgat
    26941 tgtgccaaga aaatcgaatt gtgcccaccc ttttttggga gttgccaaaa cgtatccgta
    27001 attatctttt gttttgtcga taccaatcgg gccgaatgag ccaatcccga ttcccttcaa
    27061 ttcataacga tcaaaaaatt caaatacctg atccaatgtt tcttctggtg ttgtggtagg
    27121 gatacttaat ttttctaaaa tttctccacc atctgaaacc gcacacacaa attttgttcc
    27181 gcctgcttct attccgccat acattcgttt tcctcctcga atccgattgt ccgaatctca
    27241 taaggttgga ttattgggac aaccgcttgc ttcgttggtt cttcaagcag attcaatatg
    27301 acagtttttc cattgtcttt agttatttcc aatttttcta aatgactaga catattgaaa
    27361 cctcttacta ctactttatc actaaacttg ctgcgtttca aagctgtaat tgcaaacgtt
    27421 tcacttttta tcgttaaata ttgttgtttt gaaatcaatg ttccttcatg atgtttgatt
    27481 tgttgagtgg aaaaaggaat ctgtgctgcg tatgcatgca aataagtaga gaatttttct
    27541 tctggtccat gtagttcaat gctgtaattg aaccgatgct cacctagaca ctgtgcttct
    27601 ggtgttggaa aatatcccca atcgcctagt tctcccacac tgcgaagtag cgtcactgcg
    27661 atttgtccgt cttctgttac ttcatattca ttcaaaccaa agtttccgac tgttacaccg
    27721 tactcttcat catgaatatt gacaaatgct tgctgatgtt gcggattcgt ggggttttcc
    27781 caacttttag acacatggtt tgggcgcttc accacttcaa aaatactatc tgcttcgtga
    27841 tgttccacat gtaactttgt aggaaacaag acacgtaagc gatgatcttt catctgattg
    27901 tcgagagtcg tttcaaagtc gattttttta ctatcttttc tgattgtcat gattgttttt
    27961 atttgaagga ttcgtttttc tttggatcgt tctgcttttc gataacgaaa ttccacaacc
    28021 atctgctgtt ctttttctaa acgctcgtct gcagaaacag ggatttccat ttcctgtaga
    28081 atacttattt ttgctatttc cggtgtatct actaatactt ttacttcaga gttttctttg
    28141 ttactggata ggattggctg atcacagaaa ggttgtttaa agatatactc atttcctata
    28201 tcgccgatat cttcaaaaat atgcagatta ttcaactttc gtttgttttt gcgatcttcc
    28261 attgtaatgg taccattttt ttcaatcgtc aaatgtagag ggccgttttc gatgattcgt
    28321 ccactctgat gaatcattgt ttccttttct tctgctttcg cttccccttc tacaagcgcg
    28381 aatgtttccc aagaaaatgc tggcatgttt tctaatggta aagtcacttt gatcattctt
    28441 gccatatatg gcacacggaa acggtctttc ggcaaatcat aaccaaattg gacagtttct
    28501 tcagataaaa tagctggtat agtggcccca ctttttgttt ctacatgata cttacgttta
    28561 ggcaaatttt ctaactcttg atatagttgt tcaggtattc cttcagcgaa tctttttctt
    28621 tctaattcga tggttatttc tgcttcacca gttttatcca ttcctgcagt atttacaatg
    28681 acaaatggaa agctgtcttt tgggaatccg gtcgtatcga ttgctgcggt cagctgttct
    28741 aaggaatctt gtgcaagata ctttccaact tcatctgctt tttcaaaacg aggtatcatt
    28801 tcccgatgga cagaatcgac tgaacaaccg caaatactgt cgtgaggatg attttgcatt
    28861 aatgtcttcc aagcataatc taattgatca tggggatagt ttccactaac ttcataagcc
    28921 atcgtcgcaa gtggttctgt aatattttct agctgtcgct ggaccttggt attccattgt
    28981 tttaagtaaa cccgagcaga tgctgtattg gctaaagtat accagccatc cgtttcttga
    29041 cttgtcaatt ctccttctac tgagcctaag tcttctggca catctttttg aactgcttcc
    29101 agatagtcag taaaattaga atgaataaac tcgtaatctg gaaacagttc atttgcgaga
    29161 tggattgctt ttgatatatc tttttgtacc ggttgatgat caacaccatt catcatcaat
    29221 aagtggttcg tagaggcata ttgttctgca tccgctaatt tttgcttcca aaaggcaaga
    29281 gctgcttctt tttctactgg aatctcattc ccgttgctat accaattggc aaatagcaga
    29341 ccaaagattg ctgtttgatc aggtcctttc caccacatct ctgagtattg ggaagaatag
    29401 ttttctgctt ccaaaacttg attatcaaag ccaattggtt ttacaccacg gccaaacgct
    29461 gctgctgaaa taccagcttg tttcattaat tgaggcgtct gtcccatatt tccaaatgta
    29521 tcgggaaaat agcctaacat gacaggggtt ccccatttac ggctttcttc catgccaatc
    29581 agcatatttc tcacgttcga ttccgagctg ataagaaaat catcttgcaa gatataaaat
    29641 ggtccaattc tcaattttcc ttcattgatt gcttgttgga ttgcctgtct tttttctggc
    29701 cttacttgaa gatagtcatc taaaataatc gtttgcccat ccaaatgaaa actgttgaag
    29761 gacggatcaa cttcaaatag ctctagtaga tcgtctatca attcaatcag tcgcatgtga
    29821 tgctgttcgt aagccatgta ccattcacga tcccagtggc tatgagaaat gatatatact
    29881 ttctttttca tttacgatac ctcctacttt tctactctaa tatcaaagta atccatgact
    29941 aattcgcaaa acatcatatt tgcccaagaa aaccattctc tcgtataatt ttcaggattg
    30001 ttcacatcga atccttcgtg cataaggtgt gttcctgcgt cagttgctac aagtaaattc
    30061 aatatccgct ctttttcaga tttgtctgac gttgtcattc cttccatagc taatgcaatc
    30121 ggccaaacgt atttttctgg cgtatgggag gatccaattc cttttgcgta ctttccttca
    30181 tagaaatatg gattttcttt actcaacaat gttcttcttg tttgcagata atcttcctct
    30241 tgttcagaac aatatcccaa gtaaggtgcg gcgatcagat tgggcacatt actgtcatcc
    30301 atgactgtgg cgtttcccat tccatctact tcatatgcat aaatcgtttg ccctttttga
    30361 ttaaccgttc gtccgtgatt ctgaatcccc tcttggatct ctttcttcaa tttctgtgtt
    30421 tgctgtacaa tttctttatc atcgagtatc gtttgaaaaa tttcttccac ataacctaac
    30481 acgaccaccg caaacatatt ggatggtacc agatatccat aacggcaagt atcatcgcta
    30541 ggacggaaac cagaccatgt cattcctgta aaagctgttt ttgcacctcg cccgtcattg
    30601 atcagcgtat cttcttttct tgtcgtgtct cgtacaaatg tataaggaga gtgttcatga
    30661 tcttgctctg ttttgaacac gtccaagatt tttttgatac cttcaataaa agattgattg
    30721 aattgatcag tttttcctgt atttttgtaa agaagatagg ccaactgtat tgggtagcat
    30781 aaagaatcaa tttcatattt tctttcccag atccaatcgt tcatttttgt atgatctgtt
    30841 tggtggccag caccgttagc ttcttcgttg aaggcattcg catatggatc gatattgata
    30901 taaaaaaatt gctttttgac taacccgctg atcatgtcag ctaattcctc atcttctttt
    30961 gcaatcacta aataaggacg aacttgtgca gtagagtctc ttaaccacat cgctggaatg
    31021 tctcccgtca ataaaaaggt ggttccgtca ttttgctttt tgactgttgt caacagagta
    31081 ttggcaaatg ctgctgtaaa attctttgcc cagtccgcat ggatatcccc acatttttgt
    31141 gtgatatctt ccataaattt ctgcaccgat tttggtatat ctttataagc cactaataaa
    31201 accttctttc tatttgatat atctttaaag attatacttc atacagccaa ttatgtctac
    31261 gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat ttgaatatga
    31321 tatatcatta agaggtgttt atatgaatca accattgtac cagacaatat ttaatgattt
    31381 gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa ctgaaaaaga
    31441 actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga ctgaattaga
    31501 gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat ccgcttctcc
    31561 ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc ttccatatgt
    31621 cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc aagataaaca
    31681 gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa aagagctaat
    31741 caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc ttgacttact
    31801 ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa tctatgagtt
    31861 accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg cctcttattt
    31921 gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc atccacaatc
    31981 cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt tgactttcca
    32041 cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata tcactaagta
    32101 tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca tgaatctctt
    32161 gagacagcat ggttatcata tccctgaaga tttttcaatt atcggatttg atgatatcca
    32221 agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg aacagttagg
    32281 taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac catccgatag
    32341 taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa atgaatatcg
    32401 gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat gggaactgcc
    32461 agccttatac tggcgttcct tttgggatga attattttgc accgcaaact acggatcaaa
    32521 acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt cttacccatc
    32581 aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc aatggaaaac
    32641 tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa agtatatttt
    32701 gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca ttgataccta
    32761 gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt ttacttttat
    32821 cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa ggaaaaatca
    32881 cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg agatttgaat
    32941 atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg atcccgttgt
    33001 attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc agctcggaac
    33061 aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac cttgctcaca
    33121 gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat gatcaacaac
    33181 aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa acattttatg
    33241 aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct ataaaacctg
    33301 gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat cctctttatt
    33361 ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac agctatagag
    33421 aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg cctgggacat
    33481 tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac ttgatgcctg
    33541 aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca aattatggac
    33601 gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac cacgaatcag
    33661 tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt gccaaggtac
    33721 tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat caaaatatct
    33781 ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc agagaaccat
    33841 tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa agcagctttg
    33901 ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag agatataaag
    33961 aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga tatggttttg
    34021 agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct atttcaaacc
    34081 agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa atggcacagc
    34141 cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt taccctggag
    34201 atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga ttttatcctg
    34261 tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag gctgttttgc
    34321 ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg cagcaacaat
    34381 ttatccataa aatcaaacgg atggacaaag aatatacgaa attatatttt acccatcaag
    34441 atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac cctaagaaat
    34501 atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag gttgtgaaaa
    34561 agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagatcctca tatttttcgt
    34621 ttggttggtc ttattaccgc agatgcaagc ttttgaacac cgtttattga gaggttgtga
    34681 aatcagcgtt ctgacctgag tattagagag acaaatggca aaatagttcc tcatattttg
    34741 ccattttttg agctaatacc gaggggcagc tgattgaaca ccgtttagtg gagaggtcgt
    34801 gaaaaagctg tttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt
    34861 ttgcataaat ttggcttaat accgagaaac tggcttttga actccgttta tcgagaggtc
    34921 atgaaaaagc tgccttgcct caagtaataa gaacagccca aaattagatt tctagacaat
    34981 tttttataag ttatttcatt acattggctt tttcttttat gattctccac tgttttctcg
    35041 gtactcttta ggggatctgc cgacgatttt cttgaataat ttagagaaat acgtggtgtt
    35101 gttgtagcca atctcacttg ctatctcatt gatgttttga ttactgtgca aaagcagttg
    35161 tttcgctttt tccatacgta aatggttcaa ataagctgaa aaactttttt tcgtttcttt
    35221 tttgaacagc tgtcctagat acatgacatt taaatgcagg ttttctgaca cttctttgag
    35281 tgtaagtggt tcttgataat gctggtgcag gattacgatt actttttcca cattgtcact
    35341 gtactttttc tgtctctgat gctttttgat gaatgacaga agtagctgct gaagttcttg
    35401 caccgttttt gcctgatgga ttttttcgat tccttgtaaa tattcctcat cttccagatc
    35461 aatcaattcc cgttgaatct ccataaacag taaaaaactg gaatatcgta tatcttccgg
    35521 catcatggct gctaactgaa actgttcaaa aaaatcagtg atcatttttt gagcgacttc
    35581 caattgttta ttttgcagca cgcgtctgaa acttgaaaaa tcgatggttt gatcttttgt
    35641 ttctgcttgt actgcgtaaa aaacatgctc gtctttatct ccataaaatt gatgcaactg
    35701 tagattgtct ttcgcttgtt ggaatgtttc agggattttg tccaaagaat ctgtctcttc
    35761 cccgatactg atcagccatt cctgatcagg aaagttttct tgaacaaaac aacagaagcc
    35821 atcaacttca tctgcctcca acaacaacgt gatcaataca cggtctccgt aatttcgttg
    35881 atagtaaaag atttgttgcc gttcttgcaa ccatctattg ataagagtat catgagttct
    35941 tgccagttga atcaatacta tcctatgctg cttctcactt atttgtccta ttatttcttc
    36001 ttcacttatt tcatccaatt cttcattcaa ccattgactg aataacagtt cttgatagat
    36061 ttcttgttga tttttttgtt cattttgctg atctaaccgt gttttgattt tcttgaggct
    36121 ttcaaccaat tcgaacttat tcacgggttt catcaaataa ttcacggcgc ctaattgcat
    36181 acctcctttc aaataatcga attcttggta tcctgataaa atcataaatt caaaattatg
    36241 acgttctttt tgagccgctt caataaattc cagtccattt atttctggca ttgtcacatc
    36301 cgttactact aaatcaatct gtcgctgttc aagtacagct agtccttctt tggcattctc
    36361 tgctgttgct actatttgaa agcctaaact ttgccaatca attatctttt ttaacccatt
    36421 caaaatcatg tattcatcat ctaccaataa cacttgatac acgctcatcc ctccttgaat
    36481 gtaatagaaa cggtcagtcc acctgtctca ttattctcaa gcgacatata ataactgctg
    36541 ccgaagctgg cacgtaaacg ttcattcacg ttttgcagac cgatagaacc gtgaagttcc
    36601 actttaggat gagacagttt ttcttcaatc tgttttagcc gtttttcagt catccctttt
    36661 ccattatctt tgatgatgat ccttacccgt ttgccttcct gcaagacttt gacacttagc
    36721 gcattatcga atcgagtaaa atcaataccg tgtttaaaat aattttcaac taatggctgg
    36781 atcacaaatt taggtacttc gatcttttcc aaatcaggat cgatcataaa atgataggct
    36841 actcgattag gataacgcat ttgatacaaa tagacatatt tttcgcaaaa gtccagttct
    36901 tcttttaaag tgatcgtttt ctcttgattc gtattgtttc gaagcaaagc ggaaaacgca
    36961 tacacaacat ctgctaattc ttcacttcct tcgctgattg catacattcg gatatattct
    37021 aatgtattat ataaaaagtg tggattgatc tgtgcttgaa gcgcccgcat atgtgcgtct
    37081 tgctgtttga tttctaattt gtagatgtct tctacaaact gattgatgct atccaacatt
    37141 tcgttgatac cgattgaaag ttccttcagt tcatattccg tgtttgttat gtcgatccgc
    37201 gtctctaaat tccctgctgc tgtttgatcc atggcctgca tgacgataga tacttgctgt
    37261 gaatatcgtt tgaatgtttt ataaagaaag ataagcagta tattgtctaa aataatgata
    37321 ccaatgatca gcggggcaag attttttccc gtttcgattt ttacactttt tttatctagt
    37381 acagctaata tacgataatc atcagacagt tcttggtgtt gaacaaaatt gtttttttcc
    37441 agatcgtcaa tcggcaattg tgaatttttc ttcagactct ctgtgatgat catttcttgt
    37501 ctttcaaacg tttctttagc tattttatta tcatggaatg tgtatagtcg atttgtcgtt
    37561 ccagaaatca tgtataaaga gagtccattg aatgacgtta gattttctaa aatcatatct
    37621 agttctttct tttgaaaacc aataaaaata cttcctgact cagctcctcc ttgtgtgata
    37681 ggcaacttga tataaaaggc gtctagtaat tttggtgtcc ccttttttat ttccccacct
    37741 ttgttttctc tagaagattc ataatactcc ggtagttgat tcaacacgat atatatcgca
    37801 cttagttctt cataatttgc gtagagatct ttgatttgat ttgggaaaga aactaattca
    37861 tcggtactgt tttgctgttc gtatacatac atcagatact gatctatggg ttttgttaag
    37921 taggtcgtca cattttcgat cttttcggaa ttccctacaa gctggttttt gatgatccta
    37981 ctcaaggttg ttttatcatt cagcattctg cttgtgcttt gaacggcatc acttgtcatg
    38041 ttttctgcct gtttttgatt ttgttcccaa gtatggatac taaaaataac ggtagcagtc
    38101 atcgttacac cgatcatgat aaccgcgtat ttccgcaaca gacgatttag taaggttcct
    38161 tttgaaaata gaatccgaaa cttattcata actttccaac tgtttatcta attcttcacg
    38221 ccaatgtgtt gtcatcgtac cattccaaac cgtcaaaaga ccaatgacac caaacaggat
    38281 caatcctttg aattgccaag tcacgcctaa aattataccg ctaccaataa ttatttttag
    38341 aaaagacgaa aagctggaaa aaaccgaaat aaaacttagc tttagcagat taggaacaga
    38401 gatcgtatat tcactatcta aaatcatact gtattgataa gtagcaaata ggcagagcaa
    38461 ggcaaaaagt aaaataaaat caatagctaa aaaaatgagc cctttgattt gaacagataa
    38521 atataaattg taactcaata aagcagtacc agataaaaat aaactaaata gcagattgcc
    38581 tcttaaaaaa tttcttttat atatattcca gcctcttttt attgtgattt ctttatactc
    38641 gaatccgtac aaataaaata gttcattgac tgttttccat gccgcaccta atccaaagat
    38701 gatcccgccg gcaaatgtat atatccagaa atacaaggtt agttttataa tgacccagat
    38761 ccggataaat aactcttcca aaaccttccc taacatatct tctcctccaa acattaagtt
    38821 tatactttct cattatacaa aaaaaggttg ggcaatcagc accttgacct gattattaga
    38881 gaaacaaacg gaaaaatagt tcctcatatt ttgtcgtttt gtgagcgaat accgagggtt
    38941 agctgattga acaccgttta ccaagagttt gtgaaatagc cgcttagttt cgagtagtaa
    39001 gaaaaatttt gtgaatatgg ctttccacat ttttacatat atttagctta ataccgagaa
    39061 actggctttt gaacaccgtt tattcggaac aagaggttgt gacacgtctt ttgtcacaac
    39121 ccctttgtct ttattcatta ttattcttgt cggaactcat catattgttt ttgcatttct
    39181 ttcaatacct tatcatagcc agccttgtcc aaagcgtctt tcaatttcgg taatgtttca
    39241 tctggatcaa cagtccctgt attcaatcca tctaaatatt gattcatgac attagaaata
    39301 ttacttaatt ctgttttgac actgtcagtg ttgaagctga atcctaaaat cggtgaagtt
    39361 tctgcatcag caatcgattg atcacgtttg gcaatcatgt catccgtaat agactcttgt
    39421 gtatatagaa tcttgttgtt tcctgtattc caagcagaca tatgtgtatt tggctgataa
    39481 ccatctagca atttgatttt atcatctgaa ccatcgactt tttcccatgc ttctccctcg
    39541 atcccccaaa caagcccatt caataattct ggatcactgt taagcagccc tagaagttct
    39601 actgattttt ctttattttt tgaagtattt gatacgacaa aatttgccat ttgtgcttga
    39661 gctgtagatt ttagtggaac agtaattgct tttgagacaa gttcttgttt tgctgcattt
    39721 gtcaaaattg tatcgccata atcatacggt ccttgtgttt ctccacgcat tagccaagta
    39781 tttccttcta gtggataatc cttattgctc gttgcagcgt cacttggaat gagtccttgt
    39841 ttgtaccagc tatgcattgt acgtaacagt ccgatatacg tttcattatc gtattggttg
    39901 atgattttcg aatcatctcc atccagatca atagcaaatg gtaacgtatt ccctaaagga
    39961 taatcaaagt tcccttggac tttgaagcct tgtccaatgg caaaagctgc cgtgtttggt
    40021 tctttttcat ggaattcctt caagactttt tctgcatcag cgtaagattg gattccatca
    40081 attgaaagat cgtatttatc tagtaaagct ttgttaaaag taagtacttg ttgagcaaag
    40141 acatttccat tgactgggaa agcatataat tttccattga ccagatttcc tttaatatat
    40201 gcttcatcta aatcattata agctttttca gcgtacttag gcgctaattc agtcaaatca
    40261 gcaaaggctc ctttttgggc attggaaaca tagttgttcg caaaggcgat atcataattt
    40321 tccccggaag aaatgatgac gttcattttc ttttcgtaat cgccccagcc aatgtaattg
    40381 atattgactt ttacaccgat ctctttttcg attcgtttgt ttgctacttc catcaattga
    40441 tcgaaattat ctggtttatc tccgatttga tacatctgca aagtgtccga attgctagct
    40501 tctgcctggt ctttagaacc agaacctccg catgctgcta atgttcccaa caacaacgct
    40561 gaaacacctg taaaggctag tttcttccat aacttcattt ttcttcctcc taattttatg
    40621 atttatattt ttgtttcttt attcttttac tccgccgatt gttagacctt tgacaaaatg
    40681 cttctggaaa aatggatagc tgatcgcaat aggcaacgtc gagatgacta cgatcgccat
    40741 tctggcagat tctcctggaa ctgaggctaa tccgcctgtc aattggcttc ctgctcctga
    40801 attttgtgtc agatattgaa tattactttg gattttcatc aaaagatact gtagtggaac
    40861 tagcttatca tcttggatgt atagtaaagc attgaaccag tcattccagt aacccaatgc
    40921 ggcaaataaa ctgattgtcg caatacctgg aacagctaac ggtaaaacga tctgaacaaa
    40981 tattcgcagt tcgctagctc catcgatccg tgccgattca ataatcgaat cagagaccga
    41041 acgtttaaag aatgtgcgca tcacaataat attgaaggga ctgacagcca ttggcaagat
    41101 caatgcccaa atcgtatctt ttaactgaag aagatttgtc ataacaagat aattggccac
    41161 catccctggt gagaacaaca tcgtaatcaa acaaaaaacg gtaaaaaaac gacggaatgg
    41221 aaagtttgac cgtgaaatcg cataagcata taaagaagta gccgtgctat tgatcagtgt
    41281 acctaaaaca gtaacaagta cagtgacgaa aagcgcttgg aaaattttat cctgcatctg
    41341 gtcgaacaag tacgtataac caaatatact gaacttttct ggccaaaaac tgtacccgtg
    41401 agtcgacaat atggactcat cggtaaaaga gatggcgata acaaatataa atggcaaaat
    41461 acaggaaata gcaaagagtg caatcattat attagagaaa aaattagccg tcttattgaa
    41521 agagcggact tggactctct ccactttctt ttttttcatt tttttcctcc ttagaataat
    41581 gcagaatcgc tatcaaaacg tcgggcgatc gtatttgtga tcatcaacaa gacaaagccg
    41641 acaactgatt ggtaaagtcc tgcagcagcc gtcataccaa aatctcctgt ggatgtcagg
    41701 ccattgtaga tatacgtatc caaaacttga gtcacatcat ataatgcacc ggaattccta
    41761 ggaatgttat aaaacaggcc aaaatcagct cggaaaatgt ttcctacggc taaaatcgtt
    41821 aaaatcgtca ttagtggaat aagttgtggt atcgtcacat gtttgatctg ctgccatttg
    41881 ctagctccat caaccatagc agcttcataa taagtcggat cgattcccat aacagatgca
    41941 aagtaaataa tgctgttata accaatccct ttccatgtac caatgaacac caagataaac
    42001 ggccaatatt tcggttcatt ataccaattg acagcttctc ctccatgtcc agtgatccac
    42061 tgattgaata ttcctttgtc aggacttaaa aaagcgtaaa caaagtaact gataatgacc
    42121 caagatagaa aataaggtag taaggacatc gtttgataaa ctttaacgag tcgtttattt
    42181 ctcaactcgc tcataatgat tgcaaatacg actgagatca gcagattcaa aaggataaat
    42241 gctacattat ataaaattgt atttttagtg atgagaaatg cttgatccga agaaaataaa
    42301 aatttgaaat tttcccaacc cacccaagga ctttccctca agctagccaa gaacccatct
    42361 gctgaaatat gaaaattttt gaatgctacg acattcgcca ttacaggtat gtaaaagaaa
    42421 aaaatcatcc aaagcatacc cggtacagcc atcaggacaa gtgctttata acgccataga
    42481 ttcataaaaa aatgattgaa cccctttcgt tttttcatct aaaaacccct ttcgattcat
    42541 tcgcaacgta attatagcgc tttcaataat gagaataaat aaacaaagta tagggaaaat
    42601 gaaacaaagt ataggagagg ttgtgaaatc agcgttttgg cctgagtatt agagaaaaaa
    42661 acgggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    42721 tgaacaccgt ttatcagagg ttgtgaattc agcgttttgg cctgagtatt agggagatcg
    42781 tgtccttact atctaactct gagtcacaag gaacaatggg gactgtaaca aaagtcttgt
    42841 cacagtccct tattccgaac aaacggtgtt ccagaagtag cttctacgga aataagccga
    42901 aatttcacaa aaaagccatc cgaaagtcag acggacaagt ctgcttttca gatggcgcta
    42961 caactactct gcttattctt ttagctatcc attcaattat ttttctctca cttgttctag
    43021 ctcttctttg gtcatgggtc gatattgccc tttattcaaa tctttaggca gccaaaattt
    43081 tcccatacgg attcttttta aatataagac ttcttttcct actgctttga ccattcgctt
    43141 aacttggtga aactttcctt catgcaaaat cagacggata attgaagttt ctgtctcttg
    43201 atctacttga tcaatttgga gttttgccgg caatgtctgc tcatcgccat caataatcag
    43261 tccttctgca aataaacgga tatcttcttc tgtcattata ccgctaacct cagctagata
    43321 ttctttctcc acatgttttt taggtgatag taactgatgg gcaaacaccc cgtcattcgt
    43381 taaaatcaag aggccctctg tgtctttatc taatcggccg actggaaaaa gatcttcccg
    43441 ataatcctgg tcttccaata aatcgattac ggtttgatca tgattatcct gagtagcaga
    43501 aatgactcct gcaggtttat gcaaaatgta gtaaaaatct ttttgatacg taatgatttc
    43561 tcctaaataa gtaaccgcat cttcgaattc atttacttgc gctttatcgc ttttcaccac
    43621 ttgtccattg acttcgatcg ttcctttttt gattaatcct ttgacttctt tgcggctgcc
    43681 aagtcccata tctgctaaaa acttatctaa acgcatgcgt ttcccccatt tcaaaagagc
    43741 atcaatctag ctcttctgat tcacttattt tatttttaat ttatgtcgta aacgcgctat
    43801 tcctggtcct agtagtcttt ctgccaagcg gattttcaaa gctaaataac catacactaa
    43861 agcccctata cctgctacta caagaatcaa caatagagac tggaatttgc tatcttcgtt
    43921 taaaaaagca ccaaaaatca tctttgtgat ccacgaagcc aaaagcataa tcaaggtaag
    43981 aataaagatc agcaatgtcc tgcgccagac aaaacttggt ttaaagcggg ctacttcatg
    44041 gattctcttt aagatcaagg tacaagacac agcaaatccg atcattgtcg ccaataaagg
    44101 gccgtatact tcaaataaac gaatggctgg gtattgaaga aggagtttca ctagaaaccc
    44161 aataaccaaa taattaattg ccgccttgtt ttcaaacatt ccttgcagca tgttggaaac
    44221 tagcatatat agtcctagga agagtcctac aaaacttgct tggatcagta cattacttcc
    44281 tagagaatcc ggtgtataaa ataatgtatt tagcggatag gcaagtaaca taacaccaaa
    44341 tgtcgccgga aacataaaaa atgaaaacaa ctgtaaattt ccgcttgtca attttgccag
    44401 acctactcga tctttcaacg taacagcttc tgtaatcaac ggtaacccag tagacgcgat
    44461 cgatgttgcc aatgcaataa ctaccatcgt caacttatct ggattggcac taaaaatcga
    44521 aaaaagatcc agtaactgtg cattggaata ctcggtggat gcagacatga tcttcataaa
    44581 agtgaactga tcgactaatt tgaagatcgt tacccctgaa cccacaataa taaacggaat
    44641 cgcttcttta acggtatcta acaacaattc cttcgttgca atatttattt tgttttcgct
    44701 gtagtgaata aacgccgatg tatacgcttg atgtttcttc aaaaaataca aaaggacaag
    44761 aatacttgct agcataccga taaatgccgc gaaagttgat tgcgtgaccg ccgtcacata
    44821 gtcaccatcc atgactttca tgatgatgaa tgtagccaat agcatataaa aaatacgtgc
    44881 cacctgttct acaatctgcg acagcgcata aggcatcatt tcttgattgc cttgcaaata
    44941 ccctcgtatc acactcatac aaggaaaaac caatacagcg atactcaaag aacgcatcgt
    45001 tggaatcagt tcttctccgc caccagaagc atgagctagc caaggagagg cgatgtacat
    45061 gaacaaagca aacaacaggc ctaacccgcc catcatctgt aatgcacgga taaataatcg
    45121 tcgtgaagtg ccatattcat tcaatgaatt atagcgtgca gtttgttttg caatcgcagc
    45181 aggtattcct gctgtagaga tcagtagaaa caatgcatat atattgtatc ccatattgaa
    45241 taatccattg gcagccttag cattttcgcc catccaagca taccagggaa tgatatagac
    45301 agcgcctagc aatcttgaaa tgatattact ggcggtcatc caagcagagc ctcgtgccat
    45361 tttttcttgc ggtgttaatt gatctaatga tggttttctt tgattatcca tcggtcagat
    45421 tccaactttc tacaaaacat tactatcatt ttaattggta aaacagaaac tggcaatcga
    45481 ttttatataa cttttataag aacaattgtt tttttgaatt cggtttcgtt tttttggtaa
    45541 agtaaagaaa tatacagcct tttatggcta acatttatag gagatatcaa tgacacaaga
    45601 aataaaaaaa ctacccgtta tatttttagg tcttttcttt atcacagttg gattgaattg
    45661 gttcttgctc cctcacgata tagcttccgc cggagtgggc gccatcggac atttagttga
    45721 aacggccttt tctatccaac gctcagttac tgtttggagc atcaatctta tgatgctgct
    45781 tctagccgct gtcctattag gaaaatcagt attttttaag actgtagcgg gaagtctgct
    45841 ttttcctgtt gttttagaga ttgttccaaa agtggaaatg atctcctcac atttcttttc
    45901 actgatttca ggaagtcttt tgttcagtct tggggtttac acattgtaca cagtcggggc
    45961 ctcaaatggc ggtataacga taccgccgat tattttccac aaattttttc ggcttccaat
    46021 gccgcaagga cttttactta ccaatagtct tatcgtcttt ttgaattatt atgtttttgg
    46081 attgttggag acactattcg ttctcctatc tattacagtc agttcattgt ttatgaaaat
    46141 actcatccgc ttgtcccctg tgtcaagaaa atttgaataa agctggattt tgtcatagcg
    46201 gaaacaaacg atatattaaa aagagtgtgc tataataaac caagaaattg aataaagcga
    46261 gggatcaatc aatgagcttc tctttagaag acatccgtca tttactatta aaagaaaatc
    46321 tattaaaaga atttgtttca acaagaggct ggcatcttga tgtaccagaa aaagctgaat
    46381 ttcttcagtt aagttatgat tcaagaaaag ctgatgcttc tactttgttt ttctgtaaag
    46441 gaatgaattt caaagaagaa tatttggatt cagcaatcga acagggtatt caatattatg
    46501 tatcagaaca accatacgaa aattcagcag taggaatcat cgtaacggat atccgtaaag
    46561 caatggcgct tcttgctatg gctttttacg actatccgca aaataaattg aaagtgatcg
    46621 gattcactgg aacaaaaggt aaaacaactg ctgcgtactt tacaaaagca atcttggatc
    46681 acacaacaaa taaaaaaaca gcattgcttt ctactatgaa tacaacgtta gatggcaaaa
    46741 catatttcaa atcccatttg acaacgcctg agtcattaga tttatatcgt atgatgtcag
    46801 aagcagttga aaatggtatg actcatctag tgatggaagt ttcttcacaa gcttacaaaa
    46861 cacaacgcgt atacggcttg actttagatg tcggaatctt cttgaatatc tcgcctgacc
    46921 atatcagtcc gatcgaacat ccgacatttg atgactattt ctattgcaag cgacaattga
    46981 tcttgaattc aaaaacagtt gttttaaatc atgaaagtga ctattttgat ttacttaggg
    47041 aaacagccga tctattccag attccaacga tcacttatgg tcgttcggaa gaaagcaatt
    47101 atcaagtgat ccgttcagat aaaggtactc acggttttac tttatcttct catgaagatc
    47161 gcttagctat ctgcgatact gcttatgata ttttgctggc tggaggattc aaccaagaaa
    47221 atgcagccag tgcgatcatt gccgcagcac taacaggtgc ctccagtcaa gatgcacaag
    47281 acggattaaa agaagctcgt gtaccaggcc gaatggatca attgatccaa tccaatggag
    47341 cgcatgtcta tgtggactat gcacataatt atctaagctt gaaaacattg ctgaaatttg
    47401 ccaaaaatga acatccagat ggccgtgtaa ttgttgtatt aggcagccca ggaaacaaag
    47461 caatctcccg tcgacatgat tttggaaaag tgctatcaga aacagctgac atcgcctttt
    47521 tgacggcaga tgacccagca tttgaagatc cgaaaaaaat cgcggaagaa atcaatgaag
    47581 caattactaa tccagatttg atcgttcatt atgaaatgga tcgtcctgaa gcgattcgac
    47641 gcgctttagc agaaagcgct ccgcaagatt cagtcgttat tgcgggaaaa ggcgtggatc
    47701 cctatcaaaa aatcaatggt gtagatgaac cttatgaagg ggattatgca atcgtgaagc
    47761 ggttgattga agaataatgg tatttatata ctagtggttt cccatttcta agaaaaaaag
    47821 ggactgtgat tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag
    47881 ctaattctgc tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa
    47941 atgggaacca gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc
    48001 aatcaccacc actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg
    48061 caagcatcgg tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca
    48121 tcggagctaa gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa
    48181 tgattggttc attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca
    48241 gctgatcaga tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc
    48301 caccaacggt tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg
    48361 cgttagctgc catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc
    48421 catggatccc tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc
    48481 atgtattcgt caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg
    48541 ttcctagtgc aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg
    48601 ggatcagagc agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga
    48661 cccaatttct cttgacacac atacaataca attcgaccgc aataatcgac ataataatag
    48721 ctgtaaagat tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta
    48781 ctacagtttc tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca
    48841 attcagggat acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa
    48901 catttaattt ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa
    48961 agtaaataga taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt
    49021 tgaaaaaagt tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta
    49081 aaaccaaaaa catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga
    49141 cagcccgaac gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca
    49201 aaaaatcaaa cattagttat tctcctttcc taaatataac gctttttcat attgtttaat
    49261 acggatatac tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc
    49321 actggcaaaa ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt
    49381 tgccataaaa ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa
    49441 attaaagaca ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt
    49501 tcgataaagc tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg
    49561 taaactactt cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa
    49621 aaagaaaaag gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa
    49681 actaagttgc tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct
    49741 tcctttctat ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt
    49801 catcgtcgtc atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt
    49861 cccgctggca tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc
    49921 atttgcttgt cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa
    49981 tgcttcatgt atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac
    50041 ttgtagtgtt tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt
    50101 gataaattta ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt
    50161 tagctggtca actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac
    50221 ataagaaggc gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc
    50281 agaaggaatc aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg
    50341 gactgccact gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt
    50401 tcgcatttct cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg
    50461 aagaatttct tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg
    50521 ttcggtgtaa atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga
    50581 atgatagggt atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa
    50641 aaaaatactg acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga
    50701 aataatcagg tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact
    50761 taccactgta atatttttac cgaattcact gactacgcga ttctttaata gttgggcact
    50821 gccgtaaccc gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc
    50881 caatgccgcc atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt
    50941 tttcaaagcc ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc
    51001 tttctttatc tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat
    51061 catcggtgac aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa
    51121 atgaaattcc tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc
    51181 attttgattt tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc
    51241 ttcttcggga aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac
    51301 ttgatactct actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc
    51361 ttctcggagc cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc
    51421 cgaaactttt aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa
    51481 actgatgtct gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg
    51541 gtttccaaaa aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca
    51601 tgtcccttga ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat
    51661 ttcttgcatg tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc
    51721 cataaaaaga atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc
    51781 ggactcataa aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa
    51841 aaacaagtca aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc
    51901 aatgatctta cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat
    51961 tgtccgatca gataataaca attcagaagc taaacgttga ctggtgacat actccttctt
    52021 gtgatcaatc aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta
    52081 ttccatcata tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg
    52141 acatacgctt ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg
    52201 aatttatcca aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc
    52261 ttaatcatat tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt
    52321 tgcttaaaga aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa
    52381 ataatcaatg cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc
    52441 ttgtaagcgt tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc
    52501 atatcttgaa taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg
    52561 ggatttagtg agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa
    52621 atttgcagaa gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa
    52681 cgagaaatat gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg
    52741 ttggaaagat gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa
    52801 cgaactccgt aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta
    52861 aaaagataca catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta
    52921 ttttttattc gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag
    52981 tatttcagtc accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat
    53041 gcggatcgtt actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa
    53101 agaattaaaa actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa
    53161 tataccgacc cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat
    53221 caatgagcca atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata
    53281 gctagataca atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata
    53341 ctctcctgca acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc
    53401 ctgcgaatac atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc
    53461 tcgatgtttt ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact
    53521 tgtttgtgta aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag
    53581 atatgaaaag ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa
    53641 tatgctgctt agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg
    53701 gacatacggt gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga
    53761 aatcttataa ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc
    53821 gtatggttta tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa
    53881 caatacgatg aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt
    53941 agaagctgct tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata
    54001 aaaatccacc tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag
    54061 gttgtaaata ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta
    54121 ttcatgtttt tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa
    54181 caactgagag aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag
    54241 acaaaaattt gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga
    54301 tcgttgctgc agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg
    54361 tagatggaaa taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact
    54421 ttgaataata taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta
    54481 aaaactgtcg aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt
    54541 caaatcatgt cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta
    54601 tttcgaaaag ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa
    54661 gctatttacc aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg
    54721 ttctcacgtt tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa
    54781 attaatcgag aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc
    54841 tgcactaatc ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag
    54901 ctcaccaaac atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc
    54961 acgcctttca aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa
    55021 gacccgactt gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca
    55081 actgatcgct gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat
    55141 taggcaaaaa acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta
    55201 ttaactagta ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt
    55261 tacttattta ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac
    55321 cccttctaaa atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat
    55381 gcttattcaa ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat
    55441 gaaaacaagt agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt
    55501 ttttggaaga tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc
    55561 agcaactttt ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt
    55621 gaaagttact ggtgtcgcat ccaattcata ccccgttggc gcttgagttt ctaccagctg
    55681 atatgctcct ggtgctaagc catctatcgc cagttttcca tctgctcctg tggttagacc
    55741 tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc
    55801 tccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc gatttgcttt
    55861 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgattg gtgtcgcatc
    55921 caattcatac cccgttggcg cttgggtttc taccagctga tacactcctg gtgctaagcc
    55981 gtcaatcgcc agttttccat ctgctcctgt ggttagacct gactgcagaa cggttccatt
    56041 ggcatcttgt aactcaaaaa cagctccttg caggacttcc ccgctttggt catcgatctt
    56101 tgtcaacacg acaccgcctg gtgtaagtcg gtttgctttc gtcaattcta ccaccgctgt
    56161 ctgacttctt tcgatctcaa actcgatcgg tgtcgcatct aattcatacc ccgttggcgc
    56221 ttgagtttct accagctgat atg
//
LOCUS       DAYYHB010000009        54197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000009 DAYYHB010000000
VERSION     DAYYHB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.009"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            512..1069
                     /locus_tag="ACHA6B_000479"
                     /pseudo
     CDS             512..1069
                     /locus_tag="ACHA6B_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="ACHA6B_000480"
     CDS             1127..3166
                     /locus_tag="ACHA6B_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8948082.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGSIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="ACHA6B_000481"
     CDS             complement(3275..4426)
                     /locus_tag="ACHA6B_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8948083.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="ACHA6B_000482"
     CDS             complement(4580..5110)
                     /locus_tag="ACHA6B_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8948084.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="ACHA6B_000483"
     CDS             complement(5209..5559)
                     /locus_tag="ACHA6B_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8948085.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACHA6B_000484"
     CDS             complement(5716..7245)
                     /gene="ahpF"
                     /locus_tag="ACHA6B_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8948086.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACHA6B_000485"
     CDS             complement(7255..7818)
                     /gene="ahpC"
                     /locus_tag="ACHA6B_000485"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8948087.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7962..8624)
                     /locus_tag="ACHA6B_000486"
     CDS             complement(7962..8624)
                     /locus_tag="ACHA6B_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8948088.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8808..10454
                     /locus_tag="ACHA6B_000487"
     CDS             8808..10454
                     /locus_tag="ACHA6B_000487"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8948089.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10445..11527
                     /gene="uxuA"
                     /locus_tag="ACHA6B_000488"
     CDS             10445..11527
                     /gene="uxuA"
                     /locus_tag="ACHA6B_000488"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8948090.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11635..13188)
                     /locus_tag="ACHA6B_000489"
     CDS             complement(11635..13188)
                     /locus_tag="ACHA6B_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948091.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13336..14625
                     /locus_tag="ACHA6B_000490"
     CDS             13336..14625
                     /locus_tag="ACHA6B_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8948092.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14751..15830)
                     /locus_tag="ACHA6B_000491"
     CDS             complement(14751..15830)
                     /locus_tag="ACHA6B_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8948093.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15939..16379)
                     /locus_tag="ACHA6B_000492"
     CDS             complement(15939..16379)
                     /locus_tag="ACHA6B_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8948094.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16436..16846)
                     /locus_tag="ACHA6B_000493"
     CDS             complement(16436..16846)
                     /locus_tag="ACHA6B_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8948095.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(17027..17461)
                     /locus_tag="ACHA6B_000494"
     CDS             complement(17027..17461)
                     /locus_tag="ACHA6B_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8948096.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17561..18409)
                     /locus_tag="ACHA6B_000495"
     CDS             complement(17561..18409)
                     /locus_tag="ACHA6B_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8948097.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18798..20564
                     /locus_tag="ACHA6B_000496"
     CDS             18798..20564
                     /locus_tag="ACHA6B_000496"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8948098.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20666..21925
                     /locus_tag="ACHA6B_000497"
     CDS             20666..21925
                     /locus_tag="ACHA6B_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8948099.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            22072..23373
                     /locus_tag="ACHA6B_000498"
     CDS             22072..23373
                     /locus_tag="ACHA6B_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948100.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23376..24227
                     /locus_tag="ACHA6B_000499"
     CDS             23376..24227
                     /locus_tag="ACHA6B_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8948101.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24240..25055
                     /locus_tag="ACHA6B_000500"
     CDS             24240..25055
                     /locus_tag="ACHA6B_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8948102.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            25206..26372
                     /locus_tag="ACHA6B_000501"
     CDS             25206..26372
                     /locus_tag="ACHA6B_000501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8948103.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26602..27249
                     /locus_tag="ACHA6B_000502"
     CDS             26602..27249
                     /locus_tag="ACHA6B_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8948104.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            27333..28277
                     /locus_tag="ACHA6B_000503"
     CDS             27333..28277
                     /locus_tag="ACHA6B_000503"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8948105.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28412..29863
                     /locus_tag="ACHA6B_000504"
     CDS             28412..29863
                     /locus_tag="ACHA6B_000504"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8948106.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29884..30720
                     /locus_tag="ACHA6B_000505"
     CDS             29884..30720
                     /locus_tag="ACHA6B_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8948107.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30720..31379
                     /locus_tag="ACHA6B_000506"
     CDS             30720..31379
                     /locus_tag="ACHA6B_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8948108.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31505..32194
                     /locus_tag="ACHA6B_000507"
     CDS             31505..32194
                     /locus_tag="ACHA6B_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948109.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            32200..33156
                     /locus_tag="ACHA6B_000508"
     CDS             32200..33156
                     /locus_tag="ACHA6B_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8948110.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            33200..33712
                     /locus_tag="ACHA6B_000509"
     CDS             33200..33712
                     /locus_tag="ACHA6B_000509"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8948111.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33968..34516)
                     /locus_tag="ACHA6B_000510"
     CDS             complement(33968..34516)
                     /locus_tag="ACHA6B_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8948112.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34605..35786)
                     /locus_tag="ACHA6B_000511"
     CDS             complement(34605..35786)
                     /locus_tag="ACHA6B_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8948113.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(36022..37530)
                     /locus_tag="ACHA6B_000512"
     CDS             complement(36022..37530)
                     /locus_tag="ACHA6B_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8948114.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37689..37880
                     /locus_tag="ACHA6B_000513"
     CDS             37689..37880
                     /locus_tag="ACHA6B_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948115.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            38083..39000
                     /locus_tag="ACHA6B_000514"
     CDS             38083..39000
                     /locus_tag="ACHA6B_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8948116.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38993..40066
                     /locus_tag="ACHA6B_000515"
     CDS             38993..40066
                     /locus_tag="ACHA6B_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948117.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            40214..40732
                     /locus_tag="ACHA6B_000516"
     CDS             40214..40732
                     /locus_tag="ACHA6B_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948118.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40713..41105
                     /locus_tag="ACHA6B_000517"
     CDS             40713..41105
                     /locus_tag="ACHA6B_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8948119.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            41181..42116
                     /locus_tag="ACHA6B_000518"
     CDS             41181..42116
                     /locus_tag="ACHA6B_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8948120.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            42249..43028
                     /locus_tag="ACHA6B_000519"
     CDS             42249..43028
                     /locus_tag="ACHA6B_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8948121.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            43021..43338
                     /locus_tag="ACHA6B_000520"
     CDS             43021..43338
                     /locus_tag="ACHA6B_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8948122.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43582..45192
                     /locus_tag="ACHA6B_000521"
     CDS             43582..45192
                     /locus_tag="ACHA6B_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8948123.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45349..46197
                     /locus_tag="ACHA6B_000522"
     CDS             45349..46197
                     /locus_tag="ACHA6B_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8948124.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46428..46907
                     /locus_tag="ACHA6B_000523"
     CDS             46428..46907
                     /locus_tag="ACHA6B_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8948125.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46940..49168
                     /locus_tag="ACHA6B_000524"
     CDS             46940..49168
                     /locus_tag="ACHA6B_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8948126.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            49297..50940
                     /locus_tag="ACHA6B_000525"
     CDS             49297..50940
                     /locus_tag="ACHA6B_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8948127.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(51013..51558)
                     /locus_tag="ACHA6B_000526"
     CDS             complement(51013..51558)
                     /locus_tag="ACHA6B_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8948128.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51581..52066)
                     /locus_tag="ACHA6B_000527"
     CDS             complement(51581..52066)
                     /locus_tag="ACHA6B_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8948129.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(52056..52868)
                     /locus_tag="ACHA6B_000528"
     CDS             complement(52056..52868)
                     /locus_tag="ACHA6B_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8948130.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(53007..54119)
                     /locus_tag="ACHA6B_000529"
                     /pseudo
     CDS             complement(53007..54119)
                     /locus_tag="ACHA6B_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT        18050 a         9848 c        10459 g        15840 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aatgattaca gcagcaatcg gtctcgacaa cggcagtatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     5701 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     5761 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     5821 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     5881 catttcgatc gttctatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     5941 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     6001 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     6061 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     6121 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     6181 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     6241 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     6301 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     6361 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     6421 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     6481 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     6541 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     6601 accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     6661 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     6721 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     6781 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     6841 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     6901 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     6961 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     7021 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     7081 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     7141 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     7201 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     7261 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     7321 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     7381 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     7441 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     7501 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     7561 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     7621 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     7681 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     7741 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     7801 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     7861 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     7921 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     7981 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     8041 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     8101 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     8161 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     8221 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     8281 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     8341 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     8401 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
     8461 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     8521 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     8581 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
     8641 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     8701 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgtataa
     8761 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     8821 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     8881 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     8941 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     9001 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     9061 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     9121 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     9181 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     9241 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     9301 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     9361 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     9421 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     9481 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     9541 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     9601 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     9661 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     9721 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     9781 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     9841 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     9901 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     9961 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
    10021 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    10081 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    10141 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    10201 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
    10261 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    10321 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    10381 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    10441 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    10501 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    10561 agaggtatgg cccagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    10621 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    10681 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    10741 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    10801 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    10861 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
    10921 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    10981 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    11041 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
    11101 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
    11161 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    11221 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    11281 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
    11341 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac gaatacatcc gtcctgatca
    11401 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    11461 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    11521 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    11581 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    11641 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    11701 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    11761 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    11821 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    11881 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    11941 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    12001 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    12061 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
    12121 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    12181 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    12241 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    12301 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    12361 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    12421 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    12481 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    12541 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    12601 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    12661 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    12721 gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt
    12781 cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga
    12841 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    12901 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    12961 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    13021 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    13081 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    13141 gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    13201 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    13261 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    13321 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    13381 ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca
    13441 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    13501 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    13561 tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct
    13621 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    13681 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    13741 gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag
    13801 caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    13861 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    13921 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa
    13981 cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc ggatgtgact
    14041 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14101 aatgaaactg aaaatccaga aaaaccagag gcagccccag aagaaaaacc agatgtaact
    14161 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    14221 aatgaaactg aaaatccaga aaaactagag gtagatccag aagaaaaacc agatgtgact
    14281 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
    14341 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
    14401 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
    14461 aaaagtagca aggatacaac ctttgctagt cctgaaacga aaaataaaca gctaccaaaa
    14521 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
    14581 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
    14641 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa ataccagatt
    14701 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
    14761 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
    14821 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
    14881 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
    14941 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
    15001 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
    15061 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
    15121 ccaaatacag ccaaggcgat tgtagtgagc aacgtgttta aaagagcgat aacaaattga
    15181 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
    15241 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
    15301 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
    15361 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
    15421 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
    15481 tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag catcgggata
    15541 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
    15601 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
    15661 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
    15721 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
    15781 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
    15841 aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
    15901 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
    15961 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
    16021 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
    16081 ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
    16141 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
    16201 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
    16261 agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg tctaatgatt
    16321 tccttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
    16381 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
    16441 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
    16501 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
    16561 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
    16621 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
    16681 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
    16741 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatga acttctccag
    16801 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
    16861 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
    16921 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
    16981 atatacaaaa aaatatcaga gacaaaaact aatctcatca taaacattac atatatttct
    17041 caaaaagttg ttttctttct tcaaaactta cttcacgatg gacctgatga agcatccata
    17101 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
    17161 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
    17221 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
    17281 gagacgtgtt ttcatccagc atatggaatc tcgtgccata caaggtaaaa atgacttcat
    17341 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
    17401 acagttctag tgctttcaaa ctatcattta atacaaaatc tatttccgtt cctttatgca
    17461 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagttaa
    17521 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
    17581 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
    17641 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
    17701 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
    17761 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
    17821 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
    17881 ataaaatagc ataaagaaac tcttctattc caaaaacaaa cagattaaga gaaacaacga
    17941 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
    18001 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    18061 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    18121 ctggaactat tgcaatgatt aaaggaaaaa acaagcttcc gaaaagtact ttaaaaaagg
    18181 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    18241 ctatagctcg gtcaaatcct aaaacagctt ccagtaatat tcctagaccg ctgacacctc
    18301 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    18361 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tatccacttc
    18421 cttaattcat taataatact cttatcttat tataacttac ttttcacctt aagttaactt
    18481 taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct agaatatact
    18541 tctacgttaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
    18601 agataaattc aggacactaa atagtacagg aagtagatta aaacttttaa aagttttaag
    18661 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
    18721 catttttgaa agcgttgtct tttgtttctc tatcacttat agtgatgacg tcatattaaa
    18781 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
    18841 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
    18901 aaaaagtata tgtgctttgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
    18961 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
    19021 tgacgagcga aacaaggcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
    19081 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
    19141 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
    19201 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
    19261 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gacttcttcg
    19321 ggggagattt gcaaggtgtg agggatcatt tggattactt gacggacctt ggcgtcaatg
    19381 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
    19441 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
    19501 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
    19561 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
    19621 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
    19681 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
    19741 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
    19801 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
    19861 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
    19921 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattc acagattcga
    19981 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
    20041 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
    20101 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
    20161 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
    20221 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
    20281 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
    20341 ttatttctga aggttctact cagtggctaa tagtatatga tcgtgaagat aagctctatt
    20401 tcacacggga actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
    20461 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
    20521 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
    20581 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
    20641 gttgaataca caggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
    20701 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
    20761 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
    20821 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
    20881 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
    20941 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
    21001 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
    21061 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
    21121 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
    21181 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
    21241 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
    21301 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
    21361 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
    21421 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
    21481 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
    21541 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
    21601 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
    21661 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
    21721 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
    21781 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
    21841 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
    21901 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
    21961 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
    22021 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
    22081 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
    22141 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
    22201 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
    22261 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
    22321 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
    22381 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
    22441 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
    22501 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
    22561 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
    22621 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
    22681 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
    22741 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
    22801 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
    22861 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
    22921 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
    22981 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
    23041 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
    23101 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
    23161 atgatctatc tgtttaacaa tggtggtccc ggaagcgtag gcggaggagc tggttctact
    23221 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
    23281 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
    23341 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
    23401 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
    23461 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
    23521 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
    23581 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
    23641 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
    23701 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
    23761 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
    23821 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
    23881 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
    23941 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
    24001 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
    24061 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
    24121 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
    24181 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
    24241 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
    24301 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
    24361 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
    24421 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
    24481 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
    24541 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
    24601 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
    24661 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
    24721 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
    24781 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
    24841 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
    24901 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
    24961 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
    25021 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
    25081 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
    25141 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
    25201 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
    25261 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
    25321 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt
    25381 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
    25441 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
    25501 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
    25561 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
    25621 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
    25681 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
    25741 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
    25801 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
    25861 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
    25921 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
    25981 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
    26041 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
    26101 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
    26161 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
    26221 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
    26281 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
    26341 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
    26401 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
    26461 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
    26521 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
    26581 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
    26641 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
    26701 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
    26761 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
    26821 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
    26881 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
    26941 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
    27001 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
    27061 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
    27121 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
    27181 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
    27241 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
    27301 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
    27361 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
    27421 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
    27481 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
    27541 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
    27601 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
    27661 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
    27721 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
    27781 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
    27841 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
    27901 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
    27961 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
    28021 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
    28081 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
    28141 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
    28201 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
    28261 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
    28321 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
    28381 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
    28441 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
    28501 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
    28561 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
    28621 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
    28681 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
    28741 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
    28801 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
    28861 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
    28921 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
    28981 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
    29041 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
    29101 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
    29161 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
    29221 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
    29281 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
    29341 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
    29401 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
    29461 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
    29521 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
    29581 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
    29641 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
    29701 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
    29761 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
    29821 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
    29881 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
    29941 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
    30001 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
    30061 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
    30121 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
    30181 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
    30241 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
    30301 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
    30361 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
    30421 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
    30481 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
    30541 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
    30601 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
    30661 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
    30721 tgtactttcc cctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
    30781 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt
    30841 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
    30901 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt
    30961 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
    31021 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
    31081 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
    31141 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
    31201 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
    31261 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
    31321 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa
    31381 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
    31441 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
    31501 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
    31561 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
    31621 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
    31681 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
    31741 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
    31801 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
    31861 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
    31921 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
    31981 ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
    32041 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
    32101 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
    32161 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
    32221 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
    32281 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
    32341 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
    32401 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
    32461 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
    32521 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
    32581 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
    32641 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
    32701 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
    32761 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
    32821 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
    32881 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
    32941 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
    33001 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
    33061 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
    33121 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
    33181 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
    33241 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
    33301 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
    33361 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc
    33421 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
    33481 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
    33541 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
    33601 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
    33661 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
    33721 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
    33781 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
    33841 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
    33901 tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc
    33961 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
    34021 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
    34081 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
    34141 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
    34201 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
    34261 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
    34321 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
    34381 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
    34441 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
    34501 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
    34561 aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca
    34621 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
    34681 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
    34741 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
    34801 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
    34861 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
    34921 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
    34981 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
    35041 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
    35101 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
    35161 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    35221 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    35281 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    35341 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    35401 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    35461 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    35521 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    35581 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    35641 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    35701 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    35761 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    35821 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    35881 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    35941 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    36001 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    36061 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    36121 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    36181 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    36241 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    36301 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    36361 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    36421 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    36481 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    36541 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    36601 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    36661 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    36721 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    36781 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    36841 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    36901 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    36961 cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    37021 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    37081 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    37141 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    37201 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    37261 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    37321 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    37381 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa
    37441 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    37501 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    37561 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    37621 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    37681 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    37741 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    37801 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    37861 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    37921 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    37981 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    38041 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    38101 aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa
    38161 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    38221 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    38281 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    38341 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    38401 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    38461 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    38521 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    38581 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    38641 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    38701 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    38761 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    38821 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    38881 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    38941 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    39001 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    39061 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    39121 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    39181 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    39241 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    39301 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    39361 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    39421 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    39481 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    39541 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    39601 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    39661 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    39721 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    39781 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    39841 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    39901 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    39961 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    40021 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    40081 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    40141 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    40201 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    40261 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    40321 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    40381 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    40441 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    40501 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    40561 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    40621 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    40681 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    40741 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    40801 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    40861 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
    40921 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    40981 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    41041 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    41101 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    41161 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    41221 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    41281 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    41341 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    41401 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    41461 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    41521 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    41581 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    41641 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    41701 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    41761 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    41821 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    41881 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    41941 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    42001 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    42061 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    42121 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    42181 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    42241 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    42301 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    42361 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    42421 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    42481 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    42541 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    42601 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    42661 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    42721 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    42781 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    42841 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    42901 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    42961 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    43021 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    43081 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    43141 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    43201 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    43261 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    43321 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    43381 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    43441 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    43501 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    43561 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    43621 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    43681 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    43741 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    43801 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    43861 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    43921 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    43981 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    44041 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    44101 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    44161 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    44221 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    44281 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    44341 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    44401 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    44461 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    44521 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    44581 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    44641 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    44701 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    44761 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    44821 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    44881 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    44941 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    45001 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    45061 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    45121 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    45181 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    45241 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    45301 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    45361 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    45421 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    45481 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    45541 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    45601 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    45661 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    45721 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    45781 cttgcacata tcgatcgaat ccgagataat acctacgctt atctgattgt agatgatttg
    45841 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    45901 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    45961 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    46021 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    46081 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    46141 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    46201 cttttattca aaaaacatac caaaaatatt ccaacatcat cttggacttt ctacatgtta
    46261 ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa gctctttttc
    46321 tttgcaatcc tcacttcaat cctctataat gtcgcatata gtttagtaaa tgagtaaact
    46381 actaatttac ttatttacta aaaataaaag gatgaggagt taaaataatg gaaataccaa
    46441 caaatttaaa acataaacca gtaatcgttg tagaagacta tgataaagtt gatggaagaa
    46501 atgcattaaa tacggatgca aaagggcttt cactgggcct tgctcagtgg aatgatcgtg
    46561 gaaaagtcga tatctcaggc aaggtatggc gacatactgg agaaaaatgg tcacgacagt
    46621 cggaagaatt accaatcaca cgtattttag atttagcgat cttagctgca caaggaagtt
    46681 tatacttcca agatgcttat cgttatgaaa aattttatga tccagaaaat ccagtcgtag
    46741 acatcattgg attacaaggg aaccgaatga cggtagaagt agataccaaa aatccaaaga
    46801 tcgatgaaga catcattttg tattacgata cactacaaaa agacggagaa ctgattgggg
    46861 aacgattccg tattttaaaa cgtcttttag atgagctagg atattaatag acagagatga
    46921 tcaaaagggg aagagaagca tggagaattt ctcactaaca gaaaaccgaa ccttttcacg
    46981 gctagaaaag caaatgatct ggaagaaacc gaaaagccat cggacaagtg cagaagaatg
    47041 gcggatcgcc tccgaaatca aagggaattg gcaagatcct gaaatgaaaa tcagtaacgt
    47101 acttttggaa ggagatgccg ggtcggggaa gacacaatta gccaaagcac tatctgctga
    47161 tctgcaactt ccctatacaa aagtgacctg ttttgcagat atggacaaat cagatgtttt
    47221 cggagcctta ttacctgtag tagattctaa taaggaacaa gaccaagaat tactagaagc
    47281 tatttaccaa acagatacac ttgaagctgt actgacactg atccagaacc attacggcat
    47341 tgatcctttt tctgctaaag aaaaattagc agagctagtt caaaggatcg aagctgatta
    47401 tccagatgca atccattaca gctattaccc ttctgaaatc gttcgagcga ttgaaaaagg
    47461 atatttatta gagattcaag agccgactgt catccgagat gcctctgttc ttgtggcact
    47521 taactcagcg ctcgaaccaa atgggatgtt gaatttaccg acaggaatca ttcgtcgcca
    47581 tccagattgc gtcattgtga tcactacaaa tcgaaattac caaggaaatc gcccattaaa
    47641 tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg gatcttcctg ccttagaagt
    47701 catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa caagaacttc tcttaaagat
    47761 ggcagagatt atccgtttgt tggatgaaac tgcaaaagct aacgccatca aaggtgtagc
    47821 aggaatgcgt tcttattttt attgggcaaa tacctataag caaggacaag atttactcca
    47881 gtcgatttat ccaaaagttt tgtataaatt atccactgat tcagatgaat tggcaattct
    47941 tgaacaagct ttgtctgaca gtggattgct actggaactt caggacatat tgagaaaaga
    48001 aaaatggggt catcctcaat ctgagcatac aaaaggaaga accattagcg ccgaagaagc
    48061 aaatgaacga aacatcactg aaaatgctga agaaattctg cgcttagatg aaaagaaatc
    48121 gttgtctgct gaagaaccaa atgagggaga agagaaacaa gaagaaacaa tagaaaaaaa
    48181 cgagattcct cctcaaatgc aaaaagaacg cagtagcgat atcgaaggaa aaaatcaaaa
    48241 agaacaacaa acaggagatg attctcaaga aatgtctgct tctgacatgg aagcacatga
    48301 taaggaaatc aagaagcaac tgaataaaga agcgcgacaa atcatgaagg aaacgattca
    48361 tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa ggaaaaacgc cggaagcacg
    48421 tgagctacac atgcaagtaa tgccaatagt agaatcactt agccggcaaa ttttagagtt
    48481 actggataat gaacaaacag agacgtacca aaaaggaaaa tacgaaggtc agcggttcaa
    48541 tgcaagcaaa gtagcatatg gtgatttgag aaattttgac aaaaagaacc cgccacatga
    48601 gcaaccttct ttagcggtag ctgtacggat cgatgaatct gggtcaatga tacgggatga
    48661 tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc gaattttcta agaaagtcga
    48721 tatcccattg ttgatttacg gagatacagc agaccgttcg tcaagagaaa agacttcttt
    48781 gttttcttat aaagaatttg aagacggatt tcaatggtta aatgaaaaat tagtcactat
    48841 gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt ttagcagcag aaaaattaaa
    48901 cagacaatcc gcaacaacca aactgttgct taatattagt gacggtcagc caaaagcgtt
    48961 gccggattat acaggcaaaa aagcaaaaga agatatccag tctgttttga aagaatatga
    49021 acgtcaagga atcctatttg tctccgcagc tattggccaa gacaaagaag aaatcaagtc
    49081 gatctacggt gaaagtcgtt ttgtagatat tactgacttg aatgaatttc ccaagcaaat
    49141 gattcaactg attgcccgct atctatgata aaataaagct caacgaacgc acttagcaga
    49201 ttcgttgagc ttttcgtttc tagttaccgg taacaaggat acttctaaaa tagaatcggt
    49261 tataatcaag agggaccgtt aaaggagtga aacgatatga tacaagaaca aaaatggtgg
    49321 aaagaagcag taggctatca aatctatcct gctagtttca aagatagcaa tggcgatggt
    49381 caaggagaca tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct tggtatcggc
    49441 tttatctgga tcaatccgat ctatcaatct ccatttgtcg ataatggtta tgacgtaagt
    49501 gactatcaag atatagaaga gagattcgga acgatggaag attttgattt attgctaaaa
    49561 gaagcacatg aattagggat caaagtgatt atggatcttg tcatcaacca tagttcggat
    49621 cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg cgattattac
    49681 atttgggtcg acggggtaga tggcaaagaa ccgaataatt ggacatcgat tttcggcggt
    49741 tctgcttggg agtattctca tgaaactgga cagtattatc tgcatgtttt tgcaaaagaa
    49801 caaccagact taaattggga aagcgagaaa ttgaaagaag aactattcaa tatgatccga
    49861 tggtggttgg ataaagggat agatggattt cgtttagatg cgatttctca tgtaaaaaaa
    49921 gatgaatatt cagtaaaagc cacagagaac cctttctcac cctttcaaaa tgtctcagga
    49981 atcgaagaac atttgaccga tttgaaacat gtttttgaag agtacgatat catgactgtc
    50041 ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa agacggttat
    50101 ttcgatatga tattcgaatt tgatcatatc catatctggc agcaagaaaa agaagggcaa
    50161 ttagatgtcc ttaaattgaa acttgcatta agcgcttggc aaacttcatt ggatggtatc
    50221 ggatggaacg ctttatatat ggaaaaccat gatgttcctc gtgccgtttc agtgtttgga
    50281 gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa tcgctatgat gtatttcttt
    50341 ttgcaaggta cgccatttat ttatcaagga caggaaatcg gaatgacgaa tatgcccttt
    50401 gaatcgatcg atcaagttga tgcagtagat tcgaaaagac tttataaaag gctgcttgcg
    50461 gaaggtaaga caagagaaga agcattggat atcattcgtg aaacaacgag agacaacagc
    50521 aggacaccca tgcaatggac ttcggaacaa tatgctggtt tttctacaca tgaaccttgg
    50581 ctgatcacta atccaaatac caaaacgatc aatgtagaac aacaagaata tgaaccagag
    50641 tcggtcctgc aattctataa gaacatgatc cgcattagac aaaccaataa aggtctgata
    50701 tacggttctt ataaagaata tcttcatgaa catccacaac tttatgtcta tgaaagatat
    50761 ttagaagatg aacaatatct tattatggta aacttaacag aatcacttgc tgactatgaa
    50821 cttccaaaag aagcagatca gtcctggaca ttactcttgt caaattcctc tagcggagaa
    50881 tttgaagcta aaggcatact agcaccttat gaagctcggt tatataaaac aaacaagtaa
    50941 aacttcctcg ttaaagttca ttagttggac tttgcgagga agtttttcag attgctctgt
    51001 caattactca tttcaataat tcgctctagg accaccgata tatttaggac gagagcgaag
    51061 ggctgtcacg tgagcttgtc caagggtttt caaacgaggt ctgacaggta cttggacatg
    51121 tttgatatgc ataccgattg atgtgtcacc gatatcgata ccggcttgtc caactacgtg
    51181 ttccacttcg acaggatcat tgaactggtc aaaagcagca acagaacaag cacctccagc
    51241 atgtagtgca ggaacaacag agacaatttc gaaatttttt gcttctgcca ctgttcgttc
    51301 tactactaat gctcggttca aatgttcaca accttggaca gctagcgcaa tttctttagg
    51361 atccaacaac tcttttaacg tacggatgat ccattgacca acttccgcac tggaattttt
    51421 tccaatatgt cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa gatcaccttt
    51481 tttcaagtgt gcctcagcca atatgtcatt aactatttca gtcaattggt cttttaattc
    51541 tttttctgac aacttcattt tctaaaacct tctttttcaa ttaatattgc ttccgtgctt
    51601 gaatacgttg aagacttgca gttataggaa aggcaagtcc cattccaatc agattttgga
    51661 agatgtttcc tggtatagaa gccactccag ccggccaatc atataaaatc caaccggcga
    51721 taccatagcc gatgatcata actaagcttc ctaataaaag ccctaatccc cacatacgag
    51781 tagaagattt gctgaaagaa ccagcaacat atccttgtaa cccgtggatc actaatgaaa
    51841 aaactgccca ctgcggatat cctgaaatca aatcgatcaa tagaccactg gaagcaccaa
    51901 caactaatcc acccgttgga ccataaagaa aagcagcggt atagatcccg gcctcgcata
    51961 atgtcacaaa tccattcgta gccggtacag ggatcaaaat tgttaatgaa agggcaactg
    52021 tcaacgcaat cagcatagca tagatcgtta attgcttagt tttcatttct cattcctcct
    52081 ttttgaatac cgccagacca gacacttccg gtatgatcgt tcatcagcac gccattagaa
    52141 atacaatggg caacaaattt tttactttct gctactgctt tggtaaaatc tgcccctaac
    52201 acaagattcg ctgttattgc agaagaaaag gcacaaccgg ccccgtttac tgttgtagtg
    52261 tcgatttttt tatgagaaaa tacctcataa tgatcaattg aatagagaag atccacagct
    52321 tcatctccag ccatccttgt gccgcctttg atcacaatgt tttttgttcc tgaagcagtg
    52381 agttctttag cgagatggat catttctgtt aacgaagagg gaagagcttg accagaaagt
    52441 aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag ttcttcgatg
    52501 agctgcgaaa tatacaattg attgatcaca gcttcagttt ctttgaatgc caaaacaggg
    52561 tctaaaatga ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac catgattgct
    52621 tcacttgaat tcaataaccc aattttgata ccatctaatg aagtcatttt atcaatcgaa
    52681 actaactgtc gtttaagcaa ttccaaagat atagattcca gcataaatcg ctcattaata
    52741 tctagtgccc ctatagaagt caaggcgctt agtccgaata gaccaaaatt ctcaaaagtt
    52801 tttaaatctg tttggattcc tccaccagca aatggatcag aaccgccaat tgtcaagatt
    52861 cttttcataa cattttcctc catgaataag agtataacga aattttcaaa aaagaaaaac
    52921 agccaattgg catattttta tctcatccaa tttaaaaaaa caaaaagaaa agaaagacca
    52981 aaatggatct ttctttcctt aaatagttag gacttttgtg attttaattc tttctcgatt
    53041 tgaagccgtt gcactttcat tgtagctgtc cgcgggattg catcaaaatc acggatgata
    53101 gggacgttca agcgaggaag ttcatggact tgctcccacc atcgattcca atccatttct
    53161 ttatcaggag ctaaagcaat aatcggttgt ggagaatgat ttttatccct tacaatcact
    53221 acttctgcca aaaaatctaa cgaatctaat aaaaagtctt caatagctaa tgtactattg
    53281 atcgtttcga tcaaatccac ttgccgatct ttcaaaaata ggtggccatg cttatccata
    53341 aaaccgtaat ctccgctatc ccaccaatcg ccataaacat tttcctggaa ccgtgcatct
    53401 tccttgtaat aagtcaaagc ccgtccttta gacagtaatt gtatatgacc atctgtgttt
    53461 tcaggtagga cattcccaac tgaatctgta atccgagcgc ttgtcatgtc ttccagtcca
    53521 acgcccatat ctcttgcatc acttgttttc aaactctcta gtgtatgagc cttaagaatc
    53581 attggtccgc attcactttg gccatatact tgaagaaata ccgcatcctg tgccatcgac
    53641 gttcgcaaaa atgtcttcat tgtctggttg tttatagcat caaaagtgga gtgatagtat
    53701 cggatacccg aaaaagcttc tggatgctcc ttagcagtaa aacgccactg aacaaagtta
    53761 ttcggatggg tctctagtgc aattggccgg tgagcttcca acatatgaac aacttttgaa
    53821 ctttgtgcgt ttgctaaagg catcatagga aatcccatgg acatcaaaga agagatacca
    53881 atattgaatc tggaatgcac tggagagatg tgaaaagcga ttaatttctt ttccgaaatt
    53941 tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca tagaatgagc agaatgacaa
    54001 ataagtttag ggataccagt tgtaccagaa gtatgggtca tataagaaat ttcatttttg
    54061 tctaaaaatg attgagaaac agacaaagca ggttgttcca ttaaataaag aataggaata
    54121 cccgtggtgc tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc
    54181 gttgtgctcg acttaat
//
LOCUS       DAYYHB010000010        51308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000010 DAYYHB010000000
VERSION     DAYYHB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.010"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHA6B_000530"
     CDS             330..1175
                     /locus_tag="ACHA6B_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8948131.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHA6B_000531"
     CDS             1456..2676
                     /locus_tag="ACHA6B_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8948132.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHA6B_000532"
     CDS             2673..5393
                     /locus_tag="ACHA6B_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8948133.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHA6B_000533"
     CDS             5377..6321
                     /locus_tag="ACHA6B_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8948134.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACHA6B_000534"
     CDS             complement(6390..7400)
                     /locus_tag="ACHA6B_000534"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8948135.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACHA6B_000535"
     CDS             complement(7580..7924)
                     /locus_tag="ACHA6B_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8948136.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACHA6B_000536"
     CDS             complement(7917..8351)
                     /locus_tag="ACHA6B_000536"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8948137.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACHA6B_000537"
     CDS             8489..9226
                     /locus_tag="ACHA6B_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948138.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACHA6B_000538"
     CDS             9219..10430
                     /locus_tag="ACHA6B_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948139.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACHA6B_000539"
     CDS             10634..11416
                     /locus_tag="ACHA6B_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8948140.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA6B_000540"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA6B_000540"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8948141.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA6B_000541"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA6B_000541"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8948142.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA6B_000542"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA6B_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8948143.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA6B_000543"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA6B_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8948144.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA6B_000544"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA6B_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8948145.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA6B_000545"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA6B_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8948146.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACHA6B_000546"
     CDS             16759..17106
                     /locus_tag="ACHA6B_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948147.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA6B_000547"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA6B_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8948148.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA6B_000548"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA6B_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8948149.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACHA6B_000549"
     CDS             17997..19004
                     /locus_tag="ACHA6B_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8948150.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACHA6B_000550"
     CDS             19029..20213
                     /locus_tag="ACHA6B_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8948151.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA6B_000551"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA6B_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8948152.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACHA6B_000552"
     CDS             complement(22338..22466)
                     /locus_tag="ACHA6B_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948153.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACHA6B_000553"
     CDS             complement(22811..22954)
                     /locus_tag="ACHA6B_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8948154.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="ACHA6B_000554"
     CDS             complement(23177..23656)
                     /locus_tag="ACHA6B_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8948155.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="ACHA6B_000555"
     CDS             complement(23729..24013)
                     /locus_tag="ACHA6B_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948156.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="ACHA6B_000556"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA6B_000556"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="ACHA6B_000557"
     CDS             complement(24357..25643)
                     /locus_tag="ACHA6B_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8948157.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="ACHA6B_000558"
     CDS             25781..26452
                     /locus_tag="ACHA6B_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8948158.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="ACHA6B_000559"
     CDS             26418..26906
                     /locus_tag="ACHA6B_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8948159.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="ACHA6B_000560"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="ACHA6B_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8948160.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="ACHA6B_000561"
     CDS             27867..28631
                     /locus_tag="ACHA6B_000561"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8948161.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="ACHA6B_000562"
     CDS             28864..31077
                     /locus_tag="ACHA6B_000562"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8948162.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /gene="dtd"
                     /locus_tag="ACHA6B_000563"
     CDS             31088..31534
                     /gene="dtd"
                     /locus_tag="ACHA6B_000563"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8948163.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="ACHA6B_000564"
     CDS             31705..32964
                     /locus_tag="ACHA6B_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8948164.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="ACHA6B_000565"
     CDS             complement(33053..34000)
                     /locus_tag="ACHA6B_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8948165.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /gene="hisS"
                     /locus_tag="ACHA6B_000566"
     CDS             34394..35686
                     /gene="hisS"
                     /locus_tag="ACHA6B_000566"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8948166.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="ACHA6B_000567"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="ACHA6B_000567"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8948167.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="ACHA6B_000568"
     CDS             37579..38454
                     /locus_tag="ACHA6B_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8948168.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="ACHA6B_000569"
     CDS             38720..40603
                     /locus_tag="ACHA6B_000569"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8948169.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="ACHA6B_000570"
     CDS             complement(40678..40962)
                     /locus_tag="ACHA6B_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8948170.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="ACHA6B_000571"
     CDS             complement(40952..41686)
                     /locus_tag="ACHA6B_000571"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8948171.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="ACHA6B_000572"
     CDS             41815..42438
                     /locus_tag="ACHA6B_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8948172.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="ACHA6B_000573"
     CDS             42630..43751
                     /locus_tag="ACHA6B_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8948173.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="ACHA6B_000574"
     CDS             43758..46886
                     /locus_tag="ACHA6B_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8948174.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="ACHA6B_000575"
     CDS             46905..50099
                     /locus_tag="ACHA6B_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8948175.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="ACHA6B_000576"
     CDS             complement(50245..50952)
                     /locus_tag="ACHA6B_000576"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8948176.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16792 a         8957 c        10646 g        14913 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccgc cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgc
//
LOCUS       DAYYHB010000011        50049 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000011 DAYYHB010000000
VERSION     DAYYHB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.011"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHA6B_000577"
     CDS             complement(59..187)
                     /locus_tag="ACHA6B_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8948177.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA6B_000578"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA6B_000578"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8948178.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHA6B_000579"
     CDS             complement(1674..2729)
                     /locus_tag="ACHA6B_000579"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8948179.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA6B_000580"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA6B_000580"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8948180.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA6B_000581"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA6B_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8948181.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA6B_000582"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA6B_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8948182.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA6B_000583"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA6B_000583"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8948183.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA6B_000584"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA6B_000584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8948184.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHA6B_000585"
     CDS             10491..12050
                     /locus_tag="ACHA6B_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8948185.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHA6B_000586"
     CDS             complement(12161..14092)
                     /locus_tag="ACHA6B_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8948186.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA6B_000587"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA6B_000587"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8948187.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHA6B_000588"
     CDS             complement(15021..15773)
                     /locus_tag="ACHA6B_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8948188.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACHA6B_000589"
     CDS             complement(15932..16141)
                     /locus_tag="ACHA6B_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948189.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACHA6B_000590"
     CDS             complement(16152..17480)
                     /locus_tag="ACHA6B_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948190.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACHA6B_000591"
     CDS             17616..17771
                     /locus_tag="ACHA6B_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8948191.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA6B_000592"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA6B_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8948192.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA6B_000593"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA6B_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8948193.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA6B_000594"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA6B_000594"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8948194.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACHA6B_000595"
     CDS             complement(21455..22387)
                     /locus_tag="ACHA6B_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948195.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACHA6B_000596"
     CDS             complement(22544..23203)
                     /locus_tag="ACHA6B_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8948196.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA6B_000597"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA6B_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8948197.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACHA6B_000598"
     CDS             complement(24244..24534)
                     /locus_tag="ACHA6B_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948198.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACHA6B_000599"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACHA6B_000599"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8948199.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACHA6B_000600"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACHA6B_000600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8948200.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACHA6B_000601"
     CDS             complement(28200..29369)
                     /locus_tag="ACHA6B_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8948201.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACHA6B_000602"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACHA6B_000602"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8948202.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACHA6B_000603"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACHA6B_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8948203.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACHA6B_000604"
     CDS             34320..34841
                     /locus_tag="ACHA6B_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8948204.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACHA6B_000605"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACHA6B_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACHA6B_000606"
     CDS             complement(36284..36940)
                     /locus_tag="ACHA6B_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948205.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACHA6B_000607"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACHA6B_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8948206.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACHA6B_000608"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACHA6B_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8948207.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACHA6B_000609"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACHA6B_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8948208.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACHA6B_000610"
     CDS             complement(40491..41222)
                     /locus_tag="ACHA6B_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8948209.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACHA6B_000611"
     CDS             complement(41226..42491)
                     /locus_tag="ACHA6B_000611"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8948210.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACHA6B_000612"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACHA6B_000612"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8948211.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACHA6B_000613"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACHA6B_000613"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8948212.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACHA6B_000614"
     CDS             47833..49335
                     /locus_tag="ACHA6B_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8948213.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..>50049
                     /locus_tag="ACHA6B_000615"
     CDS             49337..>50049
                     /locus_tag="ACHA6B_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8948214.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKD"
BASE COUNT        13811 a        10861 c         8813 g        16564 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtatcta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagattt
//
LOCUS       DAYYHB010000012        49923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000012 DAYYHB010000000
VERSION     DAYYHB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.012"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..514)
                     /locus_tag="ACHA6B_000616"
     CDS             complement(<1..514)
                     /locus_tag="ACHA6B_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8948215.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQV"
     gene            complement(591..1121)
                     /locus_tag="ACHA6B_000617"
     CDS             complement(591..1121)
                     /locus_tag="ACHA6B_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8948216.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1125..1985)
                     /gene="rlmB"
                     /locus_tag="ACHA6B_000618"
     CDS             complement(1125..1985)
                     /gene="rlmB"
                     /locus_tag="ACHA6B_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8948217.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1978..2373)
                     /locus_tag="ACHA6B_000619"
     CDS             complement(1978..2373)
                     /locus_tag="ACHA6B_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8948218.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2370..3782)
                     /gene="cysS"
                     /locus_tag="ACHA6B_000620"
     CDS             complement(2370..3782)
                     /gene="cysS"
                     /locus_tag="ACHA6B_000620"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8948219.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3779..4315)
                     /gene="epsC"
                     /locus_tag="ACHA6B_000621"
     CDS             complement(3779..4315)
                     /gene="epsC"
                     /locus_tag="ACHA6B_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8948220.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4401..4640)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4637..6097)
                     /gene="gltX"
                     /locus_tag="ACHA6B_000622"
     CDS             complement(4637..6097)
                     /gene="gltX"
                     /locus_tag="ACHA6B_000622"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8948221.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6267..7424)
                     /locus_tag="ACHA6B_000623"
     CDS             complement(6267..7424)
                     /locus_tag="ACHA6B_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8948222.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7458..8828)
                     /gene="radA"
                     /locus_tag="ACHA6B_000624"
     CDS             complement(7458..8828)
                     /gene="radA"
                     /locus_tag="ACHA6B_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8948223.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8891..9418)
                     /locus_tag="ACHA6B_000625"
     CDS             complement(8891..9418)
                     /locus_tag="ACHA6B_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8948224.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9648..10454
                     /gene="proB"
                     /locus_tag="ACHA6B_000626"
     CDS             9648..10454
                     /gene="proB"
                     /locus_tag="ACHA6B_000626"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8948225.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10451..11698
                     /locus_tag="ACHA6B_000627"
     CDS             10451..11698
                     /locus_tag="ACHA6B_000627"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8948226.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11774..12118
                     /locus_tag="ACHA6B_000628"
     CDS             11774..12118
                     /locus_tag="ACHA6B_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948227.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12235..12561)
                     /locus_tag="ACHA6B_000629"
     CDS             complement(12235..12561)
                     /locus_tag="ACHA6B_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948228.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13176..14378)
                     /locus_tag="ACHA6B_000630"
     CDS             complement(13176..14378)
                     /locus_tag="ACHA6B_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8948229.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14495..15010
                     /locus_tag="ACHA6B_000631"
     CDS             14495..15010
                     /locus_tag="ACHA6B_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8948230.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15209..15655)
                     /locus_tag="ACHA6B_000632"
     CDS             complement(15209..15655)
                     /locus_tag="ACHA6B_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8948231.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15702..17180)
                     /gene="msr(C)"
                     /locus_tag="ACHA6B_000633"
     CDS             complement(15702..17180)
                     /gene="msr(C)"
                     /locus_tag="ACHA6B_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8948232.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17534..18163)
                     /locus_tag="ACHA6B_000634"
     CDS             complement(17534..18163)
                     /locus_tag="ACHA6B_000634"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8948233.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18147..18611)
                     /locus_tag="ACHA6B_000635"
     CDS             complement(18147..18611)
                     /locus_tag="ACHA6B_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8948234.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19098..20366)
                     /locus_tag="ACHA6B_000636"
     CDS             complement(19098..20366)
                     /locus_tag="ACHA6B_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8948235.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20597..21334)
                     /locus_tag="ACHA6B_000637"
     CDS             complement(20597..21334)
                     /locus_tag="ACHA6B_000637"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8948236.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21401..21970)
                     /locus_tag="ACHA6B_000638"
     CDS             complement(21401..21970)
                     /locus_tag="ACHA6B_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8948237.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22098..22970
                     /locus_tag="ACHA6B_000639"
     CDS             22098..22970
                     /locus_tag="ACHA6B_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8948238.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23312..24184
                     /locus_tag="ACHA6B_000640"
     CDS             23312..24184
                     /locus_tag="ACHA6B_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8948239.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24181..26139
                     /gene="nagE"
                     /locus_tag="ACHA6B_000641"
     CDS             24181..26139
                     /gene="nagE"
                     /locus_tag="ACHA6B_000641"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8948240.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26471..27322)
                     /locus_tag="ACHA6B_000642"
     CDS             complement(26471..27322)
                     /locus_tag="ACHA6B_000642"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8948241.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27445..27912
                     /locus_tag="ACHA6B_000643"
     CDS             27445..27912
                     /locus_tag="ACHA6B_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8948242.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28175..28657)
                     /locus_tag="ACHA6B_000644"
     CDS             complement(28175..28657)
                     /locus_tag="ACHA6B_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8948243.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28757..29620)
                     /locus_tag="ACHA6B_000645"
     CDS             complement(28757..29620)
                     /locus_tag="ACHA6B_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8948244.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30218..31093
                     /locus_tag="ACHA6B_000646"
     CDS             30218..31093
                     /locus_tag="ACHA6B_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8948245.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31238..31390)
                     /locus_tag="ACHA6B_000647"
                     /pseudo
     CDS             complement(31238..31390)
                     /locus_tag="ACHA6B_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31658..32989)
                     /locus_tag="ACHA6B_000648"
     CDS             complement(31658..32989)
                     /locus_tag="ACHA6B_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8948246.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33138..33851)
                     /locus_tag="ACHA6B_000649"
     CDS             complement(33138..33851)
                     /locus_tag="ACHA6B_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8948247.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34026..35450
                     /locus_tag="ACHA6B_000650"
     CDS             34026..35450
                     /locus_tag="ACHA6B_000650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8948248.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            35749..36456
                     /locus_tag="ACHA6B_000651"
     CDS             35749..36456
                     /locus_tag="ACHA6B_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8948249.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36443..36766
                     /locus_tag="ACHA6B_000652"
     CDS             36443..36766
                     /locus_tag="ACHA6B_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8948250.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(36855..37892)
                     /locus_tag="ACHA6B_000653"
     CDS             complement(36855..37892)
                     /locus_tag="ACHA6B_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8948251.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38213..39196)
                     /locus_tag="ACHA6B_000654"
     CDS             complement(38213..39196)
                     /locus_tag="ACHA6B_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8948252.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(39611..40381)
                     /locus_tag="ACHA6B_000655"
     CDS             complement(39611..40381)
                     /locus_tag="ACHA6B_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948253.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40374..41309)
                     /locus_tag="ACHA6B_000656"
     CDS             complement(40374..41309)
                     /locus_tag="ACHA6B_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948254.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(41625..43274)
                     /locus_tag="ACHA6B_000657"
     CDS             complement(41625..43274)
                     /locus_tag="ACHA6B_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8948255.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43391..45994)
                     /locus_tag="ACHA6B_000658"
     CDS             complement(43391..45994)
                     /locus_tag="ACHA6B_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8948256.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46006..47055)
                     /locus_tag="ACHA6B_000659"
     CDS             complement(46006..47055)
                     /locus_tag="ACHA6B_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8948257.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47081..47395)
                     /locus_tag="ACHA6B_000660"
     CDS             complement(47081..47395)
                     /locus_tag="ACHA6B_000660"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8948258.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47411..47857)
                     /locus_tag="ACHA6B_000661"
     CDS             complement(47411..47857)
                     /locus_tag="ACHA6B_000661"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8948259.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(47858..49780)
                     /locus_tag="ACHA6B_000662"
     CDS             complement(47858..49780)
                     /locus_tag="ACHA6B_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8948260.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT        14652 a        10330 c         8677 g        16264 t
ORIGIN      
        1 ttacttgctt gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca
       61 tataataatg aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg
      121 cttcttttaa taaaagatgc tgaattgcta aaggttcttg caatgtgatc tctgctaaaa
      181 agtctggtcc ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac
      241 gcttcagcgc gttctgcttc ctcaacagag aatagatttg attgcttata acgcttttat
      301 aataacttcc aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag
      361 cgatccttgc ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt
      421 tcaaacgtag taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac
      481 cattacgtgc agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt
      541 atggaggaat cttatcatca atgattcatc gaactgatta gaacgatatg ttatttattt
      601 tttctcatta aatcgtccaa tttttgggac aattttttta actgctcttc attccatgga
      661 gaattacgtc ggaaatcaat aaaatgcata tctttatttt cttgggctat tgttttttcc
      721 gtttttttta tcgtcttata taattcataa gcagaagttc gtaatgctcc ttgggaaaag
      781 atcatccact gttctgctaa atcgcttgtc gccactgtca cttgtgtcaa acggtcattc
      841 agctgaccag cgattctctc gatataactg tcagccgttt cgtcttcttt cgtaaaaaca
      901 acatccaact ggtatttctt ataattttgt tggatgcccg gaactagttg ggcatcgaag
      961 acaacgatga cttccattcc ttcatatttc gcataatttg ataaacgatg taaaagagct
     1021 tctcttgcat ccgccatttt gtcctgcttt ttaagctgga caagttccgg ccacgaacca
     1081 atcatgttgt agccgtctac aatcaacagc tgttttttca tggtctactc cccctgacgt
     1141 tgggaaaaag cctgatacat aagcaaacca gcagcaacac tggcatttaa gctctggacg
     1201 tgcccggtca tcgggatcgt cagcaattca tccatttctt ttttcaatcc tggactcatt
     1261 cctttgcctt cattaccaat gactaaagca attgatcctt tggcattcca tttttggaag
     1321 tttgtgccgg tcatatctgt gccaaatacc caaaatcctg tttctttgag ctgttgcaca
     1381 gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc
     1441 tttgttacga taggtgtgat tccaactgct cggtgttttg gtatgatcac accatcgacg
     1501 ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt gtggatcttc gatgctatcc
     1561 aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc gttcaatcag ttggtcaagg
     1621 gataaatatt cataaggtgt aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc
     1681 aaagtttcta atttttgttt aggaacccat ttgactggca cagcttgttc acgtgcgatt
     1741 tctttcagtt cttctacttt ttctcctcgt gaatcttctt gaagaaagag tttattccct
     1801 cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa agacgaaatt atcttctaat
     1861 gcttcttcat tttgatcaga aactgactga tttcgtccag tttgcttttt atttgaatat
     1921 ctattttgtt ttttgtcttc aaatccacgt ttcgattgat gtttgtttgt tctttcttta
     1981 cccatttttt tcaccaactt tctgaataca ccagcggatc aattcttcca tccgctcggt
     2041 ttgttttgtc aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt
     2101 cgtaacatca gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata
     2161 aagttcttct tcttctgtca aaagatgttc ttccagcatt tgctgaatca aaaaggcttg
     2221 tgcattcgca gaaacataat tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc
     2281 ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc gcatctccaa cataagccaa
     2341 ggctaaaccg ttcaattgtg tgtaatctct catgcttctc ttctccatcg ggttccttgc
     2401 ggtgtatctt ctaataagat ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg
     2461 acaaaatcac gattgcttct tgcttgattt cgttcttcga tcaattgttc aatctcttca
     2521 tctaataact cttctgctgt aaataaaata ccgaagatcg aaagccattg cgagaataaa
     2581 gcttctcctt gtgccaagac agcggcagaa actttcggct ggtctagata agtgttcagc
     2641 cacttagcaa gctcatagac cactgtgatc ccatttgctg cattaaaatc atcgtccatt
     2701 tcttcgatga atcgctgttc taaattttct aacaaagcga ttttatccgc atcatctgcc
     2761 aaattctctg aggcagtttc ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt
     2821 tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt agcggatcgg acgacggtat
     2881 tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa cgtttttgat catttcatgt
     2941 acagtgatga aattccctag agatttactc attttttcat cgtcttcgcc aatcgttaca
     3001 tagccattgt gcatccagta attagcgaat tttttccctg ttttcgcttc actttgtgcg
     3061 atttcatttt catgatgagg aaattctaaa tcttgcccgc caccatggat atcgatcgtt
     3121 tctcctaaat gttttgttgc cataacggaa cattcaatat gccaaccggg acgtcctttg
     3181 ccccatggtg aatcccaaga aatctcgcca ggttttgctt gtttccataa agcgaaatct
     3241 aatgggtctt ctttttgctg ctgttcttcc cctgtacgct gacttgctcc gacttccagt
     3301 tcatcgatgg attggtcact taatttccca taattttcaa atttacgggt acggtaatac
     3361 acatcacctg aagattcata agcatagcct ttttcaacca acacttgaat aaatgctaaa
     3421 atatctggca tatgatccat gactcttgga tgcagtgtag caggttcgac attcaacgct
     3481 ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct
     3541 aattcgtttg ctgtacggat gattttatcg tctacatccg tgaaattcga tacgaaatcg
     3601 actttatagc ctcgatattc aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga
     3661 gcatttccga tatggatata attatacaca gtcggtccgc agacatacat accgacatgt
     3721 cccttttgta tagggatgaa ttcttctttt tttctggtca atgtattgta gatttttatc
     3781 attctgatct cctcactttt tctcctttga ttcgaacgac ttttgctggt actcctaccg
     3841 ctgtagcgtc gtctggaaca tctttcaaca caacggcacc tgcaccgatt ttggcgtttt
     3901 ttccgatcgt taccggtcct aatatctgcg cattcgcaga gagcatggct ccttgtttaa
     3961 cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc taatgttacg ccatggaata
     4021 aaatcacatc gtcctcgatt tctgcggttt cgccaatgac gattcccatt ccatggtcga
     4081 tgaacacgcc agttccaatc gttgctcctg gatggatctc aacaccagtt aggaaacgcc
     4141 agaattgagc attgatcttc gccaataaaa agagccggtg ctgatataaa aaatgagaga
     4201 aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac
     4261 gtgcagccgg atcgtttctc tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt
     4321 tccactcctt tcgtaagaat tttatgtagg aaatcatctt cttataaaga aaagaagcgc
     4381 cttttaaata agtttcctta tcaaaagacg ctttccgcgt ggttccactt ttcttcataa
     4441 tcaatgatta tctctgacag ctaacgcctg ttatacgttc ttggctactt tgtttcctat
     4501 aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa
     4561 ccattcattg ataccttgca ccacccggta tctctcttgt aatgatcagc tgcttacttt
     4621 tttctgttca tcgtgtttag ttcagtactt gatttaaatg tgctaatgat ttttcacgac
     4681 caagcaattc gatcgtttca ggcaattctg gaccatgcat ttgtcctgat acagctacac
     4741 ggattggcat gaaaagattt tttcctttta cgccagtttc tttttgaaca gctttgatgg
     4801 cagctttgac agttggagca tctactactt ccatttgttc tagctgtgct ttgaatgcgt
     4861 ggagaacagt aggaactgtt tcaccagcaa gtacttcttt tgctgcttca ttcaatacag
     4921 gatgttcatt gaaaaatagc gtagacagat caacgatttc tgcagcatag ctcatttgag
     4981 gttgatacag gctaacgatc ttcttcaccc attcgattgt cttttcatct gggttttctg
     5041 ctacacgtcc atcagcgatt agataaggca gacacatgtc agtaaatgca tctagatcca
     5101 ttgctttgat gtaatggttg tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg
     5161 atttacttag gcgttttgga tcgaacattt tgatcaattc ttggtgttcg aagatttcgt
     5221 cttcgcccac aggtgaccag cccaataatg caataaagtt gaacattgca tctggtagat
     5281 agcctaattc gcgatattgt tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt
     5341 ttttacctgt ttcactgttg atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc
     5401 caaatgcttc atagatcatc agttgttttg gtgtattggc aatatggtca tctccacgta
     5461 aaacgtgagt gattttcatc aaatgatcat cgaccgcaac agcaaaatta taagttggca
     5521 taccatcgcg tttttggatg acaaagtcgc caccgatatt atcggattcg aacgaaatag
     5581 cgcctttcac catatctgta aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa
     5641 cagattctag accttgtgct tctttttctg cttgttcttc aggtgtcaag ttcgcacatg
     5701 ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt
     5761 cttcttctgt gcaatagcat ttgtatgctc gattgctagc aagcagctga tcaatcagcg
     5821 gttgatagat ttctttacgt tcagattgac ggtaagggcc gtattctcca ggattttcag
     5881 gagattcatc ccaattcatt cccaaccaag caaggttttc caactggctt ttttcgccat
     5941 cttcgatatt acgtttttgg tcagtatcct caatacgaat aataaagtct ccatcattgt
     6001 ggcgggcaaa caaataattg aataatgcag ttcttgcatt tccaatatgc aggtgcccag
     6061 ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt cttcctcttc tttcaaacta
     6121 gactttgact aacagcaaaa attccgtcag ttacggcttc tcaaatatca tttatttaaa
     6181 gccactcagc acaaatttta ccttttctaa ggtaaatagt aaagccaact ggttgaattt
     6241 ccttaccagt tggctgaatt tcttttttat tttgattgtt tagtcttttt ctctgtctct
     6301 tttttattgt cgattcctcg tcctgcatgt gcaggtttag caaagatcat gcgtccagca
     6361 gctgtttgaa gggcacttgt tacaacgact tcgatacgtt cattcatgta atgctgtcca
     6421 tcttctacga caaccatcgt gccgtcatca aggtaggcta ctccttgttg gcgttctgtc
     6481 ccggctttta ctactagaac attcatcgtt tcaccaggga tgacgactgg ttttacggca
     6541 tttgccaacg cattgatatt aagaacaggt acattttgga attcagagac tttattcaag
     6601 ttatagtcat tggttacaat gacaccatct aaaagcttag ccaatttgat caatttgctg
     6661 tctacttcac tgatatcttc aaagtctcca tcgtacattt cgacagatat accctcttct
     6721 ttttgcaagg catttaggat atctaatccg cgacgaccac gcacacgttt caaactatcc
     6781 cctgagtccg cgatgtattg tagttcgtaa agaacaaaat tagggatcat caaggttcct
     6841 tctaagaatc cagtctttgt aatatcataa atccgaccgt caatgatcac gcttgtatcc
     6901 aagattttat atttatggaa attttctcct gattttcgtt ctaacagttg actctcaact
     6961 tgactagctg tttcttgctc gatttttttg cttcgtgaac caaaaacttt cttccattct
     7021 tcgattcgtg tcgttcccat tctcaatccc agatagccta agatgatcat cagtaaaatc
     7081 ggcaatacac tattgacgaa tggaatttcc atattgtaaa gaggggtcga gatcacaaca
     7141 cctaaagtca atccgatgat cgcaccgatc gcaccgaaga gaagataagt caagctgacc
     7201 tcacttagtt tttgttctac ttgtttgata atagctgaga tatgtttggc caacagcaaa
     7261 gataataaaa agaaaataag tgcgcctaac aggctgttgg taaatgtatt attcagccat
     7321 ccgtttgact gttgaccgat tgcctgccaa gctgagggta aaaatgaaat acccaagctt
     7381 gcgccgacaa tgatcatcag caaagtaatg acacgttttt gcatcgtttt tcctccgttt
     7441 cgacaatcat ttattagtta gcggaacaca cgcttcaacg tttcagccaa agttgctact
     7501 ccaactatct cgattccttt tggcgcttcc cagttaccta agttgttttt aggtaggtat
     7561 acttttgtga agccgagttt ttggacttct ttgactcgtt gttcaatatg actgacacgt
     7621 ctgatttctc cagtcagtcc aatttcaccg atgaaacatt ccgttggctg tgttcctttt
     7681 tctttatagc ttgaagcaat actgacagcg atagctaaat caatcgctgg ttcatttaat
     7741 tttaccccac ctgcagcttt taagtaagca tcttgattct gaagaagcaa cccagctctt
     7801 ttttccagca ctgccatgat cagagatacc cgattgaaat caagacctgt tgttgttcgt
     7861 ttggcatttc cgaacatagt aggtgtgact aatgcttgga tctctaccaa aattggacgt
     7921 gtcccttcca ttgccacaac gatagcagag ccagttgcac cgtctaggcg ttcttctaag
     7981 aaaatctgtg aaggattagc gacttcttcc aatccatgct catgcatttc aaaaatccca
     8041 atctcattcg ttgagccaaa tcggtttttt actgctcgca aaatccgaaa actatgatgt
     8101 tgttcacctt caaaatataa caccgtatcc accatatgtt ctaacattct tgggccggcg
     8161 atcgagcctt ctttggtaac atgaccgacg ataaaaatgg ctatcccatt tgttttagcg
     8221 gttttgagta gttctgcagt ggtttctctt acttggctga cacttcctgc cacacttgtg
     8281 atgtctggct gcgtcatcgt ttggatggaa tcaatgataa cataatcagg ggagatatgt
     8341 tcgatggcac gactgatttc gttcatatct gtttcagcgt atagataaaa ttctgtgttg
     8401 atacttccga ggcgttccgc gcgtaatttt atctgctctg cgctttcttc gccggataca
     8461 tataagactt ttccgcctat ggctgctaat tgctgagaaa cttgtaacag caaagtggat
     8521 ttcccgatac ctggatcgcc accaatcaag accatagacc ctggcaccac acctccgcct
     8581 aacacacggt tcagttcttc taattttgtt ttgatccgag gttctttttt agggacgacg
     8641 tctgcgattt ttgttggttt tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc
     8701 gttgtatctt gttcgatttc ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt
     8761 cccaagaact taggcgacac atacccgcaa ttttggcaaa caaattgtac ttttgatttt
     8821 tttgccattg taccgcctcc ttttatccta atcatgagac attaaatcaa gcttaagtat
     8881 ctttaacgtt ttatttactg gaagaaccaa atccgccttg acgttgagca gtcgtttgat
     8941 cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc gatacgctcg ccttttttga
     9001 tcactacatc cttgaaacca aagttggtga attggaacat gatatggcct tcatttgcct
     9061 cattattgta ataatcactg tcaatcacac ctactccgtt tgtcaacact aaaaaccgct
     9121 tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc atcttctccc atgtaagctt
     9181 tgattccagt aggcacgagg gttggtttca attgtttttc attttctgat acatcttttt
     9241 tcaatccatc tgcgactgtt tccaacccct ttttccagcc tgctttccag atacttggaa
     9301 tcacgacctc ttctgctgct tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg
     9361 gtagattgat attttgatct ttatatttag aaataacttc aaatcctctt tttctcaccg
     9421 gttttcctcc ctaaataaac tactcctatt ctaacatatc cgaggctgta aaagactttc
     9481 tttacaaatt cttcaagaaa ttctgctatc ttaaagtaaa ccattaattc gttctttttc
     9541 caaacgactg caagttgtac aaaaaaacta ttttttacat gactttactc gtcaaaacaa
     9601 gtagaataga acgaaattaa tgagtggata tttgattgag gtgtcacatg cgtaaagaaa
     9661 ttaaaaatgc aaaacgaatt attgtcaaag taggaacaag tacattgatt tatcccaatg
     9721 gaaatatcaa tcttgcggca atcgatcagt tagcttttgt cctttctgat cttagaaatc
     9781 ggggaaagga agtgatctta gtctcatcag gagctgtagg cgttggtatg cataaattac
     9841 atttgtcaga acgtccgact ttgattcctg aacagcaagc tgcggcggct gttggtcaag
     9901 ccgaactgat gaatatctat aatcaaagat tcagtgtata taatcaacag acggcacaga
     9961 tcctcgtgac aagagacgtg gtagattatc ctgaaagtcg taaaaatgtg aggaatacat
    10021 tggaacagct tttgattatg gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag
    10081 tggatgaatt agatcatctg acaaaatttg gcgataatga tcagctgtca gccatcatgg
    10141 caaaacttgt cgcggcagac cttttgatcg tcctttctga tatcgacggg ttttattcag
    10201 ataatccgac tacaaatccc gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa
    10261 agattctggc acaagcaggt ggtgctggta gcacatttgg aacaggcggt atgcagagta
    10321 aattaaaagc cgcttctagg atatttgaag caaacagcgc aatgatttta gcaaatggaa
    10381 aaaatccggc aattattttt gatattcttg caggaaaaga agtaggtact ttatttaagg
    10441 aggacttgaa atgactgact tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca
    10501 actagcactt atgcctacaa accgcaaaaa tgagctactt gttcaaatgg cacaaaccat
    10561 caaaaagaat caacaagcga tcattgaagc aaatcataaa gacttagaaa aagcagtcga
    10621 aaacaatatc agtgaaacga tgcaagaccg cttgcagctg acacctgagc gaatcaatgc
    10681 catggctaca gagatcgaaa aaattgcttc gttagacgat cctattggaa aagtagatga
    10741 gatgtggacg aacaccgacg gattgagaat cggcaaaaag cgcgtgccac taggcgtgat
    10801 cggcatcatt tacgaatctc gtccaaatgt cacgacagat gcagctagtc tagcattcaa
    10861 atcagggaat gccgttatcc ttcgcggtgg caaagaagca ttcttctcga atcaattatt
    10921 agtccaatta ttgcagcaag tactggtcag tgctggcgaa tctccttatg ccatccagtt
    10981 tgtcgatgat acttctcatg aaactgcagg acagttgatg cgattgacag aatacttaga
    11041 tgtgctgatt cctcgaggcg gagcaaaatt aatccaacga gtcaaagaac aggcaacggt
    11101 acctatcatt gaaacaggta caggaaataa tcacgtatat atcgataaag aagcacagtt
    11161 atctatggca gtgaacatca tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc
    11221 ggcagaaaca ttgctgatcc attctgagat cgcacctttt ttcttgccag ctattgaaaa
    11281 agaactggta gaacacgggg tctctttacg tgcggatgct cgtgcgttag aatatctaga
    11341 gacggctgct ttagcagaag acacagattg ggatactgaa tatctagatt atatccttgc
    11401 tgtcaaagtg gtcgattctt tggatgaagc aattgcacat atcaatcggc ataatactaa
    11461 acattcagaa acgatcgtga cagatagtta tgcggctagt caacgtttct taaatgaagt
    11521 ggacgcggcg gctgtctatg tcaatgcctc cactcgtttt acagacggat cggtatttgg
    11581 ttttggtgca gagattggta tctctactca aaagttgcat gcccgtggtc caatggggtt
    11641 aaatgaatta acttccacaa aatatatcat ttatggcgat ggacaaatcc gcgaataatg
    11701 tttcgtgtga aacattattc ccgccttttg ccttttttct gtaaaggttc tatccaacta
    11761 gaaaggtgtc aatatgtttg aaattttaat gatttttttt atttacctta tttctctgaa
    11821 tatcgctgct tttctgggag tcggtatttt atcccttttc tttcaattca aaaaacgaag
    11881 catagggagt caacgggaaa aatggtcgca gtattttgac aaaatcgggc aaaaagggct
    11941 ggttacacgc ttgcacatta gttacatggt tgcattatgc ttattagcga caatcaatta
    12001 ctattctttt ttcgatcatt caatcgctta tacgattacg ctactgattg ctgggatttt
    12061 tcacttatct tataaatatc agctgaataa aaaccacttg aatcggactt tccgctaaaa
    12121 aatatccgta cgataagtag agtgccttga agatcggcaa aaaactgccg gactttttag
    12181 aggctgtgac aaagattttg tcacagcctc tttttccata taaacggcgt tcgcctacca
    12241 gctgcctggt aaatctgttt cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc
    12301 atcataataa cgaagcagct catccatgga caagttcatc gttttttctt tttcttcttc
    12361 cgctgattca gccagtagct gttcccgata cacctgatac ataccagcta ttttttgctg
    12421 catcgctttt ctagtttcac cttccgcatt gattattggc aattctttga tatagccacg
    12481 ataaatcaac cgatcatctg aaaattcaat gggtaattca acgagtgtca acccgacttc
    12541 ctgaaaatat tcttccagca tctattatcc ccccattctt tttcttaaat gaattttagc
    12601 ttacctactg aaaattttcc agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa
    12661 tcgtgtcaca tccccttttt ccgaataaat ggtgttcttc tgccaactcc tcaatcataa
    12721 gtcacaatat tcaacaaaca tgagaaactg ttttttgaaa ttgttccttg tgattcagag
    12781 ttaaacgaca gtctcacaac ctcttggtaa acggcgttca atcagctgac cctcggtatt
    12841 agctcaaaaa acggtaaaat atgagaagct atttttccgt tttgtttctc taatacttag
    12901 gataaaacgc tgattttaca acctctgtta aacggtgttc aatcagctgt ccttcggtat
    12961 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg tttgtttctc taatactcag
    13021 gacaaaacgc tgattttaca acctcctgat aaacggtgtt caaaagcttg catctgcggt
    13081 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
    13141 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
    13201 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
    13261 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
    13321 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
    13381 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
    13441 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
    13501 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
    13561 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
    13621 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
    13681 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
    13741 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
    13801 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
    13861 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
    13921 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
    13981 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
    14041 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
    14101 cctgtcatca atgttttttt caaaccgatc cgactagata agaaacctgt aaatagcatc
    14161 accacaccag tgactaacat gtaactagta aataacaggg tcgtttcagc tggcgtagcg
    14221 tttaactgtg cagcaatcgt ttttaagatt gggtctacta gacccactcc cataaaagcg
    14281 atcatacagg tagctgctac aactagcgct gaacttttcc cctttgtttc ttcatttgtt
    14341 tgttcttgta tatctatttg attctccaat atttccatcc ctttctctct attagcatca
    14401 tacatgattt attttacatg tcaatatgca catgtaaaac gtggaaactt tactgtgata
    14461 taataaagaa aataagacga gaggtaggat ttttatgaat actttagcct atatattatt
    14521 atgcgtgctt ttatccaaac cacgttctgg gtatgaattg aagcagctga tcacaatttt
    14581 ttgggaagcc catcacagtc agatctatac aacgcttgcc aagttagacc agcagggtta
    14641 tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag atctaccact tgactgatga
    14701 aggacgtgct cttgtagagg aatggatcaa agaggaaact cccgctccta cgcaaaaaga
    14761 tgaatttcta gcaaaaatct atgctttttc gacgctcgat aaaacaacag ctactggact
    14821 gctattcact agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa
    14881 tgacctctcg ctagacaaga aacaagaatt tggacgttat gttgtgatcc agcgcagaat
    14941 tatgctttgt cagcaggaac tccaatggtg tcagcaagta agagaacaaa tgcagcagtt
    15001 ttttgagtaa gggataaaca gaacctttct agaaatatct attgaagagc aaattatctt
    15061 caataaaaga gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta
    15121 tcgttcggat cactattttt ctagaactgc attcaagaga ctatgacaaa aagcatgcca
    15181 aggccttttt tcaaataaca gtgttccatc attcattact ttgaattgct tttagcacac
    15241 ttcttgaaag cgggccaacg atgatcaact gcaacggtaa agcggcaatc acattcattt
    15301 tccaagcatt catataagca gagaataatt gatcaggtat tccttgttcc atgatcacac
    15361 cgaataaaga catacatgtc accattccta aaaccatcaa gcatgaaata gttaagatca
    15421 tttggacctt gctttctgga tttactggta gtttaaaggc aatttttttc gccagcacac
    15481 cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc aggcactaga ccaatcaaca
    15541 aacttgataa atgaagatct tgatgcagcc ataaattata cgcgctcatc cccaagacca
    15601 tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt tttatttgta ggcaatctgt
    15661 accactcctt taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt
    15721 acttttttta ttttctgccc ttttatttca taaacttcat cagccgtacg ctcgacaaag
    15781 taagaatcat gagaagtaaa caaaatcgtt cccggatata tttgcattag cttctctaaa
    15841 gcttcgatcg ttgccatatc aataaaatta gtcggttcat ctaacagcaa cacattactt
    15901 ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact taatgtacat
    15961 aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact acggacaagc
    16021 gattctgaaa actctgtttg tctcatgaca aaacgaatga tggattcttc agatgtcatt
    16081 tgataaccct tttgctgata tacttgaaag cttactttcg gagagagaag gattccttct
    16141 ccttgtttgc ggatatgttc taataaggtt gtcttacctg aaccattttc accgacaatc
    16201 gcgatttttt tacctaaaga aaactgaaaa tcaccattta ccagtaatgt tctcccactt
    16261 ctttccaatt ggacattttg tcccatgatg ggatagcggc tgtggagttc aagagatttt
    16321 ggtactggaa aacggatttg tttcatctgc tgcggcttgg ttgtttctcc tattttttgg
    16381 agtcttctct ctaatgtttt cgcattcttt tggatggctt tttgtacact gtctttttgt
    16441 ttagaggaag acaaccgatc cagtctgatt tgttgctttt tctttttact ggacactttt
    16501 tccaactttt ccgcttgttt tttcttttct tggatcgatt tttccaatcg ttttttctct
    16561 ttcatgatcg actctgcttc tcgtagctga gtctttttct ccaattcctt ttgagtgaga
    16621 taggcactat aattccccga aaactctcgg atttctccgt cttttacttc ccagattttc
    16681 gatgctagct catctagaaa atagcgatca tgactaacaa aaaccagcgt accataataa
    16741 taacgaagct cttcaatcag tcgatcaata ctttttctat ctagatgggt tgttggctcg
    16801 tctagaagca tcccttgttc ataagaagaa agaagacgta tcaactcgat ctttcgttct
    16861 tcccctccgc tccatttccg ctctgaagtt tcttccagtc ttaactggaa atgactggct
    16921 aattccaaat ttaggtcctt tgcttcagcg agataggtta attgaggcag gtagttgaag
    16981 gtgatctctg tttggatttt cccatgatcg ggaaaaagtt caccggctat tagtttgagc
    17041 aaagtcgatt taccgacacc attttctcct atgatcccta tacgatcttg ctgatagaca
    17101 cgaagagaat caatagatag ttctagctgg ttgccaaagc tgacttgtag atttgttata
    17161 tttactgcta aattttccat atgttttaca ctcctatttc atgtaaaaca acaattatcg
    17221 atactaaaaa agagcagaaa aatctgtttg caaaaaaact gcatcacatc ttttcctact
    17281 ctgaacgaaa taactatgac taaatcacca aatctgtcat agctttcacc tattctactg
    17341 tcgttcgaaa aaatggacat actccatcca tgaaattgtt gtttatacat gtcttttgaa
    17401 tttatttagt tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt catttttccg
    17461 acctcctttt ttattaggtt accacacacg attcaaaaaa agcaatagct actgaatagt
    17521 aactgaaaac cttttattcg aacatagtga tcttttctgg tgctaaacga acagactcgt
    17581 aaccttcttc taccgctttt cctattgata aaataataac tggaacataa cggtctttat
    17641 ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc accaattgga tttgtttcat
    17701 agccatgatc acgcgcaact aacatgaatt gcatagccgc taagctagaa tcgactttga
    17761 ctacatcgtt catttcttct cttgaaaaac tcttgtaata aggaatgatc gcagctaatt
    17821 gttgatctct gacttctttt ggcatcttcc ctgattcgta agcttggttg tagatttctt
    17881 cgccatattc gtaacattgc atatcgccaa aaatcatgac cattgcagaa gaagtatcat
    17941 tttgtctcgt attgaaacgg atcaaaggac gcaagatttc ttttgcttcc tcgctttcag
    18001 cgaccacaaa gcgccatggt ttcatattga cagaagaagg tgcagtcgtc gcttctttga
    18061 tcatttccag catttcttcg tgtgggattt tgtatgtttc atcatatacg cgaacggatc
    18121 ttctaccagt cataattgtt gaaaaatcat ttttttgttc cattattatt aacctctttc
    18181 gtttctgttg ttatattgca aagttgggta ttcaatttat ctaaaagtgt tagaaattgt
    18241 ttcatttcta catcgctgaa agccggggat agcacggaac ctgtatcttt atggtccatt
    18301 tcacaatgct tgattttctt ctgggctttt tctgttagcg ctacatacac ttcccggttg
    18361 ttctgaggat ttctctgacg cgaaacatag ccatttgctt ccaatacctt cagatgacga
    18421 gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag caatggattt
    18481 tcttctttca gtctcatcat gatctcgtat ctagtaagac ttataccgat attttcttca
    18541 aagatctgga cgattttttg atcggctaat ttgatttgat ataaaaggtg actaatctct
    18601 tctaaagaca taatttcctc ctttctaata attgactcgt caacgattga cttgtcaata
    18661 atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa
    18721 tagaaaagta tagagaaaag aaattttttg tagaatgaac ggaaaaaatg ataaagaaag
    18781 cacaacgcta aaaccaagat tattgattct tatcaaacta tagaagatag ctcttctgca
    18841 gtagataatt ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa
    18901 tattagaaat tggatcagta tgcttaaaca gtaataaaaa agttatgaca agatgttgtc
    18961 acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata agttcaacca
    19021 aacaaaaaac atgaaaatct gtttttgttt ggctgttctt actacttgat gcagggcagc
    19081 tttttcacta cctcttttta ataaccataa aatcctattc cttcatatct ccagcccatc
    19141 ggtgttagcc aatctctttc cttagcactc aaggtatagt gatgggtacc agttttttca
    19201 tttggattaa atagtcgata aattggcttg gaaccaccag aataaaaact tagaccttca
    19261 taacgccaac cgatttttgt taaagagttg atctcagatt gtgccattgt gtaatgatgg
    19321 tctccactgt aaggattgta taaacggtag acaggttgtc cgctgtttgg tgcttgccaa
    19381 gcaatccctt catattccca gcctacagtt ggcagataat ttttttctcc ggcattcaat
    19441 gtatataaat gttctcctgt atgacggtta tacagacgat acatcggaga agttgaagtt
    19501 ggtgctgtat catactgtgt cagattatag gtttcgatta cattgttcaa tgtagaacca
    19561 taattaggcg cagtggcata acgacctgtt agccaagccg tagcatcttt gtaagagttc
    19621 gtattgcttt tccatgcacc ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc
    19681 aataccgtta cgttatcttg caatgattct ttgtacgaag gatattttct aaaagcggca
    19741 ttcaccgtaa tccattgccc attcacatat tccgaagtag gcatcgtaac agattggcca
    19801 ttatagcttc ctttaatccc aaatagattg taattagggg cagaagccaa agtactgttc
    19861 ccccagccac tttcaacgat ggcttgagca atcatgactg atgcatataa gtcattggca
    19921 cttgccaaac tttgtgcagt tgaggcaata ctatcaatga acgcttgttg agaattagca
    19981 gatgagcgag cataaaaagc atagccagta tagttttcga attcagacaa atctgcttga
    20041 tgggtatgtg tcaatgtttg cccatctgtc aattggttcg tgatttcttc tgcattctct
    20101 ggattgttct gagcttctgc tgatgtactg tcttcggttt gtgtttgttc tgttgtttca
    20161 gttgttggtt cttcggttga aattgtattt tctgagacag tagctgactg caaagtactt
    20221 tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg tctgagttgc gacagtttct
    20281 gtcgattctt cagctagcac tgttgtgttg gcaactgcta gatttcctaa aagaataacg
    20341 ctaaataata aattctttgc tttcatcttt tttctccttt gaattatgaa acttttccta
    20401 aataacagtg taactaaaaa agtaaaagca agtcaatgtc catatttaaa taacatttgt
    20461 ttctttttaa taaatttgct ttttctgcat aaaaagacgt aaatgaagaa tattcttctc
    20521 cattcacgcc ttaaattgat ggtcgtttga ataactattt aacaagtaat tctctctgga
    20581 tattatccgt tatggattat acttcctgtg tcgtcggacg gataatcatg ttgctgacag
    20641 acatccggtc tggcgtttct atcgcaaatg ctacagcaga agctatatct tcagatgtta
    20701 atccccattc tttttgcgct tcatgaagtt caagagcagc ctttttgtcg ctgatggttt
    20761 gatataattc agtttgaaca gctcctggag agatgatcgt agatttgatg ttattttgcc
    20821 gttgctcttg acgtaatcct tccatgattg cacggacagc aaattttgtt ccgcaataaa
    20881 cggcggaatc aggataaaca acatgtcccg ccacagaatc agttgaaatg atatgcccag
    20941 atttttgttt ttccatgatt ggaagtacgg cggaaattcc atttaacaca cccatgatgt
    21001 tgatatcaag catattttgc cattcatcac ggtgaccttc aactaatgga gcggttggca
    21061 tgattcctgc attattgtac aatacatcga ttcgaccgaa cttttccatc gctaaatcga
    21121 taactgcttg gacttgtgca aaatctctta catctgctgt ttgaatatat agctcagctt
    21181 ctggtaaaga ttcttttatt gcttttaggc gatcttctcg gcgtgcagct ataactagtt
    21241 tagccccttt ttctgctaat agcttcgttg ttgcttcacc gattccgctt gaagcgccca
    21301 tgatgacaat cactttttct gataaatcat tcattctatt ctcctcctgt tttgaaaaat
    21361 ctcttgctta tactcactat ctcttatttc atcttactgt tcaaataatc cgttcgacag
    21421 gtttgacgat tttggcaggg attcctgcaa cgatggtatt gtctgggaca tctttcgtaa
    21481 cagttgcatt cgctgcaaca atggcattct cgccaatcgt tacgcctggc agaatagttg
    21541 ctcctgctcc gatccaagca tttttcttga tgtggattgg ctttactgac aagcctcttc
    21601 gttcttttgg atcggttaga tggttcaccg tgatgaggtt gacacgagga ccaatcaata
    21661 ctgaatcttc taaacagatg ccacctaagt caacgaacat cacttgacga ttgataaaga
    21721 catttttccc tatactaata tgtttgccga aatctgtata aaaaggcagc agaacagtca
    21781 ccgtatcatc tactttgtca ctaatgatat tttctagccg tttgcggata tctgctggtt
    21841 catggtaccc agtattcagc gctgctaaag gctcttcgtt ttccgcttgg atctgatgga
    21901 tcacttcaaa gagcggatcc gttactcgaa tctctttatt gacgatttgt ttttcgatga
    21961 ggcttgtcat tttgctccct ccttttatcc ttactttata acggacggct tatgatgtct
    22021 aatacttata aaacatactt aactattcct tttaactata cttagtggta tgctttttta
    22081 aaaacaggag ggaaaaaatg gatattcgta tactgacgta ttttttagca gtagctagag
    22141 aaaaaaatat cagccgagca gcaaaatctc tgcatatcac gcaacctact ctctcaaaac
    22201 aactgaaaga tttagaagaa gaacttggtg tgacactttt cactcgaggc tcccgagaga
    22261 tacagttgac ggattctggt cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg
    22321 tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa atctacattg
    22381 gcggaggcga aactaaagca atctctttca tttctgatag ccttcattcg ttgatcaagg
    22441 cgcatcctga tatccagttt catttatata gcgggaatgc agatgatatc atagacaaac
    22501 tggatagcgg attacttgat tttggactcg taatcgaacc gacaaacaaa caaaaatacc
    22561 agtcatttcg actgccagcc caagatcgtt ggggactttt gatacatcgg aatcatcctt
    22621 tagcaaagca aacagtcatc catccgcagg aactgaaaac tgttcctttg atcatttccc
    22681 ggcaaacgtc agtggacagt caactagctg aatggctagg tttcagcata gatcagttat
    22741 cgatcgtcgg aacatataac ttattgtaca atgcttcttt gatggtcgat aacgggactt
    22801 ttggtgcatt agctctagat gggatcatca atacttatgg gacggaaaaa gtattcatcc
    22861 cactcgatcc gccattacaa gcttcactga atctcatttg gaaaaaagac cagcctcttt
    22921 ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa atacagttaa gtagagtttt
    22981 tttccttcgc tttcagcttt ttaacaacga ataagttaag caaaagatcc aatgattaaa
    23041 taacataagt ttcttaattt gttccttcct tttatttctg ataattccag taaattacta
    23101 taattataaa aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca
    23161 ataattcgtt tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt
    23221 tccctaggag gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg
    23281 tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa
    23341 tgcagtattg gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt
    23401 tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga
    23461 accagaaggt caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt
    23521 tgcagcagaa acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca
    23581 tgtattgatc gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat
    23641 cgtcatccgc aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat
    23701 tgctcaatgg gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga
    23761 agcaggatat atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg
    23821 cagtatccgt gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga
    23881 aacagatatc cattcagaac cattttcact gaattattca agactggtga atcatttacg
    23941 actgcttcta caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga
    24001 aatggtcaaa cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct
    24061 gaccaaagaa taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat
    24121 cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa
    24181 ttgaaagcat atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct
    24241 gctgctgctt tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc
    24301 gctcattttt tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc
    24361 gttgggttag cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg
    24421 gtcgcatttg aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat
    24481 atcaaaccag aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt
    24541 attgccggat tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg
    24601 gctttatctt tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg
    24661 ctgatcagtg cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt
    24721 gctaaattca ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt
    24781 ttactgatcc ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca
    24841 ggaatcaatg atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg
    24901 tatcaagctg gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt
    24961 tacagaaatg cacgtccaga aaacaaagca aaaacagctt ccttgatgtt agcagcagct
    25021 tttgcttcat tctttactgg tgtaacagaa ccacttgaat tctcatttat gttcgttgct
    25081 tggccattat atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc
    25141 ttccattgga cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg
    25201 aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg
    25261 atttactact ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc
    25321 gaggaagcag atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg
    25381 gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat
    25441 aactgtacta cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa
    25501 atcaaagcca ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc
    25561 gtaggtacag aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca
    25621 acacgacctg cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tggaacgatc
    25681 gaacaagata tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat
    25741 gatgtattcg cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa
    25801 atcttctctc caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga
    25861 atccaaacag atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta
    25921 aaaggcgaac cattcacttt atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg
    25981 attgctttag ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt
    26041 gtattcacaa atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa
    26101 gcaaatgata aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt
    26161 ttttatcgat aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa
    26221 taaaaacggc gttctcctgc caactccgcg gtaataagtc cagccaaacg taacatatga
    26281 agagctattt tttatttgac tcttcttatt acttgatgca gaacagcttt tttacaacct
    26341 ctgataaacg gtgttcaaaa accagtttat cggtatgaag tcaaatttat gcaaaaatgt
    26401 ggaaagccat tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc
    26461 tctttataca ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg
    26521 gttttcccaa tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat
    26581 catgttggat cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat
    26641 tttcgacaaa cgctgcatca cttgcttcaa taatttctaa ctttttacct gtcgcacgct
    26701 ctaatgcctt ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg
    26761 gagagtcact ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc
    26821 aacctgtttt tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa
    26881 tgataggtaa ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg
    26941 aatccttaat cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat
    27001 cggcatctgc aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca
    27061 ctacattctt atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat
    27121 ctattccttt caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat
    27181 taaatccagc ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt
    27241 cattttgtgg aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg
    27301 ctcctgttac taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta
    27361 cagctgtaca tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca
    27421 aaaaaaatga aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat
    27481 gtacttgcct atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc
    27541 agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc
    27601 ggtttgatct ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag
    27661 atttctttac tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat
    27721 ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc
    27781 tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg
    27841 atacataaaa tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag
    27901 agatttttat aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa
    27961 ttcttttatt ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca
    28021 ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc
    28081 attagttctg aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc
    28141 aggtcaaagc gctactttca cgacctctat attatcatgg tttattgact accggtatcc
    28201 agatttctcc catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc
    28261 caacataggc gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat
    28321 catttcttaa ttcttcaggc gattctgcta aagcgattac aacaagatac gttccttttg
    28381 ggaatgggat gattcgggta gtgtttggaa catcctgact cgtttgaaca ccgacatagt
    28441 acatcatttt cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt
    28501 gtttcaatgc atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg
    28561 cagccaaacc gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg
    28621 taaaatctgg tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat
    28681 taagttcatc ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga
    28741 tatttttatt ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat
    28801 tactgaaatc ctctccccat tttgtaaaag agatctcggg agcttcgact aactcataat
    28861 ctgaagaagg aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa
    28921 atggcccttt attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat
    28981 cagcgtacgg attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt
    29041 cttccaagcg ggctccatca aatccataag aaacgttgat tacttgattt ggatataaat
    29101 ctcctaactt atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta
    29161 tttcttgatt gactccttca aatttgacag gaactttttt cgccacacct gccacataaa
    29221 actgttcttt cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa
    29281 atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct gatttctgaa ggcagatatc
    29341 ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg
    29401 ccgtttctgt cactcttgat ccatctagga gatcgcgatt agccaaagcc aatctacggt
    29461 ttttgatata gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat
    29521 attcagagat tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt
    29581 cttcgatgta atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgctt
    29641 gcctttttat catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt
    29701 cagatgtaga aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga
    29761 taattggtat tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac
    29821 acattctaag taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact
    29881 acctactgta caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt
    29941 ctttgcgata catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc
    30001 acttcatttt tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt
    30061 cttttacttt ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg
    30121 agcaaaagtg attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata
    30181 ctaaatataa ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt
    30241 tcctgaaaac gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt
    30301 gaactataca agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt
    30361 gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa
    30421 aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc
    30481 caactactca ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg
    30541 ggagctttta acagagattc ttatagaaca tccgcaattt atcgaacgct tcaaagttaa
    30601 gatgccattt catgcaataa attattcaga taaattaaca actatcatcc ataaatacga
    30661 tcatattatc aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa
    30721 gttcatccaa gaagcagttg aatcgctcat tcagcaaaat gcctatcttc ccgcattcga
    30781 attgatcaat gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg
    30841 aacccttctt ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc
    30901 tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc
    30961 ctcttattca aaacgctact ccataaaaat cctgaaagaa gactttcggg aaaaagaatt
    31021 ttctaaccaa aagaaaaaaa tagataaaaa aactaaagaa acggtaaaga aagacgattt
    31081 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
    31141 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
    31201 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
    31261 tcgttgctgg gtagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
    31321 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
    31381 tgtcgaacca ctctaatctt taaagataaa tattaaaata acgattaata atattaacag
    31441 tctttatttt tcaccaccaa aaacctatct ttgcatatac taaatagacg tatatacaca
    31501 gataggtttg tcttgtgaaa aataatgatt atccttacca aaaaatgttt gtttttctac
    31561 atgttcgtac ttatcaaaaa atatgatcct tattgaaatt actggctatt tatattccaa
    31621 cgacttcttg tgttaatttt tgttatctca tagtaaatta tgtttcattt tcacttaatt
    31681 tagttgtcac gtgttcgtca attcgtaaaa acggtaaata tataaacaca cttgcggcta
    31741 taattaatat ttgtaaaaca gctgctcgcc agtctccggc tgttgctaaa aatcccgata
    31801 taactggtgg cgtagtccaa ggaatttgca ctactacatg attaattaat cccacagcag
    31861 ttgcaaaata tgctactgtc aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac
    31921 tgatattaaa tacaataggc aatccaaaaa taataggttc gcttatattg aatatagcgg
    31981 gagttaccga taatttagca aagtccttat aatcttttct acgactgaag ataaatattg
    32041 caatcaataa agcaatcgta tttcccgatc cacccattac cgcaaaggta tctttaaagg
    32101 ccatattgat aatatgtgga atttgctctt tgtttgcata agctgccata ttttcttgca
    32161 tattctgaat caaaaacgga tctaacaatg aacctgatat tactgcttga tggataccaa
    32221 atccaaaaaa tagattagca atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa
    32281 ttttacttaa tggctctgta actatcgtgg taattagctt atttatatcc atattaaaca
    32341 attgaatgat tataaatgac aataaagcaa acgaggtaat cgtaataatg attgggatta
    32401 atacattaaa agactttaaa acagcgggag gaatatttcc ggatagctga atctgtagtt
    32461 tcttattatt agaaagttta ataaataatt ctgtagccgc taatccaaca aatataccta
    32521 caaacattcc tgaagctcct gtataggata taggtatcac acccgtcact tcaacagctt
    32581 tatccgcatt ttcaggagta aacatcatct gcataggtgt taatacaaca aatgtcgaaa
    32641 tagcaactaa taccgctgca atatgatttg tataatttct gcttgcacac atatgataag
    32701 atactgctgc tacaactaat aatgtagtta tgcttaatgt accattggca attgaagtac
    32761 caatttgttg taacgtggtc aatgtactag tatcgacaat ttcccccatg aatcctgttg
    32821 gttctaagat cactgaatta ataagtgtca caaatcctgc taacatcata aatggcataa
    32881 atgtagcaaa cgcatctcgc aaagttctta aatggatctg attccccaac ttagtcgcaa
    32941 ctataccaaa tttttcaaat acaatgtcct tcctgctact tttgcccata ttttttctcc
    33001 tttatgctta ttgtaatcga ttactttttc cacccaaata atacatcact tatcaaaaaa
    33061 agtaaatact tttaattaaa tatatatatc ttttttagta taaaaaaata agctctgaaa
    33121 aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa gtcttgatat
    33181 aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac aactttgtct
    33241 atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa ttcattcgca
    33301 gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc atttgtgata
    33361 taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac tgctaatggc
    33421 atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg tacaatatta
    33481 tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga tggaagttca
    33541 attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct aaacaaacct
    33601 tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc agtcccatac
    33661 ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt ctttactgtt
    33721 aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt ctcaccaatt
    33781 ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat atacttcgca
    33841 atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact ttaacataaa
    33901 taaaccgata aaatccacat caaaattata cacacaataa aaaagatata tatatttaaa
    33961 caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa
    34021 aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt
    34081 ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc
    34141 gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg
    34201 atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct
    34261 ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc
    34321 ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag
    34381 tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg
    34441 aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg
    34501 gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt
    34561 atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat
    34621 tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta
    34681 gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg
    34741 atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg
    34801 ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg
    34861 aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac
    34921 taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc
    34981 catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat
    35041 accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc
    35101 attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac
    35161 gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc
    35221 atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt
    35281 ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt
    35341 cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat
    35401 taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa
    35461 ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac
    35521 tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc
    35581 ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt
    35641 ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag
    35701 tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
    35761 ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt
    35821 ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg
    35881 atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc
    35941 catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg
    36001 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
    36061 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
    36121 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
    36181 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
    36241 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
    36301 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
    36361 gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc
    36421 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
    36481 ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga
    36541 tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
    36601 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
    36661 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
    36721 cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
    36781 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
    36841 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt
    36901 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
    36961 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
    37021 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
    37081 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
    37141 atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta
    37201 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
    37261 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
    37321 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
    37381 atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt
    37441 tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
    37501 ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
    37561 tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg
    37621 cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca
    37681 tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
    37741 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
    37801 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa
    37861 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
    37921 ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt
    37981 catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg
    38041 ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt
    38101 attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt
    38161 gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt
    38221 cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag
    38281 tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat
    38341 agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat
    38401 cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg
    38461 gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta
    38521 cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg
    38581 tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc
    38641 aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta
    38701 tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga
    38761 tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg
    38821 ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg
    38881 ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt
    38941 ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg
    39001 taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt
    39061 ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata
    39121 caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc
    39181 aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct
    39241 ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa
    39301 aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc
    39361 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat
    39421 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
    39481 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa
    39541 atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
    39601 aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt
    39661 acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg
    39721 ttccaaggaa tccgtaacgg aaaccgaaga tcatataaac gatcggattg actttcgaaa
    39781 cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta
    39841 acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc
    39901 cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca
    39961 tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc
    40021 cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag
    40081 gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg
    40141 aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga
    40201 attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa
    40261 taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca
    40321 aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca
    40381 tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga
    40441 ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt
    40501 ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta
    40561 caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt
    40621 tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat
    40681 tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa
    40741 atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat
    40801 tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat
    40861 gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt
    40921 ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg
    40981 cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga
    41041 ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta
    41101 tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg
    41161 attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct
    41221 tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc
    41281 aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct
    41341 ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt
    41401 tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt
    41461 ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt
    41521 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    41581 aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc
    41641 cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct
    41701 gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
    41761 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
    41821 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
    41881 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
    41941 tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
    42001 tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
    42061 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
    42121 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
    42181 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc
    42241 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
    42301 aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact
    42361 tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
    42421 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
    42481 atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct
    42541 cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc
    42601 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
    42661 gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag
    42721 attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga
    42781 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
    42841 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt
    42901 tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca
    42961 aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt
    43021 ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc
    43081 catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat
    43141 ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt
    43201 gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat
    43261 tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg
    43321 cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta
    43381 tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg
    43441 ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag
    43501 gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa
    43561 aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt
    43621 tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa
    43681 atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt
    43741 ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat
    43801 actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat
    43861 ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg
    43921 aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat
    43981 aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat
    44041 taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt
    44101 caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg
    44161 ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt
    44221 gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag
    44281 ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt
    44341 gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt
    44401 ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat
    44461 caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat
    44521 atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat
    44581 ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt
    44641 caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag
    44701 catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga
    44761 agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag
    44821 tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt
    44881 ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa
    44941 tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca
    45001 aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg
    45061 caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta
    45121 ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac
    45181 gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct acataagaag
    45241 gataatctga aataatattg tggaatttac tgtatttatt caacttctct acgacttgat
    45301 caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt
    45361 ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa
    45421 tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg
    45481 cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt
    45541 caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag
    45601 gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat
    45661 atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca
    45721 ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca
    45781 gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag
    45841 tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag
    45901 taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc
    45961 agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc
    46021 tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa
    46081 cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa
    46141 aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag
    46201 accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac
    46261 ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc
    46321 attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc
    46381 aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga
    46441 aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc
    46501 catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag
    46561 ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat
    46621 gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta gactaataac
    46681 ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag ctaatccagc
    46741 aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag tgataaaagc
    46801 tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa tcatcggtac
    46861 gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg tatccgtctg
    46921 gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc ctgctgctac
    46981 gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt ctcttccttg
    47041 tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta attaatgcaa
    47101 ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct actgttaaaa
    47161 tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca aatatgacaa
    47221 catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata gccccctgtg
    47281 tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta gcttttgcca
    47341 tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt gtcatatgaa
    47401 attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc gaatctttta
    47461 ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa atacgtaaat
    47521 gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca actggttgat
    47581 catctaatgc gccccattca acagaccgct ttaacttggc tacagccact gttgattcaa
    47641 ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct gtggtcatat
    47701 ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca aacccattag
    47761 tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca tttaaatcta
    47821 tcactatatt atttatattg attttttcac tgatcattta taagccttct ttcaatatat
    47881 aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca tgattcgaag
    47941 cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt tcattaagtg
    48001 caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct ggattaacgt
    48061 agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata gcaaaatcat
    48121 tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc ccttctttcg
    48181 cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact tgattatcct
    48241 ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa gcatcttctg
    48301 tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct aacatcaaag
    48361 gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt
    48421 catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct atctcaacca
    48481 tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca tatccgctac
    48541 tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt gattgaatat
    48601 ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc tcttcgattt
    48661 cctctaataa atctacactt atcataaatg accttttaaa agaatatttg attttgtccg
    48721 tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca ttgagatgta
    48781 atactagatt attgatcaat tcttcatcag gataaaattc atttaattta attcttacta
    48841 actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat
    48901 tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg
    48961 gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga
    49021 tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg
    49081 caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct
    49141 tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat
    49201 tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg
    49261 ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt
    49321 catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta
    49381 gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt
    49441 cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag
    49501 ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa
    49561 gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat
    49621 ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag
    49681 atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt
    49741 atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac
    49801 agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta
    49861 gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcctgcaat atttatatca
    49921 cag
//
LOCUS       DAYYHB010000013        47051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000013 DAYYHB010000000
VERSION     DAYYHB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.013"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHA6B_000663"
     CDS             complement(53..787)
                     /locus_tag="ACHA6B_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8948261.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHA6B_000664"
     CDS             1114..1524
                     /locus_tag="ACHA6B_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8948262.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHA6B_000665"
     CDS             1517..2218
                     /locus_tag="ACHA6B_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8948263.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHA6B_000666"
     CDS             2218..3804
                     /locus_tag="ACHA6B_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948264.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHA6B_000667"
     CDS             3823..4044
                     /locus_tag="ACHA6B_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8948265.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHA6B_000668"
     CDS             4041..4802
                     /locus_tag="ACHA6B_000668"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8948266.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHA6B_000669"
     CDS             5182..5691
                     /locus_tag="ACHA6B_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8948267.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHA6B_000670"
     CDS             complement(5766..6305)
                     /locus_tag="ACHA6B_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8948268.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHA6B_000671"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHA6B_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8948269.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHA6B_000672"
     CDS             7318..8169
                     /locus_tag="ACHA6B_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8948270.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHA6B_000673"
     CDS             8197..8832
                     /locus_tag="ACHA6B_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8948271.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHA6B_000674"
     CDS             8852..9625
                     /locus_tag="ACHA6B_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8948272.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHA6B_000675"
     CDS             10033..10830
                     /locus_tag="ACHA6B_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8948273.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHA6B_000676"
     CDS             10808..11221
                     /locus_tag="ACHA6B_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8948274.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHA6B_000677"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHA6B_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8948275.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHA6B_000678"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHA6B_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8948276.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHA6B_000679"
     CDS             15998..16558
                     /locus_tag="ACHA6B_000679"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8948277.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHA6B_000680"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHA6B_000680"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8948278.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHA6B_000681"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHA6B_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8948279.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHA6B_000682"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHA6B_000682"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8948280.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHA6B_000683"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHA6B_000683"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8948281.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNEKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHA6B_000684"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHA6B_000684"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8948282.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHA6B_000685"
     CDS             21905..22483
                     /locus_tag="ACHA6B_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948283.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHA6B_000686"
     CDS             22535..23017
                     /locus_tag="ACHA6B_000686"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8948284.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHA6B_000687"
     CDS             23134..23508
                     /locus_tag="ACHA6B_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948285.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="ACHA6B_000688"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="ACHA6B_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8948286.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="ACHA6B_000689"
     CDS             25685..26275
                     /locus_tag="ACHA6B_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8948287.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="ACHA6B_000690"
     CDS             complement(26403..27752)
                     /locus_tag="ACHA6B_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8948288.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="ACHA6B_000691"
     CDS             complement(27920..28660)
                     /locus_tag="ACHA6B_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8948289.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="ACHA6B_000692"
     CDS             complement(28673..30265)
                     /locus_tag="ACHA6B_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8948290.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(30403..30577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(30833..31129)
                     /locus_tag="ACHA6B_000693"
     CDS             complement(30833..31129)
                     /locus_tag="ACHA6B_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948291.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="ACHA6B_000694"
     CDS             31305..32030
                     /locus_tag="ACHA6B_000694"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8948292.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="ACHA6B_000695"
     CDS             32023..32865
                     /locus_tag="ACHA6B_000695"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8948293.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="ACHA6B_000696"
     CDS             32868..33389
                     /locus_tag="ACHA6B_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8948294.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /gene="def"
                     /locus_tag="ACHA6B_000697"
     CDS             complement(33642..34205)
                     /gene="def"
                     /locus_tag="ACHA6B_000697"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8948295.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="ACHA6B_000698"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="ACHA6B_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8948296.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="ACHA6B_000699"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="ACHA6B_000699"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8948297.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="ACHA6B_000700"
     CDS             37549..37815
                     /locus_tag="ACHA6B_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8948298.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39950
                     /locus_tag="ACHA6B_000701"
     CDS             37959..39950
                     /locus_tag="ACHA6B_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8948299.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="ACHA6B_000702"
     CDS             40304..41605
                     /locus_tag="ACHA6B_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8948300.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="ACHA6B_000703"
     CDS             complement(41735..43105)
                     /locus_tag="ACHA6B_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8948301.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="ACHA6B_000704"
     CDS             complement(43287..43610)
                     /locus_tag="ACHA6B_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8948302.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43935..44543)
                     /locus_tag="ACHA6B_000705"
     CDS             complement(43935..44543)
                     /locus_tag="ACHA6B_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8948303.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44873..45589
                     /locus_tag="ACHA6B_000706"
     CDS             44873..45589
                     /locus_tag="ACHA6B_000706"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8948304.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45602..46297
                     /locus_tag="ACHA6B_000707"
     CDS             45602..46297
                     /locus_tag="ACHA6B_000707"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8948305.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46381..47010
                     /gene="udk"
                     /locus_tag="ACHA6B_000708"
     CDS             46381..47010
                     /gene="udk"
                     /locus_tag="ACHA6B_000708"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8948306.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15260 a         8719 c         9791 g        13281 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agttcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacaaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atgaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcaataga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgac gaaaataaaa taacaaacaa tttgtaaagt tgacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa aatttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcgaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacgattat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttt cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatggctggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggcta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa agtataaatt tttataaagg tacgataaaa gtatgcgagg aaaagcttgg
    43801 taaattagga tttttcgttt gtaaagccat ttacaaaagt ggaagcccca tatgaaaata
    43861 aacgattaga aataaaaaag gcgtgttaaa agctgctgac ccgccgcttc taacaccacc
    43921 tgattgtttc agtattattc ttctgttgaa ccgatcactt ctggttcgcc accttcacct
    43981 gtgctttctt cttcttcact ttgagcgact acagcagcga tttgctcttc actgtcggtc
    44041 acgaatttgt aatcgtcgtt ttttggaaga tcggctactg taatagaatc acctaattcc
    44101 aagtttgtca cgtcaacttc aattcgttca ggcaatttgt ctggtgtagc agatacgact
    44161 actgtataca agttttgagc taacactcca ccagatttag cacccactga ttcgccaaca
    44221 agaacgactt ctgtttcgac ttctgtttct tccgacatgt ttacagatag aaattctgca
    44281 tgttcgaaat gattggtaaa agtgttcgtt tggatcttgt gaaccaatgt attgactttt
    44341 tgtccatcga tgtccatcgt gatgactgtg ttcgctccat gttcacgcaa caatttgctt
    44401 agtgtcaaac catcgaatga gataggggta ctttctactt tatacccata aacaacagct
    44461 ggaactttcc cttcgtgacg aagtttgttt cttaatgaac ggggacgtac ttctctttta
    44521 cttacttcta atgatactga cataaccaaa attcctcctt aaaatgtgac tgtctgcttt
    44581 ggtaacggac agattttatt taagtgaatt ctaaaggcta atctcgtgca catcatcgaa
    44641 ggcagggcgc ttaaaaaagc gcaaaaggag agatgcgcgc atctcccctt agaaagtcag
    44701 gattcaactt ttctccacca ggtcattacg ctgtgtatga gaaatccaat tcactttact
    44761 atttaaccgt aacgtgaact gttaattaag tcaaggaata actatgatga tgtatttgca
    44821 agcaagaata ttcaaggtat aattaattag tagtaataag aaaggaaaca gcatgcgttt
    44881 agataaatta atagaagaaa aattagaaac gacacgcaag gaaatgaagc ggctgtttgc
    44941 tttgaagcaa gtgatgatag atggcacagt cgaatttcga caaaatcgaa atgtagatag
    45001 tttactgcat caaatcgaag tagcaggtga acacttagaa acaaatgagt cctattatct
    45061 tctcaataaa cctgaaaaag ctgttacagc tgtatcagat ccagaaaaga cgacagtaat
    45121 cgatctgatt gcaagtgccg atcgtttcca accattatat ccagtgggaa gactagatag
    45181 agatacaaca ggactattgc tgatcacttc gaatggacaa ttgggatatg aaatgctact
    45241 tccagaaaaa aaggtttcaa agacttatca agtagttgtg aacgaaaggg tgacaccggc
    45301 agacgtaaca gcatttgaaa atggaatcgt attcgctgat gggtatcaat gtaagccggc
    45361 tgatttaaaa atactcaaag cagacaagca ccaaagtacg gttttattga cgatccaaga
    45421 aggaaaattc catcaagtca aaaagatgtt cttagcccaa ggaaagaaag tgaccgcatt
    45481 gaaacggcta agcatgggac cattggaatt agatgaacag ctaggagtag gcgagtatcg
    45541 atcgttgaca ttagcagaga tgaaaaagtt aagtatttat tttaaatagg aggtcaaaaa
    45601 gatgaaatat aaaactttat tgtttgatgt agatgataca ttgttagatt ttcaattaac
    45661 tgaagccaat gctttaagtg ctttgtttgc agaacaagga atcacattga caccagaaat
    45721 cgaaaccagc tataaggctt tgaatcatca tttatggcag gagtttgaaa aaggcagact
    45781 atcacgagaa gaaataatag gcagtcgttt tgggttactt ttccagcaat ttgggaaaac
    45841 agtagatagt gatcaaatgg aaaaacgcta ccgccactac ttgaaccaag gtcatgatct
    45901 tgttccagga agtctagaaa tcctgaaaaa ggtccatccg catgctgatc tttatattgt
    45961 gacgaacggt gtgtcgcaca cgcaacatcg gcgattaaca gatgcaggaa tgcttgatta
    46021 cttcaaggat atctttgtct cagaagctgt cggagcgcaa aagccgatga aagaattttt
    46081 tgatcatgtc ttttcccaca ttcctaattt tgataaaaag caaactgtga tcattggtga
    46141 ttctttgact tctgatatca aaggaggaaa tatggcaggg atcgatacga tttggttcaa
    46201 tagaaataaa ttaccagaaa tcgaagaaat ccagcctact tatcgaatcg attcattaga
    46261 ggaactttat tcattattgg aaattgaaaa ggattaattt tcagaaaaat atagcatttt
    46321 cgtgtttttt cgctataata gattagaatg aaaaaacgga tacgaaagag gttttagtca
    46381 atgacaaaaa gcaagccaat tatcattggt gtgacgggtg gatccggaag cggaaagaca
    46441 agtgtcagcc gtgcgatttt caacaacttc ccggaccatt ccatcatgat gcttgaacaa
    46501 gattcttatt acaaggatca gagccatttg agttttgaag aacgattgaa cacaaattac
    46561 gatcacccat ttgcctttga taatgatctg ctgattcagc atgtaggcga cttactgaac
    46621 tataaagcca ttgaaaaacc cgtgtatgac tatgtagcgc atacaagaag tcaggcaacg
    46681 atcattcagg aaccaaaaga agtgatcatt ctagaaggaa tcctgatttt agaagacgaa
    46741 cgtctgcgtg acttgatgga tatcaaagta tacgtagata cagatgatga cattcgtatc
    46801 attcgtcgta tcaaacggga tatggaagaa cggggacgca cattggactc cgtgatcgaa
    46861 caatatttga cagttgttaa accgatgtat catcaattta tcgaaccaac gaagcgctat
    46921 gcggatatca ttgttccaga aggcggagaa aaccacgttg ctattgattt gattacaaca
    46981 aaagtagcta gtttcttaaa ccataaatag tgcgcagcga aacagttgaa cggactgcga
    47041 ccgtttgaaa a
//
LOCUS       DAYYHB010000014        47009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000014 DAYYHB010000000
VERSION     DAYYHB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.014"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..665)
                     /locus_tag="ACHA6B_000709"
     CDS             complement(138..665)
                     /locus_tag="ACHA6B_000709"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8948307.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(732..2234)
                     /gene="eat(A)"
                     /locus_tag="ACHA6B_000710"
     CDS             complement(732..2234)
                     /gene="eat(A)"
                     /locus_tag="ACHA6B_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8948308.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2492..3613)
                     /locus_tag="ACHA6B_000711"
     CDS             complement(2492..3613)
                     /locus_tag="ACHA6B_000711"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8948309.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3739..4680
                     /locus_tag="ACHA6B_000712"
     CDS             3739..4680
                     /locus_tag="ACHA6B_000712"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8948310.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4830..5726)
                     /locus_tag="ACHA6B_000713"
     CDS             complement(4830..5726)
                     /locus_tag="ACHA6B_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8948311.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5995..7335
                     /locus_tag="ACHA6B_000714"
     CDS             5995..7335
                     /locus_tag="ACHA6B_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8948312.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7553..7996
                     /locus_tag="ACHA6B_000715"
     CDS             7553..7996
                     /locus_tag="ACHA6B_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8948313.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8500..9918)
                     /gene="nhaC"
                     /locus_tag="ACHA6B_000716"
     CDS             complement(8500..9918)
                     /gene="nhaC"
                     /locus_tag="ACHA6B_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8948314.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10321..11544)
                     /locus_tag="ACHA6B_000717"
     CDS             complement(10321..11544)
                     /locus_tag="ACHA6B_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8948315.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11575..12414)
                     /locus_tag="ACHA6B_000718"
     CDS             complement(11575..12414)
                     /locus_tag="ACHA6B_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8948316.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12431..13111)
                     /locus_tag="ACHA6B_000719"
     CDS             complement(12431..13111)
                     /locus_tag="ACHA6B_000719"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8948317.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13113..14186)
                     /locus_tag="ACHA6B_000720"
     CDS             complement(13113..14186)
                     /locus_tag="ACHA6B_000720"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8948318.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14242..14480)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14523..14756)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14833..15504)
                     /locus_tag="ACHA6B_000721"
     CDS             complement(14833..15504)
                     /locus_tag="ACHA6B_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8948319.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15591..18239)
                     /gene="mgtA"
                     /locus_tag="ACHA6B_000722"
     CDS             complement(15591..18239)
                     /gene="mgtA"
                     /locus_tag="ACHA6B_000722"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8948320.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18581..18755)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18928..19365
                     /gene="ndk"
                     /locus_tag="ACHA6B_000723"
     CDS             18928..19365
                     /gene="ndk"
                     /locus_tag="ACHA6B_000723"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8948321.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19387..20253)
                     /locus_tag="ACHA6B_000724"
     CDS             complement(19387..20253)
                     /locus_tag="ACHA6B_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8948322.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20413..22506)
                     /locus_tag="ACHA6B_000725"
     CDS             complement(20413..22506)
                     /locus_tag="ACHA6B_000725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8948323.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23255..23878)
                     /locus_tag="ACHA6B_000726"
     CDS             complement(23255..23878)
                     /locus_tag="ACHA6B_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948324.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24037..25434)
                     /locus_tag="ACHA6B_000727"
     CDS             complement(24037..25434)
                     /locus_tag="ACHA6B_000727"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8948325.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25466..26437)
                     /locus_tag="ACHA6B_000728"
     CDS             complement(25466..26437)
                     /locus_tag="ACHA6B_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8948326.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26811..27236
                     /locus_tag="ACHA6B_000729"
     CDS             26811..27236
                     /locus_tag="ACHA6B_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948327.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27724..28425)
                     /locus_tag="ACHA6B_000730"
     CDS             complement(27724..28425)
                     /locus_tag="ACHA6B_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8948328.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28441..28881)
                     /locus_tag="ACHA6B_000731"
     CDS             complement(28441..28881)
                     /locus_tag="ACHA6B_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8948329.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28881..29456)
                     /locus_tag="ACHA6B_000732"
     CDS             complement(28881..29456)
                     /locus_tag="ACHA6B_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8948330.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            29697..29906
                     /locus_tag="ACHA6B_000733"
     CDS             29697..29906
                     /locus_tag="ACHA6B_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8948331.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(30304..30816)
                     /locus_tag="ACHA6B_000734"
                     /pseudo
     CDS             complement(30304..30816)
                     /locus_tag="ACHA6B_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30942..31643)
                     /locus_tag="ACHA6B_000735"
     CDS             complement(30942..31643)
                     /locus_tag="ACHA6B_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8948332.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31751..33194)
                     /locus_tag="ACHA6B_000736"
                     /pseudo
     CDS             complement(31751..33194)
                     /locus_tag="ACHA6B_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(33208..35073)
                     /locus_tag="ACHA6B_000737"
     CDS             complement(33208..35073)
                     /locus_tag="ACHA6B_000737"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8948333.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(35194..36044)
                     /gene="licT"
                     /locus_tag="ACHA6B_000738"
                     /pseudo
     CDS             complement(35194..36044)
                     /gene="licT"
                     /locus_tag="ACHA6B_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(36354..37067)
                     /locus_tag="ACHA6B_000739"
     CDS             complement(36354..37067)
                     /locus_tag="ACHA6B_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8948334.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(37135..37461)
                     /locus_tag="ACHA6B_000740"
     CDS             complement(37135..37461)
                     /locus_tag="ACHA6B_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8948335.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37584..37811)
                     /locus_tag="ACHA6B_000741"
     CDS             complement(37584..37811)
                     /locus_tag="ACHA6B_000741"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8948336.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(38133..39386)
                     /locus_tag="ACHA6B_000742"
     CDS             complement(38133..39386)
                     /locus_tag="ACHA6B_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8948337.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            39447..40187
                     /locus_tag="ACHA6B_000743"
     CDS             39447..40187
                     /locus_tag="ACHA6B_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8948338.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(40273..41031)
                     /locus_tag="ACHA6B_000744"
     CDS             complement(40273..41031)
                     /locus_tag="ACHA6B_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8948339.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(41028..41492)
                     /locus_tag="ACHA6B_000745"
                     /pseudo
     CDS             complement(41028..41492)
                     /locus_tag="ACHA6B_000745"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(41511..42008)
                     /locus_tag="ACHA6B_000746"
     CDS             complement(41511..42008)
                     /locus_tag="ACHA6B_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8948340.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(42076..45243)
                     /locus_tag="ACHA6B_000747"
     CDS             complement(42076..45243)
                     /locus_tag="ACHA6B_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8948341.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(45467..46351)
                     /locus_tag="ACHA6B_000748"
     CDS             complement(45467..46351)
                     /locus_tag="ACHA6B_000748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8948342.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(46446..46883)
                     /locus_tag="ACHA6B_000749"
     CDS             complement(46446..46883)
                     /locus_tag="ACHA6B_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948343.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        13465 a         9866 c         8030 g        15648 t
ORIGIN      
        1 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
       61 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
      121 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
      181 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
      241 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
      301 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
      361 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
      421 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
      481 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
      541 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
      601 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
      661 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
      721 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
      781 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
      841 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
      901 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
      961 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
     1021 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
     1081 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
     1141 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
     1201 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
     1261 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
     1321 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
     1381 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
     1441 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
     1501 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
     1561 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
     1621 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
     1681 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
     1741 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
     1801 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
     1861 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
     1921 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
     1981 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
     2041 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
     2101 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
     2161 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
     2221 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
     2281 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
     2341 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
     2401 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
     2461 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
     2521 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
     2581 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
     2641 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
     2701 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
     2761 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
     2821 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
     2881 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
     2941 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
     3001 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
     3061 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
     3121 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
     3181 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
     3241 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
     3301 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
     3361 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
     3421 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
     3481 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
     3541 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
     3601 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
     3661 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
     3721 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
     3781 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
     3841 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
     3901 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
     3961 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
     4021 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
     4081 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
     4141 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
     4201 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
     4261 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
     4321 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
     4381 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
     4441 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
     4501 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
     4561 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
     4621 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
     4681 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
     4741 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
     4801 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
     4861 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
     4921 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
     4981 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
     5041 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
     5101 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
     5161 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
     5221 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
     5281 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
     5341 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
     5401 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
     5461 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
     5521 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
     5581 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
     5641 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
     5701 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
     5761 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
     5821 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
     5881 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
     5941 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
     6001 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
     6061 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
     6121 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
     6181 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
     6241 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
     6301 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
     6361 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
     6421 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
     6481 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
     6541 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
     6601 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
     6661 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
     6721 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
     6781 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
     6841 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
     6901 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
     6961 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
     7021 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
     7081 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
     7141 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
     7201 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
     7261 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
     7321 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
     7381 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
     7441 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
     7501 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
     7561 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
     7621 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
     7681 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
     7741 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
     7801 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
     7861 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
     7921 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
     7981 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
     8041 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
     8101 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
     8161 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
     8221 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
     8281 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
     8341 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
     8401 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
     8461 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
     8521 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
     8581 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
     8641 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
     8701 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
     8761 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
     8821 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
     8881 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
     8941 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
     9001 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
     9061 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
     9121 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
     9181 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
     9241 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
     9301 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
     9361 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
     9421 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
     9481 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
     9541 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
     9601 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
     9661 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
     9721 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
     9781 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
     9841 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
     9901 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
     9961 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    10021 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    10081 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    10141 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    10201 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    10261 atattttttc atttttctga aaattatttt tataactttc acacacaagt ttatttttta
    10321 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    10381 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    10441 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    10501 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    10561 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    10621 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    10681 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    10741 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    10801 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    10861 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    10921 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    10981 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    11041 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    11101 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    11161 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    11221 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    11281 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    11341 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    11401 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    11461 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    11521 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    11581 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    11641 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    11701 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    11761 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    11821 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    11881 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    11941 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    12001 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    12061 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    12121 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    12181 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    12241 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    12301 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    12361 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    12421 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    12481 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    12541 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    12601 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    12661 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    12721 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    12781 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    12841 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    12901 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    12961 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    13021 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    13081 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    13141 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    13201 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    13261 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    13321 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    13381 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    13441 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    13501 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    13561 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    13621 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    13681 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    13741 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    13801 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    13861 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    13921 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    13981 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    14041 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    14101 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    14161 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    14221 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    14281 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    14341 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    14401 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    14461 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    14521 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    14581 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    14641 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    14701 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    14761 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    14821 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    14881 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    14941 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    15001 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    15061 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    15121 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    15181 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    15241 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    15301 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    15361 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    15421 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    15481 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    15541 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    15601 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    15661 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    15721 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    15781 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    15841 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    15901 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    15961 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    16021 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    16081 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    16141 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    16201 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    16261 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    16321 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    16381 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    16441 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    16501 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    16561 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    16621 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    16681 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    16741 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    16801 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    16861 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    16921 gcgccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    16981 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    17041 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    17101 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    17161 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    17221 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    17281 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    17341 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    17401 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    17461 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    17521 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    17581 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    17641 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    17701 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    17761 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    17821 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    17881 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    17941 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    18001 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    18061 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    18121 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    18181 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    18241 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    18301 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    18361 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    18421 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    18481 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    18541 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    18601 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    18661 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    18721 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    18781 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    18841 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    18901 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    18961 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    19021 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    19081 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    19141 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    19201 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    19261 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    19321 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    19381 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    19441 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    19501 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    19561 cactgtactt ctttctttat gtgataagct ccgtttcata tcttcattca cactacgtgc
    19621 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    19681 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    19741 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    19801 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    19861 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    19921 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    19981 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    20041 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    20101 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    20161 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    20221 tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt
    20281 ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat
    20341 gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt
    20401 tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata
    20461 taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt
    20521 ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc
    20581 aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc
    20641 tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc
    20701 tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac
    20761 ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga
    20821 agataataag tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag
    20881 taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata
    20941 accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca
    21001 tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc
    21061 aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata
    21121 cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac
    21181 tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg
    21241 cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata
    21301 ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga
    21361 ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc
    21421 tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg
    21481 tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga
    21541 gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc
    21601 ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact
    21661 atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa
    21721 actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt
    21781 tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc
    21841 atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc
    21901 gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg
    21961 gttggcttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc
    22021 gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca
    22081 aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat
    22141 caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt
    22201 gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt
    22261 atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa
    22321 agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg
    22381 gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca
    22441 gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg
    22501 tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa
    22561 aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc
    22621 cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag
    22681 tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa
    22741 aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt
    22801 tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat
    22861 atttataaaa catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg
    22921 caggctcaag acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg
    22981 tcaggacgct aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    23041 ataagcccaa ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc
    23101 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg
    23161 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    23221 caggtcaaaa cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc
    23281 cttgtgatga ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt
    23341 gtccactgcc agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag
    23401 ttgttttctt gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt
    23461 ttttgtctac tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt
    23521 tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc
    23581 tgagagaaat cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt
    23641 ttggcaaata aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc
    23701 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    23761 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    23821 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    23881 tttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    23941 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat
    24001 ggagaatttc acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc
    24061 tgatacgtct ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa
    24121 gtctactgaa ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat
    24181 acatgtccac atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata
    24241 tttcagatga gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt
    24301 gataaatcgt gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt
    24361 tccataatga tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca
    24421 tccgcgataa ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg
    24481 cattggaccg tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta
    24541 ataattgata cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg
    24601 gatcacaggc atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc
    24661 ttttacagct ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt
    24721 aacatcttca gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc
    24781 aataacacca ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc
    24841 ttcatgatag ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg
    24901 atataaataa cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac
    24961 tttcgaacca aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc
    25021 tcggttcaac catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt
    25081 cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac
    25141 ggcttcttcg ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt
    25201 tcgaaatgaa ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata
    25261 aacttgcgag gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta
    25321 ccaataatca aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg
    25381 gccacttgct gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc
    25441 acctcttttt cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa
    25501 tcactttttc attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc
    25561 gttccccgat tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt
    25621 ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt
    25681 gattgaaaca ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg
    25741 cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg
    25801 taaagacctc taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac
    25861 gttctcttgc tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt
    25921 gttcaccatg atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat
    25981 caatacctgc tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga
    26041 gcatttcaaa tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat
    26101 gcttcatcag gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta
    26161 ataataagct atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc
    26221 caaaacggct ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca
    26281 accctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt
    26341 tcatgttctt ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa
    26401 caccagccat cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac
    26461 atttgatttc tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct
    26521 tgtcatcaat atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt
    26581 atacttaaac aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc
    26641 aacacttttt taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt
    26701 ctaaaattta ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt
    26761 ttacattcca atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat
    26821 taatcgtact atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg
    26881 gcggctctga ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag
    26941 cagataccac caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg
    27001 gacagttgac atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa
    27061 ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca
    27121 aagatgacga atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta
    27181 caaaaatcgg taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc
    27241 gtctgttgga tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg
    27301 attgtaaagg agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac
    27361 aaaaatatcc atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac
    27421 aaactagtca aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa
    27481 agtagaagaa gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca
    27541 aactcagctt cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt
    27601 ttacaaatca aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac
    27661 gatagaaagg cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta
    27721 tttttagcct aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg
    27781 ctttccttcc aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc
    27841 tggattttct acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa
    27901 atcttcatac cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc
    27961 atcgctttct agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc
    28021 caaagaaact ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa
    28081 ctcgtttagc atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg
    28141 acctaaatca gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac
    28201 attgtcatcg ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat
    28261 ttgcaataaa aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc
    28321 ttcgacattt tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact
    28381 gctcttacct gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt
    28441 ctataagtac tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac
    28501 agcttggtct gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac
    28561 cgtctcttct tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta
    28621 agccgttcct ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc
    28681 atttgtcagt tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag
    28741 ttcataattg ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc
    28801 ctccaacgct ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc
    28861 tgctaaatat atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat
    28921 actgctcgta attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac
    28981 cattttttat ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg
    29041 taataaccgc caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc
    29101 caagaaagaa gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca
    29161 gcatctccct tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat
    29221 gtagtaacat cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg
    29281 acttttccgt tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa
    29341 ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata
    29401 aatttgtaac tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa
    29461 aaacactccc taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga
    29521 ctagattttg tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga
    29581 aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg
    29641 atatgaaatg acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt
    29701 attattttaa acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt
    29761 gttatgtttc actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc
    29821 aaaatttgat catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata
    29881 gctggatttg taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc
    29941 gcacctgttg cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct
    30001 ctagcatgaa gaataatttc tttttttact gtataataac tttctttcct ttttggaata
    30061 gtacaggaaa gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac
    30121 aacgaaaggt tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac
    30181 agtttcgatc gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt
    30241 cataccattc ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct
    30301 ctcctaacta attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat
    30361 gatttctgca ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa
    30421 agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa
    30481 cattcccgat gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac
    30541 tgtcccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa
    30601 acatccctct gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc
    30661 ttgacgattg agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc
    30721 gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga
    30781 aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg
    30841 cggtaataag cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    30901 tacttgatgc agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata
    30961 tggtaaggat gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt
    31021 tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt cagaattcct
    31081 tgttcttctt ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc aatagtatat
    31141 ccttttttcg tcagcaaacg atacaaagat tctttgccaa attcttctct gctgaattgc
    31201 atcatttctt gtggaagata atgaacaaaa taaatataag gctgattgtt caaataatat
    31261 aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc ttccttcttt
    31321 atatggacca attgcaaatc cagcgtttct ttccggactt catatcggga gttctccaag
    31381 atttgcgtaa agcttgccgc tttggataat cgattgtaag gacgattgct tagaaccgtc
    31441 gtcccacgac cacttttctt tgcgacatat tcatctgatg ccagcagttc gattgccttt
    31501 cggacagtga ttttgctgac ttgaaacagt tcttctaatt cactttctgt cggcaacatc
    31561 gaaccgactg gatattttcc tgagaaaata tcttctttaa ttttatctgc gacttccaag
    31621 tatagtacac gtgtcctttt cataacgtca ccctttgctt tactattact aaaagaatag
    31681 cacataaaaa ataaaaaaag aacacaaatc acatgtgaga agtgtgttct ttttcattca
    31741 gctgctttaa ttacaagttt acgccatttg aggcaattac ttctttatac caatcaaaag
    31801 attttttctt tgatcgattc agtgtcccat ttccttcatc gtctagatcc acatagataa
    31861 agccgtaacg tttactcatt tctcctgttg atgcactgac aagatcgata catccccaag
    31921 gcgtatagcc catcaaatct acaccgtctg ctactgcatt agccatcgct tcgatatgac
    31981 gacgtaaata atcgatccga taatcatcaa taatcgtgcc atcctcttcc atatgatcaa
    32041 tggctccaag accgttttca acaatgaata atggtttctg gtaacgattg tataattcat
    32101 tcaatgcgat tctcagccct tctggatcga tctgccagcc ccattcactt gcctcaagaa
    32161 acggattttt tactcctccc agcaagttcc cttctgaagt tgctgcttgt ggatcagtct
    32221 cattgattgc cgtagacata taataactaa atccgatgta gtccactgga tattctttta
    32281 gcaactggaa gtctttttcc gtttgctcta atttttccgg atcgactcca tattttttat
    32341 gggtacgcgc tgtgtatgca gggtattctc cacgtacttg gacatccgtg cagaaaaaat
    32401 tgaattcttg gttttggatc attgttgctg cctgattgac tggattggca tcgatgctgt
    32461 aagttgttgc atagataatc atgcagccga tttggatatc cgaacgcaat tcgtggccga
    32521 tttttacggc tttgctactt gcaacgaatt gattatgcca tgcttggaaa atattttggt
    32581 actctcctgc tccattgctt tttactaatc cttgacttaa agctggaaag tgaaaggccg
    32641 aattgatttc attgaaagtc atccaatatt tgactttact gctgtatctc tctaatactg
    32701 tctgagcaaa tcgttcatag aagtcgatca gttttcgatt tttccagcca ccgtattctt
    32761 ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat cacaggttca atatcatatt
    32821 ttaagcactc atcaatcagc tgatcataaa attctaatcc cgcttcgtta ggagtacctt
    32881 cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa acgataacat ttgaatccca
    32941 tttccgcaaa caaggcaaga tcttctttga aacgatcgta atgatcgatt cctcgatgat
    33001 tcgggtaaat atatttttct tgatcaatcg tccagtcaaa ttcttcactg ccgataatcg
    33061 caaagcgctg cttacctcca ggcatagcat ctgcaacgga tagtcctttt ccgtctcgat
    33121 cataagcccc ttctaattga tttggcagca gttgctccac cccataaaaa tttttctgaa
    33181 aaacgtgatt gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc
    33241 aataatctct ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac
    33301 aactggagtg attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa
    33361 taaatctcct gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt
    33421 taattctact gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat
    33481 ccctacagca tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag
    33541 ttctcctact tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg
    33601 gtctggtact tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg
    33661 tgctgaagta atcggttctc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt
    33721 ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt
    33781 agctattact gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg
    33841 aatcgtcaat aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat
    33901 agctccacca atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt
    33961 cacaccatac acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc
    34021 taatgctcga agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg
    34081 tgccaagatg gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt
    34141 caaaaacatg atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa
    34201 acttcccatg attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc
    34261 gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac
    34321 catcactact aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt
    34381 ttttacgaat ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga
    34441 agaagtataa ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc
    34501 cgcaccagca actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa
    34561 aaactggttg gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt
    34621 aaagacacca tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca
    34681 gcccatcacc gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc
    34741 tagaaaaggt gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga
    34801 ctcttctttt ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac
    34861 atctgctaca ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat
    34921 cacgtccggg tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa
    34981 tcgcaaccgc gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc
    35041 ccatacacga cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt
    35101 aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca
    35161 atccaggttt tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact
    35221 tactactctt tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt
    35281 ttgcgtttgt cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt
    35341 tccttgaact aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata
    35401 acgctgggca aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc
    35461 gaacacgatg ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc
    35521 ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat
    35581 aaaaccggct tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc
    35641 ctctttcgct gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt
    35701 aggcagttgg atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt
    35761 cagataaata ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg
    35821 tttgacccat tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt
    35881 agcaaaagaa tctcttaaac aaattctttt tggaccgctt ctggcgaaat ctcgtcccca
    35941 atcttttttt gaaaagctag tccttttccc atacaaacga cttcttcccc gaatccattt
    36001 ttcgaaatga cgacattgtt attcaaaatt ttatcaattt tcattttgct tcgccctctc
    36061 aagaaaaata aaaaaaccta gaattcgcct actgtttctt agctgaattc tggttttgcc
    36121 tgccgtacag taacaatcca tttcttattc aaaatcatca taccatcgaa tttttattta
    36181 tgcaagcctt ttcatataaa aaataaaaaa actggaacac aagtctatct agaaaacaac
    36241 caaatcagag atgcacctct ttgcgtttct ctttttttca gcaaattggt ttttccttat
    36301 cgattcatat cctttggctg tttttaatct gtaacgactt atgtcccagt atcctaaaat
    36361 gtttttgtct ctagattgaa agcagatctc atatcatctg tcgcatcttt tatcgatgta
    36421 acactttcca gttctccttg tacaactagt cgcgtgacgg cgtccatgtc gccacaagta
    36481 attagtgtga caatttcttt tccctcgact tcattcagta attgggtatc tgtaggagca
    36541 actttttctt taaaaaatgt tttgtatgta tacacatttt ctaaatctgt caaatagatc
    36601 ttggcaccca tctctaaatt ttctagcggt gtaaacagca attcagggtt cgttgcacga
    36661 tggcttgcta aggcataatt tccttttccc atttcttgat ctggagataa tgttcctgct
    36721 ccgtaaagta gtgacgtatt atccaagcct ttgaaaaccg gtaaattgat tgaaactgaa
    36781 gggactgcga tactagcaat cactggtaga tctgttcctc tcatttgcga cctcatgacc
    36841 ccttctgtcg tcattggttc taccgcatca aagtcaaaac tgacatcttt atcgttattc
    36901 tttttcagat cttcttttgt tacattcgct atagcatatt tatctcctgt ctctctgaca
    36961 aaataatcct tgatctgttc attgaaaatc aaagctagtc ctactaataa caagaggaac
    37021 aagaggatat tgactagcca tctttttgct tttccttttt ttttcatttt ctctccctcc
    37081 agactcattt tactgttttt cttttgctct ttccaacagc cgaatgacac atttctacaa
    37141 ctctgtgtat ttttttcgta cacctgcgct tttttctaca ggatatttca aattattttt
    37201 gatcaatttt tcttcaatga tctcatctag atccaggtga aggttgtctg ccatcatgta
    37261 tgaatagatc aatacatctg ccagttcttc tttgattcgt tctaattgtg tttcttttac
    37321 ttcttccggc gttttccatt gaaaaagttc taacaattca gatgcttcca aagaaataga
    37381 gatagccaaa tctttttcgt tatgaaattg tcgccaatta cgttcatctc taaactggtt
    37441 gactttttcc atactattca tttgcttgcc ttcttccaat cacttcttac ttgattaatc
    37501 ataccatttt tttacataaa aaatagatga tgcttttaaa aaaactcctg ttttctttta
    37561 tttaaaataa atgtcataga aaatcaataa tttaggtagc caccatgtgt atgcagtagc
    37621 gtgtcttctt cccttgaggc tttttgtttt tcgatacgtt cttcttccca aacagacaac
    37681 gctactagaa tctctcttaa tgattttcct tcttcggtga gcgagtactc tactttaggc
    37741 gggacttggt tataggcttt tcgatggata ctttcatctt gttctaactc tcttaactgc
    37801 tgggtcagca ttttttttgt gatcgtaggg atttgcttgc ttctccacgc ctaaaataag
    37861 cgatattgct cttcccttta tctggtgtaa gcaaagcagt tagcattttg acgatcgtgg
    37921 gttccgcttt tcacttctta tctacttagt atcatgaagt atactttcgt actaatacta
    37981 agtagcggaa gtgtcaacta ctttgtttaa ctacatagca aaagaaacag aagggtgaca
    38041 acggtcatca cgcttctgtt tcttttgaat atgctgtgtt gttgtctcta tctccgtgta
    38101 gactttcctc aaacttatcc tttgacagta agtcagtagg ctgatttgct ctttctagat
    38161 gccagataca tttctacaaa aaaatagtga ttctgttttg aaaattcgat ttttagttct
    38221 gcattttcgt aaacatccaa catttttttc gcactgtata gacctaaccc gccatttttt
    38281 ttagaggttt ttcctctttt gaccatctcc gatagcgtca aattcgttgg atttgccttg
    38341 ttcgctatac taaaacgtga tccctctttt tcttccagca gattgatcgt aatcggactc
    38401 tgatcggcta cacgatgatc gaatgcgtta gaaataaaaa tagataaaca gcgaataaaa
    38461 acaaccagat caatcgggat tgttttcact tcgacaggaa taactacttg gatcgggatt
    38521 ttctccgatt ctgctttttc tagaaatttt aaaaagacac ccctgacagc tggggataca
    38581 atatttgtta gctgttgtat ttgcggtttt tcaaaaatag ctgcagaatg atcttctaat
    38641 atttctaaaa attccaacgt ttctttttta ttttcctgaa gagtaatttt taatgaaaac
    38701 agtagacttt tataatcatg tttgaattcc tctagatgat ggtaatattc ttcatttatt
    38761 tgatacagtt gaagcgattc ctgatgttgt ctttctttta cttggaaact gatcaacata
    38821 atggaaaaaa acaaagaaaa aaaactgatg actatcgaca aaagagatag aagcagataa
    38881 ttagatgagc ggaagtgaac acttcccaat accaagtaaa tcacgcccca gcttaataaa
    38941 atacaaatac ctccaaaata gtgttcagtt ggataagtaa aaagcaagtt gagaaatctc
    39001 gtctgtttgt ctatactttt tactaataga tagaaaataa atgcgataag tagtgcaaaa
    39061 aaacttgctg ttccaagatg tctctttcct aaataatcca tcagatcaaa aaccagaaaa
    39121 aagctgagtc cagtgatagc aaaaaaaaga ttactgatca aacagctgat aaacaggttt
    39181 ttcgtagtaa aataaaaaag aaacatcaat aataagccaa ctaggtaagg aaagctcact
    39241 ttagggatag gaagtaaaag tgtcaccacc atcaaagcta tacaaccaaa gctgtttttt
    39301 actagcagtt gtttcgaaaa gaataaatag atacctaaat ctaaaatcaa agtaaaggtc
    39361 aaataaagtg tcactgttgc gaccatctta ttcctccatt gttttgtttc aaaatgtaca
    39421 catatttttt taataggagc ctgcatatga aaaagtattt tatcatagaa gataaccagt
    39481 ttcacagcga atggatcatc agagaattgg tcaatcatca tatcgagaat atcgtgcagc
    39541 tcacttctat ctctcaaatc aatcagttga tccagttggg cactattgat aatcacgatg
    39601 ttttctttat cgatatcgac ttgaagcaat cactcacagg tattgatgtc gccgaacaaa
    39661 tacgtaaaaa aaatgcacat agtaatattg tttttttcac cgcttatacg aattttgcag
    39721 tagagatcat caatcggaaa atccttccga ttggatatat cagaaaagac aggaatttat
    39781 ctgaacaaat tcgtcaagtg ctagaacaga tcaattggaa agaacagcag atcgtaaatg
    39841 tcgataaaat ctttattgat caaaaaggtg gaagtctcgt cctgtatcct cgggaaattt
    39901 tatatattga aagtatcaaa ggtttgaaaa accaagttat ggtcaaaaca actacagaag
    39961 ataaattagt gaatacttct ttaaaacaat tgaagacaga gttagaaaag tatgcttact
    40021 ttttactttt aaaatcttac tgtatcaact tatcgcaatt gcagaaaatt gattctgctc
    40081 attatgtgtt agaatttttc aacggtgact ccttactcgt tggtcgaaag atctttgaaa
    40141 agacaaagga acgtttccat gacttccaaa aaactgcttc gagttgatga ataagagctg
    40201 agacgtaagc atagcagatt tcagggagtg ggccttgaat cggctccact ccctgaaatc
    40261 gttgatatga ttttacgctt cctcttctgt aggagcatct gttaacgacc atgtgaatgt
    40321 tgaaacgtag ttgtctacag cataattgga tacaggaaca aataattcca ctgattgtct
    40381 cccttgttcc acattctctc ctaatgaata aatccatgtg ccaatccctt catttgctcc
    40441 tgcagttact agatctactg cctgatctgc tgggatttcg attccttcag cgccagttcc
    40501 agaaacggta gatggcattc cactgctatt agttgcgcta tttcgaatca cggcgttttt
    40561 gaatttcacc tttgaaccag ttagtttatt cgtatcagat gttgtaccaa taaattctgt
    40621 tcgtttgact gataatttcc aaccggcata atttccgctt ttatccgtta cttgtacgaa
    40681 attctgtact tgtgtttgtg ctccagtact gtccgttccg ctttgtaatg cagcatatac
    40741 tgtttttgct gtacctaact tgattgttcc aaaatcaaga tctggagctt ggtcaatcgt
    40801 cagtaatccg gatgttccct gatttggttc gtcaggtgta aaaggagtat caggatcatt
    40861 tggatcaaca ggaggaactg gaacaacgtc ttcaggattt gcagcatctg gtttggtcac
    40921 agaaatcgtt ccttctgtag tggctgagct cttattctca gcaaaaacac tttgtgtaca
    40981 taataataga cttccagcta gaacggtact taatgcaatt gtttttttca tttctttttc
    41041 cttctttctt ttaaaataaa taatccagca aataagccaa taagaatgac tccaacaagt
    41101 atcagccatc gccagtcaaa tgtttctggc tgatttttta aatcaatatc cttttcattc
    41161 aattggtccg ctgcatcatc agttatttca aaatcggtat ggagattcca gcgtttttct
    41221 ttccaagcac ccttcacaac cgcttgataa tgtcccggag ctaatttttt tccttctaat
    41281 gggacaagta aatcaaaatt tgtattgggt gctacatcta ttttttcttt ttttattgaa
    41341 aggtaaggct cttttgtttc ttttttgtag atttcaaccg atgtagtcaa gccgaacata
    41401 taagaagcat ttggattttg aagattcgcc ttaatcacat ttcttccatc gctttgatcc
    41461 gcataagctt tttttaattt caaatctggg gaagactctt tttcgctatt tcaaatcaag
    41521 attgctttca cttgagaaaa ctgattgacg acaccaactg tacttttgtt agaaacgttc
    41581 tctttttttt cttgaaacgt gatccctcct gcgaccacac cagccagctc tttttctggc
    41641 gctttcagtg aaagtgtctc agtgaccgca ctttgaggag gaacagttat ttctcccact
    41701 gtttttgcta agtttttcat atcataactt aatgtttcat caaatttact ttttgattct
    41761 ccatattcga tcacaccgtt tgaattcgtc gtagcggagt ggatcaatgt ctcaactgtt
    41821 actgatttat ctgttcggtt aaagaccgtg taggggattt cctgtgtttt cccaggctcc
    41881 atccttacat ttatatatgt tttgttctta tcaatctgat tagccggtaa ttcaaaatct
    41941 accgcaaaat tgaattcgga ggcatggaca ggtgtctgcc aaaggatgaa tccaaggaga
    42001 aggatcataa gtaaagagca gttgtttcga tgacttttat tccatattcg attcatcaat
    42061 catcctcctt gatctttatt ctgtcggcgc atctgctaat gtccatgtta tctctgctga
    42121 gtaattacca gctatctgat tttctttggt taagacaagt ttaaacgccc gaccgttatt
    42181 taaatattct gaataatctg cttgcacatt cgttgaattt tgtcctctga caatttccag
    42241 ttcttcttca ttcactaatt gatactcctc tcctttcttg aaatatagga catcctgcat
    42301 agaaaatcct gcatcatttg tgaaaggctg agataatttt atcgttaaga tccacggatt
    42361 tttcgtttct cgctgatcat aaatccataa tttttcctgt atatccattt ggccaaatgc
    42421 ttcttgtgtc aaaccagttg ctttgatctg accaaaatcg agtttggaag gggcaagaaa
    42481 tgagagacca ccttctacta cgatcttcgt agcaggtgca aaaggcgggg catcatgata
    42541 aacaactggc gtatcacttg gattgtgatt cccaatttca tcgttggtac ttggcttgtt
    42601 ctgcacaccc ttagcagtag cttctcctgc ttggtcgttt aatacaattt ctaatacatc
    42661 tcctgcaaaa agttgatttt ctgggataga aagaacaaat tcccctgtat catcaatagt
    42721 tgtttctgac aaaacagctt gatttttttt caatgaaacc aaggctcctg cttcacctgt
    42781 acctgtgatc gtgcgagaag ctgcattgat caatgtggta cttaaattag caggttcagg
    42841 aggaatcaca tcgactgtcg ttttggcaga gtccagttct gtcacttctt ggccacctct
    42901 tactttttta gcagcaacaa cttgcacttg atcctccgtt tttaatctct catcattggg
    42961 tacttgaact tcgaacaagc cgccttgttc gccttctccc catgggttat atcctgcccc
    43021 ttcattttca tctgtttgtc cttgcgtgag agcagtaatt tctacagatg atttcgtcgt
    43081 atctgcgtaa gtaatacgta aagtcacttc tacttcgcct gtccaagcat ctcttggctt
    43141 tgcctcgaca ccttcgggaa caacgacatg cccatagatt tttttatctg cattcgttgg
    43201 cactcttaag gattcaatca tcgggagctg attattggca ctgattcgat tataattttt
    43261 cattccggca cctgtctgag caaattcatt tgttaaacct gcactagggc ttgatacgaa
    43321 attgcctaag tcggtcccat tgatcgataa gtctgtcata tcagaaaaat aatccgggtc
    43381 tttttcaaag ttatcgaatg ttgcgcctgt ttttcgccat aaagcgattt ctgaatgggc
    43441 gatctttaaa gaagaatctc catttccttg gaatacccag ccgcctatgc cattggtctc
    43501 gcttccaatc cgtttgtttt ggaaatcaaa ataggtagga gattctaagt ccacattcaa
    43561 cacatcccca ctgatgatgc cggtcttatc ttcccctgtt ttcccgacca tgactaaata
    43621 agttttttca cctgccgtaa aatttaaatc aatatctcca gttgtatcta cagttgctcc
    43681 ggaatctgcg cgtatatcga tatttgattt ttctccagtg actgtaaaat cagctgaacc
    43741 tggactttct agaaaaccag aagcgtcata aaattggatc gcttgatttc cttgattgag
    43801 tctgttcacg taagatttgc catcacctgt atttcttacc ctcaatgagc ctccattggt
    43861 tactgtcagc ttttgcgtac cattctcaaa acgaactgcg ctggaacgcc cactctgctt
    43921 atcgatgtcg ataactgatc cggctccttc tacgatcaaa gaaaggcctt ttcctacaaa
    43981 acgcatccca ttgttcccgg gttgatcgtc atctccagcc attttgaccg agatttgtcc
    44041 accacttgat acatggattt gactagaatc tccttgcaag cgaaatccgc ccgtagttgc
    44101 tgtattgatg gtgattttcc caccattcga tacagtgact ttcgatcctg aacctctcat
    44161 ttggatgatt ccgcgattgt tgtcattggt aatattgatc tttgtattta ccgaaaattc
    44221 gcttcccgaa ccactgactt caaacatgac attaccaggt agcgtatcga ctgcctctcc
    44281 ttcatcgcta acgccatctg cccgatcata atcaagagag acctttgaac cgcttaagat
    44341 atggattcct ggcgctgttt ttccactacc agtctcatct cctgaatcta gaaaaccgta
    44401 gaatttccct agatacggtc cataaagttg actaccattg atcacattca attgatagct
    44461 tctacaaaaa taggctttac ccggagcggc attcagtcct tccttcactg gttggacatc
    44521 caacacaccg gaaacaccat ctaatgtgac agttgcacga ggagcgtaaa tcagtcctaa
    44581 tttggaccca tcatacaaat caagtgtccc ggtaaatttt atttctcccg taaaatgata
    44641 tctgcttccc caaaaatcgg tcatataagc aatgataatg gcatttccat cgatatttgg
    44701 tgtcaatcca cctggatgtg cagctgttcc tttaaaattc tggaactcga tcgactgagg
    44761 tatctcattg atataaatat ggtgtgtgcc gaaattgtga gcgaatccac caaaatcaat
    44821 aacgatatct cgtttgatct ctaattccct gtctaaagtc aaaggctgaa tcagtcggat
    44881 cttagttact gattcgtctg cgagaagctg cgccagttct tctgttgtgg cagcttcgct
    44941 gtacccatcc gctgaaacag ctcttgccag tgccgcttca ctgctatcag acgtttgttc
    45001 agctacttct gaggtctcac ttgtcttatt ttcttcttgc cttttattag tagaagcctc
    45061 actttgagat tctgtagttt cagaagtaat cgattcttct gaaaaagtag tcttgtcaaa
    45121 actttgattc tctgattgcg ttgattcgtt tgttttctct acatcggcaa aaacggatgt
    45181 caccatcaag agagggaaga caaacaaaat cgacgcaatc agctttttta cacattgatt
    45241 cattctccac catcctctct gacgttttga caattttatt ttatcgtttt aatctatttc
    45301 caattgtact ttcatattta aaatatgtat ttttatggaa aaatttttct attaggtgta
    45361 aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc ttgtcacagt cccttatccc
    45421 gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc tccattttaa caataaacct
    45481 gtaaatgtga gggcaatact ttgattgtca caggtaattt atccccttca tctccatcaa
    45541 cattcgtcgt caattcttcc tcatcagcta atgacacaac taattgctca caagtaaggt
    45601 aggctagatt tttcgtagat tcatctactc cttttaatag gtctggcaat gttttaagcg
    45661 tatcgatcaa agaagaatct tttaaataaa ccaaatggag cttaccatca tccacttttg
    45721 catttggaag taatgtttca aaaccgccga ctgagttagt tagtcctatc agcaaggtac
    45781 tgctctcaat ttcttccttc tttccatcaa cttctacgtg aaacgagtag gattgtgtgg
    45841 aagctaactg tttgattcct gacatgaaat aagctagttt tccaaatttc gtcttcttct
    45901 ccgcatctac atcattgatt gattcaggga tagagccaat tgcgacgaca ttcataaaat
    45961 agtcgtcatt tatttttcca atatccaatg gcttgactga ttcaatcgaa aaatgattga
    46021 tcgcttcctc aggttctagc ggtatcccta atgctcgagc caagtcattc accgtaccca
    46081 atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc gattccactg attccttcgt
    46141 tgactgttcc atctccgccc atgacaaaca cactatgata cccttccgta gccgcttcgc
    46201 gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa gacgaccact tcatcaaaca
    46261 ccgattccaa ttttgctatc gctaattgct caaattcctt tgcttgttca ccgcctgaac
    46321 tggggtttac cactaataaa acttttttca tcactttctt tcccttctga ttgatttctt
    46381 taatataacg atagcataaa gaaaaaaatt aggctgtgat gaagtactgt tcatgaaaca
    46441 ttcatttatt ttttgacaag ataaaacatc catactatga aactgaggaa aattaaaaca
    46501 atgatatagg aaataaaaga aaatgatagg cattgtatac ctaataaagt aaaaatctta
    46561 ggtggcagat aaaaacacaa aaatagcaag agaatgtaat agcgtttctt ccactttttg
    46621 ttatcagacc ttatagcgat cagaggaata ccaaatgcat aactgaaaaa ctcttgatgt
    46681 actgttaata tgtcacggcc ttgaataata aatgcgacaa tccccgctat aattagtgtg
    46741 cctaacacca aaacggaaaa ttgatgaata tattttatat acaaatgatc cattgtactt
    46801 cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt gaataacttc tagaaataaa
    46861 agaaatttgc aaaaccactc catttatcta cttctacgtg cattttttga tattgttttc
    46921 tttatttaag attatacaga aaaatatcta gggtcgctgg attctaagta aaaaaaattt
    46981 ttaaaataaa aagaactaaa tcaagtagt
//
LOCUS       DAYYHB010000015        43607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000015 DAYYHB010000000
VERSION     DAYYHB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.015"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACHA6B_000750"
     CDS             160..840
                     /locus_tag="ACHA6B_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8948344.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACHA6B_000751"
     CDS             930..1964
                     /locus_tag="ACHA6B_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8948345.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACHA6B_000752"
     CDS             1978..2313
                     /locus_tag="ACHA6B_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948346.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACHA6B_000753"
     CDS             2297..4777
                     /locus_tag="ACHA6B_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948347.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACHA6B_000754"
     CDS             4833..6392
                     /locus_tag="ACHA6B_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948348.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACHA6B_000755"
     CDS             6389..7753
                     /locus_tag="ACHA6B_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8948349.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACHA6B_000756"
     CDS             7924..8994
                     /locus_tag="ACHA6B_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948350.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACHA6B_000757"
     CDS             9009..9677
                     /locus_tag="ACHA6B_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948351.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACHA6B_000758"
     CDS             9697..10275
                     /locus_tag="ACHA6B_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948352.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACHA6B_000759"
     CDS             10676..11587
                     /locus_tag="ACHA6B_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8948353.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACHA6B_000760"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACHA6B_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8948354.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACHA6B_000761"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACHA6B_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8948355.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACHA6B_000762"
     CDS             13579..14127
                     /locus_tag="ACHA6B_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8948356.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACHA6B_000763"
     CDS             14242..16602
                     /locus_tag="ACHA6B_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8948357.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACHA6B_000764"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACHA6B_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8948358.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACHA6B_000765"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACHA6B_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8948359.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACHA6B_000766"
     CDS             19108..20007
                     /locus_tag="ACHA6B_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948360.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACHA6B_000767"
     CDS             20000..21262
                     /locus_tag="ACHA6B_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948361.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACHA6B_000768"
     CDS             21423..23321
                     /locus_tag="ACHA6B_000768"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8948362.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACHA6B_000769"
     CDS             complement(23651..24370)
                     /locus_tag="ACHA6B_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8948363.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACHA6B_000770"
     CDS             24707..25039
                     /locus_tag="ACHA6B_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948364.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACHA6B_000771"
     CDS             25026..27029
                     /locus_tag="ACHA6B_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8948365.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACHA6B_000772"
     CDS             27048..27518
                     /locus_tag="ACHA6B_000772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8948366.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACHA6B_000773"
     CDS             27539..28123
                     /locus_tag="ACHA6B_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8948367.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACHA6B_000774"
     CDS             28138..29124
                     /locus_tag="ACHA6B_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8948368.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACHA6B_000775"
     CDS             29114..29425
                     /locus_tag="ACHA6B_000775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8948369.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACHA6B_000776"
     CDS             29447..31228
                     /locus_tag="ACHA6B_000776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8948370.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACHA6B_000777"
     CDS             31221..32597
                     /locus_tag="ACHA6B_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8948371.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACHA6B_000778"
     CDS             32604..33239
                     /locus_tag="ACHA6B_000778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8948372.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACHA6B_000779"
     CDS             33269..34624
                     /locus_tag="ACHA6B_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8948373.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACHA6B_000780"
     CDS             complement(34816..36210)
                     /locus_tag="ACHA6B_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8948374.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACHA6B_000781"
     CDS             36302..37273
                     /locus_tag="ACHA6B_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8948375.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACHA6B_000782"
     CDS             37270..38052
                     /locus_tag="ACHA6B_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8948376.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACHA6B_000783"
     CDS             complement(38221..38592)
                     /locus_tag="ACHA6B_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8948377.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACHA6B_000784"
     CDS             38726..39169
                     /locus_tag="ACHA6B_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8948378.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACHA6B_000785"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACHA6B_000785"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8948379.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACHA6B_000786"
     CDS             complement(40040..41407)
                     /locus_tag="ACHA6B_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8948380.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACHA6B_000787"
     CDS             complement(41391..42221)
                     /locus_tag="ACHA6B_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8948381.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACHA6B_000788"
     CDS             42384..42821
                     /locus_tag="ACHA6B_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8948382.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACHA6B_000789"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACHA6B_000789"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8948383.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14280 a         7493 c         9252 g        12582 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt ctttttt
//
LOCUS       DAYYHB010000016        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000016 DAYYHB010000000
VERSION     DAYYHB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.016"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA6B_000790"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA6B_000790"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8948384.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA6B_000791"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA6B_000791"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8948385.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA6B_000792"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA6B_000792"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8948386.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHA6B_000793"
     CDS             2179..2499
                     /locus_tag="ACHA6B_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8948387.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="ACHA6B_000794"
     CDS             complement(2611..3480)
                     /locus_tag="ACHA6B_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8948388.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHA6B_000795"
     CDS             complement(3576..4880)
                     /locus_tag="ACHA6B_000795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8948389.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHA6B_000796"
     CDS             complement(4877..5386)
                     /locus_tag="ACHA6B_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948390.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHA6B_000797"
     CDS             complement(5561..7696)
                     /locus_tag="ACHA6B_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8948391.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHA6B_000798"
     CDS             complement(7779..9434)
                     /locus_tag="ACHA6B_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8948392.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHA6B_000799"
     CDS             complement(9436..9750)
                     /locus_tag="ACHA6B_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8948393.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHA6B_000800"
     CDS             9920..10180
                     /locus_tag="ACHA6B_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8948394.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHA6B_000801"
     CDS             10194..10508
                     /locus_tag="ACHA6B_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8948395.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHA6B_000802"
     CDS             complement(11238..11792)
                     /locus_tag="ACHA6B_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8948396.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHA6B_000803"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHA6B_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHA6B_000804"
     CDS             complement(14153..14869)
                     /locus_tag="ACHA6B_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8948397.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA6B_000805"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA6B_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8948398.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA6B_000806"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA6B_000806"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8948399.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHA6B_000807"
     CDS             complement(18339..19799)
                     /locus_tag="ACHA6B_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948400.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHA6B_000808"
     CDS             complement(19789..21084)
                     /locus_tag="ACHA6B_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8948401.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHA6B_000809"
     CDS             complement(21416..22093)
                     /locus_tag="ACHA6B_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8948402.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHA6B_000810"
     CDS             complement(22123..23214)
                     /locus_tag="ACHA6B_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8948403.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHA6B_000811"
     CDS             complement(23315..23749)
                     /locus_tag="ACHA6B_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8948404.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHA6B_000812"
     CDS             complement(24333..25790)
                     /locus_tag="ACHA6B_000812"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8948405.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHA6B_000813"
     CDS             complement(25787..27682)
                     /locus_tag="ACHA6B_000813"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8948406.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHA6B_000814"
     CDS             complement(27740..29515)
                     /locus_tag="ACHA6B_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8948407.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHA6B_000815"
     CDS             complement(29565..31061)
                     /locus_tag="ACHA6B_000815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8948408.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHA6B_000816"
     CDS             complement(31255..32877)
                     /locus_tag="ACHA6B_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8948409.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHA6B_000817"
     CDS             complement(33205..34518)
                     /locus_tag="ACHA6B_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8948410.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHA6B_000818"
     CDS             complement(34763..34987)
                     /locus_tag="ACHA6B_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948411.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHA6B_000819"
     CDS             complement(35007..35429)
                     /locus_tag="ACHA6B_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8948412.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHA6B_000820"
     CDS             complement(35571..36281)
                     /locus_tag="ACHA6B_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8948413.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHA6B_000821"
     CDS             complement(36299..36796)
                     /locus_tag="ACHA6B_000821"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8948414.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHA6B_000822"
     CDS             complement(36833..37285)
                     /locus_tag="ACHA6B_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8948415.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHA6B_000823"
     CDS             complement(37420..38025)
                     /locus_tag="ACHA6B_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8948416.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHA6B_000824"
     CDS             complement(38475..39095)
                     /locus_tag="ACHA6B_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8948417.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHA6B_000825"
     CDS             complement(39097..39966)
                     /locus_tag="ACHA6B_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8948418.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHA6B_000826"
     CDS             complement(39989..40396)
                     /locus_tag="ACHA6B_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8948419.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHA6B_000827"
     CDS             complement(40415..41704)
                     /locus_tag="ACHA6B_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8948420.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAYYHB010000017        39883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000017 DAYYHB010000000
VERSION     DAYYHB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.017"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..921)
                     /locus_tag="ACHA6B_000828"
     CDS             complement(73..921)
                     /locus_tag="ACHA6B_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8948421.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA6B_000829"
     CDS             complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA6B_000829"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8948422.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3422..3601)
                     /locus_tag="ACHA6B_000830"
     CDS             complement(3422..3601)
                     /locus_tag="ACHA6B_000830"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8948423.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3648..5471)
                     /locus_tag="ACHA6B_000831"
     CDS             complement(3648..5471)
                     /locus_tag="ACHA6B_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8948424.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5749..8028
                     /gene="helD"
                     /locus_tag="ACHA6B_000832"
     CDS             5749..8028
                     /gene="helD"
                     /locus_tag="ACHA6B_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8948425.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8121..8768)
                     /locus_tag="ACHA6B_000833"
     CDS             complement(8121..8768)
                     /locus_tag="ACHA6B_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948426.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8947..9666)
                     /locus_tag="ACHA6B_000834"
     CDS             complement(8947..9666)
                     /locus_tag="ACHA6B_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8948427.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9797..10948)
                     /locus_tag="ACHA6B_000835"
     CDS             complement(9797..10948)
                     /locus_tag="ACHA6B_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8948428.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11165..11905)
                     /locus_tag="ACHA6B_000836"
     CDS             complement(11165..11905)
                     /locus_tag="ACHA6B_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8948429.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA6B_000837"
     CDS             complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA6B_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8948430.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA6B_000838"
     CDS             complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA6B_000838"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8948431.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12866..13516)
                     /locus_tag="ACHA6B_000839"
     CDS             complement(12866..13516)
                     /locus_tag="ACHA6B_000839"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8948432.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA6B_000840"
     CDS             complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA6B_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8948433.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA6B_000841"
     CDS             complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA6B_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8948434.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14959..15486)
                     /locus_tag="ACHA6B_000842"
     CDS             complement(14959..15486)
                     /locus_tag="ACHA6B_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8948435.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15666..16451)
                     /locus_tag="ACHA6B_000843"
     CDS             complement(15666..16451)
                     /locus_tag="ACHA6B_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8948436.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA6B_000844"
     CDS             complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA6B_000844"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8948437.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17314..18693)
                     /locus_tag="ACHA6B_000845"
     CDS             complement(17314..18693)
                     /locus_tag="ACHA6B_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8948438.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA6B_000846"
     CDS             complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA6B_000846"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8948439.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA6B_000847"
     CDS             complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA6B_000847"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8948440.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA6B_000848"
     CDS             complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA6B_000848"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8948441.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGTKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA6B_000849"
     CDS             complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA6B_000849"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8948442.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA6B_000850"
     CDS             complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA6B_000850"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8948443.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22651..22875)
                     /locus_tag="ACHA6B_000851"
     CDS             complement(22651..22875)
                     /locus_tag="ACHA6B_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8948444.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22928..23890)
                     /locus_tag="ACHA6B_000852"
     CDS             complement(22928..23890)
                     /locus_tag="ACHA6B_000852"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8948445.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23900..24340)
                     /locus_tag="ACHA6B_000853"
     CDS             complement(23900..24340)
                     /locus_tag="ACHA6B_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8948446.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24646..25611
                     /locus_tag="ACHA6B_000854"
     CDS             24646..25611
                     /locus_tag="ACHA6B_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8948447.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25668..25898)
                     /locus_tag="ACHA6B_000855"
     CDS             complement(25668..25898)
                     /locus_tag="ACHA6B_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8948448.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26020..26871)
                     /locus_tag="ACHA6B_000856"
     CDS             complement(26020..26871)
                     /locus_tag="ACHA6B_000856"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8948449.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKL"
     gene            complement(26960..28183)
                     /locus_tag="ACHA6B_000857"
     CDS             complement(26960..28183)
                     /locus_tag="ACHA6B_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8948450.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDIIQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28200..29246)
                     /locus_tag="ACHA6B_000858"
     CDS             complement(28200..29246)
                     /locus_tag="ACHA6B_000858"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8948451.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA6B_000859"
     CDS             complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA6B_000859"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8948452.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31137..31403)
                     /locus_tag="ACHA6B_000860"
     CDS             complement(31137..31403)
                     /locus_tag="ACHA6B_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8948453.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31882..34116
                     /locus_tag="ACHA6B_000861"
     CDS             31882..34116
                     /locus_tag="ACHA6B_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8948454.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34264..34584
                     /locus_tag="ACHA6B_000862"
     CDS             34264..34584
                     /locus_tag="ACHA6B_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8948455.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34789..35013)
                     /locus_tag="ACHA6B_000863"
     CDS             complement(34789..35013)
                     /locus_tag="ACHA6B_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8948456.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35211..36791)
                     /locus_tag="ACHA6B_000864"
     CDS             complement(35211..36791)
                     /locus_tag="ACHA6B_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8948457.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37192..38568)
                     /locus_tag="ACHA6B_000865"
     CDS             complement(37192..38568)
                     /locus_tag="ACHA6B_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8948458.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38696..39784)
                     /locus_tag="ACHA6B_000866"
     CDS             complement(38696..39784)
                     /locus_tag="ACHA6B_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8948459.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11321 a         8573 c         6891 g        13098 t
ORIGIN      
        1 tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa
       61 atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt
      121 cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt
      181 agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct
      241 agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa
      301 cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact
      361 tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg
      421 gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg
      481 cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc
      541 cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc
      601 ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt
      661 tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat
      721 tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc
      781 actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt
      841 accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc
      901 aatacgacta attacgctca taagttcctc caccctttta atgatagtct atgtaagtat
      961 accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc
     1021 caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg
     1081 aatacctgtt atctattgtt cggattgctt tctatctact acttttttac aggaaattta
     1141 cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat
     1201 tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt
     1261 ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta
     1321 tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca
     1381 aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg
     1441 ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag
     1501 cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat
     1561 tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt
     1621 gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg
     1681 gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag
     1741 cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg
     1801 tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat
     1861 atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa
     1921 caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac
     1981 ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat
     2041 aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa
     2101 caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc
     2161 agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt
     2221 aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc
     2281 cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa
     2341 gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt
     2401 ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt
     2461 ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc
     2521 cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag
     2581 taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa
     2641 aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga
     2701 cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc
     2761 cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat
     2821 ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc
     2881 caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac
     2941 gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt
     3001 ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac
     3061 catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag
     3121 atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa
     3181 aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc
     3241 cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa
     3301 aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca
     3361 tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa
     3421 cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga
     3481 catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca
     3541 tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca
     3601 taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt
     3661 gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa
     3721 atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc
     3781 gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc
     3841 gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct
     3901 ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca
     3961 ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca
     4021 acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta
     4081 cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca
     4141 tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt
     4201 gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt
     4261 gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt
     4321 ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc
     4381 agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag
     4441 atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt
     4501 ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta
     4561 tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg
     4621 atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc
     4681 agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac
     4741 caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca
     4801 gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt
     4861 tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt
     4921 aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc
     4981 aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt
     5041 tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc
     5101 aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt
     5161 tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc
     5221 aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc
     5281 gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc
     5341 aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga
     5401 aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg
     5461 aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc
     5521 cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct
     5581 taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa
     5641 tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact
     5701 atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa
     5761 caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg
     5821 ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct
     5881 aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc
     5941 caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg
     6001 caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac
     6061 cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca
     6121 atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc
     6181 gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt
     6241 aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac
     6301 tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg
     6361 atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa
     6421 ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat
     6481 cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt
     6541 tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca
     6601 ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa
     6661 gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc
     6721 ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt
     6781 gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa
     6841 gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg
     6901 aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg
     6961 gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag
     7021 gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc
     7081 catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta
     7141 caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa
     7201 catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta
     7261 ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat
     7321 atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg
     7381 ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa
     7441 acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta
     7501 acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa
     7561 gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca
     7621 acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc
     7681 cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct
     7741 caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg
     7801 aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt
     7861 ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac
     7921 accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct
     7981 ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa
     8041 ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta
     8101 attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt
     8161 aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca
     8221 aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat
     8281 tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc
     8341 tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc
     8401 ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt
     8461 agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga
     8521 cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc
     8581 atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct
     8641 tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct
     8701 aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt
     8761 gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta
     8821 tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga
     8881 caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact
     8941 ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc
     9001 cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt
     9061 ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc
     9121 ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc
     9181 cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc
     9241 ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac
     9301 acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac
     9361 attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg
     9421 caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat
     9481 atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc
     9541 atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata
     9601 aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg
     9661 tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc
     9721 cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc
     9781 atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat
     9841 tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt
     9901 tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag
     9961 catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct
    10021 ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt
    10081 ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca
    10141 tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag
    10201 ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg
    10261 aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg
    10321 catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc
    10381 ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct
    10441 ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat
    10501 acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt
    10561 cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt
    10621 aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca
    10681 agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa
    10741 gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga
    10801 tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg
    10861 ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt
    10921 actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa
    10981 gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta
    11041 ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc
    11101 gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa
    11161 accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt
    11221 ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat
    11281 aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag
    11341 acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc
    11401 ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg
    11461 gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt
    11521 ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc
    11581 acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca
    11641 tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca
    11701 tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa
    11761 ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat
    11821 gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa
    11881 tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg
    11941 gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat
    12001 gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac
    12061 gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga
    12121 tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc
    12181 gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt
    12241 ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc
    12301 tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa
    12361 atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat
    12421 ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata
    12481 aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg
    12541 aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc
    12601 atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac
    12661 cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact
    12721 agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc
    12781 aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc
    12841 tctagaataa gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa
    12901 tataatcaat tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt
    12961 gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg
    13021 gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct
    13081 catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat
    13141 aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc
    13201 caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt
    13261 ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct
    13321 gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca
    13381 tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt
    13441 tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag
    13501 cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat
    13561 acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt
    13621 cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt
    13681 gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca
    13741 ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc
    13801 tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca
    13861 atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg
    13921 ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa
    13981 actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata
    14041 atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat
    14101 ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc
    14161 ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga
    14221 tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat
    14281 ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga
    14341 ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg
    14401 tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat
    14461 agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag
    14521 atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt
    14581 ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt
    14641 tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac
    14701 cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat
    14761 tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc
    14821 gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg
    14881 tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga
    14941 tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc
    15001 taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc
    15061 tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat
    15121 atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt
    15181 ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc
    15241 aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg
    15301 gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt
    15361 attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt
    15421 taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga
    15481 aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc
    15541 gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa
    15601 gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa
    15661 aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat
    15721 cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat
    15781 cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg
    15841 cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct
    15901 ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc
    15961 atccagactt catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg
    16021 atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc
    16081 tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag
    16141 gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag
    16201 ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt
    16261 tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct
    16321 tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca
    16381 aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct
    16441 gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt
    16501 ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc
    16561 agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc
    16621 agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact
    16681 ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt
    16741 gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc
    16801 ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc
    16861 tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa
    16921 attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag
    16981 gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg
    17041 aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc
    17101 aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca
    17161 tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc
    17221 ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat
    17281 ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg
    17341 tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac
    17401 tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat
    17461 aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac
    17521 gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc
    17581 actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg
    17641 agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc
    17701 ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg
    17761 tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg
    17821 agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc
    17881 ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct
    17941 ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg
    18001 taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg
    18061 aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa
    18121 tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg
    18181 aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc
    18241 aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg
    18301 aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc
    18361 atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc
    18421 aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac
    18481 gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc
    18541 aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata
    18601 tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc
    18661 accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta
    18721 attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg
    18781 aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct
    18841 tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa
    18901 gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca
    18961 taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct
    19021 tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat
    19081 aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct
    19141 ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt
    19201 ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca
    19261 tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag
    19321 ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga
    19381 ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt
    19441 cagatatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca
    19501 tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga
    19561 attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca
    19621 aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga
    19681 attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc
    19741 aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc
    19801 cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga
    19861 tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt
    19921 tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg
    19981 ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc
    20041 atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc
    20101 tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc
    20161 tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt
    20221 aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca
    20281 aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg
    20341 gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat
    20401 accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg
    20461 tacgaacatt ggagaaactc gttttgtacc tttttcatgt agacgaataa cttggttttc
    20521 gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat
    20581 tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat
    20641 cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc
    20701 tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc
    20761 gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg
    20821 tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta
    20881 tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg
    20941 tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat
    21001 ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat
    21061 gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga
    21121 ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc
    21181 aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt
    21241 tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt
    21301 gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac
    21361 taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc
    21421 ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc
    21481 tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc
    21541 aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt
    21601 tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc
    21661 ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat
    21721 acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata
    21781 ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct
    21841 ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga
    21901 atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa
    21961 ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca
    22021 gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt
    22081 gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc
    22141 ggcgcattca tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa
    22201 gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact
    22261 aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc
    22321 ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc
    22381 tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca
    22441 cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct
    22501 tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata
    22561 tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa
    22621 atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac
    22681 taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc
    22741 aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc
    22801 agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat
    22861 tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat ttttataatt
    22921 taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa
    22981 atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata
    23041 agatagggat gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt
    23101 cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca
    23161 aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc
    23221 gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat
    23281 cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg
    23341 taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa
    23401 tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct
    23461 tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt
    23521 cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag
    23581 catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg
    23641 gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta
    23701 tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc
    23761 gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa
    23821 gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa
    23881 attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg
    23941 catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt
    24001 cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact
    24061 ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact
    24121 tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag
    24181 ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat
    24241 ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac
    24301 taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta
    24361 gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc
    24421 ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac
    24481 tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca catattttga
    24541 ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc
    24601 aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa
    24661 atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc
    24721 atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat
    24781 aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc
    24841 gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt
    24901 cctttttata accgacaaat gaattatttc agtaattata attatgtaca tattaccaat
    24961 aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat
    25021 aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct
    25081 gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa
    25141 cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac
    25201 ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt
    25261 caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt
    25321 ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag
    25381 cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct
    25441 cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa
    25501 gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag
    25561 tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc
    25621 ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag
    25681 tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct
    25741 gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg
    25801 ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc
    25861 agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt
    25921 tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt
    25981 tttttatgat tttttttctg gtatttttcc ttcaggccat caaagtttga tcaaagcttg
    26041 ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt
    26101 agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt
    26161 gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata
    26221 attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa
    26281 atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc
    26341 aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt
    26401 aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc
    26461 accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc
    26521 atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg
    26581 tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga
    26641 ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt
    26701 actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg
    26761 atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg
    26821 gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac
    26881 ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt
    26941 cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa
    27001 tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat
    27061 aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta
    27121 aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt
    27181 cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag
    27241 catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt
    27301 gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat
    27361 cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca
    27421 attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat
    27481 aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa
    27541 tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt
    27601 tgctgatatc ctgctgaatg atatcagaac gtttgaattt ttcaatgtcg ataccagtag
    27661 gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac
    27721 ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg
    27781 attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt
    27841 aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc
    27901 cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat
    27961 accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca
    28021 ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga
    28081 cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc
    28141 tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac
    28201 tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt
    28261 tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga
    28321 taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata
    28381 aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac
    28441 actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc
    28501 ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct
    28561 atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca
    28621 attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg
    28681 tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac
    28741 aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt
    28801 tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga
    28861 atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc
    28921 agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg
    28981 ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact
    29041 ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg
    29101 atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc
    29161 agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca
    29221 ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata gtataacata
    29281 gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg
    29341 ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg
    29401 atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca
    29461 ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa
    29521 actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac
    29581 tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg cccattttcc
    29641 ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg
    29701 ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa
    29761 atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc
    29821 tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc
    29881 acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag
    29941 tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc
    30001 acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg
    30061 atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc
    30121 accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt
    30181 gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc
    30241 agtacggtac agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg
    30301 tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt
    30361 ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc
    30421 agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa
    30481 aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc
    30541 taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc
    30601 ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac
    30661 gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa
    30721 aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc
    30781 cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt
    30841 tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac
    30901 catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc
    30961 acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc
    31021 agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta
    31081 agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat
    31141 tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca
    31201 gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc
    31261 atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat
    31321 ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact
    31381 acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg
    31441 tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta
    31501 aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat
    31561 tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat
    31621 ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct
    31681 ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca
    31741 aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac
    31801 taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt
    31861 gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt
    31921 cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat
    31981 caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt
    32041 ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga
    32101 aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag
    32161 ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa
    32221 gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt
    32281 gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt
    32341 aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa
    32401 ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata
    32461 cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat
    32521 gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga
    32581 aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt
    32641 ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt
    32701 atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt
    32761 ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca
    32821 gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat
    32881 ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca
    32941 atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat
    33001 ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt
    33061 cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa
    33121 caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac
    33181 ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg
    33241 aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc
    33301 ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa
    33361 ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat
    33421 agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa
    33481 ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa
    33541 gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat
    33601 gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga
    33661 actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct
    33721 gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc
    33781 atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac
    33841 gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa
    33901 gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg
    33961 atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat
    34021 gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt
    34081 ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg
    34141 tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt
    34201 tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc
    34261 gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa
    34321 aacactgatg cccatctagt caaggtctac acagccggaa ataccactat tgtctacact
    34381 gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa
    34441 gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag
    34501 gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa
    34561 ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac
    34621 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc
    34681 aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc
    34741 agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga
    34801 tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt
    34861 tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt
    34921 gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt
    34981 ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat
    35041 atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt ttaatatagt
    35101 aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt
    35161 gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa
    35221 ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg
    35281 aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc
    35341 ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg
    35401 attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga
    35461 ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct
    35521 tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca
    35581 gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt
    35641 tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata
    35701 tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg
    35761 cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt
    35821 ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg
    35881 atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt
    35941 gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca
    36001 ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt
    36061 tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca
    36121 tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct
    36181 actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt atggtagata
    36241 tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg
    36301 atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct
    36361 agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc
    36421 tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc
    36481 atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt
    36541 actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt
    36601 ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc
    36661 ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca
    36721 gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt
    36781 ggattattca taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac
    36841 agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta
    36901 gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc
    36961 caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata
    37021 gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa
    37081 tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag
    37141 acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct
    37201 tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc
    37261 tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc
    37321 ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat
    37381 catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc
    37441 actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg
    37501 ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac
    37561 gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa
    37621 taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat caataaagac
    37681 cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc
    37741 gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt
    37801 gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc
    37861 ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg
    37921 ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa
    37981 tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc
    38041 atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac
    38101 gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc
    38161 ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata
    38221 ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa
    38281 taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac
    38341 caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa
    38401 aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat
    38461 cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa
    38521 taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc
    38581 tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta
    38641 aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg
    38701 aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta
    38761 tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca
    38821 atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga tggattttca
    38881 gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg acgattagca
    38941 caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag
    39001 gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc
    39061 caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa
    39121 cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc
    39181 ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca
    39241 ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta
    39301 cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga
    39361 attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta
    39421 cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga
    39481 aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa
    39541 gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc
    39601 ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc
    39661 ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa
    39721 agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa
    39781 acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta
    39841 gtatatatta tattcgttaa aaattctttt attctttttt tat
//
LOCUS       DAYYHB010000018        38516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000018 DAYYHB010000000
VERSION     DAYYHB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.018"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..620)
                     /locus_tag="ACHA6B_000867"
     CDS             complement(102..620)
                     /locus_tag="ACHA6B_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8948460.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(622..1239)
                     /locus_tag="ACHA6B_000868"
     CDS             complement(622..1239)
                     /locus_tag="ACHA6B_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8948461.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1236..2090)
                     /locus_tag="ACHA6B_000869"
     CDS             complement(1236..2090)
                     /locus_tag="ACHA6B_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948462.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2087..2998)
                     /locus_tag="ACHA6B_000870"
     CDS             complement(2087..2998)
                     /locus_tag="ACHA6B_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948463.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3039..4373)
                     /locus_tag="ACHA6B_000871"
     CDS             complement(3039..4373)
                     /locus_tag="ACHA6B_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8948464.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4581..5411
                     /locus_tag="ACHA6B_000872"
     CDS             4581..5411
                     /locus_tag="ACHA6B_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8948465.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5655..5904)
                     /locus_tag="ACHA6B_000873"
                     /pseudo
     CDS             complement(5655..5904)
                     /locus_tag="ACHA6B_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..6289)
                     /locus_tag="ACHA6B_000874"
     CDS             complement(6095..6289)
                     /locus_tag="ACHA6B_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948466.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6794..6979)
                     /locus_tag="ACHA6B_000875"
     CDS             complement(6794..6979)
                     /locus_tag="ACHA6B_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948467.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7829..8170)
                     /locus_tag="ACHA6B_000876"
     CDS             complement(7829..8170)
                     /locus_tag="ACHA6B_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8948468.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8170..8400)
                     /locus_tag="ACHA6B_000877"
     CDS             complement(8170..8400)
                     /locus_tag="ACHA6B_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8948469.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8687..9025
                     /locus_tag="ACHA6B_000878"
     CDS             8687..9025
                     /locus_tag="ACHA6B_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948470.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9230..9792)
                     /locus_tag="ACHA6B_000879"
                     /pseudo
     CDS             complement(9230..9792)
                     /locus_tag="ACHA6B_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10524..11105)
                     /locus_tag="ACHA6B_000880"
     CDS             complement(10524..11105)
                     /locus_tag="ACHA6B_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948471.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11288..11914)
                     /locus_tag="ACHA6B_000881"
     CDS             complement(11288..11914)
                     /locus_tag="ACHA6B_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8948472.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11936..13267)
                     /locus_tag="ACHA6B_000882"
     CDS             complement(11936..13267)
                     /locus_tag="ACHA6B_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8948473.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13422..13823)
                     /locus_tag="ACHA6B_000883"
     CDS             complement(13422..13823)
                     /locus_tag="ACHA6B_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8948474.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13909..14115)
                     /locus_tag="ACHA6B_000884"
     CDS             complement(13909..14115)
                     /locus_tag="ACHA6B_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948475.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14210..15712)
                     /locus_tag="ACHA6B_000885"
     CDS             complement(14210..15712)
                     /locus_tag="ACHA6B_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8948476.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACHA6B_000886"
     CDS             complement(15730..16185)
                     /gene="argR"
                     /locus_tag="ACHA6B_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8948477.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16283..16900)
                     /locus_tag="ACHA6B_000887"
     CDS             complement(16283..16900)
                     /locus_tag="ACHA6B_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8948478.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17121..18494
                     /locus_tag="ACHA6B_000888"
     CDS             17121..18494
                     /locus_tag="ACHA6B_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8948479.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18569..19681
                     /locus_tag="ACHA6B_000889"
     CDS             18569..19681
                     /locus_tag="ACHA6B_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8948480.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19753..21390)
                     /locus_tag="ACHA6B_000890"
     CDS             complement(19753..21390)
                     /locus_tag="ACHA6B_000890"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8948481.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21558..22166)
                     /locus_tag="ACHA6B_000891"
     CDS             complement(21558..22166)
                     /locus_tag="ACHA6B_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8948482.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(22550..23701)
                     /gene="queG"
                     /locus_tag="ACHA6B_000892"
     CDS             complement(22550..23701)
                     /gene="queG"
                     /locus_tag="ACHA6B_000892"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8948483.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(23720..24472)
                     /locus_tag="ACHA6B_000893"
     CDS             complement(23720..24472)
                     /locus_tag="ACHA6B_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948484.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(24613..25562)
                     /locus_tag="ACHA6B_000894"
                     /pseudo
     CDS             complement(24613..25562)
                     /locus_tag="ACHA6B_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(25563..26402)
                     /locus_tag="ACHA6B_000895"
     CDS             complement(25563..26402)
                     /locus_tag="ACHA6B_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8948485.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(26399..27145)
                     /locus_tag="ACHA6B_000896"
     CDS             complement(26399..27145)
                     /locus_tag="ACHA6B_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8948486.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            27383..28753
                     /locus_tag="ACHA6B_000897"
     CDS             27383..28753
                     /locus_tag="ACHA6B_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8948487.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(28808..29704)
                     /locus_tag="ACHA6B_000898"
     CDS             complement(28808..29704)
                     /locus_tag="ACHA6B_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8948488.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(29818..29988)
                     /locus_tag="ACHA6B_000899"
     CDS             complement(29818..29988)
                     /locus_tag="ACHA6B_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948489.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(29985..30662)
                     /locus_tag="ACHA6B_000900"
     CDS             complement(29985..30662)
                     /locus_tag="ACHA6B_000900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8948490.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(30753..31658)
                     /locus_tag="ACHA6B_000901"
     CDS             complement(30753..31658)
                     /locus_tag="ACHA6B_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8948491.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            31833..33257
                     /locus_tag="ACHA6B_000902"
     CDS             31833..33257
                     /locus_tag="ACHA6B_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8948492.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            33303..34334
                     /locus_tag="ACHA6B_000903"
     CDS             33303..34334
                     /locus_tag="ACHA6B_000903"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8948493.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            34341..35528
                     /locus_tag="ACHA6B_000904"
     CDS             34341..35528
                     /locus_tag="ACHA6B_000904"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8948494.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            35684..37066
                     /locus_tag="ACHA6B_000905"
     CDS             35684..37066
                     /locus_tag="ACHA6B_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8948495.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            37038..37196
                     /locus_tag="ACHA6B_000906"
     CDS             37038..37196
                     /locus_tag="ACHA6B_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948496.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            37193..37423
                     /locus_tag="ACHA6B_000907"
     CDS             37193..37423
                     /locus_tag="ACHA6B_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948497.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(37623..38291)
                     /locus_tag="ACHA6B_000908"
     CDS             complement(37623..38291)
                     /locus_tag="ACHA6B_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8948498.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        11195 a         7788 c         6898 g        12635 t
ORIGIN      
        1 agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa ataaagcaca
       61 aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac ggcagcgcat
      121 aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa
      181 agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa tattcctgca
      241 actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt atgtcctgaa
      301 ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt tttttcataa
      361 ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa atagatcaaa
      421 ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac taaaacagca
      481 gcaaggagca accaaataaa accaccatta gagatattac tgataaattt caaaaaattg
      541 atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact atcatcgatt
      601 ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt gcgtacgatg
      661 gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag caaaatagat
      721 tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc
      781 gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt ctttttgttc
      841 cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca aaaacaacgt
      901 agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct gataatcgat
      961 caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa gagagaagtt
     1021 ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt ttttgtaatg
     1081 ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag gcaagacgca
     1141 caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga tttctaagaa
     1201 ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc tcctgtcaaa
     1261 tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag
     1321 acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt ctgaaaaact
     1381 tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag aggacctaag
     1441 aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc
     1501 aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat ttcagcagct
     1561 tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat attgaacgca
     1621 ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc gatcttggag
     1681 aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt agaaagaacc
     1741 aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata agcaataaaa
     1801 gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt atttcttgca
     1861 tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac gattttttct
     1921 gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga agtaaacacc
     1981 atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac ataacttagt
     2041 attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
     2101 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
     2161 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
     2221 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
     2281 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
     2341 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
     2401 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
     2461 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
     2521 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
     2581 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
     2641 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
     2701 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
     2761 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
     2821 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
     2881 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
     2941 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
     3001 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
     3061 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
     3121 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
     3181 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
     3241 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
     3301 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
     3361 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
     3421 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
     3481 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
     3541 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
     3601 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
     3661 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
     3721 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
     3781 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
     3841 tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat gacttgtgta
     3901 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
     3961 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
     4021 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
     4081 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
     4141 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
     4201 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
     4261 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
     4321 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
     4381 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt tgttctgaaa
     4441 ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata ctttatataa
     4501 ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa tgtattaaaa
     4561 atagattagg aggaattttg atgtttagac accagaaaga attgcagttt gaagcaaaac
     4621 cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc ggccaatatg
     4681 gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga ggagaagaaa
     4741 aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg gaaatgttgg
     4801 cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa gcagcgatgc
     4861 aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac gcgatcgtag
     4921 atggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt gcctatatca
     4981 ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa tcacaaggaa
     5041 gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt
     5101 ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca gtgaaagaat
     5161 tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac aaagaacgaa
     5221 aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt gaaggttctt
     5281 gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa gaacctaaga
     5341 tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg acacctccat
     5401 tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg ctttgtgagc
     5461 aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt ctctagagct
     5521 ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt atgttcaagc
     5581 catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac tccttagccc
     5641 ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc atgtttttct
     5701 tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt cacatagtat
     5761 ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca atctcttgat
     5821 gatctcctga atattggctc gatattgatt ataaattatt tttcttctat actttggtgt
     5881 gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc cctttattgc
     5941 tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa
     6001 agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa
     6061 atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt ttataaatgg
     6121 aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct atgaaatctt
     6181 tatttattag atattcaaca gaaatcccga taacactagc caacaatata tttatcagaa
     6241 gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata gactctcctt
     6301 tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta aaggggcgca
     6361 gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg tttaataact
     6421 ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac ctccaatttt
     6481 taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac ttatatatcg
     6541 aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta cttgcatcta
     6601 aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat taatttttaa
     6661 actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt ctgtctcatc
     6721 cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg gaatagttga
     6781 gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat acaatgaaaa
     6841 acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac tcattactgt
     6901 atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg gttaggaatc
     6961 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
     7021 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaccttac attatactgg
     7081 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
     7141 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
     7201 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
     7261 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
     7321 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
     7381 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
     7441 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
     7501 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
     7561 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
     7621 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
     7681 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
     7741 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
     7801 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
     7861 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
     7921 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     7981 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     8041 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     8101 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     8161 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     8221 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     8281 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     8341 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     8401 tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt
     8461 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
     8521 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
     8581 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
     8641 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
     8701 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
     8761 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
     8821 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
     8881 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
     8941 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
     9001 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
     9061 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
     9121 gatgcagtat atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc
     9181 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
     9241 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
     9301 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
     9361 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
     9421 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     9481 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     9541 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     9601 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     9661 cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg
     9721 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     9781 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     9841 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     9901 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     9961 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
    10021 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
    10081 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
    10141 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    10201 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
    10261 tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa
    10321 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
    10381 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
    10441 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    10501 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
    10561 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
    10621 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
    10681 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
    10741 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
    10801 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
    10861 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
    10921 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
    10981 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
    11041 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
    11101 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
    11161 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
    11221 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
    11281 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
    11341 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
    11401 tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca
    11461 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
    11521 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
    11581 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
    11641 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
    11701 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
    11761 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
    11821 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
    11881 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
    11941 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
    12001 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
    12061 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
    12121 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
    12181 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
    12241 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
    12301 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
    12361 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
    12421 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
    12481 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
    12541 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
    12601 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
    12661 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
    12721 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
    12781 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
    12841 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
    12901 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
    12961 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
    13021 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
    13081 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
    13141 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
    13201 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
    13261 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
    13321 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
    13381 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
    13441 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
    13501 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
    13561 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
    13621 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
    13681 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
    13741 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
    13801 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
    13861 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
    13921 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
    13981 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
    14041 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
    14101 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
    14161 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
    14221 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
    14281 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
    14341 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
    14401 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
    14461 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
    14521 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
    14581 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
    14641 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
    14701 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
    14761 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
    14821 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
    14881 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
    14941 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
    15001 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
    15061 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
    15121 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
    15181 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
    15241 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
    15301 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
    15361 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
    15421 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
    15481 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
    15541 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
    15601 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
    15661 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
    15721 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
    15781 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
    15841 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
    15901 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
    15961 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
    16021 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
    16081 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
    16141 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
    16201 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
    16261 ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca
    16321 ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag
    16381 ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt
    16441 cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata
    16501 ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta
    16561 tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt
    16621 tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga
    16681 cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat
    16741 agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga
    16801 tccctctgat gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca
    16861 tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt
    16921 ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact
    16981 atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg
    17041 cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa
    17101 attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa
    17161 ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt
    17221 tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga
    17281 tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta
    17341 gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt
    17401 tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag
    17461 gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag
    17521 gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt
    17581 atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg
    17641 ttttgtttct ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa
    17701 gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa
    17761 catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg
    17821 gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta
    17881 ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat
    17941 tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt
    18001 ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt
    18061 ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt
    18121 ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga
    18181 ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca
    18241 ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct
    18301 tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc
    18361 agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag
    18421 gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg
    18481 ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa
    18541 atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat
    18601 cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga
    18661 tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa
    18721 acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact
    18781 tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg
    18841 acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc
    18901 agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt
    18961 gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt
    19021 agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac
    19081 tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga
    19141 tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc
    19201 tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag
    19261 tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg
    19321 aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag
    19381 cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat
    19441 aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc
    19501 atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc
    19561 tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca
    19621 ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata
    19681 gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa
    19741 aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc
    19801 tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg
    19861 tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa
    19921 atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct
    19981 ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt
    20041 caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc
    20101 cctaagagga tagacacgga aaggattagc aaatggttca atgactagct catctagctg
    20161 tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga
    20221 cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat
    20281 ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg
    20341 tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc
    20401 tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc
    20461 atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc
    20521 attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt
    20581 atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt
    20641 ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt
    20701 gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg
    20761 taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa
    20821 tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac
    20881 agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga
    20941 atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc
    21001 agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc
    21061 ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg
    21121 agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc
    21181 tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact
    21241 cccttttgct agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta
    21301 aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc
    21361 gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac
    21421 gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa
    21481 cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc
    21541 attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc
    21601 catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa
    21661 tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta
    21721 gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt
    21781 ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg
    21841 tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt
    21901 tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg
    21961 cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct
    22021 gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca
    22081 gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct
    22141 aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt
    22201 tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac atgacattta
    22261 atgacaaaat aacgaattat ttaatatttg taacatttaa acaaatattt ttttgataca
    22321 aaaccataaa agccttgttg tataagcaaa gtaatcgttt ttctatgttt ttttattttt
    22381 gaatgaaaaa cgcagattct gtaacattca ctgcaataat ccataaaaaa aagcaaaaaa
    22441 atttttgatt tttatcaaat tctcttaaaa aaatgtaaaa aatacaaaaa aaccagtaat
    22501 tgttacaaca attactggac aaaaactttg aataatgtta actatttttt tattttaact
    22561 ttctccgcaa taatttctgg gcatgttcca attcgtttat aacgacttct tctgtttctt
    22621 tagctaatat ttcatctaat acatcaagcc atttttccgg ttcttttgtt cctagacgtc
    22681 caatagccca agctgctgtt cctctgatga cagggcggac atctgttaaa cattctgaaa
    22741 tctcaggcaa tgccgttcga tcacctagat tagctaaagc aatcaatgcg tttctctgca
    22801 aaggtttctt cccacgccat gaaccagcta aatgcccaaa ttgctgtttg aagtctttat
    22861 tagacatcga aagcattggc tttaattttg gatatatttc gtctactttt ggttccatct
    22921 cttcatgaaa atgaaaatct tttccttgat tgtaagggca gactaactgg cagatatcac
    22981 atccataaat gacatttcgc atttttttac gatattcttc cggcatcatc ccttttgtct
    23041 gagtttgata agacaaacat cgctgggcat tcattcttcc gtcaccaagc aatgcttttg
    23101 tcggacaagc agtcacgcag cgcatacagt caccgcaacc aaattctcct ggtttatcag
    23161 gctcaaatac aatattagta acgatctcac caagatagac aaatgaaccg aactcttcag
    23221 tgatcaacaa tccgtttcgt ccaataaatc ctaaaccagc tcgctgtgct acagcgacat
    23281 cgatcagttc tccagtatct acttgcggag ctagacgcca ttcctcttct ttttgccatt
    23341 tttctgctct tgtttggatg aattcgatca atcggttcaa acggtctctg agtatatcat
    23401 ggtaatcgat cccccaagaa gcacgagcaa actgcccgcg tttttcatcc ctaggcactt
    23461 cttcatggat cctagtagga tacgcaagtg caatggaaat gatcgatttc ggctgatcaa
    23521 atgtcatttc tggataaaga cgctcatcga tatttggatg ttcaaagcca gaagtatggc
    23581 cagctgcttt ttgttcttcc aacgattctt ttaatgaatc aaaaggttcc gccgtggtga
    23641 acccgatttt gtcaattcct aatcgtttgc tttcttgtat gatttcttct tttaatgtca
    23701 tggtcttcct cgcttgctgt taacggctgt ataacccata atatagtttt tcttttttca
    23761 gctggacaaa ctggatctgc tgatcggcaa tcttcttttc ttccatccat ttttttcttg
    23821 cagcaaatac ttcccaataa tttgttgaaa ttaattttct tttgttatct aatgcattaa
    23881 aaaaattcaa tgaaactggt tttgtactta acagttcatg gagccttacc tcttcccatg
    23941 gcgaaaactc agacagatcg cgggaataca atacttcttc atagagattt ttttgctgtg
    24001 tgtacgtgaa tgtttgcggg attttcccag gcctagcaat ttctactaga cgattataaa
    24061 acatattcat taatttgctg ttggcaataa attgactttt ataataggcc gactggatct
    24121 ttttgattac tccttctgct ttgatgactg aaaataatgc aggccatgct ttgacggtta
    24181 tttcgtcgtt ttgtttaact aatacgccag gtgtgaagcc gactggagaa aggaacacaa
    24241 aataaccgat accggtattt tcatctaaag gctgaaattc atcaaacaac catttgccta
    24301 tgtacagctc ttcatctggt tctgttgtct cattttgatt tgctatgaat aatttagcta
    24361 attctgcttc aagaaattga aggtcttgat ccttgtcggt attttcctgc tggctgatca
    24421 atcgaaagac ttcttcttta atcgtatatt tatctttacg tgtaaaattc atagccacac
    24481 cttccttcct tactctaact ataacggaat ttcttcaaaa aaggtatctg tacgacgaaa
    24541 aacagcataa ttttttttca tatctcttat aaaaaatgca aggtctgctc ctcaagctag
    24601 accttgcact tttcagcttt cttttgtttg tgacaaacct tcatggatcg tttcaatatt
    24661 ccatttcatc atctgatagt aagaatctcc ttgttctcct tcttttgcta tggaatcagt
    24721 gaacagttta tcatagattt tcaaacccgt ttcttttgct acccgttcca tgcttcttga
    24781 gtctacactg gtttccacga ataaaaccgg tacttctttt gctcttatct gatcaatgat
    24841 cgctttcatt tgatcaggcg taccttgact ttctgtattg atctcccata tataagctgc
    24901 tggcaaatca tatgctcgtg aaaaatactt gaatgcccct tcacttgtta ctaataattt
    24961 tttgttctct tcgatagaag caaaactttc cttcgcttgt gtatctaatt cttttagttt
    25021 ttccacatat gcttctgcat ttttttatag agtgtcgcat tttctgcatc tttttcagag
    25081 aatatacgtg cgatttcctc cacatatttt attccgttag ataggtctag ccaagcatgg
    25141 ggatctgctt ttgtatgttc ttcaccgcta gttaaataaa gaggttctac atttttgcta
    25201 actgcaaaat aatcttttcc ttcttctttt ttagccgttt ccatcaagtt atcgaaccag
    25261 ctgttacctg tttcaagatt caaaccgttg tataaaataa catctgcatc gcttgccttt
    25321 ttgatgtctt ctggtagtac ttcatattca tgcggatctg ttccgactgg tacgatactg
    25381 tggatatcta tttgatctgt tccgacctct tttgccatgt ccgccaagat cgaattggta
    25441 gccacgactg acaacttttc tttacttcct tcatccgctt ggtcagaagc ctgctttccg
    25501 caaccggcta ataccaagaa actaactgcc aagattaaaa agagtgattt ttttgttttc
    25561 atctatctgc ttcctttctg aaataaaatt ccttttgttg gagaaaataa aaaagcaagg
    25621 ataaaaaatg cggtacatgc taacacgatc gtagctcctg atggccaatt gtaactataa
    25681 ctgaaaaaca caccgacaac tgcacttagc ataccgattc ctgcagctgt aatgatcatt
    25741 ttcagtaaat ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa catggcaatc
    25801 accaagattg ttcctactgt ctgtaaactg actacagcca ctaatgtcaa aaagaacatc
    25861 aaaagataat ggatcagcga tgtgttcagc ccataagctt ttgccattgt cggatcgaat
    25921 gatgtgatct tcaattcttt ataaaagaaa aagacaaata tcaagacgat tccactaacg
    25981 agtgctgtca gcaacaaatc gctttcccta acagctaaga cattcccaaa taaaatatga
    26041 tacaaatctg tcgcactctg ggcatatgaa atcaagataa tccctaatgc aaaaaaggag
    26101 ctaaagacaa ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt cacgaaacca
    26161 atcactgctg ctgttagcat cccaaaagca gtggctccaa agatgtaact aaaaccaaac
    26221 atataagaaa tggctacacc tggaagaact gcgtgagaga tagcgtctcc catcaaagac
    26281 atccctcgta aaataataaa agaaccgatg actccagaag caagtcctac aagcatggaa
    26341 gtgaccaaag cgtattgcag gaattgataa ttcattaatc catcaataaa ggattggatc
    26401 attttttctc tcctcctttt actaaatgtc tgatgatctc accataggcc aattgaaggt
    26461 tcttcgatgt aaagacttct tgtactggac cggaagcgat cagctgttta tttaaaagaa
    26521 taacctcatc gaaatattct tctactttat gaagatcatg atggacgatc aaaatcgttt
    26581 ttcctgaatt cttcaattcc tgcaagatgt caaagatctt tcgttcactt aacgcatcaa
    26641 tccctacgaa tggttcatct aaaaagatcc attctgctcc ttgggctaga gctcttgcaa
    26701 taaaaactct ctgaagctgt ccacccgata gttcgctgat ctgtcttttg gcatattctt
    26761 ccatccctac ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc ccaggtcttt
    26821 gtccaattcg taaagatgga tatgttccta aaagtacaac gcctaataca tcaattggaa
    26881 aggaaagatc caattcactt cgctgttcca cgtaggctat ttttttacgg actgagttta
    26941 ccgactgtcc agaaaaggtg actttaccag tagtagctgg tatcaactct agcaaagatt
    27001 tcataaatgt tgattttcct gcaccgtttg gtccgataat accggtaatt ttttgtggat
    27061 ttatcgtcaa gttgatagtt ttcaaaacag ttttcccttg ataagcggct gaaaggtctt
    27121 gaattacaat ggccgtacgt tccaaatctt acactccttt ttaggtttac ctaacttttt
    27181 atttaaatat agtttaacta aaatggaaag cattttcaag tagaatgatg aaaaagtttt
    27241 tttcgaattt tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa gagttcaata
    27301 aggctaacct aataatgaaa aacatgtgtt gaaacgtttg attatttttt cttgttgact
    27361 caacaacgaa aggagttttt gtatggaaga agaaaagtta actcgaagtc ttagtgcccg
    27421 tcatatccaa atgatcgcac taggtggaac gatcggtgtc ggtctgttta tgggtgcctc
    27481 ctcaaccata cgttggacag ggccctcagt gatgggagcc tatgcgattg caggattctt
    27541 tttgtatttg attatgcgtg ctttaggcga aatgctttac gttgatcctt cgactggttc
    27601 ttttgcaaat tacgcgagcg agtacatcca tcctgtggct ggctacttga ccgcttggag
    27661 taatattttt caatatattg tcgtaggaat aagtgaagtg attgctgtag gatcttatat
    27721 gaattattgg tggcctgatt tgccggcaat tattcccgga atcattgtcg tcttgtttct
    27781 tatgctggca aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt ggttttcgat
    27841 catcaaagtc ataacgatca ttttgatgat cattgcaggt cttggggtaa tcttatttgg
    27901 atttggtaat catggtcagg cagtagggat ttcaaatctt tggaaaaatg gtggattctt
    27961 cactggaggc gtcaaaggat ttttctttgc attatcgatc gttgttgctt cctatcaagg
    28021 aatcgaactg atcggaatga ctgctggtga agcagagaat cctaaaaaga cgatcattga
    28081 agctgtccag tccactatcg gtcgaatctt gattttttat attggtgcta tttttatcat
    28141 tgtgagtatc tatccgtgga atcaattgag tgagattggt tcaccttttg tacaaacttt
    28201 ttctaaagtc ggcattacat ttgctgcagg actgattaat tttgtcgtga ttacagcggc
    28261 attatcaggt tgcaattcag ggattttcag cgcaagtcgt atggtctata cacttgccat
    28321 aaatggaaag atgtctaaaa aatttctgaa actatctcgt cacggtgcgc ctttttatcc
    28381 tgtcgtagca atctcaggtg gaatcttaat tggtctgatc ttaaattatc ttctaccaat
    28441 gttcatcaac cagtcagaaa atattttcgt tttcgtatac agctcaagta ttttgcctgg
    28501 tatggttcct tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga agcacgcaga
    28561 taaaatgcat gatcatcctt ttaaaatgcc actatctcct tatacgaatt atgtgacact
    28621 tgctttttta gctatcgtat tgattttcat gtttatcaat cctgaaacga gaatctcatt
    28681 gattattgga ctcgttttct tgctttacat gacgatccat tactttgtac gcgaaaaaag
    28741 aaccggaaaa taaaaaaaga acgaagcttc accttagtgg aagcttcgtt cttttttatt
    28801 gcttttatca attcaatttt agcgtctcac cgggagtcag gaaataattc tccatgctca
    28861 tcccattcag cgacaagatc gtttctactg gtacgccagt tcgttcggct atttgatttg
    28921 gaccttctcc ttccaaaact acggttgtcg cagcctgtga agtcgcgctg ttggtattag
    28981 tttcagagga atctgttgtg tcttgtgttg ttcgagcttg catttcagaa gcttctaccg
    29041 actctgctgt ctcagaatca gaagatgttg tgtcttgtgt gcttgttgac tctgcttgaa
    29101 gagaggcgct agtctgcaaa taaatatcgg aaatcttagc gacatgattc gtataattag
    29161 ccgataattg attgatcaaa gtatctaagt ctacaccgtc tttattttct gctgcctgat
    29221 acgctgcacg taattttctg atcccttcgt cggagattgc ttgaagctca cgtgcttttt
    29281 ggttagcaac tgctgataaa ccagctattc cctcttgatt tccctgaatt tctgtctggt
    29341 attcttcaat caatgcaggc gtcttttctt cgatcttttt cgaataatca tttacgattg
    29401 cttgaagttg ctctggcaaa ccatttgctg ttttttcttc agaagtttgt gtctcttttg
    29461 ttttagttga tgactcactt gtttgtttcg tctctgtttt ctttgtcgtt gtttccgtta
    29521 tggtcgtact tttcgcttct gacgaatttt ttccatgaaa atatagaccc gcaactcctc
    29581 ctgctatgat caaaaacacg gcaactgctg ccccaatcat gatcatccgc ttacgagctg
    29641 cctgtttttg ctgtcttctt cgggtcatcc gaacattttc tccccactca ttttcatcat
    29701 acattttttt caccaccaat ttttatgtat aatagcgtta tcaaacgctt catattctta
    29761 ttgtagcaaa taattgtcgc tgtgcacatt tttatctttg ttatttataa gaaaatttca
    29821 ccagttgaga tgattccaag attgtccgtc gatttctcct tgcagctgca ttgttcgttc
    29881 gtctatctca gccatcagtt tctttatttc atataagagc gcacggtctt gatattctga
    29941 tttttcgctt aattcatcta attgttggat cagccaatca tttgtcataa tgaagttcct
    30001 ttcatttctt gcagtgtaaa ggtcaagtct tcgatctgct cttttgcctc agtcagtgct
    30061 tctacgatat atgcttgttt ttcagattct tcttcctgca ttttctgatt ttcctgtact
    30121 ctttgaataa accagtcaat ccgctgagtc agcgatggtt tgccttccaa tgagagatac
    30181 tgctgataag caattagctt ctcccaacgt ttttcctggg ataaatacat aaattgtgct
    30241 aaaacgaatg gcggaagata ggtcatatgg aatttatttg cgattcgctg atacggcctc
    30301 aaaaattcgg aaatcgtata ttcttctttt cctccagctt ggtattcttt ttccgccaca
    30361 cctgttgtca atacgattcc tagttctttc ccttttaata catgtgctgc tcgttccaag
    30421 acattatctt gccaaatttt taaatgagca ggagaactat accagtaaaa cggaaactgg
    30481 aaaatgatcc gatcatgctg ggccaagagc tgtagttcag tctctatatc tatttttccg
    30541 tctggataac ttgattccaa atgatgccaa gtagctggta aaccagcaga agcttctttg
    30601 aaaaattgct gatttaaaga ttcttcgatc gttggatggc taatgatgat aagtgtacgc
    30661 atcccttttc ctccttttta gtcttcgctt tacagtgtag cataaccgcc acaagtttct
    30721 tttgccgatg cttgtcattt taaccagact aatcaggttg tccaagaaac tgcggtttgt
    30781 ttttccaact gttttttcaa ggtatccaaa gcaatattct taaaattttc gtttttcata
    30841 cgtttacgat agttcgtagc cgtttctccg gtactctttt tgaacattcg gctaaatacg
    30901 actagactat tgaaccctac ttgataggca atactttcca cggattcatc cgtcagagaa
    30961 agaagaaacg cccctcgatt gatccgtacc ccgatcaaat attccttgat actggtaccc
    31021 atatgatttt tgaatactcg cgacaaatgg ctccgattga ccgctaaatg ctgggcgatt
    31081 tcctcaacag acaatttttc agcataatgt gcctcgatgt atttgactgt ttcaacagct
    31141 aaagaggaat gaaccttttg tacaccaccg ctgacctttc cgccatcttt taaaaggact
    31201 gataaaaaac ggtaggttaa tgctgttaac tgtaattcat tttccaaggt ttcttccgta
    31261 tctttcatgc attctaaaat aatgtcgata tatttttgta tgtcagaaga gaccatcgtg
    31321 tattggtctt ctctgaaaag cgactgccga atgatttctt ctgcagcact cccaccaaat
    31381 gaaagccagc aatacatcca cggatcttct ccatctgata agtaaaacgt cgagtctcct
    31441 ggacgaatca agaacagatc accttttttc aattggaatt gttgattttt cacatgataa
    31501 gatcctttgc cttccatcac gacatgcagg atgtaatcat tgcgaatagc aggaccaaaa
    31561 gaatgaaacg gcatcgtttt agagaaacca caaaaatcaa aatacaattc tttttctact
    31621 tgttgatgtt ccttttttaa aaaaatcgat tgctccacgt ctatccctcc ttactccttt
    31681 agtataatgc aacaaaaagt taaataaaag tcacagaaat atattaaaaa ggaagcgatt
    31741 tcttgttaag tttaagtcat taaaagatct ttgttattcg ttgtaaccgg tttacttagt
    31801 attatatatt gaacaggaga ggaatacaga ttatgaaaag caaaatgatc caacgtgtgt
    31861 cctacgcttt aggcgctttt ggacacgaca cattctatgt tacattaagc acttatttta
    31921 tggtattcgt gacaagccag atgttcgcag gaacagataa ggcaacgaat gcaaaaatga
    31981 ttgctacagt tacaagctta gttgtaggta tccgcttagt tgaaattatt tttgatcctt
    32041 tgattggtgg catcatcgat aatactcgta caaaatacgg taaattcaaa ccatggttag
    32101 ttatcggcgg cgttgttagt tccgtactct tggtagcaat ctttaccaat ttctttggct
    32161 tagctactca taatacgact ttatttatca tattatttgc aatcgttttc atcttattgg
    32221 atagttttta ttcatttaag gatatcgctt tttggtcaat gattccagct ttgagtacgg
    32281 aaacaaagga acgtgaaaaa tttgctacgt ttgctcgttt cggctcttct ctaggtgcaa
    32341 atggtactac tctagtcgtt gtccctatcg ttactttctt tacctatctt gttactcata
    32401 aacatattga aggcgcttca ggctggttct ggttcggtgt gattgctgct gttatttcag
    32461 gcggtactgc gattatcact gccttgggca caaaagaagt cgatactgcg atccgtcagc
    32521 aaaatgaaaa agctgggatc aaagatgtgt tcaaagcagt tgctcaaaat gatcagttga
    32581 tgtggcttgc ccttgcttat atcgcttatg cgattgccaa tgcagcaaca actgccgtat
    32641 tattctactt cttcaaatat gtacttggcc aaccaactga attttggatc gttggtctag
    32701 ttgctgcctt acttggttta gtagctgttc cactattccc aatgcttaca cgcgtcatct
    32761 ctcgtcgcta cgtttacctt ggagggatcg caatgatgat ttcttcttac tttttcttta
    32821 cgatcgcagg ttcaagtttg ccaatcgtta tcatcgggct agtattgttc tacttccctc
    32881 aacaattgat tttcttatcc gctttgatga cgatcacaga ctctgttgaa tatggtcaat
    32941 ggaaaaatgg tatccgtaac gaagctgtta ctctatcaat tcgcccttta ctagataaaa
    33001 tcgctggtgc tttgtctaac gggatcgtag ggtttgtcgc tatcgcttct gggatgactg
    33061 gtaatgctac tgctgctgat atcactgctc atggcgtaac gactttcaaa gcttatgcat
    33121 tctatgctcc tgctgctttg atgattttct ccgctattat ttttgcttgg aagattacat
    33181 tgacagagaa aaaacatgca caaatcgttg agaaattgga aaacaaattg gctccagctg
    33241 atacaatgga agattaactt cacgatgcag tagttccttc ccattaagaa aggatcttga
    33301 aaatgaagat tacagaaaaa gattttggcc aaggttatca cttgataacc ttggagaaca
    33361 aaaacaagct agcactttct atctctgatt taggtgctag aatcgtcagt ttaaaatcaa
    33421 atgatcgaga attagttttg ggatttgaca ccgctgaaga atatatcgaa aaagatccgt
    33481 atatcggtgc ttctatcggt cgcaccgcag ggagaatcga aaatggccgc ttttctctca
    33541 atggaaaaac ttaccaatta gccacagatc caaaaactgg acataatctg catggtggtg
    33601 ctcctggatt tgaattaaaa aaatggtctt atgtcatttt gaatggcgaa aatgaagctt
    33661 ctgtcatttt tactactaca agtcccgacg gagaacatgg ttttccaggt acaatggatg
    33721 tggaaatccg ctatactttg acaaaagata atatctggcg agtgacctct cgcggcacca
    33781 gtgaccaaga tactttattc aatccgacaa atcatgttta cttcaacttg acgggagatg
    33841 cttctcagtc aatcgatcaa catgagcttt ggctcaatag cgaagcttat gctcctttgc
    33901 gaacagatag tattcctata ggggtaaaag aaaatgctgc aggaagtgct ttcgattttc
    33961 aaatacctaa aaaattagcg tccgtttttg ctagtgattt ggatcaaaaa aatcttgttg
    34021 acggaatcga tcatccattt ttcttaaaag aaaccggctt gggtaaagaa gccgctcgct
    34081 tgactagccc cgataaaaga atccaagtag atattgctac agatgcttct agtgtggtta
    34141 ttttcactgc aaatttcggc acagaaacac cagaaatgcg caaccgaaaa ctggcacatc
    34201 acggtggcat cacatttgag actcaaacag ctccaggtgc agagcgcttc tcagcatttg
    34261 gttcgattca tttaaaagcc ggatcagtat ttgaaacagt aacagaattc aaaatcaaaa
    34321 caagaaagga ataactcatc atggaaatca aaagaagctt aaacacaaaa tttgccgaaa
    34381 cattcggacc taaaaaaact acacaatact tctcaccagg acgcatcaat ttgattggtg
    34441 aacacacaga ttacaatgga ggccatgtct tccccgcttc catcacttat ggaacgcaag
    34501 gtgtagcagc acctcgagac gataataaaa tattagttta ttccacaaac tttgacgata
    34561 tcggtgtcat tagttttact ttagatgagc tagaatatga taaaaaagca aactgggcaa
    34621 attacgtaaa aggaatggtc cttaaattaa aagaagctgg acactcaatc gatcatgggt
    34681 ttgagttatt agtggaagga acaatcccta atggtgcagg actttccagt agcgcttcat
    34741 tagaattatt agtaggtgtt gtattagaag atctgtttga tttaaaaaca gatcgcttgg
    34801 aactagtcca aacaggtaaa aaagtagaaa atgaattcat cggtgtcaat tcaggtatca
    34861 tggatcagtt tgccatcggc ttcggtgagc tagatcatgc aatcttatta gatacaaata
    34921 cgctgaaata cgaaatggtt ccagtgaaac tagatggtta tgcaatcgtc atcatgaata
    34981 cgaataagcg gcgggaattg gcagattcaa aatacaatga acgccgtagc gaatgcgaag
    35041 aagccttggc acgtttgcaa aaaactttgc ctatctcttc tttaggagac ttagatgaag
    35101 aagaatttga atcaactatt gaccaaatcg gcgatgatac attgatacgc agagctcgcc
    35161 atgccgtttc tgaaaatcag cggactttga aggcaaaagc tgaattagaa gctgggaact
    35221 tagaagcatt tggtcaactg ttaaatgatt ctcaccactc tcttcgctat gattatgaag
    35281 taactgggat cgaattagat acactagtag atgctgctca aaaacaagaa ggtgtcttag
    35341 gtgcacgaat gacaggagct ggatttggtg gctgtgcgat tgctcttgtc aaagaagaaa
    35401 atatacctgc cttcaaaaac aatgtctatg atgaatacat gaaagttgtc ggctatgctc
    35461 cggaatttta tgttgcacac attggaaatg ggacaacaaa gatcgatcca gaagctgttc
    35521 atgtttgata aaaaaatcaa cgatctcagc taaaaacaaa tagataaaaa taacctctca
    35581 gcaatttcca aatgtgagcc ttttgctcgt atttgaaaat tgctgttttt tattgacacg
    35641 gaaagagaac gtgtgttcta attgtgttga agaggtgatt gttatgtggg cgaatcaaaa
    35701 tccggtattt gtttatgaaa aagaaccttc aagagatatt ttatgtattg actgcaaatc
    35761 tttttacgcc tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa ctaaattagt
    35821 agtcatgtcc tatccgtccg attcgacaga aacacggggt agcggattaa tcttggcttc
    35881 cagtcctgca gcaaaaaaag cttacggcat caccaatatc agccgtgcca gagatcttcc
    35941 cttcccctat ccaagtgatt tatatattgt tgccccaagg atggcctatt atatggaaaa
    36001 gaacagagaa atcaattcta tctataaaaa atatgcagac gaagcgaacc atcatgtcta
    36061 ttccgtcgat gaaagcttcc ttgatgtaac aaatagcatg gcactctttg gtaaaacaaa
    36121 tgcgtacgac atggctaaac tgatccataa agatgtgttt gagcagaccg gaatctacac
    36181 gactatagga attggcgata acccgctact tgctaaacta gccttagaca atggctcaaa
    36241 agacggaaag caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa agttatggaa
    36301 aatctcagat atcactgact tttggggaat cggcaaacga acagcaaaac gtctgaaaca
    36361 aatgggtata cattctgttt atgatttagc tcacgcggat tattatcact tgaaaagcaa
    36421 gctaggggtc atcggtactc agctttatgc ccatgcttgg ggcatcgatc gaagtttttt
    36481 gggacaagaa tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc tagatagaga
    36541 ttatacaaga cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc aagtcggcac
    36601 tcgcttgaga cgatctgcag caaaaacaca agtagtcagc ttatgggtag gatactcttt
    36661 aggctatctt gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa tcgaagcgac
    36721 aaatgctagc agagatcttg cagatgcttt attgaccatt ttccaccagc attaccacta
    36781 ccaggatatt cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga caggtttaca
    36841 gcttaacctt tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg attttgttgt
    36901 cgatacgatt agaaaaaaat acggctttca atcaatcatc catgcgcata gtctcttgca
    36961 aggaggacgc gccatcgcac gtagttctct cgttggcggg catgcaggtg gtatgagtgg
    37021 attagaaggt ggaaaagatg agacggacag caaaaaaatt cattgactat aacgagtatc
    37081 gagatcggcc atttggatta aaatggggga ctgcttttgc gatggaagaa ctagtcaacg
    37141 gtatcaaaaa aatgaagaag aagcacgcaa acagcctctt cctaaacaac aaatgacatt
    37201 gcaagaaatc gatagtatac tgacagaagc ctttttatct cgtaaagcag taatcattca
    37261 gctcaattta aaagatgaat ttggaagact cttggatgag gtcgaagggc acttcattgg
    37321 tgaagcctat gaagattact ttatgattga ccatcagccg atattgtggg aagacgtacg
    37381 gtatattgag ttaaaggaaa caaaaaaatg gtttgtgaca tgagaggttg tgacagaagt
    37441 gatcaacttc aaaaaataag aaaggatttt tcgaaaattg cttcttaaat ttttgtggaa
    37501 ttttggctta tttttcgaag aagcaacttc tggaacaacg tatattagat atcagaggtt
    37561 gtgaaaaagt cgcttagttt tgagtaacaa gccaaggcgc tgatttcaca acctctgtct
    37621 atctatccat ctattcggta agattcatgc atgacgtctt ctttatttag tatgtagagc
    37681 cctttttctt ctgaccattc aacataaatc agctgtttta catctgcata gccttctttc
    37741 ttcaagttct ccataagcca tgcttcagtc aattgattgg actgaagcac tttatgctga
    37801 atttgccctt catctacaag caggatcgat aaagctttgt cttcatctga ttttttcaat
    37861 acactcattt gaccggcatt ctctaggata acatgtttgg cattttcaag aaaaaagcat
    37921 tcttgctgtc ttaagattgc tcgcaactgc tccatctcga tatggttatt tgtcaactct
    37981 gtcatgttga tgacgccgtc tttgattaga acagatggct cgcctttgac ccaacgattg
    38041 accttttcat ttttttggac gatacgttcg ataccataaa tcattactgc ccaaagcgca
    38101 atggcaaata ggacatgccc tacatggaca ttgtcatcat aggttgattc ttctaagatc
    38161 ccacctaaaa caagcgtata gactaaatca aacggtgtga tttctgacat ggatttcttc
    38221 ccaagcaaac gtaccactaa taataaaccg atcaacccga acaccagttt cattgtaatt
    38281 gaaaaaatca ttttttctcc ccctaactat ctgttaatta aattatatat gctttctcac
    38341 gaacacgcat ggaatataca aggaagaaat gtatcctttt actaaaaaaa ggactgtgac
    38401 actgtagtga cccccaaaag ttagactttt ttggagtgga gaaatccact cctttttttt
    38461 atgctctaaa atgatatttt tacacaaccg ttacggttgt tttacgaaat tggacg
//
LOCUS       DAYYHB010000019        36569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000019 DAYYHB010000000
VERSION     DAYYHB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.019"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA6B_000909"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA6B_000909"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8948499.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA6B_000910"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA6B_000910"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8948500.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA6B_000911"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA6B_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8948501.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA6B_000912"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA6B_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8948502.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA6B_000913"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA6B_000913"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8948503.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA6B_000914"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA6B_000914"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8948504.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHA6B_000915"
     CDS             complement(8053..8523)
                     /locus_tag="ACHA6B_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8948505.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHA6B_000916"
     CDS             complement(8569..8994)
                     /locus_tag="ACHA6B_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8948506.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHA6B_000917"
     CDS             complement(9061..9330)
                     /locus_tag="ACHA6B_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8948507.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHA6B_000918"
     CDS             complement(9341..10936)
                     /locus_tag="ACHA6B_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8948508.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA6B_000919"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA6B_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8948509.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA6B_000920"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA6B_000920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8948510.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHA6B_000921"
     CDS             15402..16376
                     /locus_tag="ACHA6B_000921"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8948511.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHA6B_000922"
     CDS             16546..17946
                     /locus_tag="ACHA6B_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8948512.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHA6B_000923"
     CDS             complement(18024..18746)
                     /locus_tag="ACHA6B_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8948513.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHA6B_000924"
     CDS             complement(18825..19331)
                     /locus_tag="ACHA6B_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8948514.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHA6B_000925"
     CDS             19454..21253
                     /locus_tag="ACHA6B_000925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8948515.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHA6B_000926"
     CDS             complement(21321..22895)
                     /locus_tag="ACHA6B_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8948516.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHA6B_000927"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHA6B_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8948517.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHA6B_000928"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHA6B_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8948518.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHA6B_000929"
     CDS             complement(24672..25310)
                     /locus_tag="ACHA6B_000929"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8948519.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHA6B_000930"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHA6B_000930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8948520.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGSNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHA6B_000931"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHA6B_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8948521.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHA6B_000932"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHA6B_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8948522.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHA6B_000933"
     CDS             complement(29104..29844)
                     /locus_tag="ACHA6B_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8948523.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHA6B_000934"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHA6B_000934"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8948524.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHA6B_000935"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHA6B_000935"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8948525.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHA6B_000936"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHA6B_000936"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8948526.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHA6B_000937"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHA6B_000937"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8948527.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHA6B_000938"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHA6B_000938"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8948528.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHA6B_000939"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHA6B_000939"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8948529.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9887 a         7989 c         6705 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tgaccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAYYHB010000020        35401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000020 DAYYHB010000000
VERSION     DAYYHB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.020"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..380
                     /locus_tag="ACHA6B_000940"
     CDS             81..380
                     /locus_tag="ACHA6B_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948530.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            645..1520
                     /locus_tag="ACHA6B_000941"
     CDS             645..1520
                     /locus_tag="ACHA6B_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948531.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1517..1735
                     /locus_tag="ACHA6B_000942"
     CDS             1517..1735
                     /locus_tag="ACHA6B_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948532.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1722..4349
                     /locus_tag="ACHA6B_000943"
     CDS             1722..4349
                     /locus_tag="ACHA6B_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8948533.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4364..4582
                     /locus_tag="ACHA6B_000944"
     CDS             4364..4582
                     /locus_tag="ACHA6B_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948534.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6101..6415
                     /locus_tag="ACHA6B_000945"
     CDS             6101..6415
                     /locus_tag="ACHA6B_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948535.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6468..7241)
                     /locus_tag="ACHA6B_000946"
     CDS             complement(6468..7241)
                     /locus_tag="ACHA6B_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948536.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7346..7591
                     /locus_tag="ACHA6B_000947"
     CDS             7346..7591
                     /locus_tag="ACHA6B_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8948537.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7747..8598
                     /locus_tag="ACHA6B_000948"
     CDS             7747..8598
                     /locus_tag="ACHA6B_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8948538.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            8631..9308
                     /locus_tag="ACHA6B_000949"
     CDS             8631..9308
                     /locus_tag="ACHA6B_000949"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8948539.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            9796..10761
                     /locus_tag="ACHA6B_000950"
     CDS             9796..10761
                     /locus_tag="ACHA6B_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8948540.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            10783..10995
                     /locus_tag="ACHA6B_000951"
     CDS             10783..10995
                     /locus_tag="ACHA6B_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8948541.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11070..11546
                     /locus_tag="ACHA6B_000952"
     CDS             11070..11546
                     /locus_tag="ACHA6B_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8948542.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            11543..13351
                     /locus_tag="ACHA6B_000953"
     CDS             11543..13351
                     /locus_tag="ACHA6B_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8948543.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            13428..13643
                     /locus_tag="ACHA6B_000954"
     CDS             13428..13643
                     /locus_tag="ACHA6B_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8948544.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13735..14598
                     /locus_tag="ACHA6B_000955"
     CDS             13735..14598
                     /locus_tag="ACHA6B_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8948545.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            14635..15171
                     /locus_tag="ACHA6B_000956"
     CDS             14635..15171
                     /locus_tag="ACHA6B_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8948546.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15185..15607
                     /locus_tag="ACHA6B_000957"
     CDS             15185..15607
                     /locus_tag="ACHA6B_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8948547.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            15537..17936
                     /locus_tag="ACHA6B_000958"
     CDS             15537..17936
                     /locus_tag="ACHA6B_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8948548.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17968..19959
                     /locus_tag="ACHA6B_000959"
     CDS             17968..19959
                     /locus_tag="ACHA6B_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8948549.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19982..20233
                     /locus_tag="ACHA6B_000960"
     CDS             19982..20233
                     /locus_tag="ACHA6B_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8948550.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20223..21452
                     /locus_tag="ACHA6B_000961"
     CDS             20223..21452
                     /locus_tag="ACHA6B_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8948551.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            21449..23530
                     /locus_tag="ACHA6B_000962"
     CDS             21449..23530
                     /locus_tag="ACHA6B_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8948552.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            23679..27599
                     /locus_tag="ACHA6B_000963"
     CDS             23679..27599
                     /locus_tag="ACHA6B_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8948553.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            27600..28544
                     /locus_tag="ACHA6B_000964"
     CDS             27600..28544
                     /locus_tag="ACHA6B_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948554.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            28548..28751
                     /locus_tag="ACHA6B_000965"
     CDS             28548..28751
                     /locus_tag="ACHA6B_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8948555.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29051..29287)
                     /locus_tag="ACHA6B_000966"
     CDS             complement(29051..29287)
                     /locus_tag="ACHA6B_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948556.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(29470..29841)
                     /locus_tag="ACHA6B_000967"
     CDS             complement(29470..29841)
                     /locus_tag="ACHA6B_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8948557.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29838..31166)
                     /locus_tag="ACHA6B_000968"
     CDS             complement(29838..31166)
                     /locus_tag="ACHA6B_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8948558.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31127..31456)
                     /gene="mobC"
                     /locus_tag="ACHA6B_000969"
     CDS             complement(31127..31456)
                     /gene="mobC"
                     /locus_tag="ACHA6B_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8948559.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31714..32084)
                     /locus_tag="ACHA6B_000970"
                     /pseudo
     CDS             complement(31714..32084)
                     /locus_tag="ACHA6B_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            32229..32414
                     /locus_tag="ACHA6B_000971"
     CDS             32229..32414
                     /locus_tag="ACHA6B_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8948560.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            32572..32739
                     /locus_tag="ACHA6B_000972"
     CDS             32572..32739
                     /locus_tag="ACHA6B_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948561.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            33357..34016
                     /gene="vanR-B"
                     /locus_tag="ACHA6B_000973"
     CDS             33357..34016
                     /gene="vanR-B"
                     /locus_tag="ACHA6B_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8948562.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            34019..35380
                     /gene="vanS-B"
                     /locus_tag="ACHA6B_000974"
     CDS             34019..35380
                     /gene="vanS-B"
                     /locus_tag="ACHA6B_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8948563.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT        10030 a         8143 c         8878 g         8350 t
ORIGIN      
        1 ttttttacgc aatttgtatt tatttgtact tataaaggta gaggagttat agaataatta
       61 attaaaaagg agaggttttt gtggaagcaa taaaattaat gatttcagaa gaacaggagg
      121 aaatgtttct aaagcagttt tatcaaattg ctacaaaagc tattgaaaag gctcaagaaa
      181 atgcggggat gacaagggaa tatctcaatc aaaaagaaat ggcggaatac ttaaatgtga
      241 gtgaaaactt tttaaaaaat gctgtgagaa atgagggatt gcccgtagtt gaattagggg
      301 caagaaaatt ttattcgaaa aaggcagtta atgagtttat gtttgctcga atgaaaagag
      361 gggaaaatat tggaagatga tgaagtagaa attataaaaa aatgctttaa aaaaagaaaa
      421 aaagtgttcc caaaagtttg cagactgggg gaacactcaa taataaagtg ctgagaaaac
      481 tttatttata gctattatat caaaaaagaa ggtgaaaagg tgttttcttc tggaaatgtt
      541 gtagttgaca aacttagaga aataaatttt gacggaaatg taatccacac acatggtacg
      601 atcaattaag aaaaaaaacg aaaaaaggtg ttgagaagcc ctatttgaat gcaatagtta
      661 tcttagcaga aattacctat tggtatagac ctaaaaaaat ttttaacgat gatggtcagc
      721 tacttggtta tcaaaaaaag tttcttgaag atattttgca aaaatcatat aagcaattaa
      781 gcaaaaaaac aggattatca gaacgagcta taaaagatgc aattgtgtac ttagagaaaa
      841 aggggattat caaaagggtt tttagaaata tttattatga gaaaagagca gttacattat
      901 caaatgttat gtacattgtt ttaaatgtac ataaattgat agaaattact tttcctactt
      961 tggattgcga aaaaaaggag tatatgccga aaattcgtca gaggggtatc gaaaaaaaga
     1021 gatatgtctc tttaaaaaaa ggtagaggta tgtacaaatt tgtatatacg tatacaaaga
     1081 ctacttcaca gaacatatca aagactactc tcgttatgga agaagaggat attaatataa
     1141 agaggattta cagtaagatt agtgttcttt atgacgcaaa taacgaagat ggtcgaaaat
     1201 tatttcaagt gtatcactat ttgagaaacg ctaatgtaga tacagaagat atcagaaaaa
     1261 tcattgaata tttatctgaa aatactcagt atcttattgc tgagtgtatt ataaaacaac
     1321 atgaagcatg cttttttcaa agaaaaactg aagaagggtt atacgattat gtaacatatt
     1381 ttttgaatgg acttcagaaa agatataaaa gtcaattttt agaaaatgta gagcctatag
     1441 atcagttcaa aaaagctttc gaaatcaaag agttacctga aattactatg cataattggt
     1501 tgaaaggaga agaatcatga ttaaaaatcg cttaatagat caaatgtggt gtcttgatca
     1561 aaatacagat actctcgaat taatcgagag tatctgcttc aacacagtag ttctcgattt
     1621 aagagctgaa aatgacaatt gtgtaactca tatcataatt aatcaaaaaa tggctcagca
     1681 gctctctgag agcctaagaa aatgggctga aggagaaaac tatgaaagca attaaagaaa
     1741 aaatcgttag tagaaaaata aactggttag cactcagtgt acttgtttta atgattagcg
     1801 ttgtttttac aagtaatatt gttgaagcta gcattttcaa ttggtttgat gacacaaaaa
     1861 gtcaaacaga gtttttgaat aattctcttt atagacctta cctaaaaaaa tacaatggag
     1921 atttccttag tcagttcggg atttggcttg gatgggctgt ggtaaaaggc atgtttactg
     1981 tgactgattc gattcaaaat atgattccag atgtgttgga tttgttcaat ttcattgaaa
     2041 gtacagggct aaataatgtt tatcaatcag tcataaatac aattgtcgtt ggattaatga
     2101 tcctttcatt gatgtttgtt ggctacaaga tgattactgg aaaaggaaca atagatttaa
     2161 gatctgttgg aatgaatata gtgatgtctg tagcgttaat tcttttaatg ccaacaatga
     2221 tttctagcgg tattcaattt gccaaaatat tttacaatga tgctactacg attacaaatt
     2281 cagatgacgg agtcgcttgg tcattgataa aacaaggagt aacagatctg gcatatatta
     2341 ataaaacaga tcaatactca agcattgata aaacggagga tcgaaataag ttaacacgaa
     2401 aaaattttca acagacagat ttaacacaag tgttgacgga taaagtgatt gataaattgg
     2461 aaaaagaaaa tccagctgcg gataatcttc gctatgaatt agttgaaaat tctaataatg
     2521 aatttgttgc cacaaagttt tctgataact tcttatcaac attttctgat agcttgaaat
     2581 caggttatta tcgatatcaa gcaaatcttt gggggatttc gattgggttg acagcgttgg
     2641 caatcgccta tgtgttcagc gcgtttgtga tcattacagc aatattagaa cttgcattta
     2701 aacgtgtgtt aggtgttctt gtttttgcga cagatattga aacaggacaa cgttcaaaag
     2761 ttgttttatc cgatatttta caatgttact taacagttgg atttcaaggg tttggactat
     2821 ctatgtttgc tatgtttatc aattttttaa atgctggaca aggtatctcc acaaatattt
     2881 ttataaaaac aattgcttat atctgtgctg tgtttgtgct gataaaaggc agtggaacag
     2941 tcatgagata ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg
     3001 cttttggtat gggcgcaatg ctgtttagaa aaggatcaac tggatttaat cgtgctaaag
     3061 gaagcggaaa tggaaatgga tcagacagtg gagaaggaga agatcgaaaa ccagaaaaga
     3121 atttcggcga aactctatca aaaaaagctg gcaaaactgg aagagctttg ggatatgccc
     3181 atgaacgtgg attatcaggt ttagcttcag atggagctac aatggctagt gaaagagcaa
     3241 taaaaccatt taagagtatg cgtgatatgg ctaatgatac caagaataag ttcaaagaag
     3301 gtttagatga tggtacagtg tccgctatta ataaaaatag caaacctatg ttagctaaaa
     3361 ataaagaaga tgaaactgga aaatatgctg attccatgcc aacacgtctt tcggatagaa
     3421 aagatggtgc taaggttgaa aatgccgata gaattatgtc tagttctgaa cgtatgcgtg
     3481 aagcaatgaa aaataatgct gaatcgaata ataatcctgt aagtgcaatt cagcaaaaag
     3541 ttcaacaaga tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag
     3601 aattaattaa tcagaagcga caagaagcta aatacacgcc tgaagcaatg aatcgtgagg
     3661 aaatgttaag gaaacgtgta gaagggcgta caggagctaa tacggacgaa aaagaagcgt
     3721 tgaccaaaga aagaatccaa gaagctaaaa actcaaatac cggattagaa aaattggtca
     3781 aagaaaatct acaaaattct acaagtagtc aaggtggccg atcagtagat gtccgagaaa
     3841 atattcaagg ttcattgaat ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac
     3901 aaagctcttc aaatggtcaa ggtactaaga caattgatgt tcgagaaaat gtacaaaatt
     3961 ctacaagtag tcaagggttt aaatcaatgg atgtaagaga gaatgtacaa aattctacaa
     4021 gtagtcaagg gtttaaatca atggatgtaa gagagaatgt acaaagttct acaagtagtc
     4081 aaggattcaa aacagtagat gttcgggaga acgttcaaaa agatagtgga gaaatgaaag
     4141 aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa
     4201 atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa
     4261 gacgaagatt tacctatgaa gacaacgagt tattcagaga tacacttaat gatcctaatc
     4321 cgcttttcga taatctatta aaaaaataaa agggggaatt gtcatgaaat taattctaaa
     4381 gattagtacg catgatttct tagaacttga cgaattatgt gaatcagtaa agaacgcaac
     4441 aaatgaaaaa atcacggtca ctaattatat tgaactgatg attcaagaag aattaaagat
     4501 gaaacatgag ataaaaaaca acttgaaata ttgttatcta gaaaaaagtg aagagataat
     4561 ccatgaatta aatggctttt aaaaattgta tttgacatga atttacgaca aggaattagt
     4621 ggggattaag cgattattct cttccacttt agagaaataa tgggttaaaa agccagaata
     4681 cgtagagcaa tattgacaac ctataaagga gctattgttg aaaattgaag taacaaaaag
     4741 ataaagggag gtttacatct atcagattaa aagtgatagt acacccatga aacctgcaaa
     4801 agagttgaac ctaatgtata tcggtttaac tctttttatt tgcaaattaa aaaaatatca
     4861 ccgaagtgac gaatgaaact atgaccaaat taagcaagaa aatatatctt gcaaataaag
     4921 tagtataaaa agatattgat aaagccaatc aaaactttga atttcaaacg ttagataatc
     4981 aaacagatac aaaaaatgaa atcagttcta cgcgtacgat tattaaaaat aataaatttt
     5041 cactagaaga aaatggaaat aaaatcaaat aattaggaag aaaaaacaaa gcaattagaa
     5101 aagcctgcaa gcaaatttga gtaatcgaga tccaattttt ttaaggaagt ttaacaaaaa
     5161 tatgtaattc tattatcact attaaagaga aagttggagc ggaatataaa gaaaagagaa
     5221 aagtaattaa acgcaaaaaa atgtagaact taaaaaatat aaagaggaac aaccgttaaa
     5281 attaggaatg atgttgtgta aacattataa ttgctatgat ataaatgaag tgttaaagaa
     5341 agcaaattta aaccaatttt gtgaacagaa aagccttagt cactatgacc aaaactacgg
     5401 acgaaactat gaccaaaagt aggtactaaa ttatgaccga aaataggtcc aaaatggatt
     5461 tttttatgat atcaaaaatt atgaaatgat tttgaaagaa aaccagttgg cacagccaac
     5521 ttagccacct tcctgtagga aggtgctatc agttttccct tatgctcttt tctttctttt
     5581 ttgtttcata tgtcaaatgc cattttgaaa tgtaccatct taagaaattt atccttaaaa
     5641 acaattgcgt tattggataa aaagatgcta cattatctta aaaaaggaat caaatatgat
     5701 gaatttagtg gctagcatat cgtgtgatta agatacttta tagtgtaagt tgaaggagaa
     5761 aaaatattta aaatatactt cccatttcag tgggaagtaa aattaatctt gttgtttcta
     5821 ctagtaatgt atctttttgg aaactatgta acgatgtaaa caagattttt ttcagaagaa
     5881 aaaacgataa aagttgtgat aaacgattta aaaaatagga gaaaatatat caagcgcatg
     5941 gtatgaaacg cacatttaaa aagattcgac taactaaaaa aataagttta caaagcattg
     6001 ttgtgtatta caagtgatct atacactgaa aaatgataga gaaaatctga tacgtccgca
     6061 acaatggaca attgaaaatg aaagtgagga aattggaaga atggctgatg aaaataaagt
     6121 tgttccgctt gaaacactag tgataaataa ggaagagttt gagcaacttt ttcttgattt
     6181 ttttgttcaa tatgacagga tttatgattt tagttcgtca ctagatttat ttgaattaga
     6241 ggcagaaata atagaaaaca gtcgagagga gaatggtaaa atcatcattt cagtagaaaa
     6301 attcaatgaa ttattctcag aaacatttga aatagataca gattattatc aaccacaaga
     6361 agttttccaa tcatttagaa atcagattat tcgctcaggt aagaccgtaa actgaaaaac
     6421 aataaaagaa acgttgaaag aataattatt ccttcaacgt ttctttttta gaatgtttta
     6481 atgaccaaag cagtttcatt gtcaggctta ggtaattcat gaaatcccgt atttatagtg
     6541 ctcaattgat caactaacca tgttttactt tttgaattgt agataaatct aaaattaata
     6601 tcgttattat cgtgttgaag gggcgtctcc aaataatctg ctgtatattc atagtcaacg
     6661 taacctgcta atacaaaaga gagtatccct ttatttaatg ttagatttaa tggataatca
     6721 tacgacttta tttttttcaa cttcatattg tacatatctt cagccataaa agttgagttc
     6781 gtgatttcat cttgggtctc attaaaaaag ttatccgaat aatcgcttat ttttgaaaag
     6841 tctttattgg caccagcctc aaaaaattgt tcaacttttt cagtataaag ttgtgctaac
     6901 gctccttttt caatattaac tttaatattt tcatgtctat caatttgtgt gctgttcagt
     6961 aagggaagat cagcttttag ttttatatcg tcttgatttc tttcaatttc aattggcaaa
     7021 atcttttcag tagattcagt ttctgataaa ggaacatcta gttttttatc atgataataa
     7081 gcttcaaatc catctggaac cggaaaaaaa tgtattgggt aattcttttc tgaggttgtt
     7141 gtactagttt ccgtacttgt agaacttgtg tcagaagtgg tacacccgct tatagttccg
     7201 atgagtaaag cgcaagttag aatgattgca aatttcttca aaaaaattcc tccttaaaat
     7261 tgaatatgga ctaatactag tcccattgta gcataccgaa tctttaagat taataagaaa
     7321 aaggactaat tttagtccaa aaggtgtgct attcttgaaa gaggaaaaaa ttattgataa
     7381 agaaaatgta atcggtagca atattagacg agtccgatta gaaaaaggaa ttggtcaaac
     7441 cgagctgatt agagacctac agcttcgtaa aattgctata actagggaaa cgttggttaa
     7501 gattgaaggt ggccgccaac acattaagtt ggatcaattg aaagccatca aagatattct
     7561 acaggtgtct tatgaagatt tgcttcaatg actacttgta gtcatttcgt ttgaaattat
     7621 tatgttacac ccttatgtgt cacgaagaaa tactggtata ataatgaatg ggatatgtaa
     7681 cgatagggta tactgttatg ttgcctaata aattgtggat caaaatacaa gaatggaggg
     7741 atatccttgt ataaggcata tgaattggat tttacaaatg ctgacttaaa tgcattctcc
     7801 tcaagctcgc aatcatatac acatgtagta aatcaaatag atcacaacca aaaatctatt
     7861 aacaagagaa tagaaaatct atttgaaagt gaaaatgatt tagttgatta ttattctaag
     7921 gacagcaaaa ttctagatgc agacgaaatt attaaagatt ggtttccaac tataaaagca
     7981 gatattttta tatctcactc tcatgctgat gaaaaattag ctatacgttt tgcatcatgg
     8041 ctatttgaaa attttggtct tactgcattt attgattctt cggtttgggg atacagttct
     8101 gatttattaa aaaaaataga ccaaaaatat tgctataagg aacaaacaaa aacatatgat
     8161 tatgataaaa gaaatgtaac aactagtcat gttcatatga tgctttctac agctttaaat
     8221 aatatgattg atagtacaga atgtctgttt tttcttaata cacctaattc aattagtttg
     8281 agcaatgaaa tcactaatga acagaaattt acgtattcgc catggctata tagcgaactg
     8341 acgacagctt caatagtgga aaaaaagaat ccacgattgg aaagtaatcc tcaaatgagc
     8401 accgaggatg ttagaagcat tataaagcac tattcttcgc aagaaaatct gcaaataaaa
     8461 tattctttaa atttaagtga attaacttat ttacgaaaag ctgatttgaa aaaatgggct
     8521 ttagatgctg tggaatgcac taaaggaagt aatacattag atattttata taaaaacacc
     8581 attttcacta atctctaatc aatttactga atcgaaagga accgggtaga atggataaga
     8641 aagttttaat aaagaggttc tctgaagcta ttgaaaaagg aaatgcagca atatttgcag
     8701 gtgcaggatt atcgatgtca caaggatatg taagttggcc ggaactactt aatgatcctg
     8761 caacagaaat cgggttggat tctaaaaaag aaacagactt agttactctg gctcaatatt
     8821 ataaaaatga aaatggtgga tcaagaggaa ttttgaatca gatacttatg gataattttg
     8881 gtgaagaatt agaaatatct gaaaatcatc gaatactagc aagtttacct attgaaactt
     8941 attggacaac taattatgat catctaatag aaaaatcaat ccgagaagct tataaaaacc
     9001 ctcaggttaa gaaaaattat actcagttag caacaactaa tccaaatgtg gatacaattg
     9061 tttataaaat gcatggtgat atagatgatg tcagttcgac agtaataaca agagatgatt
     9121 atgaaaaata tgatgattcg tacgcgttat ttaaagaaac tttaaaagga gatttgttaa
     9181 caaaaacttt tctcttttta ggatttagtt ttacagaccc taatttagaa agaattttat
     9241 cagatataag atgggttttg agagaaaacc aaagacctca ttattgtata atgagaaaaa
     9301 tattatagaa aaaattttag gttatcgctg aaagccccgg aaatacgggc atttcgcagg
     9361 atatgagaac tcaatttact accaatttac tactttcgga ttgagccgca tatatgctga
     9421 accatactgc taatacccgc caccccaaat acggcacatc ataaattgaa tacgacaccg
     9481 taaacgcggc gttcctcttt cccgtcatgg gagaacagga cgccgctttt ttatgcccaa
     9541 atagagaaag gaggcgacgc catgctattt acttccggcc ctgaccgggc gtttgaacgg
     9601 ctgatgcagg aaaagcccgg tttcgatcac tacgacggcg gcggggcgga cgcgccggag
     9661 gattgcggca cctgccgatt ttaccgcccg gattggaaat atcaattctg ccagtttgca
     9721 gagtgtccct atcagccggg caagctgacc gactatgcag cggtcaaatt cacagtgaaa
     9781 ggagcgaaca acgctatggc agttttccgt gtggaaaaga acaaaggcta tacggtgatg
     9841 tcaaatcacc acctgcggaa taagaacctg accttaaagg ccaagggcct gctgtcgcag
     9901 atgttgtcct tgccggagga ttgggactat accctaaaag gactgtccta tatcaaccgg
     9961 gaaagcatcg acgcgatcag gacagcggta tgggagcttg aaaaagctgg atatatccgg
    10021 cgtgagcagg gccgtgacgc aaaaggcaaa atggccgata tggtctatac catctacgaa
    10081 cagccggtat tggaaaaccc ggtattggaa aatccaacat cggataaccc ggtgttggaa
    10141 aatccgacat cggataatcc ggcgtcggaa aatccaacgc aattaaataa agaagtacaa
    10201 agaactgact tatcaaaaaa agaaaaaata attacagatg gacaaagtac ccattccatt
    10261 cctatcccat ccccgacccc tgcccctatg gagggcgagg cagcggctac gccgccggaa
    10321 cggaaaagga caggaaagga tgaggctgta cggatttatc gggaaatcat tttggagaac
    10381 atcgagtatg cctatctcat tcaggacagg agcattgacc gtgaacagct tgacgaaatc
    10441 gtagacctga tgctggaaac cgtctgcacc gcccgaaaga caatccgtgt cgccggggac
    10501 gactaccctg ccgagctggt gaaatccaag tacatgaagc tgaccggcga acatatccgc
    10561 tttgtccttg actgtctgcg ggagaacaca acgaaagtcc gcaatatcaa acaatacctg
    10621 cgggctatgc tgttcaacgc ccccagcacc atttccaact actacacggc ccttgtcgcg
    10681 cacgacatgg cacagcccga ttggggaaag cctaaatccg gcctgccgga ctattcctgc
    10741 ggcgagggcg aaagcctgta acgagaaaag gaggacgacc ctatgaagca gggcgcattg
    10801 atttttgacg agcgaacaga ccgttacgac atccgctttg atctggccga ctattacggc
    10861 ggcctgcatt gcggggagtg tttcgacgtg atgatcggcg gcaaatggag gcccacccgc
    10921 attgaaatgg ccgaaaactg gtatcttgtc ggcatccggg ccgacgacct ttccggcctg
    10981 cgggtgcgga tttgacctat atctacctgc ggcgtctggt ggttccgcct tagacgccgc
    11041 ttttgacttt ctgacaagga gggatgcgat tgcaggaaga agttactcaa aagaccattg
    11101 ccctgtccat gaaaacgggc aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt
    11161 acctgcaaca ccgggccaag ggcaaaaaca cgctgcacca cggccagcag agcctaaagc
    11221 agcttaaaaa gcatggggct gatctctcca acattgagat caccgaggcc aacattggag
    11281 cgttcaagcc atgcgccaag aaatacggcg tggattttac cttacgaaaa gataagacca
    11341 cccagccgcc ccactacgtt gtgattttca aggccaagga tgcagataac cttgatctgg
    11401 cgtttcggga gtttaccgcc aagacgcttt ccaaggagca gcggccctct atccgcaagg
    11461 ttttgtccgc tgtgaagcaa aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa
    11521 tcaaggaaag ggggctggaa ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct
    11581 cccgtatctg atttttgtgt acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc
    11641 tggtgctgac ctgtcgggga agctcctgca ttttgcggat ggttttgcgg cggcttttgc
    11701 caaccctctg ccaagcctgc acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat
    11761 cgtgcggctg gtggtgtact gcaagagcaa gaacgtgaag aaataccgca aaggcatgga
    11821 gtatggcagc gcccgctggg ggacggccaa agacatagcg ccttacatcg accccaagtt
    11881 tgaaaataat atcctgctca cccaaacgga acggcttacc atgaccggca ggcccaaaga
    11941 ccccaagaca gcgcggaata aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac
    12001 gagatttttc gtgaaaccca acctgatgca atgctttccg accacggact atccgacttc
    12061 gtttgtggtg acagatccga aaggcacttt ggttctggaa accggcagga tgttccagca
    12121 ggcgggctac cgcattaaaa tcctgaatac cattaacttt tccaagtcca tgaagtacaa
    12181 cccctttgtc tacatccatt cggaaaagga cgtgctaaag ctggtaaata ccctgatttc
    12241 caacaccaag ggcgaggggg aaaagtcagc ggaggatttt tgggtgaagt cggaacggtt
    12301 gttttattcg gccctgatcg gctatatctg gtacgaggcc ccagcggagg aaatgaactt
    12361 ctcaacgctg ctggaaatga taaacgccag tgaagcccgc gaggacgacc cggagtttca
    12421 aagccccgtc gatctcatgt ttgagcgttt ggagcagaaa gaccccgacc attttgcggt
    12481 gcgccagtac aagaaattcc tgctgtccgc aggcaagacc cgttcttcca tcctgatttc
    12541 atgcggtgcc agacttgcgc cctttgacat ccgggaggta cgcgagctga tggaggacga
    12601 cgaaatggag ctggacacca tcggggacga aaagacggtg ctgtatctca ttatgagcga
    12661 tacggacacg acctttaatt tcattctggc tatgctccaa agccagctaa tcaacctgct
    12721 ttgtgaccgg gctgatgatg tgtacggcgg gcggctgcct gtccatgtac gcctgatttt
    12781 ggatgaattt gccaacattg gccaaatacc caatttcgat aaactcattg ccaccattcg
    12841 aagccgtgaa atatcggcct ctattatttt gcagagccag tcgcagctaa aagcgattta
    12901 caaggacgcc gccgagatca tttcggataa ctgcgatagt gtgcttttcc ttagtgggcg
    12961 cggcaaaaat gccaaggaga tttccgatgc gctggggaaa gaaacgattg acagtttcaa
    13021 caccagcgag aaccggggaa gccaaacgtc ccacggattg aactatcaaa aattaggaaa
    13081 ggagttgatg tcccaagacg aaattgccgt gatggacggc ggcaagtgta tcttgcagct
    13141 acggggtgtg cgcccgtttt tatcggagaa attcgatatt accaagcatc cccggtacaa
    13201 ataccttgcc gacgccgaca agaagaatac ctttgacgtg gacagatttt tgactatcac
    13261 gcgccggaaa cggcagcaag tggtaacgca ggatgaagta ttcgacctgt atgaaatcga
    13321 cctgtcggac gaggacgccg ccgctgaata aacagcggct tttttcattc caacaacaaa
    13381 atcaaaaaat cattatgagc cggaaaccgg cagaaagagg taaatttatg gcatttttta
    13441 attccgcagt tactactttg cagactatcg tgatcggact gggcggcgct ctgtgcgtat
    13501 ggggcggcat caatctgttg gagggatacg ggcaggacaa tccgggggca aaatcgcagg
    13561 gtgtcaagca gcttgtcgct ggcggcggcg tggccctgat cggtgtcact ctggttcccc
    13621 tgctgtccgg gctgctgggc tgatcctggc ctttgtccct gtccaaaccc ttaactaatc
    13681 gcctccgcgt cctcccggcc agaccgggag ggcgcgggaa aggaggtgct acccgtgatt
    13741 agtgaaatca ttgaagaatg gataaaaggc atcctcatgg acggtatcac gggcaacctg
    13801 tccgggctgt tcgatacggt aaatgccaaa gtcggagaaa tcgccgccga cgtgggacga
    13861 accccgcagg catggaacag cggtattttc aatatgctgc acagcctgtc ggaaaccgtg
    13921 atcgtcccca tagccggggc catactcgcc cttgtcatgt gctatgagct tatccagatg
    13981 ataacggaaa agaacaatat gcacgacttt gacagctcca tgttcttccg ctggatattc
    14041 aaatccgcgt ttgcaatcct gatcgtaacg aacacatgga acatcgtcat gggcgtattc
    14101 gacgccaccc aaagcgtcgt caatcaaagc gccggtgtga ttatcgggca gacatccatt
    14161 gattttaaca ccctgatacc agaccttgaa agccaattgg aggctatgag cgtgggcgct
    14221 cttttggggc tgtggttcca aacccttgtt gtgggcctca ccatgaatat cctttccatc
    14281 tgcattttcc ttgtgaccta tggccgcatg gtggaaatct atgttgtcac gtcgctgggg
    14341 cctatccccc ttgccacaat gggcaacgcg gaatggcgcg gcatgggcca agggtatctg
    14401 aaatccctgc tggcgctggg cttccaagcc tttttaatca tggttgtggt gggtatctac
    14461 gcggtgctga tacagaatat cggttccgct tccgacatat ccggcgcaat ctggggctgt
    14521 atgggctata cggtgctgct gtgcttctgc ctgttcaaga ccggcagtat atcaaaggcc
    14581 gtattcgggg cgcattgatc ccaaaatcca agcatacaca ggaggaactt gtttatttca
    14641 acgtacaaca tcatttacgc tgacccgccg tggcggtatg actgcaaaaa cgtacagggc
    14701 gcagcggaaa atcactattc caccatgagc attgacgagc tgtgcgccct gccggtggaa
    14761 cggctggcgt ccaaagactg cctgctgttt ttgtgggcca cattccccat gctgccggaa
    14821 gctctgcggt taatcaaagc gtggggcttt tcattcaaga ccgtggcttt tgtatggctg
    14881 aaacagaacc gtaaaagcct tacatggttt tacgggctgg gccgctggac aaggggaaac
    14941 gcggaaatct gcctgctggc aacacacggc aagccgaagc gtcgttctgc ctctgtccat
    15001 cagtttatta tcagcccgat tgaacaacac agcaaaaagc cggatgtgac ccgtgaaaaa
    15061 atcgtagagc tggcgggcga tttgccccgc gtggagctgt ttgccagaca gaaaaccccc
    15121 ggctgggacg tgtgggggaa cgaggtggaa tgtgacctta tcctgacgtg aaaggaggtg
    15181 ccaaatggct tatgtaaccg tccccaagga cttaacccat gtgaaaagca aggttgtgtt
    15241 tgggcttacc aaacggcaac tgatctgttt ttccggggcg ctggtgatcg gcgtcccctt
    15301 atattttctc acccgtgact atcttaccaa cagcgcggcg gccctgctca tggtatttgc
    15361 catgctgccg ggctttttgc ttgccatgta cgagcgtcac ggccagcccc ttgaagtggt
    15421 gattatgcag atcatccagt gctgcttcat ccagccaaag gagcgtccct atcagaccga
    15481 caacacctac accgcccttg tgcggcaata ccagatggaa caggaggtaa aggccattgt
    15541 ccaaaaagga aatcccaaga gaaaccgaaa aaacaaagct gacccgcgcc cagcgaaaag
    15601 aaattgacgc ggtaatccgt aaatataagg gcgacggcag gccccacacg gcgcagcaga
    15661 gcatccccta cgaggtcatg tacccggacg gcgtgtgccg ggtatcgccc ggcgtctttt
    15721 ccaagtgcat tgagtttgcg gacatcagct atcagcttgc ccagccggac acgcagacgg
    15781 ctatctttga aaaactgtgc gacctgtata actatgtgga cgcctccatt catatccagt
    15841 tttctttcct caatcggaaa gttgacccgg tgcagtacgc caagagcttt gagatcgcgc
    15901 cgcaggggga cgacttcgac gacatccggg ccgagtacac cggcattctg caaaagcagc
    15961 ttgcaaacgg caacaacggc atggtaaaga ccaaatacct gacattcacc atcgaggccg
    16021 aaagcgtgaa agcggcgcga gccagattga agcgcatcgg ctttgacctg ttaggctatt
    16081 tcaagagcat gggggccgta gcccatgtga tggacggctg ggaacggctg aacctgctgc
    16141 atggcgtgta ccacccggac ggcgagatat tcaattttga ctggaagtgg ctggccccgt
    16201 ccggcctctc caccaaggat tttattgccc cgtcctccct gtgcttcggc aatgcaaaga
    16261 cattcggcat gggcggcaaa tacggcgcgg taagtttcct gcaaatcctg tccccggagc
    16321 tgtcggacga tatgctggcc gacttcctca atacggaaag cggtgtttta gtcaatctcc
    16381 atgtgcaggc cattgaacag accaaggcga tcaagaccat caagcgcaaa attaccgacc
    16441 ttgacgctat gaagattgcg gagcagaaaa aggccgtccg cagcggttac gatatggaca
    16501 ttcttcccag cgaccttgcc acctacggcg aggacgccaa gaagctgctg acaaagttgc
    16561 agacccgcaa tgaacggctg ttccagttga cgttccttgt cctcaacgtg gccgacacca
    16621 agcaaaagct gaataacgac gtgttccaag ccgcaggcgt ggcccaaaag cataactgtc
    16681 cccttgtccg gctggactac cagcaggaac agggccttgc aagcagcctg ccgctgggcg
    16741 tcaatcagat caagatacag cgcagcctta cgacttccag cgtggccgtg ttcgtcccct
    16801 ttgtgacgca ggagcttttt cagggcggcg cggccatgta ttacggcatc aacgccaagt
    16861 cccgcaacat gattatgctg gacaggaagc aggcccgttg ccccaatgcc ttgaagctcg
    16921 gcacccccgg cagcggcaaa tccatgagct gtaaatccga gatcgtcagt gtgttcttaa
    16981 cgacgcctga cgatattttt atttcagacc cggaggccga atattacccc cttgtcaaac
    17041 ggctccacgg acaggtaatc cggctgtcgc ctaccagcaa ggactttgtg aacccgctgg
    17101 acatcaacct gaattattcg gaggacgata acccgctggc cctgaaatcc gactttgtgc
    17161 tgtcgttctg tgagcttgtc atgggcggca agaacgggct ggaagccatt gaaaagaccg
    17221 tgatagaccg ggctgtgcgc gtgatctacc ggccctatct ggcagacccc cgcccggaga
    17281 atatgccgat cctgtccgac ctccacaaag cgttgttaga tcagcatgtc ccggaggctg
    17341 accgggtggc gcaggccctt gacctgtatg tgtccggctc cctgaacgta tttaaccacc
    17401 gtaccaacgt ggacatcggc aaccggcttg tgtcctttga cattaaggag ctgggcaagc
    17461 agctcaaaaa gctggggatg ctcattgtcc aagaccagat atgggggcgc gtcacggcca
    17521 accgaagcca aggaaaggcc acatggtatt ttgccgacga gttccatttg cttttgaaag
    17581 aggaacagac ggcggcctat tctgcggaga tttggaagcg gtttaggaaa tggggcggca
    17641 ttccaacagg tgccacccag aacgtcaaag acctgctttc ctccccggaa attgaaaata
    17701 tccttgaaaa cagcgacttc attacgctgc tcaatcaggc gtccggggat agaaaaatcc
    17761 ttgcggaacg cttgaatttg tccaccgaac agcagaagta catcgacaat tccgaacccg
    17821 gcgagggcct tttgatcttt gaaaacgtgg tgctgccctt taccaacccc atcccgcaca
    17881 acacccagct ttacaagatt atgacgaccc gcttaaacga ggtggcggga gtatgacccg
    17941 gcgacgccac aaaggaggtg aaccaccatg aaagagctga aagccaaggc gaaagtgacg
    18001 caggccatga cccgcgacgg gctgacgctg gaaaatcagg ccaccggcga ggtggagaac
    18061 atttccagcc gggaggccga acaggatttg tccgcgccgg ggccgggcgg caccgccgat
    18121 aagctgctgg aacgggccga cgccatccat gacggccata aggccaagaa agccgcgaag
    18181 caggccgggg ctgtggccgc cgagggggac gctgccctgc accggccatc cgcccggctc
    18241 caactgtcgg aggaagaacg ggccgacccg gatttacagc cctatatccg aaaatcggac
    18301 aaacgggccg ataagctgga taaggcccgc gccgccatcc ccaagaagcg cgtcccggtc
    18361 aaggaaaccg tctttgacga ggcaagcggc aaagcaaaga ccgtcctgca ctttgacaaa
    18421 caggacaagg ggcctccctc cctgaaaccc aatccggcag gccgtcctgt ttccgaggtt
    18481 tttctattcg gccacgggaa aatccatgaa gtcgagcatg aaaacgtggg tgtggagggc
    18541 ggccataagg gggaggaact tgccgagcgg caggccggaa aagccatccg cagcaccatc
    18601 cggcaccaca ggctgaaacc ctactgggac gcggaaaagg ccgagcgcag gctattgtct
    18661 gccaacgcgg agtatttcta ccagaaatct ttgcgggata acccggagat cgcacaggcg
    18721 gccagcaacc ccatttcccg gttctggcag aaacagcaga tcaagcgcca gtacgccaag
    18781 gccgcccgga cagcgggcca gaccgcaggc gcggcggcac agggcgcggc caagacagcc
    18841 ggggcctcca cgaaagcggc caaggccgca gccaaagaaa gtgaacgggc ggcctccttt
    18901 gtggcccgcc actggaaagg ggcgctggcc gtccttgtgt tggggctttt gatgctgctt
    18961 atcatcggcg gcttacagtc ctgcaccgcc atgttcggca gcgccggaag cggcatcacg
    19021 gcgtcctctt acttttccga ggatagcgat atgttggccg ccgaggacgc ctacgcggag
    19081 ctggaagccg agctgcaaga ctatctggac aactacgagg ccacccatga ctatgacgaa
    19141 taccattttg accttgacga gatcagccat gacccctatg tgctgatttc cattctgtcg
    19201 gcgctccatg acgggatatt taccatcggg gaggtacagg gtgatttgtc catgctcttt
    19261 gacaagcaat atatcctgac agaaaacgtc gtggtagaaa cccgttaccg caccgaaacc
    19321 gatacatgga cggacgcgga gggcaacacc catacagaca cctaccaagt gccgtatgac
    19381 tattacatct gctacgtcac gctggaaaac ttcgacctgt cccatgtgcc agtctatatc
    19441 atggacgagg aaacgctaag tttctattcc ctgtatatgt ccacgctagg aaaccggcct
    19501 gatctgtttt ccggcagcca gtaccccaac gcctccaccc tgacgccgcc gacctactac
    19561 gacgtacccc cggaggcttt ggaggatgcc acctttgccg ccatgctcaa agaggcggaa
    19621 aaatatatcg gttatcccta tgtgtggggc ggcagctctc ccagcaccag ctttgattgc
    19681 agcggcttca tttcgtgggt aatcaatcat agtggatgga acgtgggacg gctgggggcg
    19741 cagggcctat ataacatctg cacccctgtt tccagttcgc aggccaagcc cggcgacctt
    19801 gtatttttca agggaaccta cgacaccccc ggcgtgtccc acgtcggcct gtatgtgggc
    19861 aacaatgtga tgctgcattg cgggaacccg atttcctaca cgaaccttac ttcaaactac
    19921 tggcaatccc atttttacag ttacgggcgt ttaccttaac gccagaaagg agctttcaat
    19981 tatggcaacc aaacttgacc gcatcgaaaa ggacatccag aaaaccaagt ctaagatcgc
    20041 ggagtttcag aaacagcttc gggagctgga aacgcagaaa accgaacagg agaatttgca
    20101 gatcatccag cttgtgcgcg gcatgaacat gaagccggag gaatttgccg cgttcttacg
    20161 cagcggcgca atgcaggccg cccctgccgc aaccccttac catgaacagg aggacaatgc
    20221 ccatgaagaa taagaccaga cggatttccc gtattctccc cgcacttctg gccgtgatgc
    20281 tctgcgtcac ggcctttccc gttaccgccc ttgcaggcgg caatgacccg gcaccggccc
    20341 cgctgccgga ggctacaacc gaacccgcta cgggcggttt ggagcctgaa acagacgaaa
    20401 ccggcaccgc cacggacggg acagaaaccg aggccgaaaa agataccggc cctctgaccg
    20461 gcaaggacat caccgacctg ttttccgcgc tgttcggttc caaggtgagc attgccgcca
    20521 cggacgacgg catccagatc accaccggca ccgacaaaaa agaacctgaa cagaccggca
    20581 ccgtcaccac caacggcggc aggctgaatg tccgcaccgg cgcggggctt gacaagaccg
    20641 cgttcaccca gcttcccaat gggacgacgg ttgaggtagt cggcacagac ggcgactgga
    20701 ttaaaattct cttgccggag cgcatcggct atgtccattc cgactatatg accgtcagcg
    20761 aaaaggaagt agcggctacc ggcgagggcg gtttttccct ctccattgac ccggaggaaa
    20821 ttgcttctct gctggaactg ttcaatggcg gcggggccag cgccgccctg tccccggacg
    20881 ggaacctgtc actgattgac gacatcggca gcgccgcgaa aaatggcaag cagtttatca
    20941 ccgtggaaac caaaaacggc aatgtgtttt atctgattat tgaccgggac gacgagggaa
    21001 aagaaactgt ccatttctta aatcaggtgg atgaagccga tctgctgacg ctgatggagg
    21061 acggggaaac cgtcaccccc gctgctgtct gctcctgtac cacgaaatgc aaggccgggg
    21121 ctgtgaacac gaattgcccg gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc
    21181 cgcaggaacc ccagccggag gaacccgaag ccccggagga agaacccaag ggcggcgcgg
    21241 gcggcctgat cgtgttcctg ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt
    21301 tcaagttcaa aaagcccaag gctgaaacca ccggcagcga cgatttgggc gagtatgatt
    21361 ttggggagga cgaggatttg gaggacgagg aacccgaaac cgagtttgac ccggaagctg
    21421 attttgtgag tgaacaggag gacgaggaat gacatataaa cttgtaatcg cggaaaaacc
    21481 cagcgtggcc cgcagcattg cgggcgtgat cggggccacg gagaaacacg acggctattt
    21541 ggaggggaac ggctatctgg tgagctggtg catcggacat ctggtgtcct ttgccgacgc
    21601 cggacgctat gacgagcgtt tcaaaaaatg gcgctatgag gatttgccca ttctcccgga
    21661 aacatggcag tacatcgtcc ccgacgacaa gaaacagcag tttgacgttc tctgttcgct
    21721 gatgcagcgc cccgacgtga ccggccttgt gtgcgccacc gacgccgggc gcgaggggga
    21781 attgattttc cgtttcgtct accagatggc gggatgccaa aagccctttg agcgcctttg
    21841 gatttcctct atggaggaca gtgctattaa ggacggcttt gcccacctga aaccgggcgg
    21901 cgactatgat ccgctgtatc aatcggccct gtgccgggcc aaggccgact ggctgatcgg
    21961 cattaacgcc acccggcttt tttccgtcct gtaccacaag aacctgactg tgggccgcgt
    22021 ccagacgccg accttaaaaa tgctggtgga cagggacgcc aagattaccg atttcaagaa
    22081 agagaaatat cacatcgtcc atatcacggc gggcggcgcg gacgcggtaa gcagccgctt
    22141 ttccgacgct gccgaggcaa acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg
    22201 cgtttccgtg acgcgggaga aaaagacgga acagccgccc aagctctatg accttaccac
    22261 cttgcagcgg gaggctaacc ggcttttcgg ctataccgcc aagcagaccc ttgactatgc
    22321 ccaggcactg tatctaaaga agctgctgac ctatccgaga acggatagtc aatatcttac
    22381 cgacgatatg ctgcccgccg tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt
    22441 tgccaagggc ctgaaccttt ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc
    22501 cgaccaccac gccattatcc ccaccgctga atttgtgaag cagggctttg gcactctggc
    22561 cgagagtgaa aaaaagctgc tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg
    22621 gccgcatatc tatgaggccg tcaccgccac atttacctgt gccgggcagg agtttaccgc
    22681 caagggcaag accgtccttt cccccggctg gaaagagatt gaacgccggt tccgttcttc
    22741 cttaaagacc gacgccgacg aggacgccga ggcggtgcgg gagctgccgg agctttgcga
    22801 gggccagacc tttgcagacg tggccgcctc tgtcaccgag catttcacca cgccgcccaa
    22861 gccctacacc gaagatacgc ttttgtccgc tatggaacgg gccggggccg aggatatgcc
    22921 ggaggatgcc gagcgcaagg gattaggcac accggccacc cgcgccgcca ttctggaaaa
    22981 gctggtgcag atgggctttg tccagagaaa aggcaagcag ctcattccca ccaaggacgg
    23041 catcaatctg gctgtggtgc tgccggaggc gcttacttct ccccagctta ctgccgaatg
    23101 ggaaaaccgg ctgaccgaga tcgccaaggg gcaggccgat cctgacgaat ttatggacgg
    23161 catcgagacg caggcgcggg agctggtgca gacctattcc tgtatcagtg aggaaaagca
    23221 gaaattgttt caggccgagc gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt
    23281 ctatgagggc aagaaaaact actactgcgg aaaccgggcc tgccagttcg tcatgtggaa
    23341 gaatgaccga ttttttgagg aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct
    23401 gctcaaaacc ggcaaagtca aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta
    23461 cgacgggacg gtgcttttgg ctgataccgg gggcaagtac gtcaactacc gcattgaaca
    23521 caggagctaa aggacagtaa cacagcaagc atagggagtg tggctacact cccggtaaaa
    23581 tcccccggcc gcgtccctgc cgggccgggg atttttgctt gttggggaag tccccaaccc
    23641 cctatttttt gaaacagaac agagaaagga gtgaccgcgt gaacgaagta ttttctatcc
    23701 aagtccataa ccggcagttt ttggaacagg gcaaacaagg cgtctggctg gatttgccca
    23761 ccacaaccga aacgctgcaa gccgccctgc gggatgttgg cattactgcc gacaatccgc
    23821 aggatttttt tatcaacggg tattcctgcc cggaggacag acaccttgcc attccctatg
    23881 acatggtgct ggccgccgac gtggacgagc tgaatttcct tgccgcccgg ctgggccagc
    23941 ttgacgccgc cgagatcgca gagctgaacg ccgccctgca aaatccccgt ggcggctttg
    24001 agaatatcgg ccagatcatc gactacaccg aaaatgtgga ttactatgtg catcttcccg
    24061 acgttagggg ggatggacag ttgggcgact actacctgaa ccgttccggc atggtggata
    24121 tgccggagga atggaaagcc gggattgata cggcgcagtt tggacgccac attgcccagc
    24181 aggaacaagg cgtttttacc cagtacggct atctggtgag aagcggcgac gaatggcagc
    24241 gcgtccatga gggccagccg gtgccggagg aataccgggt gatgcactac ccccagccgg
    24301 aagctgaccg ggaggaaccg caggcccagc agccggaggc atccgaaagc cccgacaaag
    24361 cgccgccccc ggttatcccg atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag
    24421 tgattactga ccgtctggaa accggcgtac aggagctttt tgaaagtagc cgctatatgg
    24481 cttacttatc cgctatgtcc aagatgcacg attacagtct gaacaatacg ctgctgatcg
    24541 ccatgcaggg cggttcgatt gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc
    24601 atgtgaagcg gggcgagaaa ggcattaaga tactggcccc ggccccgttc aaggttaagc
    24661 agcagatgga gaaaaccgac ccggccaccc agcagcccgt gatcggcgcg gacggcaagc
    24721 ccgtgaccga agaacaggaa gtgacggttc cggcctttaa ggtggtgaca gtctttgaca
    24781 tcagccagac cgagggcaag gattttccca tgccctatat cagtgaactt accggcgacg
    24841 tgcagcagta tcaggatttc tttgccgcgc tggaacggac atcaccctat cccataagca
    24901 ttgagccgat ttcagacggg gccaagggcc gctgcttcta cgcaaaacag cgcattgcca
    24961 tcaacgaggg catgagcgaa ttgcagaccc taaagaccgc catccatgag attgcccatg
    25021 ccaagctcca cgccattgac cgggaggccc ccgccgagga acaggcggcc cggccagacc
    25081 gcagcacccg cgaggtacag gccgaaagcg tggcctatac cgtctgccag tattacgggc
    25141 tggacacgtc ggactattct tttggctatg tggccgattg gagcagcggc agggagttat
    25201 ccgagctgaa agcctccctt gaaaccatcc gggccacggc cagcgaattg ataaccgaga
    25261 tcgacggcca ttttgcccag cttcagcagg agcgcgaggc ccggcaggaa caggaggccg
    25321 tcacggttga cgttaccccg gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc
    25381 cggcccttga tccggcagcc gagccgcttg tgactgtgct atggagcgaa agcccccagc
    25441 taaaggacgg ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg
    25501 cagcatatcc ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc
    25561 agggcgagcc ggacagctac gaggggcggc aggattttgg agatagggac ggttccctga
    25621 tcgaacatat ccagagctac caccaatttt acgcgcagga cgaaaactgg aaaaagcatg
    25681 taatcgctac aaccggcata gagggctggg agcaggacaa ggcccatcgg gatatgctgt
    25741 taggcgagtt tgtcccctat atgaagctgc attgcagcct tgcccaaatg gaacagacgg
    25801 cgcagggccg cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc tatttggggg
    25861 cgcttctctc ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag tatcagttgc
    25921 cggagccgcc gaaactggct gacttcgacc agagcttgca ggactacaaa gcacaggtac
    25981 aggccgagat cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg
    26041 ctggctatga agcaccccaa caggacacct tttccatcta ccagcttcga ggcggggagg
    26101 aaacccgcga ctatcggttt gagccatacg agcgtttaca ggccgcaggg ctggccgtgg
    26161 acagtgccaa ctatgacctt gtttatacgg caccgctgga taaaagcacg tctttggagg
    26221 atatttaccg cacattcaac atcgaccacc cggcagattt taggggacac agcctttccg
    26281 tttccgacgt ggtggtgctg caccagaatg gacaggacac cgcccactat gtagacagct
    26341 tcggctatat ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt
    26401 ctacggagca gaacgaaaac atgattgacg gcattatcaa caatacgccg cccgaaccgc
    26461 agccggagga acccgaacag ctatcttttg aggatgtggc agttgcttcc ggccagacac
    26521 aggaggaacc cccggaggct gcgcccccgc aaacggctgt ccgctattat cccatcaatg
    26581 agggagccgc ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg cccggcagcg
    26641 ccacggcgga ataccggcac tatgtcgatc aggccgtgga aattgcggag cgccagaaaa
    26701 agcgtgtgga gccagagtat cacgaaaaga tagacagcct ccttgacacc tacgcccgca
    26761 agctggccga gaacatgaat aagggctatg agattaccgc ccgcgtcccc tctgtcctga
    26821 ttgccgggcc gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc
    26881 gcaacatgga ggaatttcag tacattcagg gcttgcttga caaaatccgc agcaccggca
    26941 tgggtggtat cagcgccgac gatccgcagg ccgtttccaa gctggaaaag aaactggaaa
    27001 agctggaagc gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga
    27061 cgctggacgg atgcccccat ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg
    27121 cgtcgggctg gcactatgag aaaaaaccgt tccagtcctg gcagctctcc aacaataacg
    27181 ccgagatacg ccggttaaag ggccgcattg aggaattgac ccgccaaaag gaggcggcct
    27241 atgtgggatg ggagtttgac ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc
    27301 aaatcttctt tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt
    27361 tccgctggtc gcccaaggcc ggggcatggc agcggcagtt aaacgacacc acaatccgcg
    27421 tcgctgaccg tatcaagtgc atccagcccc tttccggcga aaagcccagc gccttacagc
    27481 ttgcggcccg cagggagaaa gaaaagccct ctatccgggc gcagctcaac gcggccaaga
    27541 ccgaacaggc caagcagcca ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa
    27601 tgcagaagtt tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg cggcagaaca
    27661 ccgcgtttta tcaaagtgac tttggcattg acaaagacat tatcaggcgg gatgccgcca
    27721 gcgatcaggc cgcagacaag accctgctct ggatgtcccg gccctccggg acgtactgtt
    27781 tccgggaacg tgatgttttc ctgaaagaca cccgacagta caacacatgg aaattttacg
    27841 gggagcagac ccgtgacaag atactggcct acgcggtgga gctgaccggc acacagggcg
    27901 gcactatccg gggcaatctc tatgagctgg actatccgca gcatttccgg cacgtcattg
    27961 aaaccgccca gccggtgagt gtgaaccggc tcttttatga gcatggcacc cgtgacatcc
    28021 cggagagcca gcgttttgac ggaagccctg accgggtatt gggaaacttt ttgtactatg
    28081 aggcccagcc ccatgacccg gctgtgctgc aggaggcgct acggacggag cagcacagcc
    28141 gggggcgggc cgtccccggc gatttcaaag tccacgtcac cgctttgcat gacagccgga
    28201 ttgaaaccga ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg cctaacagcc
    28261 ccaataaaac gcatttcatg gtggagatct ccccgcactt tgcggcgatt gcttccacca
    28321 aggataatga ccggctcttt gccatgctgc cctataaatc cctgtgtttt accgggatga
    28381 aaggcaggca tggcgtgtat gccgtgatta accgggacga gcgccgggat gtgagtattc
    28441 gcaggccccg gccctctatc cgcaagcagc tatccgacag caaacaggcc gccagcccaa
    28501 aaaaggcggc ggccagaacc aaaaagaatg aattggaggt ataaggaatg atgcacttta
    28561 ccgtggagga agaaaacctg atctgtatgt accacaatgc agaccggcgc agaacgataa
    28621 acaggctcac cgccgtcatg ccggttatgg atggggatat gcgggcgcag gcccagcaga
    28681 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
    28741 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
    28801 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
    28861 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
    28921 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
    28981 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
    29041 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
    29101 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
    29161 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
    29221 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
    29281 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
    29341 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
    29401 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
    29461 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
    29521 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
    29581 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
    29641 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
    29701 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
    29761 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
    29821 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
    29881 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
    29941 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
    30001 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
    30061 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
    30121 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
    30181 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
    30241 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
    30301 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
    30361 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
    30421 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
    30481 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
    30541 aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa
    30601 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
    30661 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
    30721 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
    30781 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
    30841 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
    30901 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
    30961 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
    31021 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
    31081 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
    31141 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
    31201 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
    31261 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
    31321 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
    31381 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
    31441 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
    31501 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
    31561 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
    31621 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
    31681 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
    31741 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
    31801 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
    31861 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
    31921 cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca gtcaatattt
    31981 attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc
    32041 gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca cctcccactt
    32101 acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt cttaaagttt
    32161 atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt accctgtgga
    32221 ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt gtgcccccac
    32281 gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag atggcgacga
    32341 tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg
    32401 cggttactga atagtataat gaaaccggcg cattttggat atggttttga agagagagaa
    32461 agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg ctccagaccg
    32521 ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg tatgaagaag
    32581 cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga agctgctgct
    32641 gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta tggtgaagtc
    32701 tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga ttgattttaa
    32761 taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg gcgattatta
    32821 catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag acttgctgat
    32881 tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg gcaagagcga
    32941 ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc
    33001 aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca aaacgagaac
    33061 gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga tccattgctt
    33121 gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa acattaagaa
    33181 ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag atacatacac
    33241 catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg
    33301 aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg atttttatgt
    33361 cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta agggcgtttc
    33421 tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca tacaccaagt
    33481 tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt atgaacgggc
    33541 atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg atgacagccc
    33601 tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa
    33661 agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg
    33721 cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat ttcacggtac
    33781 tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg
    33841 tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata tggggatatg
    33901 actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg gccaagctgc
    33961 cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa tcataataat
    34021 ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc tgctactgct
    34081 tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca gagtgatgga
    34141 attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga atagcgatag
    34201 gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct ttgagtttta
    34261 tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata catcaaatag
    34321 ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga ttgttgctca
    34381 aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa ttgttatgat
    34441 tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc aaatgacaac
    34501 gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag atgtaccgcc
    34561 gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt ccatgtatgt
    34621 caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac atgagttgga
    34681 ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc
    34741 tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag atcattctaa
    34801 gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt ccgaaatact
    34861 ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt tggacatagg
    34921 gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg
    34981 gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa
    35041 ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg
    35101 gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca
    35161 cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg atcaggcgcg
    35221 aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
    35281 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
    35341 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
    35401 t
//
LOCUS       DAYYHB010000021        34759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000021 DAYYHB010000000
VERSION     DAYYHB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.021"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..731)
                     /gene="gpmA"
                     /locus_tag="ACHA6B_000975"
     CDS             complement(45..731)
                     /gene="gpmA"
                     /locus_tag="ACHA6B_000975"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8948564.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(903..1484)
                     /locus_tag="ACHA6B_000976"
     CDS             complement(903..1484)
                     /locus_tag="ACHA6B_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8948565.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1617..2300)
                     /gene="rpiA"
                     /locus_tag="ACHA6B_000977"
     CDS             complement(1617..2300)
                     /gene="rpiA"
                     /locus_tag="ACHA6B_000977"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8948566.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2619..3032
                     /gene="rpsL"
                     /locus_tag="ACHA6B_000978"
     CDS             2619..3032
                     /gene="rpsL"
                     /locus_tag="ACHA6B_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8948567.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3116..3586
                     /gene="rpsG"
                     /locus_tag="ACHA6B_000979"
     CDS             3116..3586
                     /gene="rpsG"
                     /locus_tag="ACHA6B_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8948568.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3680..5767
                     /gene="fusA"
                     /locus_tag="ACHA6B_000980"
     CDS             3680..5767
                     /gene="fusA"
                     /locus_tag="ACHA6B_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8948569.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5939..7126
                     /gene="tuf"
                     /locus_tag="ACHA6B_000981"
     CDS             5939..7126
                     /gene="tuf"
                     /locus_tag="ACHA6B_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8948570.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7533..7841
                     /gene="rpsJ"
                     /locus_tag="ACHA6B_000982"
     CDS             7533..7841
                     /gene="rpsJ"
                     /locus_tag="ACHA6B_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8948571.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7872..8501
                     /gene="rplC"
                     /locus_tag="ACHA6B_000983"
     CDS             7872..8501
                     /gene="rplC"
                     /locus_tag="ACHA6B_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8948572.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8529..9152
                     /gene="rplD"
                     /locus_tag="ACHA6B_000984"
     CDS             8529..9152
                     /gene="rplD"
                     /locus_tag="ACHA6B_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8948573.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9152..9442
                     /gene="rplW"
                     /locus_tag="ACHA6B_000985"
     CDS             9152..9442
                     /gene="rplW"
                     /locus_tag="ACHA6B_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8948574.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9481..10314
                     /gene="rplB"
                     /locus_tag="ACHA6B_000986"
     CDS             9481..10314
                     /gene="rplB"
                     /locus_tag="ACHA6B_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8948575.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10357..10635
                     /gene="rpsS"
                     /locus_tag="ACHA6B_000987"
     CDS             10357..10635
                     /gene="rpsS"
                     /locus_tag="ACHA6B_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8948576.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10658..11005
                     /gene="rplV"
                     /locus_tag="ACHA6B_000988"
     CDS             10658..11005
                     /gene="rplV"
                     /locus_tag="ACHA6B_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8948577.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11019..11675
                     /gene="rpsC"
                     /locus_tag="ACHA6B_000989"
     CDS             11019..11675
                     /gene="rpsC"
                     /locus_tag="ACHA6B_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8948578.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11678..12112
                     /gene="rplP"
                     /locus_tag="ACHA6B_000990"
     CDS             11678..12112
                     /gene="rplP"
                     /locus_tag="ACHA6B_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8948579.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12102..12290
                     /gene="rpmC"
                     /locus_tag="ACHA6B_000991"
     CDS             12102..12290
                     /gene="rpmC"
                     /locus_tag="ACHA6B_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8948580.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12315..12581
                     /gene="rpsQ"
                     /locus_tag="ACHA6B_000992"
     CDS             12315..12581
                     /gene="rpsQ"
                     /locus_tag="ACHA6B_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8948581.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12639..13007
                     /gene="rplN"
                     /locus_tag="ACHA6B_000993"
     CDS             12639..13007
                     /gene="rplN"
                     /locus_tag="ACHA6B_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8948582.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13044..13352
                     /gene="rplX"
                     /locus_tag="ACHA6B_000994"
     CDS             13044..13352
                     /gene="rplX"
                     /locus_tag="ACHA6B_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8948583.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13380..13919
                     /gene="rplE"
                     /locus_tag="ACHA6B_000995"
     CDS             13380..13919
                     /gene="rplE"
                     /locus_tag="ACHA6B_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8948584.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13938..14123
                     /locus_tag="ACHA6B_000996"
     CDS             13938..14123
                     /locus_tag="ACHA6B_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8948585.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14159..14557
                     /gene="rpsH"
                     /locus_tag="ACHA6B_000997"
     CDS             14159..14557
                     /gene="rpsH"
                     /locus_tag="ACHA6B_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8948586.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14589..15125
                     /gene="rplF"
                     /locus_tag="ACHA6B_000998"
     CDS             14589..15125
                     /gene="rplF"
                     /locus_tag="ACHA6B_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8948587.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15286..15642
                     /gene="rplR"
                     /locus_tag="ACHA6B_000999"
     CDS             15286..15642
                     /gene="rplR"
                     /locus_tag="ACHA6B_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8948588.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15663..16163
                     /gene="rpsE"
                     /locus_tag="ACHA6B_001000"
     CDS             15663..16163
                     /gene="rpsE"
                     /locus_tag="ACHA6B_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8948589.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16178..16357
                     /gene="rpmD"
                     /locus_tag="ACHA6B_001001"
     CDS             16178..16357
                     /gene="rpmD"
                     /locus_tag="ACHA6B_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8948590.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16401..16841
                     /gene="rplO"
                     /locus_tag="ACHA6B_001002"
     CDS             16401..16841
                     /gene="rplO"
                     /locus_tag="ACHA6B_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8948591.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16841..18136
                     /gene="secY"
                     /locus_tag="ACHA6B_001003"
     CDS             16841..18136
                     /gene="secY"
                     /locus_tag="ACHA6B_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8948592.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18198..18845
                     /locus_tag="ACHA6B_001004"
     CDS             18198..18845
                     /locus_tag="ACHA6B_001004"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8948593.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19036..19254
                     /gene="infA"
                     /locus_tag="ACHA6B_001005"
     CDS             19036..19254
                     /gene="infA"
                     /locus_tag="ACHA6B_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8948594.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19287..19403
                     /gene="rpmJ"
                     /locus_tag="ACHA6B_001006"
     CDS             19287..19403
                     /gene="rpmJ"
                     /locus_tag="ACHA6B_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8948595.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19421..19786
                     /gene="rpsM"
                     /locus_tag="ACHA6B_001007"
     CDS             19421..19786
                     /gene="rpsM"
                     /locus_tag="ACHA6B_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8948596.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19814..20203
                     /gene="rpsK"
                     /locus_tag="ACHA6B_001008"
     CDS             19814..20203
                     /gene="rpsK"
                     /locus_tag="ACHA6B_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8948597.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20283..21221
                     /locus_tag="ACHA6B_001009"
     CDS             20283..21221
                     /locus_tag="ACHA6B_001009"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8948598.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21249..21629
                     /gene="rplQ"
                     /locus_tag="ACHA6B_001010"
     CDS             21249..21629
                     /gene="rplQ"
                     /locus_tag="ACHA6B_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8948599.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21901..22740
                     /locus_tag="ACHA6B_001011"
     CDS             21901..22740
                     /locus_tag="ACHA6B_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8948600.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22716..23585
                     /locus_tag="ACHA6B_001012"
     CDS             22716..23585
                     /locus_tag="ACHA6B_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8948601.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23575..24372
                     /locus_tag="ACHA6B_001013"
     CDS             23575..24372
                     /locus_tag="ACHA6B_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8948602.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24575..25321
                     /gene="truA"
                     /locus_tag="ACHA6B_001014"
     CDS             24575..25321
                     /gene="truA"
                     /locus_tag="ACHA6B_001014"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8948603.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25382..25588)
                     /locus_tag="ACHA6B_001015"
     CDS             complement(25382..25588)
                     /locus_tag="ACHA6B_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948604.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSSLNGVPEVASSKISRNFAKF"
     gene            complement(25654..28323)
                     /locus_tag="ACHA6B_001016"
     CDS             complement(25654..28323)
                     /locus_tag="ACHA6B_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8948605.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28561..28749)
                     /gene="rpmB"
                     /locus_tag="ACHA6B_001017"
     CDS             complement(28561..28749)
                     /gene="rpmB"
                     /locus_tag="ACHA6B_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8948606.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29019..29381
                     /locus_tag="ACHA6B_001018"
     CDS             29019..29381
                     /locus_tag="ACHA6B_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8948607.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29433..31115
                     /locus_tag="ACHA6B_001019"
     CDS             29433..31115
                     /locus_tag="ACHA6B_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8948608.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31232..33268
                     /gene="recG"
                     /locus_tag="ACHA6B_001020"
     CDS             31232..33268
                     /gene="recG"
                     /locus_tag="ACHA6B_001020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8948609.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33317..34318
                     /gene="plsX"
                     /locus_tag="ACHA6B_001021"
     CDS             33317..34318
                     /gene="plsX"
                     /locus_tag="ACHA6B_001021"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8948610.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34440..34682
                     /gene="acpP"
                     /locus_tag="ACHA6B_001022"
     CDS             34440..34682
                     /gene="acpP"
                     /locus_tag="ACHA6B_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8948611.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11339 a         6387 c         7474 g         9559 t
ORIGIN      
        1 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
       61 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
      121 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
      181 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
      241 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
      301 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
      361 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
      421 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
      481 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
      541 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
      601 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
      661 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
      721 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
      781 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
      841 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
      901 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
      961 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
     1021 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
     1081 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
     1141 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
     1201 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
     1261 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
     1321 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
     1381 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
     1441 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
     1501 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
     1561 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
     1621 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
     1681 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
     1741 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
     1801 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
     1861 aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc
     1921 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
     1981 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
     2041 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
     2101 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
     2161 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
     2221 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
     2281 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
     2341 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
     2401 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
     2461 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
     2521 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
     2581 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
     2641 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
     2701 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
     2761 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
     2821 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
     2881 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
     2941 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
     3001 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
     3061 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
     3121 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
     3181 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat
     3241 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
     3301 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
     3361 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
     3421 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
     3481 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
     3541 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
     3601 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
     3661 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
     3721 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
     3781 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
     3841 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
     3901 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
     3961 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
     4021 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
     4081 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
     4141 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
     4201 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
     4261 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
     4321 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
     4381 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
     4441 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
     4501 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
     4561 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
     4621 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
     4681 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
     4741 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
     4801 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
     4861 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
     4921 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
     4981 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
     5041 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
     5101 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
     5161 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
     5221 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
     5281 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
     5341 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
     5401 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
     5461 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
     5521 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
     5581 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
     5641 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
     5701 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
     5761 tgaataattt cttgaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
     5821 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
     5881 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
     5941 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
     6001 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
     6061 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
     6121 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
     6181 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
     6241 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
     6301 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
     6361 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
     6421 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
     6481 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
     6541 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
     6601 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
     6661 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
     6721 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
     6781 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
     6841 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
     6901 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
     6961 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
     7021 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
     7081 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
     7141 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt
     7201 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag
     7261 aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt
     7321 ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta
     7381 ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg
     7441 cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc
     7501 tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt
     7561 taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa
     7621 aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca
     7681 caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac
     7741 acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc
     7801 tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg
     7861 gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca
     7921 ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac
     7981 aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac caagatatgc
     8041 gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta
     8101 agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa
     8161 ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta
     8221 aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt
     8281 ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa
     8341 acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg
     8401 tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa
     8461 aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga
     8521 actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca
     8581 cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg
     8641 taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc
     8701 gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta
     8761 tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca
     8821 gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag
     8881 ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag
     8941 aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa
     9001 aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt
     9061 ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg
     9121 ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc
     9181 agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga
     9241 cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt
     9301 caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg
     9361 atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca
     9421 attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac
     9481 gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat
     9541 tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat
     9601 gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc
     9661 caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg
     9721 atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg
     9781 aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca
     9841 gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt
     9901 atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca
     9961 agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa
    10021 gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac
    10081 gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta
    10141 cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct
    10201 atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt
    10261 aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt
    10321 tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga
    10381 ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa
    10441 aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc
    10501 atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga
    10561 cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag
    10621 aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc
    10681 taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct
    10741 tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc
    10801 tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga
    10861 cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa
    10921 acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat
    10981 cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa
    11041 ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt
    11101 atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg
    11161 atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc
    11221 acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa
    11281 aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac
    11341 cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg
    11401 ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga
    11461 tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact
    11521 cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag
    11581 cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc
    11641 caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa
    11701 acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc
    11761 attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga
    11821 agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat
    11881 tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg
    11941 ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg
    12001 cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa
    12061 aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca
    12121 gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta
    12181 atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac
    12241 gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg
    12301 aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg
    12361 tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta
    12421 tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag
    12481 caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt
    12541 tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat
    12601 atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt
    12661 taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat
    12721 ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc
    12781 caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag
    12841 gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc
    12901 gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg
    12961 aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct tgagacactt
    13021 atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca
    13081 ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag
    13141 ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc
    13201 aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact
    13261 ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt
    13321 ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa
    13381 tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta
    13441 actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg
    13501 gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca
    13561 ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg
    13621 aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag
    13681 acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag
    13741 cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag
    13801 tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa
    13861 actctgatga agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat
    13921 taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa
    13981 aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc
    14041 gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg
    14101 gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat
    14161 ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa
    14221 acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa
    14281 acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg
    14341 tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc
    14401 taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt
    14461 aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa
    14521 aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt
    14581 gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta
    14641 ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt
    14701 cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca
    14761 gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag
    14821 gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat
    14881 tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac
    14941 cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca
    15001 aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata
    15061 aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta
    15121 aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag
    15181 tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt
    15241 tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca
    15301 gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact
    15361 gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt
    15421 gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt
    15481 ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa
    15541 aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa
    15601 gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat
    15661 tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc
    15721 gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg
    15781 gtgacaaaaa cggacacgta ggatttggta caggtaaagc tcaagaagtt cctgaagcaa
    15841 tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa
    15901 caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg
    15961 tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg
    16021 tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa
    16081 cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat
    16141 cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt
    16201 aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct
    16261 tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa
    16321 tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac
    16381 attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc
    16441 aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg
    16501 gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac
    16561 aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact
    16621 atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg
    16681 ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta
    16741 atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag
    16801 aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga
    16861 tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt
    16921 atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct
    16981 ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt
    17041 ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct
    17101 tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa
    17161 aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg
    17221 tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc
    17281 aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg
    17341 agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat
    17401 catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga
    17461 tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt
    17521 gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac
    17581 gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg
    17641 agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc
    17701 attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta
    17761 taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta
    17821 cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta
    17881 tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg
    17941 tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca
    18001 aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat
    18061 cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt
    18121 tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt
    18181 tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa
    18241 cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt
    18301 tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata
    18361 aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac
    18421 cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag
    18481 ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg
    18541 ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag
    18601 caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg
    18661 ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta
    18721 tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca
    18781 ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa
    18841 aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa
    18901 attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga
    18961 attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac
    19021 aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa
    19081 actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact
    19141 gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt
    19201 gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac
    19261 tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg
    19321 aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa
    19381 aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt
    19441 agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa
    19501 cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga
    19561 attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg
    19621 ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg
    19681 tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg
    19741 tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg
    19801 aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat
    19861 agaagcaggt attgcacata tccattctac attcaataac actatcatca tgatcactga
    19921 tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa
    19981 aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga
    20041 acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc
    20101 aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt
    20161 tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt
    20221 gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa
    20281 gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca
    20341 agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc
    20401 gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac
    20461 tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca
    20521 ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta
    20581 ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta
    20641 acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag
    20701 tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg
    20761 gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga
    20821 acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg
    20881 gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg
    20941 atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag
    21001 aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct
    21061 cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg
    21121 agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa
    21181 aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg
    21241 aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc
    21301 gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga
    21361 aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg
    21421 cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg
    21481 aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg
    21541 cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg
    21601 caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga
    21661 ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc
    21721 tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa
    21781 ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt
    21841 gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc
    21901 atggagccaa ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc
    21961 cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga
    22021 cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa
    22081 tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga
    22141 cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa
    22201 gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt
    22261 gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt
    22321 ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat
    22381 atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc
    22441 tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat
    22501 attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt
    22561 gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat
    22621 ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt
    22681 gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag
    22741 acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga
    22801 cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct
    22861 tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac
    22921 gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca
    22981 tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt
    23041 tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat
    23101 tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag
    23161 ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat
    23221 tggatgaacc aacggcagga cttgatcccc agggcagaaa agaaatgatg gagatgtttt
    23281 ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg
    23341 tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg
    23401 agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta
    23461 ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt
    23521 tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg
    23581 aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc
    23641 cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc aaataattgg
    23701 gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat
    23761 ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct
    23821 ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc
    23881 actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt
    23941 atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac
    24001 ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg
    24061 attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag
    24121 cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt
    24181 cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct
    24241 atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg
    24301 cataatagag acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt
    24361 ttaagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa
    24421 gaaaggatgc aagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca
    24481 ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa atcattattc
    24541 cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc
    24601 gtacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga
    24661 agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc
    24721 gggaaggaca gatgccggag tacatgcgat cgggcaagta atccattttg attatccaga
    24781 ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc
    24841 tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa
    24901 gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc
    24961 aagttatttt ccttacccac ttgatttgga aaaaattcag cgggcgcttc ctgatttatt
    25021 agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaaatacg
    25081 tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt
    25141 taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat
    25201 tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa
    25261 tgcagcagga cctacagcac accctgaagg actttatttg tatgaagtga aatatgaata
    25321 agaggttatg atagaagtgg tcagcttcaa gaaataagaa ggtatttacg aaaattactt
    25381 ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc tacttctgga acaccgttta
    25441 aggaagaggt cgtgagcctg tcatttaact ctgagtcaca aagagcaatt tcaaaaaaca
    25501 gcttctcatc tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct
    25561 ttttgttacc tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgcccttg
    25621 gtgcggacag tctgaacagg ttccatcgtt tctttatttt ctacgtcttc ttctgtgtgt
    25681 gtttcgtggt tgttcttcta ttgctggatc aatcgtgcca taaaaggggc gattgcggtc
    25741 aaggtaccag acataaagaa taaacaatgc aatacatagg acaaaaagga ttgcacctat
    25801 cgtaaatcct tcaggcaagt aactgaaagt gacagtatgt ttacctgctg gaacttttat
    25861 catcaagaat gcgtctttga aattgctgac cttgactggt ttcccatcaa ttttagcttt
    25921 ccatcctttg tcataaggaa tagttgtcac caacatggaa tcttcagcgg tatttacagt
    25981 cccagaagct tttctgccag tggcttccat ttcgacacct ttttctttga tctgattcat
    26041 tgcggcttga taggctacaa catctagacc aacgacttta ggctccatga aacttatttc
    26101 tttcgtccca taaaaacttg cagtaaatgt cactgttgtc tcttgaggat aatagccaag
    26161 atcatagtat tgaccagaaa tgttgatttg cgtttttcgc gatgtaccat tgacagtgat
    26221 cgtcgcacta gaactttcta attgcgcaaa attggttggg aacaaactta agtaagcttg
    26281 tgtatttgca ggcacatgga cttcccaagt gagctctttt ggctggttat ctgctgtttc
    26341 tgagtaagtg acgccagtcg ttgtctttgt gatcgtcgta ttcaactgat tcttcaaagt
    26401 aggtgtatag aatgtaaaat actgttcatt tagaccagac aatgtattga tcaagtttgt
    26461 ctgactggtc aaattatctg tcactggctg ctcaacatca ttaacggaca acggtgcagt
    26521 aaatgctagt ggcaatgcat tgacattctg atacagtgta tagtcgccgc tttgaccagc
    26581 ttggcggaag ccatatttac tgaacgtgct ttcgttttta gaaatgttat atttgattcc
    26641 tgtaaaccca tccatcagca aagtattatt gttataacgg atattcaaat ttgtccctct
    26701 tgaacggaaa cctaatttat ccaggtaaga ggaagaatta cgatttctta tagaagaaaa
    26761 caaactgata ccactgtaac catagttgat cgaatcgttt gcagatactg gatccagatt
    26821 ttccaagcgg taaaacgagt cggaatcttt ttttgtttca tttacaagag ctttcaagtc
    26881 aggataagga tcagtatata aactgcggga agcataattc caatcatcaa gaatgccatg
    26941 gatcatactg ttcgtattga ccagtgcttc agcagacatg atcaataata acaatatact
    27001 cagatgattg aatgaaagtt ttttatgctg ataaaatccc acagcaaaag cataaatccc
    27061 taagaaaacc actgtcaata caaaggaagt catcgtcaca taggtgtagc ttccttcagg
    27121 tttgatgccc catgccaccg caaagagagc agctacgacc aaaatgattc ctaacaacag
    27181 cccacgatcg tcttgttcta tcttttccca tccttttcct gcaagcagga gcactaagaa
    27241 agagaacaga taggcataac ggaataaaaa catattcggt gcatgcatac catgccagaa
    27301 caaattcaat ggaacaagat aaaaacttgc gataagtatg gcgaaaagac tgccgtacaa
    27361 aaatttattt ttcaacggta ctttccgact ggcaaaataa aacagacaga ggactaatgg
    27421 tagcagacct gcgtaaacga acggaatcga tccgtatttt gtcgtgtcat aaacaccaat
    27481 catatttttc atgattagat ccagataaga agtcgcttct gttttcaacg tagtgatctg
    27541 actcagtgcc tcgccatttg accgaagatc cagcactgct ggcaatacaa tgatcattga
    27601 agcgccgcct gccagtaaag aagtgatacc ataaggaaca atcgtttttt tatattctga
    27661 ccagtttgtc aatagacggg cgataaaata taagaaagaa aatacgccaa tcataaaacc
    27721 catataaaaa ctggataaaa acaagagtaa ataactgata aataaaagtt tcggccgttt
    27781 aagatccata acacgatgaa tccccaaaat cactaatgga agataaacaa atgcatccag
    27841 ccacatgatg atctccgaat gtgcagtgat aaatgagatc aatgcgtaac tgacgctaag
    27901 tgcaacatgc ccccagcgag gaatcttgaa cgtacgtgca agaaaccaaa aagccaatcc
    27961 ggcacagccg accttaagca aagtcagaac atacagtgca tctggcatca tctggtttgg
    28021 aaaaaagaac accagtggcg tgaagatccc gcctagatag taagaaatta aagataaata
    28081 attcaatcct agagaagcat tccatgtata aaaaatactt tgctttccaa gaagaacatt
    28141 tcggaagctg gcatgaaaat tagaaaactg tgaaaaggca tcacttgcca aaatacttct
    28201 gctgcttcca ggatagattc cgatacttag ataaacaagt gctaaaatca aaaacggaat
    28261 caggaaacta gctgctgcgt agcgtccgtt ttctctaaag aatgacttta aatttttttt
    28321 catatttatt ttccaccaaa tttctatgaa gttgtattca gtttatcata tccttgtttt
    28381 tctctgctta aaaagagtct agttttcaca aaaaaataaa atttagaata aataacttca
    28441 taatttgttc gtataacaaa aataaacgca aaaaaagggt aggaatcaaa agtcccaccc
    28501 ttaaaaattt ttattaaata aaaaaccgtt cactatctga acggtccaag tatcattcaa
    28561 ttaaacgcgt tcaacttttc ctgatttcaa agcacgagtt gacacccaaa cttttttagg
    28621 ttttccgtcg accataacgc gaactttttg taagttaggt ttaacagtac gttttgttga
    28681 gttcatagcg tgtgaacggt tgtttccact ttttgtcttg cgacctgtaa agtaacaaac
    28741 ttttgccatg gtaatttcct cctttgcctt gatttggagc aatagaataa ttcagctcat
    28801 accagactaa catatcatat agaatgggaa tttgcaagta cattttcata gatacaattg
    28861 acaattgaga agaataattg taaattaaac aaggttatac ctgaaatttt gcaaggtttt
    28921 tgagtacttt gggtaactgc cttttatttc aagttgcgct atgataaaat atgatagaag
    28981 aaaaatggga gttttttacc cgttaaggag gctcattcat ggctgtaaaa attcaaacgt
    29041 cagctggaac cattgaaatt accaatgatg taattgcaac agttgttggt ggcgccgcga
    29101 cagatgtgtt cggtgttgta ggaatggcca gcaaaaatca aattaaagat aatattactg
    29161 agattttgcg taaagaaaat tactcaagag gagtagttgt ccgccaagaa gataatggta
    29221 ttgctgtaga tgtttatacg attatcagct atggtacgaa aatctctgaa gtttcccgca
    29281 acgtccaaga aaaagtgaaa tacaatctag aggcattatt aggcgtaact gccaattcag
    29341 ttaatgtctt tgtacaaggt gttcgtgtac tgccagacta ggcaggagac tggctggtaa
    29401 ctcagcagtc gatctgaagg aggattatct aggtgagtgt aacagaaata agtgcaagcc
    29461 agttccagga aatggtccag gcaggtgcga atcgtctgca agtaaatgca gaatacgtca
    29521 attcattaaa tgtattccct gtgccagatg gcgatacggg aacaaatatg aatctatcta
    29581 tgactagtgg agctaaggca gtagtagatt caacctcaga aaaagtagga gaattagcag
    29641 caatgctttc aaaaggattg ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc
    29701 aattgtttcg tggattttct aagcaaattc cagatgttgt ctcattaaat gcttctgatc
    29761 ttgctgcagc cttcactcat ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag
    29821 aaggaacgat tttgacagta gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa
    29881 gtactgatga tgtcgttgaa gtaatgacag ctgttgtaaa aggagcaaag cgtgctttag
    29941 ctaaaactcc agacttgctt ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc
    30001 aaggactgct gtttgtttat gagggctttt taagtgcatt gaatggagaa ttccaagcgg
    30061 atgaaacata tgaaccttct ccagcagaaa tggatgatat ggtcaatgca gaacatcatc
    30121 gcagtgtcca aggtcaaatg gcgacagaag atatccattt tggttactgt acagaaatca
    30181 tggtaaaaat cggagaagga cctacagtcg atagtacatt cgattacgaa gaattccgta
    30241 actatttaga tggtatagga gattctttac ttgtggtcaa tgatgacgag atcatcaaag
    30301 tccacgtgca tacagaaaac ccaggagaag tcttgaatta tggacaaaaa ttcggttcat
    30361 tagtcaaaat caaagtcgac aacatgcgct tgcagcatga gacgattttg gaacacgatg
    30421 aagaagtgaa cgagtttgcg aaaaaagaaa taccacgtat cccgtatggt atcgtagcga
    30481 tcgctgctgg taaaggcgtc caagagctgt ttgaaagttt aggcgctaat tatgttatca
    30541 gtggtggaca aacaatgaat ccaagtacgg aagacattgt taaagcgatt caagaagtaa
    30601 acgcagataa agtgattgtc ttgccaaaca ataagaacat cttcatggca gctgatcaag
    30661 cagcagaagt agcagatatc ccagtagcag ttgttccttc aagaacgatt tcccaaggat
    30721 tgacagctat gctttcattc aatgatcaag cttctttaga agaaaacaaa gaaacgatga
    30781 cagaaatgtt gtctagcgtt gtgagcggtc aagtcactca cgcgatcaga gatacctcaa
    30841 ttgacggtgt agagatcaaa gaaggagact ttttgggcat gatcgatgga aaaatcctga
    30901 tctctaaccc agatatcaca gcaacagcgt taagtacact agaacaaatg atcaatgagg
    30961 acacagagat cgtaacgatc ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat
    31021 ttgctgagaa actaacagaa aaacatcctg atttggaaat cgagatccac caaggagatc
    31081 agcctgtcta tccttatcta ttttctgctg aataatttct gtgtcagaat atctttctaa
    31141 cacagtattc attcataaaa ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc
    31201 ctttttttgt taaaggaggg aggaaaaatt tatgcgttct ttagatgatc caatcactat
    31261 gctggcagga gtcggagaaa aaagagcagc tagtcttgcc agcttgggca tccaaacgat
    31321 tgaagactta ctgacttatt atccttttcg ctacgaagat attcaggaaa gaaatctaaa
    31381 cgagatccag gatcaagaaa aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat
    31441 gaatcgattt ggttataaaa aaagccgatt gcaatttcgt atgatgcaag aacatgatgt
    31501 gttcaacgtt tcttttttta atcaacctta tctaaaagaa aaagtcattt tatcggagga
    31561 aatcgccgta tacggtaaat gggatgcaaa gcgtaaagcc ttgaacggga tgaagatttt
    31621 aggttctcaa acaatggatg acttttcgcc gatatatcac gtcaacaaat caattcgtca
    31681 aacgacatta gttgatctaa tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga
    31741 aaatctgcca aacaaccttg tggagaaata tcgactgctt gaccgagctt ctgctgtaag
    31801 aagtatgcat tttccaaaag accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt
    31861 tgaagagttc tttttatttc aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga
    31921 aacgaatggt ttggagatcc tttatgatgt ccaaaaattg aaacaattta ctcaaaagct
    31981 accttttgaa ctgacaaatg cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg
    32041 cagtccgcag catatgcagc gacttttgca gggagatgta ggtagcggaa aaacagtggt
    32101 tgccgcgatt gcgttatacg ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc
    32161 tacagaaatc ttggcacaac aacatatgga aagcttgcaa cagctgtttg atcccaatga
    32221 agtaaccatc gcattgatta cggggtcaac gaaagcaaaa gaacgacgtg agctccttga
    32281 aaaactagag caaggggaaa ttaacgtcgt gatcggcaca catgcattga tccaagatgg
    32341 agtcgaattt cagaatttag gtctagttat tacggatgag cagcaccgtt ttggcgtgaa
    32401 ccagcggaaa gtacttcgtg aaaaaggctg gcgaccagat gtcttattta tgacagcgac
    32461 tccgattcct agaacattag ctattacagc atatggtgag atggatgtat ctatcattga
    32521 tgaacttcca gctggacgta tcccgatcga aacacgctgg gtaagaacac ctcaattgga
    32581 cagtgtactg gattggacga aaaaagaact ggcaagagga catcaaatgt atgtcatctg
    32641 tccgctgatt gaagaatcag aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa
    32701 acttcgtgaa ctatttgcac caaaatatga agttggcttg ttacatggta aaatgaagaa
    32761 tcaggaaaaa gaagtaatca tggaagcctt caaagacaac cagatgcaga tattagtttc
    32821 gactactgta attgaagtag gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga
    32881 tgcggatcga tttggattgg cacagctgca ccaacttcgt ggacgtgttg gacgcggaag
    32941 tgatgcttcc tactgtatct tagttgctaa tcctaaaaat gaactaggtg tggaacggat
    33001 gaaaatcatg acagagacca ccaatggttt tgtactaagc gaaaaagatc tggaattacg
    33061 tggtcctggt gaagtatttg gcttccgtca atctggtctg cctcaatttg cgattgctga
    33121 cttggtgacg gatggaaatg ttttggaggt agccagagaa gaggctgcag ccctttggca
    33181 aataaaggat tggcagatgc ttcctgagta tcagccttta gcgcaaagcc tccaaataga
    33241 aaatcaggat aatcgttatt tcgattagca gactgtgcta tactttatac tgatgacgga
    33301 aaaaggagtg gaaacaatga aaattgccgt agatgcaatg ggcggagatc acgcacctca
    33361 agcaattgta gaaggcgtgg ctttagcgca gcaagatttt ccagaagtag aattcttact
    33421 ttacggaaaa gaagcagaaa tcaagaaata cttaacaaat gaaaaaaaca tcacgattat
    33481 ccatacagac gaaaaaatca atagtgatga tgagccagta aaagcaattc gacgaaaaaa
    33541 gactgcatca atggtccttg ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt
    33601 ttcagcaggg aatacaggcg cgctactagc agcagggttg ttcatcgtag gacgtatcaa
    33661 aaatatcgaa cgccctggct tgatgtctac cttgccagta gtagggaaag aaggtgccgg
    33721 tttcgatatg ctggatcttg gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata
    33781 cggtatttta ggttcttttt atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc
    33841 actcttaaac aacggaacag aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga
    33901 actccttcag aatgaaacaa gcttgaattt tattggaaac gtagaagctc gtgagctact
    33961 caatggtgtg gctgatgtag tggtcacaga tggatttaca ggcaacgctg ttttgaaatc
    34021 tattgaagga acagcaatga atatgatgaa tctattaaaa tctgctattc taaatgaagg
    34081 gatcaaagga aaaatgggcg ctatgctttt aaaagacggc ttacgttctt tgaaagcaga
    34141 aatggattat tccaaacatg gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa
    34201 aactcatgga gcaacaggcc ccgatgctgt tcgctatacg atccgccaaa tccacacaat
    34261 gcttgagaca gatgtagttg gacaattagt cgaacaattt gaaaaaagcg aagaatagta
    34321 tttggatatc tgaagcgatc cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc
    34381 agtaaaatta gccatggtgt aggtaattga caagtgtatg attcttggag gtgaaaaagt
    34441 tgactcgcga agaagtattt aacaaagtag cgaaaattat ttcaaatcat tttgagatag
    34501 ataccgataa agtgacggat gaattgaaca tcaaagatga tttaaaagct gattcaatta
    34561 gtattatgga attcgtttta gagcttgaag atgaattcgg tacggaaatt tcagatgaag
    34621 acgctgaaca aattgaaaca gtcggtggcg cagtagatta tatcagcagc catcttaaat
    34681 aatttcaaat aaaagcaaag aaggataaaa agctgcatgc agctttttat ccttcttttt
    34741 ttaatctttt tgacacgta
//
LOCUS       DAYYHB010000022        34506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000022 DAYYHB010000000
VERSION     DAYYHB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.022"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA6B_001023"
     CDS             212..604
                     /locus_tag="ACHA6B_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8948612.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA6B_001024"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA6B_001024"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8948613.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA6B_001025"
     CDS             2698..2943
                     /locus_tag="ACHA6B_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948614.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA6B_001026"
     CDS             3165..4775
                     /locus_tag="ACHA6B_001026"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8948615.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA6B_001027"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA6B_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948616.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA6B_001028"
     CDS             5067..5936
                     /locus_tag="ACHA6B_001028"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8948617.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA6B_001029"
     CDS             6101..7372
                     /locus_tag="ACHA6B_001029"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8948618.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLITTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA6B_001030"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA6B_001030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8948619.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA6B_001031"
     CDS             8763..9026
                     /locus_tag="ACHA6B_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8948620.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA6B_001032"
     CDS             9471..9920
                     /locus_tag="ACHA6B_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8948621.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA6B_001033"
     CDS             9977..11395
                     /locus_tag="ACHA6B_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8948622.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA6B_001034"
     CDS             11395..12255
                     /locus_tag="ACHA6B_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8948623.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA6B_001035"
     CDS             12429..13202
                     /locus_tag="ACHA6B_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8948624.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA6B_001036"
     CDS             13349..13714
                     /locus_tag="ACHA6B_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8948625.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA6B_001037"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA6B_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8948626.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA6B_001038"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA6B_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948627.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA6B_001039"
     CDS             15560..16216
                     /locus_tag="ACHA6B_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8948628.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA6B_001040"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA6B_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8948629.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA6B_001041"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA6B_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8948630.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA6B_001042"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA6B_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8948631.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA6B_001043"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA6B_001043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8948632.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA6B_001044"
     CDS             21888..22202
                     /locus_tag="ACHA6B_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8948633.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA6B_001045"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA6B_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8948634.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA6B_001046"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA6B_001046"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8948635.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA6B_001047"
     CDS             23546..23875
                     /locus_tag="ACHA6B_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8948636.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA6B_001048"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA6B_001048"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8948637.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA6B_001049"
     CDS             24863..25687
                     /locus_tag="ACHA6B_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8948638.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA6B_001050"
     CDS             25680..26027
                     /locus_tag="ACHA6B_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8948639.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA6B_001051"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA6B_001051"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8948640.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28180
                     /gene="dinB"
                     /locus_tag="ACHA6B_001052"
     CDS             27077..28180
                     /gene="dinB"
                     /locus_tag="ACHA6B_001052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8948641.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPL"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA6B_001053"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA6B_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8948642.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA6B_001054"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA6B_001054"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8948643.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA6B_001055"
     CDS             31684..32451
                     /locus_tag="ACHA6B_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948644.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA6B_001056"
     CDS             32441..34483
                     /locus_tag="ACHA6B_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948645.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11091 a         6121 c         7247 g        10047 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat tactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt tgagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgccggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatga gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataat
//
LOCUS       DAYYHB010000023        34365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000023 DAYYHB010000000
VERSION     DAYYHB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.023"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..958
                     /locus_tag="ACHA6B_001057"
     CDS             269..958
                     /locus_tag="ACHA6B_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8948646.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            945..2153
                     /locus_tag="ACHA6B_001058"
     CDS             945..2153
                     /locus_tag="ACHA6B_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8948647.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2447..3718
                     /gene="serS"
                     /locus_tag="ACHA6B_001059"
     CDS             2447..3718
                     /gene="serS"
                     /locus_tag="ACHA6B_001059"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8948648.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4267..5751)
                     /gene="guaB"
                     /locus_tag="ACHA6B_001060"
     CDS             complement(4267..5751)
                     /gene="guaB"
                     /locus_tag="ACHA6B_001060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8948649.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6256..6951)
                     /locus_tag="ACHA6B_001061"
     CDS             complement(6256..6951)
                     /locus_tag="ACHA6B_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8948650.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7041..8141)
                     /gene="ychF"
                     /locus_tag="ACHA6B_001062"
     CDS             complement(7041..8141)
                     /gene="ychF"
                     /locus_tag="ACHA6B_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8948651.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8150..8341)
                     /locus_tag="ACHA6B_001063"
     CDS             complement(8150..8341)
                     /locus_tag="ACHA6B_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8948652.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8362..9252)
                     /locus_tag="ACHA6B_001064"
     CDS             complement(8362..9252)
                     /locus_tag="ACHA6B_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8948653.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9239..10006)
                     /locus_tag="ACHA6B_001065"
     CDS             complement(9239..10006)
                     /locus_tag="ACHA6B_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8948654.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10018..10731)
                     /gene="rsmG"
                     /locus_tag="ACHA6B_001066"
     CDS             complement(10018..10731)
                     /gene="rsmG"
                     /locus_tag="ACHA6B_001066"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8948655.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10963..12864)
                     /gene="mnmG"
                     /locus_tag="ACHA6B_001067"
     CDS             complement(10963..12864)
                     /gene="mnmG"
                     /locus_tag="ACHA6B_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8948656.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12881..14278)
                     /gene="mnmE"
                     /locus_tag="ACHA6B_001068"
     CDS             complement(12881..14278)
                     /gene="mnmE"
                     /locus_tag="ACHA6B_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8948657.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14577..14960
                     /locus_tag="ACHA6B_001069"
     CDS             14577..14960
                     /locus_tag="ACHA6B_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948658.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15128..15886)
                     /gene="jag"
                     /locus_tag="ACHA6B_001070"
     CDS             complement(15128..15886)
                     /gene="jag"
                     /locus_tag="ACHA6B_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8948659.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15904..16722)
                     /locus_tag="ACHA6B_001071"
     CDS             complement(15904..16722)
                     /locus_tag="ACHA6B_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8948660.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16719..17075)
                     /gene="rnpA"
                     /locus_tag="ACHA6B_001072"
     CDS             complement(16719..17075)
                     /gene="rnpA"
                     /locus_tag="ACHA6B_001072"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8948661.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17193..17327)
                     /gene="rpmH"
                     /locus_tag="ACHA6B_001073"
     CDS             complement(17193..17327)
                     /gene="rpmH"
                     /locus_tag="ACHA6B_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8948662.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17850..19184
                     /gene="dnaA"
                     /locus_tag="ACHA6B_001074"
     CDS             17850..19184
                     /gene="dnaA"
                     /locus_tag="ACHA6B_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8948663.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19382..20512
                     /gene="dnaN"
                     /locus_tag="ACHA6B_001075"
     CDS             19382..20512
                     /gene="dnaN"
                     /locus_tag="ACHA6B_001075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8948664.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20741..20983
                     /gene="yaaA"
                     /locus_tag="ACHA6B_001076"
     CDS             20741..20983
                     /gene="yaaA"
                     /locus_tag="ACHA6B_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8948665.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20970..22094
                     /gene="recF"
                     /locus_tag="ACHA6B_001077"
     CDS             20970..22094
                     /gene="recF"
                     /locus_tag="ACHA6B_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8948666.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22091..24037
                     /gene="gyrB"
                     /locus_tag="ACHA6B_001078"
     CDS             22091..24037
                     /gene="gyrB"
                     /locus_tag="ACHA6B_001078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8948667.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24057..26528
                     /gene="gyrA"
                     /locus_tag="ACHA6B_001079"
     CDS             24057..26528
                     /gene="gyrA"
                     /locus_tag="ACHA6B_001079"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8948668.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26764..27063
                     /gene="rpsF"
                     /locus_tag="ACHA6B_001080"
     CDS             26764..27063
                     /gene="rpsF"
                     /locus_tag="ACHA6B_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8948669.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27113..27625
                     /gene="ssb"
                     /locus_tag="ACHA6B_001081"
     CDS             27113..27625
                     /gene="ssb"
                     /locus_tag="ACHA6B_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8948670.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27651..27887
                     /gene="rpsR"
                     /locus_tag="ACHA6B_001082"
     CDS             27651..27887
                     /gene="rpsR"
                     /locus_tag="ACHA6B_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8948671.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28030..30003
                     /locus_tag="ACHA6B_001083"
     CDS             28030..30003
                     /locus_tag="ACHA6B_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8948672.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30009..30461
                     /gene="rplI"
                     /locus_tag="ACHA6B_001084"
     CDS             30009..30461
                     /gene="rplI"
                     /locus_tag="ACHA6B_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8948673.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30735..32102
                     /gene="dnaB"
                     /locus_tag="ACHA6B_001085"
     CDS             30735..32102
                     /gene="dnaB"
                     /locus_tag="ACHA6B_001085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8948674.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32882..34174
                     /locus_tag="ACHA6B_001086"
     CDS             32882..34174
                     /locus_tag="ACHA6B_001086"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8948675.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10480 a         6691 c         6815 g        10379 t
ORIGIN      
        1 catgtgaaag tcaataagaa aatgcttatt tatcaacatt tctagcttac atgatcattg
       61 gttagttctg gtcctttttc gttcgtcttt ataactccga gagacgtaaa atccttttgt
      121 tttcagttta atgttcatgt tcttgtctta ttcacaccaa ctagatgtct caccttctaa
      181 aattttcctt aacaaagtgc tgcttttatc tatttctgct tttttttgct agaataatag
      241 taacaatatg actctggagg atttatttat gaaaattttg attgctgacg atgataaaga
      301 aatcgttgaa ttattaagta tctatgttca caatgagggg tatgaagcag taaaggctta
      361 tgatgggaaa gaagcattat ctaaaatcag aacaatccct gatattgaat tacttatttt
      421 agatatcatg atgcctgaga tggatgggat gcaagtagtg aaagagctac gaaaagaatc
      481 ccaaatacca atcattatgc tgacagcaaa aactactgat atggataaga tcaaaggact
      541 ggtcgctggt gcagatgatt atgtgaccaa gccatttaat ccgttagaag taatggctag
      601 agtaaagtct ttattacggc gtacaaaaat gcagattgcc ccagatcagc ctgatgtatt
      661 agaagtaaat gcattaatca tcaataaaga ctctcatgaa gttaaaacaa tcgatgggaa
      721 agaaatccag ttaacagctt tggaattcgg tatcctctac ttgcttgcta gtcatcctaa
      781 tcgagtattt agcgcggatg aaatttttga gcgtgtttgg aaacaagaaa gtattgtctc
      841 tgctaaaact gtcatggttc acgttagtca tttaagagat aagattgaag aagcaactgg
      901 tggtgaaaaa gtcattcaaa ccgtttgggg agtgggctat aaaattgacg caagatagta
      961 aacgagcaac tgaaaaacgc cgtcggatca atttgacttc aaaagaaatc agcgaattgt
     1021 tagcggaagg aatcatcacg attatccttt tgcttctatt aaatgtttct attctggttg
     1081 ttatcagttc ggtgatcaac agttcacctt ctttgacgaa tgcgatttgg gattcaaaga
     1141 atatttttgc agaacgattg aacactgatt tgttctggaa cggccggaat tttattattc
     1201 cctttttctt tctattagat attggtgttt tgtattggcg cttgattcga agatatcgcc
     1261 aaatgcaatt gcggcatatc atcagcgaac ttcactacat cgcaaacgga aattatgatc
     1321 atcgtatccc ttttgaactg agtggtgact taagccgagt ggtaacaagt atcaatggat
     1381 tggtggatag taccgtagca gcgattgaag acgaacgtaa aatcgaaaaa tcaaaagatg
     1441 aattgatcac gaatgtcagt catgatatcc gtaccccact cacgtccatc attggatacc
     1501 taggactgat agaagatggc cagtatcact cagaagaaga tcttttaaag tatacgcata
     1561 ctgcttatat caaagcaaag cagatgaaat ctttagtgga cgacttattt gaatacacta
     1621 aggtacgcca gcctgctgtc ccggtgaatt tttctgcctt tgacatgatc cagttgatcg
     1681 aacagttagc agctgatttt gagttggaag cttctaaaaa aaatatccaa atacttgttc
     1741 aatcaaaagt agactccctt atcatggatg gcgataccga aaaacttgta cgtgtcttca
     1801 ataatctttt gacgaatgcg ttgaaatacg gaaaaggagc aacgaagatc gtaatcgaag
     1861 tagaacgtat cggttcagaa gttgttgcaa cagtcaaaaa taacggagca atgatcccac
     1921 aacaggcaat cgataatcta ttcgaccgtt tttatcgtgt agaagaatct cgttcacaag
     1981 caactggagg aactggactt ggtttagcaa tcgctcaaag cattgtggct ttgcacggcg
     2041 gatacattta tgcaaaatca gataagcaat ggacttcttt catcatgcac cttccaatca
     2101 agaaaaatga aaaattacct attgatacac aggaagttat tgacgaaagc tgaaatattc
     2161 gctattctta ttacaattat tagaataaat gaaaaacaca gaacccagta cactttattc
     2221 tttttcagag agctgatggc aggtgtaaat cagcaaagga taatttggaa gttccagttc
     2281 gtttacgtgc cctttcgaaa taagaaaggt cggttcaaca gccgttatgt gttcaacaag
     2341 tgcagctgac gctgaacttg ggtggtaccg cgaatttcag actgattcgt cccaagaata
     2401 atgggatgga tcagtctttt tttattttca aaaaggagga aatcatatgt tagatgtaaa
     2461 aatgatgcga caaaatattg aagaagtaca agaaaaattg atgactcggg gcgtaaaaaa
     2521 agaaacatta gctgaattca tggaactaga tgaaaatcgt cgtaagctac tagtcaaaag
     2581 tgaagaatta aaaaaatatc gaaatgatgt ttctgctgag atcgcccaat tgaagcgtgc
     2641 aaaagaagat gcttctgcta aaatcgctga aatgaaagaa gtcggtggca acatcaaagc
     2701 attggatact gaaattgctg aagtggatga aaaattgaaa caaattgcaa ctactttgcc
     2761 aaaccttccc catgattctg tgccagttgg aacagatgaa gaggacaatg tagaaatccg
     2821 tcgttggagc gaaccacgta catttgcttt tgaaccaaaa ccacactggg aaattgcaga
     2881 aaatcttgat attttagatt ttgaacgtgg cgcaaaagta gccggtagcc gttttgtcta
     2941 ttacaaagga ttaggtgctc gattagaacg tgctgtctac aacttcatgc ttgatcagca
     3001 tatttacgaa cacggctata ctgaaatgat cactccttat atcgtcaaca gtaaagcgat
     3061 gtttggtacg ggacaatttc caaaattcaa agaagatgtt ttccaattgg agaatactga
     3121 tctaacatta atcccaacag ctgaagtacc attaactaac tattataatg atgaaatttt
     3181 agatggtgca gatttaccta tttatttcac tgcactaagc cctgcttttc gttctgaagc
     3241 cggaagtgct ggtcgggata cacgaggact gattcgtctt caccaattca ataaagtaga
     3301 aatggtcaaa ttttcggatg tagagcattc ttacgaagaa ttggaaaaaa tgacagctga
     3361 tgcagaagat atcttgaaaa aattgcattt gccttatcgt gtgatggcct tatcaactgg
     3421 agacatgggc ttttctgcag ctaaaactta tgacttagaa gtatggatac ctgcacaaga
     3481 tacttatcgt gaaatcagtt cttgttctaa ctgtgaagat ttccaagcac gccgcgcaat
     3541 gatccgttat cgcgataatg atggaaaaat ccactatact catactctga atggttcagg
     3601 acttgcagtt ggccgtacag tagctgccat tttggaaaac taccaaaatg aagatggttc
     3661 tgtcacagtt ccagaagcgc tcgttcctta tatggggaat ctgaaagtta ttaaataaag
     3721 aggctgtgaa aaagccgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     3781 cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca ccatttatcg
     3841 agaggttgtg aaatcagcga attaacctga gtattagaga aaaaaatgga aaaatagtcc
     3901 ctcatatttt accgtttgtt gaactaatac cgagggtcag ctgattgaac accgtttagt
     3961 ggagaggtcg taagcctacc ccccagctcc tagttacaag gacaaattca aaaaacagct
     4021 cttcatattt tatgaatgtt gtacttgtga ttcggaggtg gcaagggaac actgtttatt
     4081 caaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
     4141 tgcttttcaa atttttgtga atttcttctt atttccgaag gagttgcttc tacttccgcc
     4201 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta
     4261 atgtttttaa gattcaactg aatagtttgg tgcttctttt gtgatttgta catcatgagg
     4321 gtgtgattct tttaatccac tgccgctcat ttggataaat tgcgcttcat cacgtaattg
     4381 ctgtagattg gctgccccaa cataacccat tcctgaacgc aaaccgccga tcatttggaa
     4441 aataatatct gcaacacttc ctttataagc aacccgacct tcgattcctt ctggtactaa
     4501 tttatttgct tcattgacac taccttggaa gtaacggtct tttgaacctt tttccatcgc
     4561 gcctaatgag cccatacctc ggtatgtttt gaatcgacgt ccttggaaga tttcgaattc
     4621 acctggtgat tcatctgtcc ctgccaacat acttcctagc atcactgcat gtccgccggc
     4681 agctaatgct ttgacgatgt caccagaata tttgatccct ccgtctgcaa taatcgcttt
     4741 gccatattga cgagcaactg atgctgcatc ataaatagct gtgagctgag gtacacctac
     4801 accggctacc acacgtgtcg tacaaatcga tcctggtccg ataccaactt ttacgacatc
     4861 tacacctgca tcatataaag cttttgtcgc ttcagccgtc gcaacgtttc ctgcaatcaa
     4921 tgttgcatct gcaaaagttg aacggatttc ttgaattttt cggatcacac ctgcactatg
     4981 tccatgagct gtatcgatga tgattgcatc tgcccctgct tctaataaag cattcgcacg
     5041 ctcaaatgta tcgcttgtta caccaacggc tgctgctaca agcaatcgac catgttcatc
     5101 ttttgctgca tttggaaatt cgatcacttt ctcgatgtct ttgatcgtga tcaatccgct
     5161 taaacgacct tcgttatcta cgattggtaa tttttcgatt ttatgttttt gcaaaatctt
     5221 ttctgcatct tttagagagg tacctactgg ggcagtcact aagttgtctt ttgtcatcac
     5281 atcagcaatc ggcatgtgat aatctgttac aaaacgcatg tcgcggtttg taattatccc
     5341 taccaatttt ctgttttcaa gtgtttcaac gattggcaca ccactgatac gatatttgct
     5401 catcaattct tctgcgtctg ctacaagatg ctgtggcgtt aagaagaacg gatcaatgat
     5461 tacgccactt tcagaacgtt tgacttttct tacttcatcc gcttgctggc tgatcgtcat
     5521 gtttttatgg atcacaccta gtccgccttg tcttgccata gcaatggcca tcttgctgtc
     5581 ggtaacagta tccatacttg cactgatgat tggaatattc aacttaatat ttttagctaa
     5641 ttgtacgctc atatccactt cgtttggtaa cacgtgactt tctgctggta tcaataagac
     5701 atcatcaaaa gtaaatcctt ttttcgtgaa ctttgtttcc cagttggaca tttttagtac
     5761 cactcctcta atttagttta tctattaaaa taattgattt tttcatttaa gtcaatgatc
     5821 tacttggaat caagttagct ttctcataga ataccaatat aaagcgttta catctgtgtg
     5881 tattaaaaga gattgtaaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     5941 acagcttctc atgtttttga attggttggg tttattaccg cagatgcaaa cttttgaaca
     6001 ccgtttagtg gagaggctgt gaaaaagctg cttagtttcg agtagtaaga aaaattttgt
     6061 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     6121 acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc ttactattat
     6181 ttaactatgg ataaacaaaa tgaggttctt tactacccct agatatctag caaaatagct
     6241 gatctttttg ctagtttatc ttcttgggac ggataaactt ccaataatcc cgtatttttt
     6301 cttcaagtag taacggattg ctaaagcagt tgccccgata atgataacta ctactggatc
     6361 taatactgga ttgataactg gtggcaatag tgttgtcgca gaaaacagta cgacccaaag
     6421 aagtgtcgtt ccacctagga taagcaatgt cttacccatt cctggtttct tgcttcgatc
     6481 tgctccggga tattcatatt gatagatgta tttgtacatc atataaaaga cccagccacc
     6541 gattgcagaa cctagaacga gtgtagaaaa gccataagct ggcgttgttc cacgtgaaaa
     6601 caatccggta atcgcaatca tgatacttaa tagaccaagc aacagcaacg tattatctaa
     6661 ccacatcaat aaaggggtgg atgaattttt tacttctgct ggcttattga tgattgcttc
     6721 cgttctttca gagactgtcc caaacaattg acgggctgtt cttccgcctt tttgttctgc
     6781 aactaaagta ggcagaatgt catgaagggc ttttactttt tcttcttcag acaaattcgc
     6841 tgcttctaaa gattttttca aatcaaaaat gtactgttga tttcttttgg ttaattttgt
     6901 ttctaaacta ttgttttctt ttacgattgc tcgtaattgt tctggttcca ttatctaata
     6961 gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa agacattcta ccatattcta
     7021 cagctagttt gatagccctt ttacacatta aagcggaata acattacgtc gccgtcttgt
     7081 acgacataat cttttccttc taatcgaaca cgtccagctt cttttgctgc atgcatgttt
     7141 ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac ggataaatcc gcgttcgaaa
     7201 tctgaatgga tgattcctgc tgcttgtggt gctttgattc cttttttgaa tgtccaagca
     7261 cgtacttctt gttccccagc agtaaagtaa gtagctaagc ctaacaaatc ataagcagca
     7321 cggatcaact gatccaaacc tgattcttcg attcctaaag cttctaaaaa ttccgctttg
     7381 tcttcttcat ctaattctgc gatttcttct tctgcacgag cactgaccac gatcacttct
     7441 gcattttctt ctgcagcaaa gttgcgcact tcttgtacat aatgattcgc gtctgcatct
     7501 gctacgtcat cttctgagac attggctaca taaagaattg gttttgtcgt caataaaaat
     7561 agtgatttaa cgattttttg ttcttcttca gtaaattcga tcgttcgggc agattttcct
     7621 tcttccagta caggtttgat tttatccagt acagctagtt ctgccactgc atctttatct
     7681 ttggttttag caacttttgc tacacgagta tagcgtttgt tgatagattc taagtctgct
     7741 aacactagtt ccaagttgat cgtatcaata tcttctagtg gatcaacacg tccttctacg
     7801 tgagtaatat tatcatcatc aaaacagcga acaacgtgac aaatagcgtc tacttggcgg
     7861 atatggctta agaattgatt tcccaaacct tctcctttac ttgcgccttt tacgattcca
     7921 gcaatatctg taaattcaaa agtcgtcgga acagtttttt tcggatgtac aagttctgtt
     7981 aagcgttgca aacgccagtc aggtacttct accatcccta cgttggggtc gatcgtcgca
     8041 aagggatagt ttgctgcttc tgctcctgct tttgtgatcg cattaaacaa agttgatttt
     8101 cctacgttgg gcaacccaac aattccagca gtcaatgcca taaatttatt cactctttct
     8161 ctgcattttt ttcaattact ttttttaatt tcttttcaaa atctcgacgg gtcatcatga
     8221 cgatatgtcc gcaattcata catctgattt tgatatctgc gcccattcga atgacttccc
     8281 agcgattcgt ctggcaagcg tgaggttttt tcatttcaac gatatcgtgc aagtcgtaca
     8341 taaaaatagc ctcctttttg tttattcttc atcaaattga atattcaaga tatcaagaat
     8401 tcgagtcaaa tctttttgag aaagatattc aatctcgatc ttacctttcc catttttttc
     8461 ttggatagct acacttgtac caaatttatc catcaaacga tcttcgcttt ctcggatata
     8521 atatggtttt tcctttgcta gccttgggat ttttttcttt tcttttccat cattttgatt
     8581 taactcagaa acaagctgtt ccagttggcg gacagttaaa ttttcagtta ctgcacgatt
     8641 ggctaatttc aagatcaatg ttttattttt taaacctaac agcgtacggg cttgtcccat
     8701 agataaacgc tgatcttgta ccatttcctt gacaagatcc ggtaaagaaa gcaagcgcaa
     8761 ataattggcg atataaggtc tgctctttcc taaacgacta gctacttctg cttgtgttag
     8821 tttcaggttt ttcatcagca tctcatatgc ttctgcttct tctaacggat tcagatcttc
     8881 tcgctgcaag ttttccagca ctgctacttg catcatcgct tcttcgtcaa agtctctaac
     8941 gatagcaggg atcgtttctt tttcagctag tttggacgct cgaaaacgtc gctcccctgc
     9001 aataatctca tatcctttta ctgcggaacg gcgtacgatg atgggttgaa agacacctga
     9061 ttgttcaata gaattagcta gttcctgcaa agatacttct tcaaatgttt tacggggctg
     9121 atacggattt ggtcgcagct cattcaatgg tagctggaca acggtatcat cttttacatc
     9181 caccgtctct aagttttcta aatcttgaaa cagtgcatcg attcctcggc ctaatccttt
     9241 acttttcttc acgacttacc acttcctttg ccaatgcttg gtagacttca gctcctcgag
     9301 aacgtggatc gtagtcgatg atcggttttc catgactagg tgcttctgat aaacgaatat
     9361 ttcttggaat gatcgtttcg taaacttttt cgcggaagta tttacgtact tcttctacga
     9421 cttcatttcc cagatttgtt cgtgcatcat acattgtcag taagactccc tcgatctcta
     9481 attctggatt aaagtgtttt tggactaaac gaaccgtatt cagtaattgg cttaatcctt
     9541 ccaatgcgta atactcacac tgaacaggta tcaaaatcga atcacttgct gtaaatgaat
     9601 tgattgtcag atggccaaga gaaggcgggc aatcgatcaa aatataatca tactgactgc
     9661 ttacttctgc taaagatcct tttaatcttg attctcttgc catcattgac gtcaattcga
     9721 tctctgctcc tgctaattgg agagtcgcag gcacaatact caaattttcg tgttcagtga
     9781 tcaaagttgc ctcatcaatt ggcaattcat tgactagaac atcatagatg tctcttgtca
     9841 catcaggttt tctgatcccg acaccactgg tagcatttcc ttgtgcatcc atatccacta
     9901 gcaatacttt tttgccaagg ctagcaagac aagcacctag attcaccgtc gtcgttgttt
     9961 tgccgacgcc acctttttgg ttcgcaacag atattatttg tgccatctct tctcctccta
    10021 tttgattggc tgcttattag gcagtccggg ctttcttggg tatttctttg gggttggttt
    10081 ctttttttga ataacgatga tatgccgttg atcagagaca ttcggcaagg taaccgcctc
    10141 ttctttgagg aatttaccac ctaataaagc aatcgcttgt tttgcttctg tcaattcctc
    10201 ttcacttttt gctgctttca atgcataaaa atagccttct tgtttagcca agggcaaaca
    10261 caattccgct aacacattca aacgagcaac cgcacgagca gtgacaaggt caaatgatgc
    10321 acgaaagtga ggattttgcc caaatgtttc tgcccggtca tgatataagg aagcatcgat
    10381 tcctaataat cgggttaact cagataagaa ggttattcgt ttattcagag aatcaacgat
    10441 cgttatctgc agttctggaa aggcaatttt taaaggtatg ctaggaaacc ctgcccccga
    10501 accaacatcg cataaagata aaggttgcgt aaagtctact tcaaatgcta atgtaagtga
    10561 atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta atagctgtaa ggttcatctt
    10621 ttcgttccat tcgactaatg tccgataata aatagcgaat tgattcattt gatgatcggt
    10681 aagctctatc cctttttctg ccaaggattg gcgaaattct tctggtatca taaatattcc
    10741 tttcattgtg aaacagcaat tgtttcacag attctctccc tactttaacg caaaatctct
    10801 agacatgcaa tccttccata tggattctta aaaatttgaa aagtaagaaa agccgataga
    10861 ctttttcttt ctagtatgac tcataaaata tacacagaag agagaactag ctttgagcaa
    10921 tttctgcaaa aactagttct ctcttctatt tattattacg tattacgctg gttcactgac
    10981 tttcgcaatc ttcccttgtt cgatatagac cattaggata cttaggtctg cagggtttac
    11041 gccactgatt cggctagctt gggcaattgt ttctggttgg attttctgca atttttgttt
    11101 tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg ttttctggaa tctttttcgc
    11161 ttccatgcgt tttaattttt ctactttttc catggctttt ttgatgtagc cttcatattt
    11221 gatttggatt tctacttgtt cgatttcttt tcgtgtcaat tcttcatttt ctggaataaa
    11281 ccgtagaagt tctgcataag taatctcagg acgtcttaaa aagtcgcttg ctagtacacc
    11341 atcttttaat ggtgccgcat taatcgttgc taaaaagtct tggacttctt ctgttggttt
    11401 gattcgtaca ttcatcaaac gtttgatttc ttgttcaact gcttcttttt tcttcaaata
    11461 attcttgtat tgatcttctt ttactaagcc tacacggtga cccatttctg ttaatcgcaa
    11521 atctgcatta tcatgacgta aaagcagacg gtattcagca cgtgaagtca gcaaacgata
    11581 aggttcgttc gttccttttg ttaccaaatc atcgatcatg acaccgatgt aaccgtcact
    11641 tcgtttgatc actaaaggtt cttttccctg aactttcaaa gctgcattga taccggcaat
    11701 cagcccttgt ccagccgctt cttcataacc gcttgtacca tttgtttgtc cagctgtata
    11761 aagattttca acgactttag tttctaatgt cggacggagc tggtgtggct ctactacgtc
    11821 atattcgata gcatatcctg tacgcatcat ttctactttt tctaatccag caatagaatg
    11881 aagcatgttg acttgaacat cctctggtaa agaagtagat aaaccttgaa catagacttc
    11941 ttccgtattt aagccttctg gttccaaaaa taattgatga cgtggtttgt ctgcaaaacg
    12001 cacgatctta tcttcgatgg atggacaata gcgagcgcct accccttcaa cgatccctgt
    12061 aaacattggg gcacgatgta agttgtctcg aataatttgg tgagtcgtct cacctgtata
    12121 ggtcagccag caagattcct gatctagttt ataggcacta tctggtgtgc tgaagctaaa
    12181 atgattcggc tctttgtcac caggctgctc ttctgtaaca gaatagtcga tcgtacttga
    12241 ttttactcga ggcggtgttc ctgttttgaa ccgttcgata gcaaagccaa gttcttttag
    12301 atgattcgct agtccaatag aaggttgaga gttattcgga cctgatgaat atttcaattc
    12361 accgataatg atttctccgc gaagtgctgt tccggctgta ataacaactg ctttggcccg
    12421 gtagctcgct cctgtggacg tcactactcc atgacaaaca ccatcttcga cgatcagttc
    12481 ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt tcgatcgtat gtttcatttc
    12541 tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct cggacagctg ggccttttcc
    12601 tgtgttcaac atacgcattt ggatataggt tttatcgata ttcttaccca tttctccgcc
    12661 taacgcatcg atttctcgaa cgacaacgcc tttagctggt ccgcctacag aaggattaca
    12721 tggcatgaat gctaccatgt ccaaatttat tgtcagtaat aatgtttttg atcccatacg
    12781 tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga tcacgtcata
    12841 cgcatctgct tcataatgtt ccaatcgttt gttcctcctt ttattttcct aaacaaaatt
    12901 gactgaataa ttgagtgatt agctcatctt gtacactgtc gccgacaact tctcctagat
    12961 agtcccagca acgcgtcata tctatttgaa cgagatccac aggcattcct gaatcaatac
    13021 ctgcaatgac ttcttcaagt gagagggaag ctttttctaa caacgcgata tggcgggtat
    13081 tagaaacata agtagcgtct ctttctcctg tttctccgct gaagaataaa tcagaaatag
    13141 ctgattccaa acgatctagt ccgtcgtttt tagctacgga aattgaaaaa actggttcat
    13201 tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct gttttattta
    13261 gaagaatgat ccgtttcaat ccacttgtcg cttccaataa ttgttcatct tctgctgtca
    13321 gtggttcgct ttggtttaga acaagtaaga tcaagtctgc ttctgctaat gcttttcggc
    13381 ttctttcgac cccgatccgt tccaccacat cttctgtttc ccgaattcca gctgtatcga
    13441 ttagcttgag cggtaccccc cgaacattga catactcttc gattacatct cgagtcgttc
    13501 ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa atggtttaac aaacttgatt
    13561 tgccgacatt tggccggccg atgattgccg tacttaaccc ttcccgcaga accttccctt
    13621 gcttggatgt agcaagtagc gcttggattc tttgttggat catctgcgct ttttcaagca
    13681 ataacttcgt agtcaattct tcgacatcat catattctgg ataatcaata tttacttcta
    13741 cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca gacagattgc
    13801 cgtctaactg attaagagct agtcccattg ctttgtctgt ttttgcccga atcaaatcca
    13861 tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt tttgtgaatt
    13921 cgcctggctc agctaatctt gcaccttctc ttaacaatag ctgcaaaatt tgattgacta
    13981 caacgatccc accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat gtctttggtg
    14041 ctctcatcac ggagaccatg acttcatcga caagctgatt gctcttggga tcaacgatat
    14101 gtccataatg gatcgtatga cttggtactt ctgataatcg tttgtttcca gattgataaa
    14161 ctttatctgc tagttgcaca gcttgatcgc cacttaaacg aacgatactg attgcacctt
    14221 ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt tcagccattt
    14281 tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc actccaccct ttatgcacaa
    14341 taaacggtga acttcgcact ttgactgctt gttcttgatt aaattctggt catagattat
    14401 cacacttgct ataaggaatc aagtgtttaa aacaattaga tgaagttgag cctgttctgc
    14461 ttccattacc ttatatttaa ttgtccttgg atgtctcttt gcgttctttg gtttattcga
    14521 agagaagata ctgtataaac aaggctttat ctttcttaaa aaaaacggta cactaaatgg
    14581 tagcgctatc taaactatac acttttgaaa ggagaaaata tgtgagcccg ttcagatctg
    14641 ctgcattgga agaagaaagt gaccctcgga tcaacgatgt cgttttatct acagggaata
    14701 tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt ggaatgctta gaagctgatt
    14761 tgtttgaccc atcttttccc aaagatgacc ttttcttaac aacgaagagg aaattaaaga
    14821 cgaaggtact tgaatatggt gcgaatgcag taatcaactg tcattttgag tatcagcaaa
    14881 aaaaacgcga cggaaagtat tttctggaac tttttgctta tggaacagtg atccaattta
    14941 tccaaacaac gattggttaa atgaaacaga caaaaaaagc ggatcaaaac tgaagacagt
    15001 ttcgatccgc ttttttctat caaagttagt gactacttca tcgtatactc tgataagaat
    15061 gatttctagt aaaatatact tggtttgaaa aactttgtca acgtgattta ttcaaaaagt
    15121 aatggaatca ataatatttt ttagcaggtt ctactactaa ataacgataa ggttcctcac
    15181 cttccgaatg tgtttttaca tgttcatctt tgcttaatgc agcatggatt tgtttacgtt
    15241 caaatgcagg cattggttct aagaaaacag gctgccctgt ccgttttact ttttcagccg
    15301 tgcgatcagc taaacgctga atgatctctt ggcgcttttc acggtaattg cctacattga
    15361 ctactacaga cagcttattt cttgctaaac gatggatgaa gacttgtgcc agatattgca
    15421 atgcattcag tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt gtttctaaat
    15481 ggaaaacaac catattattt gattctcttt ccattttgac aagagctgga gcatcaagct
    15541 gttttgaaat ttcagtaaga taaactgcca attccgttaa agctgcttca tcacctagct
    15601 cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg atctcagctg
    15661 ttggttcttc ttccaaaatt tcttctacag tctgttctac tgtttcagaa actttttcgc
    15721 tgatacttgg ctggatcgaa agccgtgcat tctttcgacc cattcctagg aaaccttttt
    15781 ttccttcttc cagtatttcg atttcgactt ggtctttggc caaacccaaa tcagccagac
    15841 ctagttggac agcttcgtct attgttgcag cttcataaat cggcattagc attacctccc
    15901 ctattatttc cgttttttct taggattcat tgctttctta agagcacgtt ctctttcgcg
    15961 gattctccgt gcttcttctt cacgctcttt tcgaattttg aaagggttgt tgatcagcag
    16021 cgtttgtaca acttggaagg cattggaaac tacccagtat aatgaaagac cgctggctaa
    16081 attgacaccc atcactaaaa tcattacagg catcacgaaa ttcatgatct tcattgatgc
    16141 attcgattca atttgactca tgctagataa atacgtactt gcaaaagtaa agattgctgc
    16201 taaaatcggc aaaataaatg ttggatccgc gttccctaag ttcaaccata agaaatgccc
    16261 ttgtttcaac tctggtacac gtgaaataga ttgccataac gccatcaaga ttggcatttg
    16321 tactaataaa ggaagacaac cagcatatgg attgacgcca ttttctgcat ataaacgctg
    16381 ctgctcttct cttaacttac tttgtgtttc cggatctctt gaactatatt gttgctgcag
    16441 tgcttttaat ttcggttgta attcttgcgt tttacgcatg ctctttgttt ggaaatgcat
    16501 caacggcaat aggatcacgc ggatgatcag tgtaaataaa atgatcccta tccctgcatt
    16561 accaaatgac aacgccttga tcgcctcagc aaagtaataa acaatataac gatcccaaat
    16621 tcctgtactc tgtgcgctga cttccccagt tccgcaaccg gataagacga tgaccaaact
    16681 aacgataccg gccatcaaaa gtaaacgttt gtatttcttc acttactctt ttcctctctg
    16741 tcgattattt ttgcaagttt taagacgtgg actaaattgg agcgaacctc atcataagtc
    16801 aaccccttca cacttggtct agcaatcaca ataaaatcca actccggatc aatttcttct
    16861 tttatagaat aaactgctgc tcggattttt cgtttgactt cattgcggtt taccgcgttg
    16921 ccgacctttt ttcctacaga taaaccaact cgaaaatgag gctgtcccgg tttttccaaa
    16981 cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg tatggaacac ggcttgaaac
    17041 tctcgttctt tttttactcg atacgctttt ctcattagtt ttctttcctt ttctatctct
    17101 cacttacgct cataatcata ccataattac gattgtcctt tcatcatttc cctaaaaaag
    17161 agtataaaaa aaaccactga aaccccagtg gcctaagctg aaagaacttt tcttcctttg
    17221 cgacgacggc tagctaacac acgacgtcca tttttagtac tcatacgttt acggaatccg
    17281 tgaacttttt gacgtttacg tttatttggt tggtatgttc ttttcatata ttccacctcc
    17341 aaaatgttct tatctatttt ataaacacag acatactttg atagtataac gacaaagtag
    17401 tgattttgtc aatataaaat agaagatcga ttgttaaaac ttatccacaa gattattttt
    17461 ttctatggga atcctatgtg gaaaaagtta tgcacaggga ttatccacag ataataaaag
    17521 gctggtttta cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag gcgatttcct
    17581 gtgtattccg tctcctaact attggtatat ctgtattttt tatccacaga cgcaagagga
    17641 aatctattgg tttttacgag ttttccacct gtgcattagt ggtgtggaaa actttttttg
    17701 acaactgtgt ttttctcttt tttctttttg tcgattgtgt ggataattgt aattaaaaaa
    17761 tttttttctt cggtttctgt ggaaaactat tgaaaaaaat gctaaattag tactagttat
    17821 tcacttttta atgaaggagg aggccttata tggtatccct cgatgcttta tggaatgaat
    17881 taaaagcaac ataccaaaag gatttatcgc ctgctagcta taatacatgg atcgaaacag
    17941 cgaaccctcg aacccttgat caaaaccagc tcgttgttga ggttcctagc aagattcata
    18001 aagaatattg ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat atgttatcag
    18061 gaaatgagat tatccctcgc tttattactg gcgaagaagc agagcaagaa gaagttatag
    18121 aagaaaaaaa tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg ctgaacccta
    18181 aatatacctt tgatacgttt gtcattggta aaggaaacca gatggctcat gcagcagcgc
    18241 tcgttgtagc agaagatcca ggttctattt ataacccgct cttcttttat ggaggcgtgg
    18301 gacttggtaa gactcacttg atgcatgcaa ttggtcatca aatgctgcaa agtcagccta
    18361 atgccaaagt aaaatatgta agcagtgaaa cttttgctaa tgatttcatc aattcgatcc
    18421 aaaacaaaac agcagaagag ttccgtcaag aatatcgaaa tgttgattta ctgctagtgg
    18481 atgacattca attttttgca gaaaaagaag ctacgcaaga agagtttttc catactttca
    18541 atgccttgta caatgaaggg aaacaaatcg tactaaccag tgatcggttg ccaaacgaga
    18601 ttccgaaact gcaagaacgc cttgtttctc gctttgcatg ggggctgtct gttgatatta
    18661 caccgccgga tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg gctgaacgtc
    18721 tagagatccc agatgacaca ctaagctaca tcgcaggaca aattgattct aacattcgtg
    18781 aattagaagg tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt gaagatattt
    18841 cgactagtct agctgcagac gcactgaaaa cgttaaaatc aggaaaagga catcctcaat
    18901 tgtcaatcct gcagatccaa gaagaagtgg caaagtatta tcacatccaa ttgaaagatc
    18961 tcaaaggaaa gaaacgcgtc aaatctattg ttgttcctag acaaatcgct atgtatttgg
    19021 cacgtgagct cacagataat tctttaccga aaatcggagc tgaattcggt ggcaaggatc
    19081 atacaacggt tatccatgct catgaaaaaa tccagcagct gatggattct agtgtcagta
    19141 tgcaaaatga agtatcagaa atcaaaaact ttttactaaa ttaatttgtc gattctatca
    19201 actgaaaata gcatgtggat aaaagaaaaa aacgttcaaa agttatacac actttttcca
    19261 cagctgtttt ttcgtttctc tcttacgttt tttgagtttt ccacagaatc aacatgccct
    19321 attactttta ttattctttt taactaataa taaataaaca aacattcaaa ggagatacat
    19381 catgaaagtt actttaaacc gagctagctt tatgcaggaa ttgcaaactg ttcaacgagc
    19441 tatttcaagc aaaaccacga tccctatttt gacaggtgta aaaatcacac tgacacaaga
    19501 aggtttgact ttgacgggga gcaacgctga tatatcaatt gaaacttttt tgtctgttga
    19561 aaacgaaaaa gcaaatatgc aaatcgaatc tactggttcc attgttttac aagcacgttt
    19621 ctttagcgaa atcattcgga gacttcctga agaaacattt actttagaag ttttagaaaa
    19681 taaacaagta gcgatcactt ctggaaaagc gaattttatc gtaaatggat tagatgcaga
    19741 taactatcct catcttcctg ttgtcgaaag ccataaccag atgaaattac ctgtacacgt
    19801 attgactaaa ctaatcaacg aaacagtttt tgctgtctca caacatgaga gtcgtccaat
    19861 cttgacaggt gtgcatttca ttttatctga taattcttta ttagctgtag ctactgattc
    19921 tcaccgtcta agtcaacgcg tgattccagt agaacaagcg gctgatcatt ttgatattgt
    19981 tattcctgga aaaagtttga tcgaattatc tcgctcatta acaaatgaag aagaaatcgt
    20041 cgaaatcagc attatggaaa accaagtgtt gttcaaaaca gaaacgatgt atttctattc
    20101 ccgtttgcta gaaggaaact atccagatac caatcgtttg attccttcaa gttttaatac
    20161 agaggttgaa ttttctgttc ctagcttttt agcggcgatc gaacgggctt ctttactttc
    20221 tcatgaaggc cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg ttgttttata
    20281 cggaaattcg ccagaaatcg ggaaagtaga agaaagcttg agttatacag caagcagtgg
    20341 cgatccatta gatatttctt tcaatccgga ttatatgaaa gcagcgttgc gtgcatttgg
    20401 agatatgagc atcaaagtga aatttatctc tgctattcgt ccatttacat tggaaccaac
    20461 ggaagatggc gttcaattta ttcagctgat tactcctgta cgtacaaatt aacaatgatt
    20521 ttcaatgttc ttgaaaattt aattgaaaag aaatagaaaa aataaaattt taaacagaag
    20581 gccctaaaat ctcttttaag gctttctgtt tatttttgtt taaatcacct tttttagatt
    20641 aaatcgtctt atatggaaaa atatatggat aaatttgttt tattgcgaaa aaaaaggtat
    20701 aatagagtat acttttaata atgaaaatga gggatgggca ttgaaacaac aagtcattct
    20761 aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg
    20821 cggacaagca aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa
    20881 tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa gttccagaag taggaacttt
    20941 ttttatggta aaaaaaggaa acgacgataa tgaggctgaa taagctgtat ttaaaaaatt
    21001 atcgaaatta cgaagagctg aacatcgaat tttccaagaa cctcgtcatc tttttaggag
    21061 aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta cgtattagct atgacgcgaa
    21121 gtcatcggac aagtagtgag caggaattga ttggctggac tgacgatcag gcgatgattc
    21181 aaggagagat cacaaaaggt tcatccactc ttccattaga aatcttatta tctaaaaaag
    21241 gccgaaaaac caagatcaac catatcgaac agaaaaagtt aagtacttac gtgggacaaa
    21301 tgaatgtcat tttatttgca ccagaagatc tttctttagt aaaaggcagc ccgcagatca
    21361 ggcggaagtt tttagatatg gaattaggcc agatcaatcc aatctatctt tatgatcttg
    21421 tccaatacca agcaattttg aaacaaagaa accagtactt gaagcaattg aatgaaaaaa
    21481 aacaaactga tttgctttat ttggatattt tgtccgaaca actagcagcg tttggcagca
    21541 aagtattgaa agcaagagca cagttcgtca agcgattgga gttttgggct aattctttgc
    21601 atcagcaaat cacccatcaa aaagagcagc tggaaatcga gtatctcacc gcagtcgatt
    21661 ctttagaaac acatacgcaa gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa
    21721 ataaaaagaa agaccttttt cggggaacaa cgacagttgg tcctcatcgt gacgatcttt
    21781 ccttttttat taatcagaaa aatgtgcaga cttatggatc gcaaggacag caaagaacaa
    21841 ctgcactaag tgtcaaatta gcagaaatcg atttgattaa agaagagaca ggggaatatc
    21901 caatcctttt gttagatgat gtgatgagcg agctggatga taatcgtcaa ctccatctgc
    21961 tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt gaccatgtaa
    22021 aaaataaaat gactgttgaa cctgagattt tttatgttca acaagggaaa ctaaaaggag
    22081 aagaaactga atgacagaag aaagaagttt agtagaacgc gctaaagagt atgatgccag
    22141 tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg
    22201 ttcgactagt tctgagggtc tgcatcacct tgtttgggaa attgtcgata attcgatcga
    22261 tgaagtatta gcaggttttg caacgaaaat ccatgtgatt attgaaaaag acaacagtat
    22321 caccgttatt gacgacggtc gggggatacc tgtagatatc caggcaaaaa ctggacgtcc
    22381 tgcagttgaa acggtcttta ctgttttaca tgctggagga aaattcggcg gtggtgggta
    22441 caaagtttct ggtggacttc acggagtagg atcttctgtt gtaaacgcgc tttctacaca
    22501 gcttgatgtc aaagtataca aagaaggaaa tgtgtactat caagaatatc gtcgtggagc
    22561 tgtagtggac gacttaaaga ttatcgaaca aacggatcgc catggaacaa cggttcactt
    22621 tacgccagac cctgaaattt ttacagagac aactgagttt gatttttcaa aattggctac
    22681 ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt atatcgattg aagacaaacg
    22741 agaagaagaa ccagtcatca atgaatacca ttatgacggc gggatcaaaa gttatgtcga
    22801 atacttgaat gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg aaggtgaaca
    22861 gcaagatatc gcagttgaag tctctatgca atatacagac ggctaccatt ccaatatcat
    22921 gagttttgcc aacaatatcc atacgtatga aggtggaaca catgaatcag gattcaagac
    22981 tgctttaact cgtgtgataa atgattatgc ccgaaaacaa aagctgatga aagaaaatga
    23041 cgacaacttg accggtgaag acgtgcgaga aggattgaca gctgttattt ctatcaagca
    23101 tcctgaccct caattcgaag gacaaacaaa aacaaaattg ggcaattccg aagtacggac
    23161 agtcactgat cggttattta gcgaacattt catgaaattc ttgctggaaa acccaagtgt
    23221 gggccggcaa attgttgaaa aaggattact agcttccaga gctcgtctag cagcgaaacg
    23281 tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt agtaatctgc caggaaaatt
    23341 agcggactgc tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc
    23401 ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt caagccatct tgcctattcg
    23461 tgggaaaatc ttgaatgttg aaaaagcaag catggacaag atcttggcaa atgaagagat
    23521 ccggtcttta tttactgcta tgggtacagg gtttggagaa gattttgatg tatcgaaagc
    23581 tcgctaccac aaattagtta tcatgactga tgccgatgtc gacggcgcgc atatccgtac
    23641 attgctgctg actctttttt atcgttacat gcgtcctatt gttgaagctg gatacgtcta
    23701 tattgctcag ccacctttat acggggtaaa acaagggaag aatattacgt atgtacaacc
    23761 aggaaaaaat gcagaagaag agttgaaaca agtcgttgct tcacttcctg cctcaccaaa
    23821 accaagcgtt cagcgttata aaggacttgg agaaatggat gatcatcaac tatgggaaac
    23881 aactatggac ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc
    23941 agatcagata tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg cattcatcga
    24001 ggaaaatgca cactatgtga agaacttaga tatttaatca tgaaggagga aaaagaatga
    24061 gtgaagaaat cagagaaaac atccatgatg tcaatctaac cagcgaaatg aaagactcct
    24121 tcatcgatta tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc agagatggtc
    24181 tgaaacccgt tcatcgccgt attttatacg gcatgaacga attaggtgta acaccggata
    24241 aagcccataa aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac catcctcatg
    24301 gagacattgc gatctatgag tcaatggtac ggatggcaca gccattcagc taccgttata
    24361 tgctagtgga tggtcatgga aactttggtt ctgttgacgg tgacggtgcc gccgctatgc
    24421 gttataccga agcacggatg agcaaaatcg ctacagaaat gctgcgtgat atcaataaga
    24481 acactgttga tttccaaagc aactatgatg atacagaaaa agaaccagtt gtactacctg
    24541 ctcgcttccc gaatctttta gtgaacggaa caacggggat cgccgtaggt atggccacga
    24601 atatcccacc tcataacttg tctgaggtag tagcggctat cgatttgctg atggataatc
    24661 ctgatgtgac gaccaatgaa ttgatggaag tattacctgg accagatttt ccaacaggtg
    24721 gtttggttat ggggaaatca ggaatccgaa gagcgtatga aacaggtaag ggttctatta
    24781 ccgtacgtgc aaaagttgag attactgaga tgccaaatgg taaagaacgg atcatcgtga
    24841 cagaattgcc ttatatggtc aataaagcaa aattgatcga acggatttct gaactgcaca
    24901 gagacaagcg tatcgaaggg atcacagatt tgcgggacga atcttcccgt gaaggtatgc
    24961 ggatcgtcat tgacatccgt cgtgacgcca gcgcatccgt tatcttgaat aacttatata
    25021 aattgacatc cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc
    25081 cgaaagtttt gagcttgaaa caaatccttg aaaactatat cgaacatcaa cgcgaagtca
    25141 tcacacgacg gacggtcttt gaaaaagaaa aagcagaagc aagagcacac atcttagaag
    25201 gtttgcgtat tgctttggat catatcgatg aaatcattgc gattatccgc gggtctaaat
    25261 cagatgacga ggcaaaagca acaatgatcg aacggttcga tttatccgat cgccagtctc
    25321 aagctatttt agatatgcgt ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa
    25381 atgaatacca agaactctta aaactgattg aagatttaac tgacattctg gcaagaccag
    25441 aacgtgttac agaaatcatc aagaatgaat tagcagactt gaatcaacga tttggtgata
    25501 aacgacgcac agaattacta gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg
    25561 aagaagaaga aatcgttatt acattgacaa acaatggtta catcaaacgt ttggcaaata
    25621 atgaattcag agctcaacga aggggtggac gtggtgtcca aggaatgggt atccacaatg
    25681 acgacttcgt caaaaatctg gtttcttgtt ctacccatga tacattgtta ttctttacga
    25741 ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc agaatacggg cgtacagcca
    25801 aaggaatccc aatcatcaat ttactaggta tcgattcgag tgaaagcatc caggcaatca
    25861 ttgcggtgac gggagaagca gaagaaggcc actatctctt ctttacgacg cgtcaaggta
    25921 cagtaaaacg tacttctgtc actgcttttg ctaacatccg aagtaatggt ctgatcgcaa
    25981 ttggtttgaa agaagacgat gagctcgtca atgtgctact gacagatggt caatctaata
    26041 tcattattgg aacacataac ggctactctg tcactttcaa agaagaagca gtccgcgata
    26101 tgggacgtac agcttccggg gtcagaggga tacgcctgcg tgaggaagat tatgtcgttg
    26161 gggcagcctt aatgaaggaa aatcaagaag tattagtgat cacagaaaaa ggttacggaa
    26221 aacggacaaa agtatcggaa tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg
    26281 cgaacatcac tgaaaaaaat ggtccactag ctggattgac cactgtttct ggagatgaag
    26341 acatcatgct gattacagat aaaggtgtca ttatccgttt caacgtatcg actgtttctc
    26401 aaactggacg ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg
    26461 tcacgatggc agcagtcgaa ccagaagctg cagaacaaac agttgaagaa caaggatcag
    26521 aagaataacc atcaagacta agtgtgtaag ctagcaagaa taaccgatta aaaaatctgg
    26581 ttattcctgc tggctttttt gattagttga atttctctta aaatattcga aactgttcat
    26641 tctgtattgc ttttgaccaa aatagagtgt ataattagag cttgtgagta gtctaattaa
    26701 ttagataact ctccttgctc ttaggaacct aagagccgaa agtccacagg gaggtgaata
    26761 tcaatggaaa acacgaaata tgaaatcatg tacattattc gtccaaacat tgatgaagaa
    26821 gcaaaaactg ctttaatcga acgtttcgat gcaatcttga aagataacgg agcagaagtt
    26881 atcgaatcaa aagattggga aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa
    26941 ggtatctatc acatcgttaa agtatcttct ccatcatcag caaatgctgt caatgaattt
    27001 gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa agaagaagct
    27061 taattgttgt ttcatgtgaa acaatagaaa acgatgagag gagatgacta ttttgattaa
    27121 taatgttgta ttagttggac gtctgacgaa agatccagat ttgcgttaca cagcaagtgg
    27181 tacggcagtc gcaacattta ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa
    27241 tcgcgaggct gattttatca actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa
    27301 ctatgctcgt aaaggaacac ttttaggtgt tactggacgt atccaaactc gttcttatga
    27361 taatcagcaa ggacaacgtg tatacgtgac agaagtagta gctgacaact tccaattgct
    27421 ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc ggattccaaa gttctggtca
    27481 atcagctggc ggatttggcg gaaacaataa ctctaatcaa acatctcaat catccaacgg
    27541 tatgcctgat ttcgaccgtg atacttctga cccattcggc tcaagttcaa cgatcgacat
    27601 ttcagacgat gatttaccat tctaaacgaa tttgatagga gggaaataga atggcacaac
    27661 aaagaagagg cggacgtaaa cgtcgtaaag tagactacat tgctgctaac catattgaat
    27721 atatcgatta taaagatatc gaattactaa aaagatttat ttcagaacgc ggtaaaatct
    27781 tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg taaaattact gttgcaatca
    27841 aacgcgctcg tatcatgggc ttgctacctt tcgttagcga agactaataa atcctaggaa
    27901 ttgaatgact gatccttttg atcagctatt caattccttt ttttctgtaa aaataacctc
    27961 tattcgttta gtcgctcgaa tgacgaaagt tatggtaaaa tcaaattagt catatatggg
    28021 aggaaaatta tggaaaataa aaacaatcat ttatccccga aacctctctt ccttatcctg
    28081 tttatattaa tcgaagtatt ggtagtctta attttaccat ttcgttttgt tagcgcttcg
    28141 gtcattctat tgatgaattt atggggtgtt cgctattggt tgaaaatgaa acgttggctt
    28201 attttaaata aacgtgaaaa aatccagcag gcttcgactg tagctgaaac caatctagct
    28261 tatgtctcag aagtcatgcc tgtaggagtg attgcttatc agccatcaag ccaaaaaatc
    28321 gaatggctca atccattagc attgaaaatc aaagatcaaa gtggattaac agaggaagaa
    28381 ttgctagata ctttttttac tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat
    28441 aaaacatttc agtttgatat ggatacagta aagggtgttg tttattttaa tgatattacc
    28501 caaaaccagt tgttaaaact ccatcagacg aacagtcagc cggtgattgg tattttatct
    28561 attgataact atgcagatgc cattgataaa ttagatgaca aagagatttc ttatttgaac
    28621 tctttcgtca ctacactagt ttctgactgg atgaatgaat accatgtctt tttcaaaaga
    28681 atcaacgcag aacggttttt cttcgtagca cagatgatcg atctgaaaaa gatgatggat
    28741 aaaaaatttg atttgctgga tcgctttcga gcagaagctg aaaaacagag ctcgcctatc
    28801 acccttagta tggggattgc ttatggctca agaaccctcg atgaaatcgg tggagaagct
    28861 cagaataatt tagatgttgc tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta
    28921 aaagaaggag caaaacctgt ttattatggg gggaaatctg ctaagaatgt caaacgcacg
    28981 cgtgttcgtt ctcgagcgat gagtaccgct ttgcgtcgga tttttgatga aacaggcgat
    29041 gtgtttatca tggggcacaa gtttccggat atggatgctt taggctctgc ttttggtgtc
    29101 agttgtctgg cacgcttcaa ccagaaaaaa gcttacatcg ttatcaatga gaacgagatc
    29161 attccagatg tagaacggtg tcttgaagaa atccataagc atcctgaatt agaaatgcaa
    29221 ctaatctctc cagaacgtgc catagagctg aaaaaagaag aggacctcct cgtgatggtg
    29281 gattaccata aaccttctct atcgatctct cagggcctat atgagcaatt tgataagatt
    29341 gtgatcattg atcaccatcg tcgtggggaa gagtttccaa gtaaacctct gctgacttat
    29401 attgaatctt cagcttcttc agcttcagag ctagtttctg agctgatcca ataccaaagc
    29461 agcaaaacaa atcgcttaga tcgttttgag gccacattat tgcttgcagg gatggttgta
    29521 gatacaaata gtttcaatgt ccgtacaact gcacgaactt ttgacgttgc cagctattta
    29581 aggtcttgtg gagcagacgc atccttagta caatatttat tgagctcgga tttagcgaca
    29641 tacttggaga tcagcaattt gattgctgga agcgactatg tgacttctga tattgttgtg
    29701 gcggcaggga cagacgaaaa agagtataat agtgttatag cagcaaaaac agcagataca
    29761 ttgctttcga tgatcaatat caatgcagct ttcgtcatta caaagcggac agacggcttg
    29821 attgggatca gtgccagaag taccggcacg atcaacgtcc aaatcatcat ggaatcctta
    29881 ggtggcggag gacacttcac aaacgcagct actcaactgg aaaatacgac agttgaggaa
    29941 acaaaacaga agctatttgc tgcaatcaaa gaaaatatga atcaaatata tgataaggag
    30001 taatccaaat gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca
    30061 tcaaagaagt tccagcaggt tatgcacaaa attatctaat caaaaataac ttggcaaaag
    30121 aagcaacgaa aagcagtatc agtcagttag caggacaaaa gaaagcacaa gaaaaacacg
    30181 aagctgaaat cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa gaagatactg
    30241 tagtcgaatt gaaggcgaaa gcaggggaag acagccgttt gtttggttcg atcccttcaa
    30301 aacaaattgc tgaagggtta aacaaacaat acaaaatcaa attagataaa cgaaaaatcg
    30361 aattagaaaa tccgatccgt tcactaggct acacaaacgt acctgttaag cttcatcatg
    30421 aggtgacggc tacgatcaaa gttcatgtcg tacaagaata acaaatcttg atagcgatgt
    30481 tcagctggta agtataacca gaaggaaaaa agacaaaccg aaatttatct gagaaaacag
    30541 ctgcagcaat gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt ttcttttttg
    30601 ggaacagcaa aatagcataa agaaaacgag tataataaaa gagaactatt tttttattac
    30661 actttcggta cgaatgatga cttttctctc attttcttgt aaaatgaagc aattgaagac
    30721 aaggagcgat acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa aatattgaag
    30781 ctgaacaagc ggttttaggc tctgtttttc ttgacgcaga aacgatcatt gatgccatgg
    30841 agtacataga gccaaaagac ttttatcgtc gtggccatca aattattttt gagacaatga
    30901 tcgagttaaa tgatcgtaat gaagcgattg acgtggttac tgtcaaagac cggctggaac
    30961 aagccaattt gttagaagat gccggtggtt taagttatct ctcagacttg gcattagcag
    31021 ttccgacagc agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc
    31081 gtacattgat ccagacagca acaggaatcg ttacgaaagg attcgagcaa ggagaagaag
    31141 ttcaaacaat tttagatgat gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc
    31201 gaagcggctt tttatctatt gctgaagtac tgaactccac gattgaaaat atcgatcgac
    31261 tagctcaaaa caatgaagaa atcacaggtc taccaactgg ctatcaaggt ctggataaaa
    31321 tgacagcagg tttccagccc gaagaactaa tcattcttgc tgcacgtcca gcggtaggta
    31381 aaacagcgtt tgcattgaat attgctcaaa atgtaggaac aaaaacagat cgttctgttg
    31441 cgatatttag tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag
    31501 gttcgattga ggccagccat ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc
    31561 tgatcgttgc gatggggagt ctttctcgag caaatatttt tatcgatgat acaccaggga
    31621 tcaaaatctc tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag
    31681 gcttgatatt gatcgattat ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac
    31741 aagaagtatc agatatctct cgccaactga aaaaactggc gaaagagctg aaagtacctg
    31801 tcattgctct ttcacagtta tctcgtggag tagaacagcg acaagacaaa cgaccagtgc
    31861 taagtgatat tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta gccttcttgt
    31921 atcgagatga ttattatgaa agagaaggcg gagatgacga tgatggaaac gagccaaaaa
    31981 atgacaacgt catcgaagtc atcatcgaga agaaccgaag cggtgcacga ggaacagttg
    32041 aactactgtt tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga gaagaatttt
    32101 aaagaagagg ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt
    32161 aaccgcagga acgactagag tacttaattt aaacagatct ccaaagggat tgtgacaaaa
    32221 gtcttgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgacc cttggtatta
    32281 gctcagcaaa cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt
    32341 caaaacgctg atcttacgac ctcttttcct taaacggtgt tctcctgcca actcctcggt
    32401 cacaagtcac aatatcatca aacatgagaa gcagtttttt gaaattgctc cttgtgactc
    32461 agagttaaac ggcaggctca caacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    32521 ggtaatacgc ccaatcaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt
    32581 acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaaa agtagcttct
    32641 tcgaaaaata agtcgaaatt tcacaaaaat ttgagaagca attttcgtac attccttctt
    32701 atttcttgaa gctgaccact tctgttacaa cctctttttt aataaccatt ctaaaaagat
    32761 aaagttcgtt ttttaagcgt ttttataaaa taaaaataaa aatcattcga ttttatttga
    32821 aatatgtatc tattattgat acaatggttc aggattaaat aatatagaac gaggtgttca
    32881 catgtcatct gtagtagtag taggtacgca atggggcgat gaagggaaag gaaaaatcac
    32941 agactttctc agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg acaatgcagg
    33001 gcacacaatt aagtttaacg gtgtgactta taaactgcat ttgattcctt ctgggatttt
    33061 ttataaagac aagatcagtg tgatcggaaa cggcgttgta gtcaatccta aatcattggt
    33121 taaagaatta gcttatttac atgaaaaagg tgtagaaaca agtaatttgc gtatttctga
    33181 ccgcgcgcac gtaatcttgc cttatcatat caaattggac caattacaag aagatgctaa
    33241 gggcgaaaat aagattggta caacgattaa aggaatcgga cctgcctata tggataaagc
    33301 agctcgtgta ggtatccgta ttgcagactt attagataaa gaaatttttg aagaacgtct
    33361 tcgtttgaat ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt cagaaccact
    33421 tgattttgac gatatttttg aagaatatta tgaatacgga caacaaatca aacaatacgt
    33481 cacagatact tccgttatct tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga
    33541 aggcgcacaa ggtgtaatgc tggatatcga ccagggaact tatccttttg ttacttcttc
    33601 taacccagta gccggtgggg taacaatcgg aagcggtgta gggccttcaa aaatcaataa
    33661 agttgtcggc gtctgcaaag cctatacgtc acgtgtcggt gatggaccat ttccaactga
    33721 attatttgat gaaacaggag aacaaatccg cgaagtagga cgagaatacg gtacgacaac
    33781 tggtcgtccg cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt ctaaacgagt
    33841 ttcaggtatt accaacctat ctttaaactc gatcgatgta ttaagtggat tggaaacagt
    33901 aaaaatttgt acggcttatg aattagatgg cgaactaatc taccactatc cagcaagctt
    33961 gaaagagttg aaccgctgca aaccaatcta tgaagaattg ccaggctggt cagaagacat
    34021 tacaggatgc aaaacacttg cagaactgcc agaaaacgca cgtaactatg tacgtcgtat
    34081 ctcagagctt gttggtgtac gtatctcaac attttctgtc ggacctgata gaacacaaac
    34141 aaacatctta gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac tgaatcagga
    34201 cgttcttacc agagcgagtc gaagtgttta tctatacgat aataataact tgaagaggta
    34261 gtgaaagaga cgtttagcct tattgccgat aggctgtctc ttgaacactg tttattcaga
    34321 ataagggact gtgacaaaaa ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAYYHB010000024        33860 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000024 DAYYHB010000000
VERSION     DAYYHB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.024"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHA6B_001087"
     CDS             complement(1..120)
                     /locus_tag="ACHA6B_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8948676.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHA6B_001088"
     CDS             299..1960
                     /locus_tag="ACHA6B_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8948677.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ACHA6B_001089"
     CDS             complement(2030..2377)
                     /locus_tag="ACHA6B_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8948678.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /gene="opp3b"
                     /locus_tag="ACHA6B_001090"
     CDS             2461..3402
                     /gene="opp3b"
                     /locus_tag="ACHA6B_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8948679.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ACHA6B_001091"
     CDS             3403..4446
                     /locus_tag="ACHA6B_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948680.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ACHA6B_001092"
     CDS             4452..5522
                     /locus_tag="ACHA6B_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948681.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ACHA6B_001093"
     CDS             5512..6465
                     /locus_tag="ACHA6B_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948682.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ACHA6B_001094"
     CDS             6477..7487
                     /locus_tag="ACHA6B_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8948683.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ACHA6B_001095"
     CDS             complement(7772..8593)
                     /locus_tag="ACHA6B_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8948684.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ACHA6B_001096"
     CDS             complement(8690..8968)
                     /locus_tag="ACHA6B_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948685.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ACHA6B_001097"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ACHA6B_001097"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8948686.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDLVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ACHA6B_001098"
     CDS             complement(11724..12311)
                     /locus_tag="ACHA6B_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948687.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ACHA6B_001099"
     CDS             12486..13808
                     /locus_tag="ACHA6B_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8948688.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ACHA6B_001100"
     CDS             13963..14739
                     /locus_tag="ACHA6B_001100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8948689.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ACHA6B_001101"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ACHA6B_001101"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8948690.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ACHA6B_001102"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ACHA6B_001102"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8948691.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ACHA6B_001103"
     CDS             16629..17258
                     /locus_tag="ACHA6B_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948692.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /locus_tag="ACHA6B_001104"
     CDS             complement(17382..18491)
                     /locus_tag="ACHA6B_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948693.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ACHA6B_001105"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ACHA6B_001105"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8948694.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19309..19407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19445..19981
                     /locus_tag="ACHA6B_001106"
     CDS             19445..19981
                     /locus_tag="ACHA6B_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8948695.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ACHA6B_001107"
     CDS             complement(20021..21784)
                     /locus_tag="ACHA6B_001107"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948696.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ACHA6B_001108"
     CDS             complement(21787..23502)
                     /locus_tag="ACHA6B_001108"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948697.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="ACHA6B_001109"
     CDS             complement(24281..24505)
                     /locus_tag="ACHA6B_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948698.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="ACHA6B_001110"
     CDS             24696..27473
                     /locus_tag="ACHA6B_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8948699.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="ACHA6B_001111"
     CDS             27554..27973
                     /locus_tag="ACHA6B_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8948700.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="ACHA6B_001112"
     CDS             27985..28455
                     /locus_tag="ACHA6B_001112"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8948701.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="ACHA6B_001113"
     CDS             28472..29251
                     /locus_tag="ACHA6B_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8948702.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="ACHA6B_001114"
     CDS             29238..30059
                     /locus_tag="ACHA6B_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8948703.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="ACHA6B_001115"
     CDS             30076..31125
                     /locus_tag="ACHA6B_001115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8948704.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="ACHA6B_001116"
     CDS             31138..32142
                     /locus_tag="ACHA6B_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8948705.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="ACHA6B_001117"
     CDS             32775..33794
                     /locus_tag="ACHA6B_001117"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8948706.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11056 a         5995 c         6918 g         9891 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga tcttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatatgaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttagtctaat ggtgataagt
    33841 caagatgaaa tttaagattt
//
LOCUS       DAYYHB010000025        29472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000025 DAYYHB010000000
VERSION     DAYYHB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.025"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2067)
                     /locus_tag="ACHA6B_001118"
     CDS             complement(<1..2067)
                     /locus_tag="ACHA6B_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8948707.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTS"
     gene            complement(2080..2355)
                     /locus_tag="ACHA6B_001119"
     CDS             complement(2080..2355)
                     /locus_tag="ACHA6B_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948708.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2717..3172)
                     /locus_tag="ACHA6B_001120"
     CDS             complement(2717..3172)
                     /locus_tag="ACHA6B_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948709.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3326..4534)
                     /locus_tag="ACHA6B_001121"
     CDS             complement(3326..4534)
                     /locus_tag="ACHA6B_001121"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8948710.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4646..4978)
                     /locus_tag="ACHA6B_001122"
     CDS             complement(4646..4978)
                     /locus_tag="ACHA6B_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8948711.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5126..6001
                     /locus_tag="ACHA6B_001123"
     CDS             5126..6001
                     /locus_tag="ACHA6B_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8948712.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6083..6664)
                     /locus_tag="ACHA6B_001124"
     CDS             complement(6083..6664)
                     /locus_tag="ACHA6B_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8948713.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6670..7929)
                     /locus_tag="ACHA6B_001125"
     CDS             complement(6670..7929)
                     /locus_tag="ACHA6B_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8948714.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7931..8977)
                     /locus_tag="ACHA6B_001126"
     CDS             complement(7931..8977)
                     /locus_tag="ACHA6B_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948715.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9212..9487)
                     /locus_tag="ACHA6B_001127"
     CDS             complement(9212..9487)
                     /locus_tag="ACHA6B_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8948716.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9730..11040)
                     /gene="der"
                     /locus_tag="ACHA6B_001128"
     CDS             complement(9730..11040)
                     /gene="der"
                     /locus_tag="ACHA6B_001128"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8948717.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11222..12451)
                     /gene="rpsA"
                     /locus_tag="ACHA6B_001129"
     CDS             complement(11222..12451)
                     /gene="rpsA"
                     /locus_tag="ACHA6B_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8948718.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12586..13266)
                     /gene="cmk"
                     /locus_tag="ACHA6B_001130"
     CDS             complement(12586..13266)
                     /gene="cmk"
                     /locus_tag="ACHA6B_001130"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8948719.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13315..13950)
                     /locus_tag="ACHA6B_001131"
     CDS             complement(13315..13950)
                     /locus_tag="ACHA6B_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8948720.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(14016..15419)
                     /locus_tag="ACHA6B_001132"
     CDS             complement(14016..15419)
                     /locus_tag="ACHA6B_001132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8948721.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(15406..16437)
                     /locus_tag="ACHA6B_001133"
     CDS             complement(15406..16437)
                     /locus_tag="ACHA6B_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948722.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16468..16689
                     /locus_tag="ACHA6B_001134"
     CDS             16468..16689
                     /locus_tag="ACHA6B_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8948723.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16854..17591)
                     /locus_tag="ACHA6B_001135"
     CDS             complement(16854..17591)
                     /locus_tag="ACHA6B_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8948724.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17744..19084)
                     /locus_tag="ACHA6B_001136"
     CDS             complement(17744..19084)
                     /locus_tag="ACHA6B_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8948725.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19321..20844)
                     /locus_tag="ACHA6B_001137"
     CDS             complement(19321..20844)
                     /locus_tag="ACHA6B_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8948726.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(21222..21833)
                     /locus_tag="ACHA6B_001138"
     CDS             complement(21222..21833)
                     /locus_tag="ACHA6B_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8948727.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(21956..22081)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(22163..22879)
                     /locus_tag="ACHA6B_001139"
     CDS             complement(22163..22879)
                     /locus_tag="ACHA6B_001139"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8948728.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(22885..23454)
                     /gene="scpB"
                     /locus_tag="ACHA6B_001140"
     CDS             complement(22885..23454)
                     /gene="scpB"
                     /locus_tag="ACHA6B_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8948729.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(23438..24208)
                     /locus_tag="ACHA6B_001141"
     CDS             complement(23438..24208)
                     /locus_tag="ACHA6B_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8948730.1"
                     /translation="MNKLTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(24205..24576)
                     /locus_tag="ACHA6B_001142"
     CDS             complement(24205..24576)
                     /locus_tag="ACHA6B_001142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8948731.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(24595..25482)
                     /gene="xerD"
                     /locus_tag="ACHA6B_001143"
     CDS             complement(24595..25482)
                     /gene="xerD"
                     /locus_tag="ACHA6B_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8948732.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(25578..26051)
                     /locus_tag="ACHA6B_001144"
     CDS             complement(25578..26051)
                     /locus_tag="ACHA6B_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8948733.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(26156..27019)
                     /locus_tag="ACHA6B_001145"
     CDS             complement(26156..27019)
                     /locus_tag="ACHA6B_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8948734.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(27215..29314)
                     /locus_tag="ACHA6B_001146"
     CDS             complement(27215..29314)
                     /locus_tag="ACHA6B_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8948735.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         8194 a         6263 c         4953 g        10062 t
ORIGIN      
        1 actcgttttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt
       61 gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt
      121 cgtgattttt tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct
      181 tagcattttt ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc
      241 cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc
      301 atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata
      361 tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct
      421 tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa
      481 ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa
      541 acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa
      601 tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc
      661 atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc
      721 ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac
      781 ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt
      841 ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc
      901 cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa
      961 aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat
     1021 ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa aaagactgat
     1081 agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag
     1141 accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc
     1201 taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga
     1261 caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat
     1321 acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc
     1381 tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt
     1441 ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc
     1501 cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat
     1561 cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc
     1621 tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac
     1681 tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac
     1741 acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc
     1801 aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc
     1861 tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc
     1921 gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg
     1981 agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa
     2041 tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag
     2101 catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt
     2161 ttggttcccc tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt
     2221 cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg
     2281 acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc
     2341 aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt
     2401 ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata
     2461 aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa
     2521 gataaaagtt tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta
     2581 tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa
     2641 cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt
     2701 ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg
     2761 gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat
     2821 tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat
     2881 ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc
     2941 ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga
     3001 tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc
     3061 caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc
     3121 aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga
     3181 ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg
     3241 gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag
     3301 ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa
     3361 cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag
     3421 cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta
     3481 ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca
     3541 tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc
     3601 ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga
     3661 caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac
     3721 gaatctcatt ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt
     3781 ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca
     3841 aaccgggaca gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt
     3901 catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat attttaccta
     3961 atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt
     4021 gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag
     4081 gattgcctac agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga
     4141 tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat
     4201 cttctgccaa cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg
     4261 attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc
     4321 catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg
     4381 cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc
     4441 cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct
     4501 tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct
     4561 tgtcctgaga taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt
     4621 caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc
     4681 tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat
     4741 tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg
     4801 gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac
     4861 ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt
     4921 gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct
     4981 ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga
     5041 gaaaatcgaa tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa
     5101 tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt
     5161 tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca
     5221 tttagcagaa gggggagtct cagggatcac cttgatctta agagctttat ttggtattga
     5281 tccagcttat acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct
     5341 cggtaaacgt tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg
     5401 gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct
     5461 acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac
     5521 aggcggcagt gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg
     5581 ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa
     5641 acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt
     5701 agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc
     5761 accactactg atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt
     5821 ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc
     5881 taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga
     5941 agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga aaaaactata
     6001 agaaaaaaga caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt
     6061 tttagtacta tactattttt aactaattgt tatgttcact tacccggtta cttttctttt
     6121 gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat
     6181 ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt
     6241 ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc
     6301 aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt
     6361 cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct
     6421 ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag
     6481 atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat
     6541 tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc
     6601 taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta
     6661 gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg
     6721 ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct
     6781 taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg
     6841 ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt
     6901 ccattctgca taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc
     6961 atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc
     7021 tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa
     7081 agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc
     7141 ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata
     7201 tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc
     7261 ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt
     7321 cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat
     7381 acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct
     7441 tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa
     7501 ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa
     7561 ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa
     7621 gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc
     7681 gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg
     7741 gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag
     7801 caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa
     7861 aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt
     7921 tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc
     7981 tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa
     8041 caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg
     8101 aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt
     8161 ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta
     8221 aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt
     8281 caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt
     8341 cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat
     8401 acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt
     8461 caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg
     8521 gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg
     8581 ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg
     8641 ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga
     8701 taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg
     8761 ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa
     8821 ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga
     8881 ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt
     8941 tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct cttcttattt
     9001 ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact
     9061 tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg
     9121 gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag
     9181 ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac
     9241 ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt
     9301 tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa
     9361 ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag
     9421 ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt
     9481 ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa
     9541 actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat
     9601 cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt
     9661 agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg
     9721 gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa
     9781 tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc
     9841 attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt
     9901 caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc
     9961 attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc
    10021 aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc
    10081 aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt
    10141 ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta
    10201 acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac
    10261 gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc
    10321 gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact
    10381 ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat
    10441 tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc
    10501 aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt
    10561 cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg
    10621 atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac
    10681 tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc
    10741 atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc
    10801 aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat
    10861 tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg
    10921 atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt
    10981 aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat
    11041 attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt
    11101 caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt
    11161 ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca
    11221 attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta
    11281 aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt
    11341 ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat
    11401 gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt
    11461 catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc
    11521 cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata
    11581 ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga
    11641 ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat
    11701 cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat
    11761 ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa
    11821 cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt
    11881 ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga
    11941 tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat
    12001 gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc
    12061 ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga
    12121 tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt
    12181 ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta
    12241 cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa
    12301 ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct
    12361 taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt
    12421 tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc
    12481 cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt
    12541 tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata
    12601 accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt
    12661 cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc
    12721 ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt
    12781 ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat
    12841 tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc
    12901 accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc
    12961 agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt
    13021 tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt
    13081 gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata
    13141 agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa
    13201 tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg
    13261 tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt
    13321 aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc
    13381 aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc
    13441 ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt
    13501 gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc
    13561 tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat
    13621 tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact
    13681 ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc
    13741 atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa
    13801 aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga
    13861 aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc
    13921 tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta
    13981 ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat
    14041 aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt
    14101 ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt
    14161 ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga
    14221 agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa
    14281 ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag
    14341 ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa
    14401 agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca
    14461 ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct
    14521 ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat accaaatgca
    14581 ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt
    14641 tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga
    14701 tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt
    14761 tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata
    14821 ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt
    14881 atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct
    14941 aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga
    15001 gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct
    15061 aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga
    15121 tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt
    15181 ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac
    15241 acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc
    15301 gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct
    15361 tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt
    15421 ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa
    15481 ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc
    15541 caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa
    15601 ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat
    15661 ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa
    15721 attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat
    15781 ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt
    15841 gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata attggttagc
    15901 taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc
    15961 agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa
    16021 agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga
    16081 aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg
    16141 gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg
    16201 cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa
    16261 aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc
    16321 gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag
    16381 agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt
    16441 attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc
    16501 tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa
    16561 ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa
    16621 gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat
    16681 aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata
    16741 agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc
    16801 tatctatagt taattgacta gttttttcac ggaccctata ggacccattt atattatttc
    16861 aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa
    16921 atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg
    16981 ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct
    17041 tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct
    17101 gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg
    17161 tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg
    17221 atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc
    17281 actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct
    17341 tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa aataagtaat
    17401 ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt
    17461 ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag
    17521 taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta
    17581 aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat
    17641 gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga
    17701 cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac
    17761 gatacacagc ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt
    17821 cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact
    17881 ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag
    17941 taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata
    18001 atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta
    18061 atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt
    18121 ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt
    18181 catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta
    18241 agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag
    18301 cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt
    18361 caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct
    18421 tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag
    18481 aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa
    18541 ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat
    18601 atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg
    18661 tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag
    18721 gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga
    18781 atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga
    18841 taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt
    18901 taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca
    18961 cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc
    19021 ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt
    19081 tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc
    19141 tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac
    19201 aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg
    19261 atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt
    19321 ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa
    19381 tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat
    19441 tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt
    19501 gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt
    19561 tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa
    19621 tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa
    19681 gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac
    19741 tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga
    19801 ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata acatagtaat
    19861 ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac
    19921 cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt
    19981 tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac
    20041 atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt
    20101 catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga
    20161 tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg
    20221 aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa
    20281 aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc
    20341 tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat
    20401 cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg
    20461 aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag
    20521 tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat
    20581 tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt
    20641 gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga
    20701 tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt
    20761 aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa
    20821 ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat
    20881 tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt
    20941 tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt
    21001 taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc
    21061 ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca
    21121 aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt
    21181 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
    21241 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
    21301 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
    21361 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
    21421 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
    21481 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
    21541 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
    21601 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
    21661 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
    21721 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
    21781 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
    21841 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
    21901 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
    21961 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
    22021 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
    22081 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
    22141 aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
    22201 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
    22261 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
    22321 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
    22381 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
    22441 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
    22501 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
    22561 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
    22621 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
    22681 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
    22741 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
    22801 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
    22861 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
    22921 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
    22981 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
    23041 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
    23101 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
    23161 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
    23221 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
    23281 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
    23341 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
    23401 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
    23461 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
    23521 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
    23581 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
    23641 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
    23701 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
    23761 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
    23821 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
    23881 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
    23941 attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
    24001 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
    24061 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
    24121 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
    24181 taatattgat ctccgtcaac ttattcactc tttcttcttt ttctttgcta gccattttcc
    24241 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
    24301 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
    24361 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
    24421 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
    24481 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
    24541 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
    24601 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
    24661 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
    24721 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
    24781 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
    24841 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
    24901 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
    24961 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
    25021 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
    25081 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
    25141 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
    25201 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
    25261 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
    25321 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
    25381 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
    25441 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
    25501 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
    25561 aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata gcattcctga
    25621 cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact
    25681 acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag
    25741 catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat
    25801 ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga
    25861 taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct
    25921 gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt
    25981 gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca
    26041 ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa
    26101 cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat
    26161 tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa
    26221 gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt
    26281 ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta
    26341 aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca
    26401 gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt
    26461 cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg
    26521 tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg
    26581 ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt
    26641 cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg
    26701 tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga
    26761 caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaaccgtaa tgaccgatac
    26821 ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc
    26881 cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc
    26941 cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag
    27001 ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt
    27061 ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg
    27121 aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa
    27181 cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc
    27241 atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt
    27301 tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc ccattttcga
    27361 ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag
    27421 caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa
    27481 tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg
    27541 aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg
    27601 cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg
    27661 cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt
    27721 tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct actgcttctt
    27781 ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta
    27841 tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg
    27901 cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat
    27961 ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc
    28021 actcattcac tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta
    28081 actctgaata tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat
    28141 cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa
    28201 gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt
    28261 ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg
    28321 ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt
    28381 tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg
    28441 cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag
    28501 taccctcttt gcttacagat tgattttcag catcggattc actatttatt tcatcgggct
    28561 ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat
    28621 tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg
    28681 tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt
    28741 tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga
    28801 tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat
    28861 aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc
    28921 ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct
    28981 catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag
    29041 gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta
    29101 aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat
    29161 cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta
    29221 tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca
    29281 tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag
    29341 gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg
    29401 atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt
    29461 agtctcaagc tt
//
LOCUS       DAYYHB010000026        29217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000026 DAYYHB010000000
VERSION     DAYYHB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.026"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..796)
                     /locus_tag="ACHA6B_001147"
     CDS             complement(293..796)
                     /locus_tag="ACHA6B_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8948736.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1155..2180
                     /locus_tag="ACHA6B_001148"
     CDS             1155..2180
                     /locus_tag="ACHA6B_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8948737.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2503..3381
                     /locus_tag="ACHA6B_001149"
     CDS             2503..3381
                     /locus_tag="ACHA6B_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8948738.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3857..3973
                     /locus_tag="ACHA6B_001150"
     CDS             3857..3973
                     /locus_tag="ACHA6B_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8948739.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4901..5239
                     /locus_tag="ACHA6B_001151"
     CDS             4901..5239
                     /locus_tag="ACHA6B_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948740.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5333..5563
                     /locus_tag="ACHA6B_001152"
     CDS             5333..5563
                     /locus_tag="ACHA6B_001152"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8948741.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5963..6334
                     /locus_tag="ACHA6B_001153"
     CDS             5963..6334
                     /locus_tag="ACHA6B_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8948742.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6608..7300)
                     /locus_tag="ACHA6B_001154"
     CDS             complement(6608..7300)
                     /locus_tag="ACHA6B_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8948743.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7303..8088)
                     /locus_tag="ACHA6B_001155"
     CDS             complement(7303..8088)
                     /locus_tag="ACHA6B_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8948744.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8325..9725
                     /gene="lacG"
                     /locus_tag="ACHA6B_001156"
     CDS             8325..9725
                     /gene="lacG"
                     /locus_tag="ACHA6B_001156"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8948745.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9750..10067
                     /locus_tag="ACHA6B_001157"
     CDS             9750..10067
                     /locus_tag="ACHA6B_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8948746.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10080..11753
                     /locus_tag="ACHA6B_001158"
     CDS             10080..11753
                     /locus_tag="ACHA6B_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8948747.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11767..13398
                     /locus_tag="ACHA6B_001159"
     CDS             11767..13398
                     /locus_tag="ACHA6B_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8948748.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13889..15454)
                     /gene="guaA"
                     /locus_tag="ACHA6B_001160"
     CDS             complement(13889..15454)
                     /gene="guaA"
                     /locus_tag="ACHA6B_001160"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8948749.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15513..16436)
                     /gene="coaA"
                     /locus_tag="ACHA6B_001161"
     CDS             complement(15513..16436)
                     /gene="coaA"
                     /locus_tag="ACHA6B_001161"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8948750.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16632..17237
                     /locus_tag="ACHA6B_001162"
     CDS             16632..17237
                     /locus_tag="ACHA6B_001162"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8948751.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17545..18561
                     /locus_tag="ACHA6B_001163"
     CDS             17545..18561
                     /locus_tag="ACHA6B_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8948752.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18702..20003
                     /locus_tag="ACHA6B_001164"
     CDS             18702..20003
                     /locus_tag="ACHA6B_001164"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8948753.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20049..20711
                     /gene="deoC"
                     /locus_tag="ACHA6B_001165"
     CDS             20049..20711
                     /gene="deoC"
                     /locus_tag="ACHA6B_001165"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8948754.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20732..21130
                     /locus_tag="ACHA6B_001166"
     CDS             20732..21130
                     /locus_tag="ACHA6B_001166"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8948755.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21196..22278
                     /locus_tag="ACHA6B_001167"
     CDS             21196..22278
                     /locus_tag="ACHA6B_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8948756.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22490..24052
                     /locus_tag="ACHA6B_001168"
     CDS             22490..24052
                     /locus_tag="ACHA6B_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948757.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24045..25178
                     /locus_tag="ACHA6B_001169"
     CDS             24045..25178
                     /locus_tag="ACHA6B_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948758.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25178..26134
                     /locus_tag="ACHA6B_001170"
     CDS             25178..26134
                     /locus_tag="ACHA6B_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948759.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26233..27399
                     /gene="deoB"
                     /locus_tag="ACHA6B_001171"
     CDS             26233..27399
                     /gene="deoB"
                     /locus_tag="ACHA6B_001171"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8948760.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27423..28241
                     /locus_tag="ACHA6B_001172"
     CDS             27423..28241
                     /locus_tag="ACHA6B_001172"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8948761.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28258..28965
                     /gene="deoD"
                     /locus_tag="ACHA6B_001173"
     CDS             28258..28965
                     /gene="deoD"
                     /locus_tag="ACHA6B_001173"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8948762.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         9174 a         5089 c         6303 g         8651 t
ORIGIN      
        1 ttatagaaaa ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt
       61 gagatattga ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt
      121 ttgttaaata aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata
      181 atatagtaga ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg
      241 tcatagatac acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa
      301 gacggacagt tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc
      361 ccaaaataat ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat
      421 aggcccataa acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta
      481 cgccaatacc gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc
      541 ctaatttttt agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa
      601 aaaagacaat caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag
      661 tagaaaaagg gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa
      721 atgatagctt gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt
      781 ttgatacata ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga
      841 aaaatcataa aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag
      901 cttttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa
      961 agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc
     1021 caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag
     1081 gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc caattactag
     1141 gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt
     1201 ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc
     1261 atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa
     1321 acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg
     1381 ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt
     1441 tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct
     1501 tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac
     1561 cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta
     1621 caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat
     1681 ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt
     1741 ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg
     1801 catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga
     1861 ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt
     1921 tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa
     1981 attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc
     2041 gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt
     2101 tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg
     2161 ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt
     2221 tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt
     2281 cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt
     2341 ttgtttatga tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc
     2401 ttacagatat tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta
     2461 tgtttatacc atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg
     2521 aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta
     2581 actcttccca aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag
     2641 gtgtttgagc aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag
     2701 aaaatagaca aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca
     2761 gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg
     2821 gcaacaatga atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat
     2881 cgctttgacc gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt
     2941 gaattggagt acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga
     3001 aagcagtgcc tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga
     3061 gatagttacg aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa
     3121 gtgatgtaca cagatacttg gttactatca cccaatctta ctaaatggtt gaaaaaagag
     3181 tcaaaaatct gtctttttgc agccgattat cgattattat cagttgatga acaggacgat
     3241 agtggagtcc cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt
     3301 acgagtttac agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc
     3361 ttaggtattc ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa
     3421 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
     3481 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
     3541 cttgtttctt ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac
     3601 acagtacgtt caataggtaa actagctata acaatagata ttaaagttaa cggacaagag
     3661 ggcctttttt aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa
     3721 tcagctagtc tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt
     3781 taaaacacaa atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag
     3841 aaagtgggtc gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag
     3901 cccaaatatc aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag
     3961 tgggaaaagg taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc
     4021 tattttcaat aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt
     4081 tttaacatat gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc
     4141 agttatatag ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag
     4201 ttatttttca attccctttc aacctcttat tttgaccagt agtattgatc actttacctt
     4261 ttaacacctt tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa
     4321 tcaaaggttt tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc
     4381 aaataaccat tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc
     4441 tcactccatt tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa
     4501 aagttaactt taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta
     4561 ttgctagaat aacatcaagg aataataagt tttggttttc agataatagt cgataagttg
     4621 tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact
     4681 attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg
     4741 aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa
     4801 tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt
     4861 agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag
     4921 tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat
     4981 tgttccggag actccattag ctagtgagcc aattactgat attgatggaa accaaatagg
     5041 atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt
     5101 aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga
     5161 tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa
     5221 gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat
     5281 ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt
     5341 agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa
     5401 tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta
     5461 cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc
     5521 tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga
     5581 aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa
     5641 ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa
     5701 gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct
     5761 taattagctt attgattcta taatattttt gatttatata ggaaattaaa aatattatag
     5821 aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat
     5881 ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt
     5941 tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat
     6001 caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat
     6061 tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa
     6121 agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct
     6181 taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga
     6241 tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca
     6301 gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt
     6361 atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac
     6421 tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa
     6481 gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc
     6541 actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt
     6601 gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat
     6661 ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga
     6721 atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt
     6781 aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa
     6841 gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc
     6901 agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag
     6961 tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa
     7021 gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt
     7081 ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt
     7141 tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac
     7201 catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca
     7261 aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt
     7321 attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc
     7381 ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata
     7441 aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt
     7501 tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg
     7561 aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt
     7621 gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc
     7681 tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat
     7741 tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat
     7801 cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat
     7861 ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg
     7921 agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg
     7981 aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt
     8041 tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc
     8101 taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta
     8161 aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt
     8221 atgattattt gtatttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag
     8281 ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag
     8341 aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag
     8401 gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg
     8461 atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg
     8521 gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga
     8581 aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa
     8641 acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg
     8701 gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc
     8761 atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca
     8821 atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg
     8881 cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg
     8941 aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg
     9001 cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag
     9061 atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg
     9121 aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat
     9181 ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg
     9241 atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag
     9301 tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc
     9361 tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt
     9421 acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca
     9481 ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg
     9541 cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct
     9601 catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga
     9661 agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta
     9721 tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt
     9781 ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag
     9841 taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa
     9901 cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg
     9961 agatgggatt catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag
    10021 atctgattca agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta
    10081 tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta
    10141 atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt
    10201 caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat
    10261 ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg
    10321 tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca
    10381 aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg
    10441 tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt
    10501 cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta
    10561 ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc
    10621 ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc
    10681 aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg
    10741 ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc
    10801 atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca
    10861 atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga
    10921 attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac
    10981 tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt
    11041 tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta
    11101 ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat
    11161 tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg
    11221 ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg
    11281 acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag
    11341 cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag
    11401 tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac
    11461 tttgtgctgg cgcagggaca agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg
    11521 aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc
    11581 aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag
    11641 aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat
    11701 taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga
    11761 gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg
    11821 gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca
    11881 gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt
    11941 tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg
    12001 tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa
    12061 acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt
    12121 cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt
    12181 ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa
    12241 atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga
    12301 atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct
    12361 gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta
    12421 atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc
    12481 acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt
    12541 tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg
    12601 atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct
    12661 tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag
    12721 tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc
    12781 ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg
    12841 aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat
    12901 tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat
    12961 ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa
    13021 acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt
    13081 ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat
    13141 gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag
    13201 tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt
    13261 gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt
    13321 tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt
    13381 tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc
    13441 cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc
    13501 agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt
    13561 tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag
    13621 ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata
    13681 tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa
    13741 cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga
    13801 accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg
    13861 gattccccct ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag
    13921 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
    13981 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
    14041 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
    14101 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
    14161 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
    14221 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
    14281 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
    14341 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
    14401 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
    14461 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
    14521 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
    14581 ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac
    14641 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
    14701 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
    14761 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
    14821 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
    14881 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
    14941 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
    15001 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
    15061 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
    15121 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
    15181 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
    15241 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
    15301 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
    15361 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
    15421 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
    15481 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
    15541 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
    15601 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
    15661 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
    15721 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
    15781 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
    15841 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
    15901 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
    15961 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
    16021 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
    16081 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
    16141 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
    16201 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
    16261 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
    16321 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
    16381 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
    16441 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
    16501 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
    16561 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
    16621 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
    16681 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
    16741 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
    16801 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
    16861 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
    16921 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
    16981 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
    17041 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
    17101 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
    17161 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
    17221 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
    17281 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
    17341 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
    17401 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
    17461 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
    17521 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
    17581 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
    17641 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
    17701 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
    17761 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
    17821 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
    17881 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
    17941 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
    18001 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
    18061 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
    18121 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
    18181 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
    18241 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
    18301 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
    18361 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
    18421 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
    18481 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
    18541 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
    18601 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
    18661 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
    18721 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
    18781 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
    18841 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
    18901 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
    18961 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
    19021 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
    19081 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
    19141 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
    19201 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
    19261 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
    19321 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
    19381 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
    19441 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
    19501 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
    19561 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
    19621 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
    19681 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
    19741 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
    19801 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
    19861 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
    19921 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
    19981 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    20041 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    20101 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    20161 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    20221 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    20281 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    20341 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    20401 atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc
    20461 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    20521 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    20581 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    20641 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    20701 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    20761 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    20821 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    20881 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    20941 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    21001 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    21061 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    21121 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    21181 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    21241 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    21301 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    21361 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    21421 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    21481 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    21541 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    21601 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    21661 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    21721 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    21781 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    21841 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    21901 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    21961 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    22021 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    22081 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    22141 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    22201 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    22261 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    22321 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    22381 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    22441 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    22501 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    22561 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    22621 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    22681 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    22741 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    22801 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    22861 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    22921 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    22981 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    23041 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    23101 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    23161 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    23221 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    23281 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    23341 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    23401 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    23461 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    23521 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    23581 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    23641 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    23701 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    23761 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    23821 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    23881 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    23941 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    24001 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    24061 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    24121 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    24181 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    24241 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    24301 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    24361 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    24421 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    24481 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    24541 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    24601 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    24661 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    24721 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    24781 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    24841 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    24901 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    24961 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    25021 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    25081 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    25141 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    25201 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    25261 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    25321 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    25381 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    25441 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    25501 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    25561 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    25621 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    25681 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    25741 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    25801 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    25861 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    25921 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    25981 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    26041 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    26101 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    26161 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    26221 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    26281 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    26341 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    26401 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    26461 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    26521 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    26581 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    26641 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    26701 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    26761 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    26821 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    26881 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    26941 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    27001 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    27061 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    27121 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    27181 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    27241 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    27301 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    27361 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    27421 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    27481 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    27541 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    27601 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    27661 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    27721 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    27781 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    27841 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    27901 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    27961 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    28021 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    28081 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    28141 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    28201 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    28261 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    28321 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    28381 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    28441 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    28501 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    28561 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    28621 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    28681 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    28741 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    28801 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    28861 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    28921 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    28981 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    29041 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    29101 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    29161 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaa
//
LOCUS       DAYYHB010000027        29018 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000027 DAYYHB010000000
VERSION     DAYYHB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.027"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..1227)
                     /locus_tag="ACHA6B_001174"
     CDS             complement(124..1227)
                     /locus_tag="ACHA6B_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8948763.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1449..1919)
                     /locus_tag="ACHA6B_001175"
     CDS             complement(1449..1919)
                     /locus_tag="ACHA6B_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8948764.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2176..3495
                     /locus_tag="ACHA6B_001176"
     CDS             2176..3495
                     /locus_tag="ACHA6B_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8948765.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3936..5576
                     /locus_tag="ACHA6B_001177"
     CDS             3936..5576
                     /locus_tag="ACHA6B_001177"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8948766.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5730..7052
                     /locus_tag="ACHA6B_001178"
     CDS             5730..7052
                     /locus_tag="ACHA6B_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8948767.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7114..7545)
                     /locus_tag="ACHA6B_001179"
     CDS             complement(7114..7545)
                     /locus_tag="ACHA6B_001179"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8948768.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7609..8445)
                     /locus_tag="ACHA6B_001180"
     CDS             complement(7609..8445)
                     /locus_tag="ACHA6B_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8948769.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8635..8937)
                     /locus_tag="ACHA6B_001181"
     CDS             complement(8635..8937)
                     /locus_tag="ACHA6B_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8948770.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8963..9394)
                     /gene="ruvX"
                     /locus_tag="ACHA6B_001182"
     CDS             complement(8963..9394)
                     /gene="ruvX"
                     /locus_tag="ACHA6B_001182"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8948771.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9391..9657)
                     /locus_tag="ACHA6B_001183"
     CDS             complement(9391..9657)
                     /locus_tag="ACHA6B_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8948772.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9911..10636)
                     /locus_tag="ACHA6B_001184"
     CDS             complement(9911..10636)
                     /locus_tag="ACHA6B_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8948773.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(11002..11072)
                     /locus_tag="ACHA6B_001185"
     tRNA            complement(11002..11072)
                     /locus_tag="ACHA6B_001185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11038..11040),aa:Gly,seq:ccc)
     gene            11189..11941
                     /locus_tag="ACHA6B_001186"
     CDS             11189..11941
                     /locus_tag="ACHA6B_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8948774.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11957..12436
                     /locus_tag="ACHA6B_001187"
     CDS             11957..12436
                     /locus_tag="ACHA6B_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8948775.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(12687..14267)
                     /locus_tag="ACHA6B_001188"
     CDS             complement(12687..14267)
                     /locus_tag="ACHA6B_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8948776.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(14260..15003)
                     /locus_tag="ACHA6B_001189"
     CDS             complement(14260..15003)
                     /locus_tag="ACHA6B_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948777.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(15179..15955)
                     /locus_tag="ACHA6B_001190"
     CDS             complement(15179..15955)
                     /locus_tag="ACHA6B_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8948778.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(16203..17039)
                     /locus_tag="ACHA6B_001191"
     CDS             complement(16203..17039)
                     /locus_tag="ACHA6B_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8948779.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(17050..17979)
                     /locus_tag="ACHA6B_001192"
     CDS             complement(17050..17979)
                     /locus_tag="ACHA6B_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8948780.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            18171..18524
                     /locus_tag="ACHA6B_001193"
     CDS             18171..18524
                     /locus_tag="ACHA6B_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8948781.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(18598..19254)
                     /locus_tag="ACHA6B_001194"
     CDS             complement(18598..19254)
                     /locus_tag="ACHA6B_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8948782.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            19480..21414
                     /locus_tag="ACHA6B_001195"
     CDS             19480..21414
                     /locus_tag="ACHA6B_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8948783.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(21500..22723)
                     /locus_tag="ACHA6B_001196"
     CDS             complement(21500..22723)
                     /locus_tag="ACHA6B_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8948784.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(22717..24588)
                     /locus_tag="ACHA6B_001197"
     CDS             complement(22717..24588)
                     /locus_tag="ACHA6B_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8948785.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(24609..25727)
                     /locus_tag="ACHA6B_001198"
     CDS             complement(24609..25727)
                     /locus_tag="ACHA6B_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8948786.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(25810..26643)
                     /locus_tag="ACHA6B_001199"
     CDS             complement(25810..26643)
                     /locus_tag="ACHA6B_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948787.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(26636..27553)
                     /locus_tag="ACHA6B_001200"
     CDS             complement(26636..27553)
                     /locus_tag="ACHA6B_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8948788.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(27576..27755)
                     /locus_tag="ACHA6B_001201"
     CDS             complement(27576..27755)
                     /locus_tag="ACHA6B_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948789.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(27780..28904)
                     /locus_tag="ACHA6B_001202"
     CDS             complement(27780..28904)
                     /locus_tag="ACHA6B_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8948790.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         8895 a         5838 c         4941 g         9344 t
ORIGIN      
        1 tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt
       61 gaactataaa aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttgt
      121 ttcttaaata aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt
      181 tccagctaat tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac
      241 tcctgtactt cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc
      301 tgcacctgat gcgattgctt tgaaaacatg ctgtccgcgt cgtacaccgc tgtcaaacac
      361 gattggtaca cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgctgctgg
      421 accaccatct agttgacggc caccatgatt agatacccag attccgccag caccagattc
      481 taatgaacga tagacatctt cttctgactg gacacctttc acatagactg gcaaatcaga
      541 atgagcagca ataaattcta catcttttgg actaagcttc tgtttggaag atttgtacac
      601 agcatccatc gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagtgg
      661 gaaggtaaac ccatttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt
      721 caatacgatt gcttttgctc cattacgctt ggccacttca agaatatcca aattgatccc
      781 atcatctttg ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct
      841 tatctcttct aatgtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc
      901 tacacctttt gcagaagctt tctccgcctt cacatgagct aagccatgag cagcaactgg
      961 tgccatgatg attggtgcag tcagcgtttc tttgtcaaaa tgtgtagttg tatcgggtaa
     1021 ttctacatct cttaatacat gaggaataat cagctgatga ttgaatgccc gttcattttc
     1081 ttgataagta aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat
     1141 cacttgtgaa gcggctattt ctaaatcttc catattgata aaatccacta ttccttcatt
     1201 tgtacctgct tgatacgttt tttccatttc aatgccttcc ctctataaaa aattgaatga
     1261 ataatatgat agcgtttaca cactttatat tactcttccg tattgataca ggcaaataat
     1321 ctgtttacat ttcagtcatt ccagttgtta ctactctgaa agaaatgttt agaacacaaa
     1381 tagataaaaa acgatgggat tttgacaaaa gtcgtgtcaa agtccctttt acgaaatact
     1441 gtggatgcct attgaaataa gtgcgggttg tttctttgta tttcaatcgt ttcttgccat
     1501 tcttcgttta attttggatt accgttttct tccttgaacg cataaggtcc caatccaaaa
     1561 agctcagtta gtatttgatt cacgattcca tatagctcgg tgtgatcgga cacatgatta
     1621 ttgaagatca tctcattatc accacgtttg aatctcacat tcgggctctc atgatgaacc
     1681 tctatgcagt tttccccttt ttttaactga cggatttctg aaaacatctc ataaatatct
     1741 tctccaataa aaccttcagc aagcgccaga ctgtccacaa gaccgcctac tgtcaactca
     1801 gagattatcg ctttgttggc accaagcttt aatgtatagc ttgttgcttc tactacgccg
     1861 catgcattgc aaccaccttc tgatagttcg ctttttctga tcaattgaac ttctttcatt
     1921 ttctgttcct cctcttagtt ttctacttat gtatttttta tcacctgacg taagtgtatt
     1981 ttatcgtcac gatcacgtga tttattcttc agagattcta cctgaataat tcatactttt
     2041 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
     2101 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
     2161 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
     2221 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
     2281 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
     2341 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
     2401 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
     2461 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
     2521 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
     2581 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
     2641 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
     2701 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
     2761 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
     2821 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
     2881 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
     2941 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
     3001 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     3061 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     3121 ctatcagaaa atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg
     3181 gagaacttca ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc
     3241 tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc
     3301 ttaagaacga ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg
     3361 aagtttctga aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta
     3421 tctagttatc atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc
     3481 agtcaatgga tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga
     3541 agtatgctca aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg
     3601 tttgaagtaa ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat
     3661 aaaatgtgcc aaagaattaa agctatgaat tattcaggat tctatttatc tatctaacat
     3721 atcctttatt cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc
     3781 aaggattgtt gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac
     3841 tcatataaat aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat
     3901 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa
     3961 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa
     4021 tcactggtat gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg
     4081 gtcaatactt aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca
     4141 atacgaaccg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     4201 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     4261 cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa
     4321 acgcagcaga tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag
     4381 gtatgggtga ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca
     4441 ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta
     4501 ataacgaaga attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag
     4561 gcgaaactta ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag
     4621 aattgttatt acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat
     4681 acgaagataa aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag
     4741 ctggtgtatt aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga
     4801 catttggtgc tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc
     4861 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt
     4921 tattgtttga agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct
     4981 cagaattcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc
     5041 caacgatcat gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     5101 aaatggcggc tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg
     5161 cagaagcaaa agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag
     5221 gtatccctgc tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata
     5281 atgcgttgat gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa
     5341 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     5401 caggagcagc tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg
     5461 aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca
     5521 aagaagctgt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt
     5581 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     5641 ggcaaaacag cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata
     5701 cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag
     5761 ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc
     5821 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga
     5881 atatgattgg cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa
     5941 caaaaatccc tatagtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct
     6001 ctgccgcgat tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact
     6061 ttgtcagcaa tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc
     6121 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
     6181 tttcaatggt cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt
     6241 ttgcaaattc cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag
     6301 ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt
     6361 cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag
     6421 ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag
     6481 gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg
     6541 gtttgatggt atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta
     6601 aagtccatgc ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat
     6661 taccagaata ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga
     6721 cagcagctgt tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg
     6781 ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt
     6841 caggatttgt cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct
     6901 tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga
     6961 tggaacttat tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca
     7021 tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat
     7081 cggcttgatg accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg
     7141 cattttatcg gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc
     7201 tgcagaatta agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat
     7261 caaggccttt ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt
     7321 gaacatcgga tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     7381 tttagaagta ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt
     7441 cttcatttgt ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat
     7501 ttctcgtagt gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca
     7561 atttttattt ttttaaatga atttttttat cacagtatac tccttttttc acttaaaaaa
     7621 agacttttca gtgattttga cttgcttgaa tccagatcct tctaatgttt gcaagatgac
     7681 gaaagcattg taaagattga tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt
     7741 cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc
     7801 agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc
     7861 aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc
     7921 ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc
     7981 aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca
     8041 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     8101 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     8161 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     8221 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     8281 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     8341 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     8401 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     8461 tcttgtttat atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca
     8521 aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaaaaccg
     8581 ggctctactt ttcagagagt ccagcttttc ttacacctta tcgattcttt tgttctattc
     8641 ttcttccgcc atgaatgtat tgaatacttc ttcgatcata tcccattctg cgtctgtttc
     8701 gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa
     8761 ttctacatct tcatcttctg aaacaccagc tggatataaa agaacatagt tacgtccaaa
     8821 ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat gtttcatttc cttggtcatc
     8881 caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag
     8941 attacctctt ttctttttat tattaaaata attgcttacc tgagccgtct aaataatttt
     9001 gtaaaatcat cacagctgct aatttatcga tgactttttt tcgtttcgct cttgaagtat
     9061 ttgcctgctc tactagcata cgttctgcct gaacagtggt tagtcgttca tcttgataat
     9121 caaccggcaa ggagaatagt tctttgatct tctctccata tgacatggaa gcttctgccc
     9181 ttggtccgat ggaattgttc atattcttag gtaatccaac tacaaaacgg tctacttcgt
     9241 actgcgccac taattccttc aaccgatcaa aaccaaactc ttcttctgcc tcattgattc
     9301 gaatgatttc tactccttga gctgtccagc caaagggatc gctcacagca acgcctactg
     9361 tcttagaacc gacatccaag cccattactc tcataaattc actccatgat ttgagaggta
     9421 gtctttgaca attacttcca tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc
     9481 atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt
     9541 gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac
     9601 ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat
     9661 tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaccctta ttttactcga
     9721 agaaagggaa aactacaaac aaatattcca aacttctgaa taaattaaga aacacgattt
     9781 gtctgatttt tccttttttc tgtggctttt tcaaaagccc tctttccata aagtatacgc
     9841 tatatttctt tgaaagtatg tttttacaca attttttcat aaagttcata aaaaattcga
     9901 attgctgcta ctaatttttc gttttcgttg cttgccctgt ctcataattg atggtgtagc
     9961 catcttgaac attgtagata aagacattga attcaatttt tttgtcttca atgctttctg
    10021 cttccaactg cacacctctt gctaccagtt catttccctc gaaatgagga gtgacacgat
    10081 agcgtacatg atgatttgtt tccttgatat atgccgcaat atcattttcg tgggcaagca
    10141 tgtacggagc attcaacgaa cgtgtccctg tcattaagtt tttaatgtta ttattttgac
    10201 cagtcaactg gaaaccaatc aaatggctgc ggttgtataa ccattgtccg tcacttaatt
    10261 tcttttgttt ccaacctgtc ggatcaatat acaaaggttc tctcttttca gtaggaaaca
    10321 agtcttttcc caacatcgca ttggcttgtc ctactctgtt caatttgtct atatttgaat
    10381 agctttccca tgtccctttg gaaagactaa gatcctcttt tgtaaactcc ggctgattcc
    10441 cattgatttc tatttcttga tgttttccat catattctgg cacatccgct acttgagaag
    10501 cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa tctttttgtg
    10561 tggtggaaat accgagtgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg
    10621 ttgttttttt gctcatttct ttactccttt actaacagtt ccctaaatct taacatatgt
    10681 tcccctataa ataaatactg gaaagaaata gaaacagtaa aaaaataaaa ccagttttta
    10741 gacggcttaa gaatacaaaa aaagagacgg attcatcgtc tctactcaat cacaaaacac
    10801 tatttacaag gtatttatta tcattcaaaa aatttttctt aatactcaaa actaaacggc
    10861 ttctgtacga cctcttggta aacagtgttc aaaagcttgc atctgcggta ataagtccaa
    10921 ccaagtcaaa aacataagaa gcttttttgc ttgactattc ttattacttg atgcagggca
    10981 gctttttcac agcctctttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg
    11041 aagctgggat tttaccacta aactacaccc gcgatacaaa aacagtatac gcctctttca
    11101 ctccttcctc aagttttttt attctttacg cttccgatat gttttgtagt aaaataaaac
    11161 taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga
    11221 cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga
    11281 agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac
    11341 tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac
    11401 caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt
    11461 tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg
    11521 ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat
    11581 ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg
    11641 atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt
    11701 atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa
    11761 tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa
    11821 cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc
    11881 gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta
    11941 ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac
    12001 tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc
    12061 tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt
    12121 tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt
    12181 gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt
    12241 agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc
    12301 ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt
    12361 taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa
    12421 acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct
    12481 tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag
    12541 taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt
    12601 gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac
    12661 atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa
    12721 taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac
    12781 caaaatggat aaaacaaagc caacattccg tataagacga taagaataat actgatgatc
    12841 aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg
    12901 ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac
    12961 aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc
    13021 tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat
    13081 ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac
    13141 agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt
    13201 aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca
    13261 gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag
    13321 gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta
    13381 aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg
    13441 tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa
    13501 aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc
    13561 tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca
    13621 ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc
    13681 gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga
    13741 gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac
    13801 aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa
    13861 ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc
    13921 tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt
    13981 ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa
    14041 agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca
    14101 aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata
    14161 gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca
    14221 tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc
    14281 tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc
    14341 attcaataat tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag
    14401 tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc
    14461 ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc
    14521 ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat
    14581 cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat
    14641 atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt ctaattcata
    14701 ggcagctgaa atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa tatctgggga
    14761 atcaggcaca taaccgattt tttgtttgat actcaatcga ttttccgtca atttcattcc
    14821 atctactaaa atcgttcccg atgatggttc aataatacta actaaacttt taatcgtagt
    14881 tgattttccg gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag
    14941 agacaagtct tttaatgctt gttttttccc gtaactttta cttacattga tgaattcaat
    15001 catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt attggtggag
    15061 taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg ctacttgggt
    15121 tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt tgttttcttt
    15181 aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca atacttctct
    15241 ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact ctgctaatag
    15301 atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa tactcatatt
    15361 cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca tctttcaata
    15421 ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt tcatcgtaat
    15481 ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt
    15541 gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt
    15601 cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta
    15661 ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa
    15721 gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa
    15781 tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc
    15841 cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta
    15901 ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa
    15961 gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt
    16021 taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa
    16081 tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa
    16141 agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc
    16201 ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta
    16261 acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct
    16321 tctattttat accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa
    16381 ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct
    16441 gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg
    16501 aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc
    16561 ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct
    16621 gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc
    16681 cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg
    16741 agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg
    16801 attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct
    16861 tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa
    16921 atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca
    16981 gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata
    17041 ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag
    17101 cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac
    17161 cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga
    17221 agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat
    17281 gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata
    17341 agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa
    17401 ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc
    17461 attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga
    17521 cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac
    17581 aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa
    17641 tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc
    17701 aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc
    17761 gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg
    17821 attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga
    17881 gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt
    17941 actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc
    18001 tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat
    18061 ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat
    18121 taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa
    18181 ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc
    18241 tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca
    18301 cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta
    18361 aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc
    18421 ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag
    18481 aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga
    18541 aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta
    18601 ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg
    18661 caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc
    18721 agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg
    18781 tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc
    18841 tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc
    18901 attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag
    18961 taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt
    19021 taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc
    19081 taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc
    19141 acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg
    19201 ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg
    19261 acctccacgt attgtctaat tacatctttg atttctccat catagcagta tttataggaa
    19321 aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt tatttatcat
    19381 ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt
    19441 ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat
    19501 cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt
    19561 acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag
    19621 atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact
    19681 ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg
    19741 ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc
    19801 attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg
    19861 ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta
    19921 ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc
    19981 ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt
    20041 ctagacgaac caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg
    20101 caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta
    20161 gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg
    20221 aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag
    20281 caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc
    20341 acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct
    20401 caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta
    20461 gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc
    20521 aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa
    20581 tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta
    20641 gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag
    20701 acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt
    20761 gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt
    20821 ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg
    20881 attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca
    20941 ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg
    21001 atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat
    21061 gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat
    21121 gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag
    21181 caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg
    21241 atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat
    21301 cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca
    21361 aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa
    21421 aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa
    21481 aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag
    21541 cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct
    21601 tattataaac agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt
    21661 tcaattgtcg cataattttc caaacagcag actggtataa gttcttttct gttttgtcta
    21721 atagacctga ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat
    21781 ctttttcttc tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg
    21841 gcaaccgatt cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt
    21901 tcaaataacg ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac
    21961 aggaattatc actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat
    22021 ccatataaaa tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt
    22081 gaatatgagc ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat
    22141 acaataatgg aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac
    22201 taggatctcc ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt
    22261 ctgcagcatc aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta
    22321 cggttgataa agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa
    22381 tcctgtggat aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg
    22441 tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt
    22501 ctcttaatgg ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac
    22561 tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt
    22621 ctttttccat taattcttta taacattcat ttatccattt ttctggatgt attatctctt
    22681 caaacatcac tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt
    22741 attgttttta ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt
    22801 gttccatact tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca
    22861 atgggttcct tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat
    22921 caagtttgta ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact
    22981 taaacagata atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc
    23041 tatgattatt ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac
    23101 atattctgta tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag
    23161 ccaagttctg atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt
    23221 acatacaatg tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc
    23281 attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat
    23341 tgccatttct gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata
    23401 agcaaatttt aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc
    23461 attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag
    23521 tttagttcct tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc
    23581 aggtagcgcc aacaataaaa aaatcttatt cagccataat ggagataacg tactattata
    23641 gggttcagtc aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc
    23701 aaatatttct ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc
    23761 taatttaaaa ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa
    23821 acgataaatc atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat
    23881 atagtctcta gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg
    23941 ttctagtacg ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg
    24001 gcgacctctt gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc
    24061 aaatgcttct ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc
    24121 gacaattatc ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga
    24181 gaaccaatga ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc
    24241 atgatgatat aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg
    24301 tatttctccc caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat
    24361 taatttcaac catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg
    24421 agagaatcct tccattcctg caattttatt tccatatcct accccttgat gtatataatc
    24481 aatttgtgcg ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa
    24541 gtcaaagtaa tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc
    24601 tctttttact accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa
    24661 aaataatcag aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct
    24721 ccgcctccaa tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt
    24781 tggagtatat tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct
    24841 gctaattcca gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct
    24901 tttacagcaa aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt
    24961 actgctatct tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac
    25021 gcgaatccat ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct
    25081 tgcccgccaa gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg
    25141 gttgatccat ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt
    25201 ttagagaacc tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc
    25261 atactatcaa taatcatcca accaggttta tggtcattcc atgctactaa atagttccct
    25321 gaaatattat agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt
    25381 ttgttttgag actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca
    25441 tgatgaacgt ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct
    25501 gcctctttga acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa
    25561 aaaccattgg tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg
    25621 taagaaatat atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa
    25681 gctacattca aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca
    25741 ttcttttaat ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg
    25801 ttattttttt tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca
    25861 cacataaatc aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata
    25921 attaattggc tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata
    25981 tttatcttta tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa
    26041 gataagtaaa ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa
    26101 aatcacccac tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg
    26161 actgctatat tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac
    26221 acctgcataa gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt
    26281 ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat
    26341 aaaacgatac tttgctaatg cataacccgc cattgtagaa ctggctgtag ctgaaactgt
    26401 actgattaca gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat
    26461 gatggcttgc cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa
    26521 aatttcttga ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat
    26581 taaggcacca aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt
    26641 catttttatc acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca
    26701 tcgtccaggc aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata
    26761 aattgactcc tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat
    26821 ttgggatatt gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga
    26881 ttggctttag catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta
    26941 tttcagcact ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag
    27001 tgttatatcc tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag
    27061 gatttgttaa ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc
    27121 cagtcggatt gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg
    27181 gaatataata gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa
    27241 cagcaaagta aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa
    27301 cattcgtgta agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc
    27361 caacaaattc aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg
    27421 gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt
    27481 ttttatgaga cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg
    27541 aagattgcat catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg
    27601 tacttatgag caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc
    27661 caatagttat atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa
    27721 acagtatttg atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct
    27781 tagtagtcaa actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc
    27841 acgcccagct ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat
    27901 tgttgtgcca cttgtggatt tttggcatct ttagggacat aaaaacttga tcctcctgat
    27961 actgcacctt ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg
    28021 tcagatgcat caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac
    28081 attgcagatt cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct
    28141 gagtcgatca tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg
    28201 atatttcctt cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat
    28261 tcttggtccc cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct
    28321 tttgccacct ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca
    28381 gcaaattttt ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata
    28441 atcgtttcat tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg
    28501 ccatatttgt cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc
    28561 agcacacctg ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt
    28621 accaattttg gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt
    28681 aagtcaatat ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt
    28741 gtcagtttgc cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct
    28801 gcgatgtttg ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg
    28861 gtaatgttta gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc
    28921 ttttcaagtt cttatataaa acgctttcac ttatgtatat taacggaatt agtaacacaa
    28981 atatttatat aaaaaaatat tttacttgtc gttttggc
//
LOCUS       DAYYHB010000028        28261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000028 DAYYHB010000000
VERSION     DAYYHB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.028"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..925)
                     /locus_tag="ACHA6B_001203"
     CDS             complement(29..925)
                     /locus_tag="ACHA6B_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8948791.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(918..1598)
                     /locus_tag="ACHA6B_001204"
     CDS             complement(918..1598)
                     /locus_tag="ACHA6B_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8948792.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1599..2381)
                     /locus_tag="ACHA6B_001205"
     CDS             complement(1599..2381)
                     /locus_tag="ACHA6B_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8948793.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2374..3291)
                     /locus_tag="ACHA6B_001206"
     CDS             complement(2374..3291)
                     /locus_tag="ACHA6B_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8948794.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3455..3709)
                     /locus_tag="ACHA6B_001207"
     CDS             complement(3455..3709)
                     /locus_tag="ACHA6B_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948795.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4566..7793
                     /gene="fss3"
                     /locus_tag="ACHA6B_001208"
     CDS             4566..7793
                     /gene="fss3"
                     /locus_tag="ACHA6B_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8948796.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7906..8220
                     /locus_tag="ACHA6B_001209"
     CDS             7906..8220
                     /locus_tag="ACHA6B_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8948797.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8233..8607
                     /locus_tag="ACHA6B_001210"
     CDS             8233..8607
                     /locus_tag="ACHA6B_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8948798.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8617..8952
                     /locus_tag="ACHA6B_001211"
     CDS             8617..8952
                     /locus_tag="ACHA6B_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8948799.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9035..10375
                     /locus_tag="ACHA6B_001212"
     CDS             9035..10375
                     /locus_tag="ACHA6B_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8948800.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10453..11127
                     /locus_tag="ACHA6B_001213"
     CDS             10453..11127
                     /locus_tag="ACHA6B_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8948801.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11120..11284
                     /locus_tag="ACHA6B_001214"
     CDS             11120..11284
                     /locus_tag="ACHA6B_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948802.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11360..12502
                     /gene="dcm"
                     /locus_tag="ACHA6B_001215"
                     /pseudo
     CDS             11360..12502
                     /gene="dcm"
                     /locus_tag="ACHA6B_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12492..12782
                     /locus_tag="ACHA6B_001216"
     CDS             12492..12782
                     /locus_tag="ACHA6B_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948803.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13041..14225
                     /gene="mobT"
                     /locus_tag="ACHA6B_001217"
     CDS             13041..14225
                     /gene="mobT"
                     /locus_tag="ACHA6B_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8948804.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14222..14359
                     /locus_tag="ACHA6B_001218"
     CDS             14222..14359
                     /locus_tag="ACHA6B_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8948805.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15105..17015
                     /gene="ltrA"
                     /locus_tag="ACHA6B_001219"
     CDS             15105..17015
                     /gene="ltrA"
                     /locus_tag="ACHA6B_001219"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8948806.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17119..17343
                     /locus_tag="ACHA6B_001220"
     CDS             17119..17343
                     /locus_tag="ACHA6B_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948807.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17356..17859
                     /locus_tag="ACHA6B_001221"
     CDS             17356..17859
                     /locus_tag="ACHA6B_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8948808.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17919..18308
                     /locus_tag="ACHA6B_001222"
     CDS             17919..18308
                     /locus_tag="ACHA6B_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8948809.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18295..20742
                     /locus_tag="ACHA6B_001223"
     CDS             18295..20742
                     /locus_tag="ACHA6B_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8948810.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20747..22870
                     /locus_tag="ACHA6B_001224"
     CDS             20747..22870
                     /locus_tag="ACHA6B_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8948811.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22867..23871
                     /locus_tag="ACHA6B_001225"
     CDS             22867..23871
                     /locus_tag="ACHA6B_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8948812.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23890..24801
                     /locus_tag="ACHA6B_001226"
     CDS             23890..24801
                     /locus_tag="ACHA6B_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8948813.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            24988..25244
                     /locus_tag="ACHA6B_001227"
                     /pseudo
     CDS             24988..25244
                     /locus_tag="ACHA6B_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25461..25676)
                     /locus_tag="ACHA6B_001228"
     CDS             complement(25461..25676)
                     /locus_tag="ACHA6B_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948814.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(25925..26143)
                     /locus_tag="ACHA6B_001229"
                     /pseudo
     CDS             complement(25925..26143)
                     /locus_tag="ACHA6B_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(26438..27877)
                     /locus_tag="ACHA6B_001230"
     CDS             complement(26438..27877)
                     /locus_tag="ACHA6B_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8948815.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9209 a         5066 c         5915 g         8071 t
ORIGIN      
        1 tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg gagatggaga
       61 agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg
      121 acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa
      181 aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga ttggccactg
      241 ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac
      301 gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata
      361 cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt
      421 cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca
      481 tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta
      541 atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta
      601 cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag
      661 aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt
      721 gttcattttc atccaaccat gtattcagtg catctactag gtccattaga ttcttttctc
      781 gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct
      841 tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa
      901 cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt atgaatatac
      961 acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc
     1021 tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact
     1081 tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca
     1141 gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag
     1201 atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt
     1261 cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc
     1321 aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc
     1381 gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct
     1441 agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa
     1501 gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg aacagcttca
     1561 tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg
     1621 aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt
     1681 ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc
     1741 gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaatgaag
     1801 gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg
     1861 aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa tacaggaaat
     1921 acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca
     1981 agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa
     2041 agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta
     2101 ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa
     2161 gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc accgttgatg
     2221 actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc
     2281 caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc
     2341 ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa
     2401 ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca caggaacttt
     2461 tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat
     2521 aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc
     2581 ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc
     2641 tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag tggcgatctg
     2701 tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa
     2761 acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc
     2821 atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg
     2881 catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt
     2941 cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt
     3001 atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg
     3061 tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat
     3121 caaacccata atagttctca taaaggtaga cttacccgct ccattttccc caatcaatcc
     3181 atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg cagcaccgta
     3241 ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct
     3301 tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc cagaaatgta
     3361 taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca atacgtgtct
     3421 ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct ttcttttcga
     3481 ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc agatcaagct
     3541 tgagtaattt taccagaccg gttataagga taactatagc aagactccct cccagaaaaa
     3601 ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg
     3661 ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata agtcgtcctt
     3721 gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta
     3781 atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac
     3841 caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc
     3901 tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt
     3961 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat
     4021 aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata
     4081 gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt
     4141 ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac
     4201 gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag
     4261 ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaaac
     4321 cttgatttat aaaggttttc ccgtaaatat attttattat atttgattaa atttgaaatg
     4381 tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt agattaaatt
     4441 tttaggctca ttcattctca aatccattct attcagtcaa taaaaatttt aaaaagactt
     4501 tataaacaaa gatgttcaag cgagcatctt atttttttgt gcttaatttg aaaggagaac
     4561 atttcatgta taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg
     4621 ttcaagcaat tgttggagca attgctccta cgattacttt tgctgatgaa attacccatc
     4681 cacaaacggt aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta
     4741 gtgatggtcg tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcttta
     4801 aacaagatat tttctgtatt gaaccaggag tcccgattta caatgagttc actcctggct
     4861 atgagaaaaa cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga
     4921 aaaaagtagg tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag
     4981 ttaatgggta tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag
     5041 ccattgaggc aaaaataaat caagccattg acgattatca gaagaaacca accttccata
     5101 atagtacggc taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga
     5161 atttgtcaga gttcgatgag gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg
     5221 gtaatcagtt gataatcacc ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta
     5281 agaaatctgc tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa
     5341 tggccggggc gattgataaa cctaatacct acacaatcaa aattgatgtg gaaacggagg
     5401 gttctttaaa gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca ggaacggttt
     5461 tccatttaga ctttggaaaa actttacctg caaaagacgt gactactgac aaagaaggca
     5521 ttgctacatt ggatgagatt cctcatggta caaaagtaac cattaccgaa aaatcagtac
     5581 cggcgcctta tacgattgat accaccccca tgactaccac cattaaagca ggcgaaacca
     5641 tttacgtgac ttcaaaaaac gcacgggaaa aagggcaaat cattctggat aaaagcggag
     5701 ttgaaacagg gagtgatctt tggaatgaca attattcctt agctggtaat acgtttgcca
     5761 ttcgcaaaga cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc
     5821 atgcggaaac accaaaagag attgctaatg cgttggaatt gggaacctat tatgtgaccg
     5881 aaactaaagc tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt
     5941 atgccaatca aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat caagaagtca
     6001 ctggcgaaac cactttgacg aaagaagaca aagatactgg ggataaagca caaggtaagg
     6061 cagtctttga gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa gctgttaaat
     6121 ggagtgaggc ttttaaacca gagttagcga agggaacgaa aggttctgat gaaacggtca
     6181 ctttgacttt agacgaaaag aaccaagttg ccgttaaaca tttagctatc aacgagtatt
     6241 attgccaaga aacaaaagcg cccgaaggat atagccttga tgaaacaaag tatcctgtat
     6301 ccatcaaaaa ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa
     6361 aagaacaagt gattcgcttt ggctttgatt tctttaaatt tgctggatca gctgcgggca
     6421 ctaccgaaac tggcttcaat gatctgacct ttaaagtgtc gccattggaa gggaccaatg
     6481 aaatcacagg tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg
     6541 gttatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc
     6601 cagaaggttt tcaaaagatt acgccattag aaatccgttc tacttttaag gaaaacaaag
     6661 aagactttgt gaagagtgag tatgtcttta ccattaccga acaagaccaa aaacaaccaa
     6721 ttaaaacggt gaccgttcct tacgagaagc tgacgaacaa agcattttct gttagtttga
     6781 accgtttaat gctctatgat ttgcctgagg aagaagatag tttaacttct cttgcgactt
     6841 ggaaagacgg aaacaaagaa ttaacttctc tggattctac cgagctagtt gataaattga
     6901 gttacaatct acatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg
     6961 atgcgacaaa agctgcccaa gaaaaagatg aaaaagccaa accagtggtg attgcagaaa
     7021 caagcgcaac gttggcgaac aaagagaaaa caggaacttg gaaaattcag cataaattaa
     7081 ccgccgaaca agttttaaat aaaactattg tcttattcaa ctatgtgtat gaaaataagg
     7141 aagcttttga agcaggcgat aagccagtag cgaaggatgt tagcttgaat aaccaagccc
     7201 aaactgtcag ttgtacagtg gaacaccatg tttctatcca aacaaaggcc cacctagaaa
     7261 acggttctca gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta
     7321 ctcatgatgt actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac
     7381 cggatggtac aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata
     7441 aagaattcac caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa
     7501 ccaagtttac ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca
     7561 atgaagattt gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa
     7621 gtacgccgaa acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca
     7681 cagtgaagac cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt
     7741 ttactttgat ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg
     7801 agatgataca agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg
     7861 cgctattttt gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt
     7921 gtcgtgccag atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt
     7981 ataacagaag gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc
     8041 agtaagcaac gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat
     8101 tttgatcaag accaaccgct aaaagccgtg aatccccatt tggttgccaa aggctatgag
     8161 attgagaatc gtgggtttac tgattacgtg ttgtatgttg atgatttagt gaaagcatag
     8221 gaggaaaaga agatgagatt aacagaaggt attgtcgtag attcaggttt aacatttggg
     8281 aagttgcgtt tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacaatt
     8341 ggtaatgagg taaaagaacg aacctataat ttaaaatcct ctgcacaagg gcgaatgatt
     8401 caagttagta tcccagctaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag
     8461 ttggtcaatc caatcgtgga taccgttgcc aattatgttt ttcgagaagg aactaccgtt
     8521 aattggttca ttaaggcgga tgatttagtc ttgaagcggc agccgaatca aggaaatcct
     8581 acgaaccaaa atgaaagaaa gaaataggtg aagattatgg aaattaatgt tgaacatatt
     8641 ttagattgtc tcgatcaata tggaaaaggc gagctgactg aggaacaact cgttaaagca
     8701 ttaacctctg atgaaaaaat gtttctgatt atacatcaag gtttacttgg agaaaatagc
     8761 gacagtgaag aagactctga tgtactagac tggttaggag aagaagagtc tttctttata
     8821 gttgtcgaag taaatgaaaa cttatgtcgg caggcagaat ccattttgga agaaattggt
     8881 gtggagatgc cggatgctat tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc
     8941 ccctcctcgt agcagtaaat gactagcata actttttgaa attgcctaat gaaatcctat
     9001 tgggcttttt cgattggagg tggaaaccat caaaatgtac aaaggacatc gcatacgcgc
     9061 aggagatcag cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg
     9121 ttggatgagc gtcttttatt ttcaagagtt tagacaattt gatatttcta agctatcact
     9181 ttctactatt gaaatcgttt ggtctataaa agatttagta tgtctgcttg gaagtctcgc
     9241 tttttcagga gctatgatat tgctttatat tcactttttt cttgatcatt ggcgaagctt
     9301 gtggcatcga caaaagctag ctcggatgat tctagagaac cattggtatg aagtgaaaca
     9361 aactcaaagt gaaggctttt tcaaagattt gaatagtagc cgaaccagag agactatcag
     9421 ttacttcccc aaaatctgtt atcgaatgaa agatggatta ctgtctattc gcgttcaaat
     9481 ttcacttggg aaataccaag atcagctttt aaaattggaa aagaagttag aaagcggact
     9541 gtattgtgag ttagtagaaa aggaactcaa agactcctat gtggagtata ctttactgta
     9601 tgatatgatc gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg ggactctgcg
     9661 attgatgaaa aatcaagtat gggcttatga ttctttaccc catatgttaa tcgctggtgg
     9721 cacgggtgga gggaagacct atttcctcct caccattatc gaagcactat tgaagtcaga
     9781 tgcagaattg tttatcctcg atcccaaaaa tgcagattta gctgatttag gtacggtgat
     9841 gcctcatgtt tattcacaaa aggaagaaat ttctgcttgt gtggaagatt tctatgaacg
     9901 tatgatggct cgtagcaagg caatgaaaga aatgtctaac tacaaaacag gagagaatta
     9961 tgcctatctt ggacttccac ccaacttttt aatctttgat gaatacgtgg cttatatgga
    10021 gatgttaacg acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt
    10081 gggtcgtcaa tctggtttct ttctgatttt ggcttgccaa aggccggatg cgaaatattt
    10141 aggagacgga atccgcgatc aattcaactt tcgcgtggct ttgggtcgta tgagtgaact
    10201 cggatattcc atgatgtttg gggaggtcga aaaaaacttc tttatgaagc acatcaaagg
    10261 tcgaggttat gtggatacag gtggcagtgt gattagtgag ttttatacgc cacttgtacc
    10321 aaaagggtat gatttcttag attcaattag taaattagaa aagatgaatg attgagtgat
    10381 cttcaatact gactttatac ttcaagtaac gtataatact tttatatagt aggaagaaag
    10441 gggataatta atatggaaag taatgattct tatttagagt atgcaaaagc aaataaggat
    10501 aagtttattt cttatattat caaagggaaa tcccccgaag ttggacgaga cgcagttttt
    10561 atggcaggga gtcctggggc tggaaaatca gaggttgcca ttggattagc tgagaattat
    10621 gataaccatg tgattattga tgcggattac tttcgcaccc agtttcctga ttataatgga
    10681 aaaaactcta gtctctttca aaaagcatct tcttggttgg tagagcaggc atttagatat
    10741 gtcttagatc atggctattc ttttattctg gatgcaactt ttgcaatatt aagtgctgaa
    10801 aaaaatatct cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta tgtttatcaa
    10861 gaccctaaaa tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg
    10921 ccgaaaaagg catttattaa cgcttttttc aaatcaagag agaacattgc aaaagtaaag
    10981 agtaaacatc cagatgtatt gcttcatatc ataataaaag attatacgaa taatatttcc
    11041 gaagttcatt atgctacgga taatctagaa ttatttttac cactagggta tagtaggaaa
    11101 gatttggagg ttcaattaca tgactgagaa agaacaagta acgaaaattg taaagaaata
    11161 caataagagc attgcggatt tatcggaaaa tgctactgca aaagagttca aaacggtgat
    11221 taaatatgta gccgatcagg ccaatgaaaa acaacggaaa ttagttggat taaataaaaa
    11281 atagtaggac aaattaataa tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt
    11341 gcaatgaatg aggtaatgta tgagattttt agatttattc gccggtattg gtggctttcg
    11401 tttaggtatg gaacaggcga gccatcattg tatcggtttt tgtgaaattg ataaatttgc
    11461 tagacgtagt tataaagcaa ttcatgatac aagtaaggag gtggaaatgc atgacatcac
    11521 aagtgtatca gatgagttta ttcaatccct cggaccagtg gacatccttt gcggcggatt
    11581 tccgtgccaa gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac
    11641 tttattcttt gaaatcgctc ggttcaccac tcttctccga cctaagcttt tattccttga
    11701 gaacgtccga ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac
    11761 gctggatgga ttggggtatg acgtggagtg gtaggtgctt aactcaaagg cctacattcc
    11821 ccaaagccgg gagcggatat tccttatcgg acattctcga gacgcatgta ccgaacaagt
    11881 atttcctatc attggaccgt cttcaacatc tgatcaaaac atcagaaatt tggtaaatat
    11941 caatccttcc aatcgaggaa tgggtggaca agtctatggt tcagatggcg tagcgccaac
    12001 cttaacaggt gatgaaggaa tcaaaatcgc actgccagca agggatggaa tctctgtagc
    12061 aggactatta ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc
    12121 ccccaccttg tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga
    12181 atatcaagag gtgaagccag aagacagtgt gaatctggct tttcctaatt ccacaagtcg
    12241 ccgaggtcgt ctgggaaaac agagtgttca taccttacta acaggagacc aacaagcgat
    12301 cgtcacggat cagtatcaaa ttcggaagct aacacctaga gagtgttggc gacttcaggg
    12361 ctttcccgat tgggcttttg atcgagattc tcaagtcaat tcagatagtc agttgtataa
    12421 gcaagccgga aactctgtaa cagttccggt catttttgat attgccagaa gattaaaaga
    12481 gttggatgta catggtttat gatgagttga ttggagaaat ttattgggtg attggaaaga
    12541 ttcagtcaga cccagaatta gaagaacaat tgcgacggtt aaaatttgat attcgcaaga
    12601 atggtgtcaa agtcgctggc gatccatatt taatgaatga ggagacggat gctcgaattg
    12661 aaataaatca agtaattgca gaatttgagc ggattgcgga ccaagcaaaa gaaccagata
    12721 ttcgtcaata cttgttagaa atgaaagcag agttggaagt aaatggtatt acagatgagt
    12781 aacctaaacc aacacaactg actgttaggc gctgaccgcg gaggattcgc gaagcagaaa
    12841 tcctccgtgg tgctgtgcct gttcatggac ggcgttagcc gaccatgatg tctcaccccc
    12901 gtatctaaca ggggggtaca aaaacacaaa aaataagtaa gcaaccatta agaatgcttg
    12961 ttgtatcaaa gatagtaagt aatttgtata ggtgttaact catttgaaaa agttaacacc
    13021 ttgaatttgg aggtgagaac ttggcacaac ggaatttaga ttatcggcta ttaaaagacc
    13081 gacgcaacga atacggcgtt tcacaaaata aattggctac ggcttgtgga cttagtcggc
    13141 cgtacttaaa tcagatagaa aatggtggcg taaccgcatc tataaaaacc atgaggaaaa
    13201 tttttgacca actggaaagc tttaatcccg atttgccttt gacgttactg tttgactatg
    13261 taaggattcg ctttcccaca acggatgcac gaaaaattat tcaagaaatt ctccatttga
    13321 aatttgagta tatgctccat gaagattatg ccttttactc ctatcaagaa cagtatgtca
    13381 tgggtgatat tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt ttagaattaa
    13441 aggggcgagg ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt agctggtacg
    13501 actttttcga ggattgtcta aaggccggtg gcgtgatgaa acgcttggac ttggcaatca
    13561 atgatcgagt ggggctattg gatatacctg agctaacgaa aaaatgtcag aaggaagaat
    13621 gtatctcctt gtttcgtacc tttaaaagtt atcgttccgg ggaactttta aaggctgatg
    13681 aaaaggacgg catgggcaat accttatata tcggcagtct caagagtgag gtttatttct
    13741 gtttgtatga aaaagactat gaacaatata tcaagttagg gattccatta gaccaaactg
    13801 agacgaaaaa tcgttttgag attcgactga aaaatgaccg tgcttttcat gcgattcaag
    13861 atttgttaaa aggtcgtagc attgagagca ccacgttttc cattatcaat cgttacttgc
    13921 gatttgcgga taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg
    13981 ggcgttttat tggacggaat cggaaagaga ttcaattaac ttccgagcca aaaccgtata
    14041 ccattgaacg aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta
    14101 aggagttgga tcgattgaat cggacaacct atatccagga tatggtacag aatgcacgct
    14161 tatcagaccg acataaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta
    14221 tatgaaaaga ggaatcgtca tgggaattgt aaaagatatt tttttggtat ttggtggcag
    14281 tatgcttggt gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat
    14341 ggaaaagaaa ggaaagtgac aagatgaatt tcggacaaaa tttatgtgcg actcgttcct
    14401 tagtgaaaag ctaaggcact aataaaagaa cgaaaaagtt tggttattag gagaactgat
    14461 aatctgacag gtggaatgat gaagtaacgc tctgaaacgc ctgccctgag cctccgacaa
    14521 gcattgtatg aggaaactca gaatgtttga agctcggtaa agacggctga aagataccgt
    14581 aatagttgat gaacatatat cttctatcga aaactatcca gaggtgggta gcgtatccaa
    14641 caggtcgggg gtctataaaa tatctatggt tagaatgttc tataaggaac tgacgaaaca
    14701 gcgaatgtac gggtctaaat ttgaactttg tagaaatgca aaggtacgag aaatcgtagc
    14761 tggtgtggca aagtaagaat ataggttatg aaatgccata taacgttaca ggcggtatca
    14821 agccagcagg cttatagcta gtgacctaag gatatatatg tatagataag attatcggaa
    14881 cgaggaaagg tattgggttc tcatcaagag aataaactga cgaagaacaa taagcagttg
    14941 aactaatgct gaaaagcaga gggcgaacct aagaaattct tgtaatgaga atggaggaat
    15001 gccctcaagt cgtgtatata ttagaaaagt attatgttgt ttcaataagg attacgagta
    15061 agataaaaaa gctcactcaa tttcaagtaa aggagtgaag ccttatgcct aaaaataaaa
    15121 atgaaacatt gtgtgtagaa gatttaagac acgctgaata ttacgaaatg caaaacacct
    15181 ttgatgattt atatgccaaa agtaagaatg gagatatttt cactcatctt atggatatta
    15241 tcctatcgag agaaaatata ttactggcat atcgaaatat caaagcaaat gcaggcagta
    15301 aaacagcagg aacagacggt acaataatta aagatattgg caaattgcca gcagaaacag
    15361 ttgtcaaaaa agtaagatac attgttgcag gcagtccaca tgggtatcga cctaagccag
    15421 tgaggcgaaa agaaatacca aaaccaaatg gtaaaaccag acctctaggt attccttgta
    15481 tgtgggacag gcttatacaa caatgtataa aacaagtatt agaaccaatc tgcgaagcta
    15541 aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat gcaataaaag
    15601 caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt gatattaagg
    15661 gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct atgggaatac
    15721 gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc aaaatgacta
    15781 acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata tccccactac
    15841 tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg caggaaaatc
    15901 ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt aaaagcaacg
    15961 cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt tatgcagatg
    16021 attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata gcaatcactc
    16081 aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga attgtaaatg
    16141 ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg agaagaaaga
    16201 agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa gacaagctgg
    16261 tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta gaagaaacaa
    16321 gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca acgaatgtaa
    16381 atctggattg tatgaaactg aatagagctg taatggctat cttcactaac agattaggta
    16441 caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag gaggcaacaa
    16501 gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct attttccctg
    16561 ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat tgctacactc
    16621 tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg atgtaccagt
    16681 taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga atttcgctat
    16741 tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa gctaatgaaa
    16801 tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat aaaaatctta
    16861 ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa acaatacaaa
    16921 aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat aaatatagag
    16981 tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat acttttcaat
    17041 ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga atggggggaa
    17101 aagtttgaga tatttataat atcaggaact tacctatcca tatatcaatg gtttttaacc
    17161 aatgcccaat ccttagtctt actggcaatt gtggtcattg gtttgttttt aggatttaag
    17221 cgggagtttt ccaaactgat tggctttttg gtgattgctt tgattgcggt cggattggtc
    17281 tttaatgctt ctggggtgaa ggatgtctta ctcagtttgt ttaaccgaat tgtcggtgcg
    17341 taagggggaa gcacgatgga acagatgcgt gtttatattg cgaacctcgg gaaatataac
    17401 gaaggtgaat tggtgggggc gtggtttacc ccacctgtcg attttgatga agtgaaagaa
    17461 cgaattgggt taaatgacga atacgaggaa tatgcaattc atgattatga acttcccttt
    17521 gagattgatg agtatacgcc aattgaggaa attaatcggt tgtgtggctt agcggaggaa
    17581 cttgaaggaa cgccagttgg tgaagttgct tctgagattc aacatgcttt cttcaattct
    17641 tttgaggaga tggtagagca tgtggatgat attatctatt atccagattg cgatgatatg
    17701 agtgatgtag catatttctt gattgtagaa gctggtgatt ttggagaagt accagaacac
    17761 ttgaagacgt atattgattt tgatgcgtgt ggtagagact tggagattgg aggcaattat
    17821 ttggtaacga gtcgaggtgt ttttgagtat ccatattaac aaaattattg aagggtagtt
    17881 gatttctaaa ctatccttct ttttaaagga atgatttgat gaagaaaata aaaagctaca
    17941 ccagtatttg ggcagttgaa aaggtcattt atgcaattaa tgactttcaa ctgccttttc
    18001 ctgtgacttt taaccagatg gcttggtttg ttttatcctt gctgtttgtc atcgtatttg
    18061 cccatgtgcc tccactatct atgatagagg gggccttttt aaaatactta ggtataccgg
    18121 tggcagttac ttggttcatg tcgcaaaaaa cgtttgacgg taagaaacca ttgggatttt
    18181 tacgatcttt tattagctat cacttacgat ttaaggtcac tttcgcagga aagaaagtca
    18241 aagaacagaa gaaacgatgg gacgaaccca ttactctggt aaggagtgtg aactatgtac
    18301 ccgattaagt atattgaaaa taatcttgtc tttaatcaag agggagaatg ttttgcctac
    18361 tatgagttaa taccttacaa ttactccttt ttgtcacctg aacagaaata tcaagtccac
    18421 gataactttc gtcaattgat tgcgcaaaat cgtgaaggca agattcatgc cttacaaatt
    18481 gccacagaaa gtagtattcg tgccacgcaa gaacgatcaa aaaaggaaat taccgggcga
    18541 ttaacagaag tagcgaaaca acgaattgat ttacagacag aggcattagt atccatgatt
    18601 ggcgatagtc agattgatta tcgctttttt attggcttca aactgattgc gacagatgaa
    18661 gaggtcaatc taaagaatct caagaaatct ttcttctccg gctttcaaga atttgtctat
    18721 ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga aattcgccga
    18781 tataccaagc ttgaaaaact aatggaaagt aaattggctc gaagatttaa agtcagacga
    18841 gtcacaccga gtgatctgat gtatttgatt gaacatatct atggcgaaaa gggtacacca
    18901 tttgaagaat atgaatttca gctaccaaag aaaaagctga agtcggaaac tttggtgaaa
    18961 cgttacgact tattacgtcc cagtcgttgt ttgattgaag aaaagccccg gtacttatgc
    19021 atggaacatg agaatcatga atcatatgta gcgtacttaa caattaatac aattgtgggg
    19081 gaaatggagt ttccctcctc agaacttttc tattaccagc aacaacaatt tacattccca
    19141 attgacacta gtatgaatgt ggagattgtg acgaacaaaa aggcacttgc taccgttcgc
    19201 aataagaaaa aggaattaaa agacttggac aaccatgcct atcaatctga caatgaaaca
    19261 aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac cttggatcag
    19321 tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga atcagtagat
    19381 gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa tgtaaaacta
    19441 gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc tagtaagcga
    19501 tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact tggttttggc
    19561 gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt agatacgggg
    19621 cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaaggattc agttaccaat
    19681 gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag taacaatctc
    19741 ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc aaaaggggaa
    19801 cggggtggct ggaaagaaac cttgccagaa attgcggatg aaatcaatat tctcaactta
    19861 actagtaagc caaaaaatca aggattactt gatccttatg tgattatgga gaaaaagaag
    19921 gattctgaaa gtctagcaat tgatattctg acctttttaa caggaatttc tagtcgagat
    19981 ggggagaagt ttccagtgtt gcgtaaggcc attcggacgg ttacccaaag caatcaacgg
    20041 ggattattac aagtaattaa tgagcttcga aaagaaggct caccagttgc ggaaaatatc
    20101 gcggatcaca tcgagtcctt taccgattat gattttgccc atttgctttt ctcagatggt
    20161 tcgattaccc agtcgattag tttagatagg cagttaaata tcattcaagt agcggatttg
    20221 gttttaccag atgcagaaac cacttttgaa gaatatacca ccatggaatt gcttagtgtt
    20281 tccatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa tcgagaaatc
    20341 ttcaaaattg tagacttaga tgaggcgtgg agttttcttc aagtagcgca agggaaaaca
    20401 ttatcgaata agctcgttcg tgctgggcgg gctatgaatg ccggggttta ctttgtcacg
    20461 caaaatagtg atgatttact ggatgaacgc ttgaagaata atatcgggtt gaagtttgcg
    20521 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg cttggatcga
    20581 gaagatgaaa gcaatcaaaa acgcctacga gatttggaaa atggccaatg cttgatgcag
    20641 gatttgtatg gtcgggtagg cgtggttcag gtacatccag tctttgaaga actcttccat
    20701 gcctttgata cccgtccacc tgttcaagag agaatggagt gaggggatga aaaagaaact
    20761 ttttcggatt gtgggaatcg tttttattag catactcact ctgatagttc tattgagttt
    20821 agttggtacg gtagccgagg cgacagggct agttgatgat acagttgaag caggaaatct
    20881 atattccaaa tattcactga acaactatca actagatttc tttgtggata gctcttggga
    20941 ttggttgcct tggaattggg gtgatggctt agggaaaagc gtgatgtatg gtctttatgc
    21001 cattactaat ttcatttgga cggtgagttt gtatttgtcg aacgccacgg gttatgtggt
    21061 tcaagaggct tacaaattag atttcatttc ggatacggct gaaagtattg gaaagaatat
    21121 ccaaacattg gctggtatta ctgaaaatgg actccagacc tccggatttt attttgggtt
    21181 tctattattg atgattttag ctctgggggt gtatgtggcg tataccggat tattaaaacg
    21241 agaaaccacg aaagcagttc gagccgtgat taattttgtg atgatttttc tactatcggg
    21301 ttcgttcatt gcttatgcgc ctacctatat cacaaaaatc aatgacttta gctcagatgt
    21361 tagtgaagcg gcccttaccc ttggaaccga gatcgttgta cccaattcag aaagccaagg
    21421 aaaagatagt gtggatttga ttcgggatag tttgttttcg attcaagtcc aacaaccttg
    21481 gttactactg caatttgatg attccaacat agaagaaatt ggtgaagacc gggtcaacaa
    21541 gattctatct gtgagtccag atgaaaacaa aggcaaagat cgggaagaag cggtcaaggc
    21601 agagattgaa gacaatgaca atgcgaatct cagtattacc aagaccatga accgtttagg
    21661 gaccgtggtc tttttggtat tgttcaatat tggcatttcc ttctttgttt ttcttctaac
    21721 agggattatg ttgttctcgc aaattctctt cattatcttt gcgatgtttc tgccaattag
    21781 tttcttattg tccatgttgc cgacttatga aagtttagga aagaaggcga ttattcgttt
    21841 attcaatacg attatgatgc gagcgggtgt tactttggtg attacgactg catttagcat
    21901 ctccacgatg tttttcaata tttcggcaac ctatccattt ttcatggttg cttttctgca
    21961 aattgtgacc tttgccggta tctatttcaa attgggcgac attatgagta tgttcaacct
    22021 ccagagtaat gatagccagt caatgggaag acgcgtcatg cgaaaaccgc aaatgttaat
    22081 gaatcgaaaa ttgcggcaac tcaatcgaaa tgtgggacga gctttggctt ttggtggtgg
    22141 tgcagcagtc ggaaataagt tggcaaagga acaatcaaaa tccaaattta agccatccgg
    22201 ttcttctcta cgaaagaatt cacgattacc gaacgatcac gaaatactat ctgacttaac
    22261 taaggagaat ccagtttcaa ataataagaa acaatcccga atgaatttgg ctgggagaaa
    22321 aatcggcaaa gttttggata cccaagcttt ggtgaaagat aagactaaac aagtaaagga
    22381 tcaggtaagg aatacgccaa cgaatttgaa atacaatctc cataaaggaa tcgaaaagtc
    22441 gaaaaaagca ccagaagaat tcaagcgcgg actgttccaa gaaaaagcgg atcgagtgga
    22501 tttgcgagaa aaacaacgac aacgaagaaa tgcgaaaatg gacgaaaaac gcaagatact
    22561 gagtgaagca acaaatcgtc agggaaaaag aagggcgaat attccaatag ataaagcagc
    22621 tcaacctcag aaagatatga tgctgaaaaa agaagcacca aagcggattg tgagagcaaa
    22681 tctgaatccc gaaatcaaac gaaaactaac tagtcaagaa atcatttcaa aagggaaaag
    22741 ccaatcagca agtaagaaaa attctttcca acaagtgaaa cagaggtcca ctctgccaaa
    22801 gcagaccaat caaaaagtac aaaaaagaat gaaccatcgt ccaaaatcaa ggtcaggtga
    22861 gaaaaaatga acttgaagaa agtgggtgtt atttcattaa cttttctgac catctctttt
    22921 atgggtattt tgatgtgtgt gggtttgttg tttggtgaag acagtgaggg aaatggtagt
    22981 tctggcactt ctcctggcgg aactagtgtc tcagaagaag tattgaaaca ccgtgcaatg
    23041 gtggaaaagt atgccaaaga atcggatata acagagtata ttccaattct tttggcgatt
    23101 attcaagtgg aatctggtgg cacaatggaa gacgttatgc aaagttctga gtcagccgga
    23161 ttaccaccta attcattaag taccgaggca tccatcaaac aaggctgtct ctattttgcg
    23221 tctttggtta agcgttccaa agaactaggg tgtgatcaag atagtattat tcaggcctat
    23281 aactatggtg gcgggtatct agatcatgtc gggaaaaacg ggaagaagca tagttttgct
    23341 ttagctgaat ctttttcaaa ggaaaaatca ggtggtcaaa aagtcgatta ccccaaccca
    23401 atcgcgatta aggaaaatgg tgggtggcgg tataattatg gcaatatgtt ttactgttta
    23461 ttagtgaagc aatacctaac tacaactcag tttgatgaca aaatagtcca agaaattttt
    23521 gatgaggcat ttaagtatga aggaacggcc tatgtgtttg gtggctcaag tccagaaacg
    23581 gggtttgatt gtagtggctt gacccaatgg tgttatgcca aagttgacct gaaacttcct
    23641 cgggtggctc aggatcaata tgatgccatg actcatattg atctaaaaga tgccaaacca
    23701 ggtgatttag ttttctttca ttcaacctat gatgccggta cttatgtgac ccatgtgggc
    23761 atttacgcag gggagaatcg catgtttcat gcaggagatc cagtggggtg gacgaatcta
    23821 acggaaaact attggcggca gcatcttatc ggagctggcc gatacaaata agagaaaaga
    23881 ggaaaaagaa tgaagataaa aattgaacgg aatcaaaagg aaaagagccc aaaaaagaaa
    23941 aaagaacgag ttgtatccgt gggtaagcat cgaaaaatgg tgcttgccct ttggctttta
    24001 ttgtcttgta gtcttgcttt tggcgtttat aaaaatttca ccgccattga tcaacataca
    24061 actcatgaaa aagtggttat aaaagagaag gtagtgaata cttctggcgt tgaaagtttt
    24121 acagaggatt ttgcgaaaga atatttttca tggaaaaata acaaagaagc aattgaaaag
    24181 agaatgagta atctcgaaca gtaccttaca gaaaacggtc tcgctttaat ccaagatatg
    24241 gttcgagcag atattcccac gagttccgaa gttcaatctg taaaaattct agatgttgaa
    24301 aaacactcgg aagaatttgt cgtttcattt ttagtggagc aacatattac agagggaaaa
    24361 aagacgcaat caatttcttc tgcatatcga gtgacaatct ttgaagatga aaacaggaat
    24421 catttagtaa ctagtttgcc aacaatgatt ggaaagccag acaaggcaaa atatgaagca
    24481 aaacaagtag aaaacgattc agaaattgat gccaaaacaa cggaagaaat caccgatttt
    24541 ttagaaacat tttttaaact ttatccatca gcttctgaaa aagaattgga gtattatgtt
    24601 gaaaatggtg cgatgcgacc aatcaatatt gacttaaagt tttcggagtt agagaatcca
    24661 gtttatctac aaaacggaaa aaattttcta gtgagtacaa agataaaata ccttgataat
    24721 gtatcaaaaa ccactagcta ctttcagtac aaattagtat tacaaaaaaa tggaaactgg
    24781 aaaatcgttg atgttaaata ataatgactc aatctaattt tattagaagt tataagtagt
    24841 gtaacttatt tcaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccattcgg
    24901 taactcgaaa accgacaatt aagacatagt gcctttttat tttaatccat gtcataatca
    24961 gtctatatag gaaatggttg atataccatg gaattagata caaataatca ttcagttttt
    25021 cttcttggtt acccccttat tttagtagtg aaacattgta aacatgtcat tgatgatgta
    25081 atgtcagctt atgcaaaaac tgcctttgaa agaatttcag aatcacatca cattacctta
    25141 gatgaatgaa atcgtgataa agaatacttt catattatgc tcaaagcaca acctaaaacc
    25201 gaattaacaa aattcagcaa tgcctaagct cacgtctgat aaaatgagac tttctaaggg
    25261 tcaaaaattt cctttgaata cgattattgc ttagataaaa gtaaaagcct tagaactacg
    25321 gggatagctt ggttaataag agatatcgtt gttagtaaag acataggctt gcaagtatgc
    25381 tctattccca agaatctccc ccttcaagca ccaaggatcg ttaaattcag ctaagtggca
    25441 agtaattcac tagacgataa ttatctccat atttcaatct cagcttttcc atttttcata
    25501 gcaaattccg ttatttttat agcatctaaa agattcagct tttccactga tctttcacca
    25561 cttttcagct tttttattgt gcttaaattg ataccagtac cttttgatat tgaattggaa
    25621 gtaaaatcat taagggtttt ttttattaaa ttcatatccg caattccagc gttcataaga
    25681 atgaagattc tcctctcata gactactttg aaaatggtac tacagtggct gtttatatat
    25741 ttcagtaggt tactgtagtg gtcatcttat tataattaga aaaggaacct aaatcaaaaa
    25801 tgctaaagaa aattttcata gtattaagcc tctcatgact aaagccacgg aattcttgat
    25861 aaaagagtat acttgcaagc ctaaacagcg gtatctctta ttaaccaagc tatccccgtt
    25921 gttcctaagg ttcttgatgt tttctaaggc aatgcttgta ttcatagccc ttggtcaaaa
    25981 tattgatact atcgttgacg tttcgattat gatgtatacg gcaaatagga caagtccatt
    26041 ctcgaattgt gagtgacttc ttaccatctg tgtgtctaca ttctgaacca atttgactag
    26101 acggaaatca tttatctatc ttaatgattt tttgacagaa gtatcgaaaa tctttacata
    26161 atctgggtga tgtattctta tgcagatgag agcgttggct ccgtattcat aaccagtagc
    26221 ctctaaaacg tagtccatcg gtgggatacc tagcttgcgg ttgccagcgg ctttcgcagt
    26281 cttcttgtga tcaaatcaag catctcattg agcactagta ttttgaagat aatacttgag
    26341 tttttcggct tttagaagtt cacttcatct tccatcatcc atggaaatcc cactcaccca
    26401 tacgcaggcc tccaaaaatt cttcaacagt ggttttttca gacatttttc tgaatacctc
    26461 cttgtttttt gatacgccag atagtcagga aaaaggcaac aaaacacaaa acaaagctgc
    26521 ctaaaaaggt aatcttcatt ccatataaaa agacatccgg ccgatttgcg atatagttag
    26581 ttacttgctg gcccatctta gcagacatcg cttgatataa gaccgtcgta gaaagcgaaa
    26641 tcccgctgac catccccaaa tttcgggcaa aggaattcat cgagcctgcc agtcccaagt
    26701 tttcgatagt gaccgagctc ataaccatcg tattattggg ggactggaac aaggcattgc
    26761 cggcacccat gatggcagta gcgacaatgt atagccacaa aggagagtcc actgacaaaa
    26821 acatatacat gacctgggtt accgacaata agcccaagcc tactaatacc aaaatctccg
    26881 ggccaacccg gtcggtcagg tagccgctta aaggcgcccc gacgaccatc atcaaaggaa
    26941 agaccatcat caacagccca gcatagctag gactcaaccc gcgagcattt tgcagataaa
    27001 aaggaatcac tacattgaca aagaagttgg aagaaaaaat caacaaggct gtaatcaagc
    27061 tcatggtaaa aatcttgttc ttaaaaatac cgaaagaaat caatggagag gctgctttct
    27121 tttcaacttt taaaaagctg acaaaggcaa tcactgaaat tacgaacagt acaaggggga
    27181 ctggatggaa aaagccactt tcttgcccca aaaagattgc tccaaaaaag ctgataatcg
    27241 tagccgcaat ggcagcaaag ccccctccat cgattgtatg gccgctcatg gtgatatctt
    27301 ttggtaagaa tttttcagca atcaacatcg ttacgattcc caccggtaca ttgatccaaa
    27361 aaatgtacgc ccaagaaaat tgggacaaaa tcaagccgcc aagacctggt ccagcaatcg
    27421 agcctaatga aacaaacgca cctattgctc ccaatgcccg accacgttca tgtatgggaa
    27481 agacttccgt gatgattccg gcatttgtag ccatagtcat actagcacca atggcttgta
    27541 ccacccgtgc gaacaataaa atacctagcg aatgattgaa cccacacaac agcgatccca
    27601 agacaaagat cagcgtacca atccgaaata ctttgatttt cccacggcta tcgccaattt
    27661 taccaaacag caacaaacaa gaacagacaa tcatcagata aacggacact acccattccg
    27721 cttgattcat tggtatgccc atatcatctg agatggtagg tagtgcaata ttgacaatac
    27781 tgccatccaa tgtggacatg aaggtaaaca tagatatagc aatcaaaatc caccagcgat
    27841 ttttttggac ttttgcatct tcttgataac tatacataat aggatttcct ttctaatgat
    27901 cgtgtagcta agaacaatat aacctatgtc tactgcccaa caacttctcg cgactctagc
    27961 cataggaagc ttgagtaatt tcgatttgat atcctcatgt aaccatgtaa gttggagatg
    28021 ctttatttga acttactcta tagtaactag tgataattcg ctttactttc ttctagcaat
    28081 ttctaaaagt cgacttcatt gttcttcaga ttaatagtgt gagctatctc gtgagacgta
    28141 agcaaatttc cattctactt tttcccaatt ttttcatatt tatggtgttg cacctatttg
    28201 ttttccaatt tgcttttcaa atacattgct atgatattca attccatagg attatactaa
    28261 a
//
LOCUS       DAYYHB010000029        28098 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000029 DAYYHB010000000
VERSION     DAYYHB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.029"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..695
                     /locus_tag="ACHA6B_001231"
     CDS             24..695
                     /locus_tag="ACHA6B_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8948816.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="ACHA6B_001232"
     CDS             754..1194
                     /locus_tag="ACHA6B_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948817.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="ACHA6B_001233"
     CDS             1206..1874
                     /locus_tag="ACHA6B_001233"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8948818.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="ACHA6B_001234"
     CDS             1938..2561
                     /locus_tag="ACHA6B_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8948819.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="ACHA6B_001235"
     CDS             2617..3003
                     /locus_tag="ACHA6B_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8948820.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="ACHA6B_001236"
     CDS             3216..4607
                     /locus_tag="ACHA6B_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8948821.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5218..5586
                     /locus_tag="ACHA6B_001237"
     CDS             5218..5586
                     /locus_tag="ACHA6B_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8948822.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5766..6707
                     /locus_tag="ACHA6B_001238"
     CDS             5766..6707
                     /locus_tag="ACHA6B_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8948823.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6776..7507
                     /locus_tag="ACHA6B_001239"
     CDS             6776..7507
                     /locus_tag="ACHA6B_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8948824.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7651..9831
                     /locus_tag="ACHA6B_001240"
     CDS             7651..9831
                     /locus_tag="ACHA6B_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8948825.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9851..10333
                     /locus_tag="ACHA6B_001241"
     CDS             9851..10333
                     /locus_tag="ACHA6B_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8948826.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10349..11110
                     /locus_tag="ACHA6B_001242"
     CDS             10349..11110
                     /locus_tag="ACHA6B_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8948827.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11241..12575
                     /gene="murC"
                     /locus_tag="ACHA6B_001243"
     CDS             11241..12575
                     /gene="murC"
                     /locus_tag="ACHA6B_001243"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8948828.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12882..13367
                     /locus_tag="ACHA6B_001244"
     CDS             12882..13367
                     /locus_tag="ACHA6B_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8948829.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13360..14043
                     /locus_tag="ACHA6B_001245"
     CDS             13360..14043
                     /locus_tag="ACHA6B_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8948830.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14169..16814
                     /gene="polA"
                     /locus_tag="ACHA6B_001246"
     CDS             14169..16814
                     /gene="polA"
                     /locus_tag="ACHA6B_001246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8948831.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16859..17695
                     /gene="mutM"
                     /locus_tag="ACHA6B_001247"
     CDS             16859..17695
                     /gene="mutM"
                     /locus_tag="ACHA6B_001247"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8948832.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17659..18288
                     /gene="coaE"
                     /locus_tag="ACHA6B_001248"
     CDS             17659..18288
                     /gene="coaE"
                     /locus_tag="ACHA6B_001248"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8948833.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18340..18672
                     /locus_tag="ACHA6B_001249"
     CDS             18340..18672
                     /locus_tag="ACHA6B_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8948834.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18984..19475
                     /gene="nrdR"
                     /locus_tag="ACHA6B_001250"
     CDS             18984..19475
                     /gene="nrdR"
                     /locus_tag="ACHA6B_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8948835.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19498..20871
                     /locus_tag="ACHA6B_001251"
     CDS             19498..20871
                     /locus_tag="ACHA6B_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8948836.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20871..21797
                     /gene="dnaI"
                     /locus_tag="ACHA6B_001252"
     CDS             20871..21797
                     /gene="dnaI"
                     /locus_tag="ACHA6B_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8948837.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21926..22366
                     /locus_tag="ACHA6B_001253"
     CDS             21926..22366
                     /locus_tag="ACHA6B_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8948838.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22510..23211
                     /locus_tag="ACHA6B_001254"
     CDS             22510..23211
                     /locus_tag="ACHA6B_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8948839.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23208..24329
                     /locus_tag="ACHA6B_001255"
     CDS             23208..24329
                     /locus_tag="ACHA6B_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8948840.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24344..25576
                     /gene="pepT"
                     /locus_tag="ACHA6B_001256"
     CDS             24344..25576
                     /gene="pepT"
                     /locus_tag="ACHA6B_001256"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8948841.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFLNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25708..26616
                     /locus_tag="ACHA6B_001257"
     CDS             25708..26616
                     /locus_tag="ACHA6B_001257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8948842.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26651..26923
                     /locus_tag="ACHA6B_001258"
     CDS             26651..26923
                     /locus_tag="ACHA6B_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8948843.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26934..27980
                     /locus_tag="ACHA6B_001259"
     CDS             26934..27980
                     /locus_tag="ACHA6B_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8948844.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9444 a         4849 c         6117 g         7688 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg
     5221 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
     5281 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
     5341 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
     5401 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
     5461 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
     5521 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
     5581 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
     5641 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
     5701 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
     5761 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
     5821 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
     5881 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
     5941 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
     6001 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
     6061 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
     6121 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
     6181 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     6241 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     6301 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     6361 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     6421 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     6481 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     6541 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     6601 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     6661 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     6721 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     6781 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     6841 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     6901 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     6961 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     7021 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     7081 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     7141 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     7201 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     7261 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     7321 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     7381 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     7441 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     7501 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     7561 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     7621 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     7681 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     7741 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     7801 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     7861 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     7921 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     7981 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     8041 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     8101 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     8161 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     8221 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     8281 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     8341 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     8401 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     8461 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     8521 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     8581 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     8641 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     8701 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     8761 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     8821 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     8881 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     8941 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     9001 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     9061 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     9121 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     9181 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     9241 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     9301 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     9361 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     9421 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     9481 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     9541 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     9601 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     9661 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     9721 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     9781 acgaaaaaag gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     9841 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     9901 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     9961 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
    10021 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
    10081 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
    10141 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
    10201 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
    10261 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
    10321 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
    10381 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
    10441 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
    10501 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
    10561 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
    10621 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
    10681 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
    10741 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
    10801 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
    10861 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
    10921 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
    10981 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
    11041 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
    11101 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
    11161 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
    11221 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
    11281 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
    11341 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
    11401 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
    11461 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
    11521 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
    11581 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
    11641 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
    11701 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
    11761 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
    11821 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
    11881 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
    11941 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
    12001 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
    12061 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
    12121 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
    12181 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
    12241 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
    12301 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
    12361 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
    12421 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
    12481 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
    12541 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
    12601 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
    12661 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
    12721 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
    12781 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
    12841 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
    12901 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
    12961 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
    13021 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
    13081 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
    13141 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
    13201 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
    13261 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
    13321 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca
    13381 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
    13441 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
    13501 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
    13561 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
    13621 tctcttttga atggcataac attagcggtt actcttgcat actacgatat tggaacagta
    13681 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
    13741 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
    13801 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
    13861 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
    13921 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
    13981 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
    14041 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
    14101 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
    14161 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
    14221 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
    14281 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
    14341 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
    14401 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
    14461 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
    14521 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
    14581 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
    14641 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
    14701 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
    14761 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
    14821 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
    14881 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
    14941 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
    15001 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
    15061 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
    15121 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg
    15181 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    15241 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    15301 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    15361 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    15421 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    15481 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    15541 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    15601 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    15661 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    15721 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    15781 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    15841 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    15901 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    15961 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    16021 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    16081 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    16141 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    16201 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    16261 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    16321 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    16381 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    16441 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    16501 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    16561 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    16621 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    16681 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    16741 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    16801 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    16861 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    16921 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    16981 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    17041 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    17101 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    17161 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    17221 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    17281 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    17341 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    17401 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    17461 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    17521 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    17581 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    17641 acaaaggggg acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt
    17701 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    17761 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    17821 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    17881 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    17941 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    18001 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    18061 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    18121 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    18181 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaaaa
    18241 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    18301 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    18361 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    18421 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    18481 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    18541 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    18601 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    18661 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    18721 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    18781 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    18841 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    18901 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    18961 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    19021 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    19081 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    19141 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    19201 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    19261 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    19321 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    19381 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    19441 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    19501 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    19561 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    19621 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    19681 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    19741 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    19801 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    19861 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    19921 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    19981 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    20041 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    20101 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    20161 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    20221 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    20281 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    20341 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    20401 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    20461 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    20521 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    20581 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    20641 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    20701 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    20761 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    20821 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    20881 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    20941 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    21001 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    21061 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    21121 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    21181 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    21241 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    21301 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    21361 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    21421 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    21481 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    21541 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    21601 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    21661 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    21721 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    21781 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    21841 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    21901 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    21961 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    22021 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    22081 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    22141 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    22201 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    22261 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    22321 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    22381 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    22441 gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg
    22501 agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc
    22561 cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg
    22621 atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa
    22681 tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca
    22741 aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg
    22801 ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga
    22861 caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa
    22921 gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa
    22981 atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc
    23041 gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt
    23101 caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa
    23161 aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg
    23221 gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag
    23281 acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat
    23341 tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg
    23401 ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa
    23461 aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg
    23521 atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga
    23581 gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg
    23641 atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt
    23701 ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac
    23761 aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta
    23821 ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg
    23881 aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac
    23941 gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag
    24001 cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc
    24061 gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag
    24121 cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg
    24181 ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg
    24241 agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa
    24301 caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt
    24361 gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac
    24421 tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt
    24481 aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag
    24541 caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt
    24601 taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt
    24661 actagatgcg gaagaacgct atacattgaa tacaaaagat tttctaaatt tgaaaaatta
    24721 tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc
    24781 agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca
    24841 cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt
    24901 cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga
    24961 gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt
    25021 acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca
    25081 taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca
    25141 tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca
    25201 tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa
    25261 tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa
    25321 agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct
    25381 tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta
    25441 catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga
    25501 atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt
    25561 gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat
    25621 ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt
    25681 ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt
    25741 tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag
    25801 cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta
    25861 gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg
    25921 attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt
    25981 ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa
    26041 gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat
    26101 gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt
    26161 caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg
    26221 ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa
    26281 tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac
    26341 tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc
    26401 cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt
    26461 acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga
    26521 acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta
    26581 acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg
    26641 aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa
    26701 tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg
    26761 tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat
    26821 ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc
    26881 aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt
    26941 ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca
    27001 ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca
    27061 gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc
    27121 gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct
    27181 tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct
    27241 cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag
    27301 cactccatat gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca
    27361 tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta
    27421 tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc
    27481 aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg
    27541 tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag
    27601 acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg
    27661 tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt
    27721 cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt
    27781 cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt
    27841 cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt
    27901 atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga
    27961 ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa
    28021 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
    28081 attttgtttt tttaagat
//
LOCUS       DAYYHB010000030        27952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000030 DAYYHB010000000
VERSION     DAYYHB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.031"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(418..1362)
                     /locus_tag="ACHA6B_001260"
     CDS             complement(418..1362)
                     /locus_tag="ACHA6B_001260"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8948845.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1658..2602)
                     /locus_tag="ACHA6B_001261"
     CDS             complement(1658..2602)
                     /locus_tag="ACHA6B_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8948846.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2823..4037
                     /locus_tag="ACHA6B_001262"
     CDS             2823..4037
                     /locus_tag="ACHA6B_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8948847.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4059..6074
                     /locus_tag="ACHA6B_001263"
     CDS             4059..6074
                     /locus_tag="ACHA6B_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8948848.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6157..7530)
                     /locus_tag="ACHA6B_001264"
     CDS             complement(6157..7530)
                     /locus_tag="ACHA6B_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8948849.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7556..7840)
                     /locus_tag="ACHA6B_001265"
     CDS             complement(7556..7840)
                     /locus_tag="ACHA6B_001265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8948850.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7842..9857)
                     /locus_tag="ACHA6B_001266"
     CDS             complement(7842..9857)
                     /locus_tag="ACHA6B_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8948851.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10036..10341)
                     /locus_tag="ACHA6B_001267"
     CDS             complement(10036..10341)
                     /locus_tag="ACHA6B_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8948852.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10430..11461)
                     /locus_tag="ACHA6B_001268"
     CDS             complement(10430..11461)
                     /locus_tag="ACHA6B_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8948853.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11668..12543
                     /locus_tag="ACHA6B_001269"
     CDS             11668..12543
                     /locus_tag="ACHA6B_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8948854.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12695..13537)
                     /locus_tag="ACHA6B_001270"
     CDS             complement(12695..13537)
                     /locus_tag="ACHA6B_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8948855.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13629..15506)
                     /locus_tag="ACHA6B_001271"
     CDS             complement(13629..15506)
                     /locus_tag="ACHA6B_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8948856.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15503..16924)
                     /locus_tag="ACHA6B_001272"
     CDS             complement(15503..16924)
                     /locus_tag="ACHA6B_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8948857.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16927..20316)
                     /locus_tag="ACHA6B_001273"
     CDS             complement(16927..20316)
                     /locus_tag="ACHA6B_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8948858.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20573..21964
                     /locus_tag="ACHA6B_001274"
     CDS             20573..21964
                     /locus_tag="ACHA6B_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948859.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22037..22684)
                     /locus_tag="ACHA6B_001275"
     CDS             complement(22037..22684)
                     /locus_tag="ACHA6B_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948860.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22692..23378)
                     /locus_tag="ACHA6B_001276"
     CDS             complement(22692..23378)
                     /locus_tag="ACHA6B_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948861.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23384..24457)
                     /locus_tag="ACHA6B_001277"
     CDS             complement(23384..24457)
                     /locus_tag="ACHA6B_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948862.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24679..25353
                     /locus_tag="ACHA6B_001278"
     CDS             24679..25353
                     /locus_tag="ACHA6B_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8948863.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25358..26698
                     /locus_tag="ACHA6B_001279"
     CDS             25358..26698
                     /locus_tag="ACHA6B_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8948864.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26847..27833)
                     /locus_tag="ACHA6B_001280"
     CDS             complement(26847..27833)
                     /locus_tag="ACHA6B_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8948865.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8157 a         5272 c         5062 g         9461 t
ORIGIN      
        1 gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc aaaatctgct
       61 tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat
      121 gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt
      181 gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg
      241 cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc
      301 gattaatttt aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt
      361 aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta
      421 taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc
      481 ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg
      541 cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa
      601 tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc
      661 atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc
      721 aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg
      781 tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa
      841 gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa
      901 acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc
      961 gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc
     1021 gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac
     1081 taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc
     1141 agcatcttta cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc
     1201 cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa
     1261 tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa
     1321 acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt
     1381 cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt
     1441 taattataat agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca
     1501 gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg
     1561 aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac
     1621 aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat
     1681 tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc
     1741 cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa
     1801 agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt
     1861 tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag
     1921 aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat
     1981 ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg
     2041 tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat
     2101 tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat
     2161 gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg
     2221 ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa
     2281 tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt
     2341 cactttcttc catctctatc ctttctccat taataatttg taaacatgta ccgctataca
     2401 tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt
     2461 tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct
     2521 gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt
     2581 cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct
     2641 cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa
     2701 gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc
     2761 tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga
     2821 acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga
     2881 cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt
     2941 ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt
     3001 tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag
     3061 tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag
     3121 cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga
     3181 ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc
     3241 aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc
     3301 aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg
     3361 ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa
     3421 tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat
     3481 ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa
     3541 ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt
     3601 ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg
     3661 gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga
     3721 tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc
     3781 aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag
     3841 gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa
     3901 cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc
     3961 tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac
     4021 aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag
     4081 atagatggct ttggtatcct ggagactttg agatccgaca tggactgcta caaaatttcc
     4141 aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga
     4201 acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc
     4261 aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt
     4321 gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga
     4381 tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg
     4441 aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa
     4501 tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct
     4561 tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata
     4621 tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt
     4681 gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct
     4741 atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt
     4801 tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt
     4861 cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac
     4921 gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt
     4981 tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca
     5041 agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc
     5101 actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat
     5161 taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag
     5221 attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag
     5281 tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat
     5341 atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac
     5401 aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat
     5461 ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta
     5521 acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga
     5581 ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga
     5641 atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa
     5701 ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag
     5761 atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac
     5821 gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta
     5881 atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc
     5941 ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa
     6001 tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa
     6061 aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc
     6121 tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat
     6181 gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat
     6241 gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg
     6301 tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg
     6361 aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc
     6421 ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg
     6481 tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag
     6541 tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa
     6601 aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg
     6661 gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc
     6721 agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag
     6781 ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat
     6841 caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat
     6901 gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata
     6961 accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa
     7021 cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa
     7081 gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga
     7141 aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag
     7201 taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg
     7261 caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt
     7321 ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag
     7381 tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt
     7441 ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat
     7501 tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt
     7561 cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca
     7621 aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga
     7681 agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact
     7741 cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac
     7801 ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt
     7861 atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta
     7921 ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca
     7981 gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt
     8041 tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt
     8101 aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata
     8161 gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg
     8221 attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat
     8281 tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt
     8341 aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc
     8401 gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt
     8461 acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga
     8521 gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt
     8581 tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat
     8641 aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc
     8701 acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat
     8761 agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac
     8821 ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg
     8881 atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga
     8941 taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc
     9001 atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca
     9061 tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt
     9121 tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc
     9181 aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa
     9241 ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa
     9301 atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt
     9361 aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt
     9421 gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac
     9481 gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact
     9541 gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc
     9601 gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat
     9661 cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata
     9721 cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt
     9781 acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa
     9841 ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt
     9901 ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac
     9961 gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga
    10021 attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt
    10081 ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg
    10141 ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc
    10201 accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc
    10261 tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt
    10321 ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac
    10381 aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc
    10441 cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt
    10501 cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga
    10561 cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat
    10621 ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag
    10681 gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc
    10741 ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta
    10801 tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt
    10861 tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa
    10921 cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat
    10981 gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat
    11041 cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc
    11101 tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg
    11161 cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg
    11221 ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct
    11281 caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc
    11341 acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg
    11401 aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca
    11461 tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt
    11521 tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga
    11581 aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc
    11641 agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag
    11701 ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca
    11761 gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt
    11821 cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg
    11881 gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca
    11941 agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata
    12001 aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac
    12061 aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc
    12121 cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct
    12181 tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt
    12241 cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg
    12301 ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata
    12361 tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat
    12421 tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc
    12481 aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact
    12541 taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat
    12601 catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg
    12661 attcgaatag atatattata gaaatgagga atagctaact tctgtttctt attttgaacg
    12721 attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat
    12781 acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat
    12841 gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac
    12901 aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg
    12961 gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat
    13021 agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc
    13081 ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt
    13141 ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat
    13201 ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat
    13261 cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt
    13321 ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat
    13381 tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca
    13441 gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca
    13501 tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg
    13561 tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg
    13621 caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga
    13681 aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta
    13741 cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat
    13801 tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc
    13861 gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt
    13921 gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat
    13981 gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct
    14041 ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca
    14101 gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct
    14161 tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat
    14221 ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat
    14281 ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat
    14341 aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta
    14401 tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat
    14461 gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc
    14521 aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt
    14581 gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca
    14641 ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc
    14701 aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga
    14761 ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg
    14821 acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct
    14881 gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca
    14941 ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta
    15001 taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg
    15061 gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt
    15121 tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact
    15181 ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta
    15241 tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat
    15301 ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt
    15361 aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact
    15421 ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata
    15481 acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc
    15541 agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt
    15601 ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt
    15661 ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta
    15721 aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg
    15781 aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg
    15841 tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga
    15901 atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac
    15961 tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt
    16021 tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg
    16081 tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt
    16141 gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta
    16201 aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat
    16261 agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg
    16321 taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt
    16381 cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag
    16441 gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa
    16501 tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga
    16561 taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg
    16621 caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat
    16681 ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg
    16741 tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg
    16801 ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct
    16861 gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt
    16921 tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca
    16981 accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg
    17041 tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag
    17101 aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt
    17161 aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt
    17221 tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc
    17281 taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc
    17341 ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca
    17401 tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat
    17461 ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac
    17521 tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg
    17581 gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc
    17641 tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac
    17701 ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt
    17761 tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct
    17821 taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa
    17881 tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat
    17941 agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt
    18001 tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc
    18061 tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc
    18121 ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt
    18181 tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt
    18241 aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat
    18301 ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt
    18361 tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc
    18421 attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg
    18481 atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc
    18541 ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt
    18601 gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa
    18661 tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc
    18721 ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc
    18781 gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag
    18841 accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc
    18901 tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag
    18961 attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc
    19021 ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg
    19081 tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc
    19141 atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc
    19201 atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat
    19261 ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt
    19321 gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt
    19381 tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag
    19441 atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa
    19501 tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt
    19561 tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat
    19621 accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc
    19681 tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat attctgtagg
    19741 aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg
    19801 gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac
    19861 tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc
    19921 gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc
    19981 gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata
    20041 agcatagcca tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat
    20101 ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt
    20161 ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc
    20221 tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat
    20281 gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt
    20341 ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta
    20401 ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc
    20461 aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag
    20521 ggttgaggga gaaaaacata tgatcaggtt gttggaagga aactatagaa agatggaaaa
    20581 aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg
    20641 aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg
    20701 aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt
    20761 accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt
    20821 aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat
    20881 cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt
    20941 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
    21001 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
    21061 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
    21121 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
    21181 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
    21241 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
    21301 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
    21361 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
    21421 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
    21481 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
    21541 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
    21601 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
    21661 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
    21721 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
    21781 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
    21841 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
    21901 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
    21961 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
    22021 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
    22081 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
    22141 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
    22201 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
    22261 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
    22321 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
    22381 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
    22441 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
    22501 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
    22561 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
    22621 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
    22681 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
    22741 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
    22801 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
    22861 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
    22921 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
    22981 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
    23041 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
    23101 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
    23161 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
    23221 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
    23281 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
    23341 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
    23401 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
    23461 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
    23521 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
    23581 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
    23641 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
    23701 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
    23761 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
    23821 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
    23881 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
    23941 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
    24001 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
    24061 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
    24121 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
    24181 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
    24241 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
    24301 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
    24361 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
    24421 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
    24481 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
    24541 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
    24601 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
    24661 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
    24721 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
    24781 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
    24841 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
    24901 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
    24961 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
    25021 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
    25081 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
    25141 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
    25201 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
    25261 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
    25321 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
    25381 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
    25441 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
    25501 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
    25561 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
    25621 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
    25681 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
    25741 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
    25801 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
    25861 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
    25921 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
    25981 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
    26041 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
    26101 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
    26161 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
    26221 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
    26281 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
    26341 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
    26401 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
    26461 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
    26521 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
    26581 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
    26641 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
    26701 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
    26761 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
    26821 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
    26881 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
    26941 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
    27001 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
    27061 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
    27121 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
    27181 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
    27241 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
    27301 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
    27361 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
    27421 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
    27481 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
    27541 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
    27601 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
    27661 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
    27721 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
    27781 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
    27841 cctcctagca aatagattta tgattggtat attgttactt ataaatttat tctataggag
    27901 acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta at
//
LOCUS       DAYYHB010000031        27630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000031 DAYYHB010000000
VERSION     DAYYHB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.032"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..309)
                     /locus_tag="ACHA6B_001281"
     CDS             complement(7..309)
                     /locus_tag="ACHA6B_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8948866.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(567..680)
                     /locus_tag="ACHA6B_001282"
                     /pseudo
     CDS             complement(567..680)
                     /locus_tag="ACHA6B_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1063..2295)
                     /locus_tag="ACHA6B_001283"
     CDS             complement(1063..2295)
                     /locus_tag="ACHA6B_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8948867.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2549..3118
                     /locus_tag="ACHA6B_001284"
     CDS             2549..3118
                     /locus_tag="ACHA6B_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948868.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3296..3736)
                     /locus_tag="ACHA6B_001285"
     CDS             complement(3296..3736)
                     /locus_tag="ACHA6B_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8948869.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3894..4658
                     /gene="map"
                     /locus_tag="ACHA6B_001286"
     CDS             3894..4658
                     /gene="map"
                     /locus_tag="ACHA6B_001286"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8948870.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4690..5613
                     /locus_tag="ACHA6B_001287"
     CDS             4690..5613
                     /locus_tag="ACHA6B_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8948871.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5689..6828
                     /locus_tag="ACHA6B_001288"
     CDS             5689..6828
                     /locus_tag="ACHA6B_001288"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8948872.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6821..7621
                     /locus_tag="ACHA6B_001289"
     CDS             6821..7621
                     /locus_tag="ACHA6B_001289"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8948873.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7621..8448
                     /locus_tag="ACHA6B_001290"
     CDS             7621..8448
                     /locus_tag="ACHA6B_001290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8948874.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8453..9160
                     /locus_tag="ACHA6B_001291"
     CDS             8453..9160
                     /locus_tag="ACHA6B_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8948875.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            9260..10126
                     /gene="rfbA"
                     /locus_tag="ACHA6B_001292"
     CDS             9260..10126
                     /gene="rfbA"
                     /locus_tag="ACHA6B_001292"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8948876.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10140..10712
                     /gene="rfbC"
                     /locus_tag="ACHA6B_001293"
     CDS             10140..10712
                     /gene="rfbC"
                     /locus_tag="ACHA6B_001293"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8948877.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10734..11762
                     /gene="rfbB"
                     /locus_tag="ACHA6B_001294"
     CDS             10734..11762
                     /gene="rfbB"
                     /locus_tag="ACHA6B_001294"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8948878.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11860..12711
                     /gene="rfbD"
                     /locus_tag="ACHA6B_001295"
     CDS             11860..12711
                     /gene="rfbD"
                     /locus_tag="ACHA6B_001295"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8948879.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12746..14779
                     /locus_tag="ACHA6B_001296"
     CDS             12746..14779
                     /locus_tag="ACHA6B_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948880.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14823..16103
                     /locus_tag="ACHA6B_001297"
     CDS             14823..16103
                     /locus_tag="ACHA6B_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8948881.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16313..17119
                     /locus_tag="ACHA6B_001298"
     CDS             16313..17119
                     /locus_tag="ACHA6B_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948882.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17131..18351
                     /locus_tag="ACHA6B_001299"
     CDS             17131..18351
                     /locus_tag="ACHA6B_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948883.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18341..19927
                     /locus_tag="ACHA6B_001300"
     CDS             18341..19927
                     /locus_tag="ACHA6B_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8948884.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19966..22104
                     /locus_tag="ACHA6B_001301"
     CDS             19966..22104
                     /locus_tag="ACHA6B_001301"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8948885.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22472..23455)
                     /locus_tag="ACHA6B_001302"
     CDS             complement(22472..23455)
                     /locus_tag="ACHA6B_001302"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8948886.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23833..25224
                     /locus_tag="ACHA6B_001303"
     CDS             23833..25224
                     /locus_tag="ACHA6B_001303"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8948887.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25240..26280
                     /locus_tag="ACHA6B_001304"
     CDS             25240..26280
                     /locus_tag="ACHA6B_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8948888.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRKIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26299..27384
                     /locus_tag="ACHA6B_001305"
     CDS             26299..27384
                     /locus_tag="ACHA6B_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8948889.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27417..>27630
                     /locus_tag="ACHA6B_001306"
     CDS             27417..>27630
                     /locus_tag="ACHA6B_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFM8948890.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIV"
BASE COUNT         8877 a         4594 c         5563 g         8596 t
ORIGIN      
        1 ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat agtcataacg
       61 ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa agtatacacc
      121 cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag aatagtagag
      181 attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc atactttata
      241 aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg tttttccttc
      301 catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt ttgtgaaggc
      361 ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg tgccaaaaaa
      421 attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat ttcctgcaat
      481 aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa cccctgtatc
      541 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
      601 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
      661 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
      721 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
      781 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
      841 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      901 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      961 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
     1021 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
     1081 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
     1141 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
     1201 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
     1261 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
     1321 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
     1381 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
     1441 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
     1501 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
     1561 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
     1621 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
     1681 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
     1741 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
     1801 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
     1861 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
     1921 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
     1981 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
     2041 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
     2101 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
     2161 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
     2221 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
     2281 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
     2341 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
     2401 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
     2461 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
     2521 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
     2581 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
     2641 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
     2701 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
     2761 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
     2821 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
     2881 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
     2941 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
     3001 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
     3061 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
     3121 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
     3181 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
     3241 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
     3301 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
     3361 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     3421 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     3481 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     3541 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     3601 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     3661 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     3721 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     3781 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
     3841 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     3901 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     3961 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     4021 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     4081 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     4141 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     4201 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     4261 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     4321 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     4381 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     4441 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     4501 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     4561 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     4621 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     4681 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     4741 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     4801 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     4861 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     4921 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     4981 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     5041 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     5101 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     5161 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     5221 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     5281 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     5341 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     5401 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     5461 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     5521 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     5581 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     5641 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     5701 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     5761 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     5821 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     5881 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     5941 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     6001 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     6061 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     6121 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     6181 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     6241 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     6301 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     6361 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     6421 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     6481 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     6541 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     6601 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     6661 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     6721 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     6781 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     6841 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     6901 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     6961 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     7021 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     7081 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     7141 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     7201 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     7261 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     7321 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     7381 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     7441 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     7501 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     7561 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     7621 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     7681 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     7741 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     7801 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     7861 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     7921 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     7981 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     8041 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     8101 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     8161 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     8221 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     8281 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     8341 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     8401 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     8461 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     8521 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     8581 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     8641 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     8701 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     8761 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     8821 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     8881 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     8941 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     9001 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     9061 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     9121 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     9181 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     9241 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     9301 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     9361 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     9421 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     9481 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     9541 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     9601 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     9661 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     9721 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     9781 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     9841 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     9901 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     9961 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
    10021 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
    10081 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
    10141 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
    10201 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
    10261 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
    10321 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
    10381 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
    10441 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
    10501 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
    10561 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
    10621 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    10681 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    10741 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    10801 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    10861 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    10921 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    10981 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    11041 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    11101 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    11161 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    11221 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    11281 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    11341 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    11401 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    11461 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    11521 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
    11581 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
    11641 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    11701 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    11761 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    11821 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
    11881 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
    11941 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
    12001 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
    12061 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
    12121 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
    12181 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    12241 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    12301 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    12361 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    12421 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    12481 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
    12541 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    12601 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
    12661 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    12721 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
    12781 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
    12841 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
    12901 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
    12961 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
    13021 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
    13081 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
    13141 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
    13201 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
    13261 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
    13321 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
    13381 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
    13441 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
    13501 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
    13561 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
    13621 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
    13681 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
    13741 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
    13801 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
    13861 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
    13921 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
    13981 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
    14041 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
    14101 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
    14161 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
    14221 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
    14281 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
    14341 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
    14401 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
    14461 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
    14521 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
    14581 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
    14641 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
    14701 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
    14761 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
    14821 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
    14881 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
    14941 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
    15001 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
    15061 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
    15121 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
    15181 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
    15241 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    15301 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    15361 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
    15421 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    15481 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    15541 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    15601 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    15661 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
    15721 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    15781 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    15841 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    15901 tggaggatac attgacttca taccgatggg ttccgattat ttatatcctg ttggctatgg
    15961 ccgttcatcg atatgatgaa ccagaagtta cggaggaatt ctatgttatg gaagaggcat
    16021 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    16081 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    16141 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    16201 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    16261 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    16321 agatttgtat ttatttatga aggacattta tcagaacaga aaattactgc tacagttttc
    16381 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    16441 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    16501 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    16561 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    16621 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    16681 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    16741 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    16801 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    16861 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    16921 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16981 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    17041 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    17101 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    17161 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    17221 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    17281 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    17341 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    17401 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    17461 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    17521 atcattgaat ttgctgacat cggcgaccat atttatcaac ctgtaaaaac ctattcaagt
    17581 gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga tattttgatt
    17641 gtggatgaag cattggcagt aggagatctt gaatttcaac taaaatgtat ggaaaaattt
    17701 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat tagttcgatt
    17761 cgccgttttt gtgatcgttc ctattggttg aaaaatggaa aagttgtcga atatggtgat
    17821 acgatggaag taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt taaaacagta
    17881 gatcgaaata aaaaccaagt agaacgcttc gcagctccag atatcgtaga cgtgacgaaa
    17941 gccgaattgt tggataaaga taaaaatcca gtggatatgc tggaacaagg agaaaaacta
    18001 tacgttaaag taacctatga agtaaaagac gatacaatca aaaatccagt tttaggaatt
    18061 gctctgcgta cagtcgacaa tttttatgtg tgcggactca atactctact agatggtatc
    18121 aagatccctt ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa gttaggtttg
    18181 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    18241 gtatacaaga caaaatacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    18301 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    18361 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    18421 agttttagag tttggtcctg gaaacggccg attaacgaaa catttgatcg gagcaaaaca
    18481 atgtgaagtg agcattgtcg agctagataa ggaattgttc gattttgtca gcgagtttgc
    18541 ccaagatgga ttttatggag atatcgaaag ttttgaatgg gcaaactatt atgctggaca
    18601 aacatttgat tacgttttgt ttgccgatgt tttagaacat ttggtagatc ctggaaaaac
    18661 gttgaagaaa gttagagaat ttttgaatga agaaggagaa attctaatta cattccctaa
    18721 ccttgcgcat aattctgtca tgattgattt gttcaataat caattgccat gggcttctta
    18781 tggtttgcta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    18841 tgaaaaagca ggattataca tcaatattga agattatttg tacttagctg taggtgatac
    18901 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgttatgatt tcaaaatgcg
    18961 accatttggc gaagtctacc aatacttctt ctcattgatg aaacatccag tagcacaaag
    19021 ttccattgcg cagccgcaaa attctaatta tgtgaaagta ttagaagtaa tccagcaaac
    19081 gaaacaagat gaaataattc aacaaatacc attcaataat ttcacagggg aaaatgagac
    19141 actaagtttc catgtatcag aattagcaga acgaatggtg tttcgcttcg cgcagcaacc
    19201 tagctttatc gaattcagcg cagaagctgc tggagaaaaa ctcacattta tcgattcaaa
    19261 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat ggtaaagatt taccagagtt
    19321 ctttttaacg gatattgcag ggaaagaagt gactatccat tgtcactatc gttttatcgg
    19381 agaattgaca ccaacaatga aagaattgct ggaaacaatc cgtccaatgg ctgaagttac
    19441 aaagcagctt tctgagaaaa atgatgaatt agaaagagaa aatcattatc ttattgagaa
    19501 taatacaaga ttagatcaga cgttgaaaac aaccataaat cgctattgca cgttgttgga
    19561 aagtgatgaa tggacaatca aacatagatt agggcgacgt aagaaagaaa catcgaaaaa
    19621 aatccaagaa aaagagcttt ctctatgtat tgacgaaaaa atctgggatg cagaaacaaa
    19681 gatattgaaa atcatcgggt ggggaatcgc taattcggat cgccaaccat tgtcctataa
    19741 gttatcagcc gaccaatcac cattttttga agcaattcct gttttacgag aagaagtcaa
    19801 tcaagcaaaa cagctagctg aaggagcaaa agcaggattt gaactgcgga tcctttgcga
    19861 gaaggaacgt tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat ggatcattga
    19921 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19981 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    20041 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    20101 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    20161 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagcaatc
    20221 ttagacttcc aaacagcaag acatcaaaat ggcatagctg tgaaattaaa cggtaaatat
    20281 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    20341 ataaattatg caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa actggaattg
    20401 aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc tgatctgaaa
    20461 gagcaaagga aagaagtaca aggatttgcc taccgcccat tgatttccat acttatgcct
    20521 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact tgatcaaacg
    20581 tatgatcact gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    20641 tgtctagaat cctatcaagc aaaagatgat agaattaaag tcgttttccg acaagaaaat
    20701 ggacacatca gcctggcaac taattctgca ttggaaatgg cagaaggaga gtttatagcg
    20761 ctcttagata atgatgacga attacctcca tttgccttat atgaagtggc aaaagtgcta
    20821 aacgtacatc cagaactgga tttgatctac agtgacgaag ataaaatcga tgcagatggg
    20881 aatcgctttg acccgcactt caaagcagac tggtctcccg atacattgat gggaaataat
    20941 tatatctctc acttaggcgt atatcggaca agcattgtta aggaacttgg cggtttccgt
    21001 aaaggatatg aaggctctca agattatgat ttggttcttc gtgtgacaga acaaattcct
    21061 gcagaacaca tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat cccgggatca
    21121 actgcaagca atggagaagc taaaagctac atctatgatt ctggagtgaa agcattgaca
    21181 gatgctctat ctagaagaaa tatcaaggga agcgtacatc ctggcagaat ttctggtttt
    21241 tatgaaatca cttatgatgt tttgcaagaa gacttggtca gtgtgatcat tccaactaag
    21301 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttatcca
    21361 aattatgaaa tcattattgc agataatgga agtacagatc ctaaaatgca agaattattt
    21421 gcagaataca agcatcagct gaaagaccga tttatcgtcg aattaattga cattccattc
    21481 aattattcac gtatcaacaa tctggcagca gaaaaagcaa acggcaaata ctttttattc
    21541 ttgaataacg atacagaagt gattgaacct gactggatga cagcgatggt ttcttatgca
    21601 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    21661 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    21721 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    21781 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    21841 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    21901 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21961 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    22021 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    22081 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    22141 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    22201 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    22261 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    22321 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    22381 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    22441 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    22501 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    22561 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    22621 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    22681 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    22741 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    22801 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    22861 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    22921 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22981 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    23041 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    23101 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    23161 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    23221 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    23281 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    23341 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    23401 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    23461 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    23521 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    23581 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    23641 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    23701 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    23761 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
    23821 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    23881 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    23941 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    24001 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    24061 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    24121 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    24181 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    24241 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    24301 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    24361 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    24421 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    24481 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    24541 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    24601 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    24661 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    24721 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    24781 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    24841 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    24901 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    24961 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    25021 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    25081 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    25141 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    25201 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    25261 ataatcgtac cagtatacaa tgcagaagca actattggtc gaaagattga tattctaaaa
    25321 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    25381 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    25441 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    25501 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    25561 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    25621 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    25681 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    25741 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    25801 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
    25861 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
    25921 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
    25981 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
    26041 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
    26101 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
    26161 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
    26221 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
    26281 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
    26341 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
    26401 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
    26461 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
    26521 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
    26581 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
    26641 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
    26701 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
    26761 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
    26821 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
    26881 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
    26941 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
    27001 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
    27061 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
    27121 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
    27181 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
    27241 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
    27301 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
    27361 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
    27421 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
    27481 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
    27541 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
    27601 aaatttattg gggtggtttt tccattgtac
//
LOCUS       DAYYHB010000032        26929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000032 DAYYHB010000000
VERSION     DAYYHB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.033"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHA6B_001307"
     CDS             complement(<1..448)
                     /locus_tag="ACHA6B_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948891.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="ACHA6B_001308"
     CDS             complement(755..1852)
                     /locus_tag="ACHA6B_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8948892.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="ACHA6B_001309"
     CDS             complement(1960..3987)
                     /locus_tag="ACHA6B_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8948893.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4860)
                     /locus_tag="ACHA6B_001310"
     CDS             complement(4132..4860)
                     /locus_tag="ACHA6B_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8948894.1"
                     /translation="MKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            complement(5379..6278)
                     /locus_tag="ACHA6B_001311"
     CDS             complement(5379..6278)
                     /locus_tag="ACHA6B_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8948895.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(6288..6644)
                     /locus_tag="ACHA6B_001312"
     CDS             complement(6288..6644)
                     /locus_tag="ACHA6B_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8948896.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            6999..7295
                     /locus_tag="ACHA6B_001313"
     CDS             6999..7295
                     /locus_tag="ACHA6B_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8948897.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(7939..8892)
                     /locus_tag="ACHA6B_001314"
     CDS             complement(7939..8892)
                     /locus_tag="ACHA6B_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8948898.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(8915..9670)
                     /locus_tag="ACHA6B_001315"
     CDS             complement(8915..9670)
                     /locus_tag="ACHA6B_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948899.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(9667..10626)
                     /locus_tag="ACHA6B_001316"
     CDS             complement(9667..10626)
                     /locus_tag="ACHA6B_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8948900.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(10619..11569)
                     /locus_tag="ACHA6B_001317"
     CDS             complement(10619..11569)
                     /locus_tag="ACHA6B_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8948901.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            11719..12171
                     /locus_tag="ACHA6B_001318"
     CDS             11719..12171
                     /locus_tag="ACHA6B_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8948902.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(12260..14527)
                     /gene="gshAB"
                     /locus_tag="ACHA6B_001319"
     CDS             complement(12260..14527)
                     /gene="gshAB"
                     /locus_tag="ACHA6B_001319"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8948903.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14714..15712)
                     /gene="ftsY"
                     /locus_tag="ACHA6B_001320"
     CDS             complement(14714..15712)
                     /gene="ftsY"
                     /locus_tag="ACHA6B_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8948904.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(15723..16532)
                     /locus_tag="ACHA6B_001321"
     CDS             complement(15723..16532)
                     /locus_tag="ACHA6B_001321"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8948905.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(16541..20122)
                     /gene="smc"
                     /locus_tag="ACHA6B_001322"
     CDS             complement(16541..20122)
                     /gene="smc"
                     /locus_tag="ACHA6B_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8948906.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(20142..20828)
                     /gene="rnc"
                     /locus_tag="ACHA6B_001323"
     CDS             complement(20142..20828)
                     /gene="rnc"
                     /locus_tag="ACHA6B_001323"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8948907.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(21176..22957)
                     /locus_tag="ACHA6B_001324"
     CDS             complement(21176..22957)
                     /locus_tag="ACHA6B_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8948908.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(22983..23897)
                     /locus_tag="ACHA6B_001325"
     CDS             complement(22983..23897)
                     /locus_tag="ACHA6B_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948909.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(23924..24886)
                     /gene="opp4B"
                     /locus_tag="ACHA6B_001326"
     CDS             complement(23924..24886)
                     /gene="opp4B"
                     /locus_tag="ACHA6B_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8948910.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(24886..25830)
                     /locus_tag="ACHA6B_001327"
     CDS             complement(24886..25830)
                     /locus_tag="ACHA6B_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948911.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(25831..26835)
                     /locus_tag="ACHA6B_001328"
     CDS             complement(25831..26835)
                     /locus_tag="ACHA6B_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948912.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         7552 a         5687 c         4662 g         9028 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatc
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagcgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacattat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga cttgactgcc tagacttcaa tcaatattaa ttataattaa
     5161 ataaaaaatg tatattatat tttttttgtg attatcgttc aaaaaaagaa tacacgcttg
     5221 catctagtac tctgcgagtt acaaatcatc cgatcaaata aacattcgct gattattttc
     5281 cgcaaaaaaa gcagcatatc cacgataaat ggatctactg ctcttactta tccttatgtt
     5341 ttacaaggta ttttctttta cacattacta atgctgtctc aatacggaac gattgtcatc
     5401 gcttgttcaa attcctcttg caaatgttgt gtttgatagg cctgaaaagc tgggtgatgg
     5461 gcacgcaaga ggacacgtaa tttcatagga tcatagctgt ctagcaactg ttctttcaag
     5521 acaaaatctc gggtcagctg ccattcataa taataaccaa aaggtctgca gctgtaaatc
     5581 gtgtgaccga tggatttatc ttcaaagcga cgatgcgtat ggccaaaaac tacttgtttg
     5641 atatttgaat aacggtcaag taattctccg aatgattctg atcctaaaaa tgcatttaat
     5701 ttgttccaac gctcataagg agcagattga tacatgataa atgcccgttt aggaacgaaa
     5761 tgagtggata aaatcacctc aagttttttt gcttccagct ggtctagcaa attttttgca
     5821 gctgcaagca tttctgcatt gacctcgaga tctgttttcc cacgctggat gaaccgatca
     5881 taccaataca aacgcttcat tcgtacgatt gcctcgtaat ctttcaattc ggaaaactga
     5941 tagtcgtacc agccattcat ccctaaaaga acggtctgct catttaaagc aatataccgt
     6001 tcatttaaaa aatgtgggtc tggaaaatgg tccatcatct gttcattggg cacatctgcc
     6061 aactcgtgat tgccgaaatg aaatgtcgta gataatcctt tattgacgaa aaattcgaca
     6121 acagctaggc aacggtctac tttattagcc gtatctccgg ctagatgaag ccgagtgatc
     6181 ttgtgctttt gcaaaacttg ccaaagctgc atcagctctg attcgccgaa tttattgatg
     6241 tccacatgta ggtcgctgat gatcgccaat tttcccattt tatccctcta gtcttttcga
     6301 acaacttgca agttgccttt tctcaagccg cttggtcctt ttaattgtaa actattataa
     6361 gttggttcta aagcaagtgt ggatggatca tccatgtaaa gtattgcact gtctttgatt
     6421 ttgattggtc caactggtgt atcagccact acagaataaa gacttagatc tgtttgtgta
     6481 tcttttttga caatgatcaa tcgaaatgcc gtatccaatt ggcaactgac ttggctttct
     6541 gcaaatggac cgtcaccgtt ttcgatatct aataaaagtt gatcgtctgc ttctaattta
     6601 ttcttcaata cttcttgtgc ttcttttgtc acttctaatt tcatctgtat cacctcatgt
     6661 catagaataa tcactttatt catcagatgc aactaatttg tctggttctg actgttctca
     6721 atttattgga aagtaagtca ttcgtttatt atgatttctc ttatcacaaa agagacaact
     6781 ttcccttttc ttcgttcagt ttcttgaacg gaaaaagacc caaaaagcaa cctgttattt
     6841 gtttaacctt attcctttct taaataacaa aaaatactat aaaaaataaa aaacctttaa
     6901 caactattgg aaaaagaatt actttttgtt cacggattat taacaactct tcggtagagt
     6961 aaacttatca aataaaagac ttctttggag gaatcactat gaaggaatat ataaaaaaca
     7021 tctattttat cgaagaaaca caaaatatcg agggttctta tattgaagta aaaacactct
     7081 ttgtcaatga agataaaacc aaagcattgg atatttataa aaaattagct tcaaaaaaaa
     7141 cgaatagttt tggattgata ttaagcgagt ataaaatcaa agcagaagag agttatttct
     7201 atcaattatt aaaacggtgg agtaagcttc ccgccgattt ttatcgaaaa atgcagatca
     7261 tcaactatca accgcttgct gagacacatg cctaaataga ggttgtgaaa tcagtgcttt
     7321 gagctaatac cgaaggtcag ctgattgaac attgtttaga agagattgtg acaactgcga
     7381 tttgctttga gtaataagaa cgctggacag aaaatagctt ctcatatttt ctagtctagt
     7441 gggcttattg ccgagaagca gcagatggaa catcgtttag aagagattgt gacaactgcg
     7501 atttgctttg agtaataaga acgctggaca gaaaatagct tctcatattt tctagtctag
     7561 tggacttatt gccgagaagc agcagatgga acatcgttta aagaagagat cggtacaaaa
     7621 taaaaatatc taaatttaag ttttatctaa gtttcgcttt gtacctttga ttcaaatcaa
     7681 aggaggcgat cttgatgctg aaaaaatgga tcacactatt ttttgccact ccccattcta
     7741 gaatcaaccc gtatgatgta gataatgaat tcacgttgaa aaaagatttc tcaaataaat
     7801 aaaaaccatc cgtaaaatca tcaatacgat tttacggatg gtttttgata acttctttga
     7861 ggataggctc ttgctattta agaatgtaag aaacattatt gttacatagt atgagagttt
     7921 aatcctctgt tatttatgct atttaaatgc ttgattcaca tcttcgatca tcagtttcat
     7981 tgattctaaa ccgcccccgc ttaaatacca gacatctggt tcaagggaga tgatctgctg
     8041 atttttacca gcattcgttt gtgctaccag ttcattggca gaaatatcat ctttgctgtc
     8101 gtctccacca atagcttttg ttctgtctac tacaaataag atatccggat ttttttccag
     8161 cacatattca taagaaacac tttgaccgtg agtggatgct tcgatttgat catctgcttg
     8221 ttcaaagcca aataaatcat ggatgaaacc gaatctagaa ccagatccgt aggctgataa
     8281 ttgcccttca ttcacaagtg tgactagtgc ttttttgtca gtagcttctg ctttcttttt
     8341 cgtctgatcg attgcactag ataattcctc taattgagtt ttagcttctt cttgcttacc
     8401 aaagatctcc cctaaagcag tgacattttg ctggaaagat tcccatggat ttttcgcatc
     8461 taaagaaagg aaaatggtcg gagcaatttg acttagatct ttttgaaaat ctctttgtcg
     8521 gccagaaatg atgattagat ctggctgaag ctgattaatt ttttctaagt ctggttcttt
     8581 tattccacct gctgattcta cttttttata ggatgaaaga tactcaggaa ggctgcttgt
     8641 agctgctcca actaccttat ctccaacacc aagagcatcc atcgtatcta aagaaccatt
     8701 gtcgaatacg acgacttttt ctggattatt tggcacctcg atttggtctc cattactatc
     8761 tgttatcgtc aatgtagttt cagtttgtgt gctgcttttt tttgtgtctg cctgactggt
     8821 gttgttgcta tttccacatg ctgctaaacc aaatgatgct agaatcaaga tacctactac
     8881 taattttttc atctcttctc tcctaatgtt agttttagaa ataaagacag aatctgcggc
     8941 cttcaatctc acaaatccgt atgtccattt cgtataattc atccaataca tcttttttta
     9001 tcatttcttg ggtcgttcct tgcgtaaata actgtccatc tttcattgca acaatttcgt
     9061 ctgcataact tgcagcaaag ttgatatcat gaagaacaat aacaattgtt ttcccgattt
     9121 cttgtactaa attttttaaa atctgcatga tttgaaccgc aaaattcata tctagattgt
     9181 tcaaaggttc atccagtaaa atataatccg tgtcttgcgc tagcaccatt gcaatatacg
     9241 cacgttggag ttgaccgcct gaaagagtgt caatcgattg atccgctatt tcttctaacc
     9301 caaggtgttc cagtgattcc tgaacgattt cctcgtcttt tgtcgtcaat tttcctttcg
     9361 tataaggaaa tcgtccaaat tctactaatt ctttcacggt gatttggaca tttatttcat
     9421 tcgattgtcg taggatcgat aattttttgg ctaattcatt ggatttccaa gcctttactt
     9481 cgttatggtc taaatagatt tcacctgtat cttttggtat caaacgactg atcatcgaca
     9541 acaccgttga ctttccagca ccatttggac cgataaaagc agtcaacttc ttttcaggaa
     9601 tcgttaactg tacatcttta accactgatt ttccatggta cagtttattg accttttgta
     9661 aatcaatcat aggatactct tcctttcgcg aatcaatttt gtgacaaaat aaattcctcc
     9721 gacaaattca acgacgacac tcactgttgt attccagccg aaaatctgct ctactgcgag
     9781 ctgtcctcct gtcagaaaca gcatagctac cagaaagctt ccgataaata gctgaccatg
     9841 acgataagta gaaaagacac gataactgat atttgcgaca ataaacccta aaaaagtaac
     9901 tggacccaca agagccgttg atgtccctgt caaaagactg acacaacata aaatcaaaaa
     9961 ttcaaaacga tctgtgtcta tcccgaggtt tactgcttgt tcatttccga gatggaaaac
    10021 gtctagcaca tgtcgatatc gaaataacag gtaccagcag ccgataatga tcaaagaagc
    10081 aggcaaaaga tactgactct ggatattgcc aaagcttgca aataatctgc cttgaagcaa
    10141 atcatattca ttcggatcca tgatgacttg taaaaaggta ctgacactac caaaaagcgt
    10201 acctaaaatc atccctgaca tcagtaataa aaaaagattc gctctcattt ttttcaaaag
    10261 aaagaaaaat aacaatagac tcagcccgac catcagccca acgttcaata agaaaaaaac
    10321 tgtacctgtc tgctgaagca tcgtcactcc tccgataaag aagaaaagga ccgtttggac
    10381 aaggacatat aacgactcaa gtcccaatac tgaaggagtc aaaaactggt tatgcgtaat
    10441 tgtctgaaag ctgattgtgg cactgctgac agctagcgag acaaggataa atgcaaaaat
    10501 tttcttactc ctcaatgcac atgcaaaatg ccagtttcca tatgtatgga tcgtcaagta
    10561 cattcctgta acaaataaaa caagaacaag cataccaata aaaatagatg gctttttttt
    10621 aagcatatcg tcgtcccctt atcagaagat aaaggaagat agccccaccg atgattccaa
    10681 cgatcacact gactggtatt tcataaggaa aaatcaacac gcgagcgaca agatcgcaaa
    10741 atactaagaa caaactacct gctaaagcag ttacgccaat tgttttgtgc acttgatctc
    10801 catatattct agagacaaga ttcggaatga tcactccgat aaaaggaatg cttcctacca
    10861 tgatcaacat gactgcacta gccaatgata ctaagccaat gcctaaaaac tgcatcttct
    10921 ggtaatctat tcccagattt actgcaaacg attcgcccag accaatgatc gtaaatcggt
    10981 aagcaaatag ccaaaccaag agcaacaatg gaacaacagc atacaataac tcataatttc
    11041 ctcgtatcac agtcgcaaaa ttcccttgaa gccatgaagt catattttgt accaactgga
    11101 attgataagc aaaaaaagta gcaactgaac caatgatatt ccctaacatg actccaatca
    11161 acggaagcat caattgattt ttctggagga acaatcgcgt aagggataaa aaaagcaatg
    11221 ttcctgcaaa agcaaagcaa aatgctgtca aggatcggac aatcaaggat gcaccaggta
    11281 ggaaaatcat agccactaaa atacctaaac gcgcactatc catcgtgccc gcagtcgttg
    11341 gtgaaacaaa tttattttgg gtcaaatgtt gcatgatcaa cccacaaata ctactggtcg
    11401 ctcccacaag gatcaaacaa atcgttcgtg gcaaacgagt cactagcaag atttgtaagc
    11461 tttgcccatc taactggagc agttctgaca attgtacatt cgatactccg atgaacaatg
    11521 aaatactact taaaataact aataatgcta ctagtcgtcc gtagcgcatc caatccacct
    11581 ttctaattga gaatgatttt cattcttatt ttaagtgaat cgttgaacaa aagcaaccgc
    11641 tttttcatat gttttaggta ttattgggaa tatgctataa tcgtaagaaa gacaaagaga
    11701 agaaggaggg tattttttat ggcacaagat gatgtaaaaa gccatttgga taagggactt
    11761 tatggtacac ctttagtgaa ccctgaagaa cagcataaat atttagggac attcagggaa
    11821 cgctgctatc ttagtatgac tgttgctgaa atgatccaac caaaaaacaa agaaaacttt
    11881 ttaaaagaac tcaaaaaaca tccagatgct tccatactct tgaatggggc tctacccctt
    11941 tctgtccaaa accaatatat ccagctagca acgaaaaaca atagccgttt cactgtagtc
    12001 aacgattacg tcactgcaaa tcctgaggaa ttcggattgc tcttaacagc aaaagaagca
    12061 gtaaatgaac ctgtgattga tatcgaaaaa aaatacccag aagaaaactc ttcgccagct
    12121 gaaaaagaaa aaccaaaaaa gaaaaaaagc ttctgggatc gtttatttta aaacgaaaaa
    12181 acacataaat ttttacatgc aaaaaaagct tttgatcaga ttgacatcaa ataggacaat
    12241 ctgtcaaaag ctttttcttt taaaatactt caggatataa caatttcaat acattcatcg
    12301 tcaatcttct gccttttcct gcaaaaggat aaacatgcat atgcatggct ggattgaaat
    12361 ttgcttcgat gatcccatat gcttttttat cagttgtagg atcgatttct ttatctggta
    12421 taatcaaatc gataccacta atcttagctc ctagtgcttc taccgcagaa acggcgattt
    12481 ttttgtaagc ttcagaaaat tcatcagtca tatcgatcga atcaccacct gtactgatat
    12541 ttgagttctc ccttagataa acaatttgat tggcttgggg aactgattcg atcgtcaacc
    12601 cttgttcttt caacatcaat tgttctaatt cacctaattg gatcaattct aaaggtgcac
    12661 gatgattcgt tccacgcaaa gggtcactat ttttttcttc aacaagttct ttcactgttc
    12721 ggatactgtc accaataaca ttagcaggga ctcgcaacat gatggcttgg acttctccgt
    12781 cgatcacaaa gaaacgatat tccgttcccg gcataaattc ttctactaaa accgaactgt
    12841 cttcacgaaa agcaatggct aaacctgctt gataatcttc cagactagct ccttccttga
    12901 aaatcgtgat acctaacccg taattggttg atttaggttt gatcacaaat gcctgctcgg
    12961 caaatctcgg ataagctttt accgcttctt ccatggatga aaattctgcg cctcctggta
    13021 cccggaaccc agcttctttt aagacttttt tcgttactgt tttgttttcc atgataagtg
    13081 ggacgatata gctgtcctta cttgtcatgt ttgcattttt cacatattct acatggtctt
    13141 ggaattgcag acgcaaaaat tgatccgact catctaatac tttgacttgg acaccttttt
    13201 ggatggcatc gaacataaat atctgagtag aaagttccat ttcgcgaaaa ccggccaact
    13261 gatagggacg ttcatggctt tctttataat attgatgtcc caatgccaca gcaacttctt
    13321 gctgactcga tagctgtgct tcttgataga gtttcgctgc taacgtttgt tcaggatgat
    13381 ccatttgagt caatgcttcc tctactaaat aaagagaatc caaccaatcc aattcaacca
    13441 gcatttgctt cattccttca agaatttctc taccttcttg atgaaattct gtttgatctg
    13501 atggttgttc tagtgctact ttgttatttc gagtcgtacc ttctgctacc cattggtcac
    13561 agtcttcttt ttcttctgtc cataacatgt acatcaggaa taattgtaag aaccgaactt
    13621 gctctggatt gatacccaat cttgcataag gattcaaatc gatattccgc aattcaatat
    13681 aacgaacacc tgttgttgct aaatctgcga ctttctttcc gcctcggaaa cgaagcggtg
    13741 tataaaattc tttctcctcc gaaagtttcc cttcttcgat cattcgctcg atatctgcta
    13801 aatactgttt catagaagca taggaaactt tcacatttgg atgattcgta tagcccagcg
    13861 cactgttgcg aatactgcga acaggttctt gcggatgtga ttcatctaaa aagtagttct
    13921 tctcactcat tggtgaagca ccgaataaat aagtgatcat ccagcgatag cgcataaagt
    13981 ttctcgctgt tttcaaataa agttctgttt tgaattcgga aaaatcttga aattcttcct
    14041 gatggctgaa caatgttctt agcaactcat cgccaaattc aaaattgaaa tgaataccgc
    14101 ttaccatctg ttttcgcttc ccgtattctt ttgctaaata acgtcgatac aagacatctt
    14161 cgaaattttt taattttgca ataatgatct cttcgtcttt ttccggtaag gcaggtggca
    14221 tacttaatgg ccagatcatc tcttctttcg gtatagaacg agcagtcaca tcataaacag
    14281 cagcgagata ctgaaataat tcgggaatag aatcggtaac aggggtgatc atctctgttt
    14341 gtgtttcgct aaaatctgtt tggatatagg ggtgatagga tcgatcgcca aagattgctg
    14401 gatgatcggt ttttgctaga tttcctgcaa gatcaacccg ctgtccttcc cgttctatac
    14461 caaatcgagc ttggtcgata aaagggcgcg cattgacatg caataataat tgcttaaaat
    14521 tcatcattaa gtgacctcca attcatatga atctaattat aatcaaaaag tctaagaaaa
    14581 agaacgaaat tgtttgagaa ccgcttacaa ataagtataa aaatgaaggc atcaaaaaca
    14641 cagctatgtt aaaaacaatc attcaaacaa ggagctgcaa cttttgtcac agctcctgct
    14701 ttttagacat gggttattct tcttttagta atcctttgaa caatccaacg acaaaatcat
    14761 ttggatcaaa cggctccaag tcatcgattc cttcaccaag accgaccaat ttcactggta
    14821 aatgcaattc attacggata gcaagtacga taccgccctt tgctgtacca tctaattttg
    14881 tcaaaacaag accggtcaca tctgttgttt ctttgaattg ttttgcttga accatcgcgt
    14941 tttgaccagt ggttgcatct aatacaagaa gtgtttcttg gggtgcttct ggtaattccc
    15001 gctggattac tcgttttatt ttttctaatt cgttcatcaa gttcacttta ttttgcagtc
    15061 ggcctgcagt atcaactagt aaaacatctg cctgctctgt ctttgctctt tccaaagcat
    15121 cgaatacgac agctgctgga tcgcctcctg cattgccacg aacaacttca acaccagcgc
    15181 gttcgcccca gacaactaac tggtcgattg ctccagcgcg gaaggtatct gctgctgcca
    15241 tcaatacttt tttaccttcg tttttgaact gatgggcaag tttaccaata cttgttgttt
    15301 tccctacacc attaacaccg acaaacaaaa taactgttag tccgtcttct tggatattta
    15361 gttcatttac ttcattgatt ccttccgctt catagagatc cacaagtttc tcgatgatcg
    15421 cattttggac ttctgccgat tttttcgcat ttctgagttt cacttcttga cgtaatgctt
    15481 cggtgattcg tatcgctgtt tcaaaaccta catctgcacc gataagtgtt tcttctaatt
    15541 cttcaaaaaa gtcttcatcg actgttcgaa aattggcaaa caactcattc atccgttcac
    15601 caaatgtttt acgcgtcttt tctaaaccct tgtcgtactt ctcctgtact tcaacttctt
    15661 ctgttttctc ttcgccagta aaagcacgct tgattttatc aaaaaatccc atttgactcc
    15721 ccctactcta atacaaattt ttggatcact ttggctacgc cattttcttc attgctggct
    15781 gtcacgaaat ctgctgcttc tttgacgaaa ggcacagcat tctccatcgc tacgcctagt
    15841 ccagcatatt ggatcatcgg taaatcattt tcctcatcac cgatcgccat gatttcttct
    15901 tgtttcaaac caagatcttt tgccaataaa gaaataccat aagcttttgt aacacctttt
    15961 ggcataaatt ccaaaagcat acttcttgtt ttgatgatct catacatttc atggaattct
    16021 gctggtattt ctttgatttt aggatctaat tcttcttggt cataagcgat tactgcctta
    16081 ttaagcacaa attcatctgt aatatcgctt atatccgctg gttggaattg cagcagctta
    16141 ttcaacacat tgtacaagga ttgcttcctt ggggcactag gcagctgtaa aaccacattg
    16201 tcagacaaaa catctagtgg cagatccaat tgctgagcca gtttgtataa gcgttggata
    16261 tctgatactt ccatcacttt cttttccatg atttcaccag tatcattttt ttgtaccaaa
    16321 ccgccgttga aagtgatgct gtagtcacct tcatctacca gtcctaattc ttgcagataa
    16381 tgcgccatcg cagctaaggg gcgtcctgta catagaacga tcttgacccc ttgctgtttt
    16441 gcttgtgcta atgcttgttt attctcatct gaaatcagct tttgttcatt taataatgta
    16501 ccgtccaaat cgattgctac taatttgatc attttcgttc ctacactttc gtttcgttga
    16561 ttttcgcctc ttcattgata tcttccatcc gtacagaaac gatttttgat acaccggatt
    16621 cctgcatcgt cactccgtac agtacatctg ctgcgaccat cgttcctttg cggtgtgtga
    16681 ctacgataaa ttgtgtgtca tcttggaaat cgctcaagta acgaccaaaa cgtttcacgt
    16741 tggcttcatc taacgctgct tctacctcat ctaagataca aaacggaaca ggacagacac
    16801 ggataatgga aaagagcaac gcgatggctg taagtgctcg ttctcctcca gaaagcaaac
    16861 ttagactttg cagttttttg ccaggaggtt gtacttcaat ctcaatacct gttttcagca
    16921 aatcagttgg ttccgtcagt actaattcag ctttaccgcc gccaaacata tttggaaaga
    16981 caattttgaa ttcttgacgg atagcttcaa atacttcttt gaatcttgtc cgcacttcat
    17041 catccatctc atccatcgtt tcaaacaatt ggtttttcgc tgctaacaga tcatctcgct
    17101 gtgttgctaa gaatgtatgt ctttcactga cttgttcgta ttgttcgatt gaatttaagt
    17161 ttactggtcc cagtttttca atctgttctt ttaaggatga aacctttgtt ctactgtctt
    17221 cgatatcggt tgtttcttga tagtcttggg aagctttctc aaatgtcagc tgatattctg
    17281 actgcagata ggtcaaatga ttatctaaaa tcatttcagc tcggtctttt tggacttcta
    17341 atttggattg ttccgacagt cgtgcttttt gctcacgatt acgctctgcc aagactgctt
    17401 caagcttatc gatctccttt tgccttctat ctcgctgctc tttggctttt gccaactcgg
    17461 cttttaatgt ttctcgctga gcagataatt cgttaatttg tttttccaaa ctttcttctg
    17521 tgacttcatg atcagaaaag tctgctgtca atgtcgccaa ctgcttttct atcgcttctt
    17581 gacgttcggt tgcctctgct ttttggactc gggcaccgcg gagctggatc tgcagatgat
    17641 taaactgctc tttcaagaca gcaagatctg cttgttcttg tgctttttga ctttgtactt
    17701 gtgctcttct ggcttccatc tggtcgcttt cttgacttaa cgatttgatt tcttcgtcga
    17761 ttttttgtcg ttgcgattca agatctgttt gtttgtcttt caattcgttc gtttgctttt
    17821 cgtaatcttc gataaactgc tgcagttcac gtgtctcaaa agttgagatt tgcttttctt
    17881 tttcaaaacg ttcaagttca tttatgatgt tttgtagctg atttgtcgct tcttgttctt
    17941 caaaacgtaa ttgttcgcct cgtgtacgca aaacttcttg ttcttcggct aaacgagctg
    18001 tttcttgttg cagctcctga actttttttt cttgtgcttg gagttgttta tctgcttctt
    18061 caaattcact agtcaactgt ttcaattctt gattttgtac aaacaaactg ccttggtttc
    18121 ctcgtttatt cgctccacct gtcatggatc ctccggcatt catcacatca ccttctaagg
    18181 aaaccactcg atattgatag cggatcgttt gcgcgattgc attggcacta gttaagtctt
    18241 tggctaaaag gatcgttcct agcaggtttt gaaccacagt ttggatttga tcgggataac
    18301 tgacttgttc actggcaatc cctataaacc cttctacagc tgctgcttgt gtcaatatat
    18361 gagccggtaa ttgtcttggt ttgattgtcg tcagtggaag gaatgttgct cttcctcctc
    18421 tttgctgttt caaatacgtg atggcttgcc gagcatcttt ttcattttca acgatgacat
    18481 gctgggctgc cccacctaaa gctgtttcga tagctaacgt gaaatctgca ggaacatcga
    18541 tcagttcagc cactgctccg acaatacccg aaagctgctg tttatgctgc aagaccaatc
    18601 gaaccccttg gtaaaaacca aaatagtttt cttggatttc ttgaaggctt ttcttacgag
    18661 cacgtagctg ctggacttga ttcattaatt gatacatttt aggctgttct tttgctaatg
    18721 cttcttggaa ctttttcccg ttttgttgga tcatttctaa cttctctttt gtttcagtta
    18781 aagatgattt tagctgcgcc tgcttttgtg tcaattcttc tttttgtaag atcaatcggt
    18841 ctactgatgc ttctacttcc gattgcttgg ctaacgtctg cttgcttttt gctgtttctt
    18901 gtatatattg acgttctaga tacttcagtt cgtttcctac agctgcttgt tcttgcatca
    18961 aatcaacata ttgatctctt aattctgcca ataattcttt cgttgatttt tggtattttt
    19021 ctaattcttg ctgtgttttt atgagatttg cttcagcttt ttggatctct gtttcttttt
    19081 ctgctgctgc tttcatcaag ctttcttgaa gcttttcaaa atgtttaacc tttttttgtg
    19141 cttctgctaa tgaagtttga tattcttgcg agcttttttg tgtatgcttt gtccgctcct
    19201 gcaaaacatc tttttgtcct tctgtctgct tcagtttttc cgaaagatct aataatactt
    19261 gctgattttt ttcgatcaga cgatcggctt gcgcattttc tttacgctgc ttcgccaaaa
    19321 tggattcttg ttcttggatc gactcagata gtttgcctaa ttctagattg aatttcgcaa
    19381 gctgtgcttg tttgttgtcc caatcttttt ttgctgtctt gatttcagca accatcaaag
    19441 agacatcggt ttgagtcagt gtttctttta gccgtaaaaa ctcctttgct gcctcactct
    19501 gggcggccaa aggagttaac tgttcttcca attcgtggat gatgtcttgg acgcggctga
    19561 ggttgtcttc tgtttcaaaa agcttctgtt ctgctttctt tttccgctgc ttgtatttca
    19621 agacaccagc tgcttcttca aagatcccac gacggtcttc cggtttacta ctgaaaatcg
    19681 cttctacttt tccttgagaa atgattgaga acgattcttt tcctaatcca gaatctaaga
    19741 acaattcttg gatatctttt aatctgcagc tttgtttatt gatgaaaaac tcgctttccc
    19801 cagtccgtcg ataacggcgg gttacactga tctcattgta ctccaatggc agataatgat
    19861 cactattgtc taaaatcact gtgacttctg caatattcag ctgcttacgg gtatcggaac
    19921 cggcaaagat gatatccggc atttttccgc ctcgcagact tttagcagat tgttctccta
    19981 atacccagcg gatagcctct gtaatattgc tttttccgct gccatttggt ccaaccactg
    20041 ctgtgacgct attttcaaaa tcaatgactg tcttgtctgc aaatgattta aaaccggcta
    20101 tttcaattcg tttcaaatac aaagctttcc ctcacttact tttacagtgc tgccaatgca
    20161 ttttccgccg cagcttgttc tgctaatttt ttcgatttcc cttgtccagt gccaatcaat
    20221 tgatcatcta cataaacttc gatccaaaat actcgttcat gagctggacc ttcttcattg
    20281 atcaatcggt attcgattga tacatcacct gatttttgca agacttcttg tagttttgtt
    20341 ttatgatcca tctcatgtga aaaagcacct gctttgactt taggaaaaat cactttttcg
    20401 atgaatgcat gggtcgtgtc gaatccctgg tcaagatata acgcgcctaa aaaagcttcg
    20461 aataggtcgc aaagcaatgc tggtcgcgta cgtcctcctg aattctcttc tcccttgccc
    20521 aacaaaacat attgatcaaa gtgacattct ttggcaaatt tcgacaagct gtcttcgcga
    20581 acgatcgcag ctcgtgtttt tgtcaatttt ccttctggca aatgaggata taaacggaat
    20641 aaatactgag aaaccatcag ttctaaaacg gcatctccta aaaattcaag tcgctcatta
    20701 tccgataatt gcagattgcg atgctcattc acataggatg aatgggtaaa agcttgttca
    20761 agcaaattta gatcgtgaaa aacgatgtta tagttttctt ttagttcttt tgtcagctgg
    20821 ttgtccattt tccacattcc ttttatggat attttttcga tacttcctta ttatactggt
    20881 tttttgtcaa atttgaaatg gttcggtcaa aaggtaagta aagctttaat aatcctagtg
    20941 attgacgaga agttgtgaaa aattcgttta gtttcgagta tgaagaaaaa attttgtgaa
    21001 aatggctttc catatttttg cataaatttg acttaatacc gagaaactgg actttgaaca
    21061 ccgtttattg gagaagtcat gaaatcagcg ccttgacctg aatattagag aaataaacag
    21121 aaaaatatca aaaaaccgaa aaaggacatc gccctttttc ggtcattttt agtgattatg
    21181 aacgtgaatc tgctgtaaca gaaacatttg cccatgggtt tggtacatca taagaccaat
    21241 cccagtcttt gacacggtcg ttgactggca atacttcatt acggaacaac gttggaatcg
    21301 caaatgcttc ttcaaatgca tattgttgcc atttttgata tgcttctttt tggtactctt
    21361 catcaaatgc tttgcttgaa ctgatatcgt tgatcaattt tgtattttct tctgattcat
    21421 aacgagtgta gttgaacgca gcatttggtc cccatagtcc acttggattt ggatcagttc
    21481 ctgtattcca agcagcttgg taaacatcaa tttcaggatc atcgttttct aatttatcat
    21541 agaaagaatt gaattcgatc agacgacctg ttgtgtattg gacatccaaa ccaatttctt
    21601 tccattgttg gatatagtaa tctgccaatg gttgcgctgt ttccccaccg gacatcgaag
    21661 caaagttgat tgttagttta tttccatctt tgtcttctct taaaccatcg tcatccacat
    21721 ctttgtagcc agcatcatct aatagttttt tcgctttgtc tagatcttgt gtgaatcctt
    21781 tcatctcatc atcatggtat gactggaata ctggaggaat caatgaagtc gcattgcttc
    21841 tcaaacctgc atagaaacgt tcacctactg cattattatc taatgcatag cccattgctt
    21901 gacgtaatga tttatcagcc atttttgaat ctggattgta ttccacactc tttgtatctg
    21961 cattccattt acctaacttg aatcctagat aagtataaga agtttgttca cggcctaagt
    22021 ttgtatagcc agcaatttct ttataggtat cgtaagtatc tgttggcatt tggaaaatca
    22081 tatcatataa tttagagttc aatgcttcag ttgcacttgc catcggtaca gatttcatcg
    22141 tgatcttttt caatttaggc gtaccattcc agtagtgctc gtttggcaag tattctactg
    22201 attctcctgc aacgatatta ctcatgtaat aagggccaaa agtcactgga tttttacgta
    22261 cttgatcgct tgattccata tctttgattg ctacaccttc taacgtatgt tttggtgctg
    22321 catatgacca gataccacca cccgattgaa gcatgcttgg gtttacttct ttatattcga
    22381 tttcaactgt tttgtcgtct atttttttga ttccggagat tgtgtcggca cttccatcat
    22441 gatattcttc catacctacg atgttacgga aagtgtcgtc ataacggata ccagtgtaat
    22501 ctttattccc aatgatttcg taagggaaaa tcacatcgtc cgccgttaca tcttcaccat
    22561 ctgaccattt gacgttgtct tttactttga ttgtcgcttt tttcgtatct tcatctagat
    22621 caagcgttgc agctccgtcg tctgtgattc tgaaatcgtc atcgtttgca aatagcggtt
    22681 catgagaagg agccataaaa gctgcatcat atgaatcttg gtaaaactcc cactggaaca
    22741 atcctttaaa ctgactgtcc gttgcgaccg cagcttctaa ttcgccgtct tcgattgcat
    22801 catcattatt ttttgttgca attggaaaag ttaattcctc tttgttattg ccgttgcctc
    22861 cagagttgtt tccagaggta tctgtacctc cgcccccgca tgctgcaaga acgacagcag
    22921 acatcagcgc tgcaaatcca aaaatcgttt tcttcattcc tatttccccc ttcgcttcta
    22981 tactagccta accgttggcg cgcatctgca gaacgcttga atgcttgtcc aacataattg
    23041 atactcaaca tcaaaactaa aataaggatt gatgcgggta accagatcca ttgccgattc
    23101 actaagactt ctccgttact tgcataaccg atcaatgtac ctaaactagg tacatttgcc
    23161 ggcaagccga aacctaagaa agtcaaagtt gtctcgatac cgatgtttgc cgcaaagttc
    23221 atcgtcaaat tagtaatgat taatgaactt agattcggca tgatttcgcg gaacatgatt
    23281 ttaaaatcat tggttcccat tgtttttgag gcactgacat aatctcttct tgcttcagat
    23341 aatgtcttgc ttcggaataa tcgggcttta gctacccaat aaaatgcact catgatgaat
    23401 ataaaggacc agacattgta tctggaaata atcgatacga agacgataat gatcagcatg
    23461 atcggtagaa tcatgataaa atcaacgatc cgcatcaaga tgttgtcaat gatccctccg
    23521 tagtagccag agatgatccc taatccaacc ccgatgatcg aagtaaggat cgtgatcgca
    23581 aatccgatcg taacagagtt tctagcacct atgatcaatt gtcctaatac atcacgtccg
    23641 ccttcatctg ctcccagaat gaacttttgt ccttgtgtcg aaactgttcc tggtgcagcg
    23701 tatttgtcga agatactaac atacatcact ttgtcttgat cggtcagcat cgcaccgata
    23761 aagatcacta ataataaaac cactagcaaa cctaatgaaa aaatagcaat tttatctttt
    23821 ttaaattctc gagcaatcat tcgtatcccc ataggaggaa tactttcttg aactgctgta
    23881 ttgttttttt cactcatcga tccattctcc tttctgattg gttttattga atccgaatac
    23941 gcgggtcaac gatactcatg atgatatctg aaagtaatgt ccccactagc gtcgcgattc
    24001 ctaaaatcaa tactaaggat gtgatcacgg tatagtcccg ttggccgatg gaatcaataa
    24061 atagttttcc gactcccgga taaccaaaaa tcttttcgat aactacagaa ccagcaatca
    24121 acgaagtgat ttcatacccc atttgagaag ccatcggcaa cgctgcattt cggaaaatat
    24181 gtcgactgaa tactttattc gtcggtaccc ctttggaacg ggctgttctg acaaaatcga
    24241 gcgatttcga atcgattact tcacttcgca gatattggat tgtgacggca gtcgccaaaa
    24301 atgcttgggt aattgatggt aacagaatat atttcaaacg gtctatccat tgagtgataa
    24361 atccaccttg caaacctagt gtttgtgatc cacttgttgg gaaccagcct aaacgataac
    24421 caaaaacaaa tagcatgatc aagccgaaga taaataaagg aacagcaaaa ctaaagaagt
    24481 tgtagattac gacaatctta tccaagattg aattttgata ccggccagca agcatcccca
    24541 gaggcacagc aatcaaataa gtaaggacaa ctgtcaagat cgatagatat aaagtgttga
    24601 tgatccgttc gccgatcaag tcagcaacag agcgtttgta taagaaactt tgaccaaaat
    24661 cgccttgcaa agcatttccg atccaacgga agtactggac ataccatggg tcattcaagc
    24721 cagctgcttg tcgcatctgt tcgatcactg ccggatcctg atttggattg atcaaaccgg
    24781 taaatggatc ccccggcatc gcttgggcta ataagaagat cagaacactt aaaataataa
    24841 cttgcgggat catcaataaa acacgtcgca gaatcgtttt ccacattagc ttgcacctcc
    24901 atctttcaac gccactttgt gtgtagttgt gatcgtacgc aaatcgtaga ctcgaccagt
    24961 cgcatcatag tattcttttt ggttttggat atactcttgt tctactcttc gtcgattttc
    25021 tttgtggctt tccctatttt ctacatcgat ttgcggaata gctgaaagca gacgtttcgt
    25081 atagatatgt cgcgggtcat tataaatgtc ttctcttgta ccgatttcta caaatcgacc
    25141 tttgtacata attgcgatat tgtcgcacat gtgttttact acacctaaat catgtgaaat
    25201 gaataaatag ctgaggccgt attcttgctg gattcgtttc atgaaattca atacttgcgc
    25261 ttgaacagat agatccagag cagatacagg ttcatcggct acgatcagtt ttggattggt
    25321 agcaacagca cgggctactc ctagacgttg acgttgtccg cctgaaaatt catgaggata
    25381 tttatatagc gcatcttccg gcatcccaac gatatctagc agtcctttta cctttttctt
    25441 ttcttcttga tcgcttaacc gttcaaaatt tcggatgggt tcagcaatga tatccaacac
    25501 tcgcttttta ggattcaaac tggacattga atcttggaag atcatctgca catccttgtt
    25561 ataacccatt tttttgcggt tgcttcgctt cgttacatct tttccttcat acatgatttg
    25621 tccggctgtt accttttcta atcccacaac ggcttttccc gtagtagatt ttccagagcc
    25681 agactctccc actaaaccat atgtctttcc tttttcgata ataaaatcca caccatctac
    25741 agcatataca tgatccgtta ctcgattgaa gaatccactg cgaatagggt aatgaacttt
    25801 caggtctttg attgtgatta attctgtcat ctacacctct cctttttcgt ctctaaagtg
    25861 gaaatgctta tagcaggagc aacgtaccaa gtggttcggt gcgacttcat gaaggatagg
    25921 ctcttcttca tgatcgcttg ctggtatcca tggaatacgt ggtgcaaacc gacaaccttt
    25981 acgaggcatg tttttcagag aaggaacgac accttcgatc acatggagtt ctgcatcatg
    26041 atttccttct tgtggtatag agttcagtaa agagcgtgta taaggatgct taggatgatc
    26101 aaacagttct tttacacctg ctacttcaac gaactgtccg ccatacatca ctgccactcg
    26161 atcagccatt tctgctacga ctcctaagtc atgagtgatc aagatgatcc ctgtttttgt
    26221 ttcttcctga aggtctttca gtaaatcaag aatctgtgct tggatggtta catctagggc
    26281 tgtcgtcggt tcatctgcaa tgacgattgg aggtttgcag gcgattgcga ttgcgatgat
    26341 gacacgttgt ctcataccgc ctgatagttc atggggatat tggcgtccaa ctctcgcagg
    26401 attaggaatc cctacttgtt ctagcaattc tagtgcacga gcttgacgct gttctgcatt
    26461 catctttgtg tgataggaaa gtccttcttt gatctggtct tcgattctca tgagaggatt
    26521 taatgcagac aacggatctt ggaaaatcat tccgatatca tttccgcgga ttttattata
    26581 gagcgtttcg ttcaggtcga ttaaattcag atcgttatat aaaatctcac ctgtaatttt
    26641 tgtgttatta ggatcaagta atcccataat cgtagtagcc aaggtacttt tcccgcaccc
    26701 agattcccca acgattgcta aaatctcgtt tttgtctaaa gtaaatgaaa cgtcatctac
    26761 tgcatcgtaa tagtcatctt tgagtcggaa tcctgtatgt aggttctgaa cttctaataa
    26821 ttggttatcg gacaatcgaa tacccctctc tgcttcctaa atggaagttt ttcttaataa
    26881 aaaaggacat tacacagaat aaatcgtttt ataacaaagg ttattctta
//
LOCUS       DAYYHB010000033        26491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000033 DAYYHB010000000
VERSION     DAYYHB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.034"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..844
                     /locus_tag="ACHA6B_001329"
     CDS             <1..844
                     /locus_tag="ACHA6B_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8948913.1"
                     /translation="NKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLRSLK
                     HEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSF
                     IGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIE
                     NVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKT
                     YKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQS
                     KAPF"
     gene            856..1125
                     /locus_tag="ACHA6B_001330"
     CDS             856..1125
                     /locus_tag="ACHA6B_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948914.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1130..1294
                     /locus_tag="ACHA6B_001331"
     CDS             1130..1294
                     /locus_tag="ACHA6B_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948915.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1287..1589
                     /locus_tag="ACHA6B_001332"
     CDS             1287..1589
                     /locus_tag="ACHA6B_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948916.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1586..1747
                     /locus_tag="ACHA6B_001333"
     CDS             1586..1747
                     /locus_tag="ACHA6B_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8948917.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1744..2049
                     /locus_tag="ACHA6B_001334"
     CDS             1744..2049
                     /locus_tag="ACHA6B_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948918.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2049..2405
                     /locus_tag="ACHA6B_001335"
     CDS             2049..2405
                     /locus_tag="ACHA6B_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8948919.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2365..2610
                     /locus_tag="ACHA6B_001336"
     CDS             2365..2610
                     /locus_tag="ACHA6B_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948920.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2607..3026
                     /locus_tag="ACHA6B_001337"
     CDS             2607..3026
                     /locus_tag="ACHA6B_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8948921.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3023..3580
                     /locus_tag="ACHA6B_001338"
     CDS             3023..3580
                     /locus_tag="ACHA6B_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8948922.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3577..3873
                     /locus_tag="ACHA6B_001339"
     CDS             3577..3873
                     /locus_tag="ACHA6B_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948923.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3950..4363
                     /locus_tag="ACHA6B_001340"
     CDS             3950..4363
                     /locus_tag="ACHA6B_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8948924.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            4672..4824
                     /locus_tag="ACHA6B_001341"
     CDS             4672..4824
                     /locus_tag="ACHA6B_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948925.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(4821..5096)
                     /locus_tag="ACHA6B_001342"
     CDS             complement(4821..5096)
                     /locus_tag="ACHA6B_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948926.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5592..5756
                     /locus_tag="ACHA6B_001343"
     CDS             5592..5756
                     /locus_tag="ACHA6B_001343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948927.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            5952..6119
                     /locus_tag="ACHA6B_001344"
     CDS             5952..6119
                     /locus_tag="ACHA6B_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8948928.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6145..6489
                     /locus_tag="ACHA6B_001345"
     CDS             6145..6489
                     /locus_tag="ACHA6B_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8948929.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6494..6775
                     /locus_tag="ACHA6B_001346"
     CDS             6494..6775
                     /locus_tag="ACHA6B_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948930.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6878..7192
                     /locus_tag="ACHA6B_001347"
     CDS             6878..7192
                     /locus_tag="ACHA6B_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8948931.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            7170..8864
                     /locus_tag="ACHA6B_001348"
     CDS             7170..8864
                     /locus_tag="ACHA6B_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8948932.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8884..10062
                     /locus_tag="ACHA6B_001349"
     CDS             8884..10062
                     /locus_tag="ACHA6B_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8948933.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            10025..10711
                     /locus_tag="ACHA6B_001350"
     CDS             10025..10711
                     /locus_tag="ACHA6B_001350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8948934.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10711..11871
                     /locus_tag="ACHA6B_001351"
     CDS             10711..11871
                     /locus_tag="ACHA6B_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8948935.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11881..12756
                     /locus_tag="ACHA6B_001352"
     CDS             11881..12756
                     /locus_tag="ACHA6B_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948936.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12753..13064
                     /locus_tag="ACHA6B_001353"
     CDS             12753..13064
                     /locus_tag="ACHA6B_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948937.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            13054..13407
                     /locus_tag="ACHA6B_001354"
     CDS             13054..13407
                     /locus_tag="ACHA6B_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8948938.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            13397..13798
                     /locus_tag="ACHA6B_001355"
     CDS             13397..13798
                     /locus_tag="ACHA6B_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948939.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13791..14195
                     /locus_tag="ACHA6B_001356"
     CDS             13791..14195
                     /locus_tag="ACHA6B_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948940.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            14207..14815
                     /locus_tag="ACHA6B_001357"
     CDS             14207..14815
                     /locus_tag="ACHA6B_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8948941.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14835..15197
                     /locus_tag="ACHA6B_001358"
     CDS             14835..15197
                     /locus_tag="ACHA6B_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948942.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            15200..15382
                     /locus_tag="ACHA6B_001359"
     CDS             15200..15382
                     /locus_tag="ACHA6B_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948943.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            15399..18830
                     /locus_tag="ACHA6B_001360"
     CDS             15399..18830
                     /locus_tag="ACHA6B_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8948944.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            18881..19618
                     /locus_tag="ACHA6B_001361"
     CDS             18881..19618
                     /locus_tag="ACHA6B_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8948945.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19628..21919
                     /locus_tag="ACHA6B_001362"
     CDS             19628..21919
                     /locus_tag="ACHA6B_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8948946.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21943..24069
                     /locus_tag="ACHA6B_001363"
     CDS             21943..24069
                     /locus_tag="ACHA6B_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948947.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            24086..24235
                     /locus_tag="ACHA6B_001364"
     CDS             24086..24235
                     /locus_tag="ACHA6B_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948948.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            24232..24678
                     /locus_tag="ACHA6B_001365"
     CDS             24232..24678
                     /locus_tag="ACHA6B_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948949.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24680..24817
                     /locus_tag="ACHA6B_001366"
     CDS             24680..24817
                     /locus_tag="ACHA6B_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8948950.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            24855..25148
                     /locus_tag="ACHA6B_001367"
     CDS             24855..25148
                     /locus_tag="ACHA6B_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948951.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            25145..25369
                     /locus_tag="ACHA6B_001368"
     CDS             25145..25369
                     /locus_tag="ACHA6B_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8948952.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            25366..26391
                     /locus_tag="ACHA6B_001369"
     CDS             25366..26391
                     /locus_tag="ACHA6B_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8948953.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         9413 a         4011 c         5624 g         7443 t
ORIGIN      
        1 caacaaacga tactactatt taaaactaaa agagaacttt ttcgatagcg acgagatggt
       61 tcttttagaa agtatgccag atggatacat ttattctaat attcttctca aactttattt
      121 aagaagtctg aaacatgaag gcaaactaat gtttaatgac aggattccat tcaattctac
      181 aatgcttgcg actattacaa gacactctgt gggagttgta gaaaaagcag tacaaatatt
      241 tcgtgattta caacttattg aggtattaga taacggagca atttatatgt ctgatataca
      301 aagttttatt gggaaatctt caactgaagc tgataggaag agaaaataca gaaaagaaat
      361 tgaggaagca aaacagaatt taataacaag tggacaaatg tcggacaaat gtccggacaa
      421 aactacacca gagttagaga tagagttaga gaaagatata gagttagaga aagatataga
      481 gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc cgtcataaat acggagagta
      541 taaaaatgtt cttttgtcag atgagcagat ggagaaactc aaaatagaat tccctaatga
      601 ctaccaagag cgaatagaac ggctatctga gtattgtgaa tcatctggta agacttataa
      661 aaactatttg gcaactattc gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc
      721 aagcagtgga tacaagcgca caggaagacg agagaagctt cctgagtggg caatcgacca
      781 agaagcctat ctcaagaaaa aagcgctaga acgagctaat agacaatcaa aagcaccatt
      841 ctaagaggtg ggaaattgaa aatcgattat ctagaactaa ttaatgaaat agcaaagtat
      901 aaaactggtg aggaaataga aatcctgaga gacgtatatg atcaactcga agaagctgga
      961 atcgaaggaa ttaagaatga tcgttcgagt tggagtaagc tcagatacta tttcgcactt
     1021 tatatcgata caacacaatt aagaaattta gcatatacca aattactatt tgttgattgt
     1081 gttaaaggct tgcaaaaaca tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa
     1141 gacatttaca gcacagattg aactaatgca tcaagaagct ttaagacaaa gtgtatcgta
     1201 cgaagacaag tggctcaaca cgttccacgg cggacgtgag agcgcacttg atcaagtact
     1261 caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg
     1321 aattgactta ttcagaagat tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc
     1381 ttggaagagc gatgtgggca gacaagagca acaagccaag accacgaaaa atcgcaattt
     1441 ggcacggtga caaacttcta gtgataggga cagctgaaca gttagcaagt ctcacaggct
     1501 tgcacgagaa aatcgtgagg aaaagagcaa ggtgtggcta cacagacgct aagaagagaa
     1561 cgtttagata cgtggaggaa tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg
     1621 aagccttcag cgtgagattg acgatctgga acgaacaaag gcagtgatgg tcaatgaaac
     1681 ggcgagaaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc atcgattgga
     1741 ggaatgagcg tggataagaa agcagcaatg aaaagaattg ctgaattaac caagtcagaa
     1801 tcttggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg
     1861 actgaaaagc ccaaacggag aacgccgaga aaaattgcaa tctggcatga tgaccgaatt
     1921 ttagtaacag gtactgctga acagttatct gaaattactg gattaagcaa aaacattatc
     1981 tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag gtatgtggag
     2041 gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa atcatgatga
     2101 agattcagaa agataaaaaa tcaaaagaac gagctgaatt agttaagtta gaaatggctg
     2161 aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac attgaatttt
     2221 attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg caaacaaatt
     2281 ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa gaaatattag
     2341 aagagtattt gaggaggaca gcaaatgata aaaaaactcg ttcaattcag catggattta
     2401 tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt
     2461 ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt
     2521 catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
     2581 attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg gataaacgaa
     2641 agaacgtaat gagagatgta gccgtcttgc attttactaa aaacggcaaa acaaacttta
     2701 ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca
     2761 tgcaatccac aggaatgaaa gataagaatg gtgtggatat atttgaaggg gatatagtat
     2821 tagtcagcgt gcgaaatggc ttcgattact tagataataa agtctgtatt gtcaaaaatt
     2881 caatagatta ttccggatta gtttgtgcca ctgttgatga agacttagag tatcaaattt
     2941 ttaacacaga gctgtttgaa gaatacacgt acgaagtcac cggaaatata tacgagaata
     3001 gcgagttatt ggaggaacag cgatgaataa acaggaagca atcaaaaagt tagaaagtat
     3061 taaagcgata ggaaatgatg caatagctgc ttgctataac gagagtataa attcaggtat
     3121 tacgttaatg aaaaaaatag acgaaccgca gaaaccagtt attccacaac tcgtggccgg
     3181 ttggcttgag aaatctacgg acccttttac aaaagctgaa aaaatagcgt atttaatcaa
     3241 atctaaagat ggtgattcat attatttctg tgattggttt gtacgagatg gcatagtgac
     3301 gcaagagcaa ggagaagaat tactcgcttg ggcaacaaga caatcatatg aaacactatt
     3361 gagcctatac aacggctacg aggttgagaa agagccgtta tgggcaataa agaatgccga
     3421 tggaaactat cttactaaat gtgctttatg gggaaaagat ggagtaaatt atagttttga
     3481 atgcaatcca tctcatcgat tgcttttcac tgataaagca acagcggatg ctgcagcatt
     3541 gttggtgaat ggaacagtgg aagaggtggt agaaagatga aactaaaaga cggattttac
     3601 gctagcagtc acggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca
     3661 cgcaagaaat caaaattcaa agtcggtgac atggtccgct gtgaagcaga agagttcgtt
     3721 tatccgttca gaggatacgt taaaaagata ctgtcaaact cagcaatcat tcgtattgaa
     3781 aatacaatga agtgtgatcg ttcgactgca aaacacaagc attatttagc tgtagcgaga
     3841 ttagttgaca tggaagtaat caagagcaaa taaaaaaagc cggatcgctc cgactgattc
     3901 aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt
     3961 acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg
     4021 gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga ttataacgga
     4081 tatgccgaag gcaccgaagc atggcaataa ggcagaagac gcgattattc agatgatgga
     4141 tatagaaacg gagagagatg cgattctagc ggctttgatg gctcttagtc tgattagtcg
     4201 tcagatactc tactacagct tctgtgtgcc agatagcttc tcaaactaca gaattagccg
     4261 tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc taatagagtt
     4321 tgcagaagca tataaacacg gaaaaataat tgcttataaa taatttggcg gttttttggc
     4381 ggaatgatgg cggtttttag acatttacca gtgttattat ggtagtgtcg aaagattagg
     4441 aaacaggatc gacaaaataa acgaagggag gcaatctccc tcatcgtttt aaacttcttc
     4501 ttagatagac agcaactaat tatgaagaaa aggatgtgaa ttcaactcct cctaaattct
     4561 tcatcggttg ctgtttattt tgattagaca gcagcactca caaaactaaa catgttcttg
     4621 tcgtctttca actgctgctg tctattaatt tatgtattgg agggaagaaa aatgaaatta
     4681 tcagtagaag gcactccaga agaaatacaa gaattgctcc aagctattgg tagtagcaag
     4741 gagcaaaagg taagtgtttc taattcagat attgacggta tttgcaattg tttaaacaga
     4801 gaaaaaatat ctaatcctta ctaagagaaa atatgttcgt taatgaattt ccaattttcc
     4861 tctgtagctc taccttgata atttttttgc agttcacaga tgaataagga atcgttatta
     4921 tcaagtactg tatttaattt ttcacacatt tgatttggat tcaaattagt tttaactaac
     4981 caaaaagatt tttgtagctt tatatatgcc ccaaattctt ttattatttc aaatacttta
     5041 tcatactttt gccctgggtt attaagatca taactaatca tgtagggttt atacatgttg
     5101 tcttgcacat ccttgtttta tatttcagcg gaccactcgc tgataaataa aattatacgc
     5161 ttagtattta ttttcacaat attaatttat cgtaataact tttgagtaaa tagaacaaaa
     5221 aacctgcact agtttccgct agtacaggca gtgactatat cggttgataa tctagcatat
     5281 attaaaaata attgcaagaa aagaaattta tagtggttta taccaaatta tcaaagcaat
     5341 gttattttgt tgctgtctat tgttttttaa ttattcacac gataactaaa ggtgggtgaa
     5401 gagaaatgat tccattaata atttcaattt ttgcgctctg tcttaatgtc tatatgattg
     5461 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
     5521 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa
     5581 ggttcgattc cttgccagcg acattaaatg cctatgacgg ttacgactac cgaaaaaaga
     5641 tcgttaagaa gctatacggt gctacgtacg gcaatgtagt aagtgtgcta tctgtacacc
     5701 accaagcttc ggtcactgtg gcggaagtag aagacgcagc ggtaaatggc gagtagcctc
     5761 gtgagagcct ggtaagttct cgtgagtggt gcaatcccac tccagcgact ttaagcaacc
     5821 gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt
     5881 gcaagttact attacatatt agatcactct ttgagtggtc tttttatttt tgcacaaagg
     5941 aggaaacaac aatgtataga ccacaatact tagaacagaa gtatgaagta atcactgttc
     6001 ataaaggtaa tggcgaaaaa gtatatgagt atagaagacc aataaagagc gatacatata
     6061 aacgaaagga aaacaatgaa gttattccat tgtatggcaa aagaatagct aagcattaaa
     6121 taagattgcg aaaggagacg gaacatgacc gaggaattct atagatggct attacagttg
     6181 acaagagaag atcgtttggt taagttctat cagtctccta aatggcgcag gcttagagag
     6241 aaagcgatga aacgagatca ctatgaatgc caagagtgta gaagactagg taagtatcat
     6301 agagtagaga acgttcatca tataaaggaa gtcaaggata gacctgactt agctttagat
     6361 ttagataatc ttatttgttt atgtgttgaa catcataatg aagttcatgg cagatatctt
     6421 acagcattag ataaacaaga gaagaagata gaaagctttg ctaacttcga tgcaagtgaa
     6481 aggtggtaag tgcatgatca tcaatgataa tggcagagag tatgatacag aaaagattga
     6541 agagtattca tcttatactc agggattaat taaacgtttg atatacgttc gctatgtagg
     6601 tattagggat ctgttatcag ataactgttg tagtaaatac aaagtgaatc aagtaagaga
     6661 agcgttgaat aaagataata acgtcgaaag aataaaaaat gtttttggat atagtattga
     6721 agagattaat tattacattg acttcgctga agctttcatt ccgatggtga gataaccccc
     6781 ccttaaaata aatcgcaaat tttttggggg tgatgaaacg gagggggctg tcaggaaaag
     6841 agattttttc gaactttatc atgaaaggag ggctaaaatg tttaaaaacg aattgtctca
     6901 aaatcgctac agagaaaaat tacgccgctc tttaataagc caattggaaa gtcagaaaac
     6961 aaatattgag ccattcttag ataatgttga tcgttatatc agtttatggg aaacggcgat
     7021 atcactggaa gaagatatat ccgagaacgg cattagactg gagaatggta aaaagaatga
     7081 atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc
     7141 cattactcct gaattggtag gtgaagcaaa tgaatcaatt cctgagttat aagcatattg
     7201 aaaattggtt caaagctata gaagaaggca ctatcaaggt atgcaaagag caattattgc
     7261 taaaaaatta tctagaagaa agagtcttta ctagagaaga tatttacttc gataagcaga
     7321 tggtagagga ttcaatcaat ataccagcac aatactttcc attcgaatta attccgtggg
     7381 aaaaatttct acaatgtttt atttatggtg ttcgatggaa aaaagataaa acactagtgt
     7441 tcaatagata tctttcatta atgggacgtg gtaatggtaa aactggtttt gcttcttgga
     7501 acaacttctt tctactaacc gctaaacacg gtattaaaaa ttatgatatt gatatctatg
     7561 ccaataatga aagccaagca aagactagtt ttgatgatgt atttaaagta attaaagatc
     7621 atcctgattt agataaaaaa gtatttaaag ctacgaagga agttattcaa aatatcgcta
     7681 caaacagcaa acttcgttat aacacggcaa atgctagaac aaaagatggg aagcgaccag
     7741 gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta ttcaatgata aatgtggcta
     7801 cttctggtgg tggtaaaatt cgagattata gagaatttta tgatacaact aatggtcatg
     7861 ttcgtggtgg tccgcttgat gacattatag aagaatcaaa aatgattctt tctggagaac
     7921 ttggaattga caaggatgga gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg
     7981 ataacgataa tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca actattaatt
     8041 acaatgcaga tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg
     8101 gtttaagact tacgttcatg accaaacgaa tgaacagacc tatggaagat acacgatttg
     8161 ctgttgcttc atatgatgat gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg
     8221 atgaagtgat aggaacagtc gattttgctg atagacgaga ttttgccagc gttgggttgc
     8281 taggaaaata cgataaagat gtttatttta cacaacatac ttttatccac gaatcagccc
     8341 ttcgattaca aaacatcaaa cgagaggtta tagatatttc tatagatcaa ggaaaatcac
     8401 agatcgttca tggaaaaaat atagaagctg attatattgt aggttggttt cttgaaatga
     8461 gtaataaata ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc
     8521 ccgctttaga agaagcaggt tttactgtgg aaattgttcg aagcggatct gttacacatg
     8581 gtatgttaaa agatctggtt gatgaccttt ttattaatca acgtttattt tttggtgacg
     8641 atgcgattat gcgttggtat tgcatgaatg tatatgaaga gcatatttct aatggaaata
     8701 tacgctatga aaaaatagaa cctgaaacta gaaaaacgga tggctttttt tcattccttc
     8761 atggtttgaa ttttttagat gatatttatg attctgctcc tgtaacagtc acaaatagct
     8821 cagtagaaaa tacaggaact ggatttactc ctctagtatt ctaacttgaa aggaggtgag
     8881 aaagtgggga tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac ggttccttta
     8941 gaagaatact tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc gattgaaact
     9001 tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata tgaagatgga
     9061 aaaaataaaa agaatgatct ttactatagg ctgaatgtag ctcctaataa gaaaaataat
     9121 gcaacagaat ttagaaaaaa actgatcagg agattaatat tctacaatga agtattgatc
     9181 gtttctccgt ctaataattc tagcgaaata tttattgcgg atagttggga tgtcacagaa
     9241 tatgcattga aagatgatgt gttttctcaa gtgcaaatta acaacatagt ccttgataga
     9301 gaatttctag aaagtgatgt tatctatata aaatacgcag atcaacaaat taggcaacta
     9361 gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag
     9421 cgctctaacg ctcgtagata cgtactgaaa gggaatttat tccgaccgca agacaataca
     9481 acacaagatc aaatcaataa aatgatgaca tcacaattta aggcttttat ggaagctgat
     9541 aatgcaggtg cggtatttca attacaaaat gagtacacat tagaagattt cagcggaaac
     9601 ttccaaagca attcaagaga tataaaaaac ttaatagacg acatctttga gatgacagca
     9661 gcagcgtttc acgttccgaa aaacctacta aagggagaca tgagtgggtt atcggatcaa
     9721 gtggacgctt ttttaatgtt cgaaatcata ccgattgctg aacttattca ggatgcgttt
     9781 aacgctagtc tctatgaagt agaagaatac ttgtcaggga attttgtacg tgtggataca
     9841 actatgatca agattactag cttcaaagat ttggttgacg ctattgatgt aggcattaga
     9901 aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg ctctgataag
     9961 gcgatggcag atgaaatatt tataactaaa aacaatcaac aagtatcgaa aggaggtgag
    10021 gcgaatgacg acaatgaaaa catttctagc agtaaagaat gaaggcgcag taccgcaaat
    10081 ttttattcag ggatttattg gttctagttg gttctttgaa gggaatactg acaagggaat
    10141 caaaaatatt ttggatagtc taggtgatca agaagaaatt gaagtagtaa ttaattcaaa
    10201 cggtggagac gtatttcaag ggattgctat tgggaactta cttaagtcaa ataaagcaaa
    10261 agttaacgtt gtgattaacg gcttagccgc tagtgctgct tcaattatcg caatggctgg
    10321 cgatactata aaaatctaca acaatgcaca attgatgatt caccgcgctt ccacatacgg
    10381 agaaggaaat gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc
    10441 ggtaaaggct tcatataaaa cacgattcaa tggcacagat gaagcattgc aagaacttct
    10501 tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt tatggattgg tcgatgaaat
    10561 tatcgatgca gaaaatagct caggtactga agctaaaaaa gaacaaagcg ttgaagaaat
    10621 tttgaatgac gttaaagaaa aaagagcaga aaaaattgct gcatttacag cagcattaaa
    10681 taaaacattt ggacaaggag atgtaaaata atgacagtta aaaatttaaa aggtgtaaca
    10741 gctgcaagcg accaattgat gaaagctttt aaagatggta acgaagaatc ttttagcgca
    10801 gctatggtaa gcttatctaa ggaaattcag gataaaattt tagaagaagc aacagcaaaa
    10861 aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg tattaactac acaagaaaca
    10921 aaattctata acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca
    10981 gctactgtat ttgaacgtgt atttgaagat ttagaacaat ctcatccact attgcaaaaa
    11041 attacttttg ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca
    11101 gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt tagataatgg ctttgatgta
    11161 attaacatga agcagttcaa gctatcaggt tatattcctg tatgtaaggc aatgcttgat
    11221 ttaggtccag tatggttaga tcgttatgtc cgtactgttt tagtagaatc attgagaatt
    11281 gcattagaac aagcaattgt tgatggtact ggtaaagata tgccagtcgg aatgatgcgt
    11341 gacatgagca aacaaactag cggagaatat gctgaaaaaa aagcagaacc tattacagct
    11401 ttagatgctg taactatggg cggtttgatg gcgcgactat caaaattcaa tatcgaaggt
    11461 gtgaatgatc cgatttatcg taatgtgaat ccttctgatg tggtcctaat tgtgaatcca
    11521 actgattact ggtctaaagt attcccagct aagactgtac taactgctaa tggagaatac
    11581 gtacaagtat tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa
    11641 gcagttattg gggtagcctc agattacttc atgggtgtag gatctacact aaaaattgaa
    11701 gcttcagatg aataccattt tgttgaagac gaacgcattt atctagctaa acaatatgca
    11761 aacggtcaac ctaaacgtaa cgatagtttc attgtattag atattagcgc tttgggaact
    11821 actactacaa ctacaaaacc aacaaccaca acaactacaa cacaagcgta ggtgatcaga
    11881 atgaagtata ttctttgtca gccggcaatc aatcggttta aatgggagct tgaagtttgt
    11941 ttaactaatc tgaagaaact aggaatcaaa gatatcgtat tgcttttcag cagacacgat
    12001 gatcagattc ctattttttt tgagaaggaa tatggtgttg aagttcatgt gtacgatgat
    12061 ctgcgggacg acaaagagta tattccttcg attaaaccat atttatggtg gaaatattta
    12121 gaagaagatc attcgcgtga ggacgaccga tatttctata tcgattcgga tgtcattttc
    12181 aataaaagaa ttaatttgcg caaattgcct tctaaagatg atgtttggta ttgtagcgac
    12241 tgctgtagtt atctaagtct tgattatatt agaagctgtg aaaacggaga aaatattcta
    12301 aaagatatgg caaacattgt aaatgttaca gtagaatctt tggaaactat aaacactaat
    12361 tcaggaggcg cacagtgggt tattaaccgt cctaaagcga attattggaa aaaggtttat
    12421 ctggattcta atcggctata tcgctacctt agagggcaaa aaacaaatat acaaatctgg
    12481 acagccgaga tgtgggcaca gctttggaac atgatgtatt tcaatattgg tcctaaagtt
    12541 cacgaggaat tagacttttg ttttgctact gatccaatag aaaaagttaa agaagtaaaa
    12601 atcttacaca atgctggagt aacaacaaac gatgaagatt tatttttcaa agggagatac
    12661 gtgacttcta cgccttttga tgaagattta tcatttgtaa acaagaaaaa atgctcttac
    12721 gcatatgtta aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg actgaagaat
    12781 tgctaatagc tgtgaaggat aatatttacg ttacctggaa cgaagaagat gagtcaatta
    12841 aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca acactttctt
    12901 tttctcctga aagcttagaa tacggattgc taatcgaaag atgtagatac gactggaatc
    12961 gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt attaggtttc attcaacatt
    13021 atgcgctaca agaatatatt gcaggtgatg tgaatggcga ataatcgtag actcgaagaa
    13081 acattcaacg atggttggtt aaagattttg acgcaaacta ccaaaagaaa tgaactagga
    13141 aaaaagattg gtgtagaaga tacagaaatc acttctttaa aatttagaaa tctttccatg
    13201 agagatagtg atataacagc tatggatgcg atgggatcga aattaactaa gaaagtaaag
    13261 actccatttc atccaatcgc caagaaattt aataaagatc aatattttat cgtaatcgat
    13321 agtatgcgtt acaacgttat ctatgccgat tacgataatt tttatatcta tttttatctt
    13381 gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt taaataagca
    13441 aatttctgct atcaaaactt ccttagaaga gcattttggc ctcaaactct ttcaagactc
    13501 cgtaggcgag gatgagctac ctgatgattt taattacttc attctcgaaa caggagaaat
    13561 agaaatgatc actgagccaa aatatagcgt gggtcaaaat ctatatctaa ctttctattc
    13621 agaaaataga gaagatttaa caggagactc actagatatt atttcattga ttcaaaatcg
    13681 ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa ctagagaacc aagatcgcta
    13741 tatcgatcaa ttggtattta cgtttagacg attattgaag agtgattgtc atggctaaaa
    13801 atagttggga gctaaaaata aatggacatg atgaacttct tgtgcggatg gaacgctatt
    13861 caagcgagag cgaacgactg attaacgaag cattgaaatc aaaaggttcg gctattgcag
    13921 tggataggat tacagaaaaa attcctgttt ctgaagcaga tttaagaaga gggcaccaac
    13981 acgcaaaaaa tagtcgtcca cttaagactc aatacattaa tttgggtttc atcattagac
    14041 ctacaagaaa atttgagtat ttaaaatatc ctgatttggg gataggtact tctaaaagaa
    14101 atcagccgga cgaatttatg agaagaggat taggtcttgc acttgatcca attacagaac
    14161 ttctgattcg tcaattcgat aaattaaata aataggggga acaacaatgg ctaaaacaaa
    14221 aactgtagta acaacgttcg ataacgtaag tatcaaacga attgctttta attttaagaa
    14281 cgcaggaaat gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa cagaaatgca
    14341 aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac caatcaatat
    14401 gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgtttca atgggttgaa
    14461 acaagatgga cgtattaaac caggcattta ctcttacggt cctgattccg taggcgaaga
    14521 tttctcactt gctgcagaga tcgtggatga cttcgaagaa aatagaaagt tagttggtat
    14581 gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt
    14641 agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga
    14701 agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc
    14761 tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa
    14821 acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt
    14881 tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga gattttaaca
    14941 cagacgagtt tgcaaaaatg actgtgggaa aaggatccat ggatatttct gttattcaag
    15001 gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat gactgattat atttcattcg
    15061 atgaatttat cgataaatgg gattttgata tggctactgc cagctatatt tatcaattga
    15121 tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa
    15181 aaaagcttca aaagtaaaaa ttccaaagct cttagttgaa acgtgggtcg atgtctattc
    15241 gatgttgacc gacgtttttt ctatgccttc agatttggtt ttaagcgata tctgtttaga
    15301 tgacattttg caaatggctt acaacaagag tgcttatgaa ggatggaaaa actatgcaat
    15361 aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag
    15421 cagaagtaac tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg
    15481 ctgaattaac taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg
    15541 gttctgaatc agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac
    15601 aaagtcaaaa agttgaagta actagccaac gtttagccaa tgcgaaaaaa tattatggag
    15661 aaaattccac cgaagttcag aaacttgaaa gagaactgat taatcaacaa acagcgcaac
    15721 aacgtttgtc aaacgaaatt gataaaacga gtaatgcact agctcaagca aaaggcgaaa
    15781 tacagacgta cgagtctaca atgcaacagt tggatagcga acaaaaaaat gttcaagcta
    15841 gtgcttctct gattgaatcc gaatacaaaa aatggcaagc aactgctggt caatcagctt
    15901 ctgaagccga gaaattagcg aaagcccaag aatatgtttc tcaacaatct gaaaatgcgg
    15961 agaaaacgat agatatccta agacgacagt tagaagctac acagtctgaa tttggcgcta
    16021 catccacaga agcaatgcag atggaagcga agcttaatga tgctgaacgt gaatttgaag
    16081 agttaggaca agctgctaaa aatgtagata caactaactt ggacgatatc ggaagcaaaa
    16141 tagatatgaa taatttaatg gaagcttctg acgttttaag cgacattggc gataagctta
    16201 cagaattagg gaaacaagca gtggactctg ctaacagtgt aggtagttcc cagagtaaga
    16261 tacaagctaa ttttggtttg tctaaacaag aggctgaaga attaacgaat gtagccagag
    16321 acatttatta taaaggtttt ggagaatcgt tagatcagtc cacagatgca ttgattttgg
    16381 taaagcgtaa tttaggagat ttaaataatc aagatttaca aaatatcacg gaacaagcta
    16441 tggtcctaga aaacaccatg ggcgctgata tggatgaaac gttacgtggt gtaaatggct
    16501 taatggtcaa tttcggcttg agtgctcaag atgcaatgga tttaatggtt tcgggtactc
    16561 aaaacggttt agataaaacg cacgaattag gcgacaatat ggcagaatat agccaattat
    16621 ggagtcaaat gggatattca gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg
    16681 cgggtgctta taaccttgat aaagtcaatg acttagttaa ggaaatggga atatcgttaa
    16741 cagatggtcg atttgagcaa aacatggata tgtttagtga aagtactaga aaagcttttg
    16801 aagagtggaa aaatggcgga ggaacacaaa aagacgttat taattccatg attcaagatt
    16861 ttagcaatat ggatggtcaa tacgaccaat taaataaagc ttcgacaatt tggtctgcgc
    16921 ttggcgaaga taatgcgatg aaagttgtcc aatctttaac tgatgttaac catacatttg
    16981 atgatgttag tggatctgca caaaaaatga atgaagattc tactactccg ttgcaagagt
    17041 tgaacgggaa aatagctgaa ttaaaggatt cattagctcc tataggcaac acaatcatag
    17101 atgcactcga accagtaatt gattttctag gaaagatggc tgatgcgttt aataatcttc
    17161 cacaaccagt acaggatttc attgttgcta taggtggtct tacagcagca tttggcatac
    17221 tgttaccggc aatactagcc gtgtcatatt tatttggtcc gatgatgctt ataattggag
    17281 gaattatagc tgttatagca ggcgttattg tggcaattaa gaactggggt gcaattactg
    17341 actggtttag tggtttatgg aaaaaattta ctgattggtt gggtgatact tgggaaagta
    17401 taaaagacgg agcctcatca gtttgggatg gggttaaaga aacctggtct ggatttgtag
    17461 attgggttca agatatttgg caaggagttt ctgattggtt tggagagcta tggagcggat
    17521 tagttgaagg agcttccaac atctggcaag gagtccaaga aacttggcaa acattcgttg
    17581 attgggtttc aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca
    17641 ttgtaggaat tgttcaaata ccatggacat taataacgtc attgattcaa gctggtatta
    17701 atattatcgt gggtattttt gatgtagctg gacagttatt gggcgcagct tggcaagctg
    17761 tttggacacc tatttctgat ttccttaaaa atacttggga tactatgaca caatggataa
    17821 gtatcgcttg gaatgggatt gtaactacat tccatactat atttgatcca gtagtggcat
    17881 ggtggaatgg tatatggaca gctattagta ctacggcttc aaatatttgg aattcaatta
    17941 gtgcaacagc ttctagtatt tggaacagta tcaagaatac aatcactagc ttggtacaag
    18001 cagctgctac agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt
    18061 ggaattcaat cagctctaca gcatcaaata tctggaatag tgtgactagt agtataagca
    18121 atgctataaa cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga
    18181 ttagcggaat ttggaacggt atcaaaaata ctgctttgaa tctttggaat ggaattacaa
    18241 gcactattag ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc
    18301 taacaggtat tgtttccgac atattcaata atgttaaaag tacaattgct aacatatggg
    18361 aaggcatcaa aaagactgtt agcgctccaa ttgattggat tagagacaaa atcagtggca
    18421 tttttgataa tttgaatatt tctataccac atattccgtt accggaattt atcatggaag
    18481 gcagctttaa cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag
    18541 gaggtatctt tactaaacca actttactag gtggaatgaa cggtgtcggt gaagcaggac
    18601 ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat cgtatcttga
    18661 gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac tacgaattca
    18721 atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact caacaaatca
    18781 ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa acaggagagt
    18841 atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt gtatacattc
    18901 tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat
    18961 accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag gtggcttagt
    19021 acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt tagaggatgt
    19081 ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaactatgc
    19141 aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga ctatcgatat
    19201 tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata ttactgcgaa
    19261 accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa
    19321 cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac
    19381 ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag tagcaggaac
    19441 tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg acggagatat
    19501 tttagatcgg tccaacaaaa tgaagaccat gcaatatccg acattgcaag tggatatcaa
    19561 tcattttaat tgcactggtt tgagcaaaat acaaatttat cgtaacgggt taaggtaggt
    19621 gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga ataatttagc
    19681 tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa cagctactgt
    19741 tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa acggaactaa
    19801 tgcagatttg attgtagacg gacgtattat tcagtgttat gtagatgaaa atcgagcaaa
    19861 acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa
    19921 agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg acagcggaac
    19981 agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga aaccagctat
    20041 cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa
    20101 ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg
    20161 tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta cggatcataa
    20221 aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg atgctcaaag
    20281 tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag acgagatcac
    20341 tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg gatatgtttc
    20401 ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag ctgctaaaga
    20461 ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga ttgaattagt
    20521 accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag tttcgatggg
    20581 tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga gaattgtgga
    20641 atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa acgtcaaaac
    20701 aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg ccattgatca
    20761 attgaaaaac ggtggcgaaa tcagtgattt actcaatgat attgtagatc atcaaactga
    20821 tatgattact ggtcaaaatg gtggttatgt tttattagat cctaaagaag cgcctagtcg
    20881 tattttgatt atggacacac cagataagaa taccgcaagg aatgttttac aaatcaacaa
    20941 tgctggtatt ggtttttcta aaactggcat taatggaaca tatgaaacgg catggacgtt
    21001 agatggcgga ttcaatgcct cgtttattac ggctggtgaa atagtaggaa ttactattag
    21061 aggtactaca ttaattagtg atggtgctga ttatagaaca agtattgcta atggcaaaat
    21121 gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac gtgattatgt
    21181 aagtgctgat gccggtattg tatcatacac catgaaaact ggtggtggtt tcatgattag
    21241 aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca gaccgtttct
    21301 atcttttggt gcgcccaatt tcaggtatag caatgctagt tatgtaactt ctggcgacgg
    21361 tagttcttta agcattaatg gtagtgcggg taactcatgg gaatttaagg tagctggtag
    21421 gactatgaaa tttactagtg atggtatgct aacgttacca ggttgttttt ttggttcatg
    21481 ggaagatggg aaacttgcta ggtttgaaca atcaacggta caagtatata aagattttac
    21541 tgttagaggt actaaaaact caactgtacc aacagaacat tatggacaac gactattgaa
    21601 cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta caggtgacaa
    21661 tggtaaagtt cgtgttgata ttgaccccat gtttgctgaa acagtaaatc taagtcggta
    21721 tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg aagatattga
    21781 ccatttcatc attgaaacta gtaagccaaa cgtattagtt agatggaatt tagtggcaca
    21841 ccgtctaggg tatgaagata ttagattaaa agaggataca gcatatgata gcacagtgct
    21901 tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag cagtttatat
    21961 aatttggctt tagatttcag caaagaatta aactacacca aagctattat ggctcgtcaa
    22021 ggtgataaag ggattacggt gacggttaaa ccgtttctaa atggcttgca gatggatacg
    22081 agtggcggaa catttacttt aaaaggaaca acaccatcta accgttacgt agataatgtt
    22141 gcaactagtg taactagtga agaagtcacg ttttctcttg atggcacatt tatgagtgaa
    22201 gcaggatatt ataaacactg ctacgtagaa tatagaaaag acaatcaaat tttaacaacg
    22261 caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca agccgatgaa
    22321 tatgtttcgc aattagaaga gttgattcga aagtacaacg aaacttttga tgcttttatg
    22381 gctgaaatca aaggtagagt ggatagctta aatcaacaga ttactgattt aactggtcaa
    22441 gctaaaacgc tacaagacaa gttagatgct ctgaaagaag aaatttctaa gttaggtaac
    22501 ttacaagtga tgtacagtaa cagcatcgac ttcggggact atgattattc ggagaacccg
    22561 aatttaatgc catatattac agaaccttgg gttggacctt tgttaggtaa tggacataca
    22621 gttaaagata gtgttaaaag ggttataact catactaaaa ctagaacagc taattccggc
    22681 gacatattat cgcttggctt aggtattccg tgtacagcgg aagctaataa taggtatctt
    22741 ataaccactt tacgaccatc tacgacatac acattaagcg ttactatgag tgtcggttca
    22801 gattggactg gagagaccaa tactataggg gttaggctta gatatttaaa tgaacaaggt
    22861 ggtatagaac tgcccattaa tgcattaata ccagcaaatg tagaacgcga taaaatggtc
    22921 actcatacat ttactggtat aacaaaagat aatgtaacta gcataacaaa ctgttatgtt
    22981 gaaatattct cacttaatag tgaatataaa ggaacggtta gtgtcagcta tgacgttaag
    23041 ttaaaagcac actaccctaa cttactagat ggcccttact ggttaggtaa agttccttta
    23101 ggcgagaata ttgctgaccc tacagttgtc tttccacaca aaactagtga atatatggtt
    23161 tatggtagac gcaatacaga aaactatatc gctgaccaaa catataccat atcaatgaaa
    23221 gcaactaaac taacagttca atcgtttgcc gtgtatattg ctgctggccg cgttaaagta
    23281 ggagacatga agcctactga ggggttagct aatacatggg agttaacttt tactgtaact
    23341 aagcaacata tagatagtgg ggtaactaat tatctggaaa tataccaata cccgtcagct
    23401 acaaaaggag ctgttcaaat tgagtggctc aaactagaaa aaggcaatac tcgaaccccg
    23461 aatattagtg agtataaata tcgtggtact ggtatgcgtg attcaaacaa tccaaaagat
    23521 tatgtttggg atctagcacc agaatatgtc gaagataatt tggccacaga tattaaaatt
    23581 tctgaaatta ctggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg
    23641 cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg
    23701 aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat
    23761 acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
    23821 gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagcag tggtggaaat
    23881 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
    23941 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
    24001 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
    24061 acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
    24121 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
    24181 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
    24241 atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt
    24301 gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg
    24361 ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac
    24421 gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag
    24481 aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag
    24541 gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg
    24601 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
    24661 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
    24721 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaaaaagc
    24781 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
    24841 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
    24901 ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta
    24961 ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga
    25021 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
    25081 cacatgcaga aaaattggta gaacacacgc aacaaattaa aactttattt agggagagaa
    25141 gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc
    25201 actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg
    25261 tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg
    25321 aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt
    25381 actattactg cgatgagcct gttaacggct ctttttttat tacccattaa tacgtttgct
    25441 tatactatta atgacgagta taatttagcg ccgaatcaag gagactccag attagcaatt
    25501 cctaacaaaa ttattttgca cgaaactgga atagatgcac cagcaagaaa cgtagccgcc
    25561 aacatgaaaa ataattataa cggaagcaat tcttatacta cagatgttat tggtgacggt
    25621 gggattgttt accgtgtggg tgagcaagga tatgtttcgt ggggagctgg taacgctaat
    25681 ccttatgcgc ctgtacagat tgaattacag cgcacatatg ataaagcatt gtttgaaaaa
    25741 aactatcgag cttatattga atatacaaga gatagtgcaa aaaaatatgg aattccattg
    25801 actcttgatc aaggaacttc tttatttaca aaaggaatca tttctcattt gtgggtgaca
    25861 aattatgttt gggggaacca cacagatcca tatggttact tatcgcaaat gggagttagc
    25921 aaagaaaagc ttgcttatga tttagctcat ggatttaccg atgaaaatcc aacaacttct
    25981 gaaaacaagc ctgtcattga tccaacacga gctggtgcag ctaatcctac gctgacagat
    26041 ggaacaaatc acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg
    26101 gctggatggc acattgctaa ctataaatac gagtatattt tcattatgga ctacaatact
    26161 gggaaagaat tagctcgagt aagagctgat ggaatttata gaccagatgt aaaccaagct
    26221 tataatactt ctggaaatgt tggttatcat gtatctttca atatgcgtaa ttttcctagt
    26281 aagaaagtct atgtaatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt
    26341 gcgcaagatt tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc
    26401 ctcgttgagg agcagtacat aactatattg acaactataa aaattattcg ataaaatagt
    26461 gatgttatcg catatcttca ctatcaccca t
//
LOCUS       DAYYHB010000034        26078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000034 DAYYHB010000000
VERSION     DAYYHB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.035"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..422)
                     /locus_tag="ACHA6B_001370"
     CDS             complement(9..422)
                     /locus_tag="ACHA6B_001370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8948954.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            501..932
                     /locus_tag="ACHA6B_001371"
     CDS             501..932
                     /locus_tag="ACHA6B_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8948955.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(1031..1264)
                     /locus_tag="ACHA6B_001372"
     CDS             complement(1031..1264)
                     /locus_tag="ACHA6B_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8948956.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            1447..3117
                     /locus_tag="ACHA6B_001373"
     CDS             1447..3117
                     /locus_tag="ACHA6B_001373"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8948957.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            3264..3650
                     /locus_tag="ACHA6B_001374"
     CDS             3264..3650
                     /locus_tag="ACHA6B_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948958.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            3637..4335
                     /locus_tag="ACHA6B_001375"
     CDS             3637..4335
                     /locus_tag="ACHA6B_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8948959.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            4340..4804
                     /gene="lspA"
                     /locus_tag="ACHA6B_001376"
     CDS             4340..4804
                     /gene="lspA"
                     /locus_tag="ACHA6B_001376"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8948960.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            4801..5712
                     /locus_tag="ACHA6B_001377"
     CDS             4801..5712
                     /locus_tag="ACHA6B_001377"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8948961.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            5947..6486
                     /gene="pyrR"
                     /locus_tag="ACHA6B_001378"
     CDS             5947..6486
                     /gene="pyrR"
                     /locus_tag="ACHA6B_001378"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8948962.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            6500..7783
                     /locus_tag="ACHA6B_001379"
     CDS             6500..7783
                     /locus_tag="ACHA6B_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8948963.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            7796..8722
                     /locus_tag="ACHA6B_001380"
     CDS             7796..8722
                     /locus_tag="ACHA6B_001380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8948964.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            8741..10021
                     /locus_tag="ACHA6B_001381"
     CDS             8741..10021
                     /locus_tag="ACHA6B_001381"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8948965.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            10026..11102
                     /locus_tag="ACHA6B_001382"
     CDS             10026..11102
                     /locus_tag="ACHA6B_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8948966.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            11107..14292
                     /gene="carB"
                     /locus_tag="ACHA6B_001383"
     CDS             11107..14292
                     /gene="carB"
                     /locus_tag="ACHA6B_001383"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8948967.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            14346..15122
                     /locus_tag="ACHA6B_001384"
     CDS             14346..15122
                     /locus_tag="ACHA6B_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8948968.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            15119..16051
                     /locus_tag="ACHA6B_001385"
     CDS             15119..16051
                     /locus_tag="ACHA6B_001385"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8948969.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            16048..16764
                     /gene="pyrF"
                     /locus_tag="ACHA6B_001386"
     CDS             16048..16764
                     /gene="pyrF"
                     /locus_tag="ACHA6B_001386"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8948970.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            16771..17400
                     /gene="pyrE"
                     /locus_tag="ACHA6B_001387"
     CDS             16771..17400
                     /gene="pyrE"
                     /locus_tag="ACHA6B_001387"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8948971.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            17388..17702
                     /locus_tag="ACHA6B_001388"
     CDS             17388..17702
                     /locus_tag="ACHA6B_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948972.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            17740..18381
                     /locus_tag="ACHA6B_001389"
     CDS             17740..18381
                     /locus_tag="ACHA6B_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8948973.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            18424..19116
                     /locus_tag="ACHA6B_001390"
     CDS             18424..19116
                     /locus_tag="ACHA6B_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8948974.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            19113..20015
                     /locus_tag="ACHA6B_001391"
     CDS             19113..20015
                     /locus_tag="ACHA6B_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8948975.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(20173..21879)
                     /gene="efbA"
                     /locus_tag="ACHA6B_001392"
     CDS             complement(20173..21879)
                     /gene="efbA"
                     /locus_tag="ACHA6B_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8948976.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            22069..22692
                     /locus_tag="ACHA6B_001393"
     CDS             22069..22692
                     /locus_tag="ACHA6B_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948977.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            23142..24191
                     /gene="trpX"
                     /locus_tag="ACHA6B_001394"
     CDS             23142..24191
                     /gene="trpX"
                     /locus_tag="ACHA6B_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8948978.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            24188..25069
                     /locus_tag="ACHA6B_001395"
     CDS             24188..25069
                     /locus_tag="ACHA6B_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8948979.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            25072..25842
                     /locus_tag="ACHA6B_001396"
     CDS             25072..25842
                     /locus_tag="ACHA6B_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8948980.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
BASE COUNT         8340 a         4589 c         5785 g         7364 t
ORIGIN      
        1 tttttaattt aaagagtctt tttttctttt aattgtttat ggagagcctg acgtgcaaga
       61 ttatctgctc ctttattttt gttttcgggc aaccattgca ggatcaataa agaaaattcg
      121 gatagaagtt cttggattct ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt
      181 ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt
      241 aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg
      301 tttgtcccta tagaaagcgg gatagatagc tgttcttgtt taccttgata gacaatcaag
      361 attccacctc cactaggacc agggttgcct tttgtagagg catcaatgta tacttttaac
      421 ataaaaagag attctccttt ttgttcaaac taatgttatg gtaagattat atcattactg
      481 aatcggtgag aggttagaga atgaaaaaaa atattcgatg gcagcctgaa ctggcacata
      541 cgatcatcta ttggtcattg accttgatca ttctgttttt tagtctgatt ctttctttag
      601 aaaacacagg tccttattgg aaaagcaatt ttgttttagg tatttttctt gtttttctat
      661 ttctaggttt gcggcgttct tttattttaa aatcaaactc tgtgattata cgttatgctg
      721 ctttttggaa agaccgagaa atatctgtcg gacagattcg atctttgacg ccaactaata
      781 ctggggtgca aatgatgttg caagatgaga aagccccatc tgttttcatg atgcgaaaaa
      841 aagatcaagt agtccttttt gatcattttg aaaaaaacgg acccaaatca gaatcaattc
      901 cttatcctta tacgaaaaaa agaaaaagct aattttaaaa atcagaacct aaaaaaacga
      961 atttttacag gaaaagaaaa gagtgagacg tacggttcat cccgtttagc gtctcactct
     1021 atttgtttat ctattcatgc atactagtat cggtagtctg aacgttgcct ttcttgacat
     1081 gggcagcttg tgttcctcga ttcccttcga tgatctcaaa ttcgaccact tgattttctt
     1141 ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt
     1201 catcataact gataaagccg tatccttttt tgttgtcaaa ccattttaca attcctgtgg
     1261 tcaaagcact cacgaccttt ctaaagtctg atcatagcct tcattataaa gagtatttat
     1321 aagtacgtca aataaattaa aaacctgtaa ttttatgttt tttttctgaa taaaaaaagg
     1381 tataattggc atatcttaac aaagaagata ttttgttgaa taaagttcgt gaaaaggggt
     1441 aatcaaatga agaatgatct gcaaatttca caagaaacag agctagaacc gattgttgaa
     1501 gtggctagta aattagatct taaggaagac gatttggaac tgtatgggaa atataaggcg
     1561 aaaatcaatt tttctggaat caatcgatca aaggataacg ctgaggggca tttgatctta
     1621 gttacttcca tcaatccaac accagctggc gaaggaaagt caacgatcac tgtagggtta
     1681 ggagacgcgc tcaataaaat caataaaaaa tctgttattg cattgcgaga accctcactt
     1741 ggtcctgtga tgggaatcaa aggtggtgct gcaggcggag gatatgcaca ggtattgcct
     1801 atggaagata tcaacttgca ttttactggt gacatgcatg ctatcacaac agctaataat
     1861 gcattatctg ctcttttaga taatcatatt catcaaggaa atgaattgaa catcgatgcc
     1921 cgccgagtga tttggaaacg ggtagtcgat ctcaatgatc gtgaactgcg taaagtaatt
     1981 gttggcttgg gtgggccaat ccaaggtgtt ccaagagaag atgggtttga tatcacagtt
     2041 gccagcgaaa tcatggctat cttgtgtctt gcggcagacc ttgaagattt aaaggctcgt
     2101 cttgcaagga tcgttgtcgg ctatacattt gaccgtcagc ctgtgactgc tggagacttg
     2161 aaagcagaag gcgctctggc attactgctg aaagatgcaa tcaagccgaa tcttgtacaa
     2221 acaatttacg gaacaccagc ttttgtccac gggggaccgt ttgccaatat cgctcatggc
     2281 tgcaatagta ttttagctac caagacagca ttgagattag cagattacgt tgtcacagag
     2341 gcaggatttg gtgcagatct tggcggagag aaatttttag atatcaaagt acctaacttg
     2401 aaaaaagcgc cggatgctgt ggtgattgtc gccactgtcc gtgcattgaa aatgcatggc
     2461 ggaatgaaaa aagatgaact taaaaatgag aatcttgatg ctttgaaagt aggttttgca
     2521 aatttaaagc gccatatacg taatatggaa caatatcagc tacctgtgat cgtcgcaatc
     2581 aatgaattcg taacagatac agacaatgaa ctcacactgt tggagcactt gtgtgaaaat
     2641 caggggattc ttgccaagag agcgagtgta tgggcagatg gagcagaagg cggcgttgat
     2701 ttagcaaaag ctgtagtacg attgattgat agaaaagaag ctaattacaa gccattatac
     2761 cgcctagaag aaacgataca agaaaagaca gaaacaatcg tcaagaaaat ttacggagga
     2821 aatggtgttg ttttcagcaa aaaagcgcaa aaacaaatag aagagtttac gaagaatgga
     2881 tgggacaaac tgccgatttg tatggcaaaa acacagtatt cattttctga tgatccttca
     2941 ctgctaggtg caccggaaga tttcaccatc acgatccgcg aaatcatccc aaaattaggg
     3001 gcaggatttt tagtagcctt gactggagat gttatgacga tgcctggttt gcctaaaaaa
     3061 cctgctgcat tgaatatgga cgtgacaaat gacggagaag tgttgggatt gttttaagct
     3121 tgattataat ctaccatcga aggagctgta ttttgactac agcttctttt tttagacgat
     3181 agggaattaa ttttgtttac actaggatca ttctttttct ttattgtatt ttgagctata
     3241 ctaaagcata ataggagggt atgatgaaaa cgacatttat aagaaaaacc ggtttttacg
     3301 gtatgggtag ctcgattcag ctaagaatca acaatgaaaa acgcagtgtt ctcagtcatg
     3361 aacaaaagat aacgcttgat ctggaagtgc cctttacgat gcaggtcacc ttttattggc
     3421 taaaaagccc cgtttataca gtgaaagaag caaagcctgc ttatgtgatc acaatgaatt
     3481 acttgattct tcaaatctat ccgtttttat ttctagcaac tgggttgggg gccgttttcc
     3541 tacaaaattt atttttcagt ttttttgcag ttattgtgtt gattgcgttc tttttctata
     3601 taaaaaaccg ggcatatttg atcaaggagg aaaattatgg caaactctga tcttttttta
     3661 atcagtttca atcgaatcga aaaatggatg agagacgaga tgggaaatgc acgaaatatg
     3721 ggatttaccg aacttgtcag acgcctagct caaagaaaac aattaatgat cagaaaatac
     3781 gaagatgatc ttttacagct tgctcagtta aggaatgcga tcgtccatga tcggatcgca
     3841 gtcgattttg tcattgcaga gccaaatgat tggacagtca agcgaatcca gctgatcgaa
     3901 caagaactga ttcagcctga aaccgtgctg cctcgatttg caaaacgtgt aacaggtttt
     3961 gaacaagatc ttccgttact tgaactgtta aagatagtag cagaaaaaag gtattcacaa
     4021 tttccgttat acaataaagg aaaatttgta gcattgatta ctttacgtaa tatcgggttt
     4081 tggctagcaa aagaaagcca aaaaggtccg gttgatttaa caaataaacg agcgttggat
     4141 ttgatcatcc aaaacggaaa gtacacaaat tatcattttg ttccagctaa aacacatatt
     4201 tacgaggttg aagcgatgtt tcgtgagcaa gggactttgg aagccgtact tatcactaaa
     4261 gatgggaatc ccgatggtaa tctgctcggg atcgtacgac ctcgtgatat ttataaacaa
     4321 atagaaaagg attgattttt tgttaattat ttattggata ttcagcgctt tgctagtagg
     4381 agtggatcag ttggtcaaat ggtggatcac tgataatcta gcgcttggag agacgaaaaa
     4441 tcttatacca ggtattttgt cactgaatca tattcgaaac actggagccg catggagtat
     4501 gctagaaggg aagatgtggt tctttactat agtgacactc attgcagtcg ttgtgattct
     4561 cacattgatg attaaaaatc gtgaaaaagg taatcgctgg ttcatgattg gattgagttt
     4621 gattctagct ggtgcgattg gcaattttat cgaccgtatt cgtcttggct atgtagtaga
     4681 tatgttccag acagatttta tgagctttcc gatttttaac gtagcagatg tgacacttgt
     4741 atgtggagtg atttgtatgt taatttatat tattttagat gaaaaggatc aaagaaaaaa
     4801 atgacagaac ttaaaacgac gattcaccat gaatctggta ggatcgataa agtattgact
     4861 aatttattca ccgattacag tcgttcgcaa attcagctgt ggctgaaaaa tggagcggtt
     4921 tctgtcaatg gacagccagt caaagcaaat tacaaagtaa aacaaggaga cgagattact
     4981 gtcttgatcc ccgaaccaga aacattagat gtggtcccag aaaatatccc gttgacgatc
     5041 gtttacgaag atgaagatgt agcggtgata aacaaacctc aaggaatggt cgttcatcct
     5101 tcagcaggtc atacgcatgg cacaatggtc aatgcgctga tgtaccatat caaggatctt
     5161 tctacgatca acggcgttat tcgtccaggt attgttcacc gaatcgacaa agatacttcc
     5221 ggactgctaa tggttgccaa aaatgaccag gcacacgaat cattagctca gcaattaaaa
     5281 gataagacct ctttgcgcaa atatgttgct ttagtccatg gagagattcc tcacgaaaaa
     5341 ggaaccatca atgcgccaat cggccgttcg aaggaaaacc ggaaaatgca ggcgataaga
     5401 gaagatggga agtcggctgt cacacatttt actgtattgg aacgtttcga aggatttact
     5461 ctggttgaat taaaactaga aaccggaaga acacaccaga tccgtgtgca tatgaagtat
     5521 atcggttatc cgttagctgg agatccagta tacggaccga agaaaacttt gaaaggcaat
     5581 ggtcagtttc tccatgcgca aatgttaggt tttacgcacc cgactacaaa cgaaaagatg
     5641 atctttgaag cacctcttcc agaagttttt gaaaaaactt tagaaaaatt aagaaaaaac
     5701 attgcatttt aatttagata acggtatagt aattaacaac gaaagcataa aacttaatag
     5761 aattccttta agtttagtcc cgtgaggcta agaagggtgg gagcaaggat acgtatggtg
     5821 cgccataagt agattgctca ttgacatagt atagctatca gtgtatcagt atacgctcag
     5881 tctaaacatt cctcctgtcc tcttagggca ggagtttttt tatgcaaaaa agtaggggga
     5941 atgaaaatgc aagcaaaaga agtagtagat caggtaacca tgaagcgagc attgactcga
     6001 attacttatg agattatcga gagaaaccat agtatccaag atatcgtatt ggtaggtatc
     6061 aaaacacgag gaatctacat tgcatcaaga attgccgaac gcctgaagca gctagaagat
     6121 atcgatattc ctgtaggaga actggatatc actttatacc gagatgacaa aaaagaaaat
     6181 ccagaagaac cggaacttca ttcttctgac atcccagttt ccttagaagg aaaagaagtt
     6241 attttgattg atgatgtttt gtatacagga cgaacgattc gagcagcaat ggacgcagta
     6301 atggattttg gcagaccaag aaaaatttca ttagcagtcc tggtcgatcg tgggcatcga
     6361 gaattgccaa tccgtgcaga ttatgttggg aagaacattc caacatctcg ggcggaagag
     6421 attttagtag agatgcaaga gcttgatgga caagatcgca tcatgatttt gaaagaggaa
     6481 gattagagga gagagaaaca tgtcggagaa aaaaggattt cataatgatg acgtggtgtt
     6541 agatatccac gatcgcccaa ccccaggaca ttgggtagga ttaagtttac aacacttgtt
     6601 cacgatgttc ggagcaaccg tgttagtccc catcttagta ggtatcgatc caggtattgc
     6661 gctagtcagt tcaggactag gtacattggt gtacttattt actactaaag gaaagatacc
     6721 tgcgtactta ggaagcagct tcgctttcat tgcagcgatg cagcttttga tgaaaacaga
     6781 tggttatcca gcgattgcac aaggagcagt cacaactgga ttagtctatt tgattgttgc
     6841 gctgatcatc aaaaagatcg gttcagcatg gctggataaa atcttgcctc caatcgtcgt
     6901 cggtccagtc atcatggtta tcgggttggg acttgcggca aatgcagcaa caaatgctat
     6961 gtataatcag ggcacgtatg atttcaagta tattgctgta gcgctgatca cattaggatt
     7021 aacaattttc ttcaacatgt ggctaagagg ttttctcgga ttgattccga ttttactagg
     7081 tattattagc ggatacatcg tagccttgct gtttggaatc gtggatacta agccaatcgt
     7141 agacgctgcc tggtttgctg ttcctaactt tgagattccc tttgtacaat atgaaccaaa
     7201 gctatatctt ggagcaatca caacaatggc acccatcgct tttgtgacga tgacagagca
     7261 tatcggccat cttatggtat tgaataaact aactaaacgt aatttctttg aagaaccggg
     7321 tcttcataag acgttgacag gtgatggatt agcacaaatc gtggcagcct ttgtcggagg
     7381 tcctcctgta acaagttacg gtgaaaacat tggtgttctg gctatcacac gtgtccatag
     7441 cgtgtttgtg atcggtggag ctgcagtatt tgcagtcatt ttaggattcg taggaaaatt
     7501 aagcgctttg attttaagta tccctggacc agtcatctca ggaatcagtt ttatcttatt
     7561 tggagtaatt gccgcaagtg gaatgaagat tttaatcgag aacaaaatcg attttgataa
     7621 aaagaaaaat ctgttgattg cttcagttat cttagtagta ggaattggcg gactcgtctt
     7681 tgaagtaggc acatttacat tgtcggcaat ggcattagca actgtacttg gcatcatttt
     7741 aaatcttatc ttaccagaaa cttcacgaag cgaagaaaac taaggaggaa acattatgat
     7801 catcaaatca gaacgtatca gcttaaagca tttattgacc gttgaggcac ttactgatca
     7861 agaagttatg ggcttgatcc gcagagggca ggaattcaag caaggtgcta attggactcc
     7921 gcaaaagcgc cagtactttg ctacaaacct ctttttcgaa aatagtactc gcacacataa
     7981 aagcttcgat gtagcggaga aaaaactagg gctagaagtg attgaatttg aagcaagcac
     8041 aagctctgtc caaaaagggg aaacactata cgatacagta ctgacgatgt cagcattagg
     8101 cgtagacgta gcggttatcc gtcatggtga tgagaactat tatgacgaac tgatacaaag
     8161 taaaacaatc cagtgttcga ttatcaatgg aggagacgga agcggacagc atccaaccca
     8221 atgcttgctt gatttgatga ccatttatga agaatttggt cattttgaag gtctgaaagt
     8281 tgccatcgta ggtgatatca ctcactctcg cgtagctaaa tccaatatgc agatgttaaa
     8341 acgtctaggc gcacaagtat tcttctcagg tcctagagaa tggtacgacg atgaattcga
     8401 agtgtacgga cattatatgc cattggatga actactagat caagttgatg tcatgatgat
     8461 gcttcgggtg caacacgaac gccatgatgg aaaagaaagt ttctcaaaag aaggctacca
     8521 tcaggaacat gggttaacag ttgagcgtgc gaagaagttg caaaaacatg caatcattat
     8581 gcacccagca ccagttaacc gtgatgtaga attagctgat tcattagtgg aaggattaca
     8641 gtcgcggatc gttcagcaaa tgagcaatgg agtattcgtc agaatggcaa tcttagaagc
     8701 agtgctaaat ggaaaagcgt aattatagga ggatgaaaaa atgaaaacac tcattaaaaa
     8761 cggtcagatc aatacacgaa aaaatatgac gacaccagca gaaatttgga tagaagatgg
     8821 gcgaatcaaa gcaatcggta caggattctc agaagccgaa tttgatgaag tgtttgatgc
     8881 aaagggacaa ttgatcacac ctggtttagt ggacgtccat gttcatttga gagagccagg
     8941 atttacatac aaggagacga tcgaagctgg gactcgctca gctgctcgtg gcggtttcac
     9001 tactgtatgc gcgatgccga atttaaatcc tgtacctgat acggcagaaa agctgagaca
     9061 agtatatgac atcattagaa aagatgcagt tgtgaaggta ttgcagtatg cgccaatcac
     9121 agaaaatctg cgaagtgaga aattagttga tcaagaagca ctgattgaag aaggtgcttt
     9181 tgctttcaca aatgacggtg taggtgtcca aacagctggg acgatgtatc tggctatgaa
     9241 agaagctgca aaaaacaata aagccctagt agcccataca gaagatgaat ctttattatt
     9301 tggcggcgtt atgcacgctg gcaagaaggc agaagaactt ggtcttcccg ggatcttaag
     9361 cgtaaccgag tcttctcaaa ttgctagaga tctgttgcta gctgaagcaa ctggtgttca
     9421 ttaccatgtt tgccatgttt ctactaaaga aagtgtccgt gtaatcagag acgcaaaaaa
     9481 agcaggaatc cacgtaacag cagaagtttc accccatcat cttattttga tcgacgaaga
     9541 tattcccgaa gatttcggct tttggaaaat gaacccgccg ctaagaggac gggaggacag
     9601 agaagctttg atcgaaggct tgcttgacgg tacgatcgat tgcatcgcta cagaccatgc
     9661 cccccatgga ttagaagaaa aaagccaaag tttcatgaaa tcaccatttg gtatcgtagg
     9721 aagcgaaaca gcgtttcaac tgatctatac tcattttgta gaaactggtc gattcacgtt
     9781 ggaacaagtg atcaattggt tggcagttaa accggctgag atttttggtt tgaatgcagg
     9841 aacgctaacg gttggtgccc ccgctgatgt agcagttttt gatattacac aaacttgcac
     9901 gatcgataaa gaagactttt tatcaaaagg ggagaataca ccttttatcg gctggaaagt
     9961 aaaaggcgaa acacaaatga cttttgtcaa cggaaaactt gtgtggcaga agggagaata
    10021 aaagtatgga acgtttgctt atattagaag atggaacagt tttcgaagga aaggcttttg
    10081 gtgcagatgc tagtgtcttc ggagaaatcg tttttacaac aagtatgact ggttatcaag
    10141 aaactatcac tgatcaaagt tttaacggtc aaatcattac atttacctat cctatggtgg
    10201 gtaattatgg ggtaaataga gatgattatg aatcgatttc tccaacatgc aaaggagtag
    10261 tagtaaaaga acatgcacgt gtagcttcaa actggcgaaa ccaaatgacg ctagattcat
    10321 ttttgaaaca aaaaggaatt cctggaatat caggtatcga tacgcgcgcc cttactcgca
    10381 aaatccgtga agtcggaacg atgaaagcta gtatcgttga tgctggagat tccttcgaac
    10441 atgcatttga tcagctgaaa gcaaccgttc tagcaaccaa tcaagtgcag caagtctcaa
    10501 ctaacaaacc ttatccaagt ccaggaacag gtcggaaagt cattgtcatt gacttcggct
    10561 tgaaacatag tattttacga gaattgagta aaagaaactg taacctcaca gtacttccct
    10621 ataacacaga tgccgagacg attttagcgc tatcaccaga cggagtcatg ctgacgaatg
    10681 gacctggtga tccaaaagat gtgccagaag caatcgaaat gatcaaaaaa atccaaggaa
    10741 aagtaccgat ttttggtatt tgtctcggtc accagttatt tgctttagcc aacggtgcag
    10801 atacctataa aatgaaattc ggacatcgag gattaaatca cccggtacga gaagtagcga
    10861 ccggaagaat tgattttact tctcaaaatc atggctatgc agtcgatgaa gccacgattg
    10921 atccagaaaa actcatcgta actcatgtag aagtaaatga cggaacagta gaaggagtca
    10981 aacacagaga ttatcccgct ttcagtgtgc agtttcatcc agatgctgcc cctggtccgc
    11041 atgatgcact acatttattt gatgaattta tggaaatgat ggacgcagga aaggagcagt
    11101 aaacagatgc cgaaacgtac agatatcaag aaaattatgg tgattggatc tggtccgatc
    11161 atcattggtc aagccgcaga atttgactac gcaggaacac aagcctgcct tgccttaaaa
    11221 gaagaaggat atgaagtggt tttagtaaat tctaatcctg cgacgatcat gacggataaa
    11281 gaagtggcag atcaagtata tatcgaaccg atcacacttg aatttgtatc aaggattctt
    11341 cgaaaagagc gtccagatgc tctccttcca actttaggcg gacagacagg cctcaatatg
    11401 gcaatggagc tggcagcatc aggtatttta gaagaattga acatagaact tctaggaaca
    11461 aagctttctg cgatcgatca agcagaagat cgtgatctat tcaaacagtt gatgaaagaa
    11521 ttgaatcagc caatccccga gtctgaaatc gtcaatacag tagaagaagc tgtagatttt
    11581 gcaaataaaa tcggctatcc tgtcatcgtt cgtccagcct ttactttagg cggaactggt
    11641 ggaggtatgt gtgaaaatga agaagaatta cgtatcgtat cagaaaatgg attaagtctt
    11701 tcccccgcta cccaatgttt gattgaacga agtattgcag gttttaaaga aattgaatat
    11761 gaagtgatga gagattcggc tgataatgca atcgttgtct gcaatatgga aaattttgac
    11821 ccggtgggta tccatacagg tgattctatc gtatttgccc ctagtcaaac attgtctgac
    11881 catgaatatc aaatgcttcg tgatgcttct ctttcgatta ttcgagcact taagatcgag
    11941 ggaggctgta atgtccaact cgctcttgat ccacatagtt ttaattatta tgtgattgaa
    12001 gtcaatccgc gtgtttctcg ttcatctgcg ttagcaagta aggcgactgg ctatccgatc
    12061 gctaagctag ctgctaaaat agcgcttgga ttaacattag acgaaatgaa aaatcctgtt
    12121 acaggaacga cttttgcaga atttgaacct gcattagatt atgttgtagc taaaattcct
    12181 cgctggccgt ttgataagtt tgaaaacgga gaacggcttt taggcacgca gatgaaagca
    12241 actggtgaag tgatggctat cggtcggaat atcgaagagt ctttattgaa agctgtgcgt
    12301 tctttagaaa tcggtacaca ccatatcgaa cttcctgagt tggcagacat cacggacgat
    12361 gacctaatcc aaaaagtggt tcgagcacaa gatgatcgcc tgttttatgt agctgaagct
    12421 attagaagag ggtacaccat agaagaatta gcggatttga caaaaatcga tttattcttt
    12481 ttagataaac tgttgcatat cgtagaaatc gaacagcagt tgaaacagca gccgttggat
    12541 gacaagatat tgaaaacagc gaaacaaaac ggttttacag acaaaaaagt ggcggagctg
    12601 tgggaaataa acgaagaagc cgttcgccag atgagaaaag aaaatgattt gattcctgta
    12661 tataagatgg ttgacacttg tgcagcggaa ttcgaatcaa aaacacctta tttttatagt
    12721 tcctatgaat atgaaaatga aagttcacga tcagaaaagc cctcaattct agtactggga
    12781 tctggtccta tccgtatcgg acaaggggtt gaatttgact atgcgactgt gcattctgtc
    12841 aaagcaatcc agcaggcagg atacgaagca atcatcatga acagcaatcc tgaaacagtt
    12901 tcgactgact tttctatttc ggataaatta tattttgagc cattgacctt cgaagatgtg
    12961 atgaatgtga tcgagctaga acagccgata ggagtaatcg ttcaatttgg tggtcagaca
    13021 gcaatcaact tagccgaacc tttgactgct gctggtgtaa aaatcttagg gacaacgatc
    13081 gaagacttgg atcgtgcgga aaatcgtgat ttattcgagc aagcgctgaa agagttggat
    13141 attcctcaac ctttagggga tactgctaca agtaaagaag aagccgtggt tatcgcttcg
    13201 agaattggtt atccagttct tgttcgtcca agctatgtat taggcggacg tgcgatggaa
    13261 atcgttgaaa accagcgaga tttagaagat tacatggaac atgctgtgaa agcatctcca
    13321 gaacatccag tactagtgga ccgttacttg cttggtggtg aatgcgaagt ggatgcgatt
    13381 tgtgacggtg aaacagtact catcccaggg atcatggagc atattgagcg tgctggtgtc
    13441 cattcagggg attcaatggc tgtgtatcca cctcaaacat tatctgaatc gatcaagaaa
    13501 acgatcgaag attacacgat ccgcttagca ttaggtttga attgtgtcgg gatgatgaat
    13561 atccagtttg tcatccatga aaatgaggtt tatgtgatcg aagtcaatcc tcgagcaagt
    13621 cggactgttc cattcttaag taaggtgaca ggaattccaa tggcacaaat tgcgacgaaa
    13681 gctattttgg gagagaaact aaagacactt ggttatgaaa acggacttta tccagaaaca
    13741 aaagaagttc atgttaaagc acctgtcttt tcttttacga aattgcagaa agttgatact
    13801 tacttagggc ctgaaatgaa atcgactgga gaagtaatgg gatctgacag acaattagac
    13861 aaagctttgt acaaggcatt cgaagcttct ggattacact tgcctgaata tggagccgta
    13921 ttatttacga ttgcggacga aacaaaagaa gaagcactag aattagctaa acgttttaca
    13981 gaaattggct atagtctgat aggaacgaaa catacagctg cttatttcga aaaaaatggt
    14041 ctggtcgttg aaacagtagc gaaaatttct gaggaagcag cagaaaaaaa tgtagtcgac
    14101 ttaataagag atggtaaaac acaagtggtt gtcaatacga ttgataaaga tcggggaaat
    14161 gcttctaaag acggtttcat catccgaaga gaagcagttg aacatggtat tccattgttt
    14221 acttctttag acacagcaga tgcgatcgtt cgagtgatgg aatcccgtgc tttttcaact
    14281 aaccctattt aacggcgagc acatgttaag acaaaaagac gaaaaaccgt tgcaggaggg
    14341 gacagatgaa acaagaaatg atggaaatcg ttcagcaaaa acagctggca ccaaagattt
    14401 ttgaaatgac attgaaaggt gatctagtaa aagaaatgca gcaaccagga cagttcttgc
    14461 atattctcgt gccgcgtgca gacttgctat tacgccgacc gattagcttg aatcatatcg
    14521 accacgaaac aaatacttgc cgtatcattt atcgggtgga aggtgagggg actagagtct
    14581 tctctactct ttctgccgga gaccatttag atgtcatggg tcctttaggc aatggttttg
    14641 atttgtccaa tcttcataca ggagacgaag tatttataat cggtggagga atcggtgtcc
    14701 ctcctctata tgagttatca aaacagttag tcgaaaaagg aattaagcca acgcattttt
    14761 taggttttgc cacacatgaa gtcgtttatt atgaaaaaga atttaatcgt ctaggagaaa
    14821 caagaatcgc tactgacgat ggaacatacg gtatgcacgg aaatgtcggt aatttattgt
    14881 taggaacgaa gacggaacct acagctgttt ttgcatgtgg ttcaaacggg ttattgaaga
    14941 cagtggaaca gctgttttct tcgcatcaaa atgtccagtt atcattagaa tcccgcatgg
    15001 catgtgggat aggggcttgc tatgcttgcg tttgtcatac gccggaagat gaaacaggaa
    15061 caaaaagtgt gaaagtttgt gacgaaggac cgatttttaa aataggagag gtaattatat
    15121 gaatgagaat cctttagcgg tcaaacttcc tggcctagaa ttaaaaaatc ctatcatgcc
    15181 ggcaagcggc tgtttcggtt ttggcaaaga atacggaaaa tactatgatt tgaatcagct
    15241 tggatcgatc atggtcaagg caacgactcc aaatgcccgc tttggcaatc ccacgccaag
    15301 agtggcggaa acaccatctg gcatgttgaa tgcaattggt ttgcaaaatc ctggtctaga
    15361 cgttgttatg cataaacttc ttccagaatt agaggagtac aatgagttac ccatcatcgc
    15421 aaatgttgct ggtgcttgtg aagaagacta tgtagaagtt tgtagcaaaa ttggcgaagc
    15481 accaaatgtg aaagcaatcg agctgaacat ttcttgccca aatgtcaaac atggcggaat
    15541 tgcttttgga acagattctg atgttgcttt tcaattaaca caagcagtaa agaaagtttc
    15601 ttctgttcct gtctatgtca aattgtcccc aaatgtaaca gacatcgttc caatcgctca
    15661 ggcaatcgaa gctggagggg cagatgggtt tacgatgatc aatactcttc ttggtatgcg
    15721 gattgacctt aagacaagaa aaccgatttt agccaatcaa acaggtggac tctccggacc
    15781 agcaatcaag cctgtcgcta ttcgtttgat tcaccaagta gcaagtatat ccagccttcc
    15841 aatcattggg atgggcggcg tgcagacagt ggatgatgtt cttgagatgt ttatggctgg
    15901 agcaagtgct gtagctatag ggaccgcgaa tttcaccgat ccgtatatct gtcctaaact
    15961 aattaaagag ctccctgtgc gaatgagcga actaggcatc gaatcgctgg aaagattaat
    16021 caaagaagtg agagaggaac gtgaacgatg aatcaactag accaacgacc aattattgca
    16081 ttagattttt caactaggca agaggtagaa gactttcttc gatttttccc gaaagaagaa
    16141 aaattgtttg tgaaaatcgg aatggaattg ttctaccaag aaggacctga aatcgtacgt
    16201 tatctaaaag aggctggtca taatgtattt ttagatctga aacttcacga tattccgaat
    16261 acggtcgaaa aagcaatgcg gggattggca aagcttggtg ttgatctcac ctgtgttcat
    16321 gccgctggcg gtattcgtat gatggaggcg gcaatgcgag gattagaaga ggggactcca
    16381 gaaggtggaa aacgtccgct acttctagct atcactcagc ttacttctac aagtgaagaa
    16441 gaaatgcatg ccgatcaact aatcgaagtt tctttagaaa aaagtgtgat ccattatgct
    16501 agctgtgcca aaaaagcagg attagatggt gtggtttctt ctgcttggga agtagaagcg
    16561 atcaaagaaa tggcaggaga cgaatttgtt tgcttaactc cgggtataag accagaagga
    16621 acagtcgcag gagatcagac aagagtagtc acaccttctc aggcaaaaaa gatcggctca
    16681 acttttatcg ttataggtcg accaatcact cagtcaacta atccttataa agcatatcaa
    16741 acgattcaaa cagaatggag ctaacctaaa atgaatgttg aacaaagtat cgcaaaagac
    16801 ttattagaaa ttgaagcagt atttttaaat ccaagtaatc cttttacatg ggcaagtgga
    16861 atcaaaagcc caatttactg tgataaccga atcacgatga gctatccaaa agtccgaaaa
    16921 gagatcgcaa aaggattagc aagcaaaatc aaagaagctt accctgaagt acaagtgatt
    16981 gctggaactg caacagcagg aatccctcat gccgcatggg tagcggaaat actggatttg
    17041 ccaatggtct atattcgaag taaggcgaaa gatcatggga aaggtaacca aatcgaagga
    17101 cgtatatttg aaggtcagaa gatggttgtg atcgaggatt tgatttctac aggcggaagt
    17161 gtcttggaag cagctgaagc agcaaaaaga gaaggtgcgg atatccttgg tgttgcggct
    17221 atctttacgt atgagttgcc aaaaggaaaa gcaaatttcg aaaaagccga aatccctctt
    17281 atgacgttga ccaattattc tgtattgatt gaagcagctt tagaagatcg ttatatcgat
    17341 gagcaagaat taactctgct aaaagaatgg aaaaaagatc ctgaaaattg gcagatttaa
    17401 gcgcgcttga tgaagataga gaaatccgat ttgtgtctcc agaatcatca attggaatag
    17461 caaaaatcca aaaactgatt gcgagaagca tcttttcatc aggtatgaaa agaaacttct
    17521 atcgtgatca gtttttgatt tttttaaaat tcatttttct aagactggtt agcagaaaga
    17581 agagaataat ctcttctctt ttttctatta tatatagatt ttctctattt tttggtaagg
    17641 gagctgtttc aaaaatggta aagagcggaa atgttgcgaa tatcagttat ttaatgatat
    17701 gatattcgga aaaagaaatc tcaaagggag gtaactttta tggggttgaa tgaatttatt
    17761 ttaggattga acaatttaga gacgatcaca agggcacctg gattttttaa atatacagag
    17821 catacagttg cagcacattc gtatcgtgta gcatctattg cgcaagttct aggagacatt
    17881 gaagaagcga atggacaacc gatcgattgg aaactgctct atgaaaaatc attgaatcat
    17941 gactacacag agcgttttat tggtgatatc aaaacgccgg tgaaatacgc aagcaaagag
    18001 ttaagaggga tgctgcaaac agttgaagaa aagatgacgg acgaattcat caaccaagag
    18061 attccagctg agttccaaga agtttatcgc cgtcggttgt tcgaaggaaa agatgaaacc
    18121 gtagaaggag aactattagc catttcagat aaggttgacc ttctttatga atcttttgat
    18181 gaaatcatca aaaataaccc ggaaaaagtt tataaggaga tgttcattga ggcagtgact
    18241 acgattaaaa gctttgaaca tcggccttct gttcagtatt tctttaaaga gatatttccg
    18301 gaactattga accaggaatt ttatggaaaa gaggcgtttt tagaaaccgt ctcaacgatt
    18361 gtaaagaaag aagaaaaata aaaatgatat gagatgcagg gggaagtata gttgaaaaag
    18421 aatatggatg tcgaatgggg ctattctgga gacaaaggtc cggaaaattg ggcgagttta
    18481 tgtgattggt ttgcgagagg tgcggaattt ccattgcagt cgcctattca tttgaccaag
    18541 ggggaagaaa ccaaaataga aggagacacg ctttcctttc attatcaaaa gcaacggttt
    18601 actgacaagg aattcaaaaa cacgattcat ttagtacctt ttgaccaact cagttatgta
    18661 gagttcaaga aagaacgcta ttatttgaca gatattcatt tccacttgcc ttctgaacat
    18721 atcatcaacg gaaaacagga agatattgaa tttcattttg ttcatatgaa cagcaaaaaa
    18781 gagaatctag ttgtgggtgt catgtaccag ctaatggtac aggaaggttg gctgtataat
    18841 caagaaaatg gggaaagttg gaacttagaa aaacatgagc attgggtaaa tccggatgtg
    18901 ttctttccgg agcaaaaaag ccatttccat tatattggct cattgacaac tccgcctacc
    18961 agcgggccaa tccaatggtt tgtaatggat catgtaggaa aactgaacca agagtttctt
    19021 ctccccttcg atggtcagta tgctcgaccg aacaaccgac caatccagcc cttgaatgga
    19081 agagaaattt attatttcga ggagtttgag caatgaatat tcagcagatg aaatacgtgg
    19141 tcgctgtagc aaacaatggt agtttcagag aagcggcaaa gaaaatgttt gtctcccagc
    19201 caagtctttc agctggtatc aaagaattag aaaccgagtt agggatcacg ctgtttactc
    19261 gaacgaatcg aggggcatat ttaacagaag aagggcaaga cttcttaaat cgtgccgaga
    19321 aaatattggt ccagcttgat acattagaaa ctcactatct aacgagtgaa aagagagaca
    19381 atttttcgat cgctgcccag cattatgatt tcttaggtcc tttgaccgca cagctactcg
    19441 aggaattcaa agacgagatc aaacaatttc ggatcatgga gacgacaaca gcgagagtga
    19501 tcgaagaagt caaagagcag cacagtgaga tcggcattat ttatatgaat gagcataatc
    19561 aagctggtat cagaagatat ttacaacaag gagagctaag tggccgcagc attggaaaat
    19621 tcaaaacgca tattttttta aggcaaggac atccattagc tgaacacgca gagatcacca
    19681 aagaagaatt gttatcttat cctcaagttc gttttacaca ggatggaaat aatttcccgt
    19741 atttttatga agaccttatc gagatccctg atcaagaaac agtgatttat acgagtgatc
    19801 gaggtacttt gatgaatatc gttcttgaaa ctgatgctta cgcttcagga tctggtattg
    19861 tgattggagg gattcgcgag cacttgcggt taatcccttt ggcagaaggc gaactaaatg
    19921 aattttatat catttattcg gcgaaacgtc cgctcagtga aatcgctcaa agatttatca
    19981 aagagcttac tcggttactg gaccagcaga gctaagaaaa gcagatgaga acgctgttta
    20041 ttgaacaccg ttgatcaaaa aagaggggtt gtgacagaat tttgtcacaa ctcctctttt
    20101 tgtagcaaac aatttataac taattgctgt tgtgggaaag tacgcaaata tgattctaga
    20161 aacactcatt gattacctgt tttgtttcat agctgtaatt ttttcttctt ctggtgtcac
    20221 gataatcgtc ttttggtttt cataaataac ataaccaggc ttggcgccat ttggttttcg
    20281 gatatgcttt acctgtacaa gatcaaccgg cacttgggca gagtatcggt attttgaaaa
    20341 ataggcagct aactcggcag cttctgtaat tgtttcatct gagggcttgt cacttttaat
    20401 gatgacgtgc gaaccgggta tattttttgc atgaagccaa tagtcggttt ttttagctgt
    20461 cttcattgtc agctggtcat tttgaagatt gtttttacca acaagaatga gcgttccgtc
    20521 agaagaaaag tattgatcag gctgagatgg tttcttacgt ttttgttttt ttaagttctt
    20581 ctttttcagg tatccttctg aagtcaattc ttctttaatt gcttcgatat ccattgggcc
    20641 ggcaatttcc agttgagata agacagattc aagatattgg atttcatctt tggcttcttg
    20701 gatttgttcg ccaatcagct taacagcgtt cttcagcttt tggtatcgat ggaaatattt
    20761 ctgtgcattt tgatttggtg tcaatgccgg atctagtgct attttgattg gccgatcttc
    20821 ttcataataa ttaggtaaaa caacttcttt tgcgccgcgt ggtacttgtg tcagaaaagt
    20881 agtaagtagt tcgccatttc tacggtaatt ctcagcgttt tcggattctt tgagtgtttg
    20941 ttcacgtttt ttcaatttat ttttatttcg ttttagttca ttttcgattt ttcgaatcag
    21001 ttctccgcct tgttgttttg cccgatcttt ctctgctttt tcgtgataat agctatctaa
    21061 taattgactg agtgttggat aagctgttac agcagatgcc tgttcggaca atacctgata
    21121 agcaatcggt gtgaaaaaat ctttattttc cgtttcgtaa aaagtaggaa taggctgatg
    21181 gctgatggct gagaaaaatt cgtcccatac aaccatcttt tcattaggac gttccagtaa
    21241 tcttttggtc agctcttgag cgatatcgaa accgattccc tgaaactgac gttggattgc
    21301 ttttggatct agttcagttt gggaaagccg ctgaaagatt ttttctttct cggagctaaa
    21361 tgggttcaag acctcttgtt ttggcggagc aacatattct acaccaggaa gcaaagaacg
    21421 ataagtgttt tgagagcttc caatgtgttt gatcgcatcc agtatttttc cagtttctcg
    21481 attaactaag acgatcgtac tatgacgtcc cattaattca acgatcaata tgatattttg
    21541 tagatcaccc aactcgtttc gtttagcaaa gtggaaatgg atcactcgat cattttcgat
    21601 ttgttcgatg tcttctaaaa tagcgccatc caaatatttt cgaagcatca tcacgaagtt
    21661 aggcggatta tcaggattct gataatcgat ttgggtaatc tgtacacgag cataacttgg
    21721 gtgcgcagat aataataatc gatggttttt gccgcgtgaa cgaatcacca gcacgatttc
    21781 attttcataa ggctgatgga ttttcgagat ccggccagat agtaacgttt cacgcaattc
    21841 gttgaccatc gcgtgggtaa atacaccatc aaaggacatg gttcattcct catttctttt
    21901 cataagtata ttcattataa cgaaaaaaag aagaaaaacc aatttggccg aatagtaaag
    21961 caaaagtcta gaaaaatctt ggactaatta cataaaaaac agtgtttcta tcgttgaaat
    22021 ccaaattttt aggtataata aaaaatagga gaaaaagtag aaaagaaggt gtttgttatt
    22081 tgggaagtag ttaaatcgag agtacaagaa atgaatgaac atttctcagt gtcattcgca
    22141 gaaaatccaa gtaagtattt tcgacgtctt gtttttctgc tcatcttatc attgctgtta
    22201 acagttgcaa tgtttttatt gccggaaaaa atccgagaag agcaagtaaa tagtctcgtt
    22261 tattcgtcca taccaaccag caaattgaaa ccttttgatt acagcaaagc tgatcagaaa
    22321 atcaaacaaa gccgggcgat atcggtgatg ttcagcaaac cgaacgggaa aacctatcag
    22381 gacatgcttg atgtttttaa tgatcctgag cttatggaag aattgaatcg accgatcttt
    22441 tattatccga tcgtttatga tgtacatgaa ctggaacaaa aatataacat acgaacggat
    22501 gaagtgacat ttattttctt tgatggagga aaagaagcaa atcggataac tgcgggtaag
    22561 ggaggaataa ctgattttga taaggagttg atccctgaac tgaatcgact tccgttagcc
    22621 aatatcaaac aattagaaga agaattggat agaacgggag taacttccca aaattccgag
    22681 attgctaatt gagacattgg cgcgatgaaa cggtgggtct atatagcgct atttttaatt
    22741 atcagaacaa gtgatgtttg atcttcaaaa taacaaataa aaaattaaaa aatatagttg
    22801 acgaaattca aaaattcgat tagacttaaa tcaattctta aggaaagggt gacctgtaat
    22861 gaacttatca aaacgtattt cagcagaaaa caaaaaaaat tggcgcagct ggcgtagata
    22921 agttgtatta atggtgagac tttcccatag atacaacttt ttagagtgca tttctaaagc
    22981 ttgtatctat gctagacatg aacattgact gccgcataga ttagaatcta tgcggttttt
    23041 tgtgtaaaga caatgaccgc tttggccatt gtcttttttt gtagactgga tacttgcctg
    23101 attataataa ataaaaagat tgactttgca ggaggaaaaa aatgaaaaac aaacgattaa
    23161 taacagttgt agtaattatt ttagtttatt tagcaggtgc atttatttat ggaaaagcct
    23221 caggcgaaaa agaaagtact tcgacgaacc agacaaaaaa aacaacagtt gttggggtat
    23281 tgcaatacgt gagccatccg gcattagatg agatttatcg aggaatcaaa gatggtctga
    23341 aagaagaagg attaaaagaa ggagaaaatc ttgagatcaa atttcaaaat ggacaagccg
    23401 atcagagcaa gctagccaca atgagtcaac agctagtaca atctgatcca gatgccttga
    23461 ttggaatcgc aacacctgcc gctcaatcat tagcaaacac gacttcatcg atcccagttg
    23521 tgttaggtgc tgtaacggat cctgtggggg ctggattagt cgcttcttta gagaaacctg
    23581 gtggtaatgt aacaggtgtt tctgatcagc cgcctgttac ttcacaaatc aagcttgctt
    23641 ctgatttgtt gccaaatgca aaaaaagtcg gaatcttgta tgcttcttca gaggataatt
    23701 caaaatacca agtagcacaa gcagaagaag ctgcaaaaaa agaagggtta gaatcagtaa
    23761 aatatgcggt gccttccact aatgagattg cacaaacggt tcaagtgatg agccgacaag
    23821 tcgattttat ctatgttcca ttagataata ccattgccaa tgcgatgcaa acagtggtaa
    23881 aagaagcaaa caaagcaaat atccccgtca ttccatctgt ggatacaatg gtggaacaag
    23941 gtggactagc aacaatcggg atcaatcaat atcaattagg gcttcaaagc ggtaagatgg
    24001 cggcagaact tgcgagtgga aaagaaaaac cggaaacgac gcctgtttat acatttgatc
    24061 aaggggatac agtgatcaat caatctcaag ctgatcgctt aggaatcatt gttccccaat
    24121 cgatgaagga aaaagccaaa atcattacag atgactcgca gcaagaaaca tctaaggagg
    24181 atgacaaatg atcgtatcag ctatttcaca aggcatgctt tgggcaatat taggattagg
    24241 gattttcatg acttatcgga tcttagactt tcctgatatg acgacagaag ggtccttccc
    24301 attaggtgct gctgtctgtg tgacagctat tactcatggg gtagcgccga ttgtagctac
    24361 attattaggc gtaggtgctg gcatgatagc aggactcgtt acggggttac tttatacaaa
    24421 aggaaagata ccggttattt tagcagggat tttagtgatg tcaggattga actcagttat
    24481 tttgtttgtg atgcgaacac caaatctttc tttattgaat catccgattc tacaagacag
    24541 cttccatcgc tttgggttac ctgattactt tgacacagtc cttcttggct tcatctcttt
    24601 gggcatcttg attgctgtac tgctattctt cttcaataca gatttgggtc aaggctatat
    24661 tgcgacagga gacaacgaaa cgatggctcg gtcgttaggg ataaaaaccg atcgtatgaa
    24721 aattttaggt ttaactttgt cgaacggtgt cattgcatta tccggtgcgc tgattgccca
    24781 gaacgacgga tatgcagatg taaataaagg gattggcgta atcgtcatcg gtcttgcttc
    24841 catcatcatc ggtgaagtga tcttccaaga attgacgcta gcagaaagat tgattgctat
    24901 tgttgtcggc agtatcattt atcagctatt gattttagta gtaatcaaac tagggtttga
    24961 cacgacttat ctgaagttgt tctccgctat catcttagct atttgtctga tgatcccgca
    25021 attgaagaaa gcactgaact taaaaacggg ttttgagaag gaggcatagt aatgactgat
    25081 atattgacaa tcaaaaatgg cagaaaagtc gtaaacaatg gaatgaatga agagaaagta
    25141 ctattggatg aaattaacct tacaatcaga gaaggggact tcatcactgt ccttggcggg
    25201 aatggtgctg gaaaaagcac actgttcaat acgatcgctg gtacgcttca actcagtcaa
    25261 ggatcgatct cttttaaaga tcagctgatc acaaaagatt cagaagaaaa aagagcacga
    25321 ttcctttctc gagttttcca agaccccaag atgggaacgg ctcctcggat gacagtagca
    25381 gaaaatttgc tacttgcaca aaaaagaggt caaaaacgga aattccgttt acgtaacttg
    25441 aaaaatcaaa aagaagattt tttcagacta tgtcaagaag tcggtaatgg cctagagcaa
    25501 cacttggata ctccagcggg tgaattatct ggtggtcaac gccaagcatt aagcttgctg
    25561 atggcgacga tcacagctcc tgagttacta ttattagatg aacatactgc agctttagat
    25621 cctaaaacag ctaaagcatt aatgcgatta acaaatcagc ggatcaatga acaaaaattg
    25681 acttgtctca tgatcacgca tcgaatggaa gacgccttga aatacggaaa tcgtctgatc
    25741 gtactccaaa aagggaaaat cgttcaagat atttctgaaa aagaaaagca acatttgaca
    25801 atgggcgacc ttttgcagtt ctttgaagaa gtagtggatt gatttcgaca gaaaaattcc
    25861 tttcagtaga aggacttgaa aactcattga catcttcctt tgattcatcc tatacttaaa
    25921 acaataaaca attaaacata tttgggtgtt ttgtaggatc acgtagtcaa tgattttaac
    25981 aattaaatag aatattcttt gctctgacaa gtaaacgatg agaaaagaat ttgctacttt
    26041 gccacctaga cgtttcgtac tttttcgtat caaacgct
//
LOCUS       DAYYHB010000035        25929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000035 DAYYHB010000000
VERSION     DAYYHB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.036"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHA6B_001397"
     CDS             185..1183
                     /locus_tag="ACHA6B_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8948981.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHA6B_001398"
     CDS             1295..1804
                     /locus_tag="ACHA6B_001398"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8948982.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHA6B_001399"
     CDS             complement(1834..2493)
                     /locus_tag="ACHA6B_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8948983.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHA6B_001400"
     CDS             complement(2495..3259)
                     /locus_tag="ACHA6B_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8948984.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHA6B_001401"
     CDS             complement(3267..3944)
                     /locus_tag="ACHA6B_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8948985.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHA6B_001402"
     CDS             complement(3981..5366)
                     /locus_tag="ACHA6B_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8948986.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="ACHA6B_001403"
     CDS             complement(5335..5589)
                     /locus_tag="ACHA6B_001403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948987.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHA6B_001404"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA6B_001404"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA6B_001405"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA6B_001405"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8948988.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACHA6B_001406"
     CDS             6305..7162
                     /locus_tag="ACHA6B_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948989.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACHA6B_001407"
     CDS             complement(7243..7578)
                     /locus_tag="ACHA6B_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8948990.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACHA6B_001408"
     CDS             complement(7645..9015)
                     /locus_tag="ACHA6B_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8948991.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9278..10357)
                     /locus_tag="ACHA6B_001409"
     CDS             complement(9278..10357)
                     /locus_tag="ACHA6B_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8948992.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(10482..11018)
                     /locus_tag="ACHA6B_001410"
     CDS             complement(10482..11018)
                     /locus_tag="ACHA6B_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8948993.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            11327..12808
                     /locus_tag="ACHA6B_001411"
     CDS             11327..12808
                     /locus_tag="ACHA6B_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8948994.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(12911..13618)
                     /locus_tag="ACHA6B_001412"
     CDS             complement(12911..13618)
                     /locus_tag="ACHA6B_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8948995.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13697..14035)
                     /locus_tag="ACHA6B_001413"
     CDS             complement(13697..14035)
                     /locus_tag="ACHA6B_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8948996.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(14146..15108)
                     /locus_tag="ACHA6B_001414"
     CDS             complement(14146..15108)
                     /locus_tag="ACHA6B_001414"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8948997.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(15123..16454)
                     /locus_tag="ACHA6B_001415"
     CDS             complement(15123..16454)
                     /locus_tag="ACHA6B_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8948998.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(16462..17142)
                     /locus_tag="ACHA6B_001416"
     CDS             complement(16462..17142)
                     /locus_tag="ACHA6B_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8948999.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(17361..17840)
                     /locus_tag="ACHA6B_001417"
     CDS             complement(17361..17840)
                     /locus_tag="ACHA6B_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8949000.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(17967..19646)
                     /locus_tag="ACHA6B_001418"
     CDS             complement(17967..19646)
                     /locus_tag="ACHA6B_001418"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8949001.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            20194..21696
                     /locus_tag="ACHA6B_001419"
     CDS             20194..21696
                     /locus_tag="ACHA6B_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949002.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            21709..22371
                     /locus_tag="ACHA6B_001420"
     CDS             21709..22371
                     /locus_tag="ACHA6B_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949003.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(22472..22675)
                     /locus_tag="ACHA6B_001421"
     CDS             complement(22472..22675)
                     /locus_tag="ACHA6B_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8949004.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(22786..23970)
                     /gene="iadA"
                     /locus_tag="ACHA6B_001422"
     CDS             complement(22786..23970)
                     /gene="iadA"
                     /locus_tag="ACHA6B_001422"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8949005.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(24127..25866)
                     /gene="yfcC"
                     /locus_tag="ACHA6B_001423"
     CDS             complement(24127..25866)
                     /gene="yfcC"
                     /locus_tag="ACHA6B_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8949006.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         7529 a         5555 c         4533 g         8312 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttcgt aaatttcaag tttaagttag ccacccatgg
     9121 tgacccggtt gctcgttacc accaaccgtc taatttttct aatcgagtgg gcccttgaca
     9181 atgagctgag gcatggtagt ctcccgactg gctggccccg gccagctttc cagacacaag
     9241 ccatgccgag ggaggctcgt tgtcaagggc ccacgtgtca agccgtcaac cgttcctgcc
     9301 taaatgcctg gacgaacgtg tcatgcggcg ttgcatagcc caaaatcttt cgcgggtaat
     9361 cgttcatcca acgctgaatc cgcaagattt ggttttcgct cacgttgccg attgggctgc
     9421 ccttggggat gaaacggcga atcagcttgt gttggttttc gctcgtcccg cgctcccagg
     9481 aagcataggg gtggctgaag tagatgtttg ccacatcctg caaggcatcg tgcagtcccg
     9541 cgaactccga gccgttgtcc gaggtaaccg tcttgaacag ctggccgaag tacgcaccag
     9601 cccgttctct cagctgtcgg accgcttggt tgaccgattc ggcatccttc ccggccagct
     9661 tcagaatgac ctcgaagcgt gtctgccgtt ccaccaacgt cagcagcacg gcatccgact
     9721 tggtcttgtt tcctacgacc gtgtcgactt cccaatggcc gaaggttttc cgggtaccaa
     9781 ccgcctccgg gcgctcttca atggatttcc ctagcacccg tttattcggg cggttgccat
     9841 gacgggacga tttcggtttg cgggacagtt tttctagcag gtccatattc ttcgttttca
     9901 tgatgccacg gtcgatccac tgatagaggg tggtggtccc agggataatg gccgggtcga
     9961 acaattcgtg ttttctggca aagcccacca ccacgtccgg ggaccatttt tccaacagca
    10021 tcatgtcgtc cgcccattca atgaaggcgt ccgtgtcggc ccataagggc cgtctgccgc
    10081 agttcaagcg gttcttctcg taggccgcct gcccggcatc ggcgtcgtag acgtcatgat
    10141 aatagtcgta gaccttcccg ttctgattct ggcggttaag ctgagtaacg gtcccgcgtt
    10201 tgatttcgtt gtttatggtc tgcggcacgc agcccaggac atccgcaacc tgacggttgg
    10261 aatagttttc ctttttcagg atggcgattt ggcaacgttc tgagtaggtt aggtgttttc
    10321 ccttgcgtga aactgtgtta tcatggtttt gcgtcatgtg aattcatcct tgtctattga
    10381 gagttgtggt aacttcaata taacatggaa ttcacgtggc gtttttttat ttccaaatgg
    10441 ctaagttaat tataaaatcc gccacttttt catgttgctg cctaaggtga ctgttccttt
    10501 aagatatttt ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt
    10561 tcaagaaaat cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc
    10621 aattcaagcg acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc
    10681 cgcttaaata gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca
    10741 tggatagaaa aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat
    10801 tcagctaagt agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg
    10861 ctgattgatt tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac
    10921 tctcttgcag taattgactt cattccatat tcttccacgt ataccatact tttgaacagc
    10981 aaatattctt tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat
    11041 ctacccataa ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat
    11101 atttagttat atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa
    11161 aataccctag gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat
    11221 ataaaaaaga aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca
    11281 gtttttgtct aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc
    11341 aagaaaaaaa gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca
    11401 agaattatcc aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat
    11461 aaatgtatca ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg
    11521 agagatccaa accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa
    11581 attatgctac ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc
    11641 acaaactgct gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct
    11701 cgaaaaactg acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc
    11761 tgtaaaaaat tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt
    11821 ttttctttca gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc
    11881 actaaaaatg aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat
    11941 ctacttgctg ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac
    12001 ttcttctgaa atcactgaat tgatggactt attgcagcat acacatgatt attcagaata
    12061 ctttacttct ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt
    12121 taattatttt ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat
    12181 tggtttttta ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac
    12241 ttacttaact caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta
    12301 ttttcttgtg ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc
    12361 tacactttgc tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa
    12421 attaagagaa aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca
    12481 ataccttagt caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat
    12541 ccatttgcag ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca
    12601 gttatttcgc aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat
    12661 tacagatcag catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca
    12721 caatacagtg ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg
    12781 ttatgagaac aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat
    12841 aaatggtatt caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa
    12901 cttctttatt ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca
    12961 ggaatgtcga aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg
    13021 ccatcggtac ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc
    13081 gagacgcgtt aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag
    13141 cgaaaatccc gatattgatt aacgtagagt tccagctaag caatacacct accataatca
    13201 aggggaacga aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca
    13261 atgggacata acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta
    13321 tcgcagcaac tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg
    13381 aattgtagtt atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt
    13441 gactttgtaa tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt
    13501 gatcgtattt tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc
    13561 caagaatgac caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt
    13621 tcgtttcact ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa
    13681 agaccttgaa tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa
    13741 atccttctac cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt
    13801 ttgttccttg tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca
    13861 cgctatcgcc ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta
    13921 tgacagtttc ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc
    13981 ctcgatcttc atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct
    14041 ttctccttta aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat
    14101 acatttgtga tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct
    14161 gctcttatac gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa
    14221 atcttttgca ttcgcaccac ttgctaatgg atggcctcct ccatgatggc gtttagccaa
    14281 ttcattgatg atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg
    14341 aacaaaaatc ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc
    14401 tgtctctgaa tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc
    14461 tgcaccattt tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt
    14521 catcggcatt tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa
    14581 tatcgaagca atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc
    14641 tccgacgatt cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg
    14701 aaacatcaaa gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca
    14761 cacaagatct ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc
    14821 caactgatat ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag
    14881 cgctccttgg tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc
    14941 aatcgtttca cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc
    15001 taatcctact tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat
    15061 tttatcgtat ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct
    15121 ttctaagaac gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca
    15181 ataaacactt cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga
    15241 atgtctaatt cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc
    15301 aagatatttc gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg
    15361 ctaagtacgc caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat
    15421 ttgaacttag ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt
    15481 ccagatatct gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc
    15541 gctttcatta catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc
    15601 ggcatgactt ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt
    15661 ttctttacac tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc
    15721 cctaaaacat cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga
    15781 aaacgcgaat gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc
    15841 tgcaaataat gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt
    15901 ttgatcaatt ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa
    15961 gaggtgcgta agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta
    16021 ttgaatcctc ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc
    16081 gcccggttgc cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta
    16141 gtcatcgcgc cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa
    16201 acgtcccctt caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt
    16261 aatttgcgtt cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt
    16321 tctgcatctt tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg
    16381 cttcttacag aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca
    16441 tgttttgtcg ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc
    16501 ttcactatct tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt
    16561 attgtaatgg attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc
    16621 ttctggcccc atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc
    16681 ttctgcaagc aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt
    16741 tccttctgct tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac
    16801 ttcatagctt gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg
    16861 ttttccaccg atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat
    16921 ctcaacaata ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat
    16981 gtggtcacta tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa
    17041 gtctgtcaat ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat
    17101 ttcaattgtt aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt
    17161 tctctgtatc ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag
    17221 ctgctaaata tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat
    17281 aatacaggat gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc
    17341 ttagcttctc ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt
    17401 tctaaatcgg ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact
    17461 tcttcttttt cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt
    17521 attccaatag gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt
    17581 ccaggaaaac caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga
    17641 tcgttcaaag gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt
    17701 ttacatatca aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct
    17761 accacatgac taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg
    17821 acttttgttt gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag
    17881 ggatgctgta ttcaaaaatt atttgttttt cgcaaaaaaa gagcaagacg agcactctat
    17941 agagaggttc atcttactct ttttgtttat tttttatttc gacgttgagt agcttccata
    18001 atgactggca agatcactgg gcggcgtttt gtttgttcaa ataagtaacg gcctaattga
    18061 tctcggatgt cttgctttaa tttgctccac tcaaaatcat tgctttctaa atgtttctca
    18121 acgattgttt caatcatatt gctgctttct ttcatcagat cacggctagc ttttacgtaa
    18181 acgaatcccc gtgaagtaat ttgaggtttt gaaatgattt tcttttctcg ccgactgata
    18241 gtgacaaccg ctacgaaaat cccgtcttct gacaagattt ttcgatcacg taaaacaatg
    18301 tttccgatat cacctatgcc taatccatca atcattacat tttctgcact tgtagttcct
    18361 gcaacactca tacgcttgtt cttatattcc agcacgtctc cgcgtccagt aatatagata
    18421 tttttgtatg gcatccctac tgcatgagcc aaatctgcat gagcagctaa ttcacgatac
    18481 tctccttgaa ctggcacaaa atatgttggt ttcatcaaat tgatcattaa ttgcaagtca
    18541 ttaggattgg catggccaga aacgcgcata ttctcagaga tcaacttcac tgttccacca
    18601 gctcgataaa tgatatcttc cgttttcgcc acgatggttt ccatcgcgat gcttggcgta
    18661 gtagtgatat aaaccaaatc tccttcttca atcttgacat tacggtgtgt accgtttgcc
    18721 attttttgca gagacttgat gggttcaccc attcggccag tttctaaaat gatcagttct
    18781 tctggatcat agtttttcat gtctttcaac gtaacaagca gatcttcact tggtaattga
    18841 agtttttcta aacgcatcgc tgtgcgaata attcgttcga aatcttgacc agtcaagaca
    18901 acttttcgtt gtgctttttc tgctgcatcc aatacttgtt gcactcgttg caaattactt
    18961 gctacacaag cgacgatgat acgcccttcc caatatttga tcgtatcaaa tacttcatct
    19021 gctatttggg cttcagaagc tacaggctga ggattttctg catttgccga ttcacttagt
    19081 aaggctaaga ctccttcttt gccgatttcc gccaatcgtg cataatctgt ttgatacatt
    19141 gggatagccg tttggtcaaa tttaaaatct cccgtataaa cgatactacc ttcttttgtt
    19201 ttcagattga tccccactga atctggtatc gtatgagtgg tacggaaaaa gctcactact
    19261 gtttcaccaa agtctatttc tgtatgctca tctaccacat ggaaatcctt aaatcctttg
    19321 gctgcttcat tacgactgac attcaatttt gctaactcga tcgtcaactc tgtcccgaat
    19381 actggtacag aaatttttga caacaagtaa ggaagtgccc cgatcgcatc tgcatgtcca
    19441 tgtgttaaaa agacaccagc tacacgatca atgttttctt ctaaatacgt aaaatcaggg
    19501 attacgacgt cgatccccaa caactcattt tcaggatatt ttagcccaca atctaaaata
    19561 aaaatatcct cgcccacttc tgcgacatac atgttttttc cattttcacg aacgccgcct
    19621 aatgggataa ttttaatggt actcacaaac ttcacctctt cttatgacat ccaagtttaa
    19681 agatgtcgat tttcttcttt atctaaatat atgagccatt ccaatgaagc atatgcaaaa
    19741 tcactatttc tcaacttatg gagaatcagc aggaaaatta ccagcacatc cgcgttctga
    19801 cagttggatg ggcattttat tttggatata cttcattaac aaaatggatc ttttgcgaaa
    19861 cgctatagcc agaatactta tataagtatt cagctgttcc ccattattat acaacaaata
    19921 aactaaatcg ctagtatcag caattgttag aacaactatg cacgtaaaaa tccctctgta
    19981 gcatctagga tttcttttgc tattcctctt tcatttcttc ttcgtcggtt tgaaacatac
    20041 ttttttcatt ttaccacatt ttttgcaata actatagtga ctttttatat atacagaacc
    20101 tcatgttgat ggtgtgcaaa cagcataaga tttaagctag ctttaagata ggttgtctat
    20161 tatcaaatgc atctactagg agggatgcaa aatatgaatt ttatgaaacg tgcatggaaa
    20221 agtacaaaag taaaatgggg acgttcattg ttattgtttg ccgtgttcac agccattctt
    20281 atttttgtgc tagctggcct cacgattcga agcgctgcag aagaagcagc gaatcaagca
    20341 caaaaagaag ttggagcaac agtgacttta agtgccaatc gagaagcttc atttaaaaaa
    20401 caggaacaaa cttcttcaag cggttctacc actgaagaac gtccagatcc aggcagtttt
    20461 tctcttacac cagtttcctt aacggacgcg gaaaaaatag ctgaattaga taatgtaaag
    20521 agttattcct ttgaaagttc cgcctcagct atggcaaaag atggaataac tgccatcact
    20581 agttctgatt caagtgaaga cagttcagaa actactgatg caggcggtga acctgctgga
    20641 ggaatgggcg ggaatccgca aatgactcaa gcagactttc aaatcagtgg tgtgtcagca
    20701 actgcacaaa atagtaattt ttctgatgga acagcaaaaa tcacggatgg tgaaggaata
    20761 gatgacagcg acaaaggcac aaacaatgta gtcattgatt ctactctagc agaaacaaat
    20821 gacctggcag tcggagatac tttcacaatc acgagtactg aagatgaaga taccacttat
    20881 gaaatgacga tcaaaggaat ttatgaatcc agcgaaacaa gcggcagtat gggtatgaaa
    20941 ttcaacttca tgaatccgtc gaatacactt tacacttctt acacttttgc aaatgaacta
    21001 aatgggacat ctgacgataa cacgatagat tctgctattt acactctatc tgatccagat
    21061 aaaatggatg agtttctcaa agaagctgag aagctgatcg acacagatac atttagctta
    21121 caatcaaatg attccatgta tcagtcaatg ttggaacctt taaacaacgt ggcaagtttt
    21181 tctaaaaatg ttgttttact agtggctgta gccggtatta tcatacttac tctgatcatt
    21241 atgattacca ttcgggagcg tcggcatgag ttaggcgtct tgctatcttt aggagaatcc
    21301 agaagtaaaa tcattctcca attgtttacc gaagtggcta tttgtatgat tcttgcatta
    21361 ggagttgctt cgcttagcgg aaacgtcgtc gcgaatgccg tgggacagca gctacttgac
    21421 caacagacag aaaccacgac acaaaaccag caagctcctg gcggtggaca aatgccaggt
    21481 aacagacaag aacaaggcgg tcaacgcgat ggtcaacaag gaaccacgaa taatccattt
    21541 gctgtttctg atcaagtcag cgaattggaa atcaatgtca aacctgcaca attgggacta
    21601 ttagccggag tagcatttgg aatcagtctg ttttctgtct ttttagcttc tatagggata
    21661 ttaagattaa acccaagaaa aatcctatta aactaaagga ggaatttgat gttacggaca
    21721 gaacaattgg gattttggta tcaagatccg caagatgcgc tttataaaga tgtagaactg
    21781 aatttcgaag ctggaaaaat gtacgctatt ttaggagcaa gtggttctgg aaaaacgact
    21841 tttctttcgc ttatttctgg actagatgca ccaaaagaag gaaaggtata ctatcaagat
    21901 cgttcactga acaaaatcgg gttaagaagt tatcgtaaaa atgatgtctc gattattttc
    21961 caagcttaca acttgcttcc ttatttatcg gctctagaca acgtcatagc agcaatggaa
    22021 atcactaaga ccaagcaaga gaattatcga caagttgctt tagatcgact ggctcgtgta
    22081 ggaattgatg aaatattagc aaaaaaacag gtgaccaaac tttcgggagg tcagcagcag
    22141 cgagtcgcta tcgttcgtgc tatctgctgt gaacatcaac taatcgtagc ggatgagcct
    22201 actggaaatc tagatgaaac aacttcacaa gatatcgtca aacttttcca agaaatcgcc
    22261 catgaacaaa atcgctgtat tatcatcgtg actcatgaga ttgaagtagc aaaaagctgt
    22321 gatgaagtct ttgaactaaa aaataaaaaa tttcaaaaaa tcgaagttta aaaaacagga
    22381 taatgatcaa ataaaaaatc cgaaccatat ctgtctcgca gatatggttc ggatttttta
    22441 tttgcttgat tcaattagta ataagtggtt tttatttttt ttcgtctttt cgagaaaact
    22501 tgtctttgac ttcgtcgcct aaatttcgag ctttttcttt gatgtcagca cctagtgatt
    22561 gtgctttccc ttctgtttct ttgctcttgt cgtctgttgc tttgccgtat gcttcttttc
    22621 cttcaccgac tactttatct ttcatatctt tagaacgtcc ttttaaatct gccattgtgc
    22681 ttcctcctct tagtttgttt attgatctac cttcagggta acatcaacaa cagatacaga
    22741 caaacagaac gctcgttttc taaaaaagat agttagagct gttgattaaa aacttttttc
    22801 gaaagtcccc catactttga cttctttttc ttggaccatt atttctcctt tagcgataac
    22861 tgtatcaata tcaagcgttt ctttatccaa gatacacaga tctgcatcag caccaatttc
    22921 gatttttcct tttttatcca atttcaatat acgtgctgga ttactggtta tagctcgtaa
    22981 ggcgatttcc aaagggatac tttctttttg cacagcttct ttgatcccta ctagcaatgc
    23041 cttggcactt cctactccta accctaggaa atgattgttt tcatcaaaat aaggcaagct
    23101 tccttgtcca tctgaggtca tcgtgaaatt atctaaagaa acatcttcct cgatcaatcg
    23161 tttcagtgct ttgctgaagc gaacttcacc atctgtcttc tcccaaaaat caggatcttc
    23221 gctagcagtg aagtcaatcg tgccaccacg tttggcaaat gccacgcaag cttcaaataa
    23281 ttctggtgtg cgatttgcat gagtaggcaa aaattgtgtg atcggaattt ctgtttcttc
    23341 tacgattcgt gtcaatagct ctagctttct ttttcctccg cctaagtgga cattgatgat
    23401 cccagctttt ccggataaca tgcctcctac acgagcatct gctactgcac gagcgaactc
    23461 ttcaaaactt ggctgagaag aacggtgatc ggatactgcg atttctccga ttccgataat
    23521 tttatcaatt gtcaaaaagt ctttgatgat agaatctgtt actgttttta ccggcaatcg
    23581 gtacgaacct tcgtatacat aagtggagat tccttctgct tccagacctt tcgctttgct
    23641 gatcaacgca gtcatatctc taccttctcc gtctgttccc aaacaaccga caactgtcgt
    23701 tacaccagct gtagtcaatt ggctcaatgt gacttctggc gttcgatttt gataaccgcc
    23761 ttctcctcct ccacctaaaa tatggaaatg acagtcaatg aaacccgggg ttagtacttt
    23821 tccctcacct gaaagttctt ctacttccac tccttcgtag ccgccttcta gctgttcttc
    23881 tacagccaaa atcttcccac cggcaatcag tacatccatt tttcctaagt aatctggtgc
    23941 aaacacttca atttgtcgaa tgattttcat acatttttcc tctttcttta agtataactg
    24001 atgataggat cttaaaaaat aatccaaacg ataggatctt tactcctttg attttaccgt
    24061 aaaatgacgg ttcttggagt ccttatctac tgtttggatt acttttttat ttacaggtct
    24121 ccagtactac tgcagaccta ttgcaatggc aatcatgatg aaaatagttc ctaaaacaaa
    24181 caggaatcct tgcatcttga tttggaatct tgcccatttt gcccattcga tccgtgcaac
    24241 acctagcaca ccaatcaagc tggctgatac gggtgtaaaa gcgtccataa aacctgaacc
    24301 aagctgataa gcaagaactg ctacttgtct ggatacacct actaaatctg ccagtggagc
    24361 catgattggc atagtcaaag ctgcttgtcc tgaatttgat gtaacaacca agttgaacaa
    24421 actttggaag atgtacatcg cccaagcacc aatgacagct ggaacaccgg tcaacgccgt
    24481 cccgatccca tgcaaaatcg tattcaaagc tgaaggaaca gatgcatcag atcctcccaa
    24541 gacaagaaga atccctttgg ccatacctac tacgattgct gtaccagcta aatcagctgc
    24601 accagattgg aaagaagaag cgatgtcatt gactttcatt ccatctaatt tgaaaagaac
    24661 agcaatgatt ccagcaacta atcccatcac aaagaattga gaagcgattt ctggaatgta
    24721 atagcctttt tgagtcacgc cccaaacgat ccagacaagg acaactaaca tctctaataa
    24781 gatcaattta tgcccaagca tgaatttttt attttcatca gatgtctttt tgatatgaga
    24841 acggaaataa tcatctgatt tgtaagtcaa cgaactttct ggttttttac ggattttttc
    24901 tgcatatatc atcatataac cagcggctaa tgcagtaaca actacccaca taatcaatcg
    24961 gaatgtcgca ccagacaaca cggggattcc agcaatccct tgagccaccg ctacgctaaa
    25021 cgggctcatc caagaggtcg catttccgac ttgagatgcg acataagtca cggttactgc
    25081 tacgatcgag tcatatccta acgcaatgac aaatggaacc atcaccattg agaatggaat
    25141 tacctcttct gccatcccga atgtagcgcc accgaatgaa aaagcaaaga ataatagcgg
    25201 taaagctaaa cgctccagtc ccttagtttt actgataaac gcatagatac ctgcgtcgat
    25261 cgctccggta cgcataataa taccaaaagc acctccaaca acaaggatca atgcagcgat
    25321 ccctacagca gatccatact tatctccaga tactagtcct tcaaaaacaa aatttaagaa
    25381 accaaagccg ccaaaatcat ctgttcccca tattttggct gttttatgca attttttaga
    25441 agtatcatag atattttcgc cgtactgatc gtaaagcaca tcatctgtca aggagatttc
    25501 atctagtttt tcctgcgtaa gatttttttc tccgtcagca atgacttttt ccaatccttt
    25561 ttccggaaca tccagttttt cacgttctgc tggatgatct tgcagttctt ctaattgatc
    25621 aaacacatac gacttatcca gctcgtaagc ataacggaag gaatcttgac gtaaaacagt
    25681 acgagtactt gtttcgccat ttgcatcttt gtattcaata tcttgcgtgc tgaactttcc
    25741 tgctggaacg acaaaagtca gtatccatgc aaagatgact acaccaaaaa taatgacata
    25801 cgtatggggt gtcgataatt tagtgaagtc gcgtttcggt tttcctgaag aatttgtttt
    25861 cttcaaattt atttcttcct ttcttttttc agtaattttg atgaataaaa aatggataat
    25921 caaatgaat
//
LOCUS       DAYYHB010000036        25665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000036 DAYYHB010000000
VERSION     DAYYHB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.037"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..544
                     /locus_tag="ACHA6B_001424"
     CDS             101..544
                     /locus_tag="ACHA6B_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8949007.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="ACHA6B_001425"
     CDS             complement(550..1734)
                     /locus_tag="ACHA6B_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8949008.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="ACHA6B_001426"
     CDS             2002..2700
                     /locus_tag="ACHA6B_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8949009.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="ACHA6B_001427"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="ACHA6B_001427"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8949010.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="ACHA6B_001428"
     CDS             complement(3510..4043)
                     /locus_tag="ACHA6B_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949011.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="ACHA6B_001429"
     CDS             complement(4340..6721)
                     /locus_tag="ACHA6B_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8949012.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACHA6B_001430"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="ACHA6B_001430"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8949013.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="ACHA6B_001431"
     CDS             7422..7961
                     /locus_tag="ACHA6B_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8949014.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="ACHA6B_001432"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="ACHA6B_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8949015.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="ACHA6B_001433"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA6B_001433"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="ACHA6B_001434"
     CDS             9068..10234
                     /locus_tag="ACHA6B_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8949016.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="ACHA6B_001435"
     CDS             10280..11776
                     /locus_tag="ACHA6B_001435"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8949017.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="ACHA6B_001436"
     CDS             11857..12540
                     /locus_tag="ACHA6B_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8949018.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="ACHA6B_001437"
     CDS             13185..14375
                     /locus_tag="ACHA6B_001437"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8949019.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="ACHA6B_001438"
     CDS             14477..15430
                     /locus_tag="ACHA6B_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8949020.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="ACHA6B_001439"
     CDS             15499..16848
                     /locus_tag="ACHA6B_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8949021.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="ACHA6B_001440"
     CDS             complement(17031..17354)
                     /locus_tag="ACHA6B_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949022.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="ACHA6B_001441"
     CDS             17483..18646
                     /locus_tag="ACHA6B_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8949023.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="ACHA6B_001442"
     CDS             18716..20065
                     /locus_tag="ACHA6B_001442"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8949024.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="ACHA6B_001443"
     CDS             complement(20110..20541)
                     /locus_tag="ACHA6B_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949025.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="ACHA6B_001444"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="ACHA6B_001444"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8949026.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="ACHA6B_001445"
     CDS             23771..24367
                     /locus_tag="ACHA6B_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8949027.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="ACHA6B_001446"
     CDS             24489..25637
                     /locus_tag="ACHA6B_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8949028.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT         8536 a         4460 c         5345 g         7324 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaa
//
LOCUS       DAYYHB010000037        24270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000037 DAYYHB010000000
VERSION     DAYYHB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.038"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACHA6B_001447"
     CDS             <1..572
                     /locus_tag="ACHA6B_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8949029.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACHA6B_001448"
     CDS             576..1217
                     /locus_tag="ACHA6B_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949030.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACHA6B_001449"
     CDS             1214..2131
                     /locus_tag="ACHA6B_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8949031.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACHA6B_001450"
     CDS             2128..2799
                     /locus_tag="ACHA6B_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949032.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACHA6B_001451"
     CDS             complement(3340..3828)
                     /locus_tag="ACHA6B_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8949033.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACHA6B_001452"
     CDS             complement(4029..4295)
                     /locus_tag="ACHA6B_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8949034.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACHA6B_001453"
     CDS             complement(4295..4558)
                     /locus_tag="ACHA6B_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8949035.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACHA6B_001454"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACHA6B_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACHA6B_001455"
     CDS             complement(5361..7184)
                     /locus_tag="ACHA6B_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8949036.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="ACHA6B_001456"
     CDS             complement(7781..10486)
                     /locus_tag="ACHA6B_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8949037.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10792..11739)
                     /locus_tag="ACHA6B_001457"
     CDS             complement(10792..11739)
                     /locus_tag="ACHA6B_001457"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8949038.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11929..12198
                     /gene="rpsN"
                     /locus_tag="ACHA6B_001458"
     CDS             11929..12198
                     /gene="rpsN"
                     /locus_tag="ACHA6B_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8949039.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12374..12736)
                     /locus_tag="ACHA6B_001459"
     CDS             complement(12374..12736)
                     /locus_tag="ACHA6B_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8949040.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12747..13868)
                     /gene="alr"
                     /locus_tag="ACHA6B_001460"
     CDS             complement(12747..13868)
                     /gene="alr"
                     /locus_tag="ACHA6B_001460"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8949041.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13884..14234)
                     /gene="acpS"
                     /locus_tag="ACHA6B_001461"
     CDS             complement(13884..14234)
                     /gene="acpS"
                     /locus_tag="ACHA6B_001461"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8949042.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14364..15875)
                     /locus_tag="ACHA6B_001462"
     CDS             complement(14364..15875)
                     /locus_tag="ACHA6B_001462"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8949043.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16197..17549)
                     /gene="murF"
                     /locus_tag="ACHA6B_001463"
     CDS             complement(16197..17549)
                     /gene="murF"
                     /locus_tag="ACHA6B_001463"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8949044.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17702..18778)
                     /locus_tag="ACHA6B_001464"
     CDS             complement(17702..18778)
                     /locus_tag="ACHA6B_001464"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8949045.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18974..20719
                     /locus_tag="ACHA6B_001465"
     CDS             18974..20719
                     /locus_tag="ACHA6B_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8949046.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20999..21436)
                     /locus_tag="ACHA6B_001466"
     CDS             complement(20999..21436)
                     /locus_tag="ACHA6B_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8949047.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21420..22793)
                     /locus_tag="ACHA6B_001467"
     CDS             complement(21420..22793)
                     /locus_tag="ACHA6B_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949048.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23115..23603)
                     /locus_tag="ACHA6B_001468"
     CDS             complement(23115..23603)
                     /locus_tag="ACHA6B_001468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949049.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23625..24227)
                     /locus_tag="ACHA6B_001469"
     CDS             complement(23625..24227)
                     /locus_tag="ACHA6B_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8949050.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5166 c         4487 g         7658 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    10861 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    10921 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    10981 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    11041 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    11101 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    11161 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    11221 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    11281 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    11341 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    11401 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    11461 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    11521 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    11581 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    11641 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    11701 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    11761 ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    11821 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    11881 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    11941 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    12001 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    12061 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    12121 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    12181 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    12241 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    12301 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    12361 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    12421 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    12481 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    12541 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    12601 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    12661 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    12721 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    12781 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    12841 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    12901 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    12961 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    13021 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    13081 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    13141 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    13201 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    13261 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    13321 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    13381 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    13441 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    13501 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    13561 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    13621 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    13681 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    13741 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    13801 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    13861 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    13921 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    13981 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    14041 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    14101 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    14161 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    14221 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    14281 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    14341 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    14401 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    14461 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    14521 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    14581 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    14641 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    14701 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    14761 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    14821 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    14881 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    14941 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    15001 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    15061 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    15121 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    15181 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    15241 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    15301 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    15361 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    15421 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    15481 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    15541 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    15601 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    15661 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    15721 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    15781 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    15841 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    15901 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    15961 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    16021 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    16081 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    16141 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    16201 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    16261 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
    16321 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    16381 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    16441 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    16501 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    16561 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    16621 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    16681 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    16741 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    16801 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    16861 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    16921 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    16981 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    17041 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    17101 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    17161 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    17221 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    17281 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    17341 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    17401 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    17461 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    17521 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    17581 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    17641 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    17701 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    17761 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    17821 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    17881 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    17941 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    18001 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    18061 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    18121 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    18181 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    18241 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    18301 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    18361 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    18421 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    18481 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    18541 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    18601 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    18661 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    18721 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    18781 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    18841 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    18901 tttatttttt gtcggctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    18961 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    19021 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    19081 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    19141 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    19201 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    19261 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    19321 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    19381 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    19441 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    19501 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    19561 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    19621 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    19681 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    19741 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    19801 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    19861 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    19921 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    19981 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    20041 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    20101 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    20161 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    20221 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    20281 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    20341 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    20401 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    20461 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    20521 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    20581 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    20641 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    20701 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    20761 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    20821 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    20881 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    20941 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    21001 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    21061 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    21121 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    21181 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    21241 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    21301 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    21361 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    21421 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    21481 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    21541 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    21601 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    21661 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    21721 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    21781 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    21841 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    21901 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    21961 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    22021 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    22081 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    22141 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    22201 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    22261 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    22321 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    22381 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    22441 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    22501 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    22561 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    22621 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    22681 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    22741 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    22801 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    22861 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    22921 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    22981 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    23041 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    23101 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    23161 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    23221 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    23281 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    23341 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    23401 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    23461 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    23521 ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    23581 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    23641 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag
    23701 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    23761 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    23821 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    23881 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    23941 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    24001 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    24061 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    24121 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    24181 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    24241 atcttttttc atttattaaa actgcctcct
//
LOCUS       DAYYHB010000038        23917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000038 DAYYHB010000000
VERSION     DAYYHB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.039"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..2752
                     /gene="clpB"
                     /locus_tag="ACHA6B_001470"
     CDS             143..2752
                     /gene="clpB"
                     /locus_tag="ACHA6B_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8949051.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2903..3589)
                     /locus_tag="ACHA6B_001471"
     CDS             complement(2903..3589)
                     /locus_tag="ACHA6B_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949052.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3567..4061)
                     /locus_tag="ACHA6B_001472"
     CDS             complement(3567..4061)
                     /locus_tag="ACHA6B_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8949053.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4081..5301)
                     /locus_tag="ACHA6B_001473"
     CDS             complement(4081..5301)
                     /locus_tag="ACHA6B_001473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949054.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5448..7283
                     /gene="lepA"
                     /locus_tag="ACHA6B_001474"
     CDS             5448..7283
                     /gene="lepA"
                     /locus_tag="ACHA6B_001474"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8949055.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7479..8390
                     /locus_tag="ACHA6B_001475"
     CDS             7479..8390
                     /locus_tag="ACHA6B_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949056.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9401..9637
                     /locus_tag="ACHA6B_001476"
     CDS             9401..9637
                     /locus_tag="ACHA6B_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949057.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9750..9992
                     /locus_tag="ACHA6B_001477"
                     /pseudo
     CDS             9750..9992
                     /locus_tag="ACHA6B_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10082..10321
                     /locus_tag="ACHA6B_001478"
     CDS             10082..10321
                     /locus_tag="ACHA6B_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949058.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10468..14751
                     /locus_tag="ACHA6B_001479"
     CDS             10468..14751
                     /locus_tag="ACHA6B_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8949059.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14850..15215
                     /locus_tag="ACHA6B_001480"
     CDS             14850..15215
                     /locus_tag="ACHA6B_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949060.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15217..15669
                     /locus_tag="ACHA6B_001481"
     CDS             15217..15669
                     /locus_tag="ACHA6B_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949061.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16118..17593
                     /locus_tag="ACHA6B_001482"
     CDS             16118..17593
                     /locus_tag="ACHA6B_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8949062.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17616..17927
                     /locus_tag="ACHA6B_001483"
     CDS             17616..17927
                     /locus_tag="ACHA6B_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949063.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18288..19544
                     /locus_tag="ACHA6B_001484"
     CDS             18288..19544
                     /locus_tag="ACHA6B_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8949064.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19541..20086
                     /locus_tag="ACHA6B_001485"
     CDS             19541..20086
                     /locus_tag="ACHA6B_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949065.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20087..20587
                     /locus_tag="ACHA6B_001486"
     CDS             20087..20587
                     /locus_tag="ACHA6B_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949066.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20946..21224
                     /locus_tag="ACHA6B_001487"
     CDS             20946..21224
                     /locus_tag="ACHA6B_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949067.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21244..21588
                     /locus_tag="ACHA6B_001488"
     CDS             21244..21588
                     /locus_tag="ACHA6B_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949068.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21607..22518
                     /locus_tag="ACHA6B_001489"
     CDS             21607..22518
                     /locus_tag="ACHA6B_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8949069.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22597..23589
                     /locus_tag="ACHA6B_001490"
     CDS             22597..23589
                     /locus_tag="ACHA6B_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8949070.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23586..23804
                     /locus_tag="ACHA6B_001491"
     CDS             23586..23804
                     /locus_tag="ACHA6B_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8949071.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
BASE COUNT         8265 a         3795 c         5032 g         6825 t
ORIGIN      
        1 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ctatgtgtat attttgacca
       61 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
      121 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
      181 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
      241 tgtatggaaa atctttttac aatctaatca ttttggtcga aatttctata cggatgccgg
      301 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
      361 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
      421 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
      481 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
      541 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
      601 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca
      661 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
      721 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
      781 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
      841 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
      901 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
      961 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     1021 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     1081 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     1141 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     1201 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     1261 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     1321 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     1381 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     1441 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     1501 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     1561 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     1621 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     1681 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     1741 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     1801 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     1861 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     1921 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     1981 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     2041 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     2101 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     2161 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     2221 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     2281 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     2341 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     2401 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     2461 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     2521 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     2581 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     2641 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     2701 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     2761 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     2821 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     2881 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     2941 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     3001 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     3061 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     3121 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     3181 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     3241 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     3301 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     3361 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     3421 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     3481 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     3541 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     3601 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     3661 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     3721 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     3781 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     3841 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     3901 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     3961 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     4021 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     4081 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     4141 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     4201 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     4261 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     4321 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
     4381 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
     4441 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
     4501 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
     4561 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
     4621 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
     4681 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
     4741 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
     4801 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
     4861 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
     4921 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
     4981 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
     5041 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
     5101 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
     5161 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
     5221 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
     5281 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
     5341 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
     5401 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
     5461 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
     5521 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
     5581 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
     5641 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
     5701 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
     5761 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
     5821 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
     5881 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
     5941 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
     6001 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
     6061 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
     6121 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
     6181 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
     6241 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
     6301 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
     6361 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
     6421 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
     6481 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
     6541 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
     6601 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
     6661 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
     6721 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
     6781 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
     6841 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
     6901 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
     6961 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
     7021 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
     7081 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
     7141 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
     7201 tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
     7261 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa ataaaccttt
     7321 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
     7381 tcacggcgat actaaaatta acaaaattgg aatatttttc ttgaagattt ttagctgtca
     7441 gtatatgatg aaagcatctc ataggaggtg cttttttcat ggttgataat tcaagtggaa
     7501 gggttcttaa atctctaaga aaagagaaaa aactatctca aaaaaagtta gctgatttag
     7561 caggtatttc acaaagtact ttagttaaat atgaaaaagg gtcaagaaaa atccctaaag
     7621 acgtagataa tactctaagc aagattctga atatagaaac tctaataaaa gatgaggaag
     7681 ataagattga gattctcatc ggtaaactaa ttgcatacag agatatgaat aagttactta
     7741 acaaagagtt agctgataat ataggaatta gtgaagtatt actcagttat gtgttaaata
     7801 gaaagagaaa tccgtcaaaa gaaatgcaaa aaaaaatcga tatttttcta ttaagtaatg
     7861 aaaaagaaat tttgaaggag attaatcgtg atagtgagat atttagttta tcaaaagacg
     7921 ataaaattgt tatgggaaaa agaatacgag aagtccgaaa gaaccgggaa gagacgcttg
     7981 aaaaatttgg taaaaacttt accatctata caggcaaaaa tgttataagt agatgggaaa
     8041 aaggtattaa tattccagat attgaaaaac taatgaatat tgcctatctt ggtaaggtca
     8101 ctgttcctta ccttatgtac ggagaagatt ataagaatat acttccaaaa gatgaaagag
     8161 ttagcgattt taaaaagata aattctttta gtatgggatt aaggatgagg aaaattagga
     8221 aagattatta tctagaaagg gaggagtttg gaaagctttt ttctccttca atttcaaaat
     8281 ggtccataga taggtatgag aatggtagag atatacccaa tactaatcga attatccaat
     8341 atgcttatat aggaaatctt tctttagaat ttttgattta cgggatataa aaatagttta
     8401 cgtcgatact tattaagtta tcgacgtttt ttattataga aaaaatttat aaaaggaggt
     8461 ttcttgatat gataagaaac aaaaagggtt tgttgaaagt tgctttactg gcagcttttt
     8521 tctaagtaac tttttgacaa gctcaatggc aacacaagcg gaagaagtaa tacaagaact
     8581 aactgttatg aataacagct agataaaatg ggctatgtat gttatccaac aatttagtaa
     8641 tcactagaag cctagcgaaa gttcgcttga taagttatag atgagtattg atttgtagtg
     8701 tttccaaatc aaaagggtac agagccacat gaaatatgtt gtcctctgca gcgtcaggat
     8761 gggaatccat accatggaga ttcggagatc tgtgagggaa tacttacgtg gttaatatgt
     8821 cagttgtgtt ggacgtagta atggtcatag tgggtagatt gactgaattc ttatatgaga
     8881 aaatgaaacc aacaagaaga tagggttatt tatacagaat tggggatctt ttctggaaaa
     8941 gtaatcggat aatgcgagat tcgtacggtc ttttttaaat ttctctaaaa aaaacggcat
     9001 cgaatgggtt gggtgattga taagcaatga atcatagact gatatagaat aaaaaatgat
     9061 tctatttatc tttttacatc tatcgttttt tcaagtagcc gcaaattata tcagcattca
     9121 acaatttttg tatacaagtt ctagtaaaag gatctgaaga aaaagttggt tgttgtgaag
     9181 ataaaaaaag aatcaggttt tactgagttt ttcttcaaaa tttgtgttat taggttagga
     9241 caaagataag aagaagtatg aagatttaaa aaaggaatga ggaaaagata gtaatttccg
     9301 attattggag tttttttatt tataaagaag aagcgaataa gttgtgattc ctagatttcg
     9361 cgcgtgatta attaatgagc aaaaaatgcg gaaagtttta gtgtttaata cacgaggaca
     9421 aagtttctta ggtattgaag gcacacgtgt aacggttgat gggtggtgtg attgtttcga
     9481 ggttggaaaa catgttgaac ttatggcatt tactgagcta aaagataaga acaacgtgga
     9541 atttttcgag agtgatattg gttgggacga tcatgaagaa gtttatggaa aatttatttt
     9601 gcggaaggga aattcttata cgaatgggga agcataagta gcgatttgtt agagatcatt
     9661 gataggattg aaattgtcgg gaatatttat gagaatctag agttatttga ggtgatagaa
     9721 tgagtgatgg ttttgaacaa gtatcaataa ttaaaaatca agttcgggag attcttagaa
     9781 aaaaaggcta tttggtagat agttattttg aaggagatta cgaaacatgg gtaggtgtct
     9841 atgctcgtcc agagaataaa cctacctatt tagatcctac gacgtcagaa gatgcctatt
     9901 tacaaaatag gtatcgagta gatggtttta agcaagactt tgcggagtgg tttgaatggg
     9961 aaattgaaaa cggagaagta aaagaagaat aagagtactt gttgtatcaa aataagccag
    10021 tagaacaacc aaaccagtag ctagtaatga caataatact gaggaatcgg atcattatgg
    10081 tatgattgat atttctcttc cagaagatct taagccagat cctgtaacaa ttggcaatag
    10141 tgtcatgatt tttgattcat tagatgaagc tatgtcatat ggttggaaag aaattaagga
    10201 tgaaggtagt aattggtatg gcatgagatt tggtgatggt gaagttgcca aaaaagctgg
    10261 tattccaatt gggaaatgga cagttgattt ccacgaagca ggttcttttt ttgttctgta
    10321 attatagtgg gagggtaaaa tcattgatga aatgtgttca gataagagcg ccagataaaa
    10381 atagaagaga cagttcacgt cgttatcact tttggtaacg gcgttttgtt ttattatata
    10441 aaattctcag gaaaaggagg aattcttgtg tatgcaaaaa cgaaaatgaa ttggttgaaa
    10501 gcactcgcat tggtggcttt ttctcaattc tgtttcttgg tgggaactgc tcaagcacat
    10561 gcagaagaaa cgacggctat acaacctacg atggaagcca ttgcccaagt cgcttctgaa
    10621 gtgacagaag tccaaccagc agaagccggg gtcccagctg gtgaagccac tgttgaacag
    10681 gcccctgtga cagaagcgcc aacggtcaat tccaaaacga ctctcgatca gacgattggg
    10741 gaaacacaac acccagggaa tagtcctggt acttcacaac cagttgaaga aactgcctca
    10801 attacaaccc ctgcttcaga aacgtccact ctagaaggag aaaaagctgc cgaagcaacg
    10861 ccttctgtgc agccagagtc ttctgagaaa caagcggaag aaaagaaacg aatagaagaa
    10921 acagtggaac aaccagcggc tactgctgac aaagaaggaa aaaatgagga aaacgttgaa
    10981 aaaacaatct cttcaaccgc agctagtcaa aatcaaccaa cgaaaccaaa caaagaatct
    11041 acggctagta ttgaaaatca aaagaaaaaa gagcagaaca ttccaaaaac aggtacacct
    11101 acactgttag taacagtacc aaccaatcgt tcggttcgtg ttcgccgtgc aaccaatgca
    11161 gctgttttag gtggtcggaa aattgattgg ggcgaaaata ttggtgttac ttctagtccg
    11221 aacaatctaa acttcacttt aaattatatg gttcgttttg agattaatgg acaagtggcg
    11281 ttttgtattg agcctggtgt ctcagccgca gaaggtcaag tttatacagc tactgctttg
    11341 aaaaactatt tgaaaaattc aaatgtacgg aaaaaaacaa gcttgatctc ttatttagga
    11401 tatgtcacaa ataaagacaa atccgatgaa caatacattg cggcacaatt tatgatttgg
    11461 gaagctttgg gcacaaaaat cccaatcaaa aatatccgtg ttgattatga tcgtcgaaaa
    11521 gcagaaattc aacgtttgat taataacttt ggtgaaaaag caacttttaa taatcaaaca
    11581 catacggtaa aaattggcga aacgttgaaa gtaaagaata aaaatgattt tttgaaatat
    11641 gtgaaagaca ttcaaaaacc aaaaggtgtc aatgtatcta ttcacggcaa tgaagtatgg
    11701 attacagtca caaaagaggc accggataaa gcaaaaattc aattgaataa aattaaagtt
    11761 caaggtgcgc cattagccta tcgtcgtcca ggagctcaaa caattggtgt gttacatcca
    11821 acagaaccta atgcgtctgt tattaattta caaattctaa aacaaggaaa cattcgtgtg
    11881 tttaaagaag atgcggaaac tggtgtgaaa gcacaaggtg cagcttcttt agaaaatgcg
    11941 gtctatggtt tgtatcaagc agatgggaaa aagattaaag aaattacctt gaaaaaaatt
    12001 aacggcaaag tacaggcaga aattaaagat cttgatcctg gcaaatatgt tttaaaagaa
    12061 atcaaagcgc caaaaggtta tgtgctttct gatgaagcaa ttgaaattgt tgttgaacct
    12121 ggaaaaactg tcaatgcgac cgcaaaagat caagtgatca aaggaaccat tgatttagtg
    12181 aaagttgcta ataaagattt agtcgattca acgaatccag ataacaaacc aaaattagct
    12241 ggcattgaaa tcactttgac gtcaaaaaca actggcaaag tggtgaaaac cgttgtgaca
    12301 gacaatgatg gttatgctac ttttggtaaa aataccgttg tctttgatac gtacattctt
    12361 tctgaaacca aaggaaaaga agggtataaa ctatttgaac cacttgaagt cacaatttct
    12421 gaaaatgggc aaactttcca ttatgtttta gaagataaag taattgaaca acgtcttaaa
    12481 gtggtgaaag tggatcaaga aacaggaaaa gtgattccac ttgctaatac agaattcaag
    12541 atttttgata ctttagccaa tcgttatgtc acaatggaga ttccaaatga tacagaaaca
    12601 acggatatct ttaaaaccaa tgaaaaaggc tttttcctaa ctaacggtac acttacttat
    12661 ggaaaagatc gctatcgttt ggaagaaatc aaagcccccg taaattacgt tttgaatcaa
    12721 aacccattag tgttctcgat taccaatgat acagaaccga tcaaagtgat ccattttgcg
    12781 aaccgtcaag cacggaaaag tgtaaaatta ttcaagtatg aagaatggaa caagaagaaa
    12841 acgccgctcg caggtgtgaa ctttaccttg tataacggta aaaatcaagc aattggtgaa
    12901 tataccactg atcaaaacgg ggaaatttta gtgaaaggtc tagttgccga tgactattac
    12961 ttcttggaaa atgccccatt agaaagtttc caaccaaata cagataaaca agcattcacg
    13021 gttgcattca aacaaaatgc gccagatgat ggggaaacgt tagtggtttc tgtacgtaat
    13081 tacttgattc caccaaaact agaaacacaa gcgtcaaata aagccgatgg tgaaaaagtg
    13141 gttgatccat tagaaacgat cgtcattaaa gacagtgttt cttataccaa cttgtttgtc
    13201 ggaaaagaat acaccatttc aggagtattg atgaacaagg caacgaatgc gccaatttta
    13261 caagatggca aagaaatcac aagtaccttg gcctttattg cagacaaagt caatggcaca
    13321 aaagaccttg aatttgtggt taatgctagt gttctgaaag gtcaaacgat cgttgttttt
    13381 gaaaaacttt tccgtgatgg cattgaagtt gcagcacatg cggatatcaa cgatgaagcc
    13441 caaagtgtga aagtgaataa tccaaccgtt cataccacgg ctgtctttga agacggattg
    13501 acagtcgcag acccaacagg attgtttacc ttaaacgata ccgttgaaat taccgagatt
    13561 cttccaggaa aagcatactt ggtcaaaggg atcttaatgg ataaagcaac cggtgaaccc
    13621 ttgcttgtgc atggaaatac ggtagaaacg agtgttggct ttattgccaa agcagaaaat
    13681 gaaacggtga tcgtaccatt tacctttgat ttgacaggta taaatggtcg tgaaatcgtc
    13741 gtgtttgaaa gtctttttag aaatgatgaa gaactagcaa accataaaga catcaacgac
    13801 aaagggcaaa cggtacgagt aactaaccca agtgtacgta caacggctac acataaaccc
    13861 actggcttga aagaggttaa tccattaacg aaagtcacca ttacagacgc tgtgaaatac
    13921 cacgatttgg tcgttggaaa aacatacaca gtcaacggtg ttctaatgga caaagtaacg
    13981 gaaaaaccag tgctagtaga tggaaaagaa gtcacaagta ccttaacgtt tgtggcagaa
    14041 acaacggatg gtacagtcaa tctagacttt attctagacg cacgtgcgtt gcgaggcaaa
    14101 gaagtcgtgg tctttgaaga tctgtttaga gaaggcgtat tattggcttc tcacgcagat
    14161 attaccgaca atgaccaaac catcaaaatc agtaatccaa ccattacgac gaaagcgacg
    14221 aataaaaatg gcggaaaaga gatcttggcc ttgccaaatc aaactgtgct tgaatgggtc
    14281 aaagtagacg gtcttgcgat tggtcaaatc tatcgcttaa ttgttcaagg ctacttaaca
    14341 ccagacggca caccatttga aggcactcat gcggaaaaag tctttgcggc cgataaagaa
    14401 atgatggaat tcttatttga attcttagtg gacggtcgca atcttgcagg aaaaggcttg
    14461 agttttgcgg aatggctcca agcgaaaacg gaagatgaga aagaaacgtt tgaagaagtg
    14521 gcgaaacaca atgtagatct aacaaacaaa gatcaaacgg ttcaaattgt ggaagcgcca
    14581 aaaccaccgc aaccagtgaa accagaacaa cgggttaaac cagctactgt tgtaccaatg
    14641 ttgccaaaaa caggtgaaca aacaagtagt atacttatgg ttcttggatt agtagtatta
    14701 gtcattgctt taggtataac actttataaa gtacgtaaag ccaaagatta gtcatgagga
    14761 acaagcggtt aggattttta accgcttgtt tgattgtaat gtagaaatat ttagaattta
    14821 ttttggtttt taagaggagg aaaaacttta tggcagaaaa agcattgcgt atgaagaaag
    14881 agtttttagg tcaagtattt ttattgggtt cacgggaacc taatccacca aaggaaatta
    14941 atggtgtgtc tgggcgatat gttcaattac aagcagagtg ttcgcgagaa gaacagcctg
    15001 ctttttttgt ttatctcaca gctgaagagg ataaaagatt acgattacca gaaaaagagg
    15061 tgcgtatttc atttgaagaa gcttattttg tttttcgtaa agaacgagtg aatagtcgtg
    15121 aaacaaatga tatgggacaa acagagcgtc gaacaactgg ctatcaagat gttggtgtct
    15181 ttttaactgg tgtaaaaatt ggggaggttt tgtaagatgg gattaaaatt tccaaataat
    15241 tatgacatta cagaagcatt agatgaaact tgtttaggag ccgtacgctt tggcgcatta
    15301 gaagagtctg atcgtgtatt tgataatgaa acaggcgatt atacgggaga aattcgatca
    15361 cagcgagcac aattagtttt tggaaatcca gatgaaccaa agattgcacg acgagttaaa
    15421 tttccagcag atgtagattt gactggtttt aaacgacgtg atcctgtgaa attaattgat
    15481 ccagaatatc atgtggtata tattccagca caagatggca atcgtgaata ttcagattta
    15541 gtgatttatg cgaaaggatt agagaaagta gaagctccaa caaataatcc taatatgaaa
    15601 caatcacaag aaaaaggttt agatcaaaaa caaccaagtg ttgattctaa ggaaaaagag
    15661 aaaaaataag gtttttttga aagaatagga aggagtcttc ctattctttt tatctaagat
    15721 agaaggaagg ggagattttg aattcagata aaaccaatca aatactttga gagattgtgc
    15781 ttgttatcaa aatatccaac aattgattga gtattttgga gacaatccaa cgatttttgt
    15841 acaaacaaat gcatatattt gtgcggttat tgggcagtgt atgtttttac gtgatcttgt
    15901 tcgtttttca ttaccgtacc agtggaaaca agatcatcag gaagtgattt ggattcgtat
    15961 agtacgaata tgtaatatgt gtgcacaccc ttataaaaat cgattaaatc tttttcgatt
    16021 atgttttttt actaaatgtg atattcctgt attagttgct ttttgtcaaa agtttttagc
    16081 acaggaaaag taaaaacaga aaggaggaat gtgacaattg aaagctcatt ttttcaagta
    16141 tcgtggtcgg cgaattcgct atttcaatcg ttatttatgg ttttgctatt atggtttctt
    16201 tttgtttcca tttttgattc tgttaggttg gtttagttgg aaagtatttt atccacgttt
    16261 aagtacgttt tcgaattggc aaatgtatct tattcctgga ataagtattg tgttgtattt
    16321 gttgctggtt agtgggttaa ttttagggtt gatgcattgg tcgcgactaa aagaaggata
    16381 ttttgcctct gtttattggc gtcagttact tgttcgaatg ttaattgaca atcggtttgt
    16441 ttatacaaag aaacaagtta gtgagaaaca aacaagggaa aaaatgagat taccaaaagt
    16501 ttatttttat caaaagaaac aagaattagt ggtttctttt cctttagatg gtgggcgttt
    16561 tcacgaacga tttttaaaaa tgggtcattt gttatctgaa gtatttttgc gtgatttaat
    16621 tcgtgaagaa cgagaaaaag cccgaatcaa ttatgtattc ctttcagatt ctggtcggat
    16681 tcctattgat caatgtatag cagaaaatgg acgtattcat ttaatgaaaa ctttggattg
    16741 ggtatatgat caatcaccaa atatgttgat tgctggtgca atcggtggtg ggaaaaccta
    16801 tttattgtat tctttaattc aagcttgttt aagtgttggg acagtagata tttgcgatgg
    16861 aaaagcggca gatttagcag cattaggtga cgtcgatgta ttcaaaggac atgtgtttta
    16921 caaaataaat gaagaaatga ttatttgttt acgaaatgca ttaaaagaaa tgaatcaacg
    16981 gtatgcctat atgaaaacca tgaaagatcc taaatatgaa ccagggaaaa attatgctta
    17041 ttatgggtta ccaccgcatt ttatcttttt tgatgaatgg gcagcttttt atggttcgtt
    17101 ggatatggaa gcgagaaata gagtcgataa attgattcag ccattagtat taaagggtcg
    17161 tcaagcaggg atgtacatta ttttagcaat gcaacgacca gatgcagaat attttccaag
    17221 tggtgttcgg gataatttaa cattccgtat ttctgttggt cgattgtctc ccattggtta
    17281 tttaatgact tttggagaag attttaaaaa taagccattt tacaacaaag atcaacctgg
    17341 tcgtggatat gcttttccag gatttgatgt acctgtagag ttttttgcgc catttgttta
    17401 caaagattat cactttatca aagaatttga gaaatttact gaaatgattg tctatgattt
    17461 tacagtaaaa gaggatagtc aagatcaaac gatgggagat gataatcagt tgttacaaga
    17521 acaactagag cgccaaatgt atttagcaaa agatccaaaa ggaacaagtt tttctgatgc
    17581 gcatattttg taatcaacta aggaggtgaa gttaaatggc agagagttgt tattatagag
    17641 tagctacagc aatacgaatg attaatcctt ctctttcttc cagaacgttt tatgattggt
    17701 tgaatcgtat tgaacaagta actgattacc gctttttgcg caaagaacgt gtttttacag
    17761 gaaaagttat taatcaagta ttgctaacga aaaaagatat agaacgattg actcgattgt
    17821 atcattatcg agtagattta gaagaggatt taactttgtc aatttatcgc gttttttctc
    17881 ctgaaaaata tagtgaaata acaaaattag atcatttaat tttgtagtta ttatctatcg
    17941 ggagggctct agacgtagtc tggtagccgc actctgcctg gcggtgcgca ttaaggctgc
    18001 gaagcatggg cctataatgc gcactcgcta gcagcgtgtg cagctcacaa agaggtcgtg
    18061 cgcttgcgca cggcctcttt gtgtaaagcc ccccgatata taatatgggg ggcagaaata
    18121 acataacaca tagaaaaaag ttttataaac ccttttgtat caagtgatta tctaaggtca
    18181 gcagttgtac tacttttccc aaaaaacgag ggggactagt tgatatatcg gtgtttctaa
    18241 agggttaaaa aaaatcgatt ttgttatttt ataaaaggag cgagaaaatg acaccgattg
    18301 agctaaaaag ctacagaaag aagttaagtg caattcataa tataagtgga gataaaaagc
    18361 cgctatcaca agcaagattt gcgcaattga tgggctgctc aaaagattac attagaaaga
    18421 tggaacaagg gaaaagaaaa atcccagagg attttgaaca acgaatcaag gaacgctttc
    18481 gattgtatat gaaaagcgga atagatcttc gagcaatgat tgattatttg cgtctttcct
    18541 tttttgggac agactacaaa acagtgattc aacgtgtatt acgaatggat ctgacagaat
    18601 ttgaaacacg tccaacgggc ttgtatcttt ttgactgtct ttctgttcgt gggaatatct
    18661 gggtgttctg gcattcaaaa cgaactgtaa cagaaaatgt acttgtgcaa ttttcaggac
    18721 aaggttgccg ggaatatgag gaagtcatgc gtgaacaaca aacagattgg cgaacatggc
    18781 tatattccat ttggaaaaat caaggaaagt tggaagctgg ttatacacgt gtgcaatgta
    18841 gccgattcga tattgcgtta gatgaacttt ggggattgga aacggaagaa catttcgatc
    18901 tctttgaatt actagaaaaa caaaaagcag gtttattaga aatggacttt aagagattcc
    18961 agaatattgg cggttgtttt ttagatgatg gctattttcg aagtgaagga ttatctttgt
    19021 attttggatc gcgaaagagt cctctctttt ttaattttta tgagaaacgt tttgaaattg
    19081 cgaaccgaga aaaaatttca gaattagaag cattaacgaa atatggtatt tataatcgat
    19141 atgagttacg gttagccaat gagaaagcaa cacaagcagt agaagcattt attttaggtg
    19201 attcaccgaa acgtttagga gagattggcg ttggtttaat taatgcaaaa cttattgttt
    19261 gggatagact ggaaagtgat gcacggattg ttgataaacg atgggcaaaa ttatttcgtt
    19321 cgatggaaga attggatttt acagtaaagc cagaaaaatt ctcattagat cgttcagaac
    19381 gctggttttt gaaacaagta gctccgacat taaagttgtt acaagaagca gatcgtattt
    19441 taggaagaga ctttgtacaa gaagggattg ataatgccga actaaatgac gagcaacaaa
    19501 actatctaga atggatggga acgtcaggag ggaaaattgc atgaatgcat atacgacaac
    19561 agaatggctg cgcctgttgg atttaaaggc tgcaatcgaa gcattaaatg aaaagatggt
    19621 tgatttgtct tattttcgtt ttcgagttcc ttatattgaa caagcggtaa aagctggacg
    19681 ttatcaggaa aaagagaact ggcaagaaat tgcacgctta ttagaagttc gtaagggata
    19741 cgaacaagaa ctcgaagagt tggaatttag ccgaaggaaa ggaaggctag aattcattcg
    19801 cttttatcga ttttcattgc caatacctgc gattttagcg gttaaaaaag gctgcgataa
    19861 aatgaagata tatgaaaact gtgtggcagc acttagcagc gaaaaacctc ttatggaaga
    19921 aatttcattg gtgactgtca cttgggaaat gagtcgccag ccaacagaag aacagacata
    19981 tctttcttta gaagagattg agatagaatt agaggaaatt ggtcgctatg ccacttgttc
    20041 tacctattgt ggatcggtca ttagtattgc tggtgtgatt gtctaaatga aaaaaattat
    20101 gatttatgtt ggtgcgtatt ggtcacgtga tccaacagta ttagaaaatc cagaggcggt
    20161 acactattgc ttgcgccaat tattctatct gtataaagaa cgattagaat cgttgattcg
    20221 tcagttacca tatactgaca gacgtttaga tgaacttctt ctaaggtatc cagcaatgta
    20281 taaacgacga aaaaatagat tgttaccaga agagtatccc attgaaaaac atgaacttga
    20341 agggcgattt gtggcttatt tctatgatga tgttcgtatg cgcttagtag aacaacgaat
    20401 ggaaatagaa aatgatcgct actattttat caattattgt aagaagcgta agtatcaagt
    20461 aactgatgat ttttatgatt gtattttaca agatggggaa gtgattctat ccaaaattgc
    20521 tccaagtttt cgtgaacttg tgccgattga ttttttgaaa aaatgtcatt tgcggattct
    20581 tccgtaaaaa agagctataa aaggaaagaa aagtagaagc caaatcggtt tctgcttttt
    20641 tatttttttt aggaaggaga agatggtatg tcactggaat ttttaggaga gccaattgaa
    20701 aaggaagagt tcgtttcgca ttatatggat ttgtttattc aggtgataaa aaactttgtg
    20761 cgattgatga attttatcca cgcgaacaag actatctagc cgctgaacga ataaaaatca
    20821 gtttattatg ggaaaaattc acagatgaaa ataaccgacc gccagattat cgctatttat
    20881 cagattgttt aacgaataat gtgctttcta aagaatacct atttcacaaa cgtgagaaaa
    20941 atcaaatgtc ggttgaacat ttttgtgatt gttatattgc attattgcgt agtcaacagt
    21001 tattaacagt agaacgatat ggtgcagagt ttggttatgt catgcaagct gagcgtcata
    21061 gagctacaag atgctatgaa aaagaagatc aagtgattga tggctatgaa gaattatgta
    21121 ttcgaaacaa tcgctatttg caacaacgtt tatatgaaca attgatgatg gatttttcaa
    21181 atctctatag agggtatgaa tgttcgatga ttcaagaaag gtagaagaaa agggggaatt
    21241 tttatgtggc gattattatt tttggtggta tgtggtatgg cattgttcta tgtgtatcgt
    21301 gtctatctct atccacgaat ggaacgatgg acaagttggt atagagatcc tttaaaacaa
    21361 cgaaaaggat tgaagcggta tcgttatgaa ttacttgttg aattttcgga tgggcgacaa
    21421 gcacagtatt atttttttgc aaactatcca aaatacaaca ccagtgaact attgattcaa
    21481 ttattcaaaa ataatccata tgttgggtta gaagaaaacg gacgggagtt cttttatcgt
    21541 tcagataata ttagtgcggc gtctatgaac aaaacacgaa ttgcataaaa aattggagga
    21601 ataatcatga aaaaaacaac attagcagtc gtaacaacac taggaatttc attattttcg
    21661 tttcaattag ggagttcatt ggtttatgca gatgaaactg tagaaacacc taagtcagaa
    21721 attgtagaaa cattgccagg ggaaggatta ccagatgttg agacagagct aaatggagga
    21781 acaacggaaa aaccagcaga acctgaacaa ttgcagtcag agaaacctga aaaagagcca
    21841 gtgacaccag aaccagagtt tcctgaatca actttaccga ctgattcgat cgaagaagaa
    21901 ggtgtgcata gttcaacaac aacggattca agtatagtca caactccgga acaatcagga
    21961 acaaccagtt cttctgaaaa gaatactgat tcgtcaagta cggaacagcc aacgatgtcg
    22021 tctaccagtg aagaacaaaa agaagaaccg aaacaacctg ttgaaaaatc agaagcaatt
    22081 ccaaaagaaa ataaacctat tgttccagca aaagaagtca cagtatcggt aaccccaagc
    22141 ggtgaaatta cgacgaatac ttctcaagga atgagtgtac cgattgtcac tagtaatgta
    22201 gaggaattga cccatattcc aacaccaacc accccattaa aagtagcagg tgatcaaaca
    22261 attgttggtg taaaagatgg tattccatta atccaagata gtgaagggaa tttggtaaag
    22321 gatttgtcga ttcctgtaaa gaaacttcca agtggcaata ttgaaatcaa aacagcagat
    22381 ggaaaaacaa aagtcttacc aaagactggt gaagaaatac atgttgcact ttctgttcta
    22441 ggaggagtgt tgacaagtat tgctggattt gttggatata aaagaaagag aaaaggaata
    22501 gaaaatgagg gtaattgaat ggactatttt tcagaaaaac aagcttatcg ttgtcaatcg
    22561 tacgtactag taaaaacata ctagaaggag tagcttatga aaaaacgaca actacggcgg
    22621 aaagaaaaag tttatacaga gaaagtaccg aagcaaaaga aaatccattc aaaaaaagca
    22681 gtgaaatgga tttggtgttt ggttttattt ctttgttttt cagggggctt ggcatttttt
    22741 caatccatga aaacgaaggt tcaagtgtcg tttttgaaag atgaaatcca acaattacaa
    22801 aagaaagaag tacaaagtcg gtcagaatta acgtacacac ctgaaattga atcctttatg
    22861 aatcgttttg ttgctttgta tatgaataca ttcgataatc atgaacaaga acaacaacgt
    22921 caagaaacat taaaaaaaga gttttatgca caaaacttta ttcaagaacc aggaagtgtt
    22981 tcaggtgttc gaaaattaac tgctgcttcg tttattggat taaaagaaat tgaaaccatt
    23041 ccaactgcta gttataaagt aacttatgat attcgctcga tgaatccaga gagtaagaaa
    23101 gaaacaagtg tgacaatcac acaagtactc aatcttccct tttctatttc aaacaagggc
    23161 tgtcgtatta ttgcttatcc ttattttacg gcactaccaa agaatacaga aaaacaacga
    23221 atgattactt atgatgacac attatacgaa acggttgatg cgtcggaagc gaaagatgtt
    23281 gaaggatttg tggaagattt cttagataag tatgcgaaaa acaatgcaaa agatatgtca
    23341 tacatgatga aagaacctga aggactcaat caaacagcta aagttgatca agtagagatg
    23401 aaagtattgc gttataaaga aacgaaaaaa ccttttattg taaaagcaac ggtcgtcttt
    23461 cgagatggtg atacgtcact tttacaaaaa gaacaaatga cgattgtcat tacgaaaaaa
    23521 acaaatcagt attatgttga aaaattaaca cataattggt tggataggaa aggagaagaa
    23581 cggaaatgac actgaaggga attgtagatt ttatattaac gcaaggtggc tattttttaa
    23641 taatggtggt tgtttatctg ggattgcgaa attttatgaa atcaaaagta ggaaatgcat
    23701 taatatctat cgcagcaggt gccgcagttt atttcttctt aaatgatcca acacgtgtat
    23761 ttgatgcgtt tggtaaaatc attgaaaaat tatttatgga ttaggaggat agtgaagtgg
    23821 attgggaacc ttatgattat tcgattgttt atgcggaacc ttatcgcgta caacagatta
    23881 caagcaattg gcgtcttcca tttccagttg cattagt
//
LOCUS       DAYYHB010000039        23677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000039 DAYYHB010000000
VERSION     DAYYHB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.040"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..815
                     /locus_tag="ACHA6B_001492"
     CDS             114..815
                     /locus_tag="ACHA6B_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8949072.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1424..2440)
                     /gene="tsaD"
                     /locus_tag="ACHA6B_001493"
     CDS             complement(1424..2440)
                     /gene="tsaD"
                     /locus_tag="ACHA6B_001493"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8949073.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2437..2901)
                     /gene="rimI"
                     /locus_tag="ACHA6B_001494"
     CDS             complement(2437..2901)
                     /gene="rimI"
                     /locus_tag="ACHA6B_001494"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8949074.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2908..3450)
                     /gene="rimI"
                     /locus_tag="ACHA6B_001495"
     CDS             complement(2908..3450)
                     /gene="rimI"
                     /locus_tag="ACHA6B_001495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8949075.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3434..4156)
                     /gene="tsaB"
                     /locus_tag="ACHA6B_001496"
     CDS             complement(3434..4156)
                     /gene="tsaB"
                     /locus_tag="ACHA6B_001496"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8949076.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4347..5327)
                     /locus_tag="ACHA6B_001497"
     CDS             complement(4347..5327)
                     /locus_tag="ACHA6B_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949077.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5351..6835)
                     /gene="galT"
                     /locus_tag="ACHA6B_001498"
     CDS             complement(5351..6835)
                     /gene="galT"
                     /locus_tag="ACHA6B_001498"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8949078.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6847..7836)
                     /gene="galE"
                     /locus_tag="ACHA6B_001499"
     CDS             complement(6847..7836)
                     /gene="galE"
                     /locus_tag="ACHA6B_001499"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8949079.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8084..8251
                     /locus_tag="ACHA6B_001500"
     CDS             8084..8251
                     /locus_tag="ACHA6B_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8949080.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8313..10124)
                     /locus_tag="ACHA6B_001501"
     CDS             complement(8313..10124)
                     /locus_tag="ACHA6B_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8949081.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGCRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10121..10486)
                     /locus_tag="ACHA6B_001502"
     CDS             complement(10121..10486)
                     /locus_tag="ACHA6B_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8949082.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10649..11044)
                     /locus_tag="ACHA6B_001503"
     CDS             complement(10649..11044)
                     /locus_tag="ACHA6B_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8949083.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11062..12024)
                     /locus_tag="ACHA6B_001504"
     CDS             complement(11062..12024)
                     /locus_tag="ACHA6B_001504"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8949084.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12024..12236)
                     /locus_tag="ACHA6B_001505"
     CDS             complement(12024..12236)
                     /locus_tag="ACHA6B_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8949085.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12257..12955)
                     /locus_tag="ACHA6B_001506"
     CDS             complement(12257..12955)
                     /locus_tag="ACHA6B_001506"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8949086.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12975..13517)
                     /locus_tag="ACHA6B_001507"
     CDS             complement(12975..13517)
                     /locus_tag="ACHA6B_001507"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8949087.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13649..14653)
                     /locus_tag="ACHA6B_001508"
     CDS             complement(13649..14653)
                     /locus_tag="ACHA6B_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8949088.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14650..15639)
                     /locus_tag="ACHA6B_001509"
     CDS             complement(14650..15639)
                     /locus_tag="ACHA6B_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8949089.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15636..16442)
                     /locus_tag="ACHA6B_001510"
     CDS             complement(15636..16442)
                     /locus_tag="ACHA6B_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949090.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16608..17564
                     /locus_tag="ACHA6B_001511"
     CDS             16608..17564
                     /locus_tag="ACHA6B_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8949091.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17641..18159)
                     /locus_tag="ACHA6B_001512"
     CDS             complement(17641..18159)
                     /locus_tag="ACHA6B_001512"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8949092.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18247..18396)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_001513"
     CDS             complement(18247..18396)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8949093.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18624..19070
                     /locus_tag="ACHA6B_001514"
     CDS             18624..19070
                     /locus_tag="ACHA6B_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8949094.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19265..21160)
                     /gene="asnB"
                     /locus_tag="ACHA6B_001515"
     CDS             complement(19265..21160)
                     /gene="asnB"
                     /locus_tag="ACHA6B_001515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8949095.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHKGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21485..22459)
                     /gene="bsh"
                     /locus_tag="ACHA6B_001516"
     CDS             complement(21485..22459)
                     /gene="bsh"
                     /locus_tag="ACHA6B_001516"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8949096.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22793..22880)
                     /locus_tag="ACHA6B_001517"
     tRNA            complement(22793..22880)
                     /locus_tag="ACHA6B_001517"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22846..22848),aa:Ser,seq:cga)
     gene            complement(23025..23591)
                     /locus_tag="ACHA6B_001518"
     CDS             complement(23025..23591)
                     /locus_tag="ACHA6B_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8949097.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         7141 a         4985 c         4041 g         7510 t
ORIGIN      
        1 attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat
       61 tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg
      121 ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct
      181 cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac
      241 agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg
      301 ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa
      361 ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag
      421 cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa
      481 aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg
      541 aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat
      601 tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc
      661 cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag
      721 taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct
      781 gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc
      841 tgataaatat cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt
      901 tttagatcac tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca
      961 ctgtttgcaa aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta
     1021 gattattcga tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga
     1081 taatgataaa taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa
     1141 aatagggctt tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc
     1201 aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt
     1261 ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag
     1321 cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg
     1381 ctgttcttat tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca
     1441 agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg
     1501 ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt
     1561 ctacctcagg gagtttagct gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg
     1621 ccgctacacc tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg
     1681 ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt
     1741 ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc
     1801 cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat
     1861 cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata
     1921 ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt
     1981 catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg
     2041 ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat
     2101 ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac
     2161 caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt
     2221 ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt
     2281 gatggcggct agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag
     2341 aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat
     2401 cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa
     2461 ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc
     2521 taatatacga aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac
     2581 ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg
     2641 ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc
     2701 atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag
     2761 ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc
     2821 atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa
     2881 atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa
     2941 cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa
     3001 tttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc
     3061 acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt
     3121 acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct
     3181 tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag
     3241 cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac
     3301 ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta
     3361 acgacgatcg atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct
     3421 tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca
     3481 accatttttc ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg
     3541 caactggttc aactaaagca cctaattgag ccacagttac accacttgga atatcccatg
     3601 ctggaactgt attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt
     3661 gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat
     3721 cagaagcaga aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt
     3781 atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca
     3841 caccgatgca attagccgcg atggttttca gactagatac tcctactagc tctttatcta
     3901 atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg
     3961 gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca
     4021 attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt
     4081 gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta
     4141 ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag
     4201 catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga
     4261 ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact
     4321 ccattttccc acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt
     4381 tccacaataa tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc
     4441 actgctagct ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca
     4501 tatttcgcta cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa
     4561 cctttttctt gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc
     4621 gcatcaggaa catgcggatt cgtttttaaa tattgcttca ctgcttgata tcctgactcg
     4681 acagaaaaat ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt
     4741 tgaaatgcca taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt
     4801 ccagcaagca tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga
     4861 acacttgact taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct
     4921 accaaaagca aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt
     4981 cctagtgcga tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat
     5041 aaagtaacgt ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa
     5101 agatcttcaa gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta
     5161 tttgctgcat ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt
     5221 tgctttgtgg ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta
     5281 gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc
     5341 ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc
     5401 gcttgaatac tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta
     5461 cgacatcggt gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc
     5521 acggacgatg atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta
     5581 ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa
     5641 tgttctcctt tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt
     5701 cttctgaaac attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc
     5761 gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata
     5821 ctgtttcatc cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca
     5881 agtttttctc tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga
     5941 tgacttgcgg ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa
     6001 attcatgacg tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta
     6061 agtcagcatt cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag
     6121 caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt
     6181 cattgtaata agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc
     6241 gaataatccg gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt
     6301 ccatgcaaag catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt
     6361 gttttggatc tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg
     6421 actctgccgg aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct
     6481 tacttaaatt aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa
     6541 agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa
     6601 tctcttgttc tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct
     6661 gatccaataa gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat
     6721 cttctcctac catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc
     6781 cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc
     6841 attcccctat tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat
     6901 aatcgctttg atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact
     6961 tgaagcgact aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc
     7021 ttttcctgtc acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct
     7081 gcctaaattg aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg
     7141 tgcagcaatc aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc
     7201 ataatcgtct ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat
     7261 tgggacaaga tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc
     7321 ggctacattg aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt
     7381 catcattttt tccatcatca atttactttc accatacgga tttttaggat tagctggtgt
     7441 atcttctgtg attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac
     7501 gattttattc acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt
     7561 gttgttgaaa tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa
     7621 atggattact ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg
     7681 gatgtctcct tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa
     7741 attatctacc acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc
     7801 gatataaccg gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa
     7861 aattgtgttt gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca
     7921 aacaaagtaa aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat
     7981 tttcaagggt tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt
     8041 tgttccataa tagatataac aaataataaa tgaggtggat tcaatgaaaa aatttgtatc
     8101 tggaatatta gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt
     8161 gaaaaaaaca gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa
     8221 aaaagcaatg cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt
     8281 tctaaaaaac agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct
     8341 attttatctg cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt
     8401 agccaatcca caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct
     8461 gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt
     8521 cctgtattat ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg
     8581 acaggattac ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga
     8641 ctgctgattt ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca
     8701 aatggcggtt cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc
     8761 cgaatgattg gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt
     8821 atttctttgt attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca
     8881 cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc
     8941 gcttctttta tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga
     9001 taggcaagag gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc
     9061 tttccgagac actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga
     9121 atcggcaagt gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc
     9181 agatagtcaa aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt
     9241 tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga
     9301 gggtaataag ggagccccct tcttgatcca ctgtatacaa tcggttcctt gcctgatgga
     9361 tgatagacca gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat
     9421 ccgacataat caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt
     9481 atcttcgaaa gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc
     9541 tttgcgtagt ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt
     9601 gctaaaacaa gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct
     9661 tttcttttgc ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct
     9721 tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc
     9781 tgatcttttt ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc
     9841 tgttcgatat aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt
     9901 accaatcctc cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa
     9961 ttcattaata actccggtgg ctgattcaac cgccaaactc gacatccagg accattggga
    10021 tcgaaaagaa aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct
    10081 acacttaatc ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt
    10141 cgataaattt tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa
    10201 aagtaatgac tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt
    10261 cttcttcgaa aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta
    10321 catggtcaaa agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta
    10381 actgcgcttt ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc
    10441 catcctcttt ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct
    10501 ttctaccttt tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg
    10561 gcacaactat ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt
    10621 ttttaaagct gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc
    10681 atccgtcata tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat
    10741 ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc
    10801 gaatagatcc taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat
    10861 gcccagcatc aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt
    10921 ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga
    10981 cttcccaaag gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa
    11041 ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac
    11101 accagcttca ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa
    11161 agtaaattct ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac
    11221 tcctccacct aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa
    11281 acctaagtag aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc
    11341 catttcaaag atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt
    11401 tgaatcccct gcgtattctt ctgccaagaa acgacctagg cgtaccacac ctgtagcgct
    11461 agatacagtt tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt caggatccac
    11521 agtgatatgt ccgatttctc ctccagctcc gctcacgccg tgaatcaaat ttccgccagc
    11581 aacgatacct ccgccaacac ctgttcctag agtaacgaat acaacgtctg gatcgttttc
    11641 tccagcacct ttccatcgtt cacctaaagc agcaacattc gcgtcattat cgatagcgaa
    11701 tttgattcct gttcctgcct cgatcagttt tttcacaaat tgaacttctg tccagtttaa
    11761 gttatacgca ccaataaccg ttccagcttc gctgtccacg cttcctgggg tacccatgcc
    11821 gattccgata aagtcttcgg ctttcatatt atataaattc aaacgatgat tgattgattc
    11881 aatgatttca ggaacgatat gcttaccatc atccaaaata ttggtatcga tgctccattt
    11941 ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc
    12001 aatcccaata atcttcttgt ccattacaaa tttcctttct ttcggctttc ttcaattcgg
    12061 tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat catgatcaat caaacggcta
    12121 tcatacagat tacgcaattc gatagtcatt aattcaatgt catagatgcg tgcacccaca
    12181 tagataaaga tgccaaattt tttgaacagc tgctgtacat catataagct ctccatcttt
    12241 aagcacttcc ttcatattat acaagtaaac catatttttt taaacccagt aaaatacata
    12301 ttacaaaaag aaatacaaaa actagtgtag aaagaatcct ttggtgtatc gaatagcttg
    12361 tctgttgttt cggtagaccc aaaacattcc ctaacactaa tccgccaatt agcccaccga
    12421 catgtcccca aacatctaca gaggtatcca gaagaccaaa aacaaatgtc aacacaataa
    12481 ataaaagaaa atgtctgact agctgctgga tcgctgggtt atgtttaaaa tgaaacccta
    12541 agataaacaa cgcaccgaac aaaccgaata tcgaagtact tgcaccacca gacagaacat
    12601 tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat cccgctgaac atataaatca
    12661 agaaaaaacg ccaatggcca tagattgcct cgatttgctg ccccataaaa tacagtatca
    12721 gtgagttcat tacgaaatgc atcagtccaa aatggatgaa gattggtgtc actaaacgcc
    12781 accattcatt gaagtgaaca agataaggac catacattcc ggcataataa gggatctcaa
    12841 gtcgcggaaa taattccatc atgaggaaga ccgctgtctg tatccctaaa aaagcatagg
    12901 tccataacgg tcgtcttaac catgcacgca attttatttg ctgttgataa ttcatgaaaa
    12961 cctcccattt cattttagtc aataaacaat tgttggatct tctgatcaaa gctctccggt
    13021 tgccattgtt cattcaattg ttcacgaaaa acaaggctac atgttttttt atgaaaatta
    13081 tttaaatacc ggtcgtaata accaccgcca aaaccgattc gataaccggc tctattgtag
    13141 ataatacctg gtacaatgag gagatcgatc atctccggca aatagagctt ctctgattgt
    13201 ggctcaagaa caccaaaact cgaacgtaca agaacggact gttcatccat ttcataaaac
    13261 gccatctgac gtttcggcaa agttttaggt acaactattt tcttttgttc tttttctgct
    13321 cgttggataa ccggctgtgt atccagttca aattcattcg ataaagtcgt ccctatcact
    13381 tttgctgact tccagtactc gctggcgaat agctgtttat agattcgctg ttctttttcc
    13441 tttttcttgc cacttttggc cagatttttc agcttttcga ttcctttccg gcgtaatctt
    13501 actttcaatt ggtccaaaaa ctcacccttc cttctctcat aaaatggtat aggaaaaaaa
    13561 gaaaaatagc aatcgaactt tcaaataaat gcataaatag aataaaaaga agacctagaa
    13621 atgattggcc ttcctttttt aatgaatttc aactgttcag cgcgtgtttt tgtctgataa
    13681 aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat caaaattcct gtaggtactt
    13741 ccccacttag tagaatcact ctagcaacca tatctgcact aagcagaaaa atggaaccga
    13801 acaaaccgct cagaacaaag gtccagttat tttctttacg tacgactagc ttcgcagcat
    13861 gaggagctag tagtcctaca aaagataaac tacctgtcac cgctacacta ccgcttgcca
    13921 aagcaacagc taacaacaaa aggatttttt gacttttagc cacgttaagg cctaatcctt
    13981 gcgcgcgttc ttgaccaaat gatagcaata caatcctctg tgcataaagt cctcctagcg
    14041 gaattaagat aaccgcccat ggaagcaatg cccaaacata aggccagctc gcaccccaga
    14101 ttgatcccgc taaccatgca ttcacaaatc cataattttc cttagataat tggatcgtgc
    14161 atagcaatgt taatgcagaa aggccggcat taacggcaat accggacaat aagattcgat
    14221 tcatacccaa aaagccattt cgatcatagg ccgcaagata gacacagcaa gcagctaaaa
    14281 caccacctag aaaggctaca actagcaata gaaacggcag cgaatagtgc gaaaagaaac
    14341 ctaaataaag catcactaaa aatcctgctc cggcattaat gcccaatata ctagaatctg
    14401 ccaattcatt tcttgttact ccttgcaaga tataaccaga aacgccaaga caagctcctg
    14461 ctaataaact gatcagtaat cgaggaactc gaaattctct aacaatcagc gccaaagaag
    14521 atgattcttt ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa
    14581 tcattaaact acctatagac aggatgacaa gtacaaaaag caagaaatag aacgtccaat
    14641 atattttttt caccgttgtt ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa
    14701 tgcaatgact aatccaaaag gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc
    14761 cgccaacata accaatccac cgccaactaa aaaagagaca ggtagaacat aacgatagtc
    14821 cgttccaacc attccgcgaa caactggcgg aaccaacagc ccaataaatg aaacaggtcc
    14881 gactaacgaa acggcaatgg ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt
    14941 tcgtttagga tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat
    15001 tgcttttcct aatgaaaaag aaaccaaaac agcaagtata tataaaggaa acaatactgc
    15061 taattgtttc catgtgatat tggcagctcc gcctacaaac caaaaagcaa gatcctgctg
    15121 cagattaaag aattgggtaa gtgcttggct gatagcagta aaaaaagaac taatagctac
    15181 gccagctaaa atcagctgta aaggattcat gcccattttc gaaaactgca aaaaaccaaa
    15241 tacgagcaaa gttacagcaa aagaacctgc caatgacatt agaaacacag aaaaatcaga
    15301 agctttaggc caaaaagcaa aactaagggc cattgccaac gaagcacccg cgttgattcc
    15361 tagtaagcct gaatctgcta aaggatttct tgtcactcct tgcatcaatg caccgctcaa
    15421 tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat
    15481 gatttggtga ctttgttttg atctatcaaa atcaaacaac gcctgccaaa aatctgcttt
    15541 tcccacagtc gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca
    15601 gaccaaaagc aggagtctat ttttcttcat agtcctcata attgatcctc ctgctctttt
    15661 ggcaacaaat cataggttag aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt
    15721 gcatcaatct caaatatttt tttcatacac tcttttgtga tcacttcttc aggtgtacct
    15781 tctgtgatca acttaccgtt tttcattgct aataaataat ctgaaaaacg tgaagcatga
    15841 ttaatgtcat gaatcgacat caagatcgtt gtttgtttca tttgattgat ttcagtcaat
    15901 aactgtagga tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc
    15961 aagatatctg tttcctgagc aagcgccata gcgatccaga cacgctgggt ttgtccacca
    16021 gacagcgttg cgatatcccg catacgaaat ccagctagac ctgtagcatc caacgcccac
    16081 tcaattgctt catgatcttg cgtgtccatg ccactgaaca gtttttgata agggtatcgt
    16141 ccgtaagata ctacttcttc tactgtcata cctgtagggt gttcactagt ttgtgctaat
    16201 aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag agatatcaga gttattgaca
    16261 atgatctttc ctgatttcac aggtagtata cgggtaaaac ttttcaataa agtagatttt
    16321 ccacttccat ttggcccaat caaactagtg attttccctt caggaatcgt aacagacatg
    16381 ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt cagctgctct taatctcgtc
    16441 atttcaccca tcctttcgct gaaaatcatt ctcaattatg acgctgtttc aacatttcgt
    16501 caagcgctac tttcatatct atagccaaat gaagcggttg acagcgttcg aaatatcagg
    16561 aataataacc aatgagaaag attctcaatt atgttaggag gaaagaaatg aaattaaaaa
    16621 caaaagtatt cacattatct agcatattgc tgctggctgg agtactgagc gcttgctccc
    16681 cctcttcaga taaagaaaca gctacaactg ccaattcgac gacttcatcc aatagtactc
    16741 atacagtcac agatacactt ggacataaag tagagattcc aaacgcgccc aaacggatca
    16801 tcggcagtta cttagaagac tatctgattg cactagggga aaaacctatc gcccagtgga
    16861 ctgttggcag tggttccatt caagattatc tgcaagatga tttaaaggat gtcccaacga
    16921 tttcttatga ccttccttat gagaaagtgt taagctttga gcctgatttg ctgttgattt
    16981 cttcttccgc tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc gcgccaactt
    17041 atgttgtaaa aaacggaaca gatgtcacat gggaagaaca actaaaagat gtcggcaaag
    17101 ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa tccatcaaga
    17161 aaactcgaga agaactagca gataaaatcg agaacaagac agctgcagtg ttgtgggtga
    17221 ccaacaactc agcctttatg gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg
    17281 atctggaatt tggtgttcct gatctagtgg aagaggtctc caaaaaagcc acttctgact
    17341 ggtctgctgt ttcctctgaa aagctagctg aattagatgc tgactacatc atccttgtga
    17401 acagtgacaa agatgctgcc atgttcaatg aaccaagctg gaagaatcta aaagcagtga
    17461 cagacaatca tgtactgcaa ttcggacctg aatctagttg gctatataac ggacctattg
    17521 ccagtcagga aatggtcaat gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag
    17581 catcttcacg attagtcgtg agatgctttt tttcttatta acaaactaga cgcatagatt
    17641 ttattcttct tttgaagcaa ccctattttt tactctttcc catttttgaa aacgatgggc
    17701 atatagattt tcttgatccg ttatcccctc atactcctct ataagggcga agtctgaaaa
    17761 atcaatattc ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat
    17821 ccgaccagtg taaggaagaa gttcttgaaa aatccgacta ccacccgaaa catagatatc
    17881 ttctgagatc gtttgactat acgacaaaag ttcatcgatc gaatgcatta cttccgcatt
    17941 ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg atatggcgat aaggaagtga
    18001 aagatccccc attccttcat aggtctttct tcccatcact aaaattcgat ccattgtgtg
    18061 ttcgcgaaag aaacgcatat catttggcaa tcgccatggt aacaaaccat ttttgccgat
    18121 cagaccattt ttatcttgtg cccacatgga aataaacata ttatccgctc cttatgattt
    18181 tagatgtttt gagataaaaa aagtctcaat tctttgacca gcaaggaatt gagaacttta
    18241 aaaacattat tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg
    18301 tttttccaaa cgatcaggat tgttacgttt gtttttgctt gttagatagt tacgttcttt
    18361 acaagaagta cactctaaag tgatgtttac gcgcatgttt ttccctccca tttttacaag
    18421 aatcctctaa gatgactctc agacttgatt atcttaccat gattttttct agattgctag
    18481 tgtttttcac gattttgtta agtaaaacta cttgacgata aatacaacgg ttttacttct
    18541 caaacaaaat ctttttcttt ttgtataaat acccttacac ttgtttatat agattatgtt
    18601 ttttaataaa gggggatact aatatgccat ggaacatgaa ggactttcct gcatcaatga
    18661 aaaacttaga taaattgaca agaaaaaaag caatcgacat cgcaaatgca ttattagatg
    18721 aagggtaccc tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa aaatggtatg
    18781 aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca agcaagacgg
    18841 atgaacacga tacgaatcct cgtgctggaa aactgcttga ttcagatgtg atcgtagaat
    18901 atgaggaaga acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc aaccattttg
    18961 atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa gaatcaactt
    19021 tgttcattta taaaaaagat gggacaaaag aaaaagaaat cgattattga acatatgtaa
    19081 aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg gtgttcctct
    19141 gccaactccg aaccataagt cataatatct ataaaacggg aagagctgtt ttttgaaatt
    19201 gtttcttatc actcagtgtt aaacggtagg agaaaacctc ttttttattg atatttgatt
    19261 ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca tctacaaagt
    19321 agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc tcttctttac
    19381 gtcctttgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa aattcttttg
    19441 caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct ttcaaccaag
    19501 ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc ggaagatgtt
    19561 tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataaa aacttacttg
    19621 gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg acttccaaag
    19681 aacttgccat agaaagttta tctgccttta acaagatatc ttttggcatc cattgatgaa
    19741 gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc tcgtaatgaa
    19801 cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgttaac acttctgatg
    19861 cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca ggagcaagtg
    19921 attcatataa atggatccgt ccatggaaat ttttcatttt tccgatttta cgtcctaaat
    19981 tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg atgaattttg
    20041 aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc ccttctccag
    20101 attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa ggtacgcaag
    20161 aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct ttgaacgaca
    20221 tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt agttttctgg
    20281 cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa tgatctggtt
    20341 tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa ccaacttcta
    20401 catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt ttttcgatcc
    20461 attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat tgctgaatgt
    20521 ctatttttcc atctttatac gtataataat ggccttcagg caaacgatag acatctttga
    20581 agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc aacgcttctt
    20641 tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct gaaccaaaca
    20701 taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg tctcttgcac
    20761 cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg cggatctttt
    20821 gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta tctgcatgtg
    20881 tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa ttatagattt
    20941 cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga gtaccgccct
    21001 ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg atatattctc
    21061 ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata attgtttttt
    21121 tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca catcctaata
    21181 taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag cggacgaata
    21241 ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg tttttacaga
    21301 aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc ttattctctt
    21361 cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa aagacacgac
    21421 ctaaagatcg tgtctaatta tgactgtatc agaaaatatg ataccaaacg gatctattca
    21481 ttaattaatt gacatagttt atctgttgct cttttatgac tggataactg accaattcat
    21541 gacgatccaa gtcttcttta ttcatattta cagcagtaat ttgactgtct tcatacgtac
    21601 gataataata aatcccttta tcaacgttac agcaagaaga ataaatcgta tattcgtatt
    21661 ttctttctcc agtatcgcat actccttttt gttgctcgac tgagcctagt atatgaaaaa
    21721 attgactgat actttcagat tctgaatcac ctgatgcagc attcatttta gtaaatgtgg
    21781 cttttacaaa tcgagaaaca gaagagagat ctccaggtaa gcccaatcct cccatcccac
    21841 gactataaac attcaagctg atctggtttg aaaaatgatt ttctggtgtt ccatttgata
    21901 gcgagcgata attgttcaga ttaaataatt gataatcaaa tggcggatta tttgtcaaca
    21961 caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt actatcgatt
    22021 tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca ctaaaatcga
    22081 tattgactag attgatagta gccaataatt ctttagcttc gtctatggtt gagcattgcc
    22141 ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct tgtaattctt
    22201 tataatcagc attgccagga aaattcagtc ctgccatact caaccctttt tcatttgtag
    22261 cctcataata gagtggatag ttggataccc cagctgctat tcctatcatt gcataatgtt
    22321 tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta atagtgacca
    22381 cttcattata agacatctca taatcaaaat tccttccaaa ataatggtct ttcgttgcat
    22441 aagtgattgc ggtacacatc gtttcccgtc ctaaaacaaa tcgtttttac acttttatct
    22501 tacatctagt atttttttta tcaaaaaaag gccaaagaag attgatttac tagcatttat
    22561 aaaaaatagt actcaggggg aatcaaaaaa tattgtaggt taaaagtaca acaataaggg
    22621 gctgtgacaa aagtcttgtc acagcccctt attccgaaca aacggtgttc aaaagcttgc
    22681 atctgcggta ataagcccaa tcaaacgaaa aaataggaaa agctattttt gtttggccgt
    22741 tcttattact tgatgcagga cagcttcttc acaacctcta atcgttgata taaggaaggt
    22801 acaggatttg aacctgcgcg ccggtattag ccggttcgcc ggatttcgag tccggtgcat
    22861 taccactctg ccaaccttcc gtaagtacta tttttaaact tttttcacga aatgtcaagc
    22921 ctatttctaa tagataaata acagaaatcg ctttttttag caaaaaagtc cattgacagt
    22981 ttgcagccaa cggacttacc gatcaaattc atactgatag aacactatct gattatttca
    23041 ggattatcta atgctaaaat cgagtcaatt gtccgtttga tcagtgcaat gatttgttct
    23101 tctgttggat gcatgatgtt tgatgacatc aatgacgcta agccagtcac tgctacccat
    23161 gtaccagtat gcagagcatc gacatattca tccgataatt gattgtatct ttcatcttct
    23221 ttaactattt cacggaaata attgtatgaa aaatcatgca ttttctttcc gtccccatgt
    23281 tcatcaatgt acaatgccat gtaaagacta tgatgctttt ttgcaaagta gatatagttc
    23341 aaaccgagat caatgatttt atcaccagta cgttcaatag ggaaaacatc ttcttttagt
    23401 tgttcaaaga tagaatccag caacgtattt tttaaatctt ccatgttttt gaattccaga
    23461 taaatcggct gtgtcgaaat ccccatcttt ttagcaatat ttctcgcagt aaatccttta
    23521 aacccttcct ttgcaacaac atcataagca gcttttagaa tttgatcctt ggtatagact
    23581 tttcttctca ttaaaactcc cccacttctt tactgcagta tatgttgttt tattgtaccc
    23641 tatttttagc attttttgca aacattttac tcaaaac
//
LOCUS       DAYYHB010000040        23612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000040 DAYYHB010000000
VERSION     DAYYHB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.041"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..767)
                     /locus_tag="ACHA6B_001519"
     CDS             complement(<1..767)
                     /locus_tag="ACHA6B_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8949098.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
     gene            878..1432
                     /locus_tag="ACHA6B_001520"
     CDS             878..1432
                     /locus_tag="ACHA6B_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949099.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1605..2078)
                     /locus_tag="ACHA6B_001521"
     CDS             complement(1605..2078)
                     /locus_tag="ACHA6B_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949100.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2090..3037
                     /locus_tag="ACHA6B_001522"
                     /pseudo
     CDS             2090..3037
                     /locus_tag="ACHA6B_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3308..3743
                     /locus_tag="ACHA6B_001523"
                     /pseudo
     CDS             3308..3743
                     /locus_tag="ACHA6B_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3759..4592
                     /locus_tag="ACHA6B_001524"
     CDS             3759..4592
                     /locus_tag="ACHA6B_001524"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949101.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4837..5862
                     /locus_tag="ACHA6B_001525"
     CDS             4837..5862
                     /locus_tag="ACHA6B_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949102.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6000..6125
                     /locus_tag="ACHA6B_001526"
     CDS             6000..6125
                     /locus_tag="ACHA6B_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949103.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6150..7265
                     /locus_tag="ACHA6B_001527"
                     /pseudo
     CDS             6150..7265
                     /locus_tag="ACHA6B_001527"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7348..8244
                     /locus_tag="ACHA6B_001528"
                     /pseudo
     CDS             7348..8244
                     /locus_tag="ACHA6B_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8260..9084
                     /locus_tag="ACHA6B_001529"
     CDS             8260..9084
                     /locus_tag="ACHA6B_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949104.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9116..10426
                     /locus_tag="ACHA6B_001530"
     CDS             9116..10426
                     /locus_tag="ACHA6B_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8949105.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10574..11833
                     /locus_tag="ACHA6B_001531"
     CDS             10574..11833
                     /locus_tag="ACHA6B_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8949106.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11843..12715
                     /locus_tag="ACHA6B_001532"
     CDS             11843..12715
                     /locus_tag="ACHA6B_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949107.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12727..13557
                     /locus_tag="ACHA6B_001533"
     CDS             12727..13557
                     /locus_tag="ACHA6B_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949108.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13597..15780
                     /locus_tag="ACHA6B_001534"
     CDS             13597..15780
                     /locus_tag="ACHA6B_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8949109.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15841..17061
                     /locus_tag="ACHA6B_001535"
     CDS             15841..17061
                     /locus_tag="ACHA6B_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8949110.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            17058..18518
                     /gene="gtfA"
                     /locus_tag="ACHA6B_001536"
     CDS             17058..18518
                     /gene="gtfA"
                     /locus_tag="ACHA6B_001536"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8949111.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18595..19701
                     /locus_tag="ACHA6B_001537"
                     /pseudo
     CDS             18595..19701
                     /locus_tag="ACHA6B_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            20023..20232
                     /locus_tag="ACHA6B_001538"
     CDS             20023..20232
                     /locus_tag="ACHA6B_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8949112.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20225..20374
                     /locus_tag="ACHA6B_001539"
     CDS             20225..20374
                     /locus_tag="ACHA6B_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8949113.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20528..21193
                     /locus_tag="ACHA6B_001540"
     CDS             20528..21193
                     /locus_tag="ACHA6B_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949114.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21477..22562)
                     /gene="xerS"
                     /locus_tag="ACHA6B_001541"
     CDS             complement(21477..22562)
                     /gene="xerS"
                     /locus_tag="ACHA6B_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8949115.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22896..23129
                     /locus_tag="ACHA6B_001542"
     CDS             22896..23129
                     /locus_tag="ACHA6B_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8949116.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23126..23533
                     /locus_tag="ACHA6B_001543"
     CDS             23126..23533
                     /locus_tag="ACHA6B_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8949117.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         8005 a         3560 c         4528 g         7519 t
ORIGIN      
        1 ttacgagata catggataca acaatgctga tattttgctt taggataaat ttgatggata
       61 gtatctttca tgccttttaa gccgtccgta ataaaaagca agacttcttg aactcctctg
      121 gagttaatat cctgtagcag ctcattccaa acgtatgttg attcagttgg agcaatcgca
      181 taactcagta cttctttagt gccgtcttct cgtataccaa tggcaatata aatcgcttct
      241 ttggatacgg tttgacgttt tagtggaatg taagtagcgt ccataaaaat agcgacatac
      301 ttatcattta aggctctgga tttaaaggca tttacttctt cagtcagaac tttagtcatg
      361 ttggacatgg tttgtggagt atagtgatga ccgtacattt tttcgatcaa atcagcaatt
      421 tcagacatcg taacaccttt ttcgaataaa tggataatag tggtttccaa tgtatcgttt
      481 gttcttttgt aggctggtaa agtttgttgt ttaaactcac cattacgatc tctaggtatt
      541 tccaatgtta attcaccata ttcggttttg attgatcgaa agtaagaacc gtttctcgaa
      601 ttacctgaat taaaaccagt gcgatcatat ttttcgtaat ctaaaaaagc cgttaattca
      661 gtccgtagga gtgtgtttat cgctttttct aagtgcgaac ggaataattc atttaaatcg
      721 cctttagtga ctagagtttg cacaatttct gtagtaaaat cattcatagg gaagtcctct
      781 tttctgtgaa ttggttgtcg ttaactttat tctacagaag ggacttcctt ttttgtatgg
      841 attttttcat ttacacaaaa tattttatac tctcatagtg agctataatg tacaaccgtt
      901 aaggacaaat gaagagatac aagattttca gttttggcta agaagaacaa gtaatccgga
      961 aagagatagt tttttatttt tgtttggaat caataatggg ttacgtatgt cagatattat
     1021 aggattaaaa gttggagata tacgaggcaa aagtaagcct attattgttg aacataaaac
     1081 aggtaagcga aaaccgattt ttatagataa tttgagagaa gaaattttat tatatactga
     1141 aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa caaggtcgac atataacaag
     1201 ggatcgtgtc tatcagatat atgcagatat agcagaaaaa ttaggacgag atgatatagg
     1261 aacacataca ttaagaaaaa cttttgggta tcactactac aagaaaacta gagacattgc
     1321 taccttaatg tttattttta atcatagctc gcaagcaatt acaaaaaggt acattggtat
     1381 aactgaagat gaaataggag catcacttcg tggatttaaa ctaggtgtat aatttaaaaa
     1441 tttttataaa cagtaaagac aaagattaaa agctattttt atctagaacg attttatttc
     1501 ataaagttcg cggaataaaa gctggggtta ctggtacttg gtataggttc gtgttatata
     1561 aactaagtaa ttaaataaca tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc
     1621 aatacggtct caaaaacgca aacgctgcgg aataagaaaa atactgataa ctcaacgttt
     1681 agcagactat ctaataggga tagtctgctt ttttcgtttt tgaccatact tttgaccttc
     1741 tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt
     1801 tcctctgtct gctttttcgc gatatgggtg tatatatcat ccatagtggt tttaatatct
     1861 gtatgtccaa gtcgaacata cacttctttg acagacgcac cagattcaaa gagcagacta
     1921 caatgggtat gacgaaaacc atgaagtgtg atatgtttta agttattctc tttaatgatg
     1981 attttcaaat tgtgatttac atagtctaaa taaaggggct tattttcttc tgatgaaaac
     2041 aaaagttgtt caccatcttt tataggtttg attctcattt ttagatatta tgcccgcacg
     2101 tattcatcac atttctattg tgaatcgctt tattcgaccg acatttgact tttatcataa
     2161 tatactcggt ttaaaattac taatgaaaac aattaatcaa gacgatcata ccatgtatca
     2221 cctgtttttt tcagataatc accaccgtgt gggaactgaa ctgacttttt ttgaagtaca
     2281 agaagggaac aaccagtttt ttggaaccaa tacgattgaa cgaacgatac taaaagttcc
     2341 ttccgaagca tcattgcatt tttgggaaat atattttgaa acacaaggcg tttgccatta
     2401 tggaatcgaa tcattttctg gacgacctat cttacgtttt gaagggccag atgcaacacg
     2461 attagcttta gttcccttac gcgaatttga agatattgat gattatttcc cgtatgtcac
     2521 agaccagatc cctacagagc atgccatttt aggaattgat gccattcaac tacgcgtaca
     2581 atattccgat gcgactgaac gagagttatt gtcggtaggc tggtagcaca aagaatacgt
     2641 cagtttcttt tcgggtcatc aacgagtgac aatcttagaa aatcaagaca ctcagttcta
     2701 tcaagaagtt cagattattc aagatcgtga gaatccagtt gccattgaag ggattggtag
     2761 cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca
     2821 attacaagag cgcaatttta taaattcagg aataaaagat cgtgaatttt ttgtatcact
     2881 ttattatcgt gacccaaatc aattattgat tgaaattgct acaggggaag gaaacctaga
     2941 tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa attcctttat ttttaccgca
     3001 gtacttaaat ttcattcgtg aacaagtgga gcaataatta gctaatcaaa tccatgacga
     3061 agagtaagag gttgactcat aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa
     3121 agaatgttga tttgatgcgg ttcctatttt tataaactat cattttcgac taataaaact
     3181 acttatgagt cagcctctct ttttttcttg atttttatac ctctttctag gtaaactaaa
     3241 catctgccaa aaataccgtg tctcattgaa acattctcaa ctatttcaaa ataaagggga
     3301 gtaccaaatg aacgagtata ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca
     3361 gtctcaaaat tcccaaaaat aaaattagta tttttaccag cgtatctggt tcaggaaaat
     3421 catcgattgt ttttgatact attacccaag aagctggtcg tcaactgaat gacacctatg
     3481 atagttttac ccgtttattt ttacctaaat acagtcgtcc agaggttgat gagattgata
     3541 atctttctac ggcgattgtg gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt
     3601 tagggacgat tactaacatc aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac
     3661 ctagtttagg atatgcaaat gcttattcat ttaatgatcc tagtggaatg tgttctgtct
     3721 gtcagggaat cggtaagacg attgcatttt taataggatt gctttatttt tcggctattg
     3781 ataatgctga ttttgtattt tctgatccat ttaccgcaga agttttgtct ggaattgagg
     3841 aacaggcaaa gaatcatggt tattttgtat tggtgcataa tgttaattca gcagaagatg
     3901 ttaaaactat tcagagaaat tggcgttttg aggggttcat tgttgttggt gtacgtgtaa
     3961 aagaattttt ggaattaaat aacgtttttg atggtcctgt tgtttatatt gatacgcatc
     4021 tttcaaaaga gacattaaga gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa
     4081 atgattacca agctagtaca ttggcagtgg aacatttgat tcattatggt catgagaaaa
     4141 tagcgtttct ttcttttgac tacattgtag gtgaaccaag tgttattcaa gaacgacatc
     4201 aagcttatat cgatacatta aagaaatatc atttaacctt taaatcgcat tggatatata
     4261 acaactctga atttgagcga atttatcaag aattagctga atttactgca attgttgtta
     4321 ctggagatta tttagcggct aaattagtaa aatttttaaa aaataagaat aaacgagtga
     4381 tggaacaatt atcgattatt ggatttgatg atattacttt tgcagaatta atggatcctg
     4441 cgttgacaac aatacgacta aatccaggta acaagggggc gattgctttt caccaactga
     4501 ttggactggc caaacaagaa gaaataaaaa atcaagtact actattagag ggagaattga
     4561 tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa
     4621 caataaaata tccgaacgat agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa
     4681 ttcactagaa ttagcattat ttactctaaa ttgttcggat attttttctt gtttttaatt
     4741 atgattacag tgcctatttt taacgaaatt tgcttatttt caaaatgtat gattatatgt
     4801 tagtaaacac tttcaaatat gaaaagagga gctattatgg taggaattag ggatgttgca
     4861 aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc taaatgagga tccgacactt
     4921 tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg ttaattttta tcattatgtc
     4981 aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat taataacgac agtttctgaa
     5041 gtagatgagt tggaagatcc ctattttcga acgattcgca gagggattca agtcgaagca
     5101 gaacaaagca aagtcaattt gaagaaaatt attcgtttgt cagaaacacc gttggagcaa
     5161 gacgatttgc taaaatgtca aggtgtgttg attattgggc aggtgctatc ttctgtgatt
     5221 gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg atgaccctag tattgaagct
     5281 gaagtagatg cagtttatac tgatttaaca aaagcaacga atcagcattt agatcgattg
     5341 tataaaaaag gacaccgaaa cattgcgttt atcggaggag gacgtgttcg tttagatagc
     5401 caaggaaata aacacgtgtc agatgatgat tatcgaaaaa tagcctatga gacgtggatg
     5461 aaacaaaaag gattagaaaa atatagtcaa atttatttag aaggctggac cacacttgac
     5521 ggaatgcagg cagcagattt acttttgaaa aaacatgcta aagataagtt gccgactgct
     5581 atcatggtag gaaatgatcc tattgctgtg ggggtttatc gttcacttca aaaaaatggt
     5641 ctacatattc cagaagatat ttcgattgtg agttttgata atattgaagt tgctgaattt
     5701 ttaacacctg cattaaatac ggtgaatgtg aatacagaag aaattggaaa actagcagtt
     5761 cgcatggtta atgaacgaat aaaagaagtt agaaatgttt cgatcaaagt gattgtttct
     5821 aataatatta ttgttagaga aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa
     5881 aagggtgttt acttttttgt ttactaatat ttttagtaaa aaatctcgac aaaacattaa
     5941 gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt caaaacagga ggaattaaga
     6001 tgaaacattt caaaaaaata gctttaacag ggatagctgt tttaactggg acattactcg
     6061 cagcatgtgg tgggggagat agtaattcta aagacgggca agtaacttta agctatgcga
     6121 gttgagatac tgaccaagct ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa
     6181 atcctgatat caaagtagaa atggagacaa cgccttggga tcaatattgg gttaaattag
     6241 aagctgcaac aacgggaggt aatatgccag atgtagtaac catgcactct agcgaatcct
     6301 ataaatatat gtcacagaat gctttgatgg gacttgatga tgttatcaaa gaaaatgata
     6361 ttgacatggg aaactttact gaaggaattg ctgatttcta tacgcttgaa gacgaactat
     6421 acgcaatccc taaagatgct tctgtagtgg ggctatggta caacaaagaa ttatttgata
     6481 atgcaggtgt cgattatcca gatgaaacat ggacatggga aacactcaga caagctgcaa
     6541 ttgatttaac agatgaatca aaaggcatct atggttttgc atcacatatt ggaacagaga
     6601 ctggctactg gccatttatt tatcaaaatg ggggagaagt atttgccgat gacaatacaa
     6661 aatcaggttt gaatacccca gaagcacaag aagctttagc tttttatcga gatattgcac
     6721 ttgttgatca tgcttcacca acaattacag aattacaaga tcctgataag ttaagtcgtt
     6781 ttcaatcagg tagattagca atgatgattg aaggaaactg gtttgcaccg ggatttttga
     6841 aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt acctaaggga aaagttaatg
     6901 caacaattac aaatggactt ggttgggctg ccagtgcgag tacaaaatac ccagaagaag
     6961 tgaaaaaact aatggcattt ttagctagtg aggacgccaa taaaatccaa gcggaaacgg
     7021 gggcatcaat cccagcacta aaaggctttg aagacgagtg ggctgcttct tcagatacaa
     7081 ttgatttgag tgcttttgga aaaatgttgg agtatggagt ccctcgtcca tacaataaag
     7141 atggattacg agctgaacaa gcagaagttc aaattattaa ccgtattctg tctggtgaag
     7201 taactgttga agatggaacc gctcaaattg aaaaagaagt caatgcgata ttgagcggaa
     7261 attaaattag ttaattatac aatatacagg cgaaaggatt ctatttcgtc tgtatattaa
     7321 tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa
     7381 gatcaagtga tatggggcta tatttttatt tttccaacgg cattaggatt aattttatta
     7441 aatatatggc cagcaattca aacagcaatt ttaagttttc aaaaaacggt agggttcggt
     7501 gatactgaat gggtcggatt aagaaattac caaaaaatgt ttgcagacgg tgaagttttt
     7561 cagtctttat taaacacatt aatttatgca gtggtttcga tacctagtat tgttgtatta
     7621 tccttgttgg tggcagtttt aatgaataga aaagttaaag gattgtcgat ttatcggaca
     7681 atttattttc taccagtcgt tgctgcacca tccgcagtcg caatgatttg gcgctggatg
     7741 ttcaataatg attatggact gattaataac atgttggcta aaataggtct cgatggacca
     7801 gcatggttaa cggatccatc gattgcgatt tattctatta tcattgtagg agtttggagt
     7861 gctattggtt ataacatggt gttattacta gccggcttat aagaaattcc taaagattat
     7921 tatgaagcag cggaaattga tggtgctagt ccaatccagc agtttttcaa cattacgtta
     7981 ccattagtta caccaacatt attttttgta gttgtaacaa cagttatcaa tgctttccaa
     8041 gtatttgatg ttatttttat gatgattact cctaatagta ccgccatgtt taaatcacaa
     8101 tcattagcct atttattcta taaacattca tttattttaa atgacaaggg atatggttcg
     8161 gcaatcgtta tgttcctttt agtgattata ttaattatta ctgcaattca aatgaagttg
     8221 cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta tgaaaaataa atttttttca
     8281 aatgcgccaa tccatatcat gcttatttta ggtggtattg caatggcatt accttttatt
     8341 tggatgattt taacttcagg gaaaacgttg gttgaatcta cccaaattcc acctacaatt
     8401 ttccctgaga aatttcagct aagtaactat caacaagtac ttgatttatt accattcatg
     8461 gctttttata tcaatacatt tttaatggtt gttggacgag tgattggctc ggttttattt
     8521 tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc
     8581 agcttagtac ttattcaaat gatgattccc ggttccattt ttattactcc acagtattta
     8641 ttagcacaaa aattgggtat tttaaataca attccagcat taatattacc aggggtagtt
     8701 agtgcgtttg gtacgttttt attaagacaa tttttcttag gtttacctga tgaattggag
     8761 gaagcggcct atttagaagg tgccaatatt tggacgatct ttttcaaaat tatgttgccg
     8821 cttgctaaat caggattggt tgcacttgga atattcacag cgttgtttgc atttaaagat
     8881 ttaatgtggc cattgattgt caatacttca attgataaga tgcctttagc tccgggatta
     8941 gcaaacttac aaggacaata tgccacgaac ttcccacaat taatggcagg ttcgttatta
     9001 gcaatttggc caatagttgt gatattcatc gttttccaaa gaaaattcat tgaaggaatt
     9061 gctacaacag gtagtaaatt ataaaactat taattagtga ttggagagta agtacatgac
     9121 aaaaattaca tttattggtg caggaagtac tatttttgca aaaaacgtat taggcgatgc
     9181 gatgttaact ccaagtctac aagatgcaca tattgcattg tacgatattg atgaaaaacg
     9241 actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc
     9301 tgatattgtt tcttattatg atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa
     9361 tgctattcaa gtgggtggtt ataaaccttc aacagtgatt gattttgata ttcctaaaaa
     9421 atatgggttg cgacaaacta ttggtgacac catggggatt ggtgggattt ttagaaccct
     9481 acggacacta ccagtaatgt tagactttgc taaagaaatg gaagaagttt gtccaaatgc
     9541 ttggttattg aactatacaa atccgatggc gatgttaacc atgggtgttt taaaagcgac
     9601 gagtattaag actgttggtt tatgtcatag cgttcaagta tgcgtaccag aattgtttga
     9661 gcatttagga atagccgatc aatatgactt aaatgacttt caatggaaaa ttgcaggcat
     9721 taatcatatg gcattcttgt tggaaattac aaaaaatggg gaagatttct atccaacaat
     9781 tcgtcagtta gctgcagaaa aaccaaatcc acatccagat tctgtacgtt ttgaattgtt
     9841 gaaacgtttt ggttactata ttacagaatc aagtgaacac aatgcagaat accatccata
     9901 ctttattaaa tcaaaatacc cagaattgat tgacgaatta gggattccgt tggatgaata
     9961 cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc atgcgagagg acattgtaaa
    10021 taatggggag ttaacacata cgagaagccg tgaatatgct tcatatatca tggattctat
    10081 tacgacagga actccgtcag tgattgcagg aaatgtctta aataaaggct taatcacaaa
    10141 cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc
    10201 aacctatgtc ggtgaattgc caacacaatt agctgcttta aatcggacga atatcaatgt
    10261 tcaagaactg acagtagaag ccgctttaac actagataaa tcaaaaattt atcaagcagc
    10321 gtatttagat ccacatctat catctgaatt gtctttaaca gatatcaaaa atttagtgga
    10381 tgatttaatc gaagcacatg gggactattt acctgaatat aattaaaaag aagctgaaag
    10441 agtaaggaat tttcaattcc ttactctttt cttagttaaa tttagtaaaa atattgacaa
    10501 aacacttagt aaacagttac attagtgtta acaaaaagaa aacgcattca aattgtataa
    10561 ggaggagcat aaaatgaagt ggtctaaaaa aattatttta gcttgtatag cactaggagc
    10621 aacgatctct ttgactgctt gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat
    10681 agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt caggagattg ctaaagattt
    10741 tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt
    10801 aattaaaaca cgtatgcttg caggcgatgt accagatgtc attaatcttt atcctcaaag
    10861 tatagaattg aaagaatggg cgaaagctgg ttatttagag gacttaaccg aagaaccgta
    10921 tttagagaat atcaaaaatg gatatgcaca acgttttgct attgaggatc gtgtatatag
    10981 cataccatta accgctaatg tctatggttt ttactataac aaaactgctt ttgaggaaat
    11041 ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa atagtagctg agattaaaga
    11101 tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc tggactttaa atggttatca
    11161 ccaactagct atagcgactg ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt
    11221 ctcagaagtt aatggaatta atgctgaaag caaagaaatg caaaaagatt ttaatcgatt
    11281 agatttgtta agagaaccaa aagctttgca aaacaactgg caaggtgccg gttataacga
    11341 tacagtagtt acttttacaa aaggtgatgc gttgattatg ccgaatggtt catgggcaat
    11401 gccgatgatt cattcacaaa atccagattt tgaagtagga acttttcctt ttccagcaga
    11461 tgaagcaggt caaagtctaa cgattggcgc aggagaccta gccttatcag tttctgcaac
    11521 ttctcaacat aaagaagaag ccaaacgttt tgtagaatat atgacaacac ctgaagcgat
    11581 gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac
    11641 tcaggattca ccattaagtg gtttaactga acttgctttt actgatcgtc atttagtttg
    11701 gttagccaaa gattggaata gtgaaaatga tttttatacc ttaacaacaa attatttaca
    11761 caatggcaat caaaaagcca tgattaacgc attaaatgct ttctttaatc caatgaaagc
    11821 tgacggaagg taggaaaata aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat
    11881 catttttgta gcaattccaa ttattttgca gatgattttt ttctactttc caatggttca
    11941 aggtgctttt tacagtttaa ctaattggac tggtctgact tataattttg attttgtggg
    12001 tttgaacaat tataagattc ttttttctga tccgaagttt atcaaatcga ttggcttcac
    12061 tttaattgtg acgctttgca tgattgctgg acaaattggt tttggtattt tgattgcgcg
    12121 ggcgttgaac gcaaatatta aagggaaaac attctttaga gcatggttct tttttccagc
    12181 ggtcttatcg ggcgtggttg tttctttagt atttaaacaa atttttaact atggtttacc
    12241 aaaaattggt agtatgttag gcattgaatt tttacaaacc agtcttttag gaacaacatg
    12301 gggagcgatt tttgcagtaa tttttgtttt actttggcaa ggaattgcca tgccagtcat
    12361 cattttctta gcgggttttc aaagtattcc acaagaaatt attgaagctg ctagaatgga
    12421 tggggcgaat gataagcaaa ttttttggaa tattgaaatc acctacttat taccaagtat
    12481 ttcaatggta tttattatgg cattaaaagg tggcttaaca gcatttgatc aaatttttgc
    12541 tttaactggt ggtggaccaa gcgatgccac aacatctttg gggttacttg tttataatta
    12601 tgcttttaaa aacaatcagt ttggttatgc gaatgctatt gctttagtct tatttatctt
    12661 aattgggatt gtttcactgg cacaattaaa attatcgaaa aaatttgaaa tctaggaggt
    12721 ttcccaatga aaacatcaaa agaaaaaaat atttctaaat acttgctttt attattaggg
    12781 ggcttcatga ttttagttcc cttagtttca acgctattcg gttcatttaa aacaacaccc
    12841 gatattatga ataatttctt tcgttttcct aatccagcga ctttggaaaa tttccgaaga
    12901 ttgttagcgg atggaattgg tcattacttt tggaattcgg caatcatcac aatcattgca
    12961 gtagttgctg tgacagcaat tattcctatg gctgcttatt cactagcaag aaaaatgact
    13021 cgtgggaaag catacggatt gatgtattta tttttaattt taggaatttt tgttcctttc
    13081 caagtaatta tgattccgat taccgttatg atgagtaaaa ttggtttagc aaatatgtgg
    13141 ggattaatct tactctatct cacttatgcc gtaccacaga cactattttt atatgttggt
    13201 tttattaaac taaatgtgcc tatcagtctg gatgaggctg ctgaaattga tggggctagt
    13261 cactttacaa cttatttcaa aattgttttc ccaatgttaa aaccaatgca tgcgacgact
    13321 attattctta atgctctatg gttttggaat gatttcatgc taccattatt aattttaaat
    13381 cgtgattcta atatgtggac actaccattg tttcaatata attatacggg acaatatttt
    13441 aacgactatg gtccaagttt tgcttcctat acagtaggaa ttgtcacgat tacactagtt
    13501 tatcttgctt tccaaagaag tattatagct ggaatgagca atggagcagt gaaataacca
    13561 ataaattatt ctcattataa aggagttcat aaacttatga ttatatttga caatcacaca
    13621 caaacatttc atttacaaaa taaaaaaatt agctatctat taggaatcga agaaaataat
    13681 tatttaacgc atctttattt tggaaaaaag attacgcact attctggtgg gtatcgctac
    13741 ccccgaacag atcgttcctt ctcacccaac aatgcaaatg cgcaagatcg tttattatct
    13801 ttagacacat taccattaga atttccagga tatggacatg gtgattttag agaaccggcg
    13861 cataatatta aattaggaaa cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa
    13921 attcaaaaag gaaagcccag cttagcggga ttgccagcct tatatacgga agatgatgat
    13981 gaagctgaaa cgctgattat tcaattgaaa gacgcgatta gtggattgac tttacagttg
    14041 ttctacacaa tttatcaaaa tcacagtgta attattcgtt ctacgcaatt agtaaataat
    14101 agtgatgaaa cagtggagat taaccgctta gctagtcaat caattgattt tccaaatcgt
    14161 ccactcgagt taattcattt aaatggtacc tgggcaagag aacgtcaaat gactagagaa
    14221 aaaattcata cagggataaa agtattagat agtaaacgcg gatcaagcag ccatcaacaa
    14281 aatccgtttg ttgctttagt agatgccaaa acagatgaat ttcaaggaga ggcatatgga
    14341 tttaatttag tttatagcgg taatcacgaa acggtgattc aaaaagatcc atttgaccaa
    14401 acacgggtca ttatgggaat taattcattt aattttggtt ggcaactaaa aactggcgac
    14461 tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg aaggtctgaa tacgttatca
    14521 cagacgtatc atgaattgta taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa
    14581 cgtcccacgt taatcaacaa ctgggaagca acgtattttg attttaacga agaaaaaatc
    14641 atgaatattg tcgatgatgc gaagaacttg ggcattgaaa tgtttgtttt agatgatggt
    14701 tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg attgggttga aacagaaggc
    14761 aaacttgctt gtggtttgga aaatttagcc aagcaagtgc atgataacgg gatgaaattt
    14821 gggatttggt ttgaaccgga aatgatttct gaagatagtg aactatttcg agcacatcca
    14881 gattgggcat tgggtattcc aggtcgtgac cgctcaatta gtcgaaatca atatgttctt
    14941 gatttttcaa gacaagatgt tcgtgataat atctatcaac aaatgacggc gatttttgat
    15001 cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa atatgacaga agtctattct
    15061 gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc gttatatgtt agggttatat
    15121 gaatttttag aaaagttaac aacgaattat ccaactattt tatttgaaag ttgttcgggt
    15181 ggaggcggtc gttttgatgc aggtttcttg tattacatgc cacaaacttg gacgagcgac
    15241 aataccgatg ccgtggctcg tctgaaaatt caatatggaa ccagcttagt gtatccaatt
    15301 tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt
    15361 ttaactattc gtggcaatgc tgcgatggct ggtgtttttg gttatgagct agatctaacg
    15421 caattaactt ctgaagaaaa agcagaaatt aaagaacaag ttgcctttta taaaaagcat
    15481 cgccaacttt tgcaatatgg aactttttat cgcttggaaa gtccgtttga aggcaatgat
    15541 actgcatgga tgtttgtctc aaaagaccaa acagaagcca tggtctttta tttccgagta
    15601 ttagcagaag ctgcgcatcc attagtcaca ttgaaattag caggattagc agaagaagca
    15661 acttatcaaa tggaagatgt aacatttgct ggcgatgaat tgatgaatct cggtttttat
    15721 attgactcag aactacatgg tgattatgcg acacaacgct tttatttgaa acagatatag
    15781 tagcttgaat ttacaatata agtgcaacac caaaagagac tcattccaaa cgaagaaaat
    15841 atgttttatt tacacgcatt tactaaaaag cagcctgatt tgaattggga aaatgaagaa
    15901 gtacgtgaag aaatctatgc aatgattaac tattggttag ataaaggact aggcggattt
    15961 agaatcgatg cgatattaaa catcaaaaaa aaactagagt atggcatctt tgaatctgat
    16021 ggagaagatg ggttagcctt cattgggccc tggattctta atcagtcggg cattgaagtg
    16081 tggttaaaag aaatgagaga gcggacattt aaaccacata atagtatgac ggtagcagaa
    16141 gccgatgttc ctaatgaacg tttagatgaa tatattggtg aagatggcta ttattcgatg
    16201 gtttttgatt ttagttatac ggatattgat gtacctgaaa ctggtgaatg gtttaaagat
    16261 agccaatgga catggacgga tatgcgtacc aatattttta cgaatcaatt agttacgcaa
    16321 gataaaggtt ggggcgcctt atatttagaa aaccacgatc aaccacgttc aattaataaa
    16381 tatattccag aagaatggat caatgattat agtaaaaaaa tgttggcgac gctttttatg
    16441 ttactaagag gtaccccttt tatttatcaa ggacaagaac taggaatgac caacatagaa
    16501 atggcatcat tagaagattt tgatgatgta gctacgcatt ctcagtataa acgggcatta
    16561 gaatatggat tggcaccaga gcaagcattg aaagctgtca ataaacgaag tcgggataat
    16621 tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca gattctcaac ttctgataat
    16681 gtatggttaa aagtaaatcc taattatcca gaactcaatg cagaagctca gttaaccaat
    16741 caacagtcgg tattcaatta ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac
    16801 aaagacgtgc taatctacgg acaatttcta ccagtgaaag ataaaaatga acatattttg
    16861 ctttatgaac gccaattaga agataaaatc gtattagtta tcttaaacgt tacagcaaca
    16921 gaacaagaat attatgtaga tgaacagtac caacaagtat taatcaataa ttatgaagat
    16981 gttttattag atcaaagtcg aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct
    17041 aattatggag gacaattatg aaaattaaaa acgaagcaat gctcatcacc tatgcagata
    17101 gtttaggaaa aaacatgggc gaattaaaac aagtcttaga caaacattta caaggtgtta
    17161 ttggtggtgt acatctgttg cctttttttc catcgacagg tgatcgaggc tttgcgccga
    17221 gtgattatac gacagttgat ccagctcttg gaacatggga agatgtggaa gcattaggtg
    17281 aagattatta tttgatgttt gattttatga ttaatcacat ttctcgtgaa tcaaaatttt
    17341 tccaagattt caaaaaaaac catgagcagt caccatacaa agaaatgttt atccgtattc
    17401 atgaattttt cccagaaaat cgaccaacac aagaagatat tgatttaatc tacaagcgta
    17461 aagacaaagc gccgtttcaa acggtagaat ttgcggatgg tacgacagaa gaggtttgga
    17521 atacctttgg ggaagaacaa attgatttag atgtgaccaa agaagttgtg aaagaattta
    17581 ttcgagaaac catcaaagat atggcgaacc atggctgttc tttaattcga ttagatgcgt
    17641 ttgcttatgc gattaaaaaa ttagatacca atgatttctt cgtggaacca gatatttggg
    17701 aacttttaga cgaagtgcgt caagaagcag cgaaatatga cgtcgaatta ttaccagaaa
    17761 tccatgagca ttattccatt caaatgaaaa ttgccaatca tgattattat atctatgact
    17821 tcgccttgcc aatggtcact ctgtattcac tttatagtgg aaaatcagaa cgtttagcca
    17881 attggttgaa aatgagtccg atgaagcagt ttacaacatt ggatacgcat gatggaattg
    17941 gtgttgtgga cgctcgtgat ttattaacag atgaagaatt ggattacacc tctgaggaat
    18001 tgtataaagt cggggcgaat gtgaagaagg tctattcaag tgccaattac aataatttag
    18061 atatttatca aattaacagc acctattata gtgcattagg cgataatgac cgtagctatc
    18121 tgttagcacg tgcgattcaa gtatttgcgc cagggattcc acaaatttat tacgttgggt
    18181 tattagcagg aaaaaatgat attgaactgt tggaagcaac gaaagaaggg cgtaatatta
    18241 atcgtcatta ctatgattta gccgaaattg aagaagaagt gcaacggcca gtgattcaac
    18301 aattatttgc acttttaaaa tttagaaatg aatctgcggc gtttgatttg gatggaacga
    18361 ttgaagtagc aacgccatca gaatcagaaa tagtgattac acgtaaaaat aaaaacggta
    18421 gtgatagtgc aacattaacg gctaacttgg atacgaaaga atttatcatt gagtctaaag
    18481 atataaaaat tttcactagt gttgcattaa aattttaacc tattccatag aacaaaaaat
    18541 cctttatact agcacttgga tgacttatcc aagaccaata aaaaggactc atacatggat
    18601 aagtataaaa caaatacagc atttaataaa tggttttctg ccataaaatt aggaaaactt
    18661 tctgtcaact cccaaaaatg tatttttgat tacaattcgt atcgtaaaaa actgtctttt
    18721 gaagctgtgc tcaagctctt tctctacgca gttaatgaag aaaaagaaag tttgcgtgat
    18781 ttgagtacag ctcttatcga ccgatcgctc caactagaaa ccgatgtgtc atcgattagt
    18841 catacccaat tgtctcgagc atttaatgca cttgaaccta aagtgttaga agaaattttt
    18901 caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat
    18961 ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt
    19021 caaacaaaat caggtgtcaa gttacactta aactattgtt atatggacga tactacgatg
    19081 tatccaactg attttacctt aacaaatgcc catgaacatg atctcaatca actaccagta
    19141 ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg ggtattagga ctttgaaaag
    19201 atggatcaga tgcattggga tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa
    19261 gttcacgtga tagatactct ggaaacatcg ccagaaacag aaattctacg agacgagctg
    19321 gttcgtttag gatctaaaac ctatctaacc gccaatttcc gactagtcac tgttcaagat
    19381 aagaacggac gtgtgtttca atttatcacc aatcgaatgg acgtctcttc aaaagaaatc
    19441 tctgatatgt atcacgctcg ttggcaaatc gaattgtttt tcaagcacat caaacaacac
    19501 atgaccatta aaaccttctt ttctcaaagt gaaaaaggcg tgcagaatca attgatttta
    19561 acgatgattt ccgctctcct cacgtttctc atcaaattgg agacgaaaac ggaaaaatca
    19621 gtgttccaaa tcaaacgatt ctttcgttat ttactgtttc agccctttga atgttgtcta
    19681 gaaaaattaa ttcccacatg agaaaaaaga aatttggctc gttacttttg gttttcagtt
    19741 gaataacaga taaaaaattt tctggaaaaa gtcatcctct gcgtcagcac agtttttttt
    19801 gacttatttt cagaggaaat agttttcaga atattctaaa atgttttatg caacactagt
    19861 gatatttttt agaattagat ttctaatgtg cattttttga gtattaagga gcaagtatac
    19921 aaagatttat aaagctgatt aaatttgaat aaaaatatgc aggaacgcta ctatttttag
    19981 tagtgttctt ttttatattt gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa
    20041 aatgaattaa tgaaattagg ttatgggaag tatcagtctg agagaatcat acgacaggca
    20101 aaagcaatta tggtttcaaa aagctatacc tattacgaaa atagaagatt aggactagta
    20161 cctgttgaat ctgtagaaga agttttaggt atgaagatag aggtagagga ggattttcag
    20221 aatcatggtt aattttaaaa aagataaaaa tgggaaatat cattttactg ctagtttggg
    20281 attcgacaaa cgaacaggga aaagagtaca aaaaaacgta gaggggttaa aacagttaaa
    20341 gaagaaaaaa agcgtatacc gaacttgttt ctaattatgg aaaagaagca gaaacttctc
    20401 aaagtactat gttgtttgag gagttttttt ataaattatt cctaccttat tatcaaggga
    20461 aagtagaaga aagaacatat aaaaatcggt tatctacaat caaaaaaact tttcttattt
    20521 ctttaaaatg gagttaagag aaatacgtcc aattgacatt caaaagtggc aaaataattt
    20581 attgagcaaa tataaaaatt cttatgtaag aaacatgtat gggatttttc agatgtcatt
    20641 agatcgagca gttgttttgg gactacttga aaaaaatcct gcaaaagtta ttggaaatgt
    20701 gaagaaaagg aaaactacag ttgatttttg gacaaaagaa gaatttacaa aagttatttc
    20761 aactttttat attgatgatt tgtaccaaca ttttggtttt atttgtatgt ggttactttt
    20821 tatgaccgtt atgcgtttgg gagaagccac tgcattaact tggaaagata ttgattttga
    20881 aaataaaaac ttgcgtgtga ataaaacact ctattataag agagccaaaa cctatgagtt
    20941 tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa ctagacgaca atacaattac
    21001 ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc
    21061 ttataatgga acacctactc agaaatatac aatccgtcat attatgagaa gacatgcaac
    21121 gatcgttggt gttcatataa tccgcataca cggattaagg catagtcatg cttctttgct
    21181 tatatcacaa taaaaagatg cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca
    21241 aacgacttta ggaacttatg gtcatttgta tctaaattca ttgagagaga taacaaatga
    21301 tcccacagat tatatccaaa ctaatttttc taaaatcaaa ccgacaccta tttacgataa
    21361 taaaaaataa atgataaaca aaagtaggat cacaccaaaa gaaacacact agaaaaacta
    21421 taatacaggc ttttctagcg tgttgtttct tatttccgcc tatttataca ttatactcat
    21481 aaagcattta atgcatcgat cgtagccgca ttgtctatat gggtatataa agttgttgta
    21541 ttggtacttg aatgtcccaa ttgttgggca gttaatacca atgaatttgt ctgttggtag
    21601 agtctagtag ctaatgtatg acgcagtttg tgagggctga cttgaattct aaagcctttt
    21661 gaatattttg ctaccatccg ttcgattgca ataccggata tacgcttggc ttctccttgg
    21721 taaattgcta gaaataaagc tttttcgttt tctgttactt tatatcgttc tttccgaata
    21781 gctagataat tagctaaata ttccattgcg aatgctgcta tattgacagc atctctttta
    21841 tttccttttc tagttactac cacattattc ttatctaaag ataggtcatc taaattaata
    21901 tttataacct cggagagtct caatccagat cctaaaatca gtgcattgat cgccaggtct
    21961 ctttctacat ctcgttgata atagatcaaa gctcgtttac tcaaagtatt tttatattca
    22021 ttggcaatat aatctagata attttgtgta tccttatcta gaaataattt tttctcaatg
    22081 gcattggcac gtgcagctaa cgtagtttta tctgtgatgg ttgctacttg atccatagga
    22141 ttattgtcta agtatggttt tcctagagga ttattggaac ttttcgaaag atatttaaat
    22201 agaaccttta atgccgtgat atttctattg atagtagaag tagatagacc taagttatta
    22261 ttatctttga gcttagtttt tgttgtctct ttttttacat tttcttttgt gcgacgtttc
    22321 aaatgagatt tgtacaagtt taggtcatag gcagttaact gtcttatcga ggtgatagaa
    22381 gtatcaggaa cgatagactc actcagaagc cattcataaa agagcttata attttttaga
    22441 tattctaaag tggttcgagg tgattgatct ttatcctcac gatcttgaac aaaatctttt
    22501 atataatcgg gcatatgaag tatgattgta tctatttttt gatatatttc tgtatttttc
    22561 atgtcattca tcctttcaat aagtagggga gaagagtatc tctataattg gactatacca
    22621 attatagaat gttttgtcaa ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag
    22681 catataaaca ggtatacgtt taataattta aaataaatgg tatacataaa tagggaagga
    22741 aatagaaggg agcaatctaa attttttgaa ttttcttaat taattatata gaaagaacaa
    22801 tctttctata gagttagaca tgagagggac tatgcttgat ttaaaatcaa caagcgtata
    22861 tactgtatat acaaagaaaa aggagggaga agactatggc aattaaagaa agaaaattta
    22921 gggaagtggg gaactcagta gtattaacta tttcaaagga gtttcttaaa gaaaatgggt
    22981 taaatcccgg agatacggtt tttttagatg aagaaaaact tcgagcggca attacaaaaa
    23041 aacaagcctt atctgatgaa attgatttag caatatctca agcattaact gaatatgata
    23101 ctacttttag agagcttgtc gataaatgag tattcattac ctagaagcca aagatattat
    23161 aaaaatgaat gtaatccaaa taaaaaaata ctctcctaat gaaccccttg gtgtaaagga
    23221 tgcgaatgct ttagaaatgt gtgtagggca acttcgagcc tcagcttttg gtgaagaggt
    23281 ttatccttcg atttatgaaa aagcagctat cctacttata caattgataa agaaacatcc
    23341 ttttcacaat gcaaataaac gtactgcttt cttagctact tttgtctttt tgaaaatcaa
    23401 tggacagctt ttagctattg atcaaaaaga tgctgtaaat ttagcggtat atattgctac
    23461 gtatgataag gatttcgatc aactaaaata tgaagtgatc aacactataa agacacatac
    23521 gcagcccatt taaggagtta ctgtatgtgt ctttataggg ttattagctg ctatagcgaa
    23581 ataattgaag taaattacaa atagctataa tt
//
LOCUS       DAYYHB010000041        22094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000041 DAYYHB010000000
VERSION     DAYYHB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.042"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..3398
                     /locus_tag="ACHA6B_001544"
     CDS             120..3398
                     /locus_tag="ACHA6B_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8949118.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3939..5846)
                     /locus_tag="ACHA6B_001545"
     CDS             complement(3939..5846)
                     /locus_tag="ACHA6B_001545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8949119.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5976..6851
                     /locus_tag="ACHA6B_001546"
     CDS             5976..6851
                     /locus_tag="ACHA6B_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8949120.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6931..7905
                     /locus_tag="ACHA6B_001547"
     CDS             6931..7905
                     /locus_tag="ACHA6B_001547"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8949121.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8085..9230
                     /locus_tag="ACHA6B_001548"
     CDS             8085..9230
                     /locus_tag="ACHA6B_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8949122.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9367..9711
                     /locus_tag="ACHA6B_001549"
     CDS             9367..9711
                     /locus_tag="ACHA6B_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8949123.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9982..11268
                     /gene="efmA"
                     /locus_tag="ACHA6B_001550"
     CDS             9982..11268
                     /gene="efmA"
                     /locus_tag="ACHA6B_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8949124.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11406..12005)
                     /locus_tag="ACHA6B_001551"
     CDS             complement(11406..12005)
                     /locus_tag="ACHA6B_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8949125.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12409..13530
                     /gene="mnmA"
                     /locus_tag="ACHA6B_001552"
     CDS             12409..13530
                     /gene="mnmA"
                     /locus_tag="ACHA6B_001552"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8949126.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13776..15197
                     /gene="arcD"
                     /locus_tag="ACHA6B_001553"
     CDS             13776..15197
                     /gene="arcD"
                     /locus_tag="ACHA6B_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8949127.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15211..16329
                     /locus_tag="ACHA6B_001554"
     CDS             15211..16329
                     /locus_tag="ACHA6B_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8949128.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16389..17681)
                     /locus_tag="ACHA6B_001555"
     CDS             complement(16389..17681)
                     /locus_tag="ACHA6B_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8949129.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17831..18391
                     /locus_tag="ACHA6B_001556"
     CDS             17831..18391
                     /locus_tag="ACHA6B_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8949130.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18520..18897
                     /locus_tag="ACHA6B_001557"
     CDS             18520..18897
                     /locus_tag="ACHA6B_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8949131.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18830..20902
                     /locus_tag="ACHA6B_001558"
     CDS             18830..20902
                     /locus_tag="ACHA6B_001558"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8949132.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21113..22060)
                     /locus_tag="ACHA6B_001559"
     CDS             complement(21113..22060)
                     /locus_tag="ACHA6B_001559"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8949133.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7142 a         4040 c         4685 g         6227 t
ORIGIN      
        1 gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta ttgacagtaa
       61 aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact
      121 tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc
      181 cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa
      241 atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag
      301 atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa
      361 aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa
      421 gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata
      481 acggcggaaa attccagtca agcggatcag atggaatgac ctattattat caagcaatcc
      541 ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga
      601 atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg
      661 ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg
      721 actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat
      781 tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg
      841 ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa
      901 caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga
      961 ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg
     1021 atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg
     1081 cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg
     1141 tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac
     1201 ctactcgctt gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag
     1261 atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa
     1321 caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt
     1381 atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa
     1441 actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata
     1501 cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg
     1561 caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg
     1621 ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc
     1681 caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag
     1741 aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc
     1801 actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac
     1861 agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag
     1921 gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag
     1981 tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg
     2041 cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg
     2101 cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca
     2161 tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg
     2221 gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt
     2281 tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca
     2341 atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag
     2401 gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga
     2461 gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact
     2521 attggaatga acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt
     2581 ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa
     2641 atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa
     2701 aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac
     2761 cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg
     2821 aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa
     2881 atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa
     2941 cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt
     3001 caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata
     3061 cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag
     3121 aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa
     3181 atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa
     3241 aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc
     3301 caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg
     3361 gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat
     3421 gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac
     3481 ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc tgttcttatt
     3541 acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa tcagctgacc
     3601 ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt tttttctcta
     3661 atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca aaagccagtt
     3721 tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     3781 tactacttga aactaagcag ctttttcaca acctctaata catggtattc cagaagtaac
     3841 ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc ccaaattcct
     3901 tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg cactcttttt
     3961 tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata gaattcgtca
     4021 aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc gatttgcagt
     4081 aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca attgacaaag
     4141 aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca gcttagaccg
     4201 cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc gtcagcaaaa
     4261 ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg tgtaagatct
     4321 tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc gttattatca
     4381 aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt agcagagctt
     4441 gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac gatcacattc
     4501 cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc tgaacgatcc
     4561 actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa aacaagagta
     4621 tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc gaataatgct
     4681 gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat gacggctttt
     4741 tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata aacggcgatc
     4801 atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata tttttttccg
     4861 tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc tgcttttttc
     4921 tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct gacttggcgg
     4981 aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat ccagcttttg
     5041 aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta cactggatca
     5101 gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt tttcaagtcc
     5161 agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat tttgctgtaa
     5221 tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa ttgcactgct
     5281 tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa taactgaaca
     5341 gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga tgcataatac
     5401 gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt ttgcgcattc
     5461 ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa cagccattct
     5521 ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc gattataggt
     5581 tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag ttgataatat
     5641 gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag cagatcgtct
     5701 gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc gcctgtagct
     5761 ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc ttcatagaaa
     5821 tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt cgtgtgccca
     5881 ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa ttcgaacaat
     5941 caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg cgatcccaac
     6001 tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag
     6061 ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg
     6121 aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa
     6181 aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa
     6241 tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc gaagaaaaaa
     6301 ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt
     6361 ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc
     6421 ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt cgcaagaaaa
     6481 atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac
     6541 ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca
     6601 atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac
     6661 gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc
     6721 attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac
     6781 acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt
     6841 cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc
     6901 gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg
     6961 aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga
     7021 acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc
     7081 gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat
     7141 gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg
     7201 atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat
     7261 gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta
     7321 ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat
     7381 ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac
     7441 atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat
     7501 ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat
     7561 gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga
     7621 gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc
     7681 tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc
     7741 aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta
     7801 acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca tgaaaataca
     7861 ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg
     7921 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
     7981 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
     8041 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
     8101 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
     8161 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
     8221 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
     8281 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
     8341 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
     8401 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
     8461 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
     8521 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
     8581 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
     8641 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
     8701 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
     8761 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
     8821 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
     8881 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     8941 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     9001 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     9061 gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca atgtatggag
     9121 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     9181 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     9241 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     9301 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     9361 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     9421 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     9481 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     9541 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     9601 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     9661 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     9721 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     9781 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     9841 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     9901 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     9961 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
    10021 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
    10081 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
    10141 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
    10201 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
    10261 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
    10321 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
    10381 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
    10441 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
    10501 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
    10561 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
    10621 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
    10681 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
    10741 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
    10801 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
    10861 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
    10921 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
    10981 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
    11041 cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg attattattt
    11101 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
    11161 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
    11221 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
    11281 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
    11341 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
    11401 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
    11461 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
    11521 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
    11581 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
    11641 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
    11701 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
    11761 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
    11821 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
    11881 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
    11941 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
    12001 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
    12061 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
    12121 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
    12181 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
    12241 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
    12301 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
    12361 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
    12421 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
    12481 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
    12541 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
    12601 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
    12661 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
    12721 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
    12781 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
    12841 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
    12901 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
    12961 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
    13021 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
    13081 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
    13141 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
    13201 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
    13261 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
    13321 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
    13381 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
    13441 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
    13501 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
    13561 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
    13621 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
    13681 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
    13741 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
    13801 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
    13861 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
    13921 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
    13981 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
    14041 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
    14101 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
    14161 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
    14221 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
    14281 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
    14341 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
    14401 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
    14461 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
    14521 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
    14581 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
    14641 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
    14701 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
    14761 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
    14821 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
    14881 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
    14941 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
    15001 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
    15061 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
    15121 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
    15181 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
    15241 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
    15301 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
    15361 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
    15421 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
    15481 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
    15541 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
    15601 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
    15661 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
    15721 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
    15781 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
    15841 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
    15901 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
    15961 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
    16021 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
    16081 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
    16141 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
    16201 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
    16261 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
    16321 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
    16381 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
    16441 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
    16501 ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa tgcactggaa
    16561 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
    16621 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
    16681 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
    16741 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
    16801 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
    16861 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
    16921 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
    16981 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
    17041 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
    17101 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
    17161 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
    17221 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
    17281 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
    17341 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
    17401 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
    17461 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
    17521 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
    17581 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
    17641 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
    17701 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
    17761 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
    17821 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
    17881 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    17941 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    18001 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    18061 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    18121 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    18181 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    18241 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    18301 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    18361 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    18421 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    18481 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    18541 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    18601 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    18661 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    18721 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    18781 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    18841 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    18901 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    18961 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    19021 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    19081 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    19141 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    19201 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    19261 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    19321 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    19381 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    19441 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    19501 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    19561 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    19621 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    19681 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    19741 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    19801 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    19861 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    19921 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    19981 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    20041 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    20101 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    20161 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    20221 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    20281 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    20341 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    20401 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    20461 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    20521 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    20581 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    20641 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    20701 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    20761 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    20821 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    20881 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    20941 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    21001 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    21061 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    21121 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    21181 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    21241 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    21301 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    21361 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    21421 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    21481 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    21541 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    21601 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    21661 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    21721 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    21781 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    21841 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    21901 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    21961 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    22021 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    22081 tatgtttata tatg
//
LOCUS       DAYYHB010000042        20994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000042 DAYYHB010000000
VERSION     DAYYHB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.043"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..986)
                     /locus_tag="ACHA6B_001560"
     CDS             complement(111..986)
                     /locus_tag="ACHA6B_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8949134.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1068..1859)
                     /gene="codY"
                     /locus_tag="ACHA6B_001561"
     CDS             complement(1068..1859)
                     /gene="codY"
                     /locus_tag="ACHA6B_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8949135.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1877..3277)
                     /gene="hslU"
                     /locus_tag="ACHA6B_001562"
     CDS             complement(1877..3277)
                     /gene="hslU"
                     /locus_tag="ACHA6B_001562"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8949136.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3291..3839)
                     /gene="hslV"
                     /locus_tag="ACHA6B_001563"
     CDS             complement(3291..3839)
                     /gene="hslV"
                     /locus_tag="ACHA6B_001563"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8949137.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3876..4781)
                     /gene="xerC"
                     /locus_tag="ACHA6B_001564"
     CDS             complement(3876..4781)
                     /gene="xerC"
                     /locus_tag="ACHA6B_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8949138.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4979..6355)
                     /gene="trmFO"
                     /locus_tag="ACHA6B_001565"
     CDS             complement(4979..6355)
                     /gene="trmFO"
                     /locus_tag="ACHA6B_001565"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8949139.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6324..8402)
                     /gene="topA"
                     /locus_tag="ACHA6B_001566"
     CDS             complement(6324..8402)
                     /gene="topA"
                     /locus_tag="ACHA6B_001566"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8949140.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8524..9381)
                     /gene="dprA"
                     /locus_tag="ACHA6B_001567"
     CDS             complement(8524..9381)
                     /gene="dprA"
                     /locus_tag="ACHA6B_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8949141.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9437..10204)
                     /locus_tag="ACHA6B_001568"
     CDS             complement(9437..10204)
                     /locus_tag="ACHA6B_001568"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8949142.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10197..11057)
                     /gene="ylqF"
                     /locus_tag="ACHA6B_001569"
     CDS             complement(10197..11057)
                     /gene="ylqF"
                     /locus_tag="ACHA6B_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8949143.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11317..11715
                     /locus_tag="ACHA6B_001570"
     CDS             11317..11715
                     /locus_tag="ACHA6B_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8949144.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11952..12494
                     /locus_tag="ACHA6B_001571"
     CDS             11952..12494
                     /locus_tag="ACHA6B_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8949145.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12577..13128)
                     /gene="lepB"
                     /locus_tag="ACHA6B_001572"
     CDS             complement(12577..13128)
                     /gene="lepB"
                     /locus_tag="ACHA6B_001572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8949146.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13280..13507)
                     /locus_tag="ACHA6B_001573"
     CDS             complement(13280..13507)
                     /locus_tag="ACHA6B_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8949147.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13504..14022)
                     /gene="msrA"
                     /locus_tag="ACHA6B_001574"
     CDS             complement(13504..14022)
                     /gene="msrA"
                     /locus_tag="ACHA6B_001574"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8949148.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14042..14755)
                     /locus_tag="ACHA6B_001575"
     CDS             complement(14042..14755)
                     /locus_tag="ACHA6B_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8949149.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14758..15654)
                     /locus_tag="ACHA6B_001576"
     CDS             complement(14758..15654)
                     /locus_tag="ACHA6B_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8949150.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(15812..16654)
                     /locus_tag="ACHA6B_001577"
     CDS             complement(15812..16654)
                     /locus_tag="ACHA6B_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8949151.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16786..17439
                     /locus_tag="ACHA6B_001578"
     CDS             16786..17439
                     /locus_tag="ACHA6B_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8949152.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(17502..18029)
                     /locus_tag="ACHA6B_001579"
     CDS             complement(17502..18029)
                     /locus_tag="ACHA6B_001579"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8949153.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(18049..18996)
                     /locus_tag="ACHA6B_001580"
     CDS             complement(18049..18996)
                     /locus_tag="ACHA6B_001580"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8949154.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(19008..20891)
                     /locus_tag="ACHA6B_001581"
     CDS             complement(19008..20891)
                     /locus_tag="ACHA6B_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8949155.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         5551 a         4411 c         3665 g         7367 t
ORIGIN      
        1 taaaacggga gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt
       61 tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact
      121 gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt
      181 tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa
      241 acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag
      301 cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt
      361 gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt
      421 gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat
      481 cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga
      541 ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt
      601 gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca
      661 aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt
      721 tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt
      781 tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat
      841 aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca
      901 tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc
      961 tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa
     1021 tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt
     1081 tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct
     1141 aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca
     1201 gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca
     1261 tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt
     1321 gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa
     1381 aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca
     1441 aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc
     1501 ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta
     1561 atatcactag taattgggat attagcttca gttttcatca actgtgccac tgtatcagta
     1621 taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg
     1681 tgacgttcat tataacctag taatactccg tcacttccta tgatataggt gttgctgtca
     1741 agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa
     1801 gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt
     1861 gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc
     1921 caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg
     1981 cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga
     2041 tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt
     2101 ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag
     2161 cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta
     2221 cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga
     2281 aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta
     2341 cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc
     2401 cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt
     2461 cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt
     2521 cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct
     2581 tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt
     2641 tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca
     2701 gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca
     2761 cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt
     2821 caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca
     2881 aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta
     2941 cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat
     3001 aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg
     3061 ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt
     3121 ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca
     3181 aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac
     3241 ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct
     3301 tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt
     3361 tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca
     3421 gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact
     3481 tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca
     3541 agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt
     3601 agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct
     3661 gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt
     3721 gcagtgcctt tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca
     3781 aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg
     3841 tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
     3901 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
     3961 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
     4021 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
     4081 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
     4141 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
     4201 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
     4261 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
     4321 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
     4381 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
     4441 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
     4501 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
     4561 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
     4621 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
     4681 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
     4741 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
     4801 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
     4861 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
     4921 aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt
     4981 actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag
     5041 ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag
     5101 gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt
     5161 agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg
     5221 ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt
     5281 ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg
     5341 gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga
     5401 attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca
     5461 agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct
     5521 ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag
     5581 gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt
     5641 caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct
     5701 catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt
     5761 tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg
     5821 gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg
     5881 ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag
     5941 taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt
     6001 cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg
     6061 ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat
     6121 tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt
     6181 gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt
     6241 gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt
     6301 tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca
     6361 cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga
     6421 caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa
     6481 ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact
     6541 gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta
     6601 ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca
     6661 tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca
     6721 ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc
     6781 gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg
     6841 atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca
     6901 aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca
     6961 taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta
     7021 aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca
     7081 tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct
     7141 tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg
     7201 acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta
     7261 gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt
     7321 ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt
     7381 gcgccttgag catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta
     7441 tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta
     7501 cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat
     7561 tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt
     7621 tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact
     7681 tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca
     7741 tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga
     7801 gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct
     7861 ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct
     7921 ttcttgacct tacgccagag aataggactg atcgaataac caacgattct gtctaaggta
     7981 cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct
     8041 gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc
     8101 aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt
     8161 gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct
     8221 tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat acccatttta
     8281 cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga
     8341 tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc
     8401 attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa
     8461 atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg
     8521 gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc
     8581 ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa
     8641 aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt
     8701 ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa
     8761 acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc
     8821 aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat
     8881 gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc
     8941 tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata
     9001 aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca
     9061 agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag
     9121 cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt
     9181 gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat
     9241 tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca
     9301 gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa
     9361 attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg
     9421 taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga
     9481 ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat
     9541 tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat
     9601 atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtacatcc cctttgatta
     9661 ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta
     9721 caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca taaatattca
     9781 catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa
     9841 cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta
     9901 atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc
     9961 ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt
    10021 caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt
    10081 caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg
    10141 taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag
    10201 tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct
    10261 tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt
    10321 tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa
    10381 ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc
    10441 gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg
    10501 ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac
    10561 tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt
    10621 gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct
    10681 aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt
    10741 tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc
    10801 gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt
    10861 tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc
    10921 tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg
    10981 atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga
    11041 aaccattgga tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta
    11101 cttgtttttt tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt
    11161 agttaagtga aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa
    11221 atttttattt tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag
    11281 atgatagacg aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat
    11341 tttcctgttg ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa
    11401 aatgaagaat tttctactga agaagctggt tcaatggtat gggatgacac tttttggata
    11461 atgcttctct ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat
    11521 aaaaaaacaa gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa
    11581 tttgctcaaa ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct
    11641 gaagaagaaa tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta
    11701 tggatgaaga tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg
    11761 gatgatacga taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca
    11821 gtttataaaa gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc
    11881 accaatgtgt catgacacat tggtgatttt atggtatcct tttattatta actgacaaag
    11941 gagagaatat tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg
    12001 caagattgtt gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag
    12061 cgattggtcc tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt
    12121 atctatatgg accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca
    12181 cgatatttgg ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat
    12241 ttattaatgg cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc
    12301 aattattgat tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt
    12361 ttggtgcaga tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc
    12421 ctattcaaat aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta
    12481 aacgagctgt ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag
    12541 cgtgcagtct caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc
    12601 aagtggataa tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa
    12661 cgaacgacta tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg
    12721 aagcttggat tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc
    12781 atgatctgat ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt
    12841 caattgatca ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata
    12901 aatcgtgcca tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt
    12961 ttccatcaca accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg
    13021 taccgggatg aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta
    13081 tttgaataca agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc
    13141 catccctctt tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag
    13201 atagctgaaa taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca
    13261 taaagtacaa aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt
    13321 cgtcaaagat atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt
    13381 cttcgtagct agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa
    13441 cagataaagc atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc
    13501 gattcaccct tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct
    13561 tttaaaattt tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa
    13621 tggttgggct ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa
    13681 ctgtttctta cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg
    13741 gcgataattg tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg
    13801 ccagtaaagt tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac
    13861 tgcttctgta tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg
    13921 ccctccagta tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat
    13981 catacaccag aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat
    14041 actactcatc cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag
    14101 ttttgttggt atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa
    14161 accgaatgtc atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca
    14221 ttcggaattg atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat
    14281 ctggtaattt atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg
    14341 ttccgataga aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat
    14401 caaaatataa atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg
    14461 cttcattttc caaataaaat ttatattcaa tatcactatc tttttgatac tcactaagga
    14521 aaaagctgat gatttgattg actttttctt tatttgaatt gatcgttaag acaggatctc
    14581 cttgtctttc cgtaagagct ggtatttctg ggagttctgg ttcccgatta gcaaaaatac
    14641 ggctgcctaa aaatacggtc gaaccaagta ctaccccaag aaggataagg aaggcgaatt
    14701 tccagtaatt tgttttcttt gttttgtttg aagatacgtc agatcttgtt ttcatgttta
    14761 ttgatcatcc tccttctcta accattggtt ttgtgtctcg atcagctgat ctctcactgc
    14821 atttgccatc aattgataac ctaaattatt tggatggaaa tgatcttctt catagagcac
    14881 attatttttg atgccattta gattttctgt cgtgctagtt gtttgatctg ttccttcttc
    14941 tgtgacacct acttcctgat tcaatccttg ataaagcaaa tcattgattg ggataaagta
    15001 gcagttcggc attttttttg tcaccgtttc tgttcctttg ttccaattgt taacaattgt
    15061 ctgcatgtca gtgatctcag gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta
    15121 aataggtgcg gttttattga gggatcgaaa ttcttccaat agttctgtga cattttcctg
    15181 atagtttttc agtggctttt taaaggaatt gaccgttaac ccgaataaat tattttggat
    15241 aactttcatc aaatcattac cgccaacagt cagtgtgata aaatcagctg ttttgatatt
    15301 gtttcgaatt tcttcttttg tttttactcg ctttaagatc tggtcacttc gttcaccatt
    15361 tacaccatag ttttctgttt caaccgcggt caaattataa cggtctttta gatcatttgc
    15421 gacaagggga acgaatcctc cttgcttggt ttgatctcct actccttcag tcagcgaatc
    15481 tccgatagct atgtagtgaa gtttttcttt tttatttgga tcttctctca cagtagttac
    15541 tggtttcatt agtgggccag ctttcggtat agaagcagct aaacctgcat agatgatgat
    15601 cgcaattaat aatgggaaca aaacgaggat caaatgtcgt ttcgttaatt tcattttttc
    15661 acctcttgta ttcgattggt tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta
    15721 tgaaaaaaga aaacaattat tttcctaatt ttataaagaa aaagagaata agctgcaagc
    15781 ctttcaggct gagggcttat cctcttttcg actaatctac ataatacatg attgcaaacg
    15841 cgtctttacc agtatgcgta gcgataaccg gattcgtatg cagtacaggg atatgcatgt
    15901 ccgggaataa ttcttgcaaa ccctctttga attcttgcgc caattctttt gcatccgcat
    15961 gtgaaatacc gatttgtcgc acatttggca attctttcag ttcatttttc aaattattaa
    16021 accatttatc gaacgttttc tttcctcgcc ccttagttac agggattaat tcagaatggg
    16081 cgaaattcat caccacgcgt atgttcaaaa cactagacag caaaccagtt gttcggctga
    16141 ttcttccacc ttttaccaag ttatctagtg tggatattcc gatgtataat tttgtattgt
    16201 cttgtacttt tttgacttct tcaaggattt tctctactgt tttaccttct tttgctaatt
    16261 cagctgctcg aattacctga aaagataatc cttggtcagt ggtatcactg tcgataaccg
    16321 ttactttcgt ttttgttaga ttactggctt gtcttgctgc ttctactgtt ccactcaagc
    16381 cttttgtcat atggatcgaa atcacttcac tgccgtcttc tcctagctca tcatacatcg
    16441 agatgaattc tccaattggt ggttggcttg ttttgggcag attttttgct ttcgccatca
    16501 tttccataaa tctttcgccg tctagttggt catcatcggg atatactgtt ccgtcgatca
    16561 tgatggatag cggcatgaca cgaatgccta aagcatcgcg tacatttttt tccatcgtac
    16621 atgaagaatc tgttacgatt tttacatttg tcattatttt ctctctctct ttctttaaca
    16681 agatgcctta ttcatgttaa actacatgta agacaatttc tatttttagt ataacagaca
    16741 aataatgatt tttcattaat tacacctagt aaaggagccg ttttcatgga aaaaacgaaa
    16801 tttaatcgaa cgtatgaaat attaaatgaa gtgtttaatg cagtcaccca tggaattggg
    16861 actggattga gtatcgcggg actagtggtt cttttagtaa aaggtgccca cttgcattcc
    16921 tccctccata ttgtctctta tgctatctat ggatcgatga tgattctttt atttttaacg
    16981 tctactttgt ttcattcatt gatttttaca aaagcaaaaa aggtgttcca agtattcgat
    17041 catagttcga tttttctttt gattgcagga agttatactc ctttttgttt gctgagtatc
    17101 cgaggatggc taggttggac tttatttgta ttaatctggt tgtttgcgat tcttggcatt
    17161 atatataaag cgttgacgtt gcataaacgg gagagagtat caaaagtatc tacgatcatc
    17221 tatattttta tggggtggct ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga
    17281 atgactggtg ttattttgat ggtagcaggc gggctttctt acacgatagg cgcattcttt
    17341 tatagcttga attctatccg ctatatgcat gttgtttggc atttgtttgt tatgttaggt
    17401 gcaggtttta tgtacttctc tgttttattc tttacttaat aaaaaaaata aagctatgga
    17461 tgggacagct ctaaataaca gctagtctat ccatagcttt tttatatcgt caattcgcct
    17521 gtcccgcttt ttacaggatt ctcattacgc tgatacgttt caaattgatg cggataacga
    17581 tttttttcat ccatttctcc ttgactagta ttgatcagtg accaatcaga ccagtcaaca
    17641 ggtggaaaat aagcatctcc atcgaataca tgatggatga ctgtacggta taatatcgta
    17701 caaaatggca gaaattcttg ataaacagct gaccctccag cgatgaaaaa gataccgtct
    17761 gtttcttttt ccagagcaag cacgtcttcc acactatgca tgacatgcac gccttctgct
    17821 ttataggaag aatctctcgt caaaacaatc gtctgacgat tcggcaacgg acggctaccc
    17881 atcccttcga atgtttttcg tcccatcact agtgtatttg cttctgtcat ttgtttgaag
    17941 aatttcagat catttggcaa acgccaagga agacggtctt cttttccaat caatccgttt
    18001 tcatcctgtg cccaaattgc agctaacatt ttctcatcct ttctgattct aaacagcgat
    18061 aggagctttg atcgccggat gtgggtcata tccttctaat tggatatctt ccatctcaaa
    18121 atcaaatact gactgctttt cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg
    18181 gctcaattgt tctttcattt gttcgatatg attgatataa agatgagcat ctcctaatgt
    18241 gtgaacaaat tcgccaaccg ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc
    18301 ataacttgca atgttgaacg gaacgcctag aaacacatcc ccactgcgtt gatacaattg
    18361 gcagcttagc cgtccctctt gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc
    18421 catgctagga acatcttccg gattccatgc agaaacgatc aatcttctag aatcaggagt
    18481 ctttttgatt gcttcgatca cttgtttgat ttgatcgata aagccaccgt cttttgtttc
    18541 ccagtgacgc cattgatagc cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga
    18601 atcatcggtt aggatcttct cgcaaaaaag cttgctctgc tttttatatt cttcattgaa
    18661 ttcaggatct gtcaaacatc tatggccaaa atcagtcata tctggtccat tatagtctgg
    18721 acttttgatg tagcgttcaa atgcccattc atcccaaata tggtttttat tttgtaatag
    18781 atagcggata ttcgtatctc cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa
    18841 aggtactcgt ttcgttgtta acaaaggaaa ccctttttgc agatcaaacc gcatttggta
    18901 tccgaataag cttaatgtgc ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa
    18961 caattttctt cctaagtcaa gatacgcttg ttccatcctt tttcctccta atcttctacc
    19021 cattcactca agtattccca ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat
    19081 acgtcgtttt gcaattcttg taatcgagtg aaattatctc cttgatgatt catctcttct
    19141 tggagcaatg agattttttc ttcaagttct gcaatctcat cttcaatcgt ttcccattct
    19201 ttttgttcca tgtaagataa tttcttcttt tcagcaggtt ccttgctctg tatttttggt
    19261 ttcgcttttt cagcaggctg ctgggtctct ttttgcttca gtaaataact ggacatgttt
    19321 ccgtaataaa gttcttgttt tccgtttcct tggaatacta ataacttatc tgctaccttg
    19381 tccaagaaat aacggtcatg ggaaacagtg atcacagcac ctggaaaact tctaaaataa
    19441 tcttccaaaa tcgtcaatgt tgctatgtcc aaatcattgg tcggttcatc caataataaa
    19501 acattaggtt gctgaatcag cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa
    19561 agtttgccta tcagtgtccc atgcataaat cttggaaata aaaatcgttc aagtaattct
    19621 gctactccga tttgactgcc atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa
    19681 gcaatcatcc gtttatctgg tgccatttct tcattttctt gtgtataata cgcaagatga
    19741 accgtttcac cgatagacat tgtaccgcta tccaggggaa ttctgccagc tagaatatta
    19801 agcaatgtcg attttcccgc accattttta ccagtaatac ctagtctttc ccgagattga
    19861 atcaataaat ctaaatgttc tagcagtgtt tggtcattta tacggtaact gccatctttg
    19921 atttccagta cttttttccc taaacgttgt gtcgctaggt tcagctctaa atcatcttct
    19981 tggtttactt ggtacaagtt ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc
    20041 ttggtgccac gtgcctgtac gccagccctc atccaagcta attcctgttt ataaagccgc
    20101 ttgcgctttt cttcttgttc tacttctaca cgatcacgtt ctgctttttc taagacgtaa
    20161 gcttcataat ttcctttata ctcgtataat tttccaaatg aaagttcgaa gattcggttt
    20221 gctactctat ctagaaaata acgatcgtgg gtaaccatca atagtgcgcc acggtattgt
    20281 ttcaaatagt tttccaacca ctcgatcgct tcataatcta aatggtttgt tggttcgtct
    20341 agtaatagta aatcgggttc atcaatcaac acttgcgcca gactaactcg ttttttctga
    20401 ccacctgata aagtactgat ttttttatct aattcactga ttcctaattt ttgcaaaata
    20461 atctttgcgt tggtatctgt agtccaagca tcttctttat tcatttgttc ttctgcttta
    20521 gcataacgtt tttgcacatc agggtcttct ccgttttccc ctagttcgat caatgcttcc
    20581 tcatatgctc gaacagtttg gatcaagggg ctgtttcctt gaaagactgc ttggagaaca
    20641 gttaattcat cggaaaattc agctgcttga gaaagataac caatacggta atcagttgga
    20701 taaaacactg tttgccgatc accgtcgctg ctatcgatac cggcaataat atttaagaga
    20761 cttgttttac ccgtcccgtt cgtcccaatc aacccgatac ggtctttttc gtggaccaaa
    20821 aaagaaatct ggtcaaacaa atctttttca ccataagttt ttttgaggtc agttactttt
    20881 aattctttca acatgttccc tccttatctt ggatattgta tcataaaaat agtgaacttc
    20941 aggaaaattt ttttggtatt tgttcttttt gtgttctcaa aaaaagaaca ggac
//
LOCUS       DAYYHB010000043        20929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000043 DAYYHB010000000
VERSION     DAYYHB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.044"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACHA6B_001582"
     CDS             complement(<1..276)
                     /locus_tag="ACHA6B_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8949156.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLN"
     gene            complement(413..1699)
                     /locus_tag="ACHA6B_001583"
     CDS             complement(413..1699)
                     /locus_tag="ACHA6B_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8949157.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            1972..2550
                     /locus_tag="ACHA6B_001584"
     CDS             1972..2550
                     /locus_tag="ACHA6B_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8949158.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            2550..3479
                     /locus_tag="ACHA6B_001585"
     CDS             2550..3479
                     /locus_tag="ACHA6B_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949159.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            3476..5212
                     /locus_tag="ACHA6B_001586"
     CDS             3476..5212
                     /locus_tag="ACHA6B_001586"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8949160.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(5275..6111)
                     /gene="pheA"
                     /locus_tag="ACHA6B_001587"
     CDS             complement(5275..6111)
                     /gene="pheA"
                     /locus_tag="ACHA6B_001587"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8949161.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(6108..6617)
                     /locus_tag="ACHA6B_001588"
     CDS             complement(6108..6617)
                     /locus_tag="ACHA6B_001588"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8949162.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(6610..7905)
                     /gene="aroA"
                     /locus_tag="ACHA6B_001589"
     CDS             complement(6610..7905)
                     /gene="aroA"
                     /locus_tag="ACHA6B_001589"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8949163.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(7918..9009)
                     /locus_tag="ACHA6B_001590"
     CDS             complement(7918..9009)
                     /locus_tag="ACHA6B_001590"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8949164.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(9026..10192)
                     /gene="aroC"
                     /locus_tag="ACHA6B_001591"
     CDS             complement(9026..10192)
                     /gene="aroC"
                     /locus_tag="ACHA6B_001591"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8949165.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(10194..11258)
                     /gene="aroB"
                     /locus_tag="ACHA6B_001592"
     CDS             complement(10194..11258)
                     /gene="aroB"
                     /locus_tag="ACHA6B_001592"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8949166.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(11291..12313)
                     /gene="aroF"
                     /locus_tag="ACHA6B_001593"
     CDS             complement(11291..12313)
                     /gene="aroF"
                     /locus_tag="ACHA6B_001593"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8949167.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(12972..13946)
                     /locus_tag="ACHA6B_001594"
     CDS             complement(12972..13946)
                     /locus_tag="ACHA6B_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8949168.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(13927..15810)
                     /locus_tag="ACHA6B_001595"
     CDS             complement(13927..15810)
                     /locus_tag="ACHA6B_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8949169.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(15828..18053)
                     /locus_tag="ACHA6B_001596"
     CDS             complement(15828..18053)
                     /locus_tag="ACHA6B_001596"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8949170.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            18168..19202
                     /locus_tag="ACHA6B_001597"
     CDS             18168..19202
                     /locus_tag="ACHA6B_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949171.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(19269..20282)
                     /locus_tag="ACHA6B_001598"
     CDS             complement(19269..20282)
                     /locus_tag="ACHA6B_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949172.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(20360..20548)
                     /locus_tag="ACHA6B_001599"
     CDS             complement(20360..20548)
                     /locus_tag="ACHA6B_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8949173.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         5875 a         4532 c         3791 g         6731 t
ORIGIN      
        1 atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa
       61 atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata
      121 attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa
      181 agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa
      241 agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt
      301 gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg
      361 tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag
      421 ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat
      481 tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt
      541 aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac
      601 tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata
      661 ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt
      721 ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc
      781 cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa
      841 tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag
      901 ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg
      961 tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag
     1021 atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct
     1081 gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc
     1141 ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca
     1201 tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta
     1261 cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat
     1321 acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag
     1381 aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg
     1441 tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa
     1501 caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga
     1561 tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac
     1621 tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa
     1681 ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg
     1741 ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat
     1801 ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc
     1861 tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc
     1921 tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata
     1981 cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat
     2041 tggtctgagc acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat
     2101 ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat
     2161 ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc
     2221 tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat
     2281 cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa
     2341 aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag
     2401 aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa
     2461 cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta
     2521 aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc
     2581 ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac
     2641 ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt
     2701 ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca
     2761 aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt
     2821 tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact
     2881 acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg
     2941 atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg
     3001 ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat
     3061 tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac
     3121 tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg
     3181 agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc
     3241 atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa
     3301 aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta
     3361 ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt
     3421 ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag
     3481 aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct
     3541 ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat
     3601 ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag
     3661 tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag
     3721 ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca
     3781 aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt
     3841 tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc
     3901 tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct
     3961 ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa
     4021 aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa
     4081 gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat
     4141 gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat
     4201 gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg
     4261 tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc
     4321 taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc
     4381 tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga
     4441 ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca
     4501 tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta
     4561 tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc
     4621 acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca
     4681 gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac
     4741 gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta
     4801 catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga
     4861 tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta
     4921 tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa
     4981 tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa
     5041 acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct
     5101 aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt
     5161 aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc
     5221 caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct
     5281 acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag
     5341 catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc
     5401 gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa
     5461 gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc
     5521 aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg
     5581 gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa
     5641 gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt
     5701 cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac
     5761 attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag
     5821 gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat
     5881 ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc
     5941 tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc
     6001 gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca
     6061 agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg
     6121 atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga
     6181 tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct
     6241 cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact
     6301 agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga
     6361 ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc
     6421 tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat
     6481 tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac atactctttt
     6541 tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc
     6601 agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa
     6661 tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat
     6721 caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc
     6781 gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc
     6841 tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa
     6901 ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat
     6961 gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg
     7021 actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat
     7081 cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac
     7141 attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc
     7201 tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac
     7261 tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt
     7321 atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc
     7381 aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga
     7441 taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa
     7501 gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga
     7561 agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg
     7621 tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct
     7681 accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg
     7741 gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt
     7801 tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat
     7861 cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta
     7921 tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc
     7981 ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat
     8041 cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac
     8101 gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt
     8161 atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga
     8221 ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa
     8281 tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga
     8341 ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata
     8401 ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg
     8461 acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt
     8521 ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt
     8581 aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg
     8641 agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac
     8701 gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct
     8761 ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc
     8821 tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac
     8881 tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt
     8941 ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg
     9001 ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca
     9061 ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta
     9121 ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg
     9181 aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct
     9241 tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca
     9301 ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc
     9361 ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa
     9421 attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct
     9481 tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta
     9541 caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt
     9601 gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt
     9661 gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg
     9721 ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca
     9781 tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt
     9841 tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt
     9901 ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat
     9961 tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga
    10021 tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc
    10081 gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa
    10141 taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt
    10201 cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc
    10261 ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg
    10321 tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt
    10381 atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa
    10441 gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc atatcctgct
    10501 gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg
    10561 tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga
    10621 acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt
    10681 gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc
    10741 ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg
    10801 acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact
    10861 tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc
    10921 acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg
    10981 gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg
    11041 gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac
    11101 atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga
    11161 ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga
    11221 atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa
    11281 aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt
    11341 ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat
    11401 ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac
    11461 gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag
    11521 ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg
    11581 ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg
    11641 caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc
    11701 caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg
    11761 cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca
    11821 cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt
    11881 cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca
    11941 gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt
    12001 cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta
    12061 catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa
    12121 tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg
    12181 tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc
    12241 cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca
    12301 taattactat cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt
    12361 ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc
    12421 tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct
    12481 gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata
    12541 taaaatagcc actatagatg aatttttcta cgtggctgta agtcgtgttt aaaaatcttt
    12601 agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat
    12661 ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat
    12721 cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc
    12781 atttttatga aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca
    12841 attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct
    12901 tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa
    12961 tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg
    13021 taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca
    13081 aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc
    13141 agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat
    13201 cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt
    13261 tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg
    13321 gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt
    13381 tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa
    13441 cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt
    13501 ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt
    13561 tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt
    13621 actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact
    13681 tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg
    13741 tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt
    13801 ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc
    13861 cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata
    13921 tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc
    13981 ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc
    14041 aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc
    14101 ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt
    14161 catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat
    14221 gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat
    14281 gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata
    14341 atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc
    14401 ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt
    14461 ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg
    14521 tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt
    14581 cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt
    14641 ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt
    14701 ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac
    14761 cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt
    14821 acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc
    14881 caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc
    14941 atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc
    15001 atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa
    15061 atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc
    15121 ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg
    15181 gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag
    15241 aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc
    15301 ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa
    15361 atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga
    15421 accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt
    15481 cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg
    15541 gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca
    15601 ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca
    15661 aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct
    15721 tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg
    15781 atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca taactttttg
    15841 aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc
    15901 atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca
    15961 tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct
    16021 aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct
    16081 tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga
    16141 taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc
    16201 agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat
    16261 ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa
    16321 gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc
    16381 catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag
    16441 ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg
    16501 agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca
    16561 tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc
    16621 tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt
    16681 tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt
    16741 cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat
    16801 cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac
    16861 tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct
    16921 aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca
    16981 ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat
    17041 tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg
    17101 ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca
    17161 ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc
    17221 tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct
    17281 tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga
    17341 ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa
    17401 atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta
    17461 tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca
    17521 tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa
    17581 tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac
    17641 ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa
    17701 tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg
    17761 ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac
    17821 agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa
    17881 tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg
    17941 ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc
    18001 ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca
    18061 ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa
    18121 caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta
    18181 gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg
    18241 atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt
    18301 tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga
    18361 tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag
    18421 ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg
    18481 ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg
    18541 ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata
    18601 accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa
    18661 ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata
    18721 aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg
    18781 cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg
    18841 gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc
    18901 ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga
    18961 aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga
    19021 caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc
    19081 aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt
    19141 tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt
    19201 gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc
    19261 acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat
    19321 tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa
    19381 aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc
    19441 tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca
    19501 tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc
    19561 aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt
    19621 gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt
    19681 ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca
    19741 tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct
    19801 agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga
    19861 acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg
    19921 ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg
    19981 aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag
    20041 tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc
    20101 acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac
    20161 atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca
    20221 aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc
    20281 ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta
    20341 tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca
    20401 aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc
    20461 cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc
    20521 ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag
    20581 acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta
    20641 tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc
    20701 gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt
    20761 agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa
    20821 ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc
    20881 acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaac
//
LOCUS       DAYYHB010000044        19996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000044 DAYYHB010000000
VERSION     DAYYHB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.045"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACHA6B_001600"
     CDS             4..816
                     /locus_tag="ACHA6B_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8949174.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACHA6B_001601"
     CDS             813..1613
                     /locus_tag="ACHA6B_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8949175.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACHA6B_001602"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACHA6B_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8949176.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACHA6B_001603"
     CDS             3110..4819
                     /locus_tag="ACHA6B_001603"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8949177.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ACHA6B_001604"
     CDS             5031..9383
                     /locus_tag="ACHA6B_001604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8949178.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ACHA6B_001605"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ACHA6B_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8949179.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ACHA6B_001606"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ACHA6B_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8949180.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ACHA6B_001607"
     CDS             11221..11514
                     /locus_tag="ACHA6B_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8949181.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ACHA6B_001608"
     CDS             11511..11822
                     /locus_tag="ACHA6B_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8949182.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ACHA6B_001609"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ACHA6B_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8949183.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ACHA6B_001610"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ACHA6B_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8949184.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="ACHA6B_001611"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="ACHA6B_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="ACHA6B_001612"
     CDS             15292..17655
                     /locus_tag="ACHA6B_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8949185.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="ACHA6B_001613"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="ACHA6B_001613"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8949186.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="ACHA6B_001614"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="ACHA6B_001614"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8949187.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3513 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DAYYHB010000045        19988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000045 DAYYHB010000000
VERSION     DAYYHB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.046"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..506
                     /locus_tag="ACHA6B_001615"
     CDS             72..506
                     /locus_tag="ACHA6B_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949188.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="ACHA6B_001616"
     CDS             complement(584..1117)
                     /locus_tag="ACHA6B_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8949189.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="ACHA6B_001617"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="ACHA6B_001617"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8949190.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="ACHA6B_001618"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="ACHA6B_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8949191.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="ACHA6B_001619"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="ACHA6B_001619"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8949192.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="ACHA6B_001620"
     CDS             complement(4842..5420)
                     /locus_tag="ACHA6B_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8949193.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="ACHA6B_001621"
     CDS             5742..6212
                     /locus_tag="ACHA6B_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8949194.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="ACHA6B_001622"
     CDS             complement(6320..7546)
                     /locus_tag="ACHA6B_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8949195.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="ACHA6B_001623"
     CDS             complement(8300..8500)
                     /locus_tag="ACHA6B_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8949196.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="ACHA6B_001624"
     CDS             complement(8634..9242)
                     /locus_tag="ACHA6B_001624"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8949197.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="ACHA6B_001625"
     CDS             complement(9327..9797)
                     /locus_tag="ACHA6B_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8949198.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="ACHA6B_001626"
     CDS             complement(9845..10759)
                     /locus_tag="ACHA6B_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8949199.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="ACHA6B_001627"
     CDS             10905..11708
                     /locus_tag="ACHA6B_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8949200.1"
                     /translation="MTTKQLIISSFIRFKDLKESRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="ACHA6B_001628"
     CDS             11886..12833
                     /locus_tag="ACHA6B_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8949201.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="ACHA6B_001629"
     CDS             complement(12901..13194)
                     /locus_tag="ACHA6B_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8949202.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="ACHA6B_001630"
     CDS             complement(13222..13887)
                     /locus_tag="ACHA6B_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8949203.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="ACHA6B_001631"
     CDS             complement(14027..14659)
                     /locus_tag="ACHA6B_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8949204.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /gene="liaS"
                     /locus_tag="ACHA6B_001632"
     CDS             complement(14666..15733)
                     /gene="liaS"
                     /locus_tag="ACHA6B_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8949205.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /gene="liaF"
                     /locus_tag="ACHA6B_001633"
     CDS             complement(15730..16461)
                     /gene="liaF"
                     /locus_tag="ACHA6B_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8949206.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="ACHA6B_001634"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="ACHA6B_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8949207.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="ACHA6B_001635"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="ACHA6B_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8949208.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="ACHA6B_001636"
     CDS             18624..19793
                     /locus_tag="ACHA6B_001636"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8949209.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         6041 a         4005 c         3215 g         6727 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga gtcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttacc
//
LOCUS       DAYYHB010000046        18982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000046 DAYYHB010000000
VERSION     DAYYHB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.047"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..309)
                     /locus_tag="ACHA6B_001637"
     CDS             complement(145..309)
                     /locus_tag="ACHA6B_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949210.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            604..1932
                     /locus_tag="ACHA6B_001638"
     CDS             604..1932
                     /locus_tag="ACHA6B_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8949211.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2134..2967
                     /locus_tag="ACHA6B_001639"
     CDS             2134..2967
                     /locus_tag="ACHA6B_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8949212.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2983..3720
                     /locus_tag="ACHA6B_001640"
     CDS             2983..3720
                     /locus_tag="ACHA6B_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8949213.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3875..4843)
                     /locus_tag="ACHA6B_001641"
     CDS             complement(3875..4843)
                     /locus_tag="ACHA6B_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8949214.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5075..5908
                     /locus_tag="ACHA6B_001642"
     CDS             5075..5908
                     /locus_tag="ACHA6B_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8949215.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5942..6781
                     /locus_tag="ACHA6B_001643"
     CDS             5942..6781
                     /locus_tag="ACHA6B_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8949216.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6807..7367
                     /locus_tag="ACHA6B_001644"
     CDS             6807..7367
                     /locus_tag="ACHA6B_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8949217.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7566..9287
                     /locus_tag="ACHA6B_001645"
     CDS             7566..9287
                     /locus_tag="ACHA6B_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8949218.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9452..10594
                     /locus_tag="ACHA6B_001646"
     CDS             9452..10594
                     /locus_tag="ACHA6B_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8949219.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10610..11821
                     /gene="thiI"
                     /locus_tag="ACHA6B_001647"
     CDS             10610..11821
                     /gene="thiI"
                     /locus_tag="ACHA6B_001647"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8949220.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12394..13041
                     /locus_tag="ACHA6B_001648"
     CDS             12394..13041
                     /locus_tag="ACHA6B_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8949221.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13170..13386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13434..16079
                     /locus_tag="ACHA6B_001649"
     CDS             13434..16079
                     /locus_tag="ACHA6B_001649"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8949222.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16389..17702
                     /locus_tag="ACHA6B_001650"
     CDS             16389..17702
                     /locus_tag="ACHA6B_001650"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8949223.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17692..18345
                     /locus_tag="ACHA6B_001651"
     CDS             17692..18345
                     /locus_tag="ACHA6B_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8949224.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18400..18951
                     /gene="radC"
                     /locus_tag="ACHA6B_001652"
                     /pseudo
     CDS             18400..18951
                     /gene="radC"
                     /locus_tag="ACHA6B_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         6391 a         3206 c         4012 g         5373 t
ORIGIN      
        1 gccggagtaa ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc
       61 ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga
      121 aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata
      181 aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc
      241 aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag
      301 gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat
      361 tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat
      421 tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat
      481 ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga
      541 ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat
      601 tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat
      661 atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa
      721 aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa
      781 gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt
      841 cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt
      901 ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa
      961 gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc
     1021 caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg
     1081 ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg
     1141 cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt
     1201 gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga
     1261 acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac
     1321 cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat
     1381 gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag
     1441 atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact
     1501 ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat
     1561 gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat
     1621 gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt
     1681 ctgatgaccc atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa
     1741 tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt
     1801 gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa
     1861 gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt
     1921 gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct
     1981 taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata
     2041 attctaaata aaggataagg attggaaata atccttttat cattaaaaat atgatgacga
     2101 agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat
     2161 ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt
     2221 ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt
     2281 tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg
     2341 tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga
     2401 aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca
     2461 caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa
     2521 gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa
     2581 gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta
     2641 ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt
     2701 ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat
     2761 gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt
     2821 ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt
     2881 taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa
     2941 gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc
     3001 aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg
     3061 atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt
     3121 gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat ggagattaat
     3181 cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag
     3241 tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata
     3301 gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag
     3361 atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa
     3421 aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat
     3481 cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt
     3541 ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg
     3601 aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat
     3661 gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa
     3721 aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg
     3781 tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc
     3841 tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt
     3901 cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat
     3961 taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat
     4021 cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt
     4081 ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag
     4141 cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca
     4201 tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct
     4261 ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac
     4321 ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg
     4381 cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac
     4441 ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg
     4501 aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa
     4561 aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg
     4621 cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg
     4681 agggaagatt gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg
     4741 catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca
     4801 tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac
     4861 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     4921 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     4981 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     5041 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     5101 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     5161 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     5221 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     5281 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     5341 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     5401 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     5461 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     5521 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     5581 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     5641 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     5701 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     5761 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     5821 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     5881 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     5941 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     6001 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     6061 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     6121 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     6181 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     6241 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     6301 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     6361 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     6421 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     6481 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     6541 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     6601 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     6661 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     6721 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     6781 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     6841 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     6901 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     6961 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     7021 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     7081 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     7141 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     7201 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     7261 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     7321 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     7381 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     7441 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     7501 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     7561 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     7621 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     7681 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     7741 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     7801 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     7861 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     7921 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     7981 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     8041 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     8101 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     8161 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     8221 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     8281 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     8341 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     8401 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     8461 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     8521 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     8581 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     8641 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     8701 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     8761 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     8821 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     8881 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     8941 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     9001 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     9061 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     9121 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     9181 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     9241 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     9301 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     9361 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     9421 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     9481 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     9541 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     9601 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     9661 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     9721 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     9781 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     9841 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     9901 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     9961 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
    10021 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
    10081 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
    10141 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
    10201 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
    10261 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
    10321 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
    10381 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
    10441 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
    10501 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
    10561 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
    10621 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
    10681 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
    10741 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
    10801 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
    10861 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
    10921 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
    10981 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
    11041 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
    11101 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
    11161 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
    11221 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
    11281 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
    11341 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
    11401 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
    11461 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
    11521 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
    11581 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
    11641 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
    11701 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
    11761 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
    11821 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
    11881 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
    11941 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
    12001 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
    12061 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
    12121 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
    12181 ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    12241 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
    12301 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
    12361 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
    12421 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca
    12481 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
    12541 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
    12601 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
    12661 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
    12721 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
    12781 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
    12841 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
    12901 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
    12961 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
    13021 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
    13081 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
    13141 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
    13201 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
    13261 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
    13321 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
    13381 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
    13441 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
    13501 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
    13561 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
    13621 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
    13681 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
    13741 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
    13801 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
    13861 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
    13921 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
    13981 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
    14041 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
    14101 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
    14161 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
    14221 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
    14281 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
    14341 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
    14401 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
    14461 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
    14521 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
    14581 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
    14641 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
    14701 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
    14761 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
    14821 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
    14881 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
    14941 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
    15001 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
    15061 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
    15121 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
    15181 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
    15241 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
    15301 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
    15361 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
    15421 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
    15481 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
    15541 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
    15601 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
    15661 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
    15721 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
    15781 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
    15841 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
    15901 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
    15961 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
    16021 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
    16081 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
    16141 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
    16201 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
    16261 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
    16321 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
    16381 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
    16441 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
    16501 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
    16561 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
    16621 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
    16681 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
    16741 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
    16801 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
    16861 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
    16921 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
    16981 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
    17041 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
    17101 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
    17161 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
    17221 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
    17281 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
    17341 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
    17401 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
    17461 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
    17521 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
    17581 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
    17641 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
    17701 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
    17761 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
    17821 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
    17881 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
    17941 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
    18001 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
    18061 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
    18121 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
    18181 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
    18241 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
    18301 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
    18361 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
    18421 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
    18481 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
    18541 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
    18601 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
    18661 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
    18721 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
    18781 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
    18841 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
    18901 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgtttt aaccatagta
    18961 taataaataa tagtagttaa ag
//
LOCUS       DAYYHB010000047        18221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000047 DAYYHB010000000
VERSION     DAYYHB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.048"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACHA6B_001653"
     CDS             <1..205
                     /locus_tag="ACHA6B_001653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949225.1"
                     /translation="PPDFLIAQVYLEVHLLVRYCSMGRLLNTEKMVFEHHLFKIKKDC
                     DTNFVTTFKCIPRAKKLSEEIQF"
     gene            complement(239..544)
                     /locus_tag="ACHA6B_001654"
     CDS             complement(239..544)
                     /locus_tag="ACHA6B_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949226.1"
                     /translation="MTKEVEIMKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACAD
                     YNENELFILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(541..1431)
                     /locus_tag="ACHA6B_001655"
     CDS             complement(541..1431)
                     /locus_tag="ACHA6B_001655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8949227.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1451..1669)
                     /locus_tag="ACHA6B_001656"
     CDS             complement(1451..1669)
                     /locus_tag="ACHA6B_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8949228.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1639..3339)
                     /locus_tag="ACHA6B_001657"
     CDS             complement(1639..3339)
                     /locus_tag="ACHA6B_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8949229.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3606..4379
                     /locus_tag="ACHA6B_001658"
     CDS             3606..4379
                     /locus_tag="ACHA6B_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949230.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="ACHA6B_001659"
     CDS             complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="ACHA6B_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8949231.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5095..5197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5203..6174)
                     /locus_tag="ACHA6B_001660"
     CDS             complement(5203..6174)
                     /locus_tag="ACHA6B_001660"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8949232.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="ACHA6B_001661"
     CDS             complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="ACHA6B_001661"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8949233.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8144..8962)
                     /gene="purR"
                     /locus_tag="ACHA6B_001662"
     CDS             complement(8144..8962)
                     /gene="purR"
                     /locus_tag="ACHA6B_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8949234.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9129..9953)
                     /locus_tag="ACHA6B_001663"
     CDS             complement(9129..9953)
                     /locus_tag="ACHA6B_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8949235.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9926..10606)
                     /locus_tag="ACHA6B_001664"
     CDS             complement(9926..10606)
                     /locus_tag="ACHA6B_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8949236.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10629..11567)
                     /locus_tag="ACHA6B_001665"
     CDS             complement(10629..11567)
                     /locus_tag="ACHA6B_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8949237.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11812..12663)
                     /gene="ispE"
                     /locus_tag="ACHA6B_001666"
     CDS             complement(11812..12663)
                     /gene="ispE"
                     /locus_tag="ACHA6B_001666"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8949238.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="ACHA6B_001667"
     CDS             complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="ACHA6B_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8949239.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15531..16337
                     /gene="proC"
                     /locus_tag="ACHA6B_001668"
     CDS             15531..16337
                     /gene="proC"
                     /locus_tag="ACHA6B_001668"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8949240.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16416..17972)
                     /gene="rny"
                     /locus_tag="ACHA6B_001669"
     CDS             complement(16416..17972)
                     /gene="rny"
                     /locus_tag="ACHA6B_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8949241.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         5252 a         3923 c         3129 g         5917 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactta ataccgagaa aatggttttt gaacatcact tattcaaaat
      121 aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa
      181 gctaagcgaa gaaatccaat tctgattatt tattgtattt ctttgttcag catccttgct
      241 aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg
      301 ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca
      361 tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac
      421 aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag
      481 tttttccact taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg
      541 tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac
      601 ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat
      661 ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc
      721 tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa
      781 caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc
      841 cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag
      901 tttttttagt acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg
      961 accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc
     1021 taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc
     1081 gtccagcgcg aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg
     1141 ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac
     1201 gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg
     1261 gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg
     1321 acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg
     1381 atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg
     1441 tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca
     1501 atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg
     1561 gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg
     1621 acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc
     1681 cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg
     1741 ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg
     1801 ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg
     1861 accaatttgt gccaataaat catccggcat actttttata tcttgttggc agatcatttg
     1921 aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag
     1981 ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag
     2041 tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa
     2101 gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca
     2161 gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa
     2221 ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt
     2281 ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc
     2341 tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg
     2401 atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa
     2461 tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt
     2521 cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt
     2581 atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga
     2641 aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc
     2701 acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt
     2761 tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc
     2821 acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca
     2881 cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata
     2941 gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa
     3001 ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg
     3061 agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa
     3121 cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt
     3181 tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact
     3241 ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag
     3301 cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa
     3361 gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa
     3421 gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg
     3481 aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa
     3541 caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga
     3601 acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa
     3661 aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct
     3721 tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc
     3781 gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg
     3841 aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa
     3901 tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata
     3961 aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc
     4021 gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata
     4081 agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg
     4141 aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag
     4201 ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat tatatttgga
     4261 aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc tactctgtca
     4321 ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca ttgaagtaga
     4381 tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag
     4441 tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa atcgaaggag
     4501 tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc aacccgctgg
     4561 caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca tacgctcccc
     4621 agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca atcaatgcac
     4681 caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct ttttggacat
     4741 ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca
     4801 cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg ccccaaatga
     4861 aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc aatggaatct
     4921 gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac aacaccatcg
     4981 ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata aaaaaacttc
     5041 ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt caaacagctg
     5101 tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt agaaatagat
     5161 tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat tttttgtttc
     5221 aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc ccattagtcc
     5281 gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat agatcgagtc
     5341 tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag ggccggataa
     5401 tactggatgt gtacatgaag catacacact agttgcacca gcttctttca aagcattggc
     5461 agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac aagttttgcc
     5521 tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac ggcgtttgtc
     5581 tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc
     5641 atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta agaaataatt
     5701 ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac cttggatttg
     5761 aactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa tcatgtttgc
     5821 tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc
     5881 ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat
     5941 gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa taacatagac
     6001 atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac taaattgagt
     6061 aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg ctaatgggcg
     6121 attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg acatggggac
     6181 ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc atatttttta
     6241 aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt ttgcatatcc
     6301 ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa tattgtctgt
     6361 aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc cgatgatatt
     6421 cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc
     6481 atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg tagcatcacc
     6541 aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt tcacttccac
     6601 aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg
     6661 tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt caatgacgac
     6721 gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac aatcagaacc
     6781 aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc
     6841 tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat tgaccatatg
     6901 catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga tccgatcatt
     6961 gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat ggccttcttc
     7021 ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt ttgttccaac
     7081 tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg tattgatttc
     7141 ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt ctacgatccc
     7201 aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat
     7261 cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt
     7321 caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt ccaagaaaga
     7381 agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg catatttact
     7441 gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg taatcacttc
     7501 atctggtttc gtttcactga cccgattaat gatatgttct accataggtt taccagaaac
     7561 gggatgtaaa actttatata attttgactt catgcgggta cctttccctg ccgccaaaat
     7621 aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga tttcagttta
     7681 tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca gcgttttctg
     7741 gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg ctctcctgcc
     7801 aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt ttgaaatcgt
     7861 ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg tttttcaatc
     7921 agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt tttccgttta
     7981 ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac ggtgttcaaa
     8041 agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa
     8101 atttttctta ctactcgaaa ctaagcagct ttttcacaac ctcttattct gaaaaataat
     8161 ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca taaagcagtg
     8221 acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat acagtgatgc
     8281 caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact gttccgccgc
     8341 ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt ctcttagaca
     8401 gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt actgttgatc
     8461 cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga taataggaca
     8521 cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg attttttcac
     8581 caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt tctcctaaca
     8641 aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat agacgttctg
     8701 ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg aacaatacac
     8761 cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta aatgttttct
     8821 tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt tcggcaaagc
     8881 ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg tcaactagac
     8941 gttcactgcg acgtactttc acctattcca actcctttta agaaatttta tcgttttaca
     9001 ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa aatagttcgg
     9061 tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag gcttcttttc
     9121 tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat gatatttaca
     9181 atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt atctagataa
     9241 aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact gatagcaatc
     9301 acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac catgattgcg
     9361 gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc agtaatcaca
     9421 ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt atcttcatca
     9481 aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat ggcaacgaat
     9541 aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc aaacagatag
     9601 gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa cgctagcgcc
     9661 aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta gatcgttcgc
     9721 agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag tgtaggatta
     9781 aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag cgtatcagcc
     9841 atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat tccggcaatg
     9901 aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc cattgagaat
     9961 cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct tcatgatcat
    10021 gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga agacgataaa
    10081 attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca aataagtcag
    10141 ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca gacaattcac
    10201 ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc tgtacatgtt
    10261 gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt cctgaacgga
    10321 ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat
    10381 aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc attccttttg
    10441 aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct ccattttctc
    10501 cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct agtacaggtt
    10561 catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta caacctccat
    10621 tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat gacagaaata
    10681 taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt caacacttct
    10741 agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc attttcttca
    10801 aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc taagcgactt
    10861 ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga
    10921 ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt tgcatctttt
    10981 agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc atcggcattc
    11041 ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc aatcgttctg
    11101 acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc atgtgaatga
    11161 ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa acggtcgatt
    11221 cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt taacgaatcc
    11281 acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga aatttctgct
    11341 ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat caaaagtttc
    11401 acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata aaaagtcgta
    11461 acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca tgctgcaaac
    11521 agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta tatgctcctt
    11581 tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa
    11641 aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct tgattaaatc
    11701 gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa aactgaagaa
    11761 taccatttta ggattcctca gtttttcatc acctatttta cttcttatat tttattgcag
    11821 ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata ctctctgcgc
    11881 tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca tcaatgcagc
    11941 atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat gccgctttcc
    12001 cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat cttgctctcg
    12061 gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat ctacttctct
    12121 aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc agcactgtgg
    12181 catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg tacttccgta
    12241 gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt cttctaacga
    12301 acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt ctgtacttcc
    12361 tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga caccggtttt
    12421 gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc gttcatctac
    12481 cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt cttcaaaaaa
    12541 tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat acccatcttg
    12601 ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt caatcaattc
    12661 catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa
    12721 agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc tttcacagaa
    12781 ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga gcacttcttc
    12841 tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct gcatggtagc
    12901 aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat gatcgacata
    12961 ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat aattatatac
    13021 gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat tagctaatgg
    13081 agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa acagattggc
    13141 taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca aatcatatcc
    13201 cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt gatctttacg
    13261 gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca cagagaagta
    13321 tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt gatgtaattc
    13381 ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac gttgatattc
    13441 aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc
    13501 tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt ctgatatctt
    13561 ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc
    13621 ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt ttcctttgat
    13681 ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat aactgtaacc
    13741 tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata aacggtcaaa
    13801 acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat attgataaag
    13861 cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc acacatcatc
    13921 tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc gtccgctgtg
    13981 atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat caaagattat
    14041 agctccccaa gaataaacga attttgttcg ataaaattct tttctggata accacacagc
    14101 taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt
    14161 taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga cttttactga
    14221 aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg tctgatctaa
    14281 aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag acagattagt
    14341 gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta cgagcacgat
    14401 atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat tgattttgac
    14461 tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat aataaaacaa
    14521 ccgataatat aatagccaaa ctactgctac atcttgccga acaggcaatt gactcattcc
    14581 taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca gcattcctat
    14641 caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta ttctcatcaa
    14701 cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg atgccagtag
    14761 cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct ttagataagc
    14821 aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc aatagcttcg
    14881 aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg accatacaga
    14941 caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca tttttctatt
    15001 tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac caaaaccggc
    15061 gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc tttccatttt
    15121 tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca acatcggagt
    15181 gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt gttgtcccat
    15241 cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt gatttgccac
    15301 aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt cctttatcca
    15361 ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg tatacttttg
    15421 tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc tgttctttgc
    15481 ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac atgaaagtag
    15541 gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta aaaaaaggaa
    15601 cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca caaaaatttg
    15661 ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca caatcagata
    15721 tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat
    15781 atagccagaa agtcttattc atttctattg cttcaggaaa atcattaaca gatttagaaa
    15841 cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg
    15901 taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa cagcttcagc
    15961 aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa tcagatttca
    16021 gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg atcgatgcga
    16081 tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag attgctgcaa
    16141 aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca tgggaacttg
    16201 tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag
    16261 caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga
    16321 tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg
    16381 tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc agttgcacga
    16441 gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc ttcgattcgt
    16501 ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc tggcttgacc
    16561 attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc ggcgaagctg
    16621 ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga ttcacttcgc
    16681 gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga gataacagaa
    16741 gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg gttttctttg
    16801 tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc atggtcaagt
    16861 gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc ttcgccaagc
    16921 tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa gacgttttgt
    16981 ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg atgaagtgta
    17041 tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc atccatttct
    17101 ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc atcttgtatc
    17161 agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc ggaaagaaca
    17221 actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc aagcgttcga
    17281 atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg aagggtaacg
    17341 actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc taatgataat
    17401 aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc ttttaccatt
    17461 agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc
    17521 tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat cattttttca
    17581 acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc ttctttactt
    17641 tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt ttgttcgcgt
    17701 tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag ctcactttca
    17761 acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc tttttttaga
    17821 gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg tgcaccagct
    17881 atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc gacaattaaa
    17941 ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact atctttttca
    18001 tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat atgcatcttc
    18061 gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc ccgtagtgtc
    18121 aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg tttttaaaat
    18181 aaatgacact agtttcatat aggtaaacct ttaaattgag a
//
LOCUS       DAYYHB010000048        17784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000048 DAYYHB010000000
VERSION     DAYYHB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.049"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..1192
                     /locus_tag="ACHA6B_001670"
     CDS             227..1192
                     /locus_tag="ACHA6B_001670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8949242.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1291..2463)
                     /locus_tag="ACHA6B_001671"
     CDS             complement(1291..2463)
                     /locus_tag="ACHA6B_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949243.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2450..3595)
                     /locus_tag="ACHA6B_001672"
     CDS             complement(2450..3595)
                     /locus_tag="ACHA6B_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949244.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3609..4541)
                     /locus_tag="ACHA6B_001673"
     CDS             complement(3609..4541)
                     /locus_tag="ACHA6B_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949245.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4658..5737
                     /locus_tag="ACHA6B_001674"
     CDS             4658..5737
                     /locus_tag="ACHA6B_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8949246.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5734..6363
                     /locus_tag="ACHA6B_001675"
     CDS             5734..6363
                     /locus_tag="ACHA6B_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8949247.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6473..6700)
                     /locus_tag="ACHA6B_001676"
     CDS             complement(6473..6700)
                     /locus_tag="ACHA6B_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8949248.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6803..9391)
                     /locus_tag="ACHA6B_001677"
     CDS             complement(6803..9391)
                     /locus_tag="ACHA6B_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8949249.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9411..10070)
                     /locus_tag="ACHA6B_001678"
     CDS             complement(9411..10070)
                     /locus_tag="ACHA6B_001678"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8949250.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10119..10622)
                     /gene="trmL"
                     /locus_tag="ACHA6B_001679"
     CDS             complement(10119..10622)
                     /gene="trmL"
                     /locus_tag="ACHA6B_001679"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8949251.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10843..11709)
                     /locus_tag="ACHA6B_001680"
     CDS             complement(10843..11709)
                     /locus_tag="ACHA6B_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8949252.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11818..12459
                     /locus_tag="ACHA6B_001681"
     CDS             11818..12459
                     /locus_tag="ACHA6B_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8949253.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12521..13882)
                     /gene="mgtE"
                     /locus_tag="ACHA6B_001682"
     CDS             complement(12521..13882)
                     /gene="mgtE"
                     /locus_tag="ACHA6B_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8949254.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13900..14799)
                     /locus_tag="ACHA6B_001683"
     CDS             complement(13900..14799)
                     /locus_tag="ACHA6B_001683"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8949255.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14801..15598)
                     /locus_tag="ACHA6B_001684"
     CDS             complement(14801..15598)
                     /locus_tag="ACHA6B_001684"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8949256.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15576..16280)
                     /locus_tag="ACHA6B_001685"
     CDS             complement(15576..16280)
                     /locus_tag="ACHA6B_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8949257.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16411..16986
                     /locus_tag="ACHA6B_001686"
     CDS             16411..16986
                     /locus_tag="ACHA6B_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8949258.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17104..17781
                     /locus_tag="ACHA6B_001687"
     CDS             17104..17781
                     /locus_tag="ACHA6B_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8949259.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5260 a         3813 c         3162 g         5549 t
ORIGIN      
        1 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
       61 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
      121 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
      181 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
      241 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
      301 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
      361 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
      421 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
      481 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
      541 agcaggttct ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
      601 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
      661 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
      721 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
      781 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
      841 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
      901 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
      961 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
     1021 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
     1081 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
     1141 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
     1201 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
     1261 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
     1321 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
     1381 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
     1441 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
     1501 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
     1561 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
     1621 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
     1681 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
     1741 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
     1801 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaacgcta gattgatgac
     1861 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
     1921 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
     1981 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
     2041 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
     2101 atctgggcga ctctgtatct tgatcgtcgc catctttcct acttcgactt gttcggtaaa
     2161 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
     2221 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
     2281 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
     2341 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
     2401 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
     2461 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
     2521 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
     2581 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
     2641 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
     2701 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
     2761 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
     2821 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
     2881 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
     2941 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
     3001 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
     3061 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
     3121 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
     3181 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
     3241 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
     3301 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
     3361 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
     3421 ccgctttgat tacaattcca gtattaatcg tttcaagatt gccaccttga tcaatctcgc
     3481 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
     3541 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
     3601 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
     3661 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
     3721 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
     3781 attccatcag aagctattgt tcgctgaatc gctgacaaca cattttctgg gagtaatggg
     3841 atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct
     3901 ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct
     3961 tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt
     4021 tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt
     4081 aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta
     4141 agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca
     4201 atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt
     4261 tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc
     4321 ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca
     4381 taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggacct
     4441 aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa
     4501 tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa
     4561 atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa
     4621 accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa
     4681 cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc
     4741 tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc
     4801 tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga
     4861 tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga
     4921 ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa
     4981 ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg
     5041 aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca
     5101 agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca
     5161 aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct
     5221 gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa
     5281 agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag
     5341 tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc
     5401 tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca
     5461 gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca
     5521 tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac
     5581 gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg
     5641 tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca
     5701 gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat tattgatgat
     5761 gatccatttg tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg
     5821 ttaggcattg gtcattgtgc caaggatgcg ctagtcttat acgaaaccca tcgtcccgat
     5881 gttttgctga cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt
     5941 ttggctaaat ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac
     6001 atccgcgaag cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg
     6061 attattcctt ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc
     6121 gagactttca ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct
     6181 tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa
     6241 attgcagatg ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta
     6301 gaaaagctag atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa
     6361 taagtcttat ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt
     6421 tcacatcttc tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat
     6481 tcgcagggct gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct
     6541 tgtctaacgt tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt
     6601 cattcaattt gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt
     6661 ttgctaattt cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg
     6721 atttcagctt agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt
     6781 tagctatttc tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac
     6841 tgttttccat accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa
     6901 cgaaatcttt agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag
     6961 aaaacagatc tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt
     7021 catcttctac aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt
     7081 caaatggatc atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga
     7141 tattcatttt atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt
     7201 cattcgctac acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac
     7261 tgcgagtcac tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca
     7321 tgggcaaaat aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag
     7381 aaagagtgat tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga
     7441 taactaattc atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc
     7501 ccatatctcc attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc
     7561 ctatccgata aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga
     7621 atatctcctg catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg
     7681 ctaatacttg gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag
     7741 agatcaaggg ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct
     7801 ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag
     7861 aaccatcacc ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca
     7921 aaagatcatg gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta
     7981 agataacttg catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga
     8041 ccatagacat ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag
     8101 gtgttttttc ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg
     8161 tcgtttcgtt cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa
     8221 tttcttgggt atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga
     8281 cgattccgtt gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag
     8341 gggaacggat tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata
     8401 ttttttcgat acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt
     8461 tacgactgag aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt
     8521 tttcgtaaag attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta
     8581 cagtagcaac ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg
     8641 tttgctgaag caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa
     8701 aaatctgatg caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt
     8761 gttcggctag attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt
     8821 acggattttc ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag
     8881 ctaactgact accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt
     8941 ttagccgaat cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt
     9001 ttaataccga aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa
     9061 ttttttctgc tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga
     9121 tcccatttgc agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt
     9181 atttaggatg atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc
     9241 cgaaacttcc ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt
     9301 gtaccaaaag cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac
     9361 caacgaaata aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa
     9421 ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat
     9481 ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca
     9541 cccatttcac gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa
     9601 gaagctccat gtcctacgaa taaaagtggg ccatctttat gtcgggcaac cgcatctgct
     9661 acaacagttt cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga
     9721 tcatacagat ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt
     9781 tcatcgatcg actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa
     9841 aaaatctcta ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc
     9901 tttgctgtac tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct
     9961 ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt
    10021 ctttcctgat tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa
    10081 ctcctgtctt tcttttattt tactctataa taaactgtct aatccagctt gtcattttca
    10141 tagtgatgcg tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc
    10201 attgcagctg tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt
    10261 aaacactttt cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca
    10321 aataaaaaga agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca
    10381 aattttgaga tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta
    10441 ctgtggtaag taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg
    10501 tcatcagttg aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac
    10561 gtacgggcaa tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc
    10621 atcaggatca ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc
    10681 ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc
    10741 ttctcactaa cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt
    10801 gctaatataa caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa
    10861 acgttttctc ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt
    10921 cttatataag tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa
    10981 aagatctttt tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt
    11041 taatttcatt gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc
    11101 aggttgattc ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac
    11161 cttcaacact tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga
    11221 aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc
    11281 tacacaccaa aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat
    11341 atcaaatcca acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc
    11401 ttctccgcag ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc
    11461 aataacgggc tgatacatgc cagattggat cattctacgc cttggaacga acattccttg
    11521 atcgtattct gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc
    11581 aaaacggtga ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca
    11641 aaccggacag cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt
    11701 ttttaacata gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa
    11761 ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg
    11821 atcaaagaat tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc
    11881 gatcggatcg aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg
    11941 atcgaagaaa cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt
    12001 cctagagggg gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg
    12061 attgaagcaa agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc
    12121 tggctagacg aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc
    12181 ttccatatgg cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg
    12241 gtagaacatg gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta
    12301 gaaaatttcc cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg
    12361 cctggtggtg gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc
    12421 gaagtacacg gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt
    12481 tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa
    12541 ataaagaagc aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa
    12601 agggaccgct tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac
    12661 tacctgcaag attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca
    12721 agataaaatt atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc
    12781 ctgtgacaac gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct
    12841 catcagaaat cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg
    12901 cagttcctgt aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt
    12961 catagtgact gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag
    13021 gcaaacgtcg agacgctgcc tttactggat tttcattaat ttcttccaca ttgaccccgg
    13081 caagtcctga ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg
    13141 tgacgattcc taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat
    13201 ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt
    13261 cgctcatcag atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa
    13321 taacccctac tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta
    13381 catctgcttc gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg
    13441 aaacaaattc agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct
    13501 tgatttcact ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat
    13561 ccagcgtatt aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg
    13621 catagctagg acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca
    13681 catcgaacat atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct
    13741 cttctgtaag agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt
    13801 ttctgaatgc ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct
    13861 ctaattcttg attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac
    13921 aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg
    13981 atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg
    14041 cccgccatac atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat
    14101 ttgatgtgtc cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt
    14161 ctctatccag tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag
    14221 cgaagaataa tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag
    14281 ccttccactg actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc
    14341 taattttgcg tgcgcaaatc catgctttgc aaaaagcatc aagccagatg tatcccgatc
    14401 tagtcgcgtc accacatgga ttacttggtt ttcgtaacct tgttttttat aataagcctt
    14461 tacacgatta gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc
    14521 tggtttattt actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc
    14581 caaaagtacg gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt
    14641 agctagagaa aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa
    14701 ttttattttc gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg
    14761 ctgagaatga tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg
    14821 catctttcac acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt
    14881 ctgctattcg ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg
    14941 ttacaagata atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca
    15001 cgatcggaga acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact
    15061 gaaaggcatt gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg
    15121 aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt
    15181 tgatgtatac atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg
    15241 ctaaaaaatg ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat
    15301 aactaacact ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat
    15361 cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat
    15421 tcagacaatg actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga
    15481 tgaccaattc tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca
    15541 aacgttctgt cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc
    15601 cctgcctcct ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat
    15661 tttcctcttc catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac
    15721 atctcttcat ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct
    15781 tctggaaaaa cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc
    15841 atcgcaagag tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt
    15901 acaggatact cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg
    15961 taatctcgtt cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga
    16021 tggatatctt ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct
    16081 tctaaacgtc ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc
    16141 ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg
    16201 cggatccccc gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat
    16261 gcttcccaat ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct
    16321 accatatatg caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt
    16381 aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa
    16441 tctatgctga caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa
    16501 gatttccgtg tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa
    16561 aattgtggcc tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct
    16621 gctgaaaaag ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt
    16681 atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat
    16741 atcgatcctg cttcattaat ccaaaccgga aagcttacaa caaaacgagc tgaattccct
    16801 attgaagaag gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa
    16861 ctggaactag aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    16921 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    16981 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    17041 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    17101 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    17161 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    17221 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    17281 atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca
    17341 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    17401 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    17461 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    17521 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    17581 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    17641 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    17701 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    17761 ttacacttgc tttctaatta aaaa
//
LOCUS       DAYYHB010000049        17443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000049 DAYYHB010000000
VERSION     DAYYHB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.050"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..448
                     /locus_tag="ACHA6B_001688"
     CDS             131..448
                     /locus_tag="ACHA6B_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8949260.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(539..904)
                     /locus_tag="ACHA6B_001689"
     CDS             complement(539..904)
                     /locus_tag="ACHA6B_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949261.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1111..1593
                     /locus_tag="ACHA6B_001690"
     CDS             1111..1593
                     /locus_tag="ACHA6B_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949262.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1790..2680
                     /locus_tag="ACHA6B_001691"
     CDS             1790..2680
                     /locus_tag="ACHA6B_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8949263.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2967..4241)
                     /locus_tag="ACHA6B_001692"
     CDS             complement(2967..4241)
                     /locus_tag="ACHA6B_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8949264.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4514..6985
                     /locus_tag="ACHA6B_001693"
     CDS             4514..6985
                     /locus_tag="ACHA6B_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8949265.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7088..8824)
                     /locus_tag="ACHA6B_001694"
     CDS             complement(7088..8824)
                     /locus_tag="ACHA6B_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8949266.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8821..9525)
                     /locus_tag="ACHA6B_001695"
     CDS             complement(8821..9525)
                     /locus_tag="ACHA6B_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8949267.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9656..10159
                     /locus_tag="ACHA6B_001696"
     CDS             9656..10159
                     /locus_tag="ACHA6B_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8949268.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10119..10457
                     /locus_tag="ACHA6B_001697"
     CDS             10119..10457
                     /locus_tag="ACHA6B_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8949269.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10478..11896
                     /gene="ffh"
                     /locus_tag="ACHA6B_001698"
     CDS             10478..11896
                     /gene="ffh"
                     /locus_tag="ACHA6B_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8949270.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12008..12586
                     /locus_tag="ACHA6B_001699"
     CDS             12008..12586
                     /locus_tag="ACHA6B_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8949271.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12590..13111
                     /locus_tag="ACHA6B_001700"
     CDS             12590..13111
                     /locus_tag="ACHA6B_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8949272.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13190..13744)
                     /locus_tag="ACHA6B_001701"
     CDS             complement(13190..13744)
                     /locus_tag="ACHA6B_001701"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8949273.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13997..14272
                     /gene="rpsP"
                     /locus_tag="ACHA6B_001702"
     CDS             13997..14272
                     /gene="rpsP"
                     /locus_tag="ACHA6B_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8949274.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14284..14535
                     /locus_tag="ACHA6B_001703"
     CDS             14284..14535
                     /locus_tag="ACHA6B_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8949275.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14660..15451
                     /locus_tag="ACHA6B_001704"
     CDS             14660..15451
                     /locus_tag="ACHA6B_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949276.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15621..16142
                     /gene="rimM"
                     /locus_tag="ACHA6B_001705"
     CDS             15621..16142
                     /gene="rimM"
                     /locus_tag="ACHA6B_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8949277.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16132..16893
                     /gene="trmD"
                     /locus_tag="ACHA6B_001706"
     CDS             16132..16893
                     /gene="trmD"
                     /locus_tag="ACHA6B_001706"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8949278.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17029..17376
                     /gene="rplS"
                     /locus_tag="ACHA6B_001707"
     CDS             17029..17376
                     /gene="rplS"
                     /locus_tag="ACHA6B_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8949279.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         5744 a         3122 c         3561 g         5016 t
ORIGIN      
        1 taactatgca ctgtaacgat aatgctgcta aaatatcttt ttacgaaaaa catcacaagg
       61 aaaaacttaa ctatggtatt atctaaacat aattttttcg gtatttaata tttaattagg
      121 aggtaatatt ttgctttttt atagtaattt tatcttaata gtagcgatat tattacttct
      181 gaatatatgg atttttgata ggagtagaaa ttcaagtata ggttttagaa ctaaacgaag
      241 tttgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag gaattgtttt
      301 aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt ccacctcaaa
      361 ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt ttttagtgtt
      421 tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt gatataagaa
      481 aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt
      541 atggatgccg agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg
      601 gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga
      661 agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat
      721 tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag
      781 ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg
      841 cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag caagttttta
      901 tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg taaattgaat
      961 cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac tctcatgcca
     1021 ttatctctaa aaaaaagttt ttccccctaa tttttttaga tagaaaattg tatagtatga
     1081 tgagagaaaa agtagtttgg gggaacgtta atgagaagaa aagcagtaat cgatcggctt
     1141 tatttatttt tttggttttt tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt
     1201 gtacaaggtg cggagatatt aggcctgatc caaggaagcg gatttgtggt ttctaggaat
     1261 tttccagctt tgattgtttt ctatttagtg ataagtgtag gtctcttatt accaaaatct
     1321 ctgatcaatc actacaattg gcagctgctt tacttttctt gcttattagt atttacatct
     1381 atgttttctt tgctgcctcg cttgattatc ggccctaatg gtttgagcga gagtggattg
     1441 aaggatttag cttattcctc catgtatttc ttgacgatca aaccatggtt ctatttagta
     1501 attgcctcat ttattcttgc aacatttttt tacttctgga cagaaatcag gataaaaagt
     1561 gaacgatctg tatctataaa aaataactat tgatggtgga tcatcagtaa aaaaacatat
     1621 gagctgcgta aaatgcatgt ttctcatatg ctttttttat tattatgtaa agaatgcgaa
     1681 aaacgtttac atgattttta cataacttaa ctatattttt acagcaacca tacgagggat
     1741 ttacagtcga ctgataaact ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt
     1801 attgtttact ctaggtatga tgggaattct tttagccgga tgcggctcag gatcaggaaa
     1861 tacagatggc tctgccacac aaaataattc aagtgactca aatgaacaag taaaaatcgt
     1921 tgcggtaggt tcgacagcat tacagccgtt agtcgatgca gcacaagaaa gctttgtaca
     1981 agaaaatcca aattatcaaa tctctgtaca aggaggagga agcggaactg gtttgagcca
     2041 agtcgaagct ggagcagtta ctatcggaaa ctctgatgta tttgcagaag aaagagacgg
     2101 agttgatgct tcaaaattag tggatcataa agtagcagtg gtcgggatgg caccaatcgt
     2161 caataaagat acagatgtca aagatatcac caaacaggaa ttgattgata tctttacagg
     2221 aaaaatcact aactggaagg aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg
     2281 tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc
     2341 tgtgcagtcc caagaacaag attcatcagg aacagttcgg cagctggtaa gtcaaacacc
     2401 aggagcaatc agctatttag ctttctcata tctcgatgat tccacacaag cactaagtat
     2461 cgacggtgta gaaccaaaag aagagaatgt agcagataac agctggggaa tctggtcata
     2521 tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc caaaaattct tagactacat
     2581 gatgacagaa gaaatccaag aaggacctgt caaagaattg ggctatttac caatcacaat
     2641 gatggaagta gaacgtgatc acgaaggaaa tatcaaataa ctagcagata aacatgccac
     2701 ggtagtcgtt actattaagg aaaaatcaac tatgtttccc cacatataca tttttccttt
     2761 aattgtgaaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
     2821 gattgaacac cgtttattcg aaaaaaagag ctacgacata gaactgtcgt aacttttttt
     2881 ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat aaaacttagt ccgatatgcg
     2941 gcgcgttctg tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag
     3001 atccgtgccc gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg
     3061 atcttgttat tcagcccctc agtgacgccg tttgagtggg acaccttaaa ggcatttgaa
     3121 atacccgatt ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta
     3181 gatgaaattg ttttaagggt ttcttgaaag ccttccgtac ctcgtttaac gaaataatag
     3241 cgtaataatt gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca
     3301 tcaacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt
     3361 ggctccaatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac
     3421 ttcttctgat tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga
     3481 ttgaagctgc gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt
     3541 ggaaagactg ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg
     3601 acctttaaac gggcctttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg
     3661 cgatcttcaa ggatcgtaaa tagctgattg gttgtcccat caacacaaat aaaactcatg
     3721 gaaccagaaa cagaattcat tgatttgaac tcatccatac acaacaccgt cggaaggaag
     3781 ttcatacgtg gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc
     3841 gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga
     3901 atcgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca
     3961 gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt
     4021 aacttttctt tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata
     4081 cgaaccgatc gacagtgttc acaataggaa gaagagtcat ccaattttcc tttaataaac
     4141 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
     4201 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
     4261 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
     4321 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaacc
     4381 cttttttttt gctatatgac aggaatgaaa ggtgtttgca agcttaatgt gaggaacact
     4441 ttccatatct gttctatact ggataggaga agtttggaaa tatccagaaa acgaaatcaa
     4501 tcgaggtagc cacatgaaga ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca
     4561 aagatcatca gataaagtca tccgtctttt tccatcgtat aaagaaggat taacggcaga
     4621 tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa
     4681 aacaaatcag caaatcgtat tggaaaacat atttacgtat ttcaatttga tctttcttat
     4741 cttagccatt ttgctttgtt tagtagaatc ctataagaac ttgacttttt taccagttat
     4801 catagccaat acaggaatcg ccatttttca ggaaattcga tccaaaaaga tcttggacga
     4861 actaaatgtt ttaaatacag caaaagtcaa tgtcatacga gacgggatag aacaaacaat
     4921 agatatagaa gaccttgtaa tagatgacat tgttatttta gagactggat atcaaattcc
     4981 agcagatgca gaggtcgtgg aagggaaact gcaagttaat gaggctttat tgacaggcga
     5041 agcagatgaa ataaccaaag aaataggtga tccattaatg tccggcagtt tcattgtttc
     5101 cggaaaagct tacgcccgat tagacaaagt tggagcagat tcgtatattt cccagctgac
     5161 agtcaaagcc aagacaatgg gagaaggaga acagtcggag atgattgctt ctcttaatca
     5221 attgatcaaa tggattggga tcatcattat tcctatcggt atcctgcttt tttctcaaag
     5281 ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat
     5341 cggtatgata cctgaaggac tgtacttgct gacgacgatt gcattagcat taagtgcgac
     5401 taaactagca aagcaaagag ttcttttaca caatatgaaa agcattgaga cattggcaag
     5461 ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca gaaaataaaa tgtctgttca
     5521 aaaaatgatc gtctctaaaa aacaagaaat aatcagtcag tcagatcaat tggaagcatg
     5581 cattgctgac tatgcaaaag cgatggcagg agacaatgca acaatggaag ctgttaaagc
     5641 atattttaca aaaaccactg atcaatccta tcaccatgtt attccttttt cttctgtcca
     5701 aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt
     5761 tttgcgcgat caattgccgg aatatgcgga agagtttgtt tctttcgctg aaaaaggcta
     5821 tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa gaaccagtat tgacagaagc
     5881 agtcacacca ttaggctata ttctgattgc taatcctatc agaaaagaag caagagccac
     5941 atttgattac ttcaaaaaac agcaagtagc catcaaagtg atttctggag ataatccgct
     6001 caccgtatcc aatgtagctg tccaagcagg gatttcacat gctgatcagt acgttgatgc
     6061 atctaaatta tcagaagaag aatatgaagc agctgtcgaa aaatatacag tttttggacg
     6121 cgtcaagcca gaacaaaaga aaatcttcgt tcaattgata aagcgtaaca atacagtagc
     6181 tatgacaggt gatggcgtga atgatattct agccatgaaa gaagcagact gcagtatagc
     6241 gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac ttgattctga
     6301 cttttcacgc atgccagaag ttgttgcaga aggacggcgc gttgtgaaca atattgagcg
     6361 atcagccagc ttgtttttag tgaagaatat tttctccttt ttgctctcac tgttttcagt
     6421 gatctttgct ctgacctatc ctttagaacc atcacaaatc acattgatta gtttgtttac
     6481 tatcggactt ccatctttct tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa
     6541 attcatcatg aatgtgatgg aaaaagctgt gccaggtgga ctaacagata tgatcgtagt
     6601 cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat aaaacagatg tttctacagc
     6661 atctaccatg cttttgattg cagtagggtt cctcgttctc tataaaatct gttcaccgct
     6721 gaataaattc agaagtcaga tcattctttt ctgtgctagc ggtaactttt tttcggtcat
     6781 ctttttgcat aagctgtttt caatcaccgg tatctcagct gtatcacttt tgctgttgag
     6841 cattctattt ttctcggcag attcgatttt taggcatttg accacattgg tagaatattt
     6901 gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagcta aaaaatttct
     6961 atcgatattt aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa
     7021 ccactcgtgt atctttcgaa gcaggatgag tcatccaact ttaaaacgca cgagcagttg
     7081 ctctttttta cattttcgga atggtaacgg tgaaagtcga acctgttcca agatgactgt
     7141 ctacagtaat cgtaccatta aggagttgca cgtagtcttt tacaatcgct aaccctaatc
     7201 cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc
     7261 gttcctgatc ctcaatgtcg atacctatac caaagtcttg gacagaaaac gatagatcat
     7321 ctgttgcctg atagcggata ataatctcgc tgtcgctttt tgaatattgg acagcatttt
     7381 caatcagatt tttgataatt ggataaagga tatctgtttt agtagtaaat ggttcgttct
     7441 ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat tggctgataa ttttgaacga
     7501 tttgctggaa ataaggttct gtataaattg tctggatctc gtaagaattc tcgccatctt
     7561 ttgacagctg gatgatttcc cgaatcagtt tatctaaccg gatcgcatct ttttgcatga
     7621 tctcaagaaa agaagtcaga gtttgcgggt catcttttgc accatccagt aaagtctcgg
     7681 taaaaccaat cagtgaagtc acaggagttt ttaattcgtg agagacgttg ccaacgaaat
     7741 ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc atacgccacc cctagaatct
     7801 gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt
     7861 ctgtaagggt gatctcctga tggatcagcg gatgctcagg agtcacttga tgaatcagct
     7921 gaatgagttt tggctcttga atgacttctg tgtaccgctg ttcaggttga tagcttacca
     7981 cgtttaaatg tagctgcatc tttggattaa gcagtagtaa acgagaatca gcatcatcaa
     8041 tcaggaatac gccaatcatc agttcgttca gtaacgtata taattgctct tctgtggtag
     8101 tacacgcccg atacatctta ctcatttgtt cgctcaacgc attaatcgtc tgatacaatt
     8161 cttcccattg atcgggtgtt tgcatgatta cttccgcctg attaggattt tgcaccattt
     8221 tttttagtac gggcagcacg gttttcaatg gctggttttt ttgatagatc agataataga
     8281 tcattcctgc aagaacaagg aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac
     8341 tctcagttct cggtaaaaaa acgcttgtcg gttctgcgat acgtaaaatg gcttctaact
     8401 ggccactttt tttgactggt aatgcgacat ataaaagatc ctcattcaaa gtagtacttt
     8461 tgcgcaaagc gctacctagg ttcccaccat tcaatacggc tttgatttcg ggacgggagt
     8521 ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa gatctttcca gtagcatcca
     8581 ttaaagtaat ccgttcgttc gactgatgga cgaattcttc caaagcagtt ttgttttgcg
     8641 ctgcttggag gttttcgaca tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt
     8701 agctttcttg ctgttccacg atctgctgtt gaaaataatg ggaaatcaat tgccagccac
     8761 caaaaaacaa actaactaaa agaataagaa agaagagacg ttcataaatt tttttccatt
     8821 tcatcgtttg ggttcctgaa acttatagcc aaatcctcta atagtcacca agtactttgg
     8881 gtgttttgga tctcgctcga ttttttctcg caaatgacta acgtgtacat ctacgattcg
     8941 gctttgtcct gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc ggtcgatcac
     9001 acggtctttt cgtttcatga aataaacaag taattcaaat tctttcggtg ttagttcaat
     9061 cagatggtcg tctacggtca cttgataatt ggtcaaatca gcagtgattt gtccaatcga
     9121 tagatattca ggctcagctt catcttttga ttgcttacga ggttcaattc gtctgaatat
     9181 tgctttcata cgggctaata cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc
     9241 acctatttct aggccaatga tccgatccac ttggtcatct ttagccgtca gcatcaaaat
     9301 cggtgtttca tttttttctt gacgcagccg ctgagtgatc tccatgccat ccatcgttgg
     9361 aagcatcaca tctaaaatga tgaaatcaaa tgattgttct aatgctaatt ctaatccttc
     9421 gcctccatct gtggcactgg ttaccttgta gccttctttt tccaaattga acgtcaagag
     9481 cgttacaata gaaggttcat catcaacaac tagtattttt ttcattcaaa tctcctttaa
     9541 ataggtttca ttctgtttat attttagcat agaatcagta caaagggcta ttttacaaaa
     9601 gaaataaatg gtataatagg aaggcacttt gctgaataga aaggaagagc agtcaatgat
     9661 tggaatcagt gcatgtttag gcggtctggc ctgtcgctat gatggacaga aaaaagaagt
     9721 accagaattg atggaactag ttgaaactgg agaagctgtc atggtctgtc cagaagtgat
     9781 cggcggatta ccaattccta gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt
     9841 gtggaaagat caagcaaaag tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa
     9901 aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat
     9961 gaaggaaaaa agcccttcat gcggaaccca ttcgatttat gacggaagtt tttccggaat
    10021 aaaaaaaaat ggtgccggag tggctactgc ttattttatt cagcaaaaaa tgactgtgct
    10081 ctctgatgaa gaatggctga aaactagagg tgagcagaat ggaaatcgaa aagaccaatc
    10141 gaatgaacgc gctctttgaa ttttattcga ccttattgac agaaaaacaa atgaactaca
    10201 tggaattata ttatgcagat gatttttcat taggtgagat cgcggaagaa tacgaagtaa
    10261 gccgacaagc agtatatgac aatatcaagc ggacaagcaa gatcttggag gattatgaaa
    10321 aaaaactcca tcttttttct aattatattg ttcgagaaca ggtattggaa aatatgactg
    10381 catatattac agataagtac ccggaagata aaaaactact tgaatatgta cagcaaattc
    10441 aagcaatcga agaataaata aaaggaatga agaaaatatg gcatttgaaa gcttaactga
    10501 acgtctccag caggcgatgg gaaaaataag aaaaaaagga aaagtatcag aagctgacgt
    10561 aaaagaaatg atgcgggaaa tccgccttgc cttactagaa gccgatgtta acttgcaagt
    10621 agtcaaagat tttactaagc gcgtacgcga acgtgcggta ggggcagaag tgctggaaag
    10681 tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg
    10741 ttctgaaaca gcagagttaa ataaatcacc aaaaatacca actgttatca tgatggctgg
    10801 tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta gccaattatt taaagaaaaa
    10861 tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat cgaccagctg cgatcgacca
    10921 attgaaagta ttaggtcaac agctagacgt tcctgtattt gatatgggaa ctgaagtcag
    10981 cccggtagag atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa acgactatgt
    11041 attgatcgat acggcaggac gtctgcatat cgatgaaaca ttgatggatg agttgaaaca
    11101 aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca tgactggtca
    11161 agatgccgtc aacgtagctg atagcttcaa ccagcaatta ggtattacag gggtcgtcat
    11221 cactaaactt gatggggata ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg
    11281 cgcaccaatc aaattcatcg gttctggtga aaaattgact gacttagaag tattccaccc
    11341 agatcgtatg gctagccgta tccttggtat gggggatatg ctgacattga ttgaaaaagc
    11401 gcagcaagac tacgacgaga aaaaagcgga agaacttgca cagaaaatgc gggaaaattc
    11461 attcgatttc aatgacttta ttgaacagct tgatcaagtg atgggtatgg gcccactgga
    11521 agatctaatc aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt
    11581 agatccaaaa gatgttgaac gcaaaaaagc gatggtttat tctatgacgc cagcagaaag
    11641 agcaaatcct gatctgttga acccaagccg tcgtcgccga attgctgccg gttctggaaa
    11701 cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa aaatgatgca
    11761 gcaaatgtcc aaaggggata tgaacatccc aggtatggac caaatgtttg gttcaggcgt
    11821 caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa
    11881 gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca gactgaaaat gtctactgcc
    11941 ggagcttttc tatcagcaat ttttattctt tactaataca attactctaa acgaagaggt
    12001 ggcacagatg agatgtccgt ggagtgaaca aacagaaagt atgcggcaat atcatgatac
    12061 tgtctggggt gttcctgaat atgatgacca aaagctgttc cgaaaattga tgcttgatat
    12121 taatcaagcc ggattgagct ggcagacgat tttaaataaa agtgaagcat tcgatgaagc
    12181 atatgatcga ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact
    12241 aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc
    12301 caaagcagtc ttgaaaattc aagaagaaat cggcagtttt tcagagtatc tttggtcgtt
    12361 ttctaatgga aaaatcgttg attcttctta tcattcacaa gaagaagtcc caacaacaag
    12421 tcctctttca gatgaattag caaaagatct aaagaaaaga ggatttaaat ttatcggaag
    12481 cacaacaatc tatgcttttt tagaagctgt cgggatcatc aatgatcacc ttgacagttg
    12541 tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat
    12601 catattttat ggtgctataa gtttagatgg ctatttggca acgaaaacgg atgatcttca
    12661 atggcttttt gacactccaa caggtaagca aactacttat gaagcttttt atgaaacaat
    12721 cgatacgacc atcatgggca gaaaaactta tcaggaagca aaaaaatatt tggagaccga
    12781 agcaatttat ccagagaaaa agaatttcgt tttttccacc cacgcacaat tacaattgcc
    12841 agatgctgag gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac aaatgacaga
    12901 aggaaaagat atctgggttg tgggtggcgg tcgattgatc aaaccgctat tggaacatca
    12961 aatgattgat gaatggtata tccagattac ccctgtatta ttaggggacg ggattccttt
    13021 attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta gacacgacac gattcggtga
    13081 atttatcgaa ttgcattatg tacgaaagta gaagtaaata aaataaaaaa ggcaactata
    13141 tgcagaaatg tcctgtacat agttgccttt tttattaccg ttatgctttt tactctgtta
    13201 atttgttaga aaaatctaga tacgcatcta aaatcgtttg gaagaaactt aatgttcctt
    13261 caacgatatt accattttca tcaattagat tcgcaacgtt tccgatatat gcttccggtt
    13321 gctgcaaggt tggcacattc aaaaagacaa gtgattggcg tagatggtgg tttgcgccaa
    13381 agccgccgat cccacctggt gatgcagtaa cgactaaacc tggtttatga tcccaaacac
    13441 tgtgtccata aggtcttgag cctacatcta aagcattttt caatactgct ggcacagaac
    13501 ggttgtattc aggagtaacg aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac
    13561 gagtccattc agcaggagcc tgttctggag tttcgagatc ctcattgtaa aatggcagat
    13621 catcgatttt tacaaataca ctttcgtacc cttcaggaag caatttcgca aaagcttctg
    13681 ctactttttt gttgtaagaa tcttttctta aactaccaat aaaaaatcca attttttttg
    13741 tcatacgatt tttcctcctt caaactatct ataataatct tacaaaagat aagtaactgg
    13801 agcaaataaa aacacttact gacaaaagct acatagaaga tagattactt atttttaaca
    13861 aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg aatttaatca agctgtaaag
    13921 aaaaaaagct ttacagggta aaaaaaaact gttacactac atcttgtgaa taaaacaatg
    13981 gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag
    14041 tccgttttac cgtatcgtcg tagctgattc acgttctcct cgtgatggac gtttcatcga
    14101 aactgtaggt acttacaatc ctttgaaaga ccctgcagaa gtagttttaa aagaagactt
    14161 agttcttgac tggttatcaa aaggtgcaca accttctgac actgtacgta acatcctttc
    14221 aaaagaaggc gttatgcaaa aacaccacga agctaaattc tcaaagaaat aaggtgaccg
    14281 gatatgaaag atttaagcga cttagtctta acaatcgttc gtccgctagt cacttatcct
    14341 gatcaagtca agttagacgt cgtagagtcg aaagactttt acgagtacaa tctaacagtg
    14401 gctcctgaag atattggccg tatcattgga aaacaagggc gtgttgctaa agcaatccgc
    14461 acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat
    14521 gataaggaaa agtaaataaa cgtgctgtac tcgaattttc gagtgcagct tttttgtttt
    14581 ttttattaat tgaagaaatg attatttggc aaaacaagca agaaagcgta tccttatagg
    14641 aggagggagt gaacagccaa tgagaaagaa aatgttattt attccatttg ctctattgtt
    14701 aagcgcttgt agttcggtta ctcaagaatc agacaataca catctatcaa gcagtatatc
    14761 tgaaactgtt accagtccag aaaaaagtag taccaaaacg gcttccacca ctgaacaaac
    14821 aatggctacc tcaaataatg agaaaaagac tgctttagac cagttgaaag aacaacagcc
    14881 aaatgttccc atgcctctag atgtaccagt ctcttcaggc tatctcaata tcgcagcgac
    14941 acatacaaag caaggttatt ctattttata ttacagaaca gatcgtcccc tcgggttgaa
    15001 tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat ctatatcaat atgggtttgc
    15061 ttctagtcaa gaaacaatcc aagttttgca gccatttgag atcgacacaa atggtcagca
    15121 agtggactta ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt caagttttct
    15181 tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg aataatatag aaggccaaga
    15241 tcccttacta ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc taccagcacc
    15301 tgaacaattt ggtaaaatta ctgttgatat gggtgatacg acgaatcgag ccgtagaagt
    15361 aagctggcaa gaaccgaaaa atgcctatac gatcactcat caagatccta tgtctgcgtt
    15421 gaagatggca gtttctatga agaggttgtg aaatcagcgc gctgatctga gcccatcatt
    15481 taactccaag tcacaggaag taatcaaaaa tagcacgccg catttttgca taattttggc
    15541 ttaatacctt aaaagaaaac tagaattgcc tcttcacaca tgctaaaata aaacagacaa
    15601 aaaagaaatg gggatataag ttgactgaat acttgaatgt aggaaaaatc gtaaacacac
    15661 aaggtatcaa aggcgaagtc cgtgttatct caacaacgga tttcccagaa gaacgttaca
    15721 aaaaaggaac ggtgctaaca ctattccaag acggaaaagc acctgtggaa ttaacagtca
    15781 aaagtcatcg caaacataaa aattttgact tattgagttt tgaaggacat ccttctatca
    15841 atgatgtgga aaaatatcgg gatggcatat taaaagtgtc aaaagacaac tctgtagatc
    15901 tagctgaaaa tgaattctat taccatgaga tcatcggtct gcatgtatta gaagatggaa
    15961 ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc
    16021 aacgtccgaa aaaagctgat atattgcttc catatatcgc ttctgtcgtc aaagaagtcg
    16081 atttagaagc tggagtggtc cgtgtagaaa ttccagaagg actgattgac gatgaagatt
    16141 gatgtattga ctttatttcc aagaatgttt gaaggtccta tgggggaatc catcatcggt
    16201 aaggctgtag ataaaggatt attagatatc aatatctcta acttccgcga ttattcagat
    16261 aacaaacatc agacagtaga tgattatcca tacggcggtg gagcaggtat gctgctcaaa
    16321 gtccagccca tctatgataa catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa
    16381 agagtaattc ttcttgaccc agctggtaaa cgattcgatc agaaaatggc agaagaattt
    16441 tcacaagaag aacacttagt attcatttgt ggtcattatg agggatatga tgaacgtatc
    16501 cgcacattag tgacagatga agtttcctta ggagattatg tccttactgg gggagaatta
    16561 ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt agggaatcag
    16621 acttcagctc aaacggattc ttattcaacc ggtttattgg aacatcccca atatactcgc
    16681 ccggcagaat ataaagggat gaaagtacca gaagtattga caaatggaaa tcataaattg
    16741 attgaagaat ggcaattaaa agaatcacta agaaggacct atcaacgtcg gcctgatctt
    16801 ttggaaacac ttgaatggac acctcaaatg acgaaatttt tagaagaaat caagaaagaa
    16861 gaacaagaaa aaacagcaga tgaagccaag tgactttttc taaaatcttc tttacagtag
    16921 gttgataata tgttagtatt ttatggtgag tgcaaaagca cttaactatt acgatattcc
    16981 gctgtgagaa aagctcataa gaatatttgg aataaggaga agaaaaccat gaatccatta
    17041 atcgaagaat taacaaaaga acaattacgt tctgacatcc cagcgttccg ccctggtgac
    17101 actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca gttatttgaa
    17161 ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa cttatacagt acgtaaagtt
    17221 tctaacggag tgggcgttga acgtacattc ccattgcata caccacgtgt tgcaaaaatc
    17281 gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt actacctacg tgcattacac
    17341 ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat cgttatcaga cagagaaaga
    17401 acccttggta ttcaagggtt ctttttttat tatctgtttt ttt
//
LOCUS       DAYYHB010000050        17314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000050 DAYYHB010000000
VERSION     DAYYHB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.051"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..567)
                     /locus_tag="ACHA6B_001708"
     CDS             complement(22..567)
                     /locus_tag="ACHA6B_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8949280.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(579..1271)
                     /locus_tag="ACHA6B_001709"
     CDS             complement(579..1271)
                     /locus_tag="ACHA6B_001709"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8949281.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1290..1574)
                     /gene="macP"
                     /locus_tag="ACHA6B_001710"
     CDS             complement(1290..1574)
                     /gene="macP"
                     /locus_tag="ACHA6B_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8949282.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1587..2150)
                     /locus_tag="ACHA6B_001711"
     CDS             complement(1587..2150)
                     /locus_tag="ACHA6B_001711"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8949283.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2378..3040
                     /locus_tag="ACHA6B_001712"
     CDS             2378..3040
                     /locus_tag="ACHA6B_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8949284.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3164..4360
                     /locus_tag="ACHA6B_001713"
     CDS             3164..4360
                     /locus_tag="ACHA6B_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8949285.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4461..5837)
                     /locus_tag="ACHA6B_001714"
     CDS             complement(4461..5837)
                     /locus_tag="ACHA6B_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8949286.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5874..6741)
                     /locus_tag="ACHA6B_001715"
                     /pseudo
     CDS             complement(5874..6741)
                     /locus_tag="ACHA6B_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6830..7855)
                     /locus_tag="ACHA6B_001716"
     CDS             complement(6830..7855)
                     /locus_tag="ACHA6B_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8949287.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7873..8547)
                     /locus_tag="ACHA6B_001717"
     CDS             complement(7873..8547)
                     /locus_tag="ACHA6B_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8949288.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8731..9624)
                     /locus_tag="ACHA6B_001718"
     CDS             complement(8731..9624)
                     /locus_tag="ACHA6B_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8949289.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9698..11395)
                     /locus_tag="ACHA6B_001719"
     CDS             complement(9698..11395)
                     /locus_tag="ACHA6B_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8949290.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11388..12701)
                     /locus_tag="ACHA6B_001720"
     CDS             complement(11388..12701)
                     /locus_tag="ACHA6B_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8949291.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12701..16642)
                     /locus_tag="ACHA6B_001721"
     CDS             complement(12701..16642)
                     /locus_tag="ACHA6B_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8949292.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16751..17209)
                     /locus_tag="ACHA6B_001722"
     CDS             complement(16751..17209)
                     /locus_tag="ACHA6B_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8949293.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4856 a         3893 c         3011 g         5554 t
ORIGIN      
        1 ttatcaaatc actttgtttt tttataaaat ttctgcgcct agtgtggact caaaatgtct
       61 caatgcccat tcatgtccaa cgggatcgaa acttgccacg gaatctttga aaatatggag
      121 tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac
      181 gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc
      241 actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc
      301 ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg
      361 cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc
      421 aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc
      481 aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt
      541 gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat
      601 tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt
      661 gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac
      721 tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa
      781 ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc
      841 aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa
      901 tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt gtagccaaat
      961 cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt
     1021 aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt
     1081 tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg
     1141 atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat
     1201 aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata
     1261 ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca
     1321 cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc
     1381 ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga
     1441 taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt
     1501 tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc
     1561 ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga
     1621 atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg ttgcgcctct
     1681 tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg
     1741 accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa
     1801 agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat
     1861 tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt tccagctgga
     1921 atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct
     1981 tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc tctcttcgaa
     2041 gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag
     2101 atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct
     2161 gctttctttg taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt
     2221 attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc
     2281 cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa
     2341 ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg
     2401 gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct
     2461 aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa
     2521 aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa
     2581 aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa
     2641 tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct
     2701 ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa
     2761 ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac
     2821 aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact
     2881 agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa
     2941 attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt
     3001 aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa
     3061 gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat
     3121 tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac
     3181 tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt
     3241 ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga
     3301 gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa
     3361 acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc
     3421 tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga
     3481 tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc
     3541 tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca
     3601 atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc
     3661 agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct
     3721 ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt
     3781 gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac
     3841 tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga
     3901 taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat
     3961 ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac
     4021 agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga
     4081 tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc
     4141 agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga
     4201 tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg
     4261 tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga
     4321 agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact
     4381 aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct
     4441 tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca
     4501 aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc
     4561 agacgaagca tcaatacaat acaagtcaaa atggagacga ttggcaatag cggaacgaat
     4621 ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct
     4681 agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc
     4741 aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc
     4801 ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg
     4861 acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt
     4921 aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca
     4981 aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc
     5041 gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca
     5101 tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa
     5161 gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc
     5221 tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca
     5281 aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg
     5341 atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc
     5401 ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct
     5461 tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc
     5521 aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat
     5581 ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg
     5641 acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc
     5701 gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc
     5761 aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc
     5821 ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact
     5881 gttttcatat accctaaaag ggataccacc taaaatgact aaaaggcaac caacaataac
     5941 ataaatgagc tcttttttcg tttttctttc gccaaggatg aaaatcccac ctaaagtaga
     6001 gataatgatc cccatttgtg aaagtgagaa gccaacagct aaaccaagtg atttgactgt
     6061 tagaagcatg cagatgtttc ctaagcccca taataatcca cagatcatgt ttttccacac
     6121 aaactgatcg actttaactt tacggaaagc aaagaagctt gcgcctaaga tcattccgat
     6181 actttgaggt aagataattg acatcgcatc caaattcgcc cagttgacga tgatggtata
     6241 tacaccataa ccaatcgtag agaagatcaa tgctctaaaa cccttaccaa aatcaagcat
     6301 tttattatct gtatctattt ttccttcgtc atcttgtcgg gcagtcaaat acgcaccgct
     6361 tacaagaaga gctaaagcaa taataccgta tacataatct ttcgtttgtg tccattcatg
     6421 gaagaaaacg gctcccgcaa tcgtattcaa aattaattga aaaccagtag acaatggtag
     6481 ccctacagaa acacccatgt acttcattcc atggaactgt ccattttgtc caacactcca
     6541 agctaagccg gataaaaatc cgatcaagaa aatccaaggg gtgattacag gactaacaac
     6601 gaaaaagaca actaaagaaa acagaaatgc accgatcgtc attcctagtg tttgttgatt
     6661 ggcatcgcca ccaattttac cacttactaa accaatacta ccccatgcca acatagggac
     6721 taatgctatt aaaatttcca taaccaatct ccttttattt cacatgataa gaaacttatc
     6781 agcgaaaacg ttaacaacac aagcactttt tttcgtttct aacgattttc taacgttttt
     6841 cattataaaa atttaagatt gggaaaaata aagtcattgt tgtccctttt ccttcagttg
     6901 attcagccgt caaatggata cctaattttt cggccagatt ttttgctaaa taaagtccta
     6961 atcctgttga atgtgttttg ctaagtcgac catttctccc agtaaatccc ttatcaaaga
     7021 tccgtttcaa gtcttccgct gggataccga tccctgaatc tttaactgaa aggctgactc
     7081 cttgcgcttc cttttctatg ctcacaatga tgtttccgtg atccggcgta tatttgatgg
     7141 cattactaag caattggttg aagataaaag cgatccattt agcatcggtc aaaacagtat
     7201 gatcctcacc aacgatctcg tagcgaatat ttttttggat aaaataattt gcctgactgc
     7261 gtatcacagg ctgaatgatt ccttttagtc catattcttg gatgaggtag tcccgcgaga
     7321 aactgtcgag tcttgcataa tacaaaactt gttctacgta ttcatcgatt tttgataatt
     7381 cattttctag taatgtatac ttttgatcgt caatatcgaa ctcgatcgaa cgaactagca
     7441 atttagaagc ggaaagtggc actttgattt catggaccca tgagtcaata taatcttttt
     7501 ggtcttgttg attactgatt gctgattgca ttacttcttg gtgttcacgg atcagctgat
     7561 tgatatattc ttctaccagt ttttcttctt ctgtcctccc accactcaaa taattttgca
     7621 aaggggaagt tttttgtatg tcggctaatt ttccccacca tcttctcttg ctcaagtaac
     7681 cacccagcag ataaaaaatc agaaagacgc cctcaatcaa agctaaataa gcaattgtgc
     7741 tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa gatcgtcaat gcaataaaaa
     7801 acagccatcc caagatcaat gtccactgat ctttcaaata tttaaaaaaa ttcatcttaa
     7861 ccgctcatcc tttcagggaa cgatatatcc ctgtcctact tttgtttcga tatatccttg
     7921 gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg ttgactgtca atgtattgtc
     7981 atctacaaat cgttcgtcat cccacagtgc tcgcagcaat ttttcccgac ttaagatctt
     8041 tccattgtgc ttcatcaaaa taaataatag acgatattca tttttgctca aatcaatgat
     8101 ctctccgttg atttccatac ttccgttgtc aacattcaac gtaatgccat tgtggctcat
     8161 catttcacta gataattctg tgtagttgta agaacggcga agtaatgcat tgatttttgc
     8221 aatcagtaca tcgatttgga aatgttttgt cacaaaatca tctgccccca tgttcatagc
     8281 catgatcata tccatattcg tattcctgct ggagataaaa atgatcggta ctttcgatac
     8341 ttcacgtatt ttttgacacc aataatagcc atccaaaacg ggaagattaa tatctagtaa
     8401 tacaagctgt ggttcttctc tttcaaagtc tgagaacact tgattgaaat cggtcgttcc
     8461 aaaggtttca aattgccatt tctcaagttc ctccatgatc aattgtcgaa tgatctcttc
     8521 gtcttcgact accatgattt ttgccataaa aaatccctcc atctttcttc ttgctactac
     8581 aatatcaaag aagcccttgt gaaacaagtt ggcttacctg gaaaatgtca ttttagcaaa
     8641 cgttaaaaaa aactggaaat atgcgattta atcgcgtttg tctcttccaa gcagcttaaa
     8701 tttattatgt tattcttaat aattggtcta ttatctgtta tttttctttc gttgtttttg
     8761 tcttttaaat tttcgataac accaataacc gaaaagcgtt agcactagca atatacctag
     8821 aaggagtata agtaatcgat aaagatggta tttttttgtc tgttctatct ttgaagagac
     8881 ttcttctgta aagggaactc gtcttcctgt cactagtaat cggtgagtgt tcacggtgta
     8941 aggtgtacaa gtgataagtg tgactagatc ctgcttttct ctaattgcta atgtatttgt
     9001 ttttgttgga aggacaacaa ttttctcaac gacttcataa gcgagcatcc gaccatacac
     9061 ttcaagatag aatcggtcat cttgctccat ttcttcaaga tccgtaaaga gttttttctc
     9121 agcaataccg ctatgggcgg taatcacgga atgtgtactc tctcctccag ttggaaaaga
     9181 ggtgccttgc aggactgtcg ctcctctatc taacaaaaga tcattcgttt catcaaataa
     9241 aggcaagcta acattgattt tcggaatata gatggctccg atcatatgtt gttcgtaata
     9301 ttcttttcct ggattttgga catttctaac cgcttgctca aaaggatctt tgacttgtcc
     9361 cattcctggg attactgatg tatgagcaag cgtctgattt ttcttttcat gcactgctaa
     9421 ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc ttttgataat tctcgatcct
     9481 tctttgatcc aaataattgc tgagagcatc tgctaaaaat ggatatgtaa aaactgcagc
     9541 tccggcaaga aacatcactg ccataaataa ttgtaataaa agactgattt gggtttgagg
     9601 tctattacct attttctttt tcattttctt caccttctta tttaaaaaaa ctgcagcaac
     9661 ctttataaaa ccgattgctg cagcttattt ttgttgctta tacttcagac ttcattttag
     9721 atttacggta ccagctgtac gctccaatca tcaaacttat tccgattagt aagaatgcca
     9781 agagcccgtt cccaccagta gcaggaagtg acccgcgttt catattcggt actctttctg
     9841 gccctggatt ttctactcct acgatctgaa tttcttcatt atagcttcca taagagatga
     9901 taaattcagt aaaggctgcc tcacctgttg gaagtacata accttctggc gccatggtct
     9961 ctttcatttg ataggttcca taatctaacc cttgaacctc aaagtatcca tttccatctg
    10021 cgacaaattc tgttgcttgt ccgcttgttg cccactcaat tctatctgct cctgattcat
    10081 aaacagctaa aggcgactcc cctttataaa atattgcgta ttcagaacgg tcttcattcc
    10141 acaattcgaa tcttgcatct ttaagcaatt cactgcttga tgcatcatat ttttcaaact
    10201 ttcttccacc tgttgtgact ttctctggcg gctctactcc ttcttcataa tcaaattcat
    10261 catttcgtcc gatttcaata accgctgtgt tgttatggaa atcatccgct tctagttctg
    10321 tggaaacctt caaattatac gtgatcgtta aatctcctcc acgtgcatct gctaatctac
    10381 ctaaagtatc tactttggct actccatttt cttctgtcaa aatcgtcacg acaaacccgt
    10441 tgcttactac ttctactata taatcttgtc cttttgttaa aatcgtctcg ccggctcgca
    10501 cttctatttc ctgatcataa taagttagac caactgtagg cgtatctgtg attttgtaat
    10561 ttttgataac agcgcttccg ttttcaagga cagaatcgat gaaataagga ataggtgctg
    10621 tgatcgtgta agaagccgtt tttcctcttt ctagatcaat ataagaaatc gtttcttgtt
    10681 ctccagcttc attaataatc gttacttgtc taaggtcgct ttcctctaca ttcatctgtt
    10741 tatctgcttc tctgatttcg tttttcggat acaaatgcac atctgtattg tatgtgtctt
    10801 tccatgtaga accatcccac acgccatcca ccatatcagg catcatcact ggcatcataa
    10861 caaccattgg agacgcgatt ctggaaatat gagctggcga ataagtttct aagaaaagat
    10921 agactttatc tctagaagaa ggtgcttctt ccgtcacttg gacggtagag aataccgctt
    10981 ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag tcgatatttt aaaaaccaac
    11041 tttctgtgtg ccgtttttga atccattcaa tcgcttcggc agaattaaga ccgtcttctc
    11101 gttccggtgt taaaggatta tctcgaatca aatcgtaata tacttccgtt acgtcaaaca
    11161 aatcaaaatc gactcctgga atattttctt caccaaaatc cgtagaaacc aatccgctat
    11221 tcggtcgttc ttccggaaca gatgagaaac cttttttgtg taatgtaatc gtcacttcac
    11281 ttggtttttg tgcgacttct tcggctttga ctagttttcc gttcccgaaa atccctattc
    11341 ccgcagtaac gagtaacagt acactcattc ccgtgacaat cattttttta ttcatttttt
    11401 tctccccgtt ctttttttat taataaaaca gcacctaaca ataaaagaat tcctagtaaa
    11461 gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg accattttcc tgattgacct
    11521 ggattgcttg gaccactcgg ctctttcggt ttgtccgttg tttcttttac ggtctcgtta
    11581 taaacacgtg gtgttgcgga ttcataggca gagactggaa ctttcccgga ttctaattca
    11641 gccatctcct gtccgttaac tactacatat tgtggttggc cttttgcatc cacccaaaat
    11701 tcagggatga tgacctcgat tctcttactt gcttcatcca tctcgtaacc ttccacacct
    11761 tttaattctt caaaataata ggtgccagaa ggcaagaaac gttgtcctgt cgtaacaagg
    11821 ccctctttat ccgaaatgaa tttgcttaca tttttgtcat tcaaaggatc attgtctgct
    11881 ggtttgatcc attgattttt caagtcatta gcaggagcca tatctaaata atatttcccc
    11941 ccttctatca tctgatatag agcaaaaaca gcaccttcta atggttttcc tttttcagtt
    12001 gttcctcttt gacgtccata cttgaagaag tatggatctc ttagataacc gacattttta
    12061 ggatagatat gaatctcttg cagttcctcg gttgattctg tcgggtgata aattggcaaa
    12121 atagcagcaa ttgggatagc tgtctgatcg atatcaacat ccaatccaac ttctggatca
    12181 atcgattctt cgaaaatcaa ataaactgca tcccgatctt ggtttttctt aggtgctttg
    12241 atacgtgcaa tcccgtctcc taatccgcca accgtatctt tcccagttgt taccgaagtc
    12301 cctatttgga caagcctttc ttctgtgatt agctgacgga tattgtttct gttcgtttca
    12361 gcgatttgct ggacgaattc tttaggagtc atgttttcag cagctttttt ttcatgataa
    12421 taatcggtca tgtcgtaaat ggcaaatgtc gctccattta gtggcaccgt ctgtgaaatg
    12481 atatctgctg tttcccctgc ttcctcagag atagccaagc catcattttc atggtattca
    12541 aattgatcgt tataatcgat atctcgaatc atccgtttat gcaacacgaa ttccacgaca
    12601 tcttcccctg attcttctgc cgacatcgca tgtggaagaa ggactgctgc aagcggtagt
    12661 aaaacaagta attgaagcat caaacacaac agctttttca ttaccttgct ccctcctttt
    12721 tagattgtct gacaaacaaa ccataggctg ctcctgcaag cgccatgaag aatgcagcaa
    12781 gcaggatata gaataatcgt ccggatcctc ctgtagcagg caacggattt tcaggatcgt
    12841 tcccgacact aagctgtaat ctgattcgat ttgtctccaa aagttcatat tctaactctt
    12901 gctgtagatc aggcgtctct gcttcttcgt ttggataata cgaaatctca acttttagtg
    12961 tacccgcttc ttttcctgga actacccgat cattttctat gtcaggttct tctcgataac
    13021 taatttctaa aataaagtaa cctggaagtt ttctatagct gtcaggtgac ttcacttcct
    13081 tgatccgata gaccccttcc aacaagtctg aagcattgaa tagggaagaa tcgtttggat
    13141 ctggtgccaa gcctccctcg atgatttcat actctcctgc actattttgt ttctcaattg
    13201 aaaattctgc gccttcaaga cgtcggctat tcatactgtc aattttcaaa agttccaaat
    13261 cgattggcgc tagttcatct tctaaggtta cttgtagtac accatcgatg atttcatagc
    13321 cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc ttcaatgata aacttacctt
    13381 cttcattcag tgcaaactgg aacacttgat cagatagccg atacccattt ggtgcgacag
    13441 attccgagac ctcatattca ccaatagaca gatgcagagg ttggtcgtaa tttaaatcga
    13501 catacttttc tactcctacg gcacgagaat agaagtatcc gatcccaggc aaacgtttcg
    13561 attcttctgg ttctaacgga ttaccagtgt atgctcgata cagcgggaag gaagaatccc
    13621 ccactttcct gatatcgaaa cctgcaccat ctaatgtatg gccttcatgg tctaattttc
    13681 taactcggat ctcccgataa tcaaacgtat tgtaagcagt gaatgctacc cataaacgat
    13741 cctcttcatc tgttgtatac gtgagatatt gccaattttc ttcacctgtt tgcttgagtt
    13801 ggatagtccc tatagcatta tcgtaaagtc gcaagatcca tgtgtcattt tcatcaggtt
    13861 ctttgaaacc ttctgggttc tggctctcaa tgattttgta ttctgcatag ttggtagttt
    13921 cttcactatc cttataaaca aatgaatctg gctctccgat tggatgaata actttctcac
    13981 catccacgat tacatatttc ccgtcttcat ctactgaata gaacgtgagt ctccctttct
    14041 cttcctctac ttcgttcaca ccagaaagcc ctttcgctac tggatatccg atttccagtc
    14101 cgttaccatc tgtttcataa agttcaaact ctgcgccatt gattggcata ttactgcctt
    14161 caattagttt attgatattc atgtggattg gcttgaattc atttcgcatt tccagtttga
    14221 catacaactc atcatactct gtgccttcta tgttaggctc ttgtacaaca gaaagcttgt
    14281 gatattctcc ttcataatag gaattcggat ctttaccttc cggtccgccg actctcgaac
    14341 cagatgtata ttcatctaca agctcgtagt gacctaacat ctcatcttcg ttggctgcat
    14401 attttttgat ttggaattga tacgtctcat ctactaactg ataacgtggt ggtccagcga
    14461 tctctctgat ccaaaatatg tcgctgactg ctccagtttc atctcctaaa atcggcatct
    14521 tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact tgtttcgatt ggttcggctt
    14581 ccccactctc tggattttgg tcatataatg caaattcgac tccagccaac ggctcgccat
    14641 tttgaccctt cttgctgact tggaaattac ggcctttatg ttcgtaacga atggaagaag
    14701 atgggaaata catccgtcca gtagtgatat cattctcata atcctccagg ttctgaatat
    14761 aggccatatt gttcactgtg caaaaatttc cgtcttgagc tcccgcatca agttttactt
    14821 ggtacttgat cctgaccatt tccccttcac ctaaagaaat cggagaaaat ttataagctt
    14881 ggttttcccg tccgacatac gttgtcggag tcgccactac ttctggtggg gcttgtccat
    14941 gacgttcttc atcccatatt tctggctctt ggtacttcat gccgttttct cctggatcta
    15001 gatggtaaaa ctgaatatct gccgatctgc cagctccaaa tggtgagaca ttaaaaccag
    15061 tttcatcata aagtgtggct tgctggatcg ttctcttgaa ggtagaagag atatcctcca
    15121 aggctgtaac aatgtcattc ttgttgttcg cattgtaata tcgcggtgtt acgccatctt
    15181 cttctaaatc agcaatattt ttcaatacat ttcgtccaat agcatctagt cgttgtctcc
    15241 cgctaacgtt tccatcgatt cctaaaccaa tggagaagat acttggcggg ttctcttgat
    15301 acttgttttt cagccaatga tgataagcaa ctgtgttgat cgcagatgac ttttcttgcg
    15361 aagtaggact gccattcgta ttgttattag aagaaccatc accaacccag tgagtcgctt
    15421 tattatctcc ttcttttacc tctccataac gatagtgtac atttacttca tcttctggac
    15481 ggttaaagtc agatgcatag gtaactggat gagtaaaccc tgcattagaa gtattcccag
    15541 aaaactcctc aaaatttctg aacaaaacgt cattatcttc agcaatctta tcttccatca
    15601 cagtccagtt gctccaagct ccattagtgc tcgtacgata acttgactga atcggtgcat
    15661 aactgaaggt cggtgtccca tctcccacaa cgattaggat tttttctgga tttctatttt
    15721 ctccgccatt gtctttatac aatgtttcat agccattttt aagccctagt gtaagcggag
    15781 ttcctccgat tggtgtacga tttaatgctg tattctcact tcccgtcaaa cgattgatat
    15841 catttgtcaa tggaaagatg tccgaactga tttgctcatg gttattgata gctgtactat
    15901 tatggtaaaa attcaccata ccgattcgaa tatccagact cgggtcagag agcagatctt
    15961 tactcatttc gtttactgcc tcaatcaacg cagcattttt agtttgacta ttggtcccag
    16021 cggttaattc actcatactc gctgacttat ctagaacaaa aacgatatcg attggactga
    16081 tttccttttt ctcgcccccg atgacatcta gatagacatt gtaaagggtt gttgcatctt
    16141 caggatgtag atcagatgct gtgctgcttg gcagaacagg ttctacccat tttttgatgt
    16201 acgccccgtt ataatccaag taactgtttt caaaattccg ttcatttccc caaagctgaa
    16261 cggtttgagc tggaacgttt ctatcttctt cgcctgcagt tgatccatta ttagtagcat
    16321 agtcgtaatt tcgccagttc gctcccccgt ttgtataatc actatcatcg atcaagttct
    16381 ctccgtcata tggcgagcct ttgtctcctt gatagcgttc attgctttgt gtccaatttc
    16441 ctaacacttt tgtatagtca ttcgttgtaa acttcgggta gcgatgctgt tcgttttcaa
    16501 cttctccgct aggatagata tattcatagg ggtcttgcgt agctgttggc ccaagacgct
    16561 ccaaaatacc gttgaataac tggcgttgaa tgcgctggag tgcttgaccg ctgggtacga
    16621 ttggttcgtc cagcttttcc atctcatttt cttcgctacg ttgaatttca gcggaatctt
    16681 ctgtatcagt ttcattttct tgtaattctt ggttatctat tctttcatca accaccactt
    16741 gaagaggtag ttacccttcg ttcacatcgg ggatcttttc tgtttcaaaa atcaatgtgc
    16801 cggtttctgc ttcttttgaa taaacttttc cgacatacca ttcttgacca tcgatcgtct
    16861 cgacttttag gtcttctcca tcttcatcat aggaggatag acctgttcca cgaacgtttt
    16921 ttacggtacc aatcccggcg gctgccgcat cgattgctaa acgtattcta ccttcattgg
    16981 cactttcctt tttttcaaaa gccagatgcc attcaagggt ttcttctttt tcaatatact
    17041 tcccttcaat ttttgcatag tcctcatcta agaatacctc atcctccttt gtttctgttg
    17101 actcagcagc tacatccagg gtcgtcatat gatagactga ctggaaatat ggtccaaata
    17161 gcaaactgac agttactaac tgcaaaagct tcaattttaa ccctttcaca tccttatact
    17221 cctttcaaaa atatttgtct tctttcacaa caatcaacaa aacaatgccc atctaattat
    17281 aatttaattt aattttaatt ttaaatcaaa tgat
//
LOCUS       DAYYHB010000051        17035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000051 DAYYHB010000000
VERSION     DAYYHB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.052"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACHA6B_001723"
     CDS             complement(26..328)
                     /locus_tag="ACHA6B_001723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949294.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACHA6B_001724"
     CDS             complement(353..2446)
                     /locus_tag="ACHA6B_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8949295.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACHA6B_001725"
     CDS             complement(2935..3699)
                     /locus_tag="ACHA6B_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8949296.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACHA6B_001726"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACHA6B_001726"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8949297.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACHA6B_001727"
     CDS             complement(4704..5780)
                     /locus_tag="ACHA6B_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8949298.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACHA6B_001728"
     CDS             complement(5804..6397)
                     /locus_tag="ACHA6B_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8949299.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACHA6B_001729"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACHA6B_001729"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8949300.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACHA6B_001730"
     CDS             complement(8067..8366)
                     /locus_tag="ACHA6B_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8949301.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACHA6B_001731"
                     /pseudo
     CDS             complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACHA6B_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10126..11037)
                     /locus_tag="ACHA6B_001732"
     CDS             complement(10126..11037)
                     /locus_tag="ACHA6B_001732"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8949302.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11157..12014
                     /locus_tag="ACHA6B_001733"
     CDS             11157..12014
                     /locus_tag="ACHA6B_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8949303.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12201..12563)
                     /locus_tag="ACHA6B_001734"
     CDS             complement(12201..12563)
                     /locus_tag="ACHA6B_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8949304.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12752..12934)
                     /locus_tag="ACHA6B_001735"
     CDS             complement(12752..12934)
                     /locus_tag="ACHA6B_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949305.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12909..14348
                     /locus_tag="ACHA6B_001736"
     CDS             12909..14348
                     /locus_tag="ACHA6B_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949306.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14355..16907)
                     /locus_tag="ACHA6B_001737"
     CDS             complement(14355..16907)
                     /locus_tag="ACHA6B_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8949307.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT         4805 a         3625 c         2946 g         5659 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tgcattggct aagcctgctt gtttttgaac
     8821 agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
     8881 catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg
     8941 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc
     9001 aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa
     9061 ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt
     9121 actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt
     9181 ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc
     9241 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
     9301 tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc
     9361 gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat
     9421 tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
     9481 aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
     9541 agaatcgaca acttcgttaa cttgaacgat cacgatacca tctttgaaag ctgcagcttc
     9601 gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc
     9661 tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag
     9721 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
     9781 acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa
     9841 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
     9901 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
     9961 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    10021 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    10081 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    10141 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    10201 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    10261 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    10321 agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    10381 cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca tatacggctt
    10441 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    10501 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    10561 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    10621 aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac
    10681 gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag
    10741 ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat
    10801 cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa
    10861 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    10921 atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    10981 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    11041 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    11101 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    11161 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    11221 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    11281 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    11341 aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat
    11401 tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg
    11461 tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga
    11521 tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga
    11581 tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta
    11641 agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca
    11701 tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga
    11761 aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg
    11821 tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg
    11881 atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt
    11941 ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt
    12001 atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga
    12061 acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga
    12121 gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc
    12181 ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct
    12241 agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg
    12301 tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc
    12361 ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct
    12421 ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa
    12481 tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg
    12541 attagttctt ttttcgttag catctgcttc catctctttc ttattgattc gctttattca
    12601 aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa tacttcgata
    12661 tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct
    12721 actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt
    12781 tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg
    12841 aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag
    12901 tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg
    12961 tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa
    13021 tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc
    13081 ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt
    13141 tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga
    13201 aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt
    13261 cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact
    13321 tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta
    13381 ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt
    13441 cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg
    13501 ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag
    13561 cgaattgccg attagattct tctatccata ccaacagcac actctcctcc ctgtatgact
    13621 ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt
    13681 ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg
    13741 ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag
    13801 cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc
    13861 tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc
    13921 gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac
    13981 tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt
    14041 ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
    14101 ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag
    14161 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa
    14221 aaacagatat cttgatttcc aatttccctc tagattctct agatatccct attgtataca
    14281 tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
    14341 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
    14401 ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
    14461 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
    14521 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
    14581 ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
    14641 acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca
    14701 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
    14761 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
    14821 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
    14881 acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc
    14941 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
    15001 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
    15061 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct
    15121 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt
    15181 tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca
    15241 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
    15301 atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
    15361 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt
    15421 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
    15481 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
    15541 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
    15601 tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc
    15661 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
    15721 atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca
    15781 acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc
    15841 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
    15901 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
    15961 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
    16021 ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
    16081 aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
    16141 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
    16201 atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta
    16261 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
    16321 agcgttcctg aaaaacccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
    16381 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
    16441 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
    16501 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
    16561 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
    16621 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
    16681 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca
    16741 gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
    16801 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
    16861 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
    16921 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
    16981 gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata tttct
//
LOCUS       DAYYHB010000052        16528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000052 DAYYHB010000000
VERSION     DAYYHB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.053"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..1172)
                     /gene="rpoD"
                     /locus_tag="ACHA6B_001738"
     CDS             complement(63..1172)
                     /gene="rpoD"
                     /locus_tag="ACHA6B_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8949308.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1196..3037)
                     /gene="dnaG"
                     /locus_tag="ACHA6B_001739"
     CDS             complement(1196..3037)
                     /gene="dnaG"
                     /locus_tag="ACHA6B_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8949309.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3219..5534)
                     /locus_tag="ACHA6B_001740"
     CDS             complement(3219..5534)
                     /locus_tag="ACHA6B_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8949310.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5719..6579)
                     /locus_tag="ACHA6B_001741"
     CDS             complement(5719..6579)
                     /locus_tag="ACHA6B_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8949311.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6638..7270)
                     /locus_tag="ACHA6B_001742"
     CDS             complement(6638..7270)
                     /locus_tag="ACHA6B_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8949312.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7437..8198
                     /locus_tag="ACHA6B_001743"
     CDS             7437..8198
                     /locus_tag="ACHA6B_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949313.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8224..10815
                     /gene="mprF"
                     /locus_tag="ACHA6B_001744"
     CDS             8224..10815
                     /gene="mprF"
                     /locus_tag="ACHA6B_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8949314.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..12190)
                     /gene="tuf"
                     /locus_tag="ACHA6B_001745"
     CDS             complement(11003..12190)
                     /gene="tuf"
                     /locus_tag="ACHA6B_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8949315.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12558..12914
                     /locus_tag="ACHA6B_001746"
     CDS             12558..12914
                     /locus_tag="ACHA6B_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8949316.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13155..14159)
                     /locus_tag="ACHA6B_001747"
     CDS             complement(13155..14159)
                     /locus_tag="ACHA6B_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8949317.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14325..15176
                     /locus_tag="ACHA6B_001748"
     CDS             14325..15176
                     /locus_tag="ACHA6B_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8949318.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15193..15438
                     /locus_tag="ACHA6B_001749"
     CDS             15193..15438
                     /locus_tag="ACHA6B_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949319.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            15625..16329
                     /locus_tag="ACHA6B_001750"
     CDS             15625..16329
                     /locus_tag="ACHA6B_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949320.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         4857 a         3409 c         2927 g         5335 t
ORIGIN      
        1 ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc aatagtccac
       61 gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa ttttcttaat
      121 gctttagcct cgatctgacg aatacgttcc cgagtaacac caaacacttt ccctacttct
      181 tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac attctcttcc
      241 cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc ataagctgcg
      301 tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg agagtcatct
      361 tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa gatttctcgg
      421 actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg ttctcttcca
      481 aggtcttgca gcaattgacg ttggatacgt atcaatttat taattgtttc aaccatatga
      541 actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc ttgacgaatc
      601 caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt ttcaactgcc
      661 ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc tcgtcctaca
      721 taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg ctgtttcgct
      781 tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc agttaaaagg
      841 gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt tacaccggtt
      901 ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact cttcaagctg
      961 tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc aatcttttgg
     1021 atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg attggataaa
     1081 tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc tactgctgct
     1141 tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg agtagctatt
     1201 tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca agctcaagct
     1261 gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt atttcatcag
     1321 ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc gaactttctt
     1381 ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca tcttgcaaaa
     1441 agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca tcgaataaaa
     1501 aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt aaaatattac
     1561 ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt tctacttgcg
     1621 tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct
     1681 gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg
     1741 tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct tgttcgattg
     1801 gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc ttgtcttttt
     1861 cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag actgtttcgc
     1921 gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga tattcatcag
     1981 gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt tgccgaagca
     2041 attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca
     2101 attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt gtccccatag
     2161 aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata aacccttcga
     2221 acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga ttaaataaaa
     2281 cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc tgatcttttt
     2341 cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat
     2401 tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca tcatcatgcg
     2461 tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc ttgaatactt
     2521 gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca attaattcta
     2581 aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt gtatgcaata
     2641 gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc cccgtagcag
     2701 attcaggatt ttggacagct atagtacgct gacgccattg atcttctaac ggtatctgtt
     2761 cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt tcttgaagaa
     2821 aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg
     2881 caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag taatttttac
     2941 cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt tgactgcgta
     3001 ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct gctctattga
     3061 tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa tcgcactaac
     3121 tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc atatttcatt
     3181 gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac tcttttcagt
     3241 aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt tacgagcatg
     3301 aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa aattccatgg
     3361 aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat
     3421 gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat cagcaagtaa
     3481 tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag tagatacacc
     3541 gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag tgctgtaggc
     3601 aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa ggaaggatac
     3661 ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa tccaattgct
     3721 gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa aaagatgcct
     3781 gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc tggcaatgta
     3841 gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc atcaccttct
     3901 gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac accgtcgcct
     3961 gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt cttttgctgc
     4021 ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc catttcttca
     4081 gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg tctagcgata
     4141 ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc ttcctcatga
     4201 agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc aaacagagca
     4261 accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc gatccccaac
     4321 ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag ccgatcttct
     4381 tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa ttccatcgcg
     4441 ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg attagaaagc
     4501 tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc tgccagatac
     4561 gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc tacccgctgg
     4621 atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt atcgacatgg
     4681 gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc cagtttggtt
     4741 acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag catgcctaac
     4801 aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat gaacagtgct
     4861 tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt ggatactttg
     4921 cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc gctagtcaag
     4981 cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga accgcttgtc
     5041 aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc agattcgcct
     5101 gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc ggctggtatc
     5161 tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt agaaatcgat
     5221 tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga taatttccgt
     5281 acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac gacaataatt
     5341 gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc aataccaaag
     5401 ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt actttttgat
     5461 gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac ctcttgctgt
     5521 gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat aatcacgtgt
     5581 ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt atagaaaaac
     5641 acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt
     5701 tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc accaacctag
     5761 tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc ccaatcccgc
     5821 gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa tgaaatggaa
     5881 tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc caagaacaca
     5941 aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt taaatccgtc
     6001 agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca atcctgggac
     6061 gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat tctgatcgac
     6121 ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa caaaagtaac
     6181 aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt tccataacgc
     6241 aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt catacgcacc
     6301 taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc ctagaccaac
     6361 aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta aaaaagaaat
     6421 cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc ctggcaagat
     6481 aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt tggttgtgta
     6541 ccccacttct tgattttcca ttttttgcct tttttccact atttgcgcac tccttctttc
     6601 atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact ttttcatagg
     6661 tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta cctccattat
     6721 gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg tattgtttcc
     6781 cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct gctaattcta
     6841 ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca aaattatatg
     6901 cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc atttccgcca
     6961 aatatttgac accttgatca atactgtcag ctggattatc aatgatctca gcttctccat
     7021 gcagactttc cgagctccgc atgagatctg tcccttttcc ttttgattcg gtcaatatta
     7081 tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct gttgttattt
     7141 ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcct aaaaacaaca
     7201 ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata gataatcgtt
     7261 ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact atttctttat
     7321 cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg aattttcctg
     7381 tattttttgt catcttacga aaatgatcgt tgtttttcag taaaaacaat tgcccaatga
     7441 ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa aagaaatatg
     7501 caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc ttccctctct
     7561 ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc cctgtactcg
     7621 aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg acaaacgaag
     7681 catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg ttgctggaaa
     7741 aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca ctggtatttg
     7801 ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac acgcttgtta
     7861 aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg gtatggttac
     7921 tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg
     7981 acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg atcatcgtgg
     8041 gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact ataccaaaag
     8101 cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat cctgaacgct
     8161 tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata gaaggtgatt
     8221 tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt caaaacaatc
     8281 tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg taaaactcta
     8341 tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag cggacttatg
     8401 ctgttgattg gtcttgtctc tgtgttacct atgattggtt atgatattat tttgaatcgg
     8461 atgcttgaac aaaagcagaa tcctcgttac ttgtttgaga caagctggct gatcaataca
     8521 atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc tgaactatat
     8581 ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt cttgtttttg
     8641 atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt cacgccagtt
     8701 gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata ttttcctgtc
     8761 gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc acttaaaact
     8821 caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt
     8881 tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc gttattcatt
     8941 gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag tttcgatgta
     9001 atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt tgtgtggatc
     9061 ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt ctttttcttc
     9121 aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca attagcaaca
     9181 gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat gctagtactt
     9241 tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt gaacccattg
     9301 aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt aatcattatg
     9361 ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt cttgattgtt
     9421 ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt tttcttgtct
     9481 attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca attgatctat
     9541 tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt gctttacatc
     9601 gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt tatttccttt
     9661 ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat cgctgttagt
     9721 ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat tggcgaagcc
     9781 tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc cgatagtcaa
     9841 ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga accaactgtt
     9901 cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc ttctggtaag
     9961 aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg actatgctat
    10021 ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga attcggctac
    10081 gatttcatta agatggggga agaagcttat gtagacctga atagttttac aacttcaggg
    10141 aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg atttacattt
    10201 gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa tatctctgat
    10261 aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc tgaagattat
    10321 ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt cgcttttgca
    10381 acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat gcgatatgat
    10441 cctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt tgcctatatg
    10501 aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa tgtcggaact
    10561 tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt tggttctcat
    10621 ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa ctgggtctca
    10681 cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc tttgctgatt
    10741 attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa aaaatggata
    10801 cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg aataaaagag
    10861 actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt gaagaacaaa
    10921 gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat ttttcattac
    10981 caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct gaacccactg
    11041 tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt ggatggatca
    11101 agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct tctggcaatg
    11161 tgattgttcc tgtaacatca gttgtgcgga agtagaattg tggacgatag ttgttgaaga
    11221 atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct ttgaattttg
    11281 tatgtggagt aatggaacct ggtttagcaa gcacttgtcc acgttcgata tcgtcacgtt
    11341 ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct aacgttttac
    11401 ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc cctacgatct
    11461 ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca acagttcctc
    11521 gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct gtatcacgtt
    11581 ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca gcttcagcat
    11641 ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg tcatcgcctg
    11701 ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg atcaattctt
    11761 catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca ccaacttggc
    11821 gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca gaaacaacta
    11881 agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag tccgcgtgtc
    11941 ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg tgtgcggtat
    12001 tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca tagtcttgag
    12061 gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa gtagttttcc
    12121 catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg tcataatgtt
    12181 cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat ttcagctctc
    12241 acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga cttacttttt
    12301 gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact aagagcactc
    12361 gcttgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa
    12421 aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga ggaaaagttt
    12481 aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg ttaataataa
    12541 ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc
    12601 tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat tatacaaaaa
    12661 tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa ttcacgactt
    12721 atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg acgcttcgcg
    12781 aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc aatcctcgta
    12841 aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa
    12901 aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaaagaggc tgggacagaa
    12961 gcgtttaact ccaagaaata agaaggagtt gcttctgctt ccgccgttta tacgtgttta
    13021 gagcgtgaga caaaagtgtt ctttactttt gtctcacgct ctttttttgt tatattcgtc
    13081 ttttccgatc cttctcttga cacaattcat ttaactgatc actggtactt gaacacctaa
    13141 tgttctagtc atttttataa acgattcaat acttcatcca catttttgat catcacggat
    13201 atcgtaccat ccggattatt cacaaattca atcaaatctg ggttgcgata tacatctact
    13261 ggtacgatca gttcgatccc gtttgatagc ttgaattttt gttttccaaa ttttttctca
    13321 gaaatttctt gtacttcttt tacaggaggt gcttctggga caaatccttg ctcttgtact
    13381 tcttctttaa aagccatttt tgccgtgatg ttttccttga agactttttc cgcaatcatc
    13441 tcatgatcca accgaccttt ttcttctatc gtatcataga cagcttcttt caaatcagcc
    13501 atgatatcaa attcttctgt ctcaaatttt tttccaagat gcttggctac tttttggatc
    13561 acttttacat tttcttctaa agaaggtgca ggaacacttt cgatcactct tccagaaaaa
    13621 taaagctctt ttttgccaga aaactcatat tttttttcaa ttaattcgta ggtccaatca
    13681 gtcaaattca cagcgaatgc ttcatctgct ttttgtgttt tcgcccctag aattgctcga
    13741 ttcaaaatca agcggttgtc gatccctgct tcgtctgctt caacaaagtg agtgtaagcc
    13801 tcacgataat tgactttcaa taaagataag tacatcactg tatctaattc atacaaaaca
    13861 acaaatacat ctgcactagg tgcttcttca ctttcttggt agatatcgaa ccaccgctga
    13921 acgattgctt cacttgcttc cacaaaattt tcttgagcga ttttaagtga ttcaacgatc
    13981 atgctttctt ctgctagttg tccagttttt gtttgagcag aagagatttt ttggatcttc
    14041 ttttgaagaa aatcacgaat atattctttt gtcagatcca gctctttttg tgaaaagact
    14101 ggagaaccag tttctctgtc aataatatgc agaattgctt tttttaaata gatatccatt
    14161 ttctcattcc cttctgtctt agtttacgga aaaaaaatca aaaataccag aatcaaaatc
    14221 attttctttt aaacaaaaat ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa
    14281 tgcgctacac ttatagttga acgaataaaa aaggagctga aagtatgaat tcattaacaa
    14341 gcacttatcg tctattaaat ggttacgaaa tcccggtagt aggattcggc acatggcaaa
    14401 cacctgatgg ggatgtcgct gtttcctctg ttaaagaagc gctggctgca ggttatcgcc
    14461 atatcgatac tgcccaagga tacaagaatg aagaaagtgt cggccaagcg attaaggaaa
    14521 gtggaattcc tagagaagag atattcttga caacaaaatt atggaatgca aaccacagct
    14581 atgaacttgt gatgtcttct tttgaagaat cattgaagaa gctccaaaca gattatttag
    14641 atctgttttt gatccactgg ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa
    14701 atgcagatac atggcgagca tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg
    14761 gtgtgagcaa tttcttaccc caccatctag atacactagc aaaaacagca aaaattatgc
    14821 cgatggtcaa tcaagttttc cttgcaccag gcgaattaca gccaactgta gttgagtatg
    14881 caaaaaaaca tgatatgatc ctcgaagcat atagtccatt aggcactggt aaaatcttcg
    14941 atgtgcctga aatgaaacaa atcgctgaag cacatgacaa gacgattgca caagttgctt
    15001 tgcgttggtc gctgcaacac gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa
    15061 tcaaagaaaa tactgaatta tttgattttg aattaacaga agaagaaatg aaacaaatcg
    15121 atcagttaga tggtgtagtc ggcaaagcaa aagacccaga tactacccaa ttttaagtcg
    15181 aaaggtgatt ttatggatcc aatcgaacgc ttgaattctc tctcggaaga agttacccag
    15241 acttttcatt ccgattttgt ctttttgatc gatgcagaga aaatccaaca ttttcctgca
    15301 agaaactgga cacatgatca aatcatcgaa gaactgaaaa aacggttcga tcattcgcta
    15361 atggtcaaac cctggcacga acatgaagtc atttattcac ctgaactacc tgtttttgcc
    15421 ttgataccga aaaaatagaa agaatggaac ggattatgta tatatacata atccgttcca
    15481 ttttttattt ttctttcaaa aaacgggaaa atacgcaaat ttttctgttg taatttgcgt
    15541 atacgcaagt ttttttgcat tactcctttt catttggctg tttttttctt ataatcgaaa
    15601 gggtatgagc gaaggagaga acatatgaag aaaaaacaga aacaaaaatt attaaatcaa
    15661 ttccgtcctt cattggatgg tgtaagagga caattattcc gttctctaga agaagagagc
    15721 ttgcgacgct atggtttgcc gttacaagtc atgcttgatc ccaaaaaccg tcaaatctta
    15781 acgatcggat tagctgggaa aacaaatgaa gatacacgga tcaacgtgcc aatcgatgac
    15841 aacttcaata ctgtattaaa gagaattcgt tcaggagaaa acgggatttt cgaacgattc
    15901 cgtgacaatc ttcttattga aatcgtgtct tactggaatg atcaacgatt gaaaaacgat
    15961 gaaccaactg cgcaagctgt tgcgacacca gttacagaag aaacagtcag tgtgccagaa
    16021 gaaaaaacgg aaaaaccaaa agcggacaca actgaaaata cacgctcaac tgatacgaat
    16081 gccgctttaa gttttgctga tttttcaaaa gccgttgctg agtatcctaa attttacaca
    16141 gaacaaaatg aacatgaagt agttattaat gaaaaaaatg ctgatgaacc tcgtaaatta
    16201 gcaactattt ctatgactac tgaaaatgag ttcgttatcg aaaaagcact cgaacggaaa
    16261 tacaaagtca aattgacttt gattcctttg atcgaacaat ttgcagcgac agcgattact
    16321 gatcgataaa aattctattt gtcataaaag gctgggattt aaaatcccag cctttttagt
    16381 catataataa tttctacagc aagtaaaaag aaattccttc taacatcagg ctcaaatatc
    16441 agcttgacct gtccaacaaa tcacctatga tcctacaatg ttaggcgact tctcttctac
    16501 ctgaagatat cttggagttg cctgattt
//
LOCUS       DAYYHB010000053        16078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000053 DAYYHB010000000
VERSION     DAYYHB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.054"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..1138)
                     /locus_tag="ACHA6B_001751"
     CDS             complement(95..1138)
                     /locus_tag="ACHA6B_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8949321.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1515..2480)
                     /locus_tag="ACHA6B_001752"
     CDS             complement(1515..2480)
                     /locus_tag="ACHA6B_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8949322.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2714..3559
                     /locus_tag="ACHA6B_001753"
     CDS             2714..3559
                     /locus_tag="ACHA6B_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8949323.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3750..4937
                     /locus_tag="ACHA6B_001754"
     CDS             3750..4937
                     /locus_tag="ACHA6B_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8949324.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4943..5299
                     /locus_tag="ACHA6B_001755"
     CDS             4943..5299
                     /locus_tag="ACHA6B_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8949325.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5301..5639
                     /locus_tag="ACHA6B_001756"
     CDS             5301..5639
                     /locus_tag="ACHA6B_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8949326.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5715..5971
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6034..7068
                     /locus_tag="ACHA6B_001757"
     CDS             6034..7068
                     /locus_tag="ACHA6B_001757"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8949327.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7061..7747
                     /locus_tag="ACHA6B_001758"
     CDS             7061..7747
                     /locus_tag="ACHA6B_001758"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8949328.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7771..8619
                     /locus_tag="ACHA6B_001759"
     CDS             7771..8619
                     /locus_tag="ACHA6B_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8949329.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8816..9589
                     /gene="sufC"
                     /locus_tag="ACHA6B_001760"
     CDS             8816..9589
                     /gene="sufC"
                     /locus_tag="ACHA6B_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8949330.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9602..10888
                     /gene="sufD"
                     /locus_tag="ACHA6B_001761"
     CDS             9602..10888
                     /gene="sufD"
                     /locus_tag="ACHA6B_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8949331.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10885..12120
                     /locus_tag="ACHA6B_001762"
     CDS             10885..12120
                     /locus_tag="ACHA6B_001762"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8949332.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12107..12577
                     /gene="sufU"
                     /locus_tag="ACHA6B_001763"
     CDS             12107..12577
                     /gene="sufU"
                     /locus_tag="ACHA6B_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8949333.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12582..13973
                     /gene="sufB"
                     /locus_tag="ACHA6B_001764"
     CDS             12582..13973
                     /gene="sufB"
                     /locus_tag="ACHA6B_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8949334.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14071..15213)
                     /locus_tag="ACHA6B_001765"
     CDS             complement(14071..15213)
                     /locus_tag="ACHA6B_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949335.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15264..16061)
                     /locus_tag="ACHA6B_001766"
     CDS             complement(15264..16061)
                     /locus_tag="ACHA6B_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8949336.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         5220 a         2963 c         3381 g         4514 t
ORIGIN      
        1 ttgttgttgt tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc
       61 atgcgagaat cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata
      121 gtaaagccat cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac
      181 cttctttcat gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct
      241 caaaccagct gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc
      301 tgttgaccaa acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt
      361 ttttctgcgt atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt
      421 caaacagata gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga
      481 attcgacaac agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga
      541 gtgtaccacc caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa
      601 gaaaagggag agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc
      661 ctttttcttc ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac
      721 aaacagcgtt tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac
      781 caatcaagta agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt
      841 ttacaccaat ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga
      901 attctaatag tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa
      961 aagtatttcc taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca
     1021 gataaatagc aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta
     1081 agatttcgcg gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt
     1141 gatcggttcc tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca
     1201 gtattttgac aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa
     1261 taaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
     1321 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct
     1381 tggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat
     1441 gaacagctgt ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc
     1501 tattgaatcg attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct
     1561 ttaattaaga aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga
     1621 cattccgcaa cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa
     1681 tgaacagtag taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag
     1741 cttggataaa aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat
     1801 acttgagata aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta
     1861 agataacctg ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc
     1921 ggatgagcca tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca
     1981 tagcagatca atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca
     2041 gttacagtga tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca
     2101 attgaaagat cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg
     2161 aagataacag cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca
     2221 aaataagtac aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt
     2281 ggatcatgca acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat
     2341 atcataagaa tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct
     2401 ttaaacagat ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata
     2461 attttcaaaa agttcttcat atataatatt ctccttttta tttaatagat tttagtacca
     2521 agatgaacga atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct
     2581 gattgtcttc atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta
     2641 tagatggttt tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta
     2701 tggaagtggt gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa
     2761 tttttccaga gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa
     2821 acagttggag cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct
     2881 tccaactaag caagccgact tattatatga acggacactg gttttgttag aagaatggga
     2941 agaaactcag caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc
     3001 ttcccacacc tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt
     3061 tcctaaagtg cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc
     3121 gaaacatgaa tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag
     3181 atactctttg atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct
     3241 tgtaagagaa gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa
     3301 tatccaagat aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga
     3361 aggaatcggt cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc
     3421 gttgaatttc catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct
     3481 gctagagatt gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga
     3541 ggagaatctc agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag
     3601 ataagcagta ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt
     3661 tttcccaaat gtctatggaa agactttcca ttatctaact gggccgctat aataatcagc
     3721 agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata
     3781 atcgtattga ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct
     3841 cattatatgt agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga
     3901 tgcaagttgt ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt
     3961 ccaaatggat ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac
     4021 tcttgctcaa attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc
     4081 gattcggtgc gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga
     4141 tggcactgat cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact
     4201 tttggctgat tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc
     4261 aagatgactt tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt
     4321 ctggaatctc ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag
     4381 gaacgatctt cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca
     4441 aatcttatca gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa
     4501 tgagttatca gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag
     4561 gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta
     4621 tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa
     4681 tctatcgtat gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa
     4741 gcggtttgat catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat
     4801 tacttccttt aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag
     4861 ggaacatgat cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa
     4921 gatcttcaag gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg
     4981 caaaaaagcc aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat
     5041 caaagaagta ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat
     5101 ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt
     5161 aggcgagttt acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa
     5221 gcgaccactg atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta
     5281 tgaaggagaa ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga
     5341 tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg
     5401 gagacatcac gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc
     5461 tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag
     5521 tttcttctgt taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa
     5581 tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa
     5641 atcagaaaat cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca
     5701 atcctatatg aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag
     5761 agccctgttt gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt
     5821 aagcgagcgt aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa
     5881 gaagctaagt ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg
     5941 taacacggaa gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg
     6001 tttgaaaaat tataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc
     6061 aaaaagacat tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac
     6121 gttgaaaaag gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta
     6181 gtccgtctcc tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa
     6241 gatattacaa aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc
     6301 ttccagcatt tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg
     6361 gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta
     6421 gtcgggttag aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa
     6481 cgcgtcggta tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct
     6541 actagcgcat tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat
     6601 aagaaattga atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc
     6661 tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact
     6721 gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca
     6781 gaagctgatt cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg
     6841 gcaactttga cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga
     6901 cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc
     6961 tttggttcat tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg
     7021 ttcatcaaag aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga
     7081 aacatatcta gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac
     7141 actatatatg acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt
     7201 gatcctttac tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt
     7261 atcgatcatc agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat
     7321 tccatttact aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct
     7381 tgttttatcc gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag
     7441 ccctggtgta ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa
     7501 ggtcttgata cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc
     7561 gatgatcgga tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc
     7621 ttggcaggaa ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat
     7681 tttactaatc gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa
     7741 aagataatag gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca
     7801 gcgcttttgc tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca
     7861 gcaaataaag cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag
     7921 attttggaac atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa
     7981 ttcgatgact atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac
     8041 ttccagcata ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat
     8101 gccggtgctg tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc
     8161 gaattaaaag atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc
     8221 atcacaattc tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca
     8281 gcaacattcg atgatattga agaaaaacct aagaacttga agttcaacca tacgatcgac
     8341 ccatcacttt tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca
     8401 aacttcgctt acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac
     8461 aactctcctt acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc
     8521 aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg
     8581 ggcggctctg ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga
     8641 gaaataagtc tgtaatggct tatttctcat cttttttata cctattctaa tttagaaatc
     8701 aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc
     8761 attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc
     8821 tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt
     8881 aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa
     8941 atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat
     9001 tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt
     9061 atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg
     9121 tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa
     9181 aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct
     9241 gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt
     9301 agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa
     9361 agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac
     9421 ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg
     9481 ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc
     9541 tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag
     9601 aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa
     9661 aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact
     9721 gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga
     9781 aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc
     9841 tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga
     9901 aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca
     9961 agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt
    10021 agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc
    10081 actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt
    10141 gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga
    10201 acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa
    10261 aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg
    10321 tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca
    10381 tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat
    10441 cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc
    10501 ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac
    10561 ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag
    10621 tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga
    10681 tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat
    10741 gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg
    10801 attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga
    10861 agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct
    10921 atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc
    10981 caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat
    11041 gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga
    11101 gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt
    11161 acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga
    11221 gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg
    11281 gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat
    11341 atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta
    11401 tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac
    11461 ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa
    11521 gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt
    11581 ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc
    11641 gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc
    11701 gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg
    11761 caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg
    11821 ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg
    11881 acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta
    11941 gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac
    12001 cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc
    12061 gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa
    12121 attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg
    12181 aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat
    12241 cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg
    12301 ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat
    12361 agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa
    12421 agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg
    12481 gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac
    12541 cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag
    12601 aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct
    12661 tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg
    12721 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
    12781 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
    12841 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
    12901 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
    12961 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
    13021 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
    13081 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
    13141 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
    13201 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
    13261 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
    13321 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
    13381 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
    13441 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
    13501 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
    13561 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
    13621 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
    13681 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
    13741 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
    13801 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
    13861 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
    13921 cagtcgaact caatcgattg atcagttatg agatggaagg gtctgtaggt taataactat
    13981 ttcattgctc gaaacgttga tattaaagcg tttcgagcgt ttttttattt gtattttaat
    14041 ggttggctac gtttttggct acgtttggaa ttattcgccc ataaattttg caaatcggtc
    14101 accagttttc ttaacagatt gtggtgtgac atgagcatag atattcatgg tagttttaat
    14161 atctttatga cctaatcgtt cctgtacttc ttttatcgac gcaccacttt caaacaataa
    14221 gctacagtgg gtatgcctaa atccatgtgg agtaatttga ggctccatcg gtgtcttttt
    14281 gtaaatccag tctaaccaat cgtttactac ttgggggtag tacaacttgt ttgtgtttgt
    14341 agtgaaaaga aactgttttg gtgaattcgt gttatagcca tataagatca ttattttcat
    14401 ttgttcttgt tgccaaatac gcagttgttt aagtgtaaaa tcatcaagct gaatcgttct
    14461 tgatgaattt ttagttttag gtacttgtag aactacttga ttaaactcgt cttgagcgat
    14521 agttttacca attgttattg ttttattgaa atagtctata tcttcccatt gaagcgccaa
    14581 tgcttcgctt ttacgcattc ctgtataagc taataaacgg aaaaaagtgt acagtttaac
    14641 tgaattattt tctttagtat tctgtaaaaa cagttgtaat tgatctttcg tgtaaaactt
    14701 caagggttgt tcttcttcaa tttttcttgg aagcaatgtt ttagacatag gattatcctt
    14761 acagacttcc attgctactc caaatttaaa tatttgagca gttgtttttc tcaagaaacc
    14821 atattgtttt aatgttttgt gccattcatt cacctgtttt tggcaatagc taacacttat
    14881 tttatccaat cgtaattccc caaaagcagg taatatatgg tttttgcaaa aacgttgagc
    14941 aacggcaaca gaagacggtt ttactcttgt tctgtagtta tcaatccata attcatacaa
    15001 ctcttgaaaa gttgtaacat tgattttaaa ctcttgtttt tctttaaagt ctacaagtag
    15061 tctattaagt gctgtttgcg cctcagcttt tgatttgaat cttctacgct ttgtacgtat
    15121 atcttttcca gtcttgggat ctttccctaa ataagtttca aatccccaat atttttcgcc
    15181 attttgtaat ttataactaa aaaatgttgc catttgattc tctccttact tcattattgc
    15241 aggtagagaa gttatttgaa gaatcaatta tttttaaatt tatcgtgatt atcagttaaa
    15301 aattttgcga ttatactatt tttctcatat gggaaattgt attttttact aagatctgaa
    15361 ataaattttt ctgtttcaaa ttggttttta gtaattttat aaaagaaatc ctcgacagat
    15421 gaatactttg aaatatcttt ataggctaag tccatttcgt ctaatagctg ttttaaagaa
    15481 tcaatcattc tttctttttc accgtatttg aaatcaccat ttcttatctt gtcttgaagt
    15541 ttactgttgg ctcttttgaa aaatagctct ttagagatag agtgtctttg gttagcgtac
    15601 tcttcaatca gaggattgat attattagca agtatctcat ttagataaat ttcaaaatct
    15661 tttgagtaat attttgtaaa tatatttttt attataggat tcaataactc atagtcttta
    15721 tctagcgaaa acgtatcact atatctttct tgaatgtctt taaagacttt gtgaggtatt
    15781 tctgggaagt ccttaatata tagctcatac cctatatcga taaggtatga agttatatat
    15841 tcttctaaag ttccccattt aatccagtca attgttgaat tagtgatagt tgataataat
    15901 ttcagctttt gtggatttgg taaattggtc ccacgttccc agttggatat ggtcgcacgt
    15961 ggtgaattat ctattttcct tccaagttct tccatagtaa aaccacatga aaggcgtaat
    16021 tgtttgattc gttttcctgc ttctgaagga tttggcttca tcttttttct ccttttta
//
LOCUS       DAYYHB010000054        16059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000054 DAYYHB010000000
VERSION     DAYYHB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.055"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..158
                     /gene="rrf"
                     /locus_tag="ACHA6B_001767"
     rRNA            43..158
                     /gene="rrf"
                     /locus_tag="ACHA6B_001767"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(211..681)
                     /locus_tag="ACHA6B_001768"
     CDS             complement(211..681)
                     /locus_tag="ACHA6B_001768"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8949337.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(840..1298)
                     /locus_tag="ACHA6B_001769"
     CDS             complement(840..1298)
                     /locus_tag="ACHA6B_001769"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8949338.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1469..2614)
                     /locus_tag="ACHA6B_001770"
     CDS             complement(1469..2614)
                     /locus_tag="ACHA6B_001770"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8949339.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2627..3544)
                     /locus_tag="ACHA6B_001771"
     CDS             complement(2627..3544)
                     /locus_tag="ACHA6B_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8949340.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3972..4535
                     /gene="efp"
                     /locus_tag="ACHA6B_001772"
     CDS             3972..4535
                     /gene="efp"
                     /locus_tag="ACHA6B_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8949341.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4636..4806
                     /locus_tag="ACHA6B_001773"
     CDS             4636..4806
                     /locus_tag="ACHA6B_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949342.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            5021..6280
                     /gene="lysA"
                     /locus_tag="ACHA6B_001774"
     CDS             5021..6280
                     /gene="lysA"
                     /locus_tag="ACHA6B_001774"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8949343.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6267..7067
                     /gene="dapF"
                     /locus_tag="ACHA6B_001775"
     CDS             6267..7067
                     /gene="dapF"
                     /locus_tag="ACHA6B_001775"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8949344.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7070..7951
                     /gene="dapA"
                     /locus_tag="ACHA6B_001776"
     CDS             7070..7951
                     /gene="dapA"
                     /locus_tag="ACHA6B_001776"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8949345.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7948..8664
                     /gene="dapB"
                     /locus_tag="ACHA6B_001777"
     CDS             7948..8664
                     /gene="dapB"
                     /locus_tag="ACHA6B_001777"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8949346.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8670..9365
                     /gene="dapD"
                     /locus_tag="ACHA6B_001778"
     CDS             8670..9365
                     /gene="dapD"
                     /locus_tag="ACHA6B_001778"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8949347.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9381..10382
                     /locus_tag="ACHA6B_001779"
     CDS             9381..10382
                     /locus_tag="ACHA6B_001779"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8949348.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10379..11740
                     /locus_tag="ACHA6B_001780"
     CDS             10379..11740
                     /locus_tag="ACHA6B_001780"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8949349.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11700..12851
                     /locus_tag="ACHA6B_001781"
     CDS             11700..12851
                     /locus_tag="ACHA6B_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8949350.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12826..13995
                     /locus_tag="ACHA6B_001782"
     CDS             12826..13995
                     /locus_tag="ACHA6B_001782"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8949351.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14170..15549
                     /locus_tag="ACHA6B_001783"
     CDS             14170..15549
                     /locus_tag="ACHA6B_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8949352.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15830..16059
                     /locus_tag="ACHA6B_001784"
                     /pseudo
     CDS             15830..16059
                     /locus_tag="ACHA6B_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5251 a         2836 c         3426 g         4546 t
ORIGIN      
        1 tttgagtgaa caaaatttac tcaataaaag ataacaccac agtgtggtgg cgatagcgag
       61 aaggatacac ctgtaaccat gccgaacaca gaagttaagc ttcttagcgc cgattgtagt
      121 gaggggttgc cccttgtgag agtaggacgt cgccacgcaa aggcaaaaag atgaactaga
      181 aatagttcat ctttttttat ttttattaag ttaaagaacg gattcaattg cagcggtcat
      241 tttcaatgga gaagtttgtg gtgcaaatcg ttcgattact ttgccatttc tatcaatcaa
      301 aaatttagta aaattccatt ttattgcttt agtgacagta ccaggagctt catttttaag
      361 ataagtaaaa agaggatctg tattttctcc atttacgtca atcaattgat gcatagggaa
      421 ggtaacgcca taagtggttt tacaagcttc tgctgcttca gtcgctgaat ctagctcttg
      481 tttaaattga ttggaaggaa agcctaagat aaccagacct tgtgtctgat aagcttggta
      541 aagttcttct aattgtttaa attgaggtgc tagaccgcat tttgttgcag tgttaacaat
      601 gattatcggt ttgcctgcat attttttcaa tgaatagaca gtgccgtctt ctaaagtagt
      661 ttcaaaatta taaatagaca taaagttttc tcctttctat tttatataat aaaaagagcg
      721 ctaaactgaa aactagctat ttgttttccc gtttagaact cttttcagac aataaatatt
      781 taataaaata cgaagataag atagctatat tgcttctcat ctttacgttc ttttagaaac
      841 tatgcttcta cttcagacca ttcatcgcgt tttgctaaaa attcacgagc caaagcttgt
      901 gcaccttcta gtgtatggct ggcagcccag ccacattgtg tttcattgct tgcaggtact
      961 tcagtagctt caagaacatc tttcattgtt gcttcaagaa catctaaaat gtcttcgtag
     1021 ctttcatgat tcaatacagt caagtaaaag cctgtttggc atcccattgg agaccagtct
     1081 acgatgtaat ctgcgtgatt gcggattagt tcggcagtca agtgctcaag tgaatgtaga
     1141 cttggcatgt ccatgtgttc tttatttggt tgtttaaagc ggacgtcgta tttaatgatg
     1201 acatcgccat gcgcacccgt ttttcgatca gctacacgta cataaggtgc tttgactttt
     1261 gtatggtcaa gattaaaact ttcgacattc attctcattt gtaagactcc tatctgtttg
     1321 ttcattcgtt ttgatgcttt tcactaaaga attatttagt gaaatttaca aaaacgtctt
     1381 tctacttgat tctaacactt atcgtttcca aggtgtagca atctgaaata ttttttagat
     1441 ttttctgcta agtcgtttat tgggacttct agttcttgat tctgtcaaat gcctgagcta
     1501 aatcgtttat gagatcctga ccatactcaa ttcctacaga aagacggatc aagccgtcct
     1561 ttatgccagc ctcttctcgt ttttcttttg gaatagacgc atgagtcatg acagaaggaa
     1621 tttccactaa actttctacg ccacctaaac tttctgctaa agtaaatagt tggagtgatt
     1681 caacaaatgg aatagcagat tcttcatttt ttaaagtaaa agaaatcatt ccgctaaagc
     1741 cgctcatctg ctgcttagca actccatgga gttcatgatc gggtaatcct ggatagtaga
     1801 ccttttctac tgctggatga gaaaatagaa aatcagctac cacaaaagca tttttttgat
     1861 gttcctccat acgaacactt agtgtcttga ttccgcgctg aagtaaccag ctatcttggg
     1921 gaccaagtac ggctccgata gcattttggt aaaatccgat ctgatcggct agatctttat
     1981 ggttagaagt cactaaacca gctactacat cgctgtgtcc gcctagatat ttggtaccgc
     2041 tatggacaac gatatcagaa ccgagatcta aaggtctttg aaaataagga gtagcaaatg
     2101 tattgtcagc aataactatc aatccatgtt gatgagccag agtggcactt ttttctaaat
     2161 cagtaatctt tagaagcgga ttgctaggtg tttcaagata aagtgctttt gtattgggtt
     2221 tgatggattg ttctatttta tcaagattac tcgtatctat gatcgtgtac tcgagaccat
     2281 ttttagtaag aactttgtca aatagacgga atgttcctcc atagacatca tctcctaata
     2341 agatatgatc gccagcttga aaaagagaaa aaacggcatg aatcccagaa agaccagaag
     2401 aaaaagcgaa tcctcgaaca ccgccttcca aatcagcaat cagttcttcc aatgcaaaac
     2461 gagtaggatt accagatctt gaatattcat attgtttagg ctggccgacg ccatcttgcc
     2521 gataagtgga tgtttgataa ataggtacac tgacagctcc tgttgtggga tcttcactga
     2581 tgcctccgtg gatcaatttt gtttgaatgt gcatgtttct ttcctcctat aagtaaatgt
     2641 tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga tgaccaggtg
     2701 gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa cttccaacta
     2761 atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca tcagagattg
     2821 tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct acaccgatac
     2881 cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct ggttctacac
     2941 cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca gcaaaggttc
     3001 caccactccc aattcctgct acaaaactat ggatattttc tttgatttca tgaaagattt
     3061 ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat tgaagaggca
     3121 gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca ccgatgattc
     3181 cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat tgttgttttt
     3241 ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg agtgcggcca
     3301 gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt ttttcattga
     3361 tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct ttgatacttc
     3421 ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc atagaatagt
     3481 gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt gtcgagataa
     3541 tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa atcagctcct
     3601 tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta ctcattctat
     3661 gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat acaacagcaa
     3721 cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa aatttttatt
     3781 aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata tttcgatagc
     3841 atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa gatagagaat
     3901 actctagttt tctggccggt tgtctgatac aataaagttg attatgataa agaaaagaag
     3961 gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc gtacaagacg
     4021 gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga aatacagtta
     4081 tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg ttcagaccag
     4141 aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg tacacgatgg
     4201 atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt cctgtagaaa
     4261 cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc caatttttcg
     4321 gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt gtggaaacac
     4381 aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca atggaaactg
     4441 gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa atcaatacag
     4501 cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg ataagaattt
     4561 gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca agaggttttt
     4621 tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat tttgaaagat
     4681 gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat tgaaatgatt
     4741 cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat attttttcat
     4801 cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag ttcgggtatt
     4861 ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca
     4921 attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt
     4981 ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg
     5041 cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga
     5101 agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga
     5161 tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg
     5221 tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc
     5281 tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat
     5341 ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt
     5401 attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt
     5461 cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg
     5521 catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta
     5581 tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg
     5641 gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt
     5701 gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga
     5761 agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac
     5821 cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact
     5881 atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg
     5941 gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc
     6001 aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg
     6061 cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt
     6121 ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc
     6181 agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc
     6241 tgatctacta aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg
     6301 attttatctt attagattac gcagaagaca cagattatag tcaattagcc gcttctttat
     6361 gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa
     6421 tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt
     6481 ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat
     6541 tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata
     6601 tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa
     6661 accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga
     6721 ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac
     6781 tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca
     6841 atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg
     6901 gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc
     6961 aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa
     7021 tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg
     7081 ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt
     7141 ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag ttttaggtac
     7201 aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt
     7261 aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga
     7321 aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac
     7381 cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga
     7441 agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc
     7501 aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc
     7561 aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta
     7621 cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag
     7681 tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg
     7741 aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt
     7801 cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg
     7861 acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct
     7921 tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg
     7981 ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg
     8041 gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt
     8101 tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta
     8161 gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa
     8221 catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta
     8281 atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat
     8341 agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa
     8401 gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt
     8461 ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct
     8521 actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc
     8581 gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat
     8641 ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc
     8701 aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat
     8761 ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga
     8821 cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg
     8881 ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac
     8941 caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag
     9001 cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct
     9061 taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag
     9121 gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg
     9181 cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag
     9241 cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat
     9301 taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat
     9361 tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat
     9421 tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga
     9481 aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca
     9541 ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag
     9601 gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag
     9661 ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc
     9721 tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg
     9781 tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct
     9841 atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg
     9901 acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc
     9961 agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga
    10021 ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg
    10081 gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag
    10141 agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc
    10201 cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca
    10261 cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg
    10321 ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat
    10381 gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt
    10441 caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc
    10501 tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc
    10561 ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat
    10621 tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca
    10681 gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct
    10741 gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat
    10801 tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc
    10861 atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa
    10921 agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt
    10981 gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg
    11041 aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc
    11101 ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat
    11161 ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca
    11221 cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat
    11281 cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat
    11341 ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt
    11401 gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga
    11461 aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg
    11521 gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt
    11581 agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt
    11641 accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga
    11701 tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca
    11761 agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg
    11821 gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa
    11881 cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg
    11941 aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg
    12001 actttataac taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat
    12061 cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg
    12121 aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct
    12181 ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt
    12241 ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt
    12301 tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat
    12361 ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg
    12421 gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt
    12481 tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag
    12541 ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat
    12601 tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg
    12661 tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg
    12721 atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc
    12781 ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc
    12841 aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag
    12901 caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact
    12961 cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc
    13021 ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat
    13081 ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt
    13141 gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct
    13201 tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact
    13261 tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga
    13321 tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct
    13381 gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt
    13441 gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt
    13501 ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga
    13561 gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat
    13621 ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg
    13681 gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag
    13741 caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc
    13801 aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag
    13861 gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg
    13921 atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt
    13981 ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat
    14041 tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca
    14101 taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag
    14161 gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa
    14221 tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca
    14281 ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca
    14341 gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt
    14401 ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc
    14461 gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc
    14521 aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga
    14581 tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct
    14641 gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa
    14701 gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg
    14761 acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa
    14821 atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc
    14881 ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg
    14941 atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt
    15001 ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa
    15061 atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt
    15121 tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc
    15181 tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta
    15241 gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa
    15301 ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga
    15361 ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc
    15421 caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt
    15481 gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt
    15541 gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt
    15601 aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc
    15661 gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg
    15721 taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg
    15781 ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg
    15841 attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa
    15901 gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat
    15961 caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac
    16021 gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYHB010000055        15933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000055 DAYYHB010000000
VERSION     DAYYHB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.056"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..3661
                     /locus_tag="ACHA6B_001785"
     CDS             134..3661
                     /locus_tag="ACHA6B_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8949353.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3658..7380
                     /gene="addA"
                     /locus_tag="ACHA6B_001786"
     CDS             3658..7380
                     /gene="addA"
                     /locus_tag="ACHA6B_001786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8949354.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILTKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7449..7790
                     /locus_tag="ACHA6B_001787"
     CDS             7449..7790
                     /locus_tag="ACHA6B_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8949355.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8099..9142
                     /gene="pheS"
                     /locus_tag="ACHA6B_001788"
     CDS             8099..9142
                     /gene="pheS"
                     /locus_tag="ACHA6B_001788"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8949356.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9147..11567
                     /gene="pheT"
                     /locus_tag="ACHA6B_001789"
     CDS             9147..11567
                     /gene="pheT"
                     /locus_tag="ACHA6B_001789"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8949357.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACHA6B_001790"
     CDS             complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="ACHA6B_001790"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8949358.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13001..13738
                     /locus_tag="ACHA6B_001791"
     CDS             13001..13738
                     /locus_tag="ACHA6B_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8949359.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13752..14567
                     /locus_tag="ACHA6B_001792"
     CDS             13752..14567
                     /locus_tag="ACHA6B_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8949360.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14580..15227
                     /locus_tag="ACHA6B_001793"
     CDS             14580..15227
                     /locus_tag="ACHA6B_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8949361.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15243..15899
                     /locus_tag="ACHA6B_001794"
     CDS             15243..15899
                     /locus_tag="ACHA6B_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8949362.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5342 a         2746 c         3376 g         4469 t
ORIGIN      
        1 ttttttgcgt atacttaaaa aagtagaaat atcagaacct taaaaggata taatttgaag
       61 aatatagaac aaatgttcag tattttttga tataataaag gagcaccgta tcatgtgttg
      121 aaggaggaaa agcatgtctt tacaatttat tcttggaaac gggaaaaaag atcaccgtca
      181 agtcttgatc gacgaagcat ccgcttggct cgaccaggat gcaagtaacc aagtattttt
      241 ccttgttccc aactacagca aattcgaaca ggaacaagaa attttagcgg agatgcgaag
      301 aagaagcgga aaaaaaactt tcagcacaac aaacgttcaa gtatttagtt tttatcgtct
      361 ggcttggtat ttgctgcaac agacaactct tcttacagga aatgaattat cagaatcagg
      421 atctgcaatg atcttgcgac agattttaga aaaaaatgag gaagaattaa caatctttcg
      481 cggagaaatc aataaaagag ggtttattcg tcaattacaa gaactctaca gtgaattgcg
      541 tacaggaaat attcaaccag aagacctgat gcttttattt ggtgattcac ctaatgcaga
      601 agaccagcaa cttaaaatga aagacttgaa gctgattttt tcagcttatg acagcgaatt
      661 gactcaacgt gcactaacaa atgaagatgc actatctata ctagcaaatt atatgcagac
      721 acaagatttc aacaacgtca aatttattgt cagtggtttt tctcgaatca atggtcaaga
      781 gtatcaatta ctgcaggcaa tgatggaaaa ggggcaattg gttgtcgatt tattgctgga
      841 tcgtccttat acagcggatc ttccagaagt acttacgctc tttcatgaga ctggaaagct
      901 ttatttccgt ctttttcaaa gtgcaagaga acggaaagtc cctatattag tggaccgatt
      961 tgcaccagag cagactcgtc cgacagagct tcaactgctg caaacaatgt gggaagagac
     1021 atctagccaa aagaaagcgg cagtacacaa aataggggga caagagcatc tccatttgtg
     1081 ggaagctgaa aatccaactg aagaagtccg acaattagct gttgaaatca gacgactagt
     1141 cagtgaagaa aactaccgtt atcgtgatat ccaagtgctg acaaaaaata tgtctttata
     1201 tggaaatatc cttcggccga tctttcaaga aatggcaatt cctttttata tcgatgaaga
     1261 acaaatgatg gaaaatcatc cgctgatcga atggatcaac agtctgttcg ctcttgaccg
     1321 ctattcttat cggctaagtg atattatgcg ttttttcaaa actgaattat ttctgtcgac
     1381 ggaagcagaa accgaacttg atgaatggaa acaagagcga gatgaattca ggaaaaaact
     1441 ggacatcact gaaaatgtag cacttgctta caattatcaa ggaacttatt ggactcgtga
     1501 aaaagactgg cagtttgtgg cgtatgattt tgaagcagaa cagattgaag acgtacagta
     1561 tttggaagaa cagtcaaata cgattagacg attatttcag aaagctgtac cagtattttt
     1621 gaaaaaaata aagaaaagca aaacaggaca agaggccgca aatgtttttt atcaattttt
     1681 gatccaaagt ggcgtacagc aacaattatt gttttggcgt aatcaggaag tagacagagg
     1741 aaacttagaa gcggcaagaa accatgagca gacatgggga gcgctgattg atctgttgga
     1801 tgaatatgta gtaatttatg gtgaatcggc ttttgactgg tcactgttcc aagaaatcat
     1861 tacaagtggt ctagaaaatc taagttatgg taaaatccct accgcaatcg accaagtgcg
     1921 aatcaatcgt ttggaattag ttcgaccaaa tcaagctgcc gtcacttttg cattagggtt
     1981 gaatgatcaa gtgtttccag accgaaaaga gacaaaagga ttgttgtcca gtgaagaacg
     2041 agaagtatta aatcaggcct tgccagatgg acagttttta ttcgacccat caaaggaaag
     2101 tatctctttt gagcctttcc aagcctatct tgtttttctt tccggaacag atcgattata
     2161 tcttagttat gcacaaagct atgacacaga tggttcgctg aagatgtctc cttatttgcg
     2221 tagaatcaca gaatatttat ctgttcccgt cgagagaaaa gagcatttga cgatcgaaag
     2281 cgaccctaat tgttatgtag ggacttacag aagcggaatc aatacagtca ataggattta
     2341 tcgattagct ttagatgaaa aaaaacctgt accaaagcac tggcaaaagc tcaaagaatt
     2401 agtactgaat tcgtcatggc gaaattttgc tttacagatt tttgaaagtc aggaacattt
     2461 aaatgttcct gtttcgatgc cagaaaaaat ggcggaagag ctttatggaa aagagattta
     2521 tacttcaatt tcacgtatgg agaattttta taattgtgaa tacaaatact tcatgcaatt
     2581 tggtttgaaa ttgaaagaac gaactgttta tggtctaact ccagcagcaa ctggtgattt
     2641 ttatcatgaa tcattggatc gtttcttcaa gctactgttt tcaaaccaat tatccttagt
     2701 gaatatgtcc gatgcgcagc gtagagactt tactgaaaaa gtgctgcagg aagtattcgg
     2761 agaacttcgg tttgagatat tggatagttc tgcaagaatg aactatatcc gctaccagtt
     2821 aggacagacg attcaaaaag tgacatgggc tttgcaaaaa cagagcaaaa atacaggcat
     2881 gcatcctctt cagacagaga ttctatttgg tcagattgct ggagcaaaag gtatcacagg
     2941 tctggaatta ccgttgagta atggtggaaa actccatgtt cgagggaaaa tcgatcgaat
     3001 cgatgtagct tctgaacaag aagatacttg gctaagcgtc gttgattaca aatctagcgg
     3061 acgttcattc gatgtcacag aagcttatta tggcatggct atgcaattat tgacgtatct
     3121 ggacgtagca ttgatggatg cggtacgttt gactggaaag gcggctgtta aaccagcagg
     3181 atcatattat ttacatgttc ataatcctgt attgacggag acagatgatg tagagaagaa
     3241 tacattgaaa aaatttagtt atgatggcgt ttttgtcgat gatcccgctc ttttagaagt
     3301 actagatcat tcactggaag caaaagaaca atcactgatc tatccagtga aaaaaaatgc
     3361 gaaagatgaa taccaaaaag gtagtttcag taaagacaaa tttttcacgg aagaagaact
     3421 gcaactattc atggcgcata atcgggaaaa tatgcggtta gctggagatg cgattacttc
     3481 cggagaaatc aaactaaatc cagcttataa agataaacag cggattgcct gcgaattttg
     3541 tccattcaga agcatctgta cgtttgatgt gatgctgaaa gaaaataatt accaccgtct
     3601 ggagaaaata gataaggaag aagcactgcg tagaatattg aaaaggagtg aagaagagtg
     3661 aatcaaattc ctttaaaacc gcaaaatgaa cgattcacag atgatcagtg gcaagcgatt
     3721 tttgatcaag gagataatct attagtctct gcgtctgccg gatcaggtaa aactacagtg
     3781 cttgttcgaa gagtgattga aaaactaaaa atgggttttg atatcgatga attattgatt
     3841 gtgacattta ctgaagctgc cgcaagagaa atgaaagaaa gaattcaaga ggcattacaa
     3901 gaatccgtca atagcgaaag tgaccctgta cgtagacagc attttactaa gcagttagtt
     3961 ttattgccca cagcgaatat ctcaacctta catgcttttt gcctgacagt tattcgacgt
     4021 tattattact taattgatat tgatccagtc tttcgcatgt taacagatga aacagaaacg
     4081 atcttgacga aggaggatgt gtgggacgag ctgagagaag cactttatgc agaaaatgat
     4141 gaacgatttt tccaattaac aatgaatttt tctaatgatc gttcagatga cggcttgacg
     4201 aatctggtgt tttctcttta tgagtttgcc cgtgccaacc ctgaccccca aaaatggcta
     4261 gaacagttaa gcgataatta tcgtttgcct gaggggctag cgaaatctcg attatatcaa
     4321 aaacaaatcc gtcctttagt tttggcagat atataccaat gtgtacagtt atatgaacag
     4381 atgactcagt tagcacaagg tgaaggatta gaaaaaatgc atgagcaagt agctggagaa
     4441 caacagcaga tcaagaatat ctatgaagcg ttctcgcaag atcggttaga agaagcttat
     4501 gctggtttgg aacaactaac tttttcaact tttaagagca gtcggaaagc agaattgaaa
     4561 gaaatttcca atgaagtcaa gggcatgagg gataaagcaa aaaaactgat tcagcagata
     4621 agtaaaagtt attttcctgt ttccccaagc caaatggaag aattaacgga caaagcacta
     4681 ccgttagtcg aagaaatgac aaaagtcacg caaagcttca tggacggttt cagtatgcgt
     4741 aaacgggaaa aaggtgtgct ggatttcaat gatctggaac atttagcgtt gcagattctc
     4801 acagaaaaaa cgaaagatgt atggctgcca agtgaagctt caaaacatta tcgaaaaaaa
     4861 ttcaaagaag taatggtaga tgaatatcaa gacgtgaacc aattacaaga agccatttta
     4921 tactggctaa gagaacccga tgatacaaaa ggaaatatgt tcatggttgg ggatgtcaaa
     4981 caatccatct attctttccg tttagcagat cctagtttat ttatcgggaa atacgagaat
     5041 ttctcaaaga aagaaggcgg acgtcgaatc gttttggctg agaacttccg ctctagaaaa
     5101 gaagtccttt cttttacgaa tcttattttt gaacaactaa tggatccagc agtgggtcaa
     5161 atcaattatg acgaagcagc caaactgatc caaggatttt cagactttcc agagaatgaa
     5221 cagtttgagc cggaaatcat gatctatgaa aaagaacagg aagaatcaga gattgagatt
     5281 ccgacggatg atatactgga agacaaaaca gaaggcgaac tttttatgac aggactaaaa
     5341 attcgtcaat taattgattc ctcctttatg atttatgata aaaaatcaaa aaaaagtcgt
     5401 ccgatagagt acaaagacat tgttctcttg acacctacaa agaagaacaa cttaacgatt
     5461 ttagagattt tcaaaacatt ggatattcca ttagagatga atgatgctca aaactatttt
     5521 caagcgacag aaattcgcac gatgatctcg ctgctacaat taatcgataa tccctatcaa
     5581 gatattccgc ttgcggctgt attgcgctca ccgatcgtgg gtctgattga accagaatta
     5641 gccagcattc gattagctaa ccgtgcacat acttactatg atgctgtgct tgcttatcaa
     5701 gcatcaaacg aagatgaatt agcagctaag ctggaacatt ttggcaagca actagagcat
     5761 tggcgagaac ttgctcgccg ttcttcgatc actgacttgt tatgggatat ctattatgag
     5821 acgggatatc tagagtatgt ggtcggtctg ccagcagggg cacagagaca agccaatctt
     5881 tatgcattgg ttgatcgtgc caaagcttac gagcaaagtt ctttcagagg attataccag
     5941 ttcgtgcgtt tcattgaaaa aatgcaagaa aaagataaag acttagcaga accagtcatt
     6001 tcaatagaag ataatgctgt tcgcgttatg acgatccatg caagtaaagg cttggaattc
     6061 ccagttgtat ttttgttaga tatgacaaag gagttcaact tacaagactt gcgcaatcgg
     6121 tatgcttttg aagaaaaact tggagcagga attcgctata tggatcctga aacacgagtg
     6181 ttatatgata cgttaccgtt tcaagccatc aaattggcaa agcaaaacaa attgttgtcg
     6241 gaagaaatgc gtaaattgta tgtaggattg actcgggcag aacaaaagct tttcattgtc
     6301 ggttcctata aaaataaaga gcaaatgatc caaacgtggt ctgaagcagc tgatcatgag
     6361 gagttagttt ttgatcctgc gctacgtttg aagggcagaa gcagcttgat gaattggatt
     6421 ggttacggat tgatccgtca tcctgaaatg cagaaatact tagaggaaga aatttcgact
     6481 tctcttcttc aacattcaaa tgcacagttt tctatttcat ggatgaatca gcaatcgatc
     6541 attgaacaaa gacagttgct tgctgaaaaa gaactggtta acttagatca gcagatgaaa
     6601 gaagatgaaa ctttattagc agattcgtta caaaaacgtc tggcatatga atatccttat
     6661 caagcttctt cacaaaccac aagttatcaa tctgtatctg aaatcaaacg attattcgaa
     6721 gatccagatg atacacaaga atcacgtttg actctggaaa gcagtcagaa caaagcagca
     6781 tcccgtcaat ttcgttatac gcaagaacaa ctagcagaac caaaattttt gcaaaaagat
     6841 cggcaagtgt cagcagctgc tgttggaact gctacgcatg ctttgttaca attactgcca
     6901 ttagagatgc caactacaga gagtatccat caaaaattac aggaattggt aaggaaacgg
     6961 ttagtagacg aaaaagtcgc gaaaaaagta gatgtttctt ctattatatg gttctttcaa
     7021 acagaacttg gtcaacagct gattgccaac aaagaaaacg taaaacgaga acaacctttt
     7081 tccatgctgc ttcctgctga cgaagtattt caagactatc caaatcaaga agatgaacta
     7141 ttaatccacg gaatcgtcga tggataccta gaagaaaaag atcatctcaa tatctatgat
     7201 tttaaaacag attttatttt acatccggat gatccagcag aaatagatgc aatcgtccaa
     7261 aaatatcaag gacagctaag actttaccaa caagcaatgt cagaagcttt gaataagcca
     7321 gtagaaaatg ttttcctaat tttgcttcga gtgaaacaaa ttataaacat taacaaataa
     7381 ttataattga gcaaacttga cttatgtttt gtaaggatta tgctatactc tgggaagagg
     7441 tgagtatgat ggagctaaca cagacagaat tcgttttatg ccctaaattt gaaaaaacat
     7501 tctctatttt aggaaaaaaa tggaacggat tgattatcga tgtactactc gaaaacggtc
     7561 cgcaacgctt tgttgagctt gcggcaaaaa tcccgatggt cagtgatcga gtattagtcg
     7621 aacgcttgaa ggaattagaa aaagaaggaa tagtcactcg aaactttgcc tgtagcgaaa
     7681 agagccgttc agaatatctc ttgactgaaa aaggagcaga cttgcaaaaa gcgatgcgtc
     7741 aaatacaagt ttggagcgaa aaatggatca cagaggaaga atgcggttga ttttgtaaaa
     7801 aaaatcttgt aaactatagt ccatacgaat aagtaaagaa cgatgacgaa aaagagtagt
     7861 ttggctaaac cagttcaggg agatctgtcg cagactgaaa gcagatcctg tgtttgaaaa
     7921 atgaagttca ctttccgagt tcgtctgata aaatcagacc ggcaattgtc gttaacgaag
     7981 taagagtgcg gtgtaagtca tttatccgca aattaggatg gtaacgcgaa gacctcgttc
     8041 cttaacagga atgaggtctt tttgttttgt atttggaatt taaaggagga acaagcacat
     8101 gtctctacaa gcacaattag agagcttgaa agaagcaacg ctcgtcaaaa tcacagcggc
     8161 acaagattta aatgaattaa atcaaatacg tgttgaaacg ttaggaaaaa aaggaccgat
     8221 cacagaagta ttacgtggga tgaaagatct aagtccagaa gagcgaccaa aagtcggaag
     8281 tttcgcaaat gagatccgag atcttttgac agaagagatc gagcgaaaaa aacaagtatt
     8341 agaagaagaa gcgttaaatg cagctttggc aaaagaaaca atagacgtga cactacctgg
     8401 tagaaaagta aaacatggta cacgtcatat cgtgacacaa atcatggaag aaattgaaga
     8461 tatttttgtg ggtatgggct atcagatcgt agaaggatat gaagtagaat ctgatcatta
     8521 caatttcgaa cggatgaatc tgccaaaaga tcatccagca agggacatgc aagatacttt
     8581 ctatatttca gacgagatat tgattcggac acatacttca ccagtccaag cacggacaat
     8641 ggaaaaacat gatttctcta agggtgctct acgaatgatc tcgccaggga aagtcttccg
     8701 cagagataca gatgatgcga cccacagtca tcagttccat caaattgaag ggctagttgt
     8761 tgataaaaac atcacaatgg gcgaccttaa aggaacatta gaagtcgtaa tgaaaaaaat
     8821 gtttggggaa gatcgtgaaa ttcgtttgcg tccaagttat ttcccattta cggaaccatc
     8881 agtagaagta gatgtcagct gtttcaaatg tggtggtgcc ggttgtaacg tatgtaaata
     8941 cactggatgg atcgaaattt taggagctgg catggtacac ccgaatgtgt tgaagatgtc
     9001 aggaatcgat ccagaagaat attcaggctt tgctttcggc cttggaccag accgcgtagc
     9061 tatgttgcgg tatggagtga atgatatccg taatttctat caaaatgact tacgtttctt
     9121 aagtcagttc aagggggagt aagaaaatgt tagtttcata taaatggctc aatcgttatg
     9181 tagatttatc gaatgtaaca ccaaaagaat tagcggataa gatgtctgtg acaggaatcg
     9241 aagtagaggg agtaacggtt ccagaagaag gcttgaaaaa aatcgttgtc ggtgaagtca
     9301 aggaatgcgt accacatcca gattctgatc acctttctat ttgtcaagtc gatattggcg
     9361 aagacgaact gtctcaaatc gtttgtggag cgcctaatgt gaaggttgga atcaaagtga
     9421 ttgttgcact tcctaactca cgtatcgctg gtaatgtaaa aatcaaaaaa ggaaaaatgc
     9481 gtggccaagt atcaaatggc atgatttgct cattacaaga aattggctat tcagatagtg
     9541 ttataccgaa agaatacgct gaaggtatct attatatgcc acaagaagct gtaaatggcg
     9601 atccagtttt tccttatttg gatatggatg attcgattat tgaattatct atcacaccga
     9661 atcgtgcgga tgcattgtca atgcgtggcg tagcttatga agttggggca atttatcgtc
     9721 agactcctac attcgatgac gaaaagctgg tagaatcctc acgtcctgct agcgataaaa
     9781 ttcaagtgga agttgaagat aaagaagcag ttcctgctta tcaaatcaga atcattgaag
     9841 gagtaagaat ccagccaagt cctcaatggc tgcaaaatct tttgatgaac gaaggcattc
     9901 gtccaatcaa caatgtagta gatgtgacga actatatttt gcttttattc ggacaaccac
     9961 tacatgcatt tgactacgac aaattgaaat caaaagagat ctttgtccgc cgtgcaaaag
    10021 aaaatgaaac attagtaaca ctagatggag aatctcggga attgacaact gacaatctcg
    10081 tcattactaa cggcaaagaa ccagttgctt tagctggtgt catgggaggg ttagactcag
    10141 agatcacgaa tgaaacaaca acagttgcac tagaatctgc tttatttgat ccaatttcga
    10201 ttcgtcgtac agcaaaacaa tttaatttgc gtagtgaatc ctctgcacgt tttgaaaaag
    10261 gaatcaacca tgcgactgtt ggagaagcca gtgaggttgc tgcagcaatg atcgtttctc
    10321 ttgcgggtgg ggaagttcta caaggcgcgg tcaaaggctc agagatcaaa gcagaagacg
    10381 tatcagtgaa gatttcactt gaacgcatca atcaatattt aggaacatct cttgtcgtta
    10441 aagaagtaaa tgagattttt gaagcacttg gatttagcta tgaagaaaag gagtatgttt
    10501 attccgtagt agtaccacca agacgctggg atatccaaat cgaagcagat attatcgaag
    10561 aagttgctcg tatttatgga tacgaccagt tgccatctac acttccaagt ggagaaacgg
    10621 ttgctggaag cttgtcaaaa gaacaagaaa ctactcgtaa aattcgtaca atctttgaag
    10681 gaagcggatt gagcgaagca atcagttatg cattgacaac agaagaaaaa tcaagacagt
    10741 ttgttataaa tgaaagtaat attacacgtt tggactggcc aatgagcgaa gaacgttctg
    10801 tattacgaat gaatttgatc agcggactat tagatgatat cagttataac actgcccgaa
    10861 aaaatacaga gattgcttta tatgaaattg gtcgggtgtt cttccaagaa aatgatccac
    10921 gtacacatct gccgcaagaa gtaaaacatg cagcaatcgc tatttctggt atttgggaag
    10981 aaaaagattg gcagacgaaa gctcaaaatg tagacttttt caccatcaag ggtcttttag
    11041 aaaacttatt tgaacaacta ggcatcactg aagatgtcca ttatcaaaca gtgacagaaa
    11101 tgaaggaaat gcatcctgga agaactgctg cgatttacct tggcgaaaaa tttattggtt
    11161 ttgtcggtca agtgcatcca acgattacaa aaagttatga tattccagaa acatatgttg
    11221 ctgagtttga tttatctgct atagtggaag ctgctcaaaa agggatcgtt ttcgaagcag
    11281 tgacaaaatt cccagcagtc agccgggaca tcgctttgct tgtaaaagaa actgtgacaa
    11341 atcaggaatt aacagaagtt atccgttctg ctgccggaag atttttaaca gatatccaat
    11401 tatttgatgt ctatcaagga gaaaacattg aaaaaggaca taaatccatg gcgtatagcc
    11461 tgacattcgt caacccagaa gcaacattga cagatgaaga aatcaataaa gcaatggaaa
    11521 aagtcgaaaa agcgttggta gaaaacctag aggcaagtat tcgctaagaa ggaaaatata
    11581 gtaagtaacg atcaagtagc ttttgaagac catacatttg aaaaaaagat gtgacacaac
    11641 ttcgtcacat ctcttttttt gattttaaaa aaggaatagc acagagacga gataaaaaaa
    11701 caggccttta cagcctgctt ttttagagta aatgaattaa tggaagtaat tgattcccat
    11761 tgctgatttt acttcagaaa gtgtttgtgc agctacttta gcagcttctt cactaccttt
    11821 acgtaagatt tccataacag ctgaaggatc tttttccaat tcttctcggc gcgcacggat
    11881 cggtgcaaat tcagcttcca atacatcaat gaggtatcgt ttgatcttta catcaccaag
    11941 tccgccgcgg cggtaatgag ctttcatttc ttcaattgct tctttatctg ttccgaatac
    12001 atccaagtag gtgaagacca tattcccttc tacttgtcca ggatcttgta catggatgtg
    12061 gtttggatca gtatacatac tcatgacttt tttctgtaag acatcggcag gatcggagat
    12121 ataaataccg tttcctaacg atttactcat tttgccattt ccatcgattc caggtaatct
    12181 acccattcct tttggtggga atacgccttt tggttccacc aagacttcac catatgtatg
    12241 attgaagctt tgtacgattt cctgtgtctg ctctagcatt ggtttttgat cctcaccaac
    12301 aggtacaaga ttcgctttga atgcagtgat atctgctgct tgagaaacag gatagataaa
    12361 gaatcctgta ggcacgcttt caccgaattt tttttgttcg atttctgttt tgacagtcgg
    12421 attgcgccga acgcgtccta cgctgactaa gttcaaataa tacatggtta attcagctaa
    12481 ttccggaatt tgtgattgga taaataaagt agattttgca ggatctaatc ccacggctaa
    12541 atagtctaaa gcaacttgca agacatttga tgaaaccttc tcaggatttt tagcattatc
    12601 tgtcagtgct tgcatgtcag caatcatgac aaacagttgg ttgtcttcat ctgcctgcat
    12661 agccactcgg gtttttagag aaccgacgta atgccctaaa tgcagcttcc cagttggtcg
    12721 atctcccgtt aaaataatat ttttcaaaaa agacacctct ttacgatttg taagtctgag
    12781 cacttaatat cttaccgttt ttcctgaaaa gaaacaacag ttcctaagga aaaaacaagg
    12841 aaagaggata aaataactaa tagattagaa atggaagaaa tatttatggg agaataaacg
    12901 ttctaagaaa ctttacaaat tactaaacta tcagatataa tgtattttat tatacatctt
    12961 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
    13021 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
    13081 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
    13141 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
    13201 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
    13261 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
    13321 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
    13381 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
    13441 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
    13501 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
    13561 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
    13621 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
    13681 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
    13741 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
    13801 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
    13861 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
    13921 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
    13981 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
    14041 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
    14101 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
    14161 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
    14221 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
    14281 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
    14341 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
    14401 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
    14461 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
    14521 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
    14581 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
    14641 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
    14701 aattatcaaa gaataaagta gtgagcacct tagcaaaggc atatgtagaa ttttttagaa
    14761 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
    14821 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
    14881 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
    14941 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
    15001 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
    15061 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
    15121 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
    15181 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
    15241 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
    15301 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
    15361 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
    15421 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
    15481 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
    15541 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
    15601 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
    15661 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
    15721 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
    15781 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
    15841 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
    15901 agatgattct tttagcggtt gtggcaaact aaa
//
LOCUS       DAYYHB010000056        15754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000056 DAYYHB010000000
VERSION     DAYYHB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.057"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACHA6B_001795"
     CDS             14..1141
                     /locus_tag="ACHA6B_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949363.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACHA6B_001796"
     CDS             complement(1359..2501)
                     /locus_tag="ACHA6B_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949364.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACHA6B_001797"
     CDS             complement(2506..2691)
                     /locus_tag="ACHA6B_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8949365.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACHA6B_001798"
     CDS             complement(3045..3290)
                     /locus_tag="ACHA6B_001798"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949366.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACHA6B_001799"
     CDS             complement(3284..3685)
                     /locus_tag="ACHA6B_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8949367.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACHA6B_001800"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACHA6B_001800"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8949368.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACHA6B_001801"
     CDS             complement(5703..7469)
                     /locus_tag="ACHA6B_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8949369.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACHA6B_001802"
     CDS             complement(7482..8276)
                     /locus_tag="ACHA6B_001802"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8949370.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="ACHA6B_001803"
     CDS             complement(8286..9662)
                     /locus_tag="ACHA6B_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8949371.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFPYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /locus_tag="ACHA6B_001804"
     CDS             complement(9782..10429)
                     /locus_tag="ACHA6B_001804"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8949372.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACHA6B_001805"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="ACHA6B_001805"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8949373.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACHA6B_001806"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="ACHA6B_001806"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8949374.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="ACHA6B_001807"
     CDS             complement(13096..14727)
                     /locus_tag="ACHA6B_001807"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8949375.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..15702
                     /locus_tag="ACHA6B_001808"
     CDS             15031..15702
                     /locus_tag="ACHA6B_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8949376.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         5104 a         3072 c         2130 g         5448 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aaggggtact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataag ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgagcaaaat
    15661 attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac tctttttaaa
    15721 ttaaatggtt gttctgatca gaaaaatatc gctt
//
LOCUS       DAYYHB010000057        15436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000057 DAYYHB010000000
VERSION     DAYYHB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.058"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..2165
                     /locus_tag="ACHA6B_001809"
     CDS             105..2165
                     /locus_tag="ACHA6B_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8949377.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2248..2817
                     /locus_tag="ACHA6B_001810"
     CDS             2248..2817
                     /locus_tag="ACHA6B_001810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949378.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2992..3798)
                     /locus_tag="ACHA6B_001811"
     CDS             complement(2992..3798)
                     /locus_tag="ACHA6B_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949379.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3936..5450
                     /locus_tag="ACHA6B_001812"
     CDS             3936..5450
                     /locus_tag="ACHA6B_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8949380.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5853..6836)
                     /locus_tag="ACHA6B_001813"
     CDS             complement(5853..6836)
                     /locus_tag="ACHA6B_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8949381.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6841..7488)
                     /locus_tag="ACHA6B_001814"
     CDS             complement(6841..7488)
                     /locus_tag="ACHA6B_001814"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8949382.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7573..8205)
                     /locus_tag="ACHA6B_001815"
     CDS             complement(7573..8205)
                     /locus_tag="ACHA6B_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8949383.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8221..8772)
                     /gene="coaC"
                     /locus_tag="ACHA6B_001816"
     CDS             complement(8221..8772)
                     /gene="coaC"
                     /locus_tag="ACHA6B_001816"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8949384.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8769..9506)
                     /locus_tag="ACHA6B_001817"
     CDS             complement(8769..9506)
                     /locus_tag="ACHA6B_001817"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8949385.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9503..10492)
                     /locus_tag="ACHA6B_001818"
     CDS             complement(9503..10492)
                     /locus_tag="ACHA6B_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8949386.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10750..11292
                     /locus_tag="ACHA6B_001819"
     CDS             10750..11292
                     /locus_tag="ACHA6B_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8949387.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11309..12394
                     /locus_tag="ACHA6B_001820"
     CDS             11309..12394
                     /locus_tag="ACHA6B_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949388.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12406..13212
                     /locus_tag="ACHA6B_001821"
     CDS             12406..13212
                     /locus_tag="ACHA6B_001821"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949389.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13213..14040
                     /locus_tag="ACHA6B_001822"
     CDS             13213..14040
                     /locus_tag="ACHA6B_001822"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949390.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14037..15110
                     /locus_tag="ACHA6B_001823"
     CDS             14037..15110
                     /locus_tag="ACHA6B_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8949391.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4958 a         2789 c         3142 g         4547 t
ORIGIN      
        1 ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa
       61 ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac
      121 gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa
      181 aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata
      241 tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg
      301 agaccactga ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc
      361 tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata aaccactact
      421 ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta
      481 tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt
      541 tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg
      601 aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt
      661 cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca
      721 aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt
      781 ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg
      841 ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt
      901 acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag
      961 aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat
     1021 taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag
     1081 aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag
     1141 aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg
     1201 ctgaattttc tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag
     1261 tcacttctat ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg
     1321 agatccttga agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa
     1381 ttacctttac ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg
     1441 ttcatccaaa cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag
     1501 gtaaacccgt accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg
     1561 gttatttgac caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt
     1621 cactccaaga actccatgct cagtcactat tacatgtttt accattaaac aaacagatag
     1681 atcaaaaaga acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa caaatggctc
     1741 atgcagtttc tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat
     1801 ctagaaaaat ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag
     1861 tttctggatt ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat
     1921 ttgttgctaa acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa
     1981 aacgcttgtc aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta
     2041 gtccgggaac acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc
     2101 gaggtatggc aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt
     2161 tctgatttat atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc
     2221 ataatggaga cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc
     2281 tggatgacaa gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa
     2341 tttcgatttt atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg
     2401 gaatttcaaa cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct
     2461 gaagaacttg aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc
     2521 tgtaccgctt ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca
     2581 gtgaaatcag attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa
     2641 gaatttccta ccctccatac ggacggacca atctatcttc acacacagac atggagccat
     2701 caggcgattg ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc
     2761 acacctaatc cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga
     2821 aatggtgaaa taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt
     2881 acttttttga gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg
     2941 ggcagtgaca aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt
     3001 gattttttta ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata
     3061 gaggtaacca aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat
     3121 agtatccatg attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc
     3181 atctgcattt tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt
     3241 tccgtcttta cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc
     3301 gaattgcagt aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat
     3361 cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt
     3421 tttattgagc aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc
     3481 atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac
     3541 tgttgaacta aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt
     3601 taatatcttt tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat
     3661 tgtatttccc cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg
     3721 taataaaata tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa
     3781 ggttttcaaa tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc
     3841 gtgtgtttac caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc
     3901 acttaatata gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat
     3961 taacagccgt tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc
     4021 agacaagttc tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat
     4081 atacagatcc aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg
     4141 cagggatggc agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa
     4201 agatgcctgt tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa
     4261 caaaatttca aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc
     4321 taaaaggtgg tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg
     4381 ctgatgcggt cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg
     4441 gagggatgag tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact
     4501 tagtggaggg gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag
     4561 atgtgacaga cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag
     4621 gagaagaacc aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg
     4681 ccagcaaaga atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc
     4741 aagaaggatc aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg
     4801 atatggataa aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg
     4861 aggttgttcg cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg
     4921 aaatggatac aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt
     4981 atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa
     5041 aaggatatgt cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa
     5101 aagaaacact agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg
     5161 ctcaattcaa ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga
     5221 tcaaaatcgc accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg
     5281 aagtattaga taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag
     5341 gtggcttaca tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg
     5401 ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt
     5461 aaataaaaag aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa
     5521 cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     5581 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
     5641 tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     5701 gctctaaaca cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt
     5761 cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt
     5821 ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt
     5881 ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat
     5941 gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata
     6001 aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc
     6061 agttggacac gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct
     6121 agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag
     6181 taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt
     6241 acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta
     6301 caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt
     6361 ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt
     6421 tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct
     6481 gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca
     6541 ggcatcacag catattcggc ataaccacct agattgacta atccgtatac ttttgtccca
     6601 ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc
     6661 aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg
     6721 acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact
     6781 tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat
     6841 ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag
     6901 tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac
     6961 tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt
     7021 aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg
     7081 tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca
     7141 ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt
     7201 ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc
     7261 ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc
     7321 cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg
     7381 ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat
     7441 caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct
     7501 ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg
     7561 ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca
     7621 tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac
     7681 cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg
     7741 ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag
     7801 tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt
     7861 ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc
     7921 tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa
     7981 aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa
     8041 agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt
     8101 tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat
     8161 agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt
     8221 ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc
     8281 taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg
     8341 atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt
     8401 cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt
     8461 tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg
     8521 cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt
     8581 tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg
     8641 cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac
     8701 gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa
     8761 gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca
     8821 gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc
     8881 tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga
     8941 ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca
     9001 atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc
     9061 aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt
     9121 tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt
     9181 gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg
     9241 ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt
     9301 gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc
     9361 gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc
     9421 cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt
     9481 cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt
     9541 ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt
     9601 ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt
     9661 gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag
     9721 gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt
     9781 tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat
     9841 agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta
     9901 cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg
     9961 caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc
    10021 cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt
    10081 tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca
    10141 tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc
    10201 ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac
    10261 gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg
    10321 ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat
    10381 aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg
    10441 ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga
    10501 cactcctttt aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt
    10561 ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc
    10621 aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat
    10681 ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg
    10741 gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca
    10801 caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga
    10861 gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact
    10921 ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat
    10981 catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg
    11041 aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg
    11101 tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc
    11161 ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa
    11221 caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa
    11281 tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt
    11341 agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg
    11401 aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat
    11461 agctggattc accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga
    11521 tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca
    11581 tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga
    11641 tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg
    11701 ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt
    11761 aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg
    11821 tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt
    11881 tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa
    11941 agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt
    12001 cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct
    12061 agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc
    12121 agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact
    12181 agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga
    12241 tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt
    12301 aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga
    12361 attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc
    12421 aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta
    12481 atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta
    12541 tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt
    12601 ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg
    12661 aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt
    12721 aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat
    12781 tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca
    12841 acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga agagatgaat
    12901 ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga
    12961 gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct
    13021 tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga
    13081 acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt
    13141 gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga
    13201 ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta
    13261 ttcctatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg
    13321 aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg
    13381 atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc
    13441 atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg
    13501 ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc
    13561 ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat
    13621 attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac
    13681 gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat
    13741 atcattttgc cttatctttc tccaggaatc atagctggat attttatggc atttacctat
    13801 tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca
    13861 gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg
    13921 ttagttttct tgttctccat gatattggtc attggatact actttattag taaagaaaac
    13981 cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga
    14041 aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg
    14101 ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact
    14161 ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag
    14221 tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga
    14281 caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac
    14341 tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt
    14401 tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag
    14461 ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt
    14521 ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc
    14581 tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag
    14641 cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca
    14701 aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg
    14761 atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt
    14821 ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca
    14881 tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta
    14941 caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct
    15001 atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc
    15061 taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact
    15121 cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg
    15181 agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt
    15241 tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga
    15301 gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat
    15361 gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa
    15421 tatttgtagc tatgaa
//
LOCUS       DAYYHB010000058        15302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000058 DAYYHB010000000
VERSION     DAYYHB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.059"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..252)
                     /locus_tag="ACHA6B_001824"
     CDS             complement(22..252)
                     /locus_tag="ACHA6B_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949392.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="ACHA6B_001825"
     CDS             complement(688..1254)
                     /locus_tag="ACHA6B_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8949393.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="ACHA6B_001826"
     CDS             complement(1254..2210)
                     /locus_tag="ACHA6B_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8949394.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="ACHA6B_001827"
     CDS             complement(2210..2866)
                     /locus_tag="ACHA6B_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8949395.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="ACHA6B_001828"
     CDS             3236..5254
                     /locus_tag="ACHA6B_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8949396.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /gene="leuS"
                     /locus_tag="ACHA6B_001829"
     CDS             5681..8095
                     /gene="leuS"
                     /locus_tag="ACHA6B_001829"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8949397.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="ACHA6B_001830"
     CDS             8443..9996
                     /locus_tag="ACHA6B_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8949398.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="ACHA6B_001831"
     CDS             complement(10377..12161)
                     /locus_tag="ACHA6B_001831"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949399.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="ACHA6B_001832"
     CDS             complement(12176..13897)
                     /locus_tag="ACHA6B_001832"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949400.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="ACHA6B_001833"
     CDS             13997..14758
                     /locus_tag="ACHA6B_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8949401.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4981 a         2799 c         3001 g         4521 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aa
//
LOCUS       DAYYHB010000059        15216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000059 DAYYHB010000000
VERSION     DAYYHB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.060"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHA6B_001834"
     CDS             278..787
                     /locus_tag="ACHA6B_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8949402.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHA6B_001835"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHA6B_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8949403.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHA6B_001836"
     CDS             2322..3302
                     /locus_tag="ACHA6B_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949404.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHA6B_001837"
     CDS             3424..4842
                     /locus_tag="ACHA6B_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8949405.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHA6B_001838"
     CDS             4913..6382
                     /locus_tag="ACHA6B_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8949406.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHA6B_001839"
     CDS             6392..6859
                     /locus_tag="ACHA6B_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8949407.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA6B_001840"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA6B_001840"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8949408.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACHA6B_001841"
     CDS             7849..10383
                     /locus_tag="ACHA6B_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8949409.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACHA6B_001842"
     CDS             10539..11312
                     /locus_tag="ACHA6B_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8949410.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACHA6B_001843"
     CDS             11305..12096
                     /locus_tag="ACHA6B_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8949411.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACHA6B_001844"
     CDS             12106..12597
                     /locus_tag="ACHA6B_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8949412.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACHA6B_001845"
     CDS             12615..13025
                     /locus_tag="ACHA6B_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8949413.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACHA6B_001846"
     CDS             13029..14429
                     /locus_tag="ACHA6B_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8949414.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACHA6B_001847"
     CDS             14452..14826
                     /locus_tag="ACHA6B_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8949415.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5282 a         2132 c         3044 g         4758 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattacg attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaa
//
LOCUS       DAYYHB010000060        13888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000060 DAYYHB010000000
VERSION     DAYYHB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.061"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHA6B_001848"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHA6B_001848"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8949416.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHA6B_001849"
     CDS             complement(2262..2513)
                     /locus_tag="ACHA6B_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8949417.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHA6B_001850"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHA6B_001850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8949418.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHA6B_001851"
     CDS             3463..3681
                     /locus_tag="ACHA6B_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949419.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACHA6B_001852"
     CDS             complement(3970..4482)
                     /locus_tag="ACHA6B_001852"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8949420.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHA6B_001853"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHA6B_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8949421.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACHA6B_001854"
     CDS             complement(6935..7378)
                     /locus_tag="ACHA6B_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8949422.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACHA6B_001855"
     CDS             complement(7379..8167)
                     /locus_tag="ACHA6B_001855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8949423.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHA6B_001856"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHA6B_001856"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8949424.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACHA6B_001857"
     CDS             complement(9282..9869)
                     /locus_tag="ACHA6B_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8949425.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHA6B_001858"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHA6B_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8949426.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11700..12710
                     /gene="gap"
                     /locus_tag="ACHA6B_001859"
     CDS             11700..12710
                     /gene="gap"
                     /locus_tag="ACHA6B_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8949427.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12795..13730)
                     /locus_tag="ACHA6B_001860"
     CDS             complement(12795..13730)
                     /locus_tag="ACHA6B_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949428.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGCFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3839 a         3106 c         2448 g         4495 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag agttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
    11701 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
    11761 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
    11821 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
    11881 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
    11941 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
    12001 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
    12061 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
    12121 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
    12181 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
    12241 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
    12301 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
    12361 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
    12421 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
    12481 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
    12541 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
    12601 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
    12661 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
    12721 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
    12781 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
    12841 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
    12901 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
    12961 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
    13021 gcttcaccga aaaagcagcc agcacggact tttgtcacta gactcgttac ttgcataagc
    13081 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
    13141 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
    13201 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
    13261 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
    13321 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
    13381 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
    13441 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
    13501 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
    13561 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
    13621 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
    13681 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
    13741 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
    13801 aatgttcatt tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt
    13861 atggtacact caaattgtat ttccgacg
//
LOCUS       DAYYHB010000061        13445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000061 DAYYHB010000000
VERSION     DAYYHB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.062"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(307..1440)
                     /locus_tag="ACHA6B_001861"
     CDS             complement(307..1440)
                     /locus_tag="ACHA6B_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8949429.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="ACHA6B_001862"
     CDS             complement(1431..2099)
                     /locus_tag="ACHA6B_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8949430.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="ACHA6B_001863"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="ACHA6B_001863"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8949431.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="ACHA6B_001864"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="ACHA6B_001864"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM8949432.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="ACHA6B_001865"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="ACHA6B_001865"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM8949433.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="ACHA6B_001866"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="ACHA6B_001866"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM8949434.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="ACHA6B_001867"
     CDS             complement(5475..6248)
                     /locus_tag="ACHA6B_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8949435.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="ACHA6B_001868"
     CDS             6413..6892
                     /locus_tag="ACHA6B_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8949436.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="ACHA6B_001869"
     CDS             6949..7251
                     /locus_tag="ACHA6B_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8949437.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="ACHA6B_001870"
     CDS             7273..8730
                     /locus_tag="ACHA6B_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFM8949438.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(8777..9004)
                     /locus_tag="ACHA6B_001871"
     CDS             complement(8777..9004)
                     /locus_tag="ACHA6B_001871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949439.1"
                     /translation="MRSDSGKKKSRNSQLNNKKSKSTKQTRTQKESLRNTNKGKLKGV
                     KKSINVDNFFIGCGKRWAMLFEMDFTSIYFL"
     gene            complement(9323..10075)
                     /locus_tag="ACHA6B_001872"
     CDS             complement(9323..10075)
                     /locus_tag="ACHA6B_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8949440.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10517)
                     /locus_tag="ACHA6B_001873"
     CDS             complement(10290..10517)
                     /locus_tag="ACHA6B_001873"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFM8949441.1"
                     /translation="MYQVFEEMGYPIKTVTRKLEVIKADDTISDLLDVEEGDPMFYFH
                     TQGFSLERLPVEYSISKYRGDINYFVFELAQ"
     gene            complement(10536..11494)
                     /locus_tag="ACHA6B_001874"
                     /pseudo
     CDS             complement(10536..11494)
                     /locus_tag="ACHA6B_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11700..13382)
                     /locus_tag="ACHA6B_001875"
     CDS             complement(11700..13382)
                     /locus_tag="ACHA6B_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8949442.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
BASE COUNT         3931 a         2901 c         2267 g         4346 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    10681 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcatt tagccctctt
    10741 tgcgaaggtg ttcctggatc tgcgaaaaag atggcaatat cgttcgagtt acttatagat
    10801 cgccagttgg aaaattcttt tccacaatca aatgtcatgg atttaaacaa gtgtttaggc
    10861 atggatttga accaatcatt taacctgttt tcgatatctt cggctcgtct gcctgtcggt
    10921 ttcaaagtaa taataacctt ggataaacac tcaaccaatg taataacagc gctcttgtgt
    10981 ttaactccca caatggtgtc gccttcgaga tgaccaaatt ctttagtcag gtgtggatag
    11041 agactgttcc gttcatgaat cgttctgcga aaggcctgtt ttccacgctt ttcttgatga
    11101 ttattcggtt tacgtttccc cttcataggt aacgactgtg tgtcaaaaag gcctttttta
    11161 aacatccgat agagtgtacg aacagaacaa tcaattggaa actcgccacg gccgataatc
    11221 acatcgggtg accacccttg agctaccttt tcttgaacat atgttgattg ttcatcagat
    11281 aaaacggttg aacgtcgtcc acaacgagat ttattttgtt tgtattgacg gtaataatct
    11341 agcacttttt tccttgtttg agaaacgaat aaacattata aatggtttgt ctagagcgat
    11401 ttaaactttg tgcgacaaga gcgacaggtt tctcgatatt gaaataagac tctatgatta
    11461 ctagttcgtc tgttgtaaga tgggtgtagg tcatttgtac tcactcctta ttaattcttt
    11521 ggtcggaact attttgagtg tagcacaaat gatcttttta gttgtctagc ttaattttac
    11581 aatcgacgcc tcaaaaagaa tacccgaaat ttaaacagca aaaaaagata gcgcttacca
    11641 ttgtgtttaa gataaaacgc tagctaagtt ggctagcgct ttattaatct aagcaagctc
    11701 taaaattgct tcaatacaaa atcaactgct cctttaggat cacgtgttaa attgatatat
    11761 tctgcacctt tagttgcaat tacttttatc cctaatttgt ctgcatcttt acggagatca
    11821 tccaaatagg aattcacttg aggtgctaga acgattaaat caaaattttt catcatatca
    11881 taatgagaac cataagctcc tgctttagca gaaagattaa cacctgtctc ttttgcgccc
    11941 tcttctaatg cattagccaa cattgcactc gttccggcac cagcacaaag tactaaaacg
    12001 cgctttcctt caccaaccga tgcttttgct tcatcaacta taactgccgc agttgcacca
    12061 acttcttgga tttcttcgac caattcttct tttacttctt ctgcagcaat gttttttaac
    12121 tcttgttcta ctaattcaga atcataggcc ttaacaaaag gtaaataaat aatcgcatcg
    12181 acaacaatca atactaccgc taatacaatc gccaacacac taaacccggt tcctaaaacc
    12241 aagccaatcg gtgcgggcgt cgcccaaggc aatacggata taaagctatt catccccaag
    12301 acatcgacaa aaagtttgaa tatccatacg ttagcgattg gtgctaataa aaatggaatt
    12361 agaaagtacg gattcaaaat aataggagta gcaaatagta acggttcatt taccgcaaac
    12421 gaaactggaa taaatgaagc tttaccaact gctttcaatt gttttgattt agcgaacaac
    12481 atgaacagaa aaggaacaac tagtgtcgct ccagtaccac ccatcgttcc aacaaagttt
    12541 cctagaccaa ctgttaatac ttttgaggct tgtgcaccgt ttttaaataa ttcaaagtta
    12601 gtttctacgt tcgcataaat aattgcagca atcgctggtt caacaatcga tggtccatga
    12661 acaccaacaa accagaacat tgccatggct ccccaaatga ttgcaatacc gacatatcca
    12721 tcagcagcac taaataatgg ctgcaataat tgaattacac ctgctgcaaa tgatgttcca
    12781 aaaaattgac gaactcccgt atcaatcaaa acggcaataa atactgcaat tgaaaagggg
    12841 ataatatctc taaacatctg tgtgattgtt ccgggaactt cttttggcat acggatcgtc
    12901 aaatctcttt taacacagaa tttatacaca tttacagtaa caaacgcaga aacgaaagct
    12961 gctaataatc cctttgtccc taaataatca gtagctatcc caccatcaat ttgatcagct
    13021 gccatcatta caaaaccaca aattgacgct aacataacag aaacttcatt aatctgctta
    13081 cctgaatcag ataattgacg gttcattgaa ttagctaaac atcgagctgt tgtagcaaca
    13141 accaaaagac caactaatcc cattgaataa ccataaactt tccaaagcca cgtcgagaat
    13201 gcctctggta acgtcacgtt aaatacttct ggaacagccg caatcataat aaaaatactt
    13261 gaaaatagaa tagctggcat agcagttaaa aagccatcgc gaattgcccc taaataaata
    13321 ttacgtgaga ttttttcaaa aaacggtttt cccttctcaa tttgcttaat aatcgtattc
    13381 atttttcttc tcctatgctt tagcaaattt ttctttgtac aattcaacaa aatcattgat
    13441 aagtt
//
LOCUS       DAYYHB010000062        13397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000062 DAYYHB010000000
VERSION     DAYYHB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.063"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..494
                     /locus_tag="ACHA6B_001876"
     CDS             195..494
                     /locus_tag="ACHA6B_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949443.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(591..1025)
                     /locus_tag="ACHA6B_001877"
     CDS             complement(591..1025)
                     /locus_tag="ACHA6B_001877"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949444.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRGKKVLFGNDK"
     gene            complement(1257..1967)
                     /locus_tag="ACHA6B_001878"
     CDS             complement(1257..1967)
                     /locus_tag="ACHA6B_001878"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8949445.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1957..2817)
                     /locus_tag="ACHA6B_001879"
     CDS             complement(1957..2817)
                     /locus_tag="ACHA6B_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8949446.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2822..3460)
                     /locus_tag="ACHA6B_001880"
     CDS             complement(2822..3460)
                     /locus_tag="ACHA6B_001880"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8949447.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3475..3774)
                     /locus_tag="ACHA6B_001881"
     CDS             complement(3475..3774)
                     /locus_tag="ACHA6B_001881"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949448.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3817..5301)
                     /locus_tag="ACHA6B_001882"
     CDS             complement(3817..5301)
                     /locus_tag="ACHA6B_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8949449.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5321..5782)
                     /locus_tag="ACHA6B_001883"
     CDS             complement(5321..5782)
                     /locus_tag="ACHA6B_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8949450.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5800..6867)
                     /gene="ulaG"
                     /locus_tag="ACHA6B_001884"
     CDS             complement(5800..6867)
                     /gene="ulaG"
                     /locus_tag="ACHA6B_001884"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8949451.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7085..7855
                     /locus_tag="ACHA6B_001885"
     CDS             7085..7855
                     /locus_tag="ACHA6B_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8949452.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8128..8955)
                     /locus_tag="ACHA6B_001886"
     CDS             complement(8128..8955)
                     /locus_tag="ACHA6B_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949453.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9522..10757
                     /locus_tag="ACHA6B_001887"
     CDS             9522..10757
                     /locus_tag="ACHA6B_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8949454.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11756..12016)
                     /locus_tag="ACHA6B_001888"
     CDS             complement(11756..12016)
                     /locus_tag="ACHA6B_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8949455.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12043..13344)
                     /locus_tag="ACHA6B_001889"
     CDS             complement(12043..13344)
                     /locus_tag="ACHA6B_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8949456.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4260 a         2775 c         2112 g         4250 t
ORIGIN      
        1 aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg attatttcaa
       61 tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa atatacttgt
      121 cattcatcat gaaatataga gacttataaa attgtttatt tttataatgt atcagtatag
      181 aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc
      241 cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg
      301 agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga
      361 tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg
      421 tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta
      481 aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg
      541 tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca
      601 ttaccaaaaa gcaccttttt tcctctagta tctatgacat aagaaatacc acccatttcc
      661 tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc
      721 aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc
      781 actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg
      841 atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc
      901 tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa
      961 ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata
     1021 ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat
     1081 ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa atctctcttt
     1141 ccttttagaa tgcatccttt ttgataaata aaaaggttgt gataaatttt cttcacaacc
     1201 tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat
     1261 tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc
     1321 gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca
     1381 tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca
     1441 tgtccataga caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc
     1501 tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat
     1561 tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc
     1621 aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca
     1681 gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt
     1741 aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc
     1801 atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact
     1861 tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct
     1921 tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc
     1981 cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct
     2041 tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct
     2101 taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga
     2161 atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc
     2221 tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg
     2281 atatacttgt agaaaagggg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga
     2341 gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc
     2401 ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc
     2461 ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg
     2521 gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa
     2581 cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat
     2641 ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc
     2701 cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg
     2761 ttctttccat gaaatatctt tgggtaatgc tttttcataa atcccaattt gagccatttc
     2821 cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat
     2881 ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt
     2941 gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt
     3001 ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc
     3061 tttgatggta gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag
     3121 tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag
     3181 ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac
     3241 tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga
     3301 acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca
     3361 cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt
     3421 cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt
     3481 gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttgg ctgacatcac
     3541 atttttgata ccgataactg taacttcttt tttctctgcc tgagtaaaca tattcaaaaa
     3601 attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc
     3661 acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct
     3721 catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat
     3781 ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata
     3841 tgtgtcatag tcttctacaa tcaaaaaata accctcagga tttttccgat attgtaactg
     3901 cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat
     3961 gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccata
     4021 tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga
     4081 aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat
     4141 aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa
     4201 taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt
     4261 taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc
     4321 tggtaaaatt gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg
     4381 aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta
     4441 gaataagaaa ctctgtcctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag
     4501 tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc
     4561 attgaaaatc gacaagaatc ctggtaattt gatatcttcg atttttttgt cttgcttgat
     4621 tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa
     4681 gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt
     4741 gattcctact gttaagttag aagctactgc ccaataacaa cctaaaatca gtcccataaa
     4801 aatcaatact tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt
     4861 agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt
     4921 ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc
     4981 tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata
     5041 aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca aaatcggccg
     5101 aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt
     5161 taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac
     5221 gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat
     5281 gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca
     5341 caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca
     5401 ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcaa
     5461 gtgtaaaaaa caaactagcc gttttctcct catcctcccc ttcactttca aaacggattg
     5521 gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag
     5581 aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat
     5641 ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc
     5701 tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat
     5761 ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg
     5821 tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc
     5881 atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc
     5941 atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg aatcagccat
     6001 aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc
     6061 catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata
     6121 ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta
     6181 atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc
     6241 aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt
     6301 gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac
     6361 tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt
     6421 gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc
     6481 ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt
     6541 ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg
     6601 aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg
     6661 ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca
     6721 aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc
     6781 gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt
     6841 tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt
     6901 acaagtaaat attaatgtgt atcagcatcc atggaaaccc ttttatatga ccgtttgttt
     6961 tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa
     7021 tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa
     7081 agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct
     7141 tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg tatctattag
     7201 tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg
     7261 ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat
     7321 taaacaagcg attgccagag aagctagtaa atacattaac gattatgata cgctgtttat
     7381 caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt
     7441 cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt
     7501 aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac
     7561 gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca
     7621 tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac
     7681 aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa
     7741 gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt
     7801 attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc
     7861 aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg
     7921 aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtgtccatc caatcgatac
     7981 catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa
     8041 tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca
     8101 aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc
     8161 gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc
     8221 atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata
     8281 agtcgattta tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc
     8341 ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag
     8401 attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa
     8461 atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg
     8521 gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg
     8581 ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc
     8641 agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt
     8701 tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt
     8761 ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt
     8821 gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc
     8881 ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc
     8941 atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata
     9001 cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaataaaaat tttaatttta
     9061 aaataatatt caaattaaac aactggcgta tctgctcctt gatttctttt tgtgttatct
     9121 gaataattat cgtttacagt tataacacat agtgaaaatt gtcatggcga gaaagtctaa
     9181 gaatattttt aaacataact atttctttct tacgtgtgta taatgacgac agcacaccta
     9241 tcaattaata taattatttt attttttatg ttctcatgtt tatttttaat gacacaactt
     9301 ctcgtattta tctggcacaa catataaatc gatcaaaaaa ttgttgcgtg tttttttgaa
     9361 aattggagtt tcaacttgat tacaaaattt aaatttagaa gttgtgaaaa cagcgagaga
     9421 cagtttaata ttcaaaatat acataggcta ctcgaacgct gtgtttttta gtatactagt
     9481 aagaaacgaa gggatagact cattccaata aggaggattt catgaagaca attctttttg
     9541 ctcctgcagt attcaattta gctgagacaa cacgaatgat tgaggttgcc aaagctatga
     9601 aagaccgtta ttactgtgta ttctttggct tttctaaaac ttttgctcct ttgatcacag
     9661 aaagtggctt tgagtatcat ttactagcgc cgatccttac gaaaaaacaa gaagaacaaa
     9721 tcataaaatt tgaccagttg cgtacactta aaaacccttt taccaaagaa atggttgcct
     9781 ctcgtatcac tagtgaacag agtctgattg ctaagtttga gcctcgactg attgtgacag
     9841 gttcgaatgt gacaattttt ttatcggccc gcaataaaaa gattccttta gtatatgtta
     9901 aaccctatgc tttgagtgaa cctttttttc aatcagagca tgcgctgatg ccccacgagt
     9961 tacgtttctt cggcccttta agcaatcccc tttggcactg gacaaaaaag cgtatagtaa
    10021 atacccgatg gatccccaat gccttcaaac acgctgcaca aaaagaaatg cttcctttac
    10081 caaaaacaag tttacaaatg atggatgctg atctaaatct catcacaacg cctaaactat
    10141 ttttggaaga agaacagctg ccggaaaatt atcgaatcgt tggtccaatt tttgctaagc
    10201 tcagtacttc cttgcctgaa gaagtgctaa cttttataca gcgaaagcaa caagaaagaa
    10261 aacgtattat ctatttcgca atgggtagtt caggcaatcc gcgtatgtta cgcaaaattt
    10321 tgttcttctt acaaaaacaa ccggatctag cggttattgc acctattgac tatctgcttg
    10381 atgacattca atctgactca gaagatcttt taataacaga atatctgtct agtcatcttt
    10441 tgcatgaatt cattgatttt tcttttatcc acggcgggga agggaccgtc caaactgcct
    10501 gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt tgagcaaagc tgtaatattg
    10561 actattgtgt aaaatacgga aacgcaatcg ccctcacaaa accagtgact gaaagaaaac
    10621 tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg tcagcaggca cgcacgctgc
    10681 aagccgtttt tccaataaat ggtccacaac aagcaattca agaaatcgaa accttcctga
    10741 gcaaccaccc aatatagttt caagtagaaa aagctttttt aatagagact catcagtaat
    10801 ttcgctattc gagacccaga cagtgaaaat acaaaaaaag taaatagtgg attgaaaagt
    10861 tagagttact taaattacgg tgtctaaaaa acacaaaatc atccttaaaa taaaagatct
    10921 gacgcaatcc aaatgagcat aggtagcgtc tgccatttat tcttagtcaa attctttgac
    10981 acctttctct ggttggatca tctcaacgtt tcttcaaaag aaagaccata ataattaggc
    11041 aaatacattt tcttaaaatg tgtcatatag atctagaagc atcgctttgt agagatttgt
    11101 gctgctagtc ccttcaacaa ttactaaatc aaatgtagga tttaccactt tatctttgat
    11161 aatcaacctc cctcttctgt tgagacaatg atctcgcaat ccgatgcata ctcctatata
    11221 aactgccagc aatccccaca tgctgagtag atcatgtcag aatgttctat gtcgtcagca
    11281 acgataaact aaaagtcacg ttcaccatga ctgattgcta ttcccagcgc aattatttct
    11341 atacacgttt catgatttga ataaacactc tcatcataca tattaccatt catacaccat
    11401 actgcagccc ctactatatg gttgtaagct cccaaatcta tatttgcctt gatcactttg
    11461 gtagtagatc gactaattct taatccattg ctgataaaat tttcattttt gcctccttct
    11521 ttctgccaaa acagttaaaa caataataat ctttatgtaa aaaagggctg tgacaaaagc
    11581 cttgtcacag tcccatattc cgaataaacg gtggtcaaaa gcttgcatct gcggtaataa
    11641 gttcaaccaa acgaaaaata agccaacatt tcacaaaaat ttaagaagca attttcgtaa
    11701 atttcttctt atttcttgaa gctaaccact tctgtcacaa ccacttttag aacatttaaa
    11761 gcttcgatgt gacaaaccat ctatgctgct cgatcataaa attgatagca aaaattccac
    11821 aaagtacata tgtcccccat gaaaacaaaa cattcatatg cggtacaatg atctttggaa
    11881 gaatcacgat caaaaatgat gccagtgccc aaatccatcg attgatttga atatgatatg
    11941 tagctagcat attcaatata aggtgactag ccgttaataa aaagataaaa acaaaaaata
    12001 aaatcagcca atacattgtt tcccaccttc taaaaaccaa acttagacag tttggacttc
    12061 gttttctcca cgactttgtt catctgacag catcttttta tcataagctt ttacaaatgg
    12121 ataatagata ataaatgcta tcaatgtgtt gatcatgatc aagataacaa cacgccagtc
    12181 atttcctgct gaaagataac cgccgattgg agctggtaat gtccaagttg gcaaaacagt
    12241 aaatctattg accatttcta atttgactgc aaaataactg ataatagtca aaactaaagg
    12301 aactaagttg aacgggatga acatgtaagg attcaacatc ataggagctc caaaaatcat
    12361 gggctcattg atattgaaga taccaggaat aatagaaaaa cgaccaacct ctttcaagaa
    12421 ttttgattta cagaagaata taaacaaaac agataccacg atcgttgctc ctgcaccacc
    12481 aattgttacc gtccattgat aaaactgttc aggagcgata tagggtaatt gggtggcagc
    12541 tgtaccatct gccaatgcct ttgcattctc atccaacatg atcagccaca ttggacgaac
    12601 aacagcccct acaatactca tgccatggat accaaatgac cacaacaaat ggatcaaaaa
    12661 cattggtagt aaaacaccaa gcaaattatt ccctaaaata ttggttaaag gctcgaataa
    12721 cgataataag gtagcgttta catcaaagcc cgcgacgctt cgaatcagcc aagtaattgt
    12781 taaaatgact gcacctggaa tcaatgcctc aaaactacgg gcaacagcag gtggcacttg
    12841 ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa cgatatactt ccactgcaaa
    12901 aatcatagat aagatccccg caaacatccc agatgctccc aaagaactca taggtaagac
    12961 ccagccaaga ggtgcctttt caggagttaa aacgtttaca ggaacttgta acataaagaa
    13021 cgttcctaaa gataatatac cacctgtcac actatctaat ccatatgatt tagctagatc
    13081 cgaacccata acaaaagttg cataaatagc catcaatccc atcgtcatcc taaaaggtaa
    13141 aacaatcgtt gccgaataag gggcaataaa ttcattccat ccaggaaacg gtaaattgat
    13201 aaagaccata aaaaatgtcc caatcaaaat caatgacaat gttgaaacaa ccccattacg
    13261 aattgctaat aaatgacgct gtgtaccaat ttttgccatt ggtgggacaa gtttctcttc
    13321 gacccatttc atcagctttt ccactaaaac cactcctttt caataaaata tgaaccattc
    13381 attgcatgac aacgcaa
//
LOCUS       DAYYHB010000063        13204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000063 DAYYHB010000000
VERSION     DAYYHB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.064"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..206)
                     /locus_tag="ACHA6B_001890"
     tRNA            complement(134..206)
                     /locus_tag="ACHA6B_001890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Lys,seq:ctt)
     gene            587..1156
                     /locus_tag="ACHA6B_001891"
     CDS             587..1156
                     /locus_tag="ACHA6B_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8949457.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1143..2003
                     /locus_tag="ACHA6B_001892"
     CDS             1143..2003
                     /locus_tag="ACHA6B_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8949458.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2193..2897
                     /gene="yycF"
                     /locus_tag="ACHA6B_001893"
     CDS             2193..2897
                     /gene="yycF"
                     /locus_tag="ACHA6B_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8949459.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2902..4737
                     /gene="walK"
                     /locus_tag="ACHA6B_001894"
     CDS             2902..4737
                     /gene="walK"
                     /locus_tag="ACHA6B_001894"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8949460.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4734..6050
                     /gene="yycH"
                     /locus_tag="ACHA6B_001895"
     CDS             4734..6050
                     /gene="yycH"
                     /locus_tag="ACHA6B_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8949461.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6051..6920
                     /locus_tag="ACHA6B_001896"
     CDS             6051..6920
                     /locus_tag="ACHA6B_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8949462.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6975..7784
                     /locus_tag="ACHA6B_001897"
     CDS             6975..7784
                     /locus_tag="ACHA6B_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8949463.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7887..8516)
                     /locus_tag="ACHA6B_001898"
     CDS             complement(7887..8516)
                     /locus_tag="ACHA6B_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8949464.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8686..9978
                     /locus_tag="ACHA6B_001899"
     CDS             8686..9978
                     /locus_tag="ACHA6B_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8949465.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10054..10716)
                     /locus_tag="ACHA6B_001900"
     CDS             complement(10054..10716)
                     /locus_tag="ACHA6B_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8949466.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10941..11225
                     /gene="groES"
                     /locus_tag="ACHA6B_001901"
     CDS             10941..11225
                     /gene="groES"
                     /locus_tag="ACHA6B_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8949467.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11276..12901
                     /gene="groL"
                     /locus_tag="ACHA6B_001902"
     CDS             11276..12901
                     /gene="groL"
                     /locus_tag="ACHA6B_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8949468.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4389 a         2273 c         2843 g         3699 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaaataa
       61 aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg
      121 gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagtcagc
      181 tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt agcactaaga
      241 attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt ccttatagga
      301 aatcattcat agattattca catttgctcg ctattatata agcataccaa caaacaaagt
      361 tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc cgccgtctcc
      421 ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc ttgttttttg
      481 ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg aatgtttatc
      541 ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg
      601 tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc actgggaaga
      661 actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc gttatctttc
      721 acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa acaattacgt
      781 taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac acgtagcatt
      841 tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca aagaagggat
      901 attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt gcgatgaaca
      961 agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt
     1021 cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat cgtttgcaat
     1081 gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag aggagaaaac
     1141 aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtg ccagaagaat
     1201 tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa gatcaggtat
     1261 atacagataa aattgacatt acctctgaag agatctatca acgtttgcca gaggaaatcc
     1321 ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag
     1381 acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg acatataatg
     1441 tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta gatacgaaaa
     1501 atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg gaagaaggag
     1561 tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca aaagttttct
     1621 ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta gttgcttcta
     1681 ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa gggatttatt
     1741 acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca
     1801 aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg gatgcgaaag
     1861 aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca aaccaaatct
     1921 actttggcca gatttctccg gcattagtag tccacactgg tccgggatta ttgggtgtcg
     1981 gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt
     2041 ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt tcattttaat
     2101 tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta taatgagaag
     2161 gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt gtcgatgatg
     2221 agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat gatgtttata
     2281 ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct gatttgattt
     2341 tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa
     2401 cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat aaagtcctag
     2461 gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga gaattagttg
     2521 cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag
     2581 cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac atggtgacga
     2641 aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt tacttggcga
     2701 agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg ggttatgatt
     2761 attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag
     2821 ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac tatttgcgta
     2881 acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc agtcaatttt
     2941 aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat tggtgcctac
     3001 tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat gaatcaaacg
     3061 gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa tgcagatgat
     3121 gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag cgatatcatc
     3181 gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt caatgaacaa
     3241 agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac taaacgttat
     3301 gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat cctgtccccg
     3361 accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca aaaatattcg
     3421 gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc atttatttca
     3481 atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga aatgagagaa
     3541 caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca tggacaagat
     3601 gaacttggac agctggctga taccttcaat cagttagctg aacgtattga agaagctcaa
     3661 gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat gacagacggc
     3721 gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc tgtttccttg
     3781 ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt agatatcgaa
     3841 gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact tgaccgttca
     3901 accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat gatccgtcgc
     3961 gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga acaagaaaaa
     4021 acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg tacaccgttg
     4081 acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta
     4141 gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg aatgatcaat
     4201 gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt agaatatgtc
     4261 aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt gggaaatcag
     4321 gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt cgaagtagat
     4381 acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa gtattcacca
     4441 gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtggt tttaagtatc
     4501 accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga gcggttctat
     4561 cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt agctatttct
     4621 agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga aggaaaaggg
     4681 tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg ggaatgagaa
     4741 tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc atatacttct
     4801 cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat gagacaaaca
     4861 ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt ccgcttcatg
     4921 ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc gtgtaccgat
     4981 tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt tcaggagaca
     5041 aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc tataatgcgc
     5101 cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg tcgaacttgg
     5161 caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga aataaaaaaa
     5221 tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg ttgaattgga
     5281 cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg acaacagaac
     5341 catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaaa aagtatagtt
     5401 atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag aaaccagata
     5461 gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat gaaacaatgt
     5521 ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa
     5581 atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt tctttaggga
     5641 acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc gtggaaggct
     5701 tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc ggtgaaacga
     5761 atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg ccgatacctt
     5821 cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta aaagctaagg
     5881 gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa aatgtcaaag
     5941 aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa gataactggt
     6001 attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa ttggatttca
     6061 aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca
     6121 gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag actgaatcaa
     6181 tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg tctgagaaca
     6241 agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta gcgaattatc
     6301 gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt gatgttttga
     6361 cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat aaaattagta
     6421 gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat gattatgtgt
     6481 acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca agccaagagt
     6541 atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta gaaaaaaacg
     6601 ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc gagcagctgc
     6661 gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac atcaataaca
     6721 aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt attctgaaag
     6781 tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca agcgataaag
     6841 tggtccaaat tgagagtgtc aacgctttca gcaatacgat cgtgacaaac aatactgtac
     6901 caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg
     6961 gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg
     7021 gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag
     7081 gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca ccggaccaat
     7141 tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg ggtgtcttgg
     7201 ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg atggaaccac
     7261 tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa gtcagaacat
     7321 tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct ccgcaatttt
     7381 atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga tattgcagtg
     7441 attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt aatcatgatc
     7501 ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt ttaggagata
     7561 gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga
     7621 ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa cttgctcatt
     7681 taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat tttgaaatct
     7741 atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt caatcaataa
     7801 aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta tttttatttt
     7861 tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg ctgcttttca
     7921 tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat gctgatcgta
     7981 ctgtcgccag cagcgtttct ttgtaaataa tcatcactta acaaataagt acctgtacgt
     8041 ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag atttttagat
     8101 tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa gggtgcattt
     8161 ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa cgaagaggca
     8221 cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa
     8281 gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac gacaacatca
     8341 aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa cggaacatct
     8401 gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct ggacaatgct
     8461 tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat tttcataaaa
     8521 aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc aaagaatccg
     8581 atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg tatagttaaa
     8641 ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa agagtggtta
     8701 gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt aactgctttg
     8761 cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt tttcttattt
     8821 gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt ttctatgtat
     8881 tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt ttttgccatt
     8941 gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat aacagccttg
     9001 tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt ctggacaggt
     9061 gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg ctatgcacgt
     9121 ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa tgtttggcag
     9181 tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat cggttcagcg
     9241 gtttttcaac agttgttcag aatgactgtt gttcaaggag tatggacata tgtccagctt
     9301 cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt ttttgttccg
     9361 tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt tagtcccgtc
     9421 ttgcaagcga attcattagc gaatgcagaa atcttagcct caggacaaga gttgacggtg
     9481 gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac ggttacgggt
     9541 gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc gaagaaatac
     9601 agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga atcaattatt
     9661 ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt cgcaccggtc
     9721 gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt atttacagca
     9781 gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg cggagtgcct
     9841 gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt cccatttttg
     9901 aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga tggagcagca
     9961 gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc
    10021 aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt ggacagctaa
    10081 ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg tccagatccg
    10141 accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg
    10201 accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga cccaaaaatt
    10261 tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt ccatgatggg
    10321 tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct ggatgatatt
    10381 ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca tgacagcgat
    10441 gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa tctttggagc
    10501 agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa aataaaaaat
    10561 agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca attcagacgt
    10621 agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta gaccatagca
    10681 aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt taagggataa
    10741 caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt tcaataagca
    10801 gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga gggagaatga
    10861 gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga ttaaaaaaag
    10921 agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg
    10981 caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac
    11041 cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag
    11101 ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag
    11161 taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg
    11221 agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc
    11281 aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt
    11341 agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc
    11401 ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga
    11461 agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga
    11521 tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg
    11581 tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc
    11641 aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc
    11701 cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga
    11761 tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga
    11821 aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat
    11881 ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga
    11941 caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc
    12001 acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt
    12061 gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg
    12121 tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga
    12181 tctaggttta gaattgaaag atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt
    12241 tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc
    12301 tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa
    12361 attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc
    12421 tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg
    12481 tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag
    12541 caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg
    12601 tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat
    12661 tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg
    12721 ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca
    12781 aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc
    12841 agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta
    12901 agaaaaagca acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct
    12961 ggaacagaag cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat
    13021 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttatacgtgt
    13081 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttttt gtgtgtaatt
    13141 ataatgaaaa tagttatttt taatagttat aatattttat ctattaaaag taaatatcca
    13201 ttca
//
LOCUS       DAYYHB010000064        13062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000064 DAYYHB010000000
VERSION     DAYYHB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.065"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..777)
                     /locus_tag="ACHA6B_001903"
     CDS             complement(115..777)
                     /locus_tag="ACHA6B_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8949469.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(787..1602)
                     /locus_tag="ACHA6B_001904"
     CDS             complement(787..1602)
                     /locus_tag="ACHA6B_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949470.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1864..2307)
                     /locus_tag="ACHA6B_001905"
     CDS             complement(1864..2307)
                     /locus_tag="ACHA6B_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8949471.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2338..2451)
                     /locus_tag="ACHA6B_001906"
     CDS             complement(2338..2451)
                     /locus_tag="ACHA6B_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8949472.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(2441..2779)
                     /locus_tag="ACHA6B_001907"
     CDS             complement(2441..2779)
                     /locus_tag="ACHA6B_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8949473.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2767..3144)
                     /locus_tag="ACHA6B_001908"
     CDS             complement(2767..3144)
                     /locus_tag="ACHA6B_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8949474.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3368..4561
                     /locus_tag="ACHA6B_001909"
     CDS             3368..4561
                     /locus_tag="ACHA6B_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8949475.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4727..5149)
                     /locus_tag="ACHA6B_001910"
     CDS             complement(4727..5149)
                     /locus_tag="ACHA6B_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949476.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5544..5738
                     /locus_tag="ACHA6B_001911"
     CDS             5544..5738
                     /locus_tag="ACHA6B_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949477.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5735..6229
                     /locus_tag="ACHA6B_001912"
     CDS             5735..6229
                     /locus_tag="ACHA6B_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949478.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(6391..7563)
                     /locus_tag="ACHA6B_001913"
     CDS             complement(6391..7563)
                     /locus_tag="ACHA6B_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8949479.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7769..8584)
                     /locus_tag="ACHA6B_001914"
     CDS             complement(7769..8584)
                     /locus_tag="ACHA6B_001914"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8949480.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8734..9180)
                     /locus_tag="ACHA6B_001915"
     CDS             complement(8734..9180)
                     /locus_tag="ACHA6B_001915"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8949481.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(9177..9692)
                     /locus_tag="ACHA6B_001916"
     CDS             complement(9177..9692)
                     /locus_tag="ACHA6B_001916"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8949482.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9765..10679)
                     /locus_tag="ACHA6B_001917"
                     /pseudo
     CDS             complement(9765..10679)
                     /locus_tag="ACHA6B_001917"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10676..13003)
                     /locus_tag="ACHA6B_001918"
     CDS             complement(10676..13003)
                     /locus_tag="ACHA6B_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8949483.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNEITSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3768 a         2540 c         2352 g         4402 t
ORIGIN      
        1 aagtatagca cgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg
       61 acagttttta cataaaaact gtcattctta attgaaagtt tcaatttata aagtctacag
      121 atccatagtt ctcatcaatt tacgattgtt gaagtaaatc aaaccaaata cgatagaagg
      181 acctagtaga atacaccatt ggggctttac gaaaaagaag agaaacaaac ttgtcaatag
      241 taaaaaatct agtaaactca cataccattt tttcaacgta tagtagaacg ctatccgtag
      301 aacttgattt ggtttcgacg ctggatttct cacctgaaaa taccagtagt tgatgctaaa
      361 ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc aaccattttc cataagaaaa
      421 acgtataaaa aagaaacaat caaaaatcat tatcacacta actatccaag caaatagcca
      481 ataaaaaaat ccctttagcc aaaattggcg aaacgtcttt atgaacaaac gtactggtga
      541 aaaacttttt tcttttgcta attcttctgt catacctatc atcacgatgc aacctggacc
      601 aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt ccatctatcg acaaaaggaa
      661 cgcagctgct atatacaaga tattgacaca ccaaaaacaa aggctacttg ccaatgattg
      721 atacaaccaa taaaagaatt ccatataaac atttttgtca aaacgctgct ctttcataat
      781 cacctcctat tccttcattc ctgatgaagc gatcccttct acaaaatatt tttgcatcgc
      841 taaaaatata aaagcaacag gaagaacaga aacaactgag gcagccatga ttaatgcgta
      901 ctctgcatca aatagcccaa taaacatttt caaccctaac tggattgttt tattttccgt
      961 agaagacaaa taaatgaatg gccccatata atcgttccat gtattaacga atgtgatgat
     1021 cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg atcttgcgat agattcccca
     1081 ctctgataag ccatcgatcc gcgcactctc acataaagaa tctgggatac tactgtagta
     1141 ttgcttcaac aagaacacac caaaagcatt gaacgcttgt aatagcacca acgaccacaa
     1201 cgtatatgtt aaacccactt ttctcatcat aatgaactgc gggatcatat aagactgcca
     1261 agggacagca atcgtgccga tatatgctaa aaataaaata tcgcgccctt taaaatttag
     1321 tttagaaaac ccgtaagcag caaaactaga agtaaacaac tgaatcaacg tgatcacaac
     1381 gcttaaaaaa gctgtattct taaaaaatgt cactaaagga atacgagacc aaataaccag
     1441 ataattctct aaatgccaag ttttagggat ccattgaatc ggaatcgtga acacttcatt
     1501 atttgctttg aatgaagcag aaaccatcca taaaaaagga atgatcgtca ataaagctaa
     1561 tacaatcagc agaatcatac ccaaccattg aataaagggc atgttttttg aacgaatttt
     1621 tgcttttttt ctttcatttc gaacacctcc aaagttataa tcttcatttc tttgtaatga
     1681 ttacaatcca gaacaacggt tattatagct gattcatttc cgttcttctt atgtttgtaa
     1741 gcgcttattt aacaagttat gtaaatctct ttattttttt tgatttaaaa atgaaaaaat
     1801 acaaaagcaa gactgtcata cgtctcgctt ttgtattgaa atttcttcga ttagagatac
     1861 ttattactgt tcaaaagctt ctttgaaacg gtgaatcaat tcattgacta caggtgataa
     1921 aatccgattc tttttccaga ctagcatgca gttcgtttca atcattgggt caagcggaac
     1981 gaatgtcact cgctttgaaa gatgacttac agcaccttct atgactaaag cagcacctac
     2041 ctgattctca attagcgcaa atacattgaa aatcaaattg tacgttcctg tgatcagcaa
     2101 ctgctccttt tttatgccta accagtcagc aaacattcgc tgtatttctg gacgctttga
     2161 gatcaaaaga ggtacattcg caaagtcttc ttttgttatc acgtcttttt ttgctaaaac
     2221 agaattttct aaaatgagca atccccattt ttcctttctt ggtaacaaaa tcgtttcgta
     2281 tttctctgta tctactggat ctagcaaaat cgctaaatct aacaggcttt tttctagtca
     2341 ctccgtgata tcatcacttg ttccgctgtg gatatgaaat tgaacgctgg gataatcacg
     2401 gatcaacttt tctagtaata gtgccaatgt attcgaatta tcagcttcca cacagccgat
     2461 cgtaaatcgg ccgctgaata attgtttttt tctgttctcg aattcttgag tggtttgatt
     2521 cgttaaatct aaaatttctt ctgctcggct tttttagaaa ataacctgct tctgtcaaga
     2581 tcattttgcg gttttcgcgt acaaacagtt ctgttcctag ttcctcttct aactccttca
     2641 gttgtcggct taaggttggt tgtgtaagat gtagaacttc tgcggcttga gagattgttc
     2701 cttcttctgc aattgtccaa aaatatttta gtaagcgtaa ctccatagcg atgctccttg
     2761 atttttttac ttgatacatt ccattttatt tctagataac acacgatcat atttcttgat
     2821 ttttccttgt aaacgttcca aacactcttg catttcagca atttctttcg tcaaaagttc
     2881 actttcttca atcaaaagct tacgccgctc aaatacagtt gcttctcctt cgctagatag
     2941 gcgaatatat tcaatcaaag actcgatcga taatccggca tttcgcatac agatcgtaaa
     3001 gtcgacccat tttagatctt cttctgtata atcacggatt ccgccttttg tccgattaac
     3061 tggtggaatc aacccgattc gttcataata gcgcaatgta tcggccgaaa tgcccttttc
     3121 ttcactgact tgtttgatat tcatctgatc actctttctc ttaatttctt gccactttac
     3181 ttatgtattt tatttattct acaactttaa gtgtactcca agtcaatact catttctatt
     3241 aaaactaagc tggattatct atatcggttg tttgtgtatt atttcaaata aaaaagtctt
     3301 gtttttttat tcttttttgg atatactttt aacaatttag ggaaatttta ggaaaagggg
     3361 tataaaaatg acaacgtatc agcgaaaggt attattatcg acatcggcgg gaattgcttt
     3421 agagaatatg gatattatgt ttttagcttt ttcattatct tcaatgatta cttcttttca
     3481 tattagtggt acgcaagcgg gtttcatttc aacgatcact aatttgggga tgctcattgg
     3541 aggtattttt tttggcatca tagctgataa atatggacgt gtgaaagtat tttctcaaac
     3601 ggtccttctg ttttcgattg cttctttatt gatgtatttt gcttcaaaca tctacttggt
     3661 ttatctcttt cgctttatcg caggtgtagg agcgggagga gaatacgggg cgtgtatgtc
     3721 cttgatttcc gaaagttttt caaaaaaaca aattggacga gcttcttcgg ttgctggtat
     3781 tggtgcgcag gtcggtgcgg ctttggctgc gattttagct gctgtgatca ttccaacgtt
     3841 tggttggaag atgttgtatg tcgtaggtgt actgccggtg ttgatggtct tgttcattcg
     3901 tcgcggcttg actgaaccaa aggaattcaa atctgtacaa tctgccgaaa agaaaaccaa
     3961 acttagcaga ctgtttgaat caaaacatct tgcttggcaa acatgtggcc ttagtatcat
     4021 ggtaactgtc caaattgccg gctattttgg gttaatgaat tggctgcctt caattatgca
     4081 aaatcgatta gggctttcgg tttctgggtc ttcattgtgg atggtcagta ctattttagg
     4141 catgtctttg gggatgatga cttttggcat aatcatggat aaattggggc ctaaactagc
     4201 cttttctttg tttttaattt gttcatcctt atctgtctat ctacttgtgt tagcccatag
     4261 ccagtggagt ttggtagcag ccgctgtgat agtcggctac tttatcaatg gcatgtatgg
     4321 tggatatggc gcagtgatta gtagtttgta tccaacagaa atccgcgcga cggcgaataa
     4381 tttcattatg aaccttggac gagctgttgg tggtttttct tctatcgtca tcggtttctt
     4441 gatggatcat tatagtttaa ctgccgtaat cttgtttttg agcggtattt acctagtcag
     4501 tctgctggtc ttgacaactc ttactggtgt tacagcatta agggaaagct tgcagcattg
     4561 atagttgagt ctgtaaatga caagaaagag gtgtggcaaa agcttgcatc tgcggtaata
     4621 agaccagcca aatcaaaaac atgagacact gtttccgttt ggctggtcgt attacttgat
     4681 gcagggcagc tttttcaact gctaatttcc ttctatctgg cggcgactat tctttgacgt
     4741 ccgtcatttc aaagtacttt actaataact tagcataacg aaaaactgtg gatacactcg
     4801 tatattgagc gtctgcataa tcttccaaat ttttctcagg atgttgatac atgaattcaa
     4861 tcaaatgata attatatgat tttctgtaga aatgaagata aataatgtag attgggaagt
     4921 tctcctgctt ttccatagaa aagcgccgat tattctcatt cttcaaaatg accttatcag
     4981 gatatttttg gaacaattct tctaaatctg ttagataatt caagatagac ttacttgata
     5041 ccttcaattc gttttgtagt tcttctattg aacaccaacg ttctgtttca agcagtaaat
     5101 gaatcatttt aattttcttt tgagaaactt ttgttaaaaa agtctgcata ttttctcctc
     5161 ctgtatctac tttactatac tacatacaag aaaaaaaacg gatacaaata tcatattgtc
     5221 acaccgtcaa tagtgcataa acctgttatt atttctcttt ttttcctaag tattctattg
     5281 atatgaatca tttaatcgtt tattatataa atgtcaataa tctaataata aaataacgta
     5341 catattttat tattgcatac ttaataacag aactatagca aaaaatcccg tgtatcaaca
     5401 cgggattaga ttaagtttcc tcaatcacta ttcataacac aattattata acaagtaagt
     5461 aaacaattga aaaggacatt tattgaaaac ggttgttttt tacttaagga ggaaatttag
     5521 cgaaaaaatt agttgcattg tttttggtcg caggaagttt gttgagtggg tgtggtgcta
     5581 ctacgtctac tactcaaagc acaagttcag atcaggcttc cagcagcagt accacgatct
     5641 ccgaaagtac ggcagattca tcgaaagaac aagaacaaat tgaaaagaaa aaagaagaga
     5701 aacaaaaagc ggaaaaagct gcactagaaa aaaagtgaca gaagcggaat ctgctttgtc
     5761 acaagctgaa gcaaatccta caaaagaaaa ctatgcaact gcttcagcag ctatccaagc
     5821 gctacctgaa aacaaacaag agttgacaag ccgtcttgct gcaattgatt ccacaatcaa
     5881 agcaaaagaa gcagaagaag aacggaaaaa gcaagaagaa attgcacgac aacaagcaga
     5941 acaagcgcaa caagaagcaa tcgcacagca acaagcagaa caggcgaggc aagaagcaat
     6001 cgcacagcaa caagcagaac aggcgcgaca gcaatcagca gctgaacagg cctctcagca
     6061 ggcacaagcc gcagctcctg agcaaaataa tgaacaaact gtctatgtca caccgaccgg
     6121 ttcaaaatac catacacata aatgtggaaa tggtacgtat acccctgcca ctttaagcga
     6181 agctcaagct cgtggtttaa ccccttgctc caaatgttat ggaaactaat tcaaaagtta
     6241 tactataata ataaaattct tctgaaacat aaaaaaatag aagatggcgt tccagatagt
     6301 aaaccaaaaa taacagttta cacgtccatt tcgtcagctt ctctcttttt tcatttagtc
     6361 tgttccctct gcttttttgg acatcttcat tcattctttc tcaatcaaat agtacacctc
     6421 tttttctttt cgcagtttat atcttccttt tttcaatttc attgaaaact gtttcgaatg
     6481 tttatgtttt acatggattt taagcaggag ctcctcatgt tgcaagcgat agttcctgcc
     6541 ttttaaatca gaaaatctca ctagtccgta ttcatcagaa gctttcacaa atggaacttt
     6601 ttctctagta atatagtgtt tgccgttgag cgtttttaca gaaaagacca ttccttcgat
     6661 tggtcgtccg tttgtgttct ttacataaaa agataaagga tagtttcgct tagcgatggc
     6721 caaagaatct aaataagcga tgagtagcca aactaagccg attgatccaa gtagtatacc
     6781 gataaaccaa ataaaaaatt tccatttttt ccattgatca attccttgga tcgctttttt
     6841 tgcctctttt gcttgataag gaatacgatg ccccgtcaca agcagtcgat gtgtattgac
     6901 catataagga gtacaggtaa gcaaagtcac gagatctctg cctttttgga tacccaaaga
     6961 atctacttca tcaggtaaga ccacttggat tttttctact tgatacgcca atgtctttcc
     7021 attgatctgg atgaaaaact ggtctccttt tttcaactta ggcaaatctg taaataattt
     7081 tgcttctggg agtccacgat gcccagaaag aacggcatgg gtgtttttcc cgccagtcgg
     7141 ataagaactt tcttccaata atgacgttcc tttttgcaaa aatatttccg ttgtctgatc
     7201 gaatataggt aagctgacat tgatttttgg gatttcgatg actccaagca tatgttccat
     7261 aaaaaaggca ttactaggta aatcttttaa tgcttttgca tcaactgctt ggttgaaact
     7321 gcctatccct ggtgatacgt tttgctctgc caactgttgg tttttctttg tcattttttc
     7381 ctgctgtttt tttatttcct ctgaattctt attcgatgct tgcttttgat aatggatgat
     7441 catttgctgg gcaagaaaat cattcagtga atcttctaca aaaggataag aaaagatccc
     7501 gatcccaacg actaaaatga ttgccatgaa gatgtccagt attcttattc tgcttttatt
     7561 catcatgtcc acctattttc taatatgtat atgaagaggt tgtgaaatca gcgccttgac
     7621 ctgagtatta gcgaagcaaa cggcaaaata gttcctccta ttttgccgtt tgttgagcga
     7681 ataccaagag accgaccact tgtcacaacc tttctcccca tttgaatcac tatcgtttat
     7741 ttatcgtatg atttttgttt ccaatagttt aagcttctgt ttcttgatgg cgttttttga
     7801 aatagatcac tgctcctcca actaaagcag tacctaccaa gatgaatacg atgatcccca
     7861 taccaccagt tgaaggcaag attcctttag gttggttatc gatcgttgtt tcatcagaag
     7921 ttgttggatc atcatctact gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt
     7981 agccttctgg tgcttttgtt tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg
     8041 aaaatttccc ttctgaatca gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt
     8101 catctgacca agcatatggg tattctcctg aggcaaggtt tgctattttt ttcattaatt
     8161 taacagttgg atcttttgct cttagaatag aaaattctgc atcggctaat ttttcttgcg
     8221 tgttcacatc ttgtttgatg aagtggaaat caccgtagtt ttggacattt tctacagtaa
     8281 tcgtgttatc cgtagttgta ttttcccgat tgactacacc gtttggattc ccaagattgg
     8341 cttcatctgc gccatggatg ataccggttg tttgttgcaa ttttccgttt tttactataa
     8401 aagtagtttc tttttgtgga ttggttaatt gataaccatc tggtgctttt gtttcaacta
     8461 aagtataatt gccgtctatt aatccattga cactgatgga tccttcagta tcagttgtga
     8521 atgtcaacgc tgactcagca ggatcataga gagcgacacc agtgttttca tcaaaaccgg
     8581 tcacgaattg attctctgaa tttttgatct ggaattgcgc ccctgctaac ttatctaccc
     8641 ctgaaaagtt cccagctttc tggattttga tattgctttc tttgatgaca tttttagcat
     8701 ataggtgaat cgtgtctaaa ggtttgtcag ggtctagggt aagcttaaca cgatatttgg
     8761 cgacgtttcg acaccgggct ttggtgtttc tacaaagacg tatacagcat ctttcccatc
     8821 agatttacct ggaagagtca ggttagctaa tccttcacca gaagtcgtta cacttgcgaa
     8881 gggtgtagcg tctgctggaa ctgctgtgtc tttacttgga tagtacgtag cagtgatgtc
     8941 atatacgttg aattcaacgc cgttcaacgg ttcgctattc ggaaatgtca attccgttcc
     9001 gttgtttatt tgattttctg gtgctgaagt gaatttcaat ttatgcaaat taaccgtaac
     9061 attttctggt gtaggtgata cggctgcaga aacctctgca gttcctaggc cgagaacgaa
     9121 aggaagcagc atgatagcag ttcctaaaaa cgtccatatt tttgttttga tgtttttcat
     9181 tttatttccc tcctattgtt tcctttttgt tgttttttat aaaaccaagc tgtccctgtt
     9241 gaaaggacta gcaataaccc taaaattgag aaacctccaa gcatctcttc gcttgttttt
     9301 ggaaagcttc ctccattttt ttcatttgat ggaccatttg tctgcatctg aactggcgtt
     9361 tttccattat tcgtcggatt tgcttttggt tcgttgatga tttccaatgg tataggatga
     9421 agcggatcat aactgccttt ttcaacttca aagagaatgg ttgattgact caatatatat
     9481 cctttaggcg gtttaatctc cactagttcg taacttccaa aagacaatcc atggacagaa
     9541 aatgttccat ctttactgga agtaaaaatc gttgctcttg aatcatcttt ggctacccaa
     9601 tgatttatcc cgttttcttg cgctaagtat tggtcatttt ttttgactag aaattgagca
     9661 tctgctagtt tttgtttcgt ttgccgatcc actttgacaa attgtttgcc tcctgtttgg
     9721 atggcaagat gtgtttctac tgtttgattg tcagtcgtca aacgtacatc gttatcaaat
     9781 gtcgtttttt cactgttttc tttcaatgtc atttggtaac ggatcgttaa tttttttcca
     9841 gcaaacgaac taagatcttt tccagaatct gataagacaa aaccatgttt tattttttgt
     9901 gtgtgaaatg tatgaatctt gtctccacca atcaaaaaat cgatcgtttc tggttctagc
     9961 cataactgag gatcagctgt atctttgatc gtataggttc ctatctcgtt gatgttctca
    10021 ggaattgtta cggtgatgag ataagggact ttatccccat ctgcaaaatc tgtctgctta
    10081 ttctctactg tttttgttaa tgtgagtgca gcaggtattt tttgttcgct ctttggatac
    10141 aagtggatgg tcgtcaattt atgattagat gaatctgtca caggaagcac aacgacaaat
    10201 ggtgctgatt tttcttttac ttaatctgaa gtctttattt ccgtaaatcg gtaaacagcg
    10261 tcacgacctt gctcatcttt gtccgatgca ctaaaagaag caatgccctc ttcctcgtct
    10321 actgtttttg tcacattttc tgccagtatt ttttcactat cacttttttg tgctagtttt
    10381 ctttgtgcgt cttccacact acttccttct gaccgcagct tgtaaaattc atccgagaca
    10441 tcatatatag cgaaagtaac gccgttcaac ccttttactc cttgaaagtt atccgggata
    10501 tttattccgt cgttttgact gatacttggc atcttatcgc tttcgaacat tcgattatgc
    10561 aaagtaaatg taataggtga ttctctagct gacacagtcg gagaacctat aaaaaagacc
    10621 agtaggataa aaaagagaac agcaaatata tccacacgta cattttttgt ttttttcatc
    10681 gctgttcacc tgcttttttc ttacgatgga taaaataaaa gaagccagaa aaaataaaga
    10741 tcagtcctaa aaagacaaag ggcagtagac ctatcccacc tgtcgaagga agcaagagag
    10801 tcggattgat cgtcaactta gcggtatctg ttgcgataat cgtataatta ttgtttacac
    10861 ttccttcgcc taaactaatc gttactattt gtgatccaat atctgtccgg ctgttaactg
    10921 ttagggtata atccaatgat tcgcctaaga caacaccggt aacgtggaga tttccaaccg
    10981 aaatcgagtg actttgggca tcataattaa aggcagcgtc atcgacagta atcactgcgt
    11041 tagctttata gatggtataa gttcctgatg tgttggctat atcagtaaat gacaacttgg
    11101 gatttgctgc tttgactttg tctattccgc tattcgttag tttaattgta tagctgccag
    11161 cattaaccat attttttgct gattgtgcat ctgctgtgta atccatactg ctaagtgcca
    11221 cttgtactgg agtaagcgaa ctagtgacac cctctgctag cttgacatgg attgacggga
    11281 cacctgcttt tccatcatat gtcttactgc cactgagctc taccgcaccg ctgatttgct
    11341 taatcgtata gacatcagat gtctgcacga tagtaaataa atagtttgtt ctggcagcta
    11401 gtttagctag tccgcttttt gtcaattgcc aagacgtata ggttccaatt tcagtaggcg
    11461 cagttacttc aactcccttt tcattcatcg ctttgaaatc agttggattc catgtgattt
    11521 gatcaatact gagatcattg tttaaaaggg atgttgattc tgttttatca ttaaaggcat
    11581 atttaacgat cggtacaaca gaaagaccat catagtactt actgccattt gcattgattg
    11641 atactgttgc atttgtctta taatatagga tgacttcaga aatctgtttt ccatttgcgt
    11701 cctttacgat tacatttcca tttgtatcta aactagctaa aacactcatt gctgattggt
    11761 tctttgtttc tatcgctgaa acagctgtaa aggtatatcc gttgatagta ggcgtattag
    11821 tcgtcaggtc ttgtgcagca cttccgtaag taagattcat tattttagaa ggagcaatca
    11881 tctgtccaaa atagtcttga tacttaatag tgattggtgt ttgtttgcct gtataggtca
    11941 ccgtatcgac gttactttct agggctgtga aagaaccgtt tgtttgtgca aaagaatagc
    12001 ccgttactgc tgttggagtg ataaccactt tgtcacccac atggattcca gataatgtag
    12061 tcccgtacgt tccgattttt gctgctccac cattgactgt ataagaaaaa gttcctgtcc
    12121 gattcgcatt gacctgaacc gttgctgctt gataggttgg aaaatttgaa acatttccct
    12181 taccatcaac gatctgccca tttttgatcg agtaccccgt gaatggaata tcatttgcta
    12241 actgtatacc gattgaaaaa gtactcatac ctcctccggc aggaagccct ccccatgttg
    12301 agggtgtaaa agaataactt ttatccgcat tgggtgtaac tgaatcgatc gtatttccag
    12361 cggtagccac ccccatatag atactttttg ggaatgtgat agtagtacca tttactgtgg
    12421 agccaataaa cagattatgg atatctggac ggttatcaat aaaagcgatt gctttggtta
    12481 cgccttgtaa cgcagtttta tccgcactca tcgatccgtt aaccgcaaca atgactccat
    12541 ctttggcata tatcactgta tatccatctg gtatggtatg tccgttgaaa ttcgcagtgg
    12601 ccgcaacagt actaaatgtc tggcttctac ctgtttctga ataagctttc tcccctgctg
    12661 cttttgctgc tgtgatttct ttttctgtcg gatcaatcag ttgagtcgag gatgtggttg
    12721 aggttatctc gttgttgcta gactgattgg tgctttctat ggactgtatt tcagaatagg
    12781 gtggactatt tgtctcctct gtcgtttggt tagcaaaatt tgacgtttct gtataaacgt
    12841 ttacataaat acttgataag ctccctaaga aagtaaaaat caagatgatc gaaatcataa
    12901 tgggatgtaa gtattttctt ttagatacag ctttgagtct agaaacaaaa tgtttttgac
    12961 atatacgttg gattttattt tgagttttct taatcatttt catccctcct ctcaataaaa
    13021 aatcatcaaa taatccttta cacgttattc taaatttaat tt
//
LOCUS       DAYYHB010000065        13053 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000065 DAYYHB010000000
VERSION     DAYYHB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.066"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACHA6B_001919"
     CDS             complement(<1..190)
                     /locus_tag="ACHA6B_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949484.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEA"
     gene            complement(207..1412)
                     /locus_tag="ACHA6B_001920"
     CDS             complement(207..1412)
                     /locus_tag="ACHA6B_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8949485.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2106..2492
                     /locus_tag="ACHA6B_001921"
     CDS             2106..2492
                     /locus_tag="ACHA6B_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8949486.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2586..2777
                     /locus_tag="ACHA6B_001922"
     CDS             2586..2777
                     /locus_tag="ACHA6B_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949487.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            3291..3623
                     /locus_tag="ACHA6B_001923"
     CDS             3291..3623
                     /locus_tag="ACHA6B_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8949488.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            3533..3727
                     /locus_tag="ACHA6B_001924"
     CDS             3533..3727
                     /locus_tag="ACHA6B_001924"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8949489.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            3766..3912
                     /locus_tag="ACHA6B_001925"
     CDS             3766..3912
                     /locus_tag="ACHA6B_001925"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949490.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            4350..5690
                     /locus_tag="ACHA6B_001926"
                     /pseudo
     CDS             4350..5690
                     /locus_tag="ACHA6B_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5931..7547)
                     /locus_tag="ACHA6B_001927"
     CDS             complement(5931..7547)
                     /locus_tag="ACHA6B_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8949491.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            7665..7883
                     /locus_tag="ACHA6B_001928"
     CDS             7665..7883
                     /locus_tag="ACHA6B_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949492.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8078..8539
                     /locus_tag="ACHA6B_001929"
     CDS             8078..8539
                     /locus_tag="ACHA6B_001929"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8949493.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8732..8971)
                     /locus_tag="ACHA6B_001930"
     CDS             complement(8732..8971)
                     /locus_tag="ACHA6B_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949494.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8995..10518)
                     /locus_tag="ACHA6B_001931"
     CDS             complement(8995..10518)
                     /locus_tag="ACHA6B_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8949495.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(10536..10658)
                     /locus_tag="ACHA6B_001932"
     CDS             complement(10536..10658)
                     /locus_tag="ACHA6B_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8949496.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(10688..11671)
                     /locus_tag="ACHA6B_001933"
     CDS             complement(10688..11671)
                     /locus_tag="ACHA6B_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8949497.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(11695..11844)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_001934"
     CDS             complement(11695..11844)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8949498.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(11865..12242)
                     /locus_tag="ACHA6B_001935"
     CDS             complement(11865..12242)
                     /locus_tag="ACHA6B_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949499.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(12274..12453)
                     /gene="rpmF"
                     /locus_tag="ACHA6B_001936"
     CDS             complement(12274..12453)
                     /gene="rpmF"
                     /locus_tag="ACHA6B_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8949500.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(12468..12737)
                     /gene="rpsN"
                     /locus_tag="ACHA6B_001937"
     CDS             complement(12468..12737)
                     /gene="rpsN"
                     /locus_tag="ACHA6B_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8949501.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         4214 a         2467 c         1975 g         4397 t
ORIGIN      
        1 tagcttcttc acataaactt tttactttat taattactac tttgtcatat tcatttaaca
       61 cactattatt accattactt agacgctcag attctacgaa acaactcaaa tcagaaattt
      121 cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta cgtaaatgat
      181 atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc cactaaacct
      241 aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga aaagtgtgaa
      301 gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga tttcaacatt
      361 attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat atgcatttca
      421 ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc tatttccttt
      481 acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga aattaaggga
      541 gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat cccagaatcc
      601 ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat caaagcatct
      661 ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg tgctaacttt
      721 tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact aacacttgga
      781 attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc tccattttta
      841 acatattcca gtattttatt ttcataaaga aacagatata tttctggatc gtaattttgt
      901 gataacagat tatctatttt gatttgctcg atagttttac ttagattttc tgtatattca
      961 attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg aactaaaatc
     1021 agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga attatagagt
     1081 cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa aaaaacttta
     1141 tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaaa catgtataga
     1201 agaaacatct cattaaaatc accagtaaag aaatggaaat ctttagtttt tttaatatta
     1261 tcagttagta actttttata atcataaaaa aaataagtag ttctatctga gcgatactct
     1321 tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga
     1381 gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc
     1441 attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt
     1501 acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt
     1561 ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc
     1621 aaagacaata attattatga tccgttatta tctaagcact atacagataa taggaatttt
     1681 tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta
     1741 ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata
     1801 cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc
     1861 tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc
     1921 ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac
     1981 atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg
     2041 cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt
     2101 gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg
     2161 tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac
     2221 aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga
     2281 ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt
     2341 tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata
     2401 aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac
     2461 tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc
     2521 ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg gaagaaggta
     2581 gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat gaagaagctg
     2641 ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg agaactggaa
     2701 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
     2761 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
     2821 aatttcgaac aataaatttg aaactatctt ctttataatt ataaagtatc gattttacag
     2881 cgttctataa tgattcatag aatctaaaac gaatgatttt aattatcaac gttttttaat
     2941 tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta ctttatttac
     3001 gtatgaagtg ggcaggaggt cgaatccaga actgggctat ggtacttcaa cgaggacttc
     3061 tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat ttattcaact
     3121 atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt ggttgttaaa
     3181 aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa gttgggctat
     3241 gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta ttgagaaaaa
     3301 catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa aaagtaacta
     3361 ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga tgggtttcag
     3421 aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta cctgataaag
     3481 cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac gagtgttcta
     3541 cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg atgaaggaat
     3601 cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat gcacaagagg
     3661 atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa tggtaaaaaa
     3721 atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgat ggatattact
     3781 tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt agcaactcgt
     3841 attctacgtt actatatggt agattcacac atgaaaaaag agattgtcgt ccgtccatta
     3901 caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag ccagcatcgg
     3961 tcttttattt atcaagagct gttgaacgac caccacatca cacattcagt gagccaaact
     4021 gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa atgagaatta
     4081 atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca agattattta
     4141 gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc ttgacaattt
     4201 gaagcgaaga aaataaggga tctataatat ctagtgttgg tgacgccttc tgttgccaat
     4261 actttttctt ttttctaata aacgcaaaca gacaccttaa aatcctttag aataaagtcg
     4321 acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc aaagagttac
     4381 tagatattct agacctaaat cttatctttc atgaagatgc ctttcgcaaa gagcgaatca
     4441 atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccggag gaatgctttc
     4501 atcgccacta tcaaaatgaa caaacgatca ttaaataggg ctggaaaaaa gtatccatct
     4561 tattgaatga tgtcagtaac tacaagacca ttctgcggat taacaaacag cgcttcaaat
     4621 gtaaacactg tggaaagact tttttagcag aagattcagt tagtgatcgt cgctgctcca
     4681 ttgctcgcag agtcaaacaa gccattcttg agctattaag cgaacctctt tctatgtctt
     4741 tgatcgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt cgcagccttc
     4801 ggcctaaaac catttctttg agccagctgt taccagaagt ggtgtgtttc gatgaattca
     4861 aatcagtgaa aaatgtctcg ggagccatga gctttgtcat gatggacggc aagacccatc
     4921 aactgattga tattgtggaa aatcggcaac tcaatccttt acgcgactat ttcttgcgct
     4981 accctataaa agtccgtgaa caagtgcgtc tagtggtttc agatttctat acaccctatc
     5041 gcacattggt gaaagagtgt tttccgaatg cccgaatcgt cgccgatcgc tttcatatta
     5101 gccaacacat tggtcgggct tttaccaacc accgaattca agtaatgaag acctttaaaa
     5161 aaggggatcg tcggtcgaag cacttaaaga aatactggaa actacttcaa aagaatgcgt
     5221 gggaattgaa aggtcagcat cgctactggc gtccgtcatt tcgagatcat ctgacagaag
     5281 cagagatagt cgatcggcta ttatcttatg atgattcctt aaagcgaggc tacgaagtct
     5341 accaggattt tctttccgtt atcagaagac aagatgttcc tgaattcgtc gcgctcctaa
     5401 aagaagacta caaggagttg ccagagcact accaaccagt gttcacgacc ttcaagaaat
     5461 accaaacaga aatcaaacga gcgttgcgcg ttccctattc caacggccct atagaatgtt
     5521 tgaataacca tatcaaagta ctaaaacgaa ttgcctatgg ctatagaaac tttcaaaact
     5581 accgagaacg aatcttttta tatcgaggaa aatattttaa gaaaactaag aatactaccc
     5641 aactgactaa agccagaaca acaacacggc tagacaagat agctgtctag ccgtgtgagt
     5701 atctcgatta ttctattgaa tttcttcacc aacactcatt gacgaagagc caaaataagt
     5761 aatctaaccg actgttaact aaaccattgc aggtcccgtt aaagatactt gtgcctgttg
     5821 tcaccgcttc tttttcttgt ccacaagcgc ttaacacact taataaagcc cctgctatta
     5881 ttaataataa tatctttttc ttattaaaaa cctcctcatc tatttccaga ttagtaaatg
     5941 tagtaatctg tgctttcttt tttctcaata ctaataacta acttatgggg taaacaaata
     6001 ctcgtttgac cgggttttga gatccatcca gttctaaccg cgatttgatc aggactatta
     6061 tcttctttaa ctcgaattct tccatcttta atctcaacaa tattatattg tcccttcgca
     6121 ggatgataat tcacgagttt atgattgatc ttatccaaat cgaagcgatc catttccttg
     6181 ccatcaattc gaacgatggc atatttgata gcatcttcag ttgtttcatt ctccttttct
     6241 atagagaata caaataaggg aataaaagag gtaataatca aaatgccaat aattatgaca
     6301 tcgcctatct tgacaccagc ttttcttaaa aatttcatta acgatatctg gctaaacata
     6361 ttagactttt tccatggaaa tggtgaaatt tttcggaaat tatctgcatt aagagaatca
     6421 gttaaacaac cctcaagttg ttccttaata cattgttgat ccatcttcat accaataaaa
     6481 actagtttat taatgcgttc accaaacaac ggatgccagg cttccttcaa ttcgggattt
     6541 ttttgtataa tcatttgaca ctcaatttta ggtaaactag ctacccaata atttaatgat
     6601 tcaatacttt tatagctacc cgatgtttca aagatcgtcg aaaaatcact atgttgggca
     6661 aaccaaagaa acccttttgc tcttgtaata ttgccaggaa aatttttatc cgtaagatgg
     6721 gcaaattttt gtgcgtcaag gagtaaccga tttcgataga taaaacttga gataccatat
     6781 tcttctgttt caggaatatg attttgatag ccaagctcta actctttgaa ccagcccact
     6841 gaataggaag tctcatctat attaaaaaga tttgtattta gaattttcga taagtctact
     6901 tcacctttaa ttgtcctaat tattttggca ttggactgta acttagcaat tagaccgtta
     6961 attttgtaaa tcgtcttggt tgacactaag tcactattat ttaaaagtaa tatgttacaa
     7021 cactcgaatt gatcaattaa caagccacga atatcctgtt cttcttcaac tacattacct
     7081 tcagcttttc tttcctgaat cttttcaccc gtttcgaaat tatcccaaaa acgttttaca
     7141 tccactacta taataatggc accaatctct aatcgttggg tcaaatcaat gtctaaaggg
     7201 tttttagcta atataattgc ttgaataatt ggaattggtt tgctgattcc tgaagcttca
     7261 ataacaattt gattcaagtc agaaaattgc actaattgat ctagttcaac aatcaaatct
     7321 tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa caagtttttc tccagttctt
     7381 ttaaaacctt tcttgtcgac taatacccca tcaacattta cttcccctat gtcatttaca
     7441 atgacagcaa tttcctttcc gtgatcaccg tacagaatat gatttagaag tatcgttttt
     7501 cctgaaccaa gatagccagt taagattgta ataggtattt ttttcatagg gaacctcttt
     7561 ctataccttg aatttacact ttaagtgcaa cacataaaaa tgagtcataa tcgatatcca
     7621 actagtataa gtttgtcgag aacactccta gaaggaaaat aattatgagc cagtatcgtc
     7681 atcttactat actcgaacga gaaagaatct ttctgcttca cgaagaagga ttttctatta
     7741 gtataattgg caacgacatt cagcatagcc cgtttactat tttacatgaa ctgagaagaa
     7801 acaagaaaaa aactattcac cttccaaagc ggaaaaaaat tatgcttatc gaagaaaaaa
     7861 atgcggacgt tatcacattc taaatgatcc gtgagtttgg tcaattgttc gtcatttatt
     7921 tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt ttgaagcatg aacatatgga
     7981 cttttcaatt agttttatca cgatctaacg tgcaatttgc aatgatcttt tcgatacaga
     8041 acccctctca cacggcaata aaggcctgat ccgttcattg caccatcgag gaaaaacaag
     8101 acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc cttactcatt cgatccatgg
     8161 ccgtccaaaa tccgcagatg atagaagtga ttttggtcat tggaaagccg atattgtagc
     8221 tggcaaaact ggtggtactt gtttagtgac tttgacagac agaaaatcat gcttcctttt
     8281 agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg agcgagaaaa tgattgaact
     8341 tttaagcagt attccaaatg aggcggtcaa aacgatcact ccagatagag gaaaagaatt
     8401 ctccaatcat ttcgcagtta cagaagcatt gaataatatg cccttttatt ttccagatcc
     8461 tcatgccctt ggcaacaatg aacaaattaa aatacgaata gactcattcg agaatacttg
     8521 ccaaaatcga aagagatagc tacggtagat aactcaacga taaatcagat gattgaaaaa
     8581 ctgaataaca gacctagaaa gtgtttagat tggagaacac cttatgaagt cttctttgat
     8641 aaagtgttgc gcttgatttg acaattcaag acataataaa aagcagatca aatccaaata
     8701 agggaaaaat gatctactct ggtattatca attaattggc caagctatta tgccgttttg
     8761 ctaaacgtct acttcttcca gtattgacct tgataaaaca acaatcagga tcccgtttta
     8821 atactctatg aactgtgtca atactaacat gtaaagttgc agcaatatca gctaatgtac
     8881 tcccatttaa tctcatttgc tttgcaaatc gataacgccg ataagcatca atattcttgg
     8941 aatccataga caataatact tttcttacca tgtatttcct ccaaaaattg aattttaatg
     9001 agccatcatt tcttgagcaa tttcatgttt agacaatgaa gctggataga aagtaggcca
     9061 attatgcaat tcattataga gtttttcttg actttctcca ccaaagaaaa tatggaaatg
     9121 ctctgcctta cttggtgcaa ttccatgatc actaaattga atatatttaa aacgaccagc
     9181 acctgcatca tcagtttcaa aattaaaacg aacaccacgg tttccttttt cataattcaa
     9241 aattttgtat ccaacatatt tgtatgtgta ttgatgatgt tcaccgttga ctacaaaatc
     9301 aatggtgttg tctttaatgt tgatagtatc aatatctgtc ttatagccag tagtataata
     9361 atctttgtac tcggcagctg tcatgtcttt attgattttt gctttgtaat cgaaaacttc
     9421 atccaaaatt ccatctttta acaacggata aacagattgc cattcgcctg tataatcact
     9481 caattcgcga tccttcacat cggcatcttt aaagtaccca ttagcaactg tcttttcatc
     9541 cttcgcttct ttttctggaa ctacctcttc accagcaacc atagttgttt tttctaacgc
     9601 ttttagattg tcttccatta ctgaaacata atcttcacca ttatccattt gttcttttgt
     9661 aagactttca agaggattca gtacttccaa ttttatccca gcttcgttgg ccaaagtttt
     9721 tgcaatttta tcattagcat tcttttcaaa gtaaatataa ttgatcttat tttttttaac
     9781 atactctttt aattcagcta atcgaccaga agatggctct tcttcaggac ttagtccagc
     9841 aataggaact tgtttcaatc cataatccaa agctaaatat ccaaaggctg cgtgttgcgt
     9901 cacaaaactt ttttgtttcg cttctttaag actagtggta tagtcagcat ccaaccgttt
     9961 cagtttagtc aaatactttt cagcatttgt ctcaaatact tttgcttttt tcggatacaa
    10021 tttaactaac tgatccttga tatttgaaac ttccttaatt gccattttgg gtgaaaccca
    10081 cgtatgagga tctagttcat gatggtgatc ttcttctccg tgatcatgat cttcttcttc
    10141 actacctggc aataaaatca tgtcttttgt tccttctact acattaggcc cagctttctt
    10201 ccaactttct ttagcttttg gtacccatga ttccatgttt tcgttatgat atacaaaaac
    10261 atcagcatca tgagcttttg ccatatcctt agctgagggc tcatagtcat gaggttctga
    10321 tcccgctgga accattaaat ccacgttcgc ttcatcgccg acgatgtttt tggtgaaatc
    10381 atacattgga tagaatgttg ttacaatttc taattttcca tctttatcat tcgatacttt
    10441 tgcatcatta gtgctggagc aagctcccaa taatcctaga gctgctatcc caataagtcc
    10501 tacactgatt ttcttcattc tttttccttc cttaattatg ctaatttctt cattttattt
    10561 cgtttatgtt ctttgataat ttttaatgcc ctttttctag ttttaatatt cgggctttta
    10621 agtcgtcgat atgcactaga tatatccgtt ttctccatta gaaattaccc ccaatcacta
    10681 cttatcatta tttgctttcc gccagtaatt tatcaaaact ctgtttgatc ctttcttcat
    10741 ccaacatttt tccgattaga ataatagtgg tttggtcttt ttgtcgagtg gtgtattgaa
    10801 aattaacctg ttcgtttacc tcttgcatgg caactataaa atcctgctct tgtaaagcaa
    10861 tcaatccttt aaaacgatac aggctccctt ggttggttaa aatcagccag tcaatccatt
    10921 ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa gagaattgat ttaaactcat
    10981 gtacttgatg gtgttgatga tagtatttgc cattatagtg attttctgat gaatcacttt
    11041 caaaaactaa gggatgatta aattttttca aattataaaa atcctttttt tgaatcgttt
    11101 cactatgtgt gaacattcga atatcggata aagggttaat acttttgatt tctctctgta
    11161 atttttttag tgcagataaa ttcaagtttt gttttctaga cacaaaaatg cgatctgcca
    11221 tgacaagttg cttaagggct tgtggttctc tttcaataag ttcaaaactg tcactatcaa
    11281 taacagtcaa taaactatcg atatatactc ttcctcttag ttgaggcgct gttaaaagtg
    11341 tttgaatgat tggttgggga tctgctatac ctgtagtttc aatgatgatt tgtgaaattg
    11401 gttgttgatg ttcttctatt gtttccagta aagaaatcaa tattgctaat aaatcactgc
    11461 gaagactgca acacatacat ccattattta gttgtaacac ccgttcctcg ctatgaacta
    11521 ataatttgtg atcaatccca acttcaccaa actcattttc aattataata atttcttccc
    11581 tggggtaggg tgagacttgc aaggcttgat tgattagcgt tgtttttcca gcccctaaaa
    11641 acccagaaac gattgtaact ggtatgccca ttttgtcacc tttttaccac ttagctattt
    11701 cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa tactttttta attctaaccg
    11761 ttcagggtta ttccgtttat ttttgctggt gagatacaga cgttctccag tttcgacaca
    11821 ttccaaaata atattttgac gcataaatct tcctccttca atacttatcg atcttcaaga
    11881 tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc cagattccaa ttgtgaaact
    11941 aaattttctt tcatatcaac aggtatctcc caataaccat cgatacattg aaataactcc
    12001 tcgtcatact cttcaaaaat tggatagatt cttaaaaaat catcaaatga taagccttgg
    12061 tcctcatagt aatctttgac taatccattt tcaattgcct gttgggtcgg taaacccgtt
    12121 tcagtaataa ttcctctctc taccatcgaa cgataaaaag tgcatttatc tagcgattgc
    12181 tgggtcgttt caagataata ctttaatcct tcttcaacta aaggtgggta aataatttcc
    12241 attttgattc cctcctaaaa gttatatatt aaactatttt gaagctcctt taattacttt
    12301 ctttccatta tagtaccctt ttaaagatac atgatggcta cgtctataat caccattcgc
    12361 ttcgtcaaat gaaatttctg gttttactac agtttgatga gttcttctta accgtttctt
    12421 cgccttagat gtttttcttg ctggtactgc catgatattt cctcctttta ccaacttgct
    12481 ttttttactc caggaatcaa cccttgatgg gcaagttctc taaacttaat acgggacata
    12541 ccgaactttc gcatataccc tcttggacga ccatccgtct gatcacgaag cctcagacga
    12601 ttcggatttg aatctcttgg caatttagcg agagcggtgc gatctcctgc ggcttttaat
    12661 tcctgacgtt tatctgcata cttttcaatc atcgccattt gtttctttgc tttagcaatt
    12721 ttcgactttt ttgccatata cctttatact ccttttattt tgatttagac acctcaaatc
    12781 gtaatcgtta cgttttgaca ctgatacttt accaacaaga gttgctcttg tcaacaataa
    12841 ataacatgaa ataaaactca tatgaataga taactatttg aaaacgaatt tatcggtaca
    12901 tagagataat caaatcattt aaaagaatat gcagtttagg ctctgaaaaa taagaattca
    12961 cttttattag tccaatcaaa cacaaatacc gttttaagta gcttttcctc tacttaaaac
    13021 ggcaatatga aagaaactat aactttttat tca
//
LOCUS       DAYYHB010000066        13024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000066 DAYYHB010000000
VERSION     DAYYHB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.067"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..501
                     /locus_tag="ACHA6B_001938"
     CDS             28..501
                     /locus_tag="ACHA6B_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8949502.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            510..737
                     /locus_tag="ACHA6B_001939"
     CDS             510..737
                     /locus_tag="ACHA6B_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949503.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1373..1744
                     /locus_tag="ACHA6B_001940"
     CDS             1373..1744
                     /locus_tag="ACHA6B_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949504.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2000..2242)
                     /locus_tag="ACHA6B_001941"
     CDS             complement(2000..2242)
                     /locus_tag="ACHA6B_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8949505.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2274..3176)
                     /locus_tag="ACHA6B_001942"
     CDS             complement(2274..3176)
                     /locus_tag="ACHA6B_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8949506.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3189..3377)
                     /locus_tag="ACHA6B_001943"
     CDS             complement(3189..3377)
                     /locus_tag="ACHA6B_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8949507.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3391..3954)
                     /gene="amaP"
                     /locus_tag="ACHA6B_001944"
     CDS             complement(3391..3954)
                     /gene="amaP"
                     /locus_tag="ACHA6B_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8949508.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3992..4885)
                     /locus_tag="ACHA6B_001945"
     CDS             complement(3992..4885)
                     /locus_tag="ACHA6B_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8949509.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4963..5886)
                     /locus_tag="ACHA6B_001946"
     CDS             complement(4963..5886)
                     /locus_tag="ACHA6B_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8949510.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5935..6285)
                     /locus_tag="ACHA6B_001947"
     CDS             complement(5935..6285)
                     /locus_tag="ACHA6B_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8949511.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6318..7220)
                     /locus_tag="ACHA6B_001948"
     CDS             complement(6318..7220)
                     /locus_tag="ACHA6B_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8949512.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7213..8070)
                     /locus_tag="ACHA6B_001949"
     CDS             complement(7213..8070)
                     /locus_tag="ACHA6B_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8949513.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8404..9213
                     /locus_tag="ACHA6B_001950"
     CDS             8404..9213
                     /locus_tag="ACHA6B_001950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8949514.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9253..9750
                     /locus_tag="ACHA6B_001951"
     CDS             9253..9750
                     /locus_tag="ACHA6B_001951"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8949515.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10135..10208)
                     /locus_tag="ACHA6B_001952"
     tRNA            complement(10135..10208)
                     /locus_tag="ACHA6B_001952"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10172..10174),aa:Ile,seq:cat)
     gene            10395..10718
                     /locus_tag="ACHA6B_001953"
     CDS             10395..10718
                     /locus_tag="ACHA6B_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949516.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10882..11136
                     /locus_tag="ACHA6B_001954"
     CDS             10882..11136
                     /locus_tag="ACHA6B_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949517.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11206..11451
                     /locus_tag="ACHA6B_001955"
     CDS             11206..11451
                     /locus_tag="ACHA6B_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8949518.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11527..11892
                     /locus_tag="ACHA6B_001956"
     CDS             11527..11892
                     /locus_tag="ACHA6B_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8949519.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11953..12516)
                     /locus_tag="ACHA6B_001957"
     CDS             complement(11953..12516)
                     /locus_tag="ACHA6B_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949520.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            12494..12661
                     /locus_tag="ACHA6B_001958"
     CDS             12494..12661
                     /locus_tag="ACHA6B_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949521.1"
                     /translation="MPFLIYNTVIFKNKNAKSLYNNGESTKNKKIITLLKKQMKDQVL
                     LDLILSSLEGI"
     gene            complement(12787..12909)
                     /locus_tag="ACHA6B_001959"
     CDS             complement(12787..12909)
                     /locus_tag="ACHA6B_001959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949522.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT         3782 a         2430 c         2194 g         4618 t
ORIGIN      
        1 gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc
       61 gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct
      121 acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac
      181 tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc
      241 gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc
      301 attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat
      361 ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca
      421 gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca
      481 gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat
      541 tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa
      601 acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa
      661 tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata
      721 ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat
      781 agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt atttcgattg
      841 aataccgttt tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc
      901 tccatgtact atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg
      961 cttttagtat aatactttat tcattccacc agactgatag cagtttgagc agtctaaata
     1021 atcatgtagt attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa
     1081 tggcccattg tgttaactct tactccattc ttttttagta agctcccata tatactgtaa
     1141 agccttctat atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat
     1201 gatttgaata acagtgatgg acaattcttt tttattttta acattatttt gataataatg
     1261 attatttatt taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac
     1321 aaatatcgtt attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt
     1381 tattgtttac ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct
     1441 gctcgtcaat cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt
     1501 acttctattt gtttgtctag attactttac gagctattat tggctaaaac agacaagagt
     1561 aaaaaagaga tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg
     1621 ctttttactt catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt
     1681 cgtattattt tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta
     1741 ttaaagaagc ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa
     1801 ttgggaattt gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt
     1861 ccgtataaac agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat
     1921 atgagaagct atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga
     1981 cctcttttta aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa
     2041 tgatggcacc aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac
     2101 ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc
     2161 ccatcgaaga acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga
     2221 taagtgacca aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga
     2281 acccgtggtt ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact
     2341 ttatctgttt gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct
     2401 tgcaccgttt cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg
     2461 ttgacttcga tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc
     2521 atttcttcaa agattttttc gatatctttg ccatattcca caatgacatc catatcaact
     2581 gctacttgtt ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca
     2641 agttttcctg ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt
     2701 tcaattgcga ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc
     2761 attgaatctt cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt
     2821 tttccttgtc cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa
     2881 ggatgttttt gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact
     2941 ttagattctg gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct
     3001 tctactcctt gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt
     3061 ttgttctttt catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca
     3121 gacactggtt tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat
     3181 gtttcctcct aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg
     3241 atagcgacgc cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg
     3301 atccataaaa cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc
     3361 gaaaatagtt ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg
     3421 tttagctggt tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc
     3481 caatatgtgt tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag
     3541 tgctgaagtt cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt
     3601 gacatttact tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc
     3661 ttcaattgct tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc
     3721 gattgttttt ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc
     3781 aacggcaagc caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa
     3841 cttctctagc tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac
     3901 agatagcaaa agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc
     3961 acctcctcat aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta
     4021 ctccgtaaat ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg
     4081 gtgctaattc tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga
     4141 tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta
     4201 caccatttac acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag
     4261 cgattgaagc ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa
     4321 cagaagttgt tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta
     4381 aagcaaacat agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact
     4441 cagcaataga ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc
     4501 caagttcact cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa
     4561 aaggtagtaa gacaatctta cgtcctgcat ctttcacgta ttttgctact tcctctgcat
     4621 ctttttcttc tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa
     4681 tcgctgctgc acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta
     4741 gttgattatt gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct
     4801 gtaaagctgg tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg
     4861 ggtcgattaa ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc
     4921 aaaacaaatt tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt
     4981 ccggtataaa tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat
     5041 aaagcctaga gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc
     5101 gaacaacgcc ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt
     5161 tagattattg tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata
     5221 gatctcgatc attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc
     5281 atcgtctaaa cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg
     5341 atctcttaag gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag
     5401 ttcttcattt tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat
     5461 ctctcttagt tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt
     5521 ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt
     5581 tctatcgata caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt
     5641 tttttcgata cgattatctg actgatctag attaatcact accaaagaaa atggatgctc
     5701 tagtttcgtc tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac
     5761 gaaaatatca tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc
     5821 actatcaagt acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc
     5881 atccacaaag tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct
     5941 gaagaagcag ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga
     6001 aaagctgtca agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc
     6061 taatttagat gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa
     6121 ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct
     6181 tagaaatgga tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt
     6241 ttcaattaac gtctctactg catttttaga cattgtaatt tccattccta ctccccctct
     6301 tcagttgttg tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat
     6361 tttggatatt tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg
     6421 taatatttct tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa
     6481 tggataatgt aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga
     6541 tgtttttttg cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac
     6601 caacggtcat gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga
     6661 ttatgttcga tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca
     6721 ttgatcagca tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt
     6781 tccagtcgtt gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca
     6841 agacgccata atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata
     6901 cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct
     6961 ggaatacgat cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc
     7021 acatctgagt tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa
     7081 tcgatatcat cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat
     7141 actgagacaa ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact
     7201 gctatttctt ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt
     7261 ttttaagtat aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc
     7321 tgtaaagtga acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc
     7381 atttggtgct ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag
     7441 ttgttcccaa tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg
     7501 atcgccattt tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg
     7561 atcaatgtca atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa
     7621 tctttccaaa gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa
     7681 cggctcatca tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag
     7741 gactttctta taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt
     7801 agttgtgata tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa
     7861 ttggatactt gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata
     7921 ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc
     7981 taatgcagtt gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac
     8041 cacaggaact acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct
     8101 aacctgttct acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt
     8161 ttcattgaat attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg
     8221 tttttcatgg ctttttattc aaaggtattg accaaaaact actatatata gccacctttt
     8281 tcttaagaat gttttttgaa aaaattcata atgacaaagc aagttagttt cattttttta
     8341 aattctcatt taatcttaag gtaacaggaa aatagatatg taaaaagaaa gtaggaagat
     8401 aaaatgaaac tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa tggaaagata
     8461 ccagaagaaa atatccaagc acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc
     8521 gctaccggaa ggaacagcat ttctgctaaa gatatttttt ccgaattagg aatcggtggc
     8581 tatttgattt cttccaatgg ttcactgatt gctgaaatga aggatggaaa aatggcgaaa
     8641 gttctcagac gttcaaaaat agatagcgat acactgagaa aagccttctt ctatgctcaa
     8701 gaagcgaaaa tatccatcat tgctagccga gaaacccaag atgatcagct gacattcgat
     8761 gaatcaaatc tagtcaaaga tgacccttat tatgaacatt tcaatttaca gaaccattct
     8821 tttgatcaaa ttttaaatat gttggaagat ccttctctta gctatcttaa attagcatta
     8881 acagataaga gtgaagaaaa actacagaag ctacaaagag atctgcaagc agatggtatc
     8941 caatcagtat tctctgatcc gcatttctta gaaatcacgc ctacaggtat cactaaagct
     9001 cattcacttg tttttctggc ggattacctt ggaatcgaat cgagtgacat catggctttt
     9061 ggcgatcaag aaaacgacat tgctatgttg aattacagcg ggttgagtat tgcgatgggg
     9121 aatgcacagc gacaagtaaa agaaattgca aaccaagtaa cactgacgaa tgaagaagct
     9181 ggtgttgctt atattttaaa aaacttctat taaaaagaat aaaatgacga tatgaatgta
     9241 ggaggaaaga aaatgttagt tattattgat atgcaaaatc atattcttga tccaaacagc
     9301 gaattttata ttccagagtc agatcaattg gcagaacgta ttgctcaaag attagctaaa
     9361 gcacgccaaa ataatgaata tattctcttc acacgggata taccagttga actgaaggac
     9421 aaagaagaag aacgagaaga tttgcaattg atccctcttc tttctccaca agaaaatgaa
     9481 cgagtgatta aaaagtatta ctttacgatt ccacctgaaa ctttagctga aatccaggaa
     9541 actttatttg aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac
     9601 ttgtgtgtac tctctaatct gataggcctg cagagtgctt ttcctgaagc agactttttt
     9661 gttgatccaa tgttagtctc cagtagaaaa cacggagaat ccgctttaga gctgttgaaa
     9721 gatttcaacg tctctatcgt cgaatcataa aaatatctat cttcatttac gatatttaac
     9781 acacaataaa aaaagcacgc agaactgttt tagtcctacg tgctttttct atcaaagtag
     9841 attggctttt ttatgacaca gagtgaaacg gcaggctcac gacctctttc ttaaatggtg
     9901 ttcaatcagc tgaccctcgg tattagctta acaaacggca aaatatgagg aactattttt
     9961 ccgtttgtta agctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg
    10021 tattcaaaag cttgcatctg cagtaataag aacagccaag taaaaacata agaagctgtt
    10081 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttatgtacc
    10141 ttgtaggact cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct
    10201 aaaggtacat cataaataaa atttattttt gttacttttc aactatagca cgaatgacaa
    10261 actctcacaa gtttcaaaat ataagtaaaa taatattgtt attcttattt cttttacttg
    10321 tgtcataact attttgttac tttaattttt atttgcttat tttagaaaaa atgttattct
    10381 tttatttatt ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt
    10441 tagttgcggc agcagcttat tttctccaat taaattatca aacctcagga attctgttta
    10501 ttggaatgat ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata
    10561 aaggaaaaag atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct
    10621 atgggatcat cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga
    10681 attacttaga tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca
    10741 gttcctgctt atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct
    10801 tttattttcc tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg
    10861 aatcgttgag gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg
    10921 gtcgcactgg tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta
    10981 gctattttga ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt
    11041 gaacccgata gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc
    11101 attctttatt tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct
    11161 ttctatagtc attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa
    11221 gctattttac tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta
    11281 tctcaagtag ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa
    11341 gaaggagaag aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt
    11401 tccgttgatt acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca
    11461 gccttggtgc tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac
    11521 atttctatgc gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta
    11581 ggacaacatc gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact
    11641 gtggattatg tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg
    11701 gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt
    11761 ggcaaatcat gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat
    11821 ccggaacacg atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt
    11881 tatcttacct aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt
    11941 aaccatctta gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc
    12001 aaaactaaaa gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca
    12061 tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag
    12121 attgcttctt ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt
    12181 actacatcga tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc
    12241 tctgtagtca ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt
    12301 tgtgatcagt tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat
    12361 tgatagtaaa aatagttttt ttatcataag gttccccccc tgaaaataga ctaaaataat
    12421 aacgctttct ttttttgttt tcagacgaaa attaataatt tctaaatatt ctttaaaaaa
    12481 gcttataact attatgcctt ttttaatata taacacagtt atttttaaaa ataagaatgc
    12541 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
    12601 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
    12661 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
    12721 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
    12781 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
    12841 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
    12901 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
    12961 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
    13021 tgaa
//
LOCUS       DAYYHB010000067        12803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000067 DAYYHB010000000
VERSION     DAYYHB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.068"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..475)
                     /locus_tag="ACHA6B_001960"
     CDS             complement(<1..475)
                     /locus_tag="ACHA6B_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8949523.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAII"
     gene            complement(472..1125)
                     /locus_tag="ACHA6B_001961"
     CDS             complement(472..1125)
                     /locus_tag="ACHA6B_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8949524.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1413..1933
                     /locus_tag="ACHA6B_001962"
                     /pseudo
     CDS             1413..1933
                     /locus_tag="ACHA6B_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1931..2650)
                     /locus_tag="ACHA6B_001963"
                     /pseudo
     CDS             complement(1931..2650)
                     /locus_tag="ACHA6B_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2601..2810)
                     /locus_tag="ACHA6B_001964"
     CDS             complement(2601..2810)
                     /locus_tag="ACHA6B_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949525.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2792..2860)
                     /locus_tag="ACHA6B_001965"
                     /pseudo
     CDS             complement(2792..2860)
                     /locus_tag="ACHA6B_001965"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3087..4360
                     /locus_tag="ACHA6B_001966"
                     /pseudo
     CDS             3087..4360
                     /locus_tag="ACHA6B_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4467..5498)
                     /locus_tag="ACHA6B_001967"
     CDS             complement(4467..5498)
                     /locus_tag="ACHA6B_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949526.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5505..6335)
                     /locus_tag="ACHA6B_001968"
     CDS             complement(5505..6335)
                     /locus_tag="ACHA6B_001968"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949527.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6332..7147)
                     /locus_tag="ACHA6B_001969"
     CDS             complement(6332..7147)
                     /locus_tag="ACHA6B_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949528.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7144..7968)
                     /locus_tag="ACHA6B_001970"
     CDS             complement(7144..7968)
                     /locus_tag="ACHA6B_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8949529.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7958..9058)
                     /locus_tag="ACHA6B_001971"
     CDS             complement(7958..9058)
                     /locus_tag="ACHA6B_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8949530.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9236..10021
                     /locus_tag="ACHA6B_001972"
     CDS             9236..10021
                     /locus_tag="ACHA6B_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8949531.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10054..10437
                     /locus_tag="ACHA6B_001973"
     CDS             10054..10437
                     /locus_tag="ACHA6B_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8949532.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10513..10644
                     /locus_tag="ACHA6B_001974"
     CDS             10513..10644
                     /locus_tag="ACHA6B_001974"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8949533.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10613..10849
                     /locus_tag="ACHA6B_001975"
     CDS             10613..10849
                     /locus_tag="ACHA6B_001975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8949534.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10861..10947
                     /locus_tag="ACHA6B_001976"
                     /pseudo
     CDS             10861..10947
                     /locus_tag="ACHA6B_001976"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10927..11898)
                     /locus_tag="ACHA6B_001977"
     CDS             complement(10927..11898)
                     /locus_tag="ACHA6B_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8949535.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12550..>12803
                     /locus_tag="ACHA6B_001978"
     CDS             12550..>12803
                     /locus_tag="ACHA6B_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8949536.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT         3854 a         2853 c         2311 g         3785 t
ORIGIN      
        1 aaataatagc agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta
       61 aatctttcgc atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac
      121 catcattaag ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat
      181 taacatttat attatctttg atttgtattt cattcttatt atttttttct tttatgaata
      241 cttttatttc actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt
      301 ttccattcat attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct
      361 ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc
      421 caagaacaga aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct
      481 ctccaattta tatcctacat ttcttatagt agttatacag tctaattgta atttttttct
      541 aagttctttt atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca
      601 tacataatca ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt
      661 taatacttct agttcttttg catttacatc tatttcttca ccatttattt tagctgtata
      721 gctattaaaa ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt
      781 cttaattaaa gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata
      841 tccgtctact aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa
      901 gattagaatt ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat
      961 aaaaggtatc tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc
     1021 ttctcttcca tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat
     1081 tccctctctt atcatattat catcttcaac agttaatatt ttcatatata cctccactta
     1141 agcctataca ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac
     1201 gccttctgtt gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc
     1261 ctttagaaaa aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta
     1321 ctaacacttt ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga
     1381 ccaaaacatc accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg
     1441 gattaacaga taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag
     1501 gcgttcaagc gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact
     1561 gtggatcgat tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag
     1621 tcaaagaaaa gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg
     1681 ggcagacctt ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac
     1741 tgaagcaggc gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa
     1801 gaagtaccgt cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga
     1861 gccgctcaaa cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt
     1921 cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag
     1981 cagtcgccac atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat
     2041 gttcaggatc ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa
     2101 cgtaggcaac cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt
     2161 aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc
     2221 atgaactatc gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca
     2281 tttgttctaa caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt
     2341 tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg
     2401 gtgctgcttg ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc
     2461 gtttcaagca caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg
     2521 tcacaaagga atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct
     2581 cccgcagttc agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa
     2641 tgttcagcat ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct
     2701 gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc
     2761 agtaaatagg cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa
     2821 tcatcgtccc atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa
     2881 aacaagcaaa agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa
     2941 aagtcaaagg tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta
     3001 tcagtccttt tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc
     3061 acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag
     3121 gaatcaaaga tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca
     3181 acagtatcgt tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac
     3241 aggtgaatca acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca
     3301 ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg
     3361 gctgtcacac ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca
     3421 aacaagtaca ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt
     3481 ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca
     3541 tgacaaacgt gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat
     3601 tgaaaaatca agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac
     3661 tggatattct tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac
     3721 gacgtgcacg tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc
     3781 tgataaaaga gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc
     3841 acctcattcg cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg
     3901 atccaataca agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac
     3961 gacaggacac gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt
     4021 taaccgaaag tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt
     4081 atgcttacta tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc
     4141 agcttgtaaa aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt
     4201 ttataaacta tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa
     4261 tagaaaatct gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag
     4321 aaccagaatt ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag
     4381 cagaaaatca attctacctt agatgttctt atatcactct atcagtccta tttgacaaag
     4441 agccagaaat gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt
     4501 tccacaggga ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct
     4561 tcccgatcgt tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac
     4621 cgttgaatgt tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga
     4681 ggaaccggtt caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta
     4741 gcggaaaact cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg
     4801 ataaattccg caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc
     4861 tgagccaccc gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct
     4921 tggtcatgag tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc
     4981 cgctgcagct gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg
     5041 actttcggtt cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa
     5101 agaaactgga catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa
     5161 accttttcct gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt
     5221 tcaaaaacag tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga
     5281 tccttcgtag gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt
     5341 tgttccaatc ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt
     5401 aaagtaacca gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca
     5461 tcaaaaaaca gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag
     5521 ggcttctttt tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt
     5581 cgataagtca gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc
     5641 atcacagcgc tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc
     5701 gtctgaaatc gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa
     5761 aaaatcatca gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata
     5821 ataatcgtag acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta
     5881 attaagcgca tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat
     5941 gcacctacca acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc
     6001 gttaccaaaa tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga
     6061 ttcaaacgag ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc
     6121 gtcaccaaga ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg
     6181 tccgtcagca gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac
     6241 catttagtgg agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca
     6301 aagcagaaca aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt
     6361 gaataatcaa cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta
     6421 aattaggctt ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat
     6481 ttacattact gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata
     6541 agatgacaaa ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag
     6601 catacgctga tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct
     6661 tcatgctggg atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg
     6721 tcaatcccca ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca
     6781 cacctacatt ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag
     6841 ctgccaaatt ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc
     6901 ataaacataa aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta
     6961 cgctgttaac aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca
     7021 atgtccactg gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca
     7081 gcagtaaaaa tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct
     7141 ctttcatccc ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa
     7201 taaccagcaa aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg
     7261 gcgtttattt gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg
     7321 cagtccgtgc gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag
     7381 ccattcagga aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc
     7441 atgagaatcg gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa
     7501 aaagtctggc tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg
     7561 gtaagtgtcc tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat
     7621 gcgatcaacc aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc
     7681 ggcggctcct gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga
     7741 tcgctgagca tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag
     7801 tacttcgtat aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc
     7861 ttgctggtgc tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg
     7921 acagccgtct agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct
     7981 ccgcctgcca aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa
     8041 ctggcaccgc cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca
     8101 gctctacctt gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag
     8161 ataaaatata ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta
     8221 aggttgtata cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg
     8281 agtcaatttg gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt
     8341 cacctttttc aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca
     8401 tttttttgta gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg
     8461 ccgcagccaa tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt
     8521 gatagtcacc ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat
     8581 cggtgataaa ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg
     8641 cccaatccgg aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt
     8701 cagccaacgg accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt
     8761 ctgattcaaa cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc
     8821 cgtattcttt ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc
     8881 ccaagctggc aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt
     8941 ctgccgcagc cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta
     9001 ctgttgctgc acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc
     9061 attcttctcc cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt
     9121 cttgaaaagg tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt
     9181 cccgttaacc ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca
     9241 gtattatgat cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa
     9301 ctatttagcc tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc
     9361 atctaccgga aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt
     9421 aaaagaaacc tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca
     9481 agaagagcgg atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat
     9541 tcatcaaaat ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca
     9601 aactaccaag gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga
     9661 tattttgact cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc
     9721 ggagcatttt gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc
     9781 gatggagtta aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc
     9841 cgttcctttg tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt
     9901 tgccgaagat tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt
     9961 agaaaagttg ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg
    10021 attcctatat aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa
    10081 gaggcaccca tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt
    10141 tgccgatcct tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac
    10201 agcttgcagc gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa
    10261 tatttgcatg cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat
    10321 ttacgaggac agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg
    10381 ggaatggctc gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg
    10441 caggatcaag gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag
    10501 gaagaagttt gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac
    10561 caagctcccc tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac
    10621 acacctttcg gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc
    10681 tggtgatgaa gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact
    10741 ttatcatttt aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca
    10801 gttttttatt gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac
    10861 ttggagcttg gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac
    10921 caagggttag aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg
    10981 attgagccag tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac
    11041 tgacttcagc aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat
    11101 ccataagata aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag
    11161 caaatgagga accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg
    11221 cgctggcgtg tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata
    11281 gcagtggtac ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc
    11341 aatcgtcagc agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg
    11401 cacttcgcag ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt
    11461 atctaagccg gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa
    11521 tgtatccaca tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc
    11581 cagtacggta ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc
    11641 ctgaaccagt gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac
    11701 agcatccttc tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt
    11761 ttcaatctgc ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc
    11821 ctttaaataa acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct
    11881 taatactgtt ccttccatat agatttttga attacacact agtttaaact aaaacacaca
    11941 aggaaaaact taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat
    12001 agactggtca aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat
    12061 tttaatattc taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca
    12121 ctatatcact agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt
    12181 aaatggtcat tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac
    12241 gctgagagta aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt
    12301 tatttcagat taccttaaat gattccattt actttttatc tcattttttc ctactagtta
    12361 gtatcaaata ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca
    12421 gctaaaacct tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt
    12481 ttatggatga tgtctacagt cttttctttt ataatagatt tatgaaaacg aatgcaagga
    12541 gtgatagcta tgatgtctaa aaacaatacg aatggtagaa atcaatttgc gatgcttacg
    12601 attgatgatc tagttcctca agatcatctc gttcgaaaga ttgatgccgc gcttgatttt
    12661 gaatttattt atccaatcgt cgaagccact tactccgatt taggtcgccc aagtatcgat
    12721 ccagtgattc tgatcaaatt ggtgtttatt cagtatttat tcggtatccg ttccatgcga
    12781 caaaccatta aggaagtcga tac
//
LOCUS       DAYYHB010000068        12505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000068 DAYYHB010000000
VERSION     DAYYHB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.069"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..660)
                     /gene="plsY"
                     /locus_tag="ACHA6B_001979"
     CDS             complement(22..660)
                     /gene="plsY"
                     /locus_tag="ACHA6B_001979"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8949537.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            787..1221
                     /locus_tag="ACHA6B_001980"
     CDS             787..1221
                     /locus_tag="ACHA6B_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8949538.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1387..3432
                     /gene="parE"
                     /locus_tag="ACHA6B_001981"
     CDS             1387..3432
                     /gene="parE"
                     /locus_tag="ACHA6B_001981"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8949539.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3444..5894
                     /gene="parC"
                     /locus_tag="ACHA6B_001982"
     CDS             3444..5894
                     /gene="parC"
                     /locus_tag="ACHA6B_001982"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8949540.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6171..8402
                     /gene="pflB"
                     /locus_tag="ACHA6B_001983"
     CDS             6171..8402
                     /gene="pflB"
                     /locus_tag="ACHA6B_001983"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8949541.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8646..9407
                     /gene="pflA"
                     /locus_tag="ACHA6B_001984"
     CDS             8646..9407
                     /gene="pflA"
                     /locus_tag="ACHA6B_001984"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8949542.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9492..10418
                     /locus_tag="ACHA6B_001985"
     CDS             9492..10418
                     /locus_tag="ACHA6B_001985"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8949543.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10600..11493
                     /locus_tag="ACHA6B_001986"
     CDS             10600..11493
                     /locus_tag="ACHA6B_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8949544.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11627..11752
                     /locus_tag="ACHA6B_001987"
     CDS             11627..11752
                     /locus_tag="ACHA6B_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8949545.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11886..>12505)
                     /locus_tag="ACHA6B_001988"
     CDS             complement(11886..>12505)
                     /locus_tag="ACHA6B_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949546.1"
                     /translation="RTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHS
                     DITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILF
                     YSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFV
                     LALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         4494 a         2117 c         2628 g         3266 t
ORIGIN      
        1 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
       61 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
      121 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
      181 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
      241 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
      301 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
      361 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
      421 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
      481 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
      541 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
      601 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
      661 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
      721 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
      781 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
      841 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
      901 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
      961 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     1021 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     1081 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     1141 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     1201 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     1261 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     1321 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     1381 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     1441 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     1501 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     1561 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     1621 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     1681 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     1741 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     1801 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     1861 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     1921 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     1981 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     2041 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     2101 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     2161 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     2221 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     2281 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     2341 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     2401 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     2461 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     2521 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     2581 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     2641 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     2701 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     2761 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     2821 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     2881 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     2941 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     3001 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     3061 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     3121 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     3181 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     3241 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     3301 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     3361 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     3421 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     3481 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     3541 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     3601 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     3661 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     3721 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     3781 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     3841 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     3901 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     3961 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     4021 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     4081 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     4141 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     4201 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     4261 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     4321 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     4381 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     4441 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     4501 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     4561 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     4621 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     4681 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     4741 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     4801 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     4861 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     4921 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     4981 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     5041 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     5101 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     5161 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     5221 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     5281 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     5341 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     5401 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     5461 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     5521 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     5581 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     5641 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     5701 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     5761 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     5821 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     5881 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     5941 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     6001 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     6061 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     6121 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
     6181 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
     6241 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
     6301 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
     6361 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
     6421 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
     6481 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
     6541 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
     6601 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
     6661 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
     6721 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
     6781 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
     6841 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
     6901 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
     6961 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
     7021 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
     7081 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
     7141 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
     7201 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
     7261 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
     7321 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
     7381 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
     7441 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
     7501 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
     7561 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
     7621 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
     7681 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
     7741 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
     7801 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
     7861 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
     7921 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
     7981 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
     8041 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
     8101 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
     8161 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
     8221 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
     8281 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
     8341 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
     8401 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
     8461 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
     8521 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
     8581 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
     8641 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
     8701 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
     8761 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
     8821 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
     8881 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
     8941 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
     9001 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
     9061 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
     9121 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
     9181 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
     9241 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
     9301 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
     9361 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
     9421 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
     9481 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
     9541 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
     9601 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
     9661 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
     9721 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
     9781 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
     9841 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
     9901 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
     9961 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    10021 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    10081 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    10141 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    10201 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    10261 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    10321 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    10381 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    10441 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    10501 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    10561 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    10621 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    10681 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    10741 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    10801 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    10861 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    10921 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    10981 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    11041 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    11101 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    11161 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    11221 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    11281 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    11341 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    11401 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    11461 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    11521 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    11581 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    11641 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    11701 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    11761 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    11821 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    11881 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    11941 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    12001 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    12061 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    12121 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    12181 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    12241 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    12301 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    12361 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    12421 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    12481 aaatgtaaaa aattagtggt tctac
//
LOCUS       DAYYHB010000069        12288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000069 DAYYHB010000000
VERSION     DAYYHB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.070"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..458
                     /locus_tag="ACHA6B_001989"
     CDS             138..458
                     /locus_tag="ACHA6B_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949547.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            910..1110
                     /locus_tag="ACHA6B_001990"
     CDS             910..1110
                     /locus_tag="ACHA6B_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8949548.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1257..4046
                     /locus_tag="ACHA6B_001991"
     CDS             1257..4046
                     /locus_tag="ACHA6B_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8949549.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4093..4620
                     /locus_tag="ACHA6B_001992"
     CDS             4093..4620
                     /locus_tag="ACHA6B_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8949550.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4641..5831
                     /locus_tag="ACHA6B_001993"
     CDS             4641..5831
                     /locus_tag="ACHA6B_001993"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8949551.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5871..7169
                     /gene="asnS"
                     /locus_tag="ACHA6B_001994"
     CDS             5871..7169
                     /gene="asnS"
                     /locus_tag="ACHA6B_001994"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8949552.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8222..8896
                     /locus_tag="ACHA6B_001995"
     CDS             8222..8896
                     /locus_tag="ACHA6B_001995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8949553.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8893..9234
                     /locus_tag="ACHA6B_001996"
     CDS             8893..9234
                     /locus_tag="ACHA6B_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949554.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9264..9623
                     /locus_tag="ACHA6B_001997"
     CDS             9264..9623
                     /locus_tag="ACHA6B_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8949555.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9883..10005
                     /locus_tag="ACHA6B_001998"
     CDS             9883..10005
                     /locus_tag="ACHA6B_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949556.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            10147..12228
                     /locus_tag="ACHA6B_001999"
     CDS             10147..12228
                     /locus_tag="ACHA6B_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8949557.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4081 a         2147 c         2662 g         3398 t
ORIGIN      
        1 aaagaggttg tgacgtggtc acgacctctt tttgacgatt atgatacaaa aaacatcatt
       61 tatgaacaaa atgtgaataa cgggcgttct tgagatatga ttttaattgg gaaaaactta
      121 tcgataagga ggtctttatg aaaaaaagac tgttatggtg tttgctgttt ctatccctaa
      181 gtttaacaag tcagccagtt tttgctgaag aaacacagcg gtctcctatt acaggcacac
      241 gtctgtacaa aaatgtttat gtacagacaa cagatcttga ttctgcgatt cctgaaacag
      301 aagaacggtc tttgcagcca agatttggtt acttttggta ctgtccgacc tgtgattatg
      361 aaagttgctg gcatttctta cattctacag ctgcagcttt tgcttttact catactcaga
      421 aatatgatgg acatcatcct gttatttatg aggtatagat atgagcaaaa aagaaaaact
      481 aatctcgttg atcgtattgg tttgccttgt tcttctggta actgtcatag gctatcagaa
      541 ataccatgag aacatcagac ctactaaaga atcttcccca gcctcgaccg ttgaaacaga
      601 accgataagt agtcagaaag aataattttt ttagtttaaa gaagaacatc agagcaaaaa
      661 aacaccaact gaacaatatg tcgtttattt taaaaaaagt tcggtttttc actttatgta
      721 atcgctgaga aaactttttt taataaaaaa agtcgaaaaa ctctagcttt tttgtaaacg
      781 gtatgatata ctataggagt agtttttgtt cggtaatctg tggtagcttg tttcaaataa
      841 acatcttcca gatatacacc attcaagtaa ttgcaaaata tgtgttgata cacaaggagg
      901 atttttatta tggaaacagg tacagtaaaa tggtttaact cagaaaaagg atttggattc
      961 atcactgcag aaaacggaaa cgatgtattc gttcatttct cagctatcca aggcgacgga
     1021 ttcaaaactt tagaagaagg ccaagcagta actttcgatg ttgaagaagg ccaacgcgga
     1081 cctcaagcaa caaacgttac taaagcttaa ttaacctata ctaaaaaata aacgcccaga
     1141 catttgcgtg tctgggcgtt tttgtctatt ttctttaaaa agcgatgaga gtatttttct
     1201 gcactttgta gtataatttc aaaagaacat tagaggcagg agaagaggaa gatattttga
     1261 aaccaagaac ttatgcagtc gttgatatcg aaacgactgg gacgaatcca aaagaagatc
     1321 ggatcatcca atttggctgc gtcatgattg aatcaggtcg gatcacttca cgtttttcga
     1381 tcgatattca tccagggcgg aagatttcaa aacaaatcca gcatttaaca gggatcacga
     1441 atcaacgcgt ccaaaaagcg ccttatttcg aagatgtcgc ccaaacgatt tacaatcttt
     1501 tggcagacac catatttgtc gcgcataata tttattttga ttataatttt ttgaaccatg
     1561 aactcatgcg atgcggactt cctagtttga agataccagg catcgataca gtagaacttg
     1621 cacaaatttt cttgccgacg gaaccaagct ttcgcttagc tgatttgtca gaaagccttg
     1681 gatttattca tgagaatcct catcaggcag atagtgatgc cgaagtgacg ggtcaacttc
     1741 ttttgctgat cgaagaacgg atgagaaaat tgccaatcat cacgttagag cagattgcgc
     1801 gattaagtag acacacaggt atggatacga gtcgtttcat ttatcatgtg attgaagaaa
     1861 tgaaagaaaa accagagcca cttgatccct cattagaaat cgtcgatggg ctagctttgc
     1921 agaaaaaaga agtggaacta ttcacatccg tccattatgg cgagcggaca tatccaagaa
     1981 aaaaacaagc aaaagaaaaa atgtttggta aaacactgat gtatcgaaaa gaacagaaca
     2041 gactgatgaa tgcggtctac gaccatttca caaaagatga atcaaaagat ttgatgatcg
     2101 aagcagctac aggaatgggt aaaacaatcg gttatctgct gccattgagt tatttggcta
     2161 cacctgaaaa acccgctgtc atcagtaccg tttcgctagt tttgcagcag cagatcatag
     2221 aaaaagacat tccattgctg aatcaattgc tagatcagcc catacaacct gtaattatca
     2281 aaagctatcg ccactatatt gatttacagc gttttaaagg cacacttgtg gaaccgccag
     2341 aacaaaaaca atatgctttg tatcaaatgg cggtcttagt ttggctgacg caaacgaaga
     2401 ccggtgattt ggatgaatta catttaacaa atttgaacca ttcattcttt gcagatatcg
     2461 cgcacagagg tattggattt ttagcaagaa atcagtcatt ttacgaacaa gactttgttc
     2521 gtttcttgca aaagaaaatc aggcaaagta attttttgat tgtcaatcat gcgtttttaa
     2581 tacaagaaac acagcgaaaa gaaccattgc ttccagctag tccatttttg ttgatcgatg
     2641 aagtacatca tttgccggaa atcgctgaaa aagtaaatga caaacgatta agtattaact
     2701 atttccataa acaaatccag caattagaag aaccaaatca gctgtttgac cgaatacaaa
     2761 gctatttacc gaaagaacat gaagccttac gctggttatc gatttatcga gaagaacttt
     2821 ctgcgctatg cgaagcacag atttgtatgg cagaaggatt aatgagttta gtgagcggca
     2881 agcaacctaa cggacagtat ccagaagaag tcatgatgac aaaagagatg ttggatcagc
     2941 tgccactgga atctgggaca gccattgatc acctgcagct tttttatcag gagatccagc
     3001 agctacagtc acaaatgcgt ctgacttttg agattatcca agaaagctgg acaaaccaga
     3061 tgcggatcga tttagcaagt ttatatagct tatttgatca attagacaag cagtacttgc
     3121 tgatggatca atggctggaa gactggcgag atcatttgat ccattggttc gttcctggga
     3181 acacagcgca gcagatgatt tttcatctcc atgattttca agcggctatg ttgccgtcga
     3241 ctgtttggta tcctcgatat gatcgtattc tttatattgg aggaacgctg aagatcggcc
     3301 ctaatcgaca ctatctctct aagcggcttg gcattgaagc agcacctctt aaagtgattg
     3361 cctctcctta tgattatgag aaacaggcaa ggatctatgt gccaaaagag gcacctgccg
     3421 tccatcaagt cagcaatcaa gcatatatcg attatttaga gacagcactt acaggtctga
     3481 tcgaacaaga agatcggcca atacttgtgt tattcacttc tcatgatgtt ttgcagaagg
     3541 tctatcagaa aatgcatctg tctatgctag aaaaaggacg agaaatacta gctcaagggt
     3601 ttggcggaag ccgggagaag cttttgaaac gcttcatttt gtcagaaaaa agcgtattgt
     3661 ttggcgctga cagcttttgg gaaggtgtag atttgcctgg ggatgctttg cagatcgttg
     3721 tagtcacacg tttacccttc gataatccgc ttcgcccgat ggtcaaagct aggaacgctt
     3781 atttagaatc agaggggatc aatccttttt accaggaatc cgtacctaaa gcagctttga
     3841 agttacgcca agcattaggt cgcttgataa ggacggagac agatcgtggc gtattgctta
     3901 ttttggatcg aagactagtc actgcaaaat atggtgcacg gatcatccat gcattaccga
     3961 aaaaagtacc gatcaaagag ctagctatgg aggaaatcta tcaggacatc catgaattct
     4021 taaagaaaaa tgaagaagga agttagagga gctgtctttt ttcataatta aaatctgcta
     4081 taatgaaaag gaatgaagat aaaggagcga cgacccttga agaaaaaaca gacaaaggaa
     4141 aaaacgatta atcgcgtgtt acttggcttg atcgtatttt tattagcggt catcgtgatc
     4201 agctcgattt tttacttgcg ttccagtcgt ccggcggtgc aggcgaagaa agaagcagta
     4261 gaaatcgcaa agaaatatgc aaatcttgag gaagccgatc atttttattg gtttactcga
     4321 aagaagacat acttttcact gacaggtaca aatgataaag gaaaagaaat cgccgttatc
     4381 attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa caaagggact gactgaaaac
     4441 gaagcaaaac agacaattgc tgcggcccat cctgaaatcc aaatcgaaaa agctgcttta
     4501 gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa aagaaacaaa tgggaaactg
     4561 aattattacc tgattggctt tgaatctgga gatgaagtca agacaatcaa gggaatttaa
     4621 aaggtaaagg aatgaatggg atggagttat caaaaagaac aaaacgatta gaaccgtctg
     4681 tcactttggc agctgcggca aaggccaagg cattaaaagc aaaaggcaga gatgtgctga
     4741 gtttgactgt gggagaaccg gattttgcga caccagaaaa catccaagaa gcagcaatcg
     4801 aagcaatccg caacgggaaa gctagctatt atacaccaac agcaggaatc ccagagcttc
     4861 gacaggcaat cgttgattat ctgaagaaaa actatggact tgtctatgaa ccttcacaaa
     4921 cagtcgtaac agacggagcg aaatttgctt tgtataccct tttccaaacg atccttgacc
     4981 cgcaagatga agtcatcatt cctgtgcctt actgggtcag ttacggtgag caagtaaaac
     5041 tggcagaagg acttcctgtc tttgtgaaag gggaagaatc caatagctgg aaagttactg
     5101 ttgaacagct agaacaagca cgttcagaaa aaacaaaagc cgtaatcatc aattctccat
     5161 cgaatccgac tggaatgatt tatagcaaga atgaactgca ggcaatcggt gaatgggcag
     5221 taaaaaatga tattttgata gttgctgatg atatttatgg tcgtttagtt tataatggca
     5281 atgagtttac tccaattgca acgatttcag aagcaatcaa aaatcagact atcatcatca
     5341 acggcgtgtc taaaacatat gcgatgacag gctggcgcat tggttatgcg gtgggaaatc
     5401 ctgtgatcat caatggaatg atcgctgttg cttcccagtc aaccagtaat ccgacagcag
     5461 tcagtcaata tgcagctgtt gaagcattga aaggcgagca agatacagtg gaagaaatgc
     5521 ggaacgcctt tgaagaacgc ctgaatacgt tatattcgct tgtggcagaa ctgccgggtg
     5581 tttctcttga aaagccacaa ggagcttttt atctttttcc taatgtaaaa gaaacgctac
     5641 gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga tcttttagaa gaaacagggg
     5701 tagcgcttgt gactggcgaa ggctttgggg caccagaaaa tgtccgcatg agttatgcta
     5761 ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa gcaatttatt gagagtaaaa
     5821 gtcaaaatta atcctaaaga aattcaagga atataaaagg agagtcatac gtggaacaaa
     5881 ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt aacaatcggc gcttgggtcg
     5941 caaataagcg atcaagcgga aaaatcgcat ttttacaatt aagagacgga acagcctttt
     6001 tccaaggtgt agttgtaaaa agcgaagtgt cggaagaagt tttccaaaca gctaaaaatc
     6061 tacaccaaga aacatccgtg ttagtaactg gagaaatccg tgaagacagt cgttcaaaat
     6121 ttggttatga aatcggaatt acaaatgtgg aagtggtaag cgagagccac gattatccga
     6181 tcacaccaaa agaacacgga acagacttct tgatggatca tcgtcattta tggctgcgtt
     6241 cttcaagaca acatgcgatt atgcagatca gaaatgaaat cattcgtgca acgtatgaat
     6301 tctttaatca acatcatttt gtcaaaattg atccgccgat cttgactggt tctgcaccag
     6361 aaggcactac ggatctattc gaaacaaatt actttgatca aaaagcttat ttatctcaaa
     6421 gtggccaatt atacatggaa gctgctgcta tggctttcgg aaaagtcttt tcattcggtc
     6481 cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt gatcgaattc tggatgatcg
     6541 aaccagaaat ggctttcact catcaagaag aaagtttaga agtacaggaa caatatgtag
     6601 cattcttagt acaaaatgtt ttagatcatt gcgaatatgc attagatgta ctaggacgag
     6661 acaaagaagt cttaaaaact tatacaaaat tgccattccc tcgtattact tatgatgatg
     6721 cgatcgaatt actgaagaaa aatggatttg atgacatcga atggggcgaa gactttggtt
     6781 cacctcatga aacgttcatt gcgaattcat tcgaacagcc tgtctttatc ctaaactatc
     6841 caaaagccat caagccattc tacatgaaac cacatccaac aagagaagac gtggtgatct
     6901 gtgcagatat gatcgcacca gaaggttacg gtgaaattat tggcggaagc gaacgggcaa
     6961 cagactatga tttcttgcta gaacaaatcc gtaatcacgg tttggatgaa aaagaatatt
     7021 catggtatct tgatctacgt aaatacggtt ctgttccgca ctctggtttt ggtctaggtc
     7081 ttgaacgaac agtcacttgg attgctggta tcgaccatgt acgtgaagct attccattcc
     7141 cacgtttgtt gaaccgaatt tatccataag aggtgatgaa aaagctgttc tgcatcaagt
     7201 aattagaaca gccaagcaaa aacagctgtt catgtttttg aattggcggg acttattacc
     7261 gcagatgtaa gcttttgaat accgtttagt ggagaggttg tgaaaaagct gtttagtttc
     7321 gagtattaag aaaaattttg tggaaatggc tttccacatt tttgcataaa tttggcttaa
     7381 taccgagaaa ctggcttttg aacaccgttt aaggaagagg ttgtgcgcct gccgttcagc
     7441 tccgagtcac aaggagtaat ttcaaaaaac agtttttcat gtttgatgac tattgtgact
     7501 tgtgaccgag gagttggccg gagagcaccg tttattctaa aaaagaactg cgaactctcg
     7561 cagttctttt tttaaaatac gggataaagg aagtttccag cagacaagag aagaaagcaa
     7621 aagaaattgc tatttgattc acaaggttct ataacgaaat cgcaatcaat caaacattta
     7681 caaagaaggt aattcaaatg gaaatctgat ttgatgatgc ttctgctgtt gaaataaagc
     7741 agcatctagc acctgaaaac aaactaatag taacattcga agatggagta ggtccgtatt
     7801 ttcagcatgc aataatccat atgtaagttc aattcaccct tgaaatcatc ggaccagata
     7861 aagttatgac caatctatcc caagcaatct agaagagata ttagtaaagg gacatttact
     7921 agaagtctta gatgagccta taaatatatg ttctgatggc gtcatcagtg gtctgattga
     7981 cggaagctta ggtgtgatcg atctctctgg accaaacaac gaaggaatca aggggaatcc
     8041 ggcacgttaa gaaaaaaata gcataaaaaa ctagacactt gatgagcgtt cattcaattg
     8101 tttgaaggga ctaggctggt gtcatttttt atacatatat tttctctttc gtcttgatag
     8161 ggcttatggg ataatataag gaagaaaatc atcgttttat tcaaaaagga gtttttgaca
     8221 aatgaaaaaa cgtatattat ttatcggtga tagcattaca gacgcaggaa gagaccgagc
     8281 agaagcaact gatctaggta aaggttatcc acttttgatt gccggaaaac taatctccca
     8341 atatccaacg atggaatttg aatgcatgaa ccgtgggata ggaggagaca aaattgcaga
     8401 tctgcaagta cgctggcaag aagattgttt agctttacaa cctgactatg tatcgatttt
     8461 gatcggaatc aatgatacct ggcataatac tagcgatcag gctatattcg gaactgagaa
     8521 atctgctcag caatttgaat ccgtttatcg aaaattacta aatgaacttc aaacagtggg
     8581 aatctgcaac atcatcttga tggagccgtt tgtacttcct ataaccgacg accgtcatgg
     8641 ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt aaacttgcta aggaatatgg
     8701 cgcattgctg gttcctttag atggtatatt caatgcttta gggattgttt atggtttttc
     8761 gaaatataca agagaagatg gggtccaccc aacgattggc ggacatgaga tcattgcaca
     8821 aagctggata gatgcaattg ccgcagtcga tttttttagt ggagataaaa atgagcgaaa
     8881 gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag aagatctacc tcaaatcaat
     8941 cagctgatta ctgatcatcc agaatcattc gtaaaagcaa aaacggcagc tgacctaaat
     9001 gataagaata aactattgct cgttgctgta acaaaagaaa aacggattat cggatttgcg
     9061 caagcggacg tgcaacagtc agaagaacga ttggatcagc gaccatcagg aaaactaatc
     9121 caacacacat tagtaacaaa agaaaatacg gaagaaatcc aaaaaaaatt tgcagaatat
     9181 ttgatactgg aagctaaaaa aagaagaata aaatcatttt ttgcccagtt ttaatcaaaa
     9241 acagtaatag aaggagaaat acaatgagtt gtttaggaaa tgtcatctgg tttatattcg
     9301 gcggactaat cggtggtctt agctggttag cagcggggat actttggtgt atcacgatca
     9361 taggtatccc aatcggtatg caatgtttta agatcgcctc cctttctttt gcgccttttg
     9421 gcaaagaagt aatttatcac cagtctggca tgtcattgat tgttaatatc atctggctga
     9481 tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag cggcatcctt ctatgtatca
     9541 cgatcatagg gatacctttt gccaagcaat ctttcaaact tgctcgattg gcgttgatgc
     9601 cattcggtgc agaaattgta taaaagagaa taataaaggt tagataaagc atagagtcag
     9661 gtgggataca tgtatttcac ctgactctat gcgttctcct gtataagttc ttccatgtga
     9721 cttttacgtc ttttatttat atggggttac tgtttatgga aagttgaaat aattttcatt
     9781 aaagaactct ttctatattt ttgaaagcgt agtggagctt cgaatatctg ggaaggacat
     9841 tttgcaggga ttgaaaaaag atattgtcct atcgaaaaat taatgaattt agacaaattg
     9901 ccagcaacag gcttcaaact tagttgctat cctgtcaaga tcaagaaggc aagtgcgggt
     9961 tggataagag cagtggcaat gatcgaaaag aaaaagaaag aataagccaa aacatctgta
    10021 tggaagaaat tttccgatgc agatgttttt cattttttct ttcggatacc ttgacatatg
    10081 aataaccgtt catttataat tagttatgaa gagatattca tgtgttgagg cgaggaggtc
    10141 attaaaatga aaagttacag attagaaggt ttagattgtg cgaactgtgc aatgaaaatc
    10201 gaaaaaggag ttcagaagat caatggagtc aaggaagcaa cggtaaactt cacttctgga
    10261 aaactaacga ttgacgcaga ggaagatcat ttggctacga ttgaacaaga aataaaaaaa
    10321 gtagtgaagg aattagaacc agatgtcaaa gtaacagaaa tagataagga aaaagtatct
    10381 gaacatggaa atgaaaaaga gagaaatacg ctgttccgga ttttattttc ccttgcaggt
    10441 atcgctttat tgctattatt tgattttaat gaaccaattc gcttgatagg gtatttattg
    10501 atttatctgc tgataggata tgatgtagtg aagaaggcag tgatgaatat cgtcaaagga
    10561 aaaatctttg atgagaattt tcttatgtct gttgctacga ttggcgctat gttgatcggt
    10621 gaatatccag aagccgtagc agtgatgcta ttttatcaaa tcggcgagta tttccaagga
    10681 ttggcagtca gtcattcaag aaaatcgatt cgcgaattga tggcgatccg tccagaaaca
    10741 gctcacgtgc agacgacgga aggattgatg accgtgaacc ctgaagacgt gcagatcggt
    10801 cagtttgtac ttgtgaagcc tggtgaacgt gtaccgttag atggaacgat catcgaagga
    10861 gaatcattag tcgatacgtc agcacttaca ggagaatcag tccctaaatc tgtatatgtt
    10921 ggtgaaacag tattaagtgg atttatcaat aaaaataaac catttttagt tcaagtagaa
    10981 aaatcttatg aaaattctac tatcagcaaa ttattagaat tagtagaaaa tgccagcagt
    11041 aaaaaagcac cagctgaaaa tttcattact aaattcgcgc gctattacac accagtcgtt
    11101 gtaggattgg ccgttttatt agctgtttta cctccacttg ttgtttctgg agcggcgttt
    11161 tctgaatgga tctatcgagc attgacattt ttagtgatct catgtccatg tgcattagtg
    11221 atctccgtac cccttagttt ctttggcggt atcggaggag caagtaaaat cggtgtatta
    11281 gtaaaaggtt caaattactt ggagttgctt gcacaaactg agacagtggt attcgataaa
    11341 accggcacat taacgaaagg tgacttttcg atccagacga tcgatacaaa aatcgatcca
    11401 aagatattca tgcaatatgt tgcgagtgca gaacagtttt ctacacatcc gattgctcaa
    11461 tctgttttag aaggctacgc aggtccgctt ttaccaacag ctaatatcca agaatttgca
    11521 ggtgaaggga tcttagctga agttgatggc aaacaagtat tagcaggtaa tcacaaattg
    11581 atggaaagat ttgaaatctc ttttccttca agtcaagaaa tcggtacatt gctttatcta
    11641 gcgatcgatc aaagttacag tggttatctt gttattgcag ataccttgaa agaagatgcc
    11701 gtagacgcat tggttcaatt aaaaaaggct ggtgtgaaga acacagtcat gttgacgggc
    11761 gattcgaaaa aaattgctga tcatatcggg aagcaagtag gcgtggacaa gatatatagc
    11821 gaattgcttc cagaagataa agtgcagcgg ttagaggaaa tcttacaaag aaacaataaa
    11881 aagaccgcat ttgtcggaga tggaatcaac gatgcaccgg tgctagcaag agcagatgta
    11941 ggaatcgcaa tgggtggatt gggaagcgat gctgcaattg aagcagcaga tgtagtcatc
    12001 atgaatgatc aaccatcgaa gattgcagaa gcaatccatt tagcaaagaa aacattgaaa
    12061 atcgtcaaac agaacattgt ctttgctatt gggataaaaa ttttggtact tgccttaggt
    12121 gcttttggtt ttgcatcaat gggagatgca gtatttgcag atgttggggt gacagtatta
    12181 gctgttttga atgccatgcg aagcttgcat gtaaaaaata aacgttgatc aatgaagagg
    12241 acgaatgaac gtcctctttt ttttatatcc ttttcttaat gaaaaatg
//
LOCUS       DAYYHB010000070        12199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000070 DAYYHB010000000
VERSION     DAYYHB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.071"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="ACHA6B_002000"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="ACHA6B_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8949558.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACHA6B_002001"
     CDS             complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACHA6B_002001"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8949559.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACHA6B_002002"
     CDS             complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACHA6B_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8949560.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACHA6B_002003"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACHA6B_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8949561.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="ACHA6B_002004"
     CDS             3621..4100
                     /locus_tag="ACHA6B_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8949562.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="ACHA6B_002005"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="ACHA6B_002005"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8949563.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="ACHA6B_002006"
     CDS             5401..5529
                     /locus_tag="ACHA6B_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8949564.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="ACHA6B_002007"
     CDS             5533..6078
                     /locus_tag="ACHA6B_002007"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8949565.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACHA6B_002008"
     CDS             complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACHA6B_002008"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8949566.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACHA6B_002009"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACHA6B_002009"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8949567.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACHA6B_002010"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACHA6B_002010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8949568.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACHA6B_002011"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACHA6B_002011"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8949569.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3342 a         2527 c         2204 g         4126 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actacggttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttatt
//
LOCUS       DAYYHB010000071        12132 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000071 DAYYHB010000000
VERSION     DAYYHB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.072"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHA6B_002012"
     CDS             <1..610
                     /locus_tag="ACHA6B_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949570.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHA6B_002013"
     CDS             723..1001
                     /locus_tag="ACHA6B_002013"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949571.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHA6B_002014"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHA6B_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8949572.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHA6B_002015"
     CDS             1458..2213
                     /locus_tag="ACHA6B_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8949573.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA6B_002016"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA6B_002016"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8949574.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA6B_002017"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA6B_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8949575.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA6B_002018"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA6B_002018"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8949576.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA6B_002019"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA6B_002019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8949577.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA6B_002020"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA6B_002020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8949578.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHA6B_002021"
     CDS             6950..7492
                     /locus_tag="ACHA6B_002021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8949579.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA6B_002022"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA6B_002022"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8949580.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHA6B_002023"
     CDS             9085..9987
                     /locus_tag="ACHA6B_002023"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8949581.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA6B_002024"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA6B_002024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8949582.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHA6B_002025"
     CDS             11430..11852
                     /locus_tag="ACHA6B_002025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8949583.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4034 a         2155 c         2677 g         3266 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt tt
//
LOCUS       DAYYHB010000072        11950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000072 DAYYHB010000000
VERSION     DAYYHB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.073"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(369..1487)
                     /locus_tag="ACHA6B_002026"
     CDS             complement(369..1487)
                     /locus_tag="ACHA6B_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949584.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1720..1842
                     /locus_tag="ACHA6B_002027"
     CDS             1720..1842
                     /locus_tag="ACHA6B_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949585.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1958..3265
                     /locus_tag="ACHA6B_002028"
     CDS             1958..3265
                     /locus_tag="ACHA6B_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8949586.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="ACHA6B_002029"
     CDS             3384..4583
                     /locus_tag="ACHA6B_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8949587.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="ACHA6B_002030"
     CDS             complement(4610..5878)
                     /locus_tag="ACHA6B_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8949588.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5933..5999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6041..6688)
                     /locus_tag="ACHA6B_002031"
     CDS             complement(6041..6688)
                     /locus_tag="ACHA6B_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8949589.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(7096..7599)
                     /locus_tag="ACHA6B_002032"
     CDS             complement(7096..7599)
                     /locus_tag="ACHA6B_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8949590.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7722..8486)
                     /locus_tag="ACHA6B_002033"
     CDS             complement(7722..8486)
                     /locus_tag="ACHA6B_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8949591.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8594..8869
                     /locus_tag="ACHA6B_002034"
     CDS             8594..8869
                     /locus_tag="ACHA6B_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8949592.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(9070..9417)
                     /locus_tag="ACHA6B_002035"
     CDS             complement(9070..9417)
                     /locus_tag="ACHA6B_002035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949593.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            9585..10514
                     /gene="yidC"
                     /locus_tag="ACHA6B_002036"
     CDS             9585..10514
                     /gene="yidC"
                     /locus_tag="ACHA6B_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8949594.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            10635..11950
                     /locus_tag="ACHA6B_002037"
                     /pseudo
     CDS             10635..11950
                     /locus_tag="ACHA6B_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         3689 a         2407 c         2168 g         3686 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct ggcagttcgt
     6541 gatctaatat gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt accgtttttg
     6601 gatcattgac agatgagtac gcatttaata actgttctac ttctcttatt ataaaaaaat
     6661 attggtatgg ttttattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc
     6721 atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcctggactc
     6781 ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgctta ttattctatg
     6841 tacatgattc attcgataaa taaaagtaaa ccgtatcttg aacagatagt caactgcaaa
     6901 cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat gaatggtgtt
     6961 caaaagcttg catctgcagt aataagccga aacttcacaa aaatttgaga agtaattttc
     7021 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcaa tatggtctat
     7081 tattggtttt acctgttaaa tcgaggattc tttcccaaag taacgttgcc atctcttacg
     7141 catacttgca gacatgggct gagccgcttc tttgactgcg cagtatttgt ccacgaatgt
     7201 cacaatatat ccttctttat acttaggcgg tgcgatcgtt gcaccccaca tgtgcttcaa
     7261 aatgatatct cgctcaagat ccgacaaatc ggtgattttt tctgcattct ttacagcaat
     7321 tcttggatgg atatacgcat gtgtcccctc gtcaaatttc gtcgtacgcc aatcatagta
     7381 gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta gcattgccac cccatttttt
     7441 tgcgagcaga taactatagt aagaaacact gatggaatgc tccaaacgag tggaatgaac
     7501 atgctgtgtg taattcgcta attttttcac tgcttctgtt tctaaaagat cttgtacata
     7561 agacatatat tcttcgtctt cacgccattt ttctgtcata attatcgatc tgccaccttt
     7621 ttgaatttca ttttcttctg aaattgatct aagtatacca ccagtgttcg gattattcta
     7681 gtcgctctag ataaaatagc taaagcttaa tagttcctta atcataaaga aaaatgataa
     7741 agaacgatcc ctgctgcgac tccgacattc aatgattccg catttccttt cattgggata
     7801 tatacgttct gatcagtcaa agacaagatt tcctgactga ctccctgacc ttcgtttccc
     7861 atgattatag cgaaatcctc tgttttttct actttgtcta atgcatgtgc ttcttcattt
     7921 aattctgtac cgtacaccgt aaaccctttg cctttcatta cagggattac atctgccaaa
     7981 tctttttgta acacaggcaa atgataattg cttccctgca tgcttcgcag cacctttgta
     8041 ctataaatat ctgccgttcc tttacctaat aaaacaccag ccataccaaa cgcatctgcc
     8101 gtacggatca tcgttcccac atttccagga tcctgtacat tatctagtaa aagccagcct
     8161 cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca ctgctaagat cccttgcggt
     8221 gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa ctagaaaagt agaaatctct
     8281 ttgtttacag ccagccagtc tccccattct ttcatacctc tctgtgtcac aaaaagttct
     8341 tttatttctg catcagacga cgcagcttct tcgatcagat ggaacccttc caaaagatat
     8401 tctgcctttt cttctctgta tttcttttta tgtagttttt ttaattcttt gattcttgga
     8461 ttttttgctg attggatctc tttcacattc cttcacctca cctgattata tcatggaata
     8521 ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa aacaggaaag
     8581 aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc agggcgtggg
     8641 atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa cggtaaaaaa
     8701 cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca tccaaaaatt
     8761 tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg tggatgtcca
     8821 agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag gttgtgaaaa
     8881 ggtcgcttag ttttgagtat taaggaaaat tttgtgaaaa tggctttcca catttttgca
     8941 taaatttgac ttaataccga gaaactgact tttgaacacc gtcgataagg ttgtttttga
     9001 agcgctttgc ctcgagtatt aaggagaaac attttcaaaa tagcttctca tattttgaaa
     9061 tgtaatgact taataccgag aggcagctga taaaacaccg tcaataaggt tgtgaaaaag
     9121 ctggtctgca tcaagcatta aggactggtt gtgaaaaagt cgcttagttt tgagtattaa
     9181 ggaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgtctta ataccaagaa
     9241 actgactttt gaacaccgtc gataaggttg tttttgaggc gctttgcccc aagtattaaa
     9301 gacaggtagt aaaaaagctg atctatcact tgcagtgagt tcatttgact cctctatttc
     9361 gggcagaata aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct
     9421 tttctttcca agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa
     9481 aaagaaagaa tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta
     9541 gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa
     9601 actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga
     9661 tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta
     9721 tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta
     9781 ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact aggtatcagt caatcaaaaa
     9841 aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacaac
     9901 aaaaactaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcaac
     9961 aagtatatcg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga
    10021 tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg
    10081 aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtt gccattgcag
    10141 gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa
    10201 agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgttttcatg tcgatcagtg
    10261 cgccagccgg tgttacactt tactgggtag tgggtggtgt gttcagttgt ctgcaaacat
    10321 tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac
    10381 aaaatccgcc aaaacaagtc gtcactccaa gaaaagacgt gacagaatcg gcaaagccag
    10441 ctgacaataa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
    10501 aaaagcggaa ataaaaatat atgacaaaaa actgtagaca aataaaaaaa tttctttgtt
    10561 ctacagtttt tttgttacaa tcaaagaaaa cgtcgctttt cgaggaggaa tgtataatga
    10621 tgtccaaata atcaatggat agtcgaatgg aagaagccaa aatttcgcta gatgcacttg
    10681 ttcctaaaaa tcatttggtc cataaaattg acaaaatatt gttatttgat tttatttatc
    10741 caattgttga atcaacttac tcgaccattg gtcgtcctag cgttgatcct gtcatcttag
    10801 taaaattagt attcattcaa tatttattta ggattcgttc gatgcgtcag tccattaaag
    10861 aagctgaaac aaacctagct tatcgttggt ttcttgggct tagtttaatc gataaaattc
    10921 ctcatttttc aacgtttggt aaaaactatg tacggcattt cagagaaacc gattgtttga
    10981 acagatattt atcattatcc ttgaacaagc agttgaagct agatttattc atgaagaaat
    11041 tttgtatatg gattcaacct atattaaagc gaatgcgaaa aaacatattt ttacaagaga
    11101 aatggtacat aaataagcca ttttatacca aaaacaattg aaagaaaaaa ttaatgaagc
    11161 aagaattgca gaaggcaaac gtccttttgg ttcaatcatt catcaggagg tggtagaagg
    11221 gaaaattagt gtcgcagatc cagaaagtgg ttattatgta aaaacagaac aagaaaaaca
    11281 gtttgcttat tctgctcata ccgtttgtga tgaaaatggt tttgtattag atgtcatgat
    11341 tacgcctgga aatctacatg acagccgaat gctcgtgcca caaatcgaac gtgttaaatc
    11401 atgttgtggt gtagctgctg atgctgcgta caaaacacct tggaacgcca agtatttaat
    11461 cgatcgaaaa ctacgaccca tttttcctta tactcgaccc aaaagaagca aagaacgttt
    11521 taaaaagaaa gatttttatt atgatcccta ttataactgg tacctatgcc caaatgatca
    11581 aacacttcag tttcgcacaa ctactcgaga tggttataaa aaatatgtat ctaaatcttt
    11641 tatttgtgaa agttgtctat tattgaaaaa ttatacagaa agccagaaac accagaaaga
    11701 aatacatcga catatttagg aagactactt agatgaagca gaacatttac gccatacggt
    11761 gtacaatcgc gaaaaatata aaaaaaagaa aagaaacgat tgaacgcgtt tttacagacg
    11821 caaaggaaaa gcatggaatg cgctacacca aatatcgagg cttggaaaaa accaggtgca
    11881 tacgatgctt gtttttgccg caatgaatct gcaaaaatta gccatttggc agtgggggca
    11941 tatgtcttta
//
LOCUS       DAYYHB010000073        11650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000073 DAYYHB010000000
VERSION     DAYYHB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.074"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHA6B_002038"
     CDS             complement(105..629)
                     /locus_tag="ACHA6B_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8949595.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA6B_002039"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA6B_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8949596.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA6B_002040"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA6B_002040"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8949597.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHA6B_002041"
     CDS             complement(2351..3031)
                     /locus_tag="ACHA6B_002041"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8949598.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHA6B_002042"
     CDS             3157..4026
                     /locus_tag="ACHA6B_002042"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8949599.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHA6B_002043"
     CDS             4052..4567
                     /locus_tag="ACHA6B_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8949600.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHA6B_002044"
     CDS             complement(4614..5177)
                     /locus_tag="ACHA6B_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949601.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHA6B_002045"
     CDS             complement(5232..5411)
                     /locus_tag="ACHA6B_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949602.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHA6B_002046"
     CDS             complement(5488..5997)
                     /locus_tag="ACHA6B_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949603.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHA6B_002047"
     CDS             complement(6093..6473)
                     /locus_tag="ACHA6B_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8949604.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA6B_002048"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA6B_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8949605.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHA6B_002049"
     CDS             complement(7736..9013)
                     /locus_tag="ACHA6B_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949606.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA6B_002050"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA6B_002050"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8949607.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHA6B_002051"
     CDS             complement(9935..10879)
                     /locus_tag="ACHA6B_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8949608.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHA6B_002052"
     CDS             complement(10881..11567)
                     /locus_tag="ACHA6B_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8949609.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3326 a         2467 c         2048 g         3809 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag
//
LOCUS       DAYYHB010000074        11625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000074 DAYYHB010000000
VERSION     DAYYHB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.075"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            428..634
                     /locus_tag="ACHA6B_002053"
     CDS             428..634
                     /locus_tag="ACHA6B_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8949610.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(836..1837)
                     /locus_tag="ACHA6B_002054"
     CDS             complement(836..1837)
                     /locus_tag="ACHA6B_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949611.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1842..3755)
                     /locus_tag="ACHA6B_002055"
     CDS             complement(1842..3755)
                     /locus_tag="ACHA6B_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8949612.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3923..4429)
                     /locus_tag="ACHA6B_002056"
     CDS             complement(3923..4429)
                     /locus_tag="ACHA6B_002056"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8949613.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4589..5029
                     /locus_tag="ACHA6B_002057"
     CDS             4589..5029
                     /locus_tag="ACHA6B_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8949614.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5055..6212)
                     /locus_tag="ACHA6B_002058"
     CDS             complement(5055..6212)
                     /locus_tag="ACHA6B_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949615.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6215..6571)
                     /locus_tag="ACHA6B_002059"
     CDS             complement(6215..6571)
                     /locus_tag="ACHA6B_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949616.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6869..7843)
                     /gene="sgrA"
                     /locus_tag="ACHA6B_002060"
     CDS             complement(6869..7843)
                     /gene="sgrA"
                     /locus_tag="ACHA6B_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8949617.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8039..8878)
                     /locus_tag="ACHA6B_002061"
     CDS             complement(8039..8878)
                     /locus_tag="ACHA6B_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8949618.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9063..9950
                     /locus_tag="ACHA6B_002062"
     CDS             9063..9950
                     /locus_tag="ACHA6B_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8949619.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10544..11239)
                     /locus_tag="ACHA6B_002063"
     CDS             complement(10544..11239)
                     /locus_tag="ACHA6B_002063"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8949620.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11223..11621)
                     /locus_tag="ACHA6B_002064"
     CDS             complement(11223..11621)
                     /locus_tag="ACHA6B_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8949621.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3460 a         2307 c         1738 g         4120 t
ORIGIN      
        1 ttttcttttt tttattattt ttgtttctta ttttctaaaa aattaaaaaa aattttggtg
       61 aattaagtat ttccttgata acactggctt ttggcctcat tttaggttat catttgtaat
      121 tttatttcac agttgatatg attgttatgt aaaataaaaa agtttaggat acatcgaatt
      181 tttggggaaa ttcttttggg ggtatcctaa atcttttcta ttatattccc atattttcta
      241 ttattggtct agacgtatga tggtaaactt tgtacctatc tgattgctga atcattaatt
      301 gcttatcatt agccatcttt gtttcaagtt cgctagatga tcattttatt cacatcctaa
      361 caaacttgtc acatttatag attcttaatc tatttatcat acaattataa aaaaggaggt
      421 gctgcttatg aaagctatcc atatttcttt gctttttttc gctagtgcat tgattttatt
      481 tgctattcat ctttgttccc agccttatat caataacaac ggaatattag tggaaccagc
      541 attcttttgc ttaccactcg gctttttgtc ccttcttgcc tccgcaggct tcggatttgt
      601 tgcttttttt aaacgatcaa aacaacaaat ctgacttata aaatttacgt aaaagagatc
      661 gtgacaataa tcgtgtgata ttcccaaatt tcgaataaaa ggtgcccctt gccatctcta
      721 actcctcagt cacaagttac aatattcata aaccatgaaa agcttgttta ttgaaattgc
      781 tccttgtgac ttggagctaa gtgacagatt acaacctctt ttgcatcata agactttact
      841 ccttgaaaaa cattactaac ttttgatatg tttcttgcgc ttgtttattg ctgaaatcaa
      901 actgatattc atggttcaaa acagcatttt tctccgcata atctactttt gtgactttca
      961 cccccgcttt ctcaaataca gaagttactt cttccatttg tttcgtaaac gtatttgtat
     1021 ttccatccgt taagaatgtg ttttgcgtcc aacgatccgc atgtctgacc aaggctaaac
     1081 ttttgacctt ctctgattgt tcaaagtcct tattatcaaa gtaagcccat ccacattttt
     1141 cataaagaaa gctagagatt ttatcactta cttgagtaag tgcagaagga tccaacagac
     1201 cagacaaaga aataaacttc gtaatctgct tgttctccaa agtcggatca agatgatcag
     1261 cttttttaat ttcttcattt tgaagtgcta acaaatattc tccactaatt tgtgcacctg
     1321 ccgaatttcc accaatcgaa atattcgacc aatctgcttg atcttttatc tcttttttag
     1381 ctgcttgaat ggcttcgttc aattggataa ctggtgtagg atgttttgct tccggtgatc
     1441 gttcataatc tatatttacg atgatttgct gcgtgtctga acatagatat tccaaataat
     1501 cacgacaatc atttttatcc cctcctacat atgcgcctcc atgcatccag aaaagaatcg
     1561 gtaatttttt cttttccact ttcggtgtgt agatatccat attattattt ttgtactttg
     1621 aaggatagga tatttctttt tttatatcga tttgttcgat tttttgctgg aaatcatctg
     1681 gtcgggcata tacttgttct ttttcacttg tgtcaaattg ttttctaaca agcaagctaa
     1741 tcggctttgg tgtgatttga ttaatcaaaa atacagtgac taaaataact acgatgataa
     1801 ctggtactgc aatcactatt cgcttgaacc atttcattca tttaccctct ctttcttcgc
     1861 caaatccatg tgataatcgt gctaacagat aaaaatccac ctgaataaat gagaagcgaa
     1921 tgtttctttt ctccagtttt cggcaacatt cttttttttg catctgtctt tttttgtact
     1981 ttcactgtca tctgaatcgg ttgcgtatca tcacttgatt gatcctctcg aggttcattc
     2041 tttcccttat tgtctgcatt ctctgtcgga tcttgctttt ttggtggagc tactgggatt
     2101 tttttgtaat agaaaataat agttggtatc gtctcattaa aaatcccttc agtcggtcct
     2161 tcaacatttg ctaattcgta tccttcgaat gtcttttgct ctgtcatata attttctcca
     2221 taagctccgt aaagctggat ttcgtcctgg tctttgagta gacttttatt gttttcatcc
     2281 acatacttta cgtttatctg atcatggaac actactttca aaaagtccgt tacatgtttc
     2341 gtgtaggcag catggtcaac ttcctgtgcc tttccatgcc ctgctccttc tactaataaa
     2401 agatctttat accctcctga taactcatat aatttttgcg cgttagacac aggtactaca
     2461 tcatctgctg ttccatggat aatcaattta ggcaatgaat ctgacattcc aatcgtaggt
     2521 agagcagctt ttgtatccaa atgcatctcc tgatcaaaat agtattgttt caaaaggccc
     2581 ataaataaat cttcatgctc ttgatcccca atgatgtcta actgattttt tatggcatcc
     2641 ggtagtgcgg gggctactgt ctggctgtac gtaaaacgta gttgttcatc aattgatgca
     2701 aatccgcaat tttcaataat tcctttgaca ttgacaggaa tatctttcgc taatacagac
     2761 atcatcgtag ctgctcccat cgaaccacca taaagtaaaa tcttttgaga tggccgctct
     2821 cgaacttctt gatttatcca gtaaaggacg tcatctgact catattgtcc ataggtcaca
     2881 taatcgcctc cgctattacc agtcgctctg gaatcaacaa ataataaatt atacccggcc
     2941 ttataaaaat cattgtattc ttctgtaacg atcccgttat tccaattccc tcgaaatccg
     3001 ccatggatca tgactgtttt atctgaacct tgatcgacat aatatgcata tacatccatc
     3061 attttgccat cttgtttgtt tagtacagtt tgatgaagtt gttttgtatt tggatagaag
     3121 cctgtgtttt ctgtaggcgt tgtcccatca tctgtacctc caagcatcga catcaacaca
     3181 ctgttttttg caaaagcgat atcgtacaat agataagtag caatcagatg tttgataatc
     3241 tctttatcta caccataatc attaacagca agctcagtga ctaataactc tattaatttt
     3301 gacttgatgt cttctgttgg tccattaagt atttctgtca tctgtttacc gaagtaattc
     3361 aaatattctt taccattgaa attttcacct ttaccgatat catcattttc tgtcccattc
     3421 tctttgtcac ttactttagc agaagaggat tcgtcgtttg acatttttgt agctgttgct
     3481 tcttctgttt gttggttatc tgtggtagcg gtcgtttctt gatctgtgct agtaacagat
     3541 gtctcaaccg ttgtttcagt atttgtttct gtttgtgaag tactcgattt cgtttcattt
     3601 ccagcatcag tagtttctgt ggtttctacc tcccgagatt ccgattcgga agaaatgctc
     3661 tctccatata ctgtccattg cgggacgctt gtacttacag caaataagat gaccaacgct
     3721 ccctttaata cttgttttgt tctcaagcta ttcactcctt tttatgtttc taggagaatt
     3781 atatgtaaac gcaaacattt tttatacaaa aaataaatgg tttatcccat aaaaatagaa
     3841 caggattatt ttctcatttt acgtaataaa gcagaatgga taaaccagta tccattctgc
     3901 tttttttaaa cgaagaaaca acttattgct ccaaaaatgt cagaaatcgt ttatcccaaa
     3961 ttttctcgta aaaagcgatt gtttcctttg ctttcataaa aggattatca taagttgtcg
     4021 ttgctcctct acacatcata agctggtatc ctaatccatg agcaaacttc acagtcgtat
     4081 ctatacaata ctctgcttgt gccccacata attcgatcga atgacagtcc aattcagtta
     4141 ataaaacact caaattagtc ttataaaatg aattcgcatg ggtttttctc acaaaataac
     4201 tgtgctcagg aatagataat tccgacaata gctcccatgg ttttgtttgt ggcactaatt
     4261 gagcatcttc gtgttgaacg aaaataactg gtttcccaat cgtttcgtat gttttgattc
     4321 ttttattcac taatgctgtc actgtttcta attcgtgtat cggcgaagaa tcaaaacata
     4381 cccctttttg taaatcgatc actactattg cgtcggctgt gtttgacata cttgtgcctt
     4441 ctttcttttt tcttgattta atagtatttc ttatctagct agttccatta tacgtgacaa
     4501 cattttctta ttttacacct ctaaatggca cttttcgact atttcttcat gttattattt
     4561 cataaatact tagctattgg aggaatgtat gatgattaaa ttgtatacag gttctggctg
     4621 ctcgtcttca agacgcgcaa aacgttggct tatcgtccat gatataccgt ttatcgaaca
     4681 gaacttaatt cgtcaaccac taaccaaaaa tgaatttatc cacttactaa aattgacaaa
     4741 caacggcgtg accgaaatgc tctctactca ttctattagt tataaaaatc ttgttcacag
     4801 aatagatgaa ctcagtttga gtgaactata tattgagatt cgtacatccc cttctttatt
     4861 gaaaaaaccg attatctacg accaatctgc aaaacttttg actggattta gcgaagataa
     4921 aatcaggatg ttcctttcta aaactaaaag aaagcaagag ctcaacgaac tcttgctttc
     4981 tggtcatctt gtcgatttta aagaaaataa tctttttgca atgtcttaat tttggcataa
     5041 acaatctgct tttcttaatt aacgaaatcc aatttttcca atatggatct atttttctca
     5101 ttcctgattt ttatgatttc agctttcaga cgcgcccaat cattttttgt aggttgtcca
     5161 ttccagtaaa atatctgtgt attcgtttga tcatatgcct ttttgtcgat aggataatcg
     5221 ctaatgacaa tatcaggtaa ctgatcagcg gatgcactat attttattgg gcgttcagtc
     5281 atagataata tccttttctt tgcccataat tcctgtgttt taccatactt tgattgaata
     5341 tagaatatga caggttgttc gtgttttata aaaatatccc tgactaacat acagtattga
     5401 aaaatcaacc gtctgttctt ttcataataa gtattaagct ctggaattct gcgagctacc
     5461 ccactaaaaa actgttgcat gattggatac atcactggaa atatatgctg aaaatctctg
     5521 tcttctgctg attttccaaa agcatccacc ttcttttttg tcccaaaaac ccttgaatac
     5581 aaatggatca ttgccaaatt gacatataga taattttttt ctgaagttgt aaaacaaatc
     5641 ttgaagtttt tttcaaaatt ttctacaata gacgattcaa tccagcccca actttcttgt
     5701 actttatctg gagtatccaa caaatcttct tccaaatagg tgttcattac accaaacata
     5761 aaataaaggt aagcttgctc tgttggagtg atgtgattca tcgaccagaa gtttgcttca
     5821 aaaaaaggct ctagtatgta atgcgtaaac tcttcttttg ttatcaatgg atgcacaaat
     5881 ttgttcatct cttctgggac atacgtaatc gagtggcctt gtttcattcg ttgcaacgaa
     5941 attcttagta ggctgatcca tttctttcta tctatactac gcgtatatgg cgcatgtttc
     6001 gcaaattttt ctaatagttg acgtttattt tttatacttg ataatatatc agaatgattt
     6061 tcgtcgaatg actgccaata catcaagtga aaaaagtatc ggatacggta ttcttctcct
     6121 tctatcggat tacctttttt caagctgcaa gataaatgat agtttttcaa aatagtatta
     6181 agttctctta attttctttg aatcgtacgc aaacttatat aattttttga ctaagatttt
     6241 ctaaggattg ataagtacct ttgtaaagtg aatccagtaa aatataacca atcgttccct
     6301 ctaaaagtat agaataaact ttactcagta aaaactgaga agaaaactcg acttttatca
     6361 tcttgttttc tgaaagaaaa gataatttat attctggata cctttcgcaa aacgattctg
     6421 cttgttgaat caccgaattc atcttagcca gggtgtagtt tagatctaaa gcgatatcct
     6481 tcaccacata taatttctcg tctctttcat aacaatagtt caaaaattga tatacttgcc
     6541 tttcattgat atcaataaat tcttcaaaca aagtacatac tcctttttat ctattattta
     6601 gacttattcc gacagctaca tgaataacgt acaaatagat tcgttactga tttttcagag
     6661 atactaccat gcgcagtgtg cattgtcaat tattcttgct cttattttcc atttgtgttc
     6721 ctgttatttc ctgtttctca aatatgttag taaacaaaaa tagatctggc aaagctacaa
     6781 cttcgccagg caatacagca ctctcgaatg aaatattcac tcaaaaatct atcgttttat
     6841 tcaattttag atctgttgct tttattcttt atttcactcg atggaagaga gaacgaaaat
     6901 agcttgctcc tacaatcaaa atgagtaaca cacccatcca tgtaattagc ttgttcaagg
     6961 ttctactacc agtttctggt aaatcgcttt ttctattatc tgtttctttc gctttttctt
     7021 gatgatcaat tccacgagaa acaaaatcag gctgttttag cacacttttt aggttgctcc
     7081 ctgcatttct cttttgttcc ttagttggta tgataggtac caaatcaagt tcattattta
     7141 cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc
     7201 ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg cttcctgtgc
     7261 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgttttct gtgctacttt
     7321 cactgctcgt actctctgta ctgctttcac cgctcgtgtt ttctgtactg ctttcactac
     7381 ttgtgctatc cgtactgctt ggcggtccaa caactgtcca actgcttggt ttcaaattct
     7441 ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc aactttatct ttactcttga
     7501 tctcatcaat cgatagttct gcagaaatca ttattgtata ctcaatacca gcggtccatt
     7561 gtacgtcttt taaataaact tccccaataa ctttttctga acttccatta ccatcccaac
     7621 catatgttag aacagcatgt acatcactag cattcacgct tttgtttggt ttgactggaa
     7681 tagtaatgac ttgttgtgtc atgtcataaa caatccgatc agttttcaca gtaaatgaaa
     7741 gattctcatt tgcccgttca tttgcagaag tactgattcc aatcattgct gcaattatac
     7801 tcagcccaag aataacaaat aatacggtcg ctgttttctt catttctatc actctccata
     7861 taattacctt ctatttgata aaggaattat ataaaatgaa aatgatgaga acacgacagt
     7921 cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt gaatttgttt actttttcgt
     7981 ttaatcaatg acagacttaa aaaagcaaga ccgatatcag ccttgctttc ttcatctttt
     8041 attgataaac agcagtgtat ttcttcatcc gctttgtttt atatgcatag agtaacaaac
     8101 caacaatcaa gactaacaag aataatgtag ttgatataga tgcttcagcc ccaaagtttc
     8161 ccccagtaat tattgtattt tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg
     8221 ttccgctgac tggtataccc aaaatagctc cttgtgtccg attccaagta atatgagcac
     8281 caatagccgt aaataaactt cctgtactgt agataagtgc gctgaaaaag caacctgcta
     8341 aaaagatatt gaaaactgcc agaaatgtca tgttaggatt caatccgtgc aaaagagcaa
     8401 ataatgcagc agatccaatc attgctatta acggaccaaa tctaagcatc tgatgctgaa
     8461 aaaagccacg gaataaaagc tcttctgatg cccccactgc aataaataaa ataaaactcg
     8521 ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg atacgccccc gtcaaatata
     8581 atggactagt aataagcaga aacactccaa ttccaacaac gagacctccc gctaaatacc
     8641 cccagtcttt tcctcgaaca gaaacaccat aatcttctat tctttttcct tcgaccttac
     8701 tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc
     8761 caatccattc actatttcca ataaacatat ccgcaagtgc attattgaca agaattccta
     8821 aaacaaaacc aactaagact attaaaacag atccaatcaa ccgaataatc ctgatcatct
     8881 ttcatccctc ctttatatta agtttaacaa cccttttctt tcttgtctcg tgaaaactat
     8941 gtaaatgttc tccatcatag agagaataga tttttacgaa ctaagaatat ctacagcaac
     9001 cttacgaaaa tgaaagactg tctccctaat tcttctgtta gactgttttt aataaggaaa
     9061 ggatgggaaa ttttttgaaa aaacaactac taattatgtg cctttcagca tgtgcaattt
     9121 tactattctc aggttgtagc aagtccaatg aaacgattgc aacgtttaaa ggtggaacac
     9181 ttacagttca ggatttttat gataacccaa aagttaaatc aaataatcaa acactattaa
     9241 aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt tcttcaaagg
     9301 aagtaacaaa agaatataat gaacaaatca aaaaactcgg tccaaactac aaggaacaat
     9361 taaaagcagt tggccaaaca gaagagacat ataagaaact ctttaaacag atgctggctt
     9421 tccaatacgg tttgaaagcc aatgtaaaat taacagataa agatctagac actgcttgga
     9481 aagaattcta tccagaagtt agtacacaga tcattctatt ttcgactaaa gaaggagcag
     9541 ataaagcaaa aaaagaagca aatgaaggag aaaatttctc caagcttgtt caagcttatg
     9601 gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa ttttgattca accaatccag
     9661 agattcctac tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa gtttctgata
     9721 tcattcctgt aacagatcca acgacttatc agcaaagcta ttatctagta aaaatggtaa
     9781 aaaaacagga taaaggttct aataaagaca aatacaaatc tgagctagaa aaaattgcta
     9841 ccgaagcaaa aactcttgat acggaattca tggataaaac aatccgtaaa gtgatgaaaa
     9901 aagacaatgt aactattaaa gatccttatg taaaaaatat attcaaataa acaaatttga
     9961 cccaattggg gatgtggcaa aatttgagaa gtgatttttg gaaattcctt cttatttttg
    10021 aggcaaacca cttttgccac aacttcatcg gggccttttt ttatttgtac caaaacaaat
    10081 cactggattt gttacaatca tgaggatata atttaaaaaa gttttttatt tctatcacat
    10141 gaatcaatgc gccattaagt agattctttc atgcttccct attttttcac ttgccgtatc
    10201 gcttcaaaaa caactatcat aaactcaaac tcgactgaaa agttatttta aaaaagacta
    10261 aaaatatccc ttctggactt gttcttctta ttctgttata ctagagaaac acgaaagata
    10321 tttttatgcg acacttttca tttgttttta tgaaatttat tcaaaactgg cgtaacttca
    10381 gagttacaag gatgtattag aggttaggga atacatcgtt taaatgagaa ataagtaata
    10441 gttagtaata gtcacccaaa ctttaaatta atgaatattg aatcgtcaga tagcctcctc
    10501 gagttttcga gaaggctatc gtttatttta tggagaaatt gattcagaag taatcagcga
    10561 ccttttttag ttcaccaatc gtttctgctt ggaaagtttg cacccaatct tcttggaagc
    10621 ccctcactgc tttatcaatc gagggcatct ctaatacttg atctagtagc tctttaccta
    10681 acgttatcgc atgagctcca gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac
    10741 tagctgctaa tatttttgtt ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa
    10801 tcactttcat agcatcaatg ccttgatttt gcatcctgtt ataataaggt gcaatataat
    10861 ctgctccatt attaatagct aaataccctt gtaattctgt gtaaatagct gtaccggtaa
    10921 tcaaataacc ttcttttttc aactttttga tcgcttgaag gcctgcttga ttcaccggca
    10981 ccttgataaa cgtatctttt cccagttctt ctatgataac tcgagcatcc tctatcattt
    11041 cttcttccgt tcttcctatt gtctgaatat ggataggaat ctgtccgatt atttttttga
    11101 tttctttcat atgcgagaag aagtttgacg ctccttcttt tttcagaatt gtaggatttg
    11161 ttgttacgcc tgttaaattc aaaaactgtt gacaagaata aatacttttt gtatttgctg
    11221 tatcaagcat gatttccata tagctttccc ccattctgat cctcttgatc gattttaatg
    11281 attgtcccct ttcttaaatg cggcatttcc ttatcttcta catacattgt tcctggaagt
    11341 tccggctcat gagatccatc aaattttatc gtgatatgtc ctaattcatt caaattagtt
    11401 tggacaacat ctcctactgc tgtgattaca tacatttgtt tatcaaaaca gagcttcata
    11461 tcacaagtta tttcttttct tacctgattc aattcaatct tataacaata atctgttaat
    11521 gtttccggtg cttcatctcc aaataaaatc attatttttt ctttcctgaa tacttctgct
    11581 tctataccaa catctgttac aaccgtttca aagatcgtca tctct
//
LOCUS       DAYYHB010000075        11547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000075 DAYYHB010000000
VERSION     DAYYHB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.076"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..728)
                     /locus_tag="ACHA6B_002065"
     CDS             complement(243..728)
                     /locus_tag="ACHA6B_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8949622.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    818..1163
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1208..1849
                     /locus_tag="ACHA6B_002066"
     CDS             1208..1849
                     /locus_tag="ACHA6B_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8949623.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1868..2503
                     /locus_tag="ACHA6B_002067"
     CDS             1868..2503
                     /locus_tag="ACHA6B_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8949624.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2521..3336
                     /locus_tag="ACHA6B_002068"
     CDS             2521..3336
                     /locus_tag="ACHA6B_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8949625.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3655..5091
                     /locus_tag="ACHA6B_002069"
     CDS             3655..5091
                     /locus_tag="ACHA6B_002069"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8949626.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5280..7727
                     /locus_tag="ACHA6B_002070"
     CDS             5280..7727
                     /locus_tag="ACHA6B_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8949627.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7957..9024
                     /gene="rlmN"
                     /locus_tag="ACHA6B_002071"
     CDS             7957..9024
                     /gene="rlmN"
                     /locus_tag="ACHA6B_002071"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8949628.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9014..9220
                     /locus_tag="ACHA6B_002072"
     CDS             9014..9220
                     /locus_tag="ACHA6B_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949629.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9217..10320)
                     /locus_tag="ACHA6B_002073"
     CDS             complement(9217..10320)
                     /locus_tag="ACHA6B_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8949630.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10873..11421
                     /gene="aac(6')"
                     /locus_tag="ACHA6B_002074"
     CDS             10873..11421
                     /gene="aac(6')"
                     /locus_tag="ACHA6B_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8949631.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3838 a         1950 c         2487 g         3272 t
ORIGIN      
        1 actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat
       61 gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta
      121 ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat
      181 atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat
      241 cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg
      301 gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga
      361 cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct
      421 aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca
      481 aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca
      541 tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt
      601 acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg
      661 actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca
      721 cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa
      781 tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt
      841 agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga
      901 aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac
      961 agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa
     1021 aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc
     1081 atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga
     1141 accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga
     1201 aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac
     1261 attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt
     1321 aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat
     1381 gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg
     1441 gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta
     1501 ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc
     1561 gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac
     1621 aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt
     1681 gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga
     1741 cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct
     1801 aacaatcgta atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg
     1861 aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa
     1921 agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg
     1981 cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt
     2041 ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg
     2101 agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt
     2161 agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt
     2221 agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg
     2281 acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta
     2341 tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct
     2401 atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga
     2461 aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag
     2521 atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt
     2581 tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt
     2641 ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca
     2701 ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt
     2761 cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata
     2821 tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat
     2881 atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa
     2941 atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa
     3001 caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt
     3061 aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta
     3121 tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt
     3181 tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa
     3241 aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat
     3301 aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga
     3361 cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg
     3421 agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta
     3481 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
     3541 gaaactaagc gactttttca caacctcctt tacatatttt tattttgttg tggaaggggt
     3601 ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa
     3661 cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat
     3721 caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta
     3781 cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat
     3841 cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct
     3901 gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct
     3961 ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta
     4021 tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag
     4081 atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc
     4141 cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca
     4201 gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat
     4261 ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga
     4321 ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct
     4381 gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa
     4441 tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct
     4501 ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa
     4561 acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca
     4621 ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat
     4681 ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga
     4741 ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga
     4801 gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat caattactta
     4861 caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat
     4921 tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga
     4981 tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac
     5041 agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac
     5101 ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa
     5161 attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa
     5221 aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa
     5281 tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa
     5341 aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg
     5401 tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca
     5461 ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg
     5521 tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct
     5581 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
     5641 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
     5701 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
     5761 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
     5821 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
     5881 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
     5941 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
     6001 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
     6061 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
     6121 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
     6181 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
     6241 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
     6301 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
     6361 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
     6421 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
     6481 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
     6541 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     6601 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     6661 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     6721 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     6781 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     6841 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     6901 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     6961 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     7021 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     7081 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     7141 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     7201 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     7261 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     7321 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     7381 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     7441 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
     7501 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
     7561 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
     7621 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
     7681 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
     7741 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
     7801 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
     7861 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
     7921 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
     7981 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
     8041 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
     8101 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
     8161 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
     8221 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
     8281 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
     8341 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
     8401 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
     8461 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
     8521 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
     8581 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
     8641 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
     8701 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
     8761 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
     8821 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
     8881 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
     8941 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
     9001 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
     9061 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
     9121 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
     9181 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
     9241 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
     9301 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
     9361 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
     9421 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
     9481 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
     9541 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
     9601 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
     9661 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
     9721 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
     9781 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
     9841 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
     9901 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
     9961 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
    10021 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
    10081 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
    10141 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
    10201 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
    10261 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
    10321 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
    10381 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
    10441 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
    10501 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
    10561 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
    10621 tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt
    10681 tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa
    10741 tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
    10801 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
    10861 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
    10921 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
    10981 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
    11041 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
    11101 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
    11161 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
    11221 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
    11281 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
    11341 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
    11401 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
    11461 taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga
    11521 agaagctact tttggaacac cgtgtac
//
LOCUS       DAYYHB010000076        11546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000076 DAYYHB010000000
VERSION     DAYYHB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.077"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..569)
                     /locus_tag="ACHA6B_002075"
     CDS             complement(<1..569)
                     /locus_tag="ACHA6B_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949632.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKN"
     gene            complement(580..1248)
                     /locus_tag="ACHA6B_002076"
     CDS             complement(580..1248)
                     /locus_tag="ACHA6B_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8949633.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1378..1962)
                     /locus_tag="ACHA6B_002077"
     CDS             complement(1378..1962)
                     /locus_tag="ACHA6B_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8949634.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2293..2526)
                     /locus_tag="ACHA6B_002078"
     CDS             complement(2293..2526)
                     /locus_tag="ACHA6B_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8949635.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2541..3491)
                     /locus_tag="ACHA6B_002079"
     CDS             complement(2541..3491)
                     /locus_tag="ACHA6B_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8949636.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3488..4330)
                     /locus_tag="ACHA6B_002080"
     CDS             complement(3488..4330)
                     /locus_tag="ACHA6B_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8949637.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4332..5051)
                     /locus_tag="ACHA6B_002081"
     CDS             complement(4332..5051)
                     /locus_tag="ACHA6B_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8949638.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5599..5901
                     /locus_tag="ACHA6B_002082"
     CDS             5599..5901
                     /locus_tag="ACHA6B_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949639.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            5973..7199
                     /locus_tag="ACHA6B_002083"
     CDS             5973..7199
                     /locus_tag="ACHA6B_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949640.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7788..9236)
                     /locus_tag="ACHA6B_002084"
     CDS             complement(7788..9236)
                     /locus_tag="ACHA6B_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8949641.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPDEYVLAINSMTKPNPTFKKEMKSIKNARGEDDILFII
                     DVEPSISHVIFNSNGDCFLRIGDKSVHQTHDQISKLEYDKGQRMFEDIEILDSTLDDI
                     DESLMDSYKKLKKAEAISTETILTGRGLMRNGHLTNAGMLLFGKNTFLALPNSRIRFL
                     RYDGKRNETGRRLNIVKEFDYEGAIPKLIESITRDVKTQLREFQYLNDDGKFSIIPEY
                     PEFAWFEGIVNALTHRDYSYKGDYVRINMYDDRLEIFSPGALPNIVTLQNMRRTRYAR
                     NPRIARTLSEFGWVKELNEGVNRIYDEMQLLFLNEPKYSEPNNNSVLLVLENSITSRH
                     LRERDRLDDYLGEFEVDSLSTSEKMIVSHLFNNEKISVKEASEVLKKGVGYSRKVLKE
                     MLRKKIIQWHGTSTKDPTQYYSLNRVKQSKIE"
     gene            complement(9382..10881)
                     /locus_tag="ACHA6B_002085"
     CDS             complement(9382..10881)
                     /locus_tag="ACHA6B_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFM8949642.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKSFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYYCIHSDI
                     TDCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLM
                     DFVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLS
                     DLNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVK
                     LLGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVI
                     LGKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKV
                     SNPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(11053..11298)
                     /locus_tag="ACHA6B_002086"
     CDS             complement(11053..11298)
                     /locus_tag="ACHA6B_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8949643.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
BASE COUNT         3856 a         2074 c         1520 g         4096 t
ORIGIN      
        1 ttatttttta aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt
       61 gaagacggtt catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa
      121 attttctgcc gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga
      181 tctaataatt cagctaattc attgattctt ttatccatat gctcttttgt aaatccaaag
      241 ttttccatcg aaaatactaa ctcactcgtt gtatttgttg taaagaattg tcctctagga
      301 tcttgactta cgtagccaat attttttgat atactttctt gtgttaagga ttgtagcgag
      361 ttattttcaa ataaaatttc cccaaccaat tttccgtcat aaagtcttgg aataagtcca
      421 ttcattaact ttaatagtgt ggatttccca ctcccagaag aaccagtaat caatgtaaga
      481 ctgccttttt ttattgaaaa attaaggtca acaagcgctt gttccttaac atccttatat
      541 gaaaaagaaa cattgttaaa ttcaaacaat tctttcacct caaatcaaaa tacttaagaa
      601 aaaaatagta acggaaaaca ggatgatata gtctaataag ctcatgtttg ttttatagta
      661 agaacttctt ttcacaggag attctatccc ctttgttatt gcattagcgg tcatttcttc
      721 agcaatcata gttgcacgta taagaacggg aactaacaga aattccaaat attgcagagg
      781 ctgaaaaaaa gacaagcgaa tgtttctcat ttttaaagat tctttaatca ttcttatttc
      841 agaacgataa gtcggaaaga atctgatcac tactgtaata cttaacatta tttttttcgg
      901 taaatgtatt ttttctaaag aggataataa ttcattgcta gtatagacac taaccaacaa
      961 ccatgagatc attacgaccg gcaacattcg aaatacaata tataaggatg tcgcaaaaaa
     1021 tatagtaaaa aactctatct tgatagcatc aataattttc attactaaaa acagaactga
     1081 ataaatcaag atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt
     1141 agcaataatg aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg
     1201 aaaaaaaata tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc
     1261 aaaaaataag atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca
     1321 gtgcgatctt atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta
     1381 actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat
     1441 agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta
     1501 aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt
     1561 ctttaactta tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac
     1621 aatatcttga tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt
     1681 ctcaaaaata taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc
     1741 tatatctata ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat
     1801 aagcgcatta atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg
     1861 tttataaaaa gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg
     1921 ctgaaatcct tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat
     1981 aatataatct ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc
     2041 ttacacagga cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct
     2101 aaactaatga gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga
     2161 ctcatctaaa tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc
     2221 cagctattag gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa
     2281 aaataaagtc ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc
     2341 agttaattga aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact
     2401 tacagatgct ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt
     2461 tctttttgat aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg
     2521 taccatacct accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat
     2581 ttcatcatcg catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa
     2641 atcgttgaat agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc
     2701 acactgcttt ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt
     2761 tttatttgat ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc
     2821 acattatacg ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta
     2881 tcattcggaa ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca
     2941 atatacttat ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac
     3001 tgtttttcaa tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga
     3061 tcttccttcc ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct
     3121 gcaaaatagt ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca
     3181 tttcctccag tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt
     3241 tgaacatctt caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg
     3301 tgtaaatcaa ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta
     3361 actactactt ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa
     3421 catgccccga atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat
     3481 tttctcatca taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa
     3541 aactaattac aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa
     3601 aactataacc aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa
     3661 atatcatagt tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca
     3721 ttgcaacaat taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata
     3781 gaaccatcaa tagataatga taaaaactga cattcatccc tattgctttt gccattaatg
     3841 gatcaaaaga agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa
     3901 ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata
     3961 aaatatgaaa taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta
     4021 aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta
     4081 taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa
     4141 tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca
     4201 taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa
     4261 ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat
     4321 tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa
     4381 ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
     4441 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt
     4501 tttccctgat gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct
     4561 atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct
     4621 aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt
     4681 cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt
     4741 tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac ttttattgga
     4801 aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg
     4861 atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta
     4921 agcaatgtgg attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt
     4981 atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc
     5041 acttctaaca taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca
     5101 aaaattatat tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga
     5161 ttagaatgac aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa
     5221 aaaggagcgt tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa
     5281 ttatacttga agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa
     5341 ttattcaaga ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact
     5401 tttttcgtag tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa
     5461 ctatttttga tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc
     5521 tagaaactca agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt
     5581 ctgggaaaac aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc
     5641 gtaaactggg gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata
     5701 actactattt taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc
     5761 tgtgcatttc taggaatcga taataatact ctagataagt ggattcgtat atataatttc
     5821 ccagtaataa gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg
     5881 aatacattta aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata
     5941 tactgcctta ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat
     6001 ataaaactaa tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa
     6061 agtatcaaaa aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga
     6121 agaaaataga aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa
     6181 taacgtttaa aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa
     6241 ttagatcaaa caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt
     6301 tgcatatttt acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga
     6361 taatatcaga tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag
     6421 gatatgttcg ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa
     6481 ctactaaaat tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa
     6541 tacaagaagg taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa
     6601 atgatgattt taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc
     6661 tgtatcttgg tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg
     6721 aaaaacaaac ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa
     6781 aaggtcgaaa agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat
     6841 ggaaatcggt acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt
     6901 atctctttac ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc
     6961 taaactatcg aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac
     7021 acaaactccg tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa
     7081 tttcaaatgc tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag
     7141 atatagttga taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat
     7201 atgtgttctt aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg
     7261 ccagaaacgt tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct
     7321 ttcttaagac ctggttaaac aaacttttca tttataatgt tttacagaga tatatgtcgt
     7381 atgaaattaa tgttctcttg ttcatgaagt tttacagaat tgtatagggt tatctgttgt
     7441 cattacctca aatcattacc acaacttggc gagttggctg attgaagaag tactctgttt
     7501 tactctatct tactctgctt tactctatct tactctgctt tactctagct tactctgctt
     7561 tactctagct tactctatct tactctatct tactctgctt tactctatct tactctgctt
     7621 tactctagct tactctgctt tactctatct tacctgcttt actctatctt acctgcttta
     7681 ctctatctta ctctgcttta ctctatctta ctctgcttta ctctatctta ctctgcttta
     7741 ctctatctta ctctatctta ctctgtctta ctctatctta ctctgtctta ctctatctta
     7801 ctctgtttta ctctatttaa agaatagtat tgagtcggat ctttggtact agtaccatgc
     7861 cactgaatta tttttttcct taacatttcc tttaatacct tacggctata tccgacccct
     7921 ttttttaata cttctgaagc ttcttttacc gaaatttttt cgttattaaa taaatgtgaa
     7981 acaatcatct tctcagaagt agacaaagag tctacttcaa attctccaag ataatcatct
     8041 agtctgtctc tctctcttaa atgtctagag gttatactat tttctaagac taataaaacc
     8101 gaattattat ttggctcact atatttaggt tcatttaaaa atagtaattg catttcgtca
     8161 tatattcgat tcacaccttc attaagttcc ttaacccatc cgaattcaga taatgtccta
     8221 gcgattctgg gattcctagc atacctagtt cggcgcatat tttgtaaagt gactatatta
     8281 ggtaaagctc ccgggctaaa aatttctaac ctatcatcat acatattaat tctaacgtaa
     8341 tcccctttat aagaatagtc tctatgcgtt aaagcattga ctataccttc aaaccatgca
     8401 aattcaggat attcaggtat gatactaaac tttccatcat catttaaata ctgaaattct
     8461 cttagttgcg tttttacatc cctagttata gattcgatta gctttggaat tgcaccttca
     8521 tagtcaaatt ctttaactat atttaatctc cgtccagttt cattacgctt accatcatat
     8581 ctcaaaaaac gaattctaga attaggtaag gctagaaatg tattcttacc aaatagaagc
     8641 atgccagcat ttgtcaagtg accatttctc attaaccctc ttccagtgag tatggtttct
     8701 gtggatatcg cttcagcttt ttttaatttt ttatatgaat ccatgagaga ttcatcaata
     8761 tcatcaagag tactgtctaa aatttctata tcttcaaaca ttctctgtcc tttgtcgtat
     8821 tctagtttgg atatttggtc atgagtttga tgaacacttt tatccccaat tctcaagaaa
     8881 caatcaccgt tcgaattaaa aatcacatgg cttatgctag gttcaacgtc tataatgaat
     8941 aatatatcgt cttctcctct agcattctta atactcttca tttctttttt aaaagttgga
     9001 ttgggttttg tcattgaatt tatagctagt acatattcat ctggatcgat cgcaccttga
     9061 aatttaaacc cagaaatagt cccatcatct tctataccga ttaccagctt acctccatcg
     9121 gcatttgcaa acgctactat gtgttttata atatctctag gcttaatccg tgcacttttt
     9181 ctatcaaaat actgatcttc tttttcactt ataaactttt gaatctcata atccatttaa
     9241 aatttccctt cttaaatttt tttattatag agccgtatat tcaatttcca caaaaaaata
     9301 atctaaaaca aaacagacac cttcattttg atatgttctc ccacatatga atcggtcgct
     9361 atacagcatt acatatagta attattcata ctgcgaaata aatagctcca aatctttagc
     9421 tggcgtagtt aagcttaaat tcgaaatgaa agtttcattg actattccac tttcatcaaa
     9481 tcccttcttc aaccaatcag agttccacaa cgtaaatcct ttaggattag aaactttctt
     9541 acataatttt gcactatagg atttctctct tgaatgaatt aaagaaagtc tttgtaacca
     9601 aatttcaact atatccgtat taggagtatt aacaaatttt cttaagattt tatccaagta
     9661 taagttgatt tcttctacag caataaactc aaaaagctta cctaaaatca caatacaagc
     9721 ctccaaagtt ctaggattct tttgcattat attaacaaga atactaatca attggtagat
     9781 atcatctggt cttttgttta aagtagaaac tctatgtgta tagacttcac ttagcgcttt
     9841 acttaaactc ccgcaatttg gatatttgtc tcctaaaagc tttacctgta gcaaatgctt
     9901 ttgaaggcta agcttaaact tccacttatt attacttttc tctgtaaaac ttgcatgttt
     9961 aagatcccag taaattttat caggcttaat tgcatctaaa attatattat ccgataagaa
    10021 tgttttattt gaatttagct tcaaatttag atccgataat acttcagata acaatctgat
    10081 aatcacttca gcttgatctt ttcgaaccga aaaaattcta taatcatctc tgtaacgaag
    10141 tattttgaac tctttaatct ctgttttttc tatcttgttc agtaacattt tatcagcata
    10201 cccaagtact atttcagcta caaaatccat caatacattt ccttgaggta taccgttcgt
    10261 ttgtccattt tgtaaatatc gtattttaga atctattaag tttccaacgc cttgagtatc
    10321 ttttctatgt gtcttagccc attcttttga atgtactgcc cacgggatag tatgtgtgta
    10381 cattgagcta taacaatcgg taatatctga atgaatgcag tagtagtcat actccaaagc
    10441 ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt ccgcagtatc
    10501 gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct tgtcggcttg
    10561 gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa tcccgtttac
    10621 taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat aattaccatc
    10681 tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc tcttatgtct
    10741 aactagttca gtaaatgatt tgttttttaa taaatccact gctttctcaa tgcctactga
    10801 taaatcaata taattgggca acgatgtact aaaaaaactt gatgttttta aaaagaattg
    10861 ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca aattcaaaat
    10921 ctcctagatc aattaagttg taatgtaaga ggatacccta acgttacata tctcattcat
    10981 tattatacca gtatttcttc atgacacata agggtgtaac ataataattt taaacgaaat
    11041 gactacaagt agtcattgaa gtaagtcttc ataagatacc tgtaggatat ccctaattgc
    11101 tctcaattga tctaacttaa tgtgttgccg gccaccttca attttaacca atgtttccct
    11161 agttatagca attttatgaa gctgtaggtc tctaatcagc tcggtttgac caattccttt
    11221 ttctaatcgg attcgtctaa tattgctacc gattacattt tctttatcaa taattttttc
    11281 ctctttcaag aatagcacac cttttggact aaaattagtc ctttttctta ttaatcttaa
    11341 agattcggta tgctacaatg ggactagtat tagtccacat tcaattttaa ggaggaattt
    11401 ttttgaagaa atttgcaatc attctaacct gcgctttact catcggaact ataagcgggt
    11461 gtaccacttc tgaaacaagt tctacaagta cggaaactag tacaacaacc tcagaaaaga
    11521 attacccaat acattttttt ccggtt
//
LOCUS       DAYYHB010000077        11514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000077 DAYYHB010000000
VERSION     DAYYHB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.078"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACHA6B_002087"
     CDS             complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACHA6B_002087"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8949644.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACHA6B_002088"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACHA6B_002088"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8949645.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACHA6B_002089"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACHA6B_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8949646.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACHA6B_002090"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACHA6B_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8949647.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ACHA6B_002091"
     CDS             complement(5326..5721)
                     /locus_tag="ACHA6B_002091"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8949648.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACHA6B_002092"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACHA6B_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8949649.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ACHA6B_002093"
     CDS             complement(6198..8390)
                     /locus_tag="ACHA6B_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8949650.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ACHA6B_002094"
     CDS             complement(8414..9385)
                     /locus_tag="ACHA6B_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8949651.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="ACHA6B_002095"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="ACHA6B_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8949652.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DAYYHB010000078        11430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000078 DAYYHB010000000
VERSION     DAYYHB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.079"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1130)
                     /locus_tag="ACHA6B_002096"
     CDS             complement(81..1130)
                     /locus_tag="ACHA6B_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8949653.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1212..1901)
                     /locus_tag="ACHA6B_002097"
     CDS             complement(1212..1901)
                     /locus_tag="ACHA6B_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8949654.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2007..2375)
                     /gene="rplL"
                     /locus_tag="ACHA6B_002098"
     CDS             complement(2007..2375)
                     /gene="rplL"
                     /locus_tag="ACHA6B_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8949655.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2428..2931)
                     /gene="rplJ"
                     /locus_tag="ACHA6B_002099"
     CDS             complement(2428..2931)
                     /gene="rplJ"
                     /locus_tag="ACHA6B_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8949656.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3135..3262)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3341..4030)
                     /gene="rplA"
                     /locus_tag="ACHA6B_002100"
     CDS             complement(3341..4030)
                     /gene="rplA"
                     /locus_tag="ACHA6B_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8949657.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4134..4556)
                     /gene="rplK"
                     /locus_tag="ACHA6B_002101"
     CDS             complement(4134..4556)
                     /gene="rplK"
                     /locus_tag="ACHA6B_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8949658.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4899..5567
                     /gene="sdaAB"
                     /locus_tag="ACHA6B_002102"
     CDS             4899..5567
                     /gene="sdaAB"
                     /locus_tag="ACHA6B_002102"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8949659.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5580..6452
                     /gene="sdaAA"
                     /locus_tag="ACHA6B_002103"
     CDS             5580..6452
                     /gene="sdaAA"
                     /locus_tag="ACHA6B_002103"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8949660.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6543..7643)
                     /locus_tag="ACHA6B_002104"
                     /pseudo
     CDS             complement(6543..7643)
                     /locus_tag="ACHA6B_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7735..8622)
                     /locus_tag="ACHA6B_002105"
     CDS             complement(7735..8622)
                     /locus_tag="ACHA6B_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8949661.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8699..9244)
                     /gene="nusG"
                     /locus_tag="ACHA6B_002106"
     CDS             complement(8699..9244)
                     /gene="nusG"
                     /locus_tag="ACHA6B_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8949662.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9345..9515)
                     /gene="secE"
                     /locus_tag="ACHA6B_002107"
     CDS             complement(9345..9515)
                     /gene="secE"
                     /locus_tag="ACHA6B_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8949663.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9539..9691)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_002108"
     CDS             complement(9539..9691)
                     /gene="rpmG"
                     /locus_tag="ACHA6B_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8949664.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9853..10497
                     /gene="cbpA"
                     /locus_tag="ACHA6B_002109"
     CDS             9853..10497
                     /gene="cbpA"
                     /locus_tag="ACHA6B_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8949665.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10553..11056)
                     /locus_tag="ACHA6B_002110"
     CDS             complement(10553..11056)
                     /locus_tag="ACHA6B_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8949666.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11069..11115)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3336 a         2372 c         2089 g         3633 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtaa atacagtatt atttcggttg
      121 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
      181 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
      241 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
      301 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
      361 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
      421 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
      481 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
      541 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
      601 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
      661 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
      721 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
      781 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
      841 gcaaaaaaag cggccaacag agagacggca gataacgcta ccattcgaat gatcaatttt
      901 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
      961 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
     1021 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
     1081 aatagtaaaa taagcgaagt tgttaaaggt gttttttcat tactcttcat tgatttcccc
     1141 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
     1201 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
     1261 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
     1321 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
     1381 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
     1441 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
     1501 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
     1561 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
     1621 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
     1681 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
     1741 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
     1801 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
     1861 cccaacgaga tcaacatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
     1921 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
     1981 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
     2041 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
     2101 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
     2161 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
     2221 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
     2281 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
     2341 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
     2401 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
     2461 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
     2521 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
     2581 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
     2641 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
     2701 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
     2761 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
     2821 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
     2881 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
     2941 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
     3001 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
     3061 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
     3121 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
     3181 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
     3241 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
     3301 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
     3361 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
     3421 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
     3481 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
     3541 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
     3601 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
     3661 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
     3721 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
     3781 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
     3841 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
     3901 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
     3961 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
     4021 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
     4081 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
     4141 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
     4201 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
     4261 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
     4321 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
     4381 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
     4441 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
     4501 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
     4561 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
     4621 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
     4681 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
     4741 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
     4801 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
     4861 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
     4921 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
     4981 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
     5041 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
     5101 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
     5161 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
     5221 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
     5281 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
     5341 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
     5401 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
     5461 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
     5521 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
     5581 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
     5641 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
     5701 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
     5761 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
     5821 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
     5881 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
     5941 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
     6001 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
     6061 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
     6121 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
     6181 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
     6241 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
     6301 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
     6361 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
     6421 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
     6481 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
     6541 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
     6601 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
     6661 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
     6721 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
     6781 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctaat
     6841 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
     6901 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
     6961 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
     7021 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
     7081 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
     7141 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
     7201 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
     7261 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
     7321 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
     7381 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
     7441 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
     7501 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
     7561 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
     7621 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
     7681 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
     7741 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
     7801 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
     7861 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
     7921 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
     7981 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
     8041 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
     8101 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
     8161 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
     8221 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
     8281 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
     8341 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
     8401 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
     8461 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
     8521 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
     8581 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
     8641 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
     8701 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
     8761 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
     8821 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
     8881 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
     8941 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
     9001 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
     9061 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
     9121 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
     9181 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
     9241 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
     9301 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
     9361 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
     9421 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
     9481 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
     9541 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
     9601 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
     9661 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
     9721 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
     9781 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
     9841 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
     9901 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
     9961 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    10021 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    10081 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    10141 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    10201 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    10261 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    10321 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    10381 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    10441 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    10501 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    10561 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    10621 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    10681 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    10741 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    10801 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    10861 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    10921 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    10981 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    11041 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    11101 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    11161 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    11221 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    11281 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    11341 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    11401 cgaaccttgg aacttctcct tacgtaagta
//
LOCUS       DAYYHB010000079        11254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000079 DAYYHB010000000
VERSION     DAYYHB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.081"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHA6B_002111"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHA6B_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8949667.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHA6B_002112"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHA6B_002112"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8949668.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHA6B_002113"
     CDS             complement(2169..2906)
                     /locus_tag="ACHA6B_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8949669.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3003..3524)
                     /locus_tag="ACHA6B_002114"
     CDS             complement(3003..3524)
                     /locus_tag="ACHA6B_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949670.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHA6B_002115"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHA6B_002115"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8949671.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHA6B_002116"
     CDS             complement(4401..4886)
                     /locus_tag="ACHA6B_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8949672.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHA6B_002117"
     CDS             complement(5039..5236)
                     /locus_tag="ACHA6B_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949673.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHA6B_002118"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHA6B_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8949674.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHA6B_002119"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHA6B_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8949675.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACHA6B_002120"
     CDS             9211..9423
                     /locus_tag="ACHA6B_002120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8949676.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACHA6B_002121"
     CDS             9425..11104
                     /locus_tag="ACHA6B_002121"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8949677.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3266 a         2429 c         1970 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagcaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc aacaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 cttcaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DAYYHB010000080        11214 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000080 DAYYHB010000000
VERSION     DAYYHB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.082"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACHA6B_002122"
     CDS             complement(41..1006)
                     /gene="nrdF"
                     /locus_tag="ACHA6B_002122"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8949678.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACHA6B_002123"
     CDS             complement(1056..3215)
                     /gene="nrdE"
                     /locus_tag="ACHA6B_002123"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8949679.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACHA6B_002124"
     CDS             complement(3374..3598)
                     /gene="nrdH"
                     /locus_tag="ACHA6B_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8949680.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3999..4472
                     /locus_tag="ACHA6B_002125"
     CDS             3999..4472
                     /locus_tag="ACHA6B_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8949681.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4469..6601
                     /gene="feoB"
                     /locus_tag="ACHA6B_002126"
     CDS             4469..6601
                     /gene="feoB"
                     /locus_tag="ACHA6B_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8949682.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6603..6737
                     /locus_tag="ACHA6B_002127"
     CDS             6603..6737
                     /locus_tag="ACHA6B_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8949683.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6831..7475
                     /locus_tag="ACHA6B_002128"
     CDS             6831..7475
                     /locus_tag="ACHA6B_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8949684.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7662..8855
                     /locus_tag="ACHA6B_002129"
     CDS             7662..8855
                     /locus_tag="ACHA6B_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8949685.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8848..10575
                     /locus_tag="ACHA6B_002130"
     CDS             8848..10575
                     /locus_tag="ACHA6B_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8949686.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            10969..11166
                     /locus_tag="ACHA6B_002131"
     CDS             10969..11166
                     /locus_tag="ACHA6B_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8949687.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         3528 a         1972 c         2332 g         3382 t
ORIGIN      
        1 ctttttattt ttcgaaaaat ttttgaatta aaataaatcc ttattccaat ccaatcaaat
       61 aatcgtcgtc tttcatcgct tctacatgac ctaacaaata gccgtttccg acttgtgaga
      121 agaagtcatg gttgcttgtg cctgttgaaa tcccgttcat tacgattggg ttgacatcat
      181 tggcagtatc tggaaagagt ggatccatcc ctaggttcat caatgctttg ttggcattat
      241 agcgtaggaa tgtcttgact tcttctgtcc agccgattgg atcgtataat tcttctgtat
      301 aacgttcttc gttttcgtaa agctcataaa gaagattgta catccagtct tttaacgttt
      361 cttgttctgc ttcaggtaac tcgttgaatc ctaattggaa tttgtaaccg atgtaggttc
      421 catgtacaga ttcatctcgg atgattagtt tgatgatctc tgctacgtta gccaatttgt
      481 tgtttcctaa ataataaaga ggagtataaa aacctgaata gaacaagaat gtttctaaaa
      541 agacgctggc aattttcttt tctagcggtg taccgtgttt gtagatttca ttgatacgct
      601 cagctttttt ttgcagataa ggattcgtat ttgtccagtc aaaaatttct tcgatttctt
      661 ttttcgtatt cagtgtacta aaaatagaag aataactttt ggcatgaacg gattccatga
      721 attgaatatt gttcaaaacg gcttcttcat ggggagtgcg gacatcttta cgcaactgat
      781 ccatgccact ttcagattgg acagtatcta gcagtgtcaa accgccaaat acatgtccta
      841 ctgtatcttt ttccaattga gacaatgtac gccaatcatc taggtcattt gaaagtggga
      901 tacgtgtatc tagccaaaat tgctctgtca gcttctccca agtggattta tcaatgatat
      961 cttcaatttc attccagttt attgcttcat aatatgtttc tgacatgaga atgctccttt
     1021 aattaaaata tgaataagcg aatctatcta tgatattaga tcacgcagct ttcacattga
     1081 ttgcttccga tttcttctgc atcatcagta aaggtacgta cgtaataaat ggatttgatt
     1141 cctttgtgga aagcgtaatg tcttaaaata ttcaaatcac gtgtcgtttg cttcgttgtt
     1201 gtcttccatt cataaatgcc ttctgggatt tcagaacgca tgaataaagt caaactcatg
     1261 ccttgatcga cgtgttgttg tgccgttgca taaacatcga ttactttacg catgtccata
     1321 tcataagcag aagtgtaata cggaatcgtc tcattggata gataaggcgc tgggtaataa
     1381 attttaccga ttttcttctc ttggcgttct tcgatcatgc gagtgatagg atgaatactt
     1441 gcactcgtat cgttgatata tgaaattgag ccatttggtg ctactgccaa gcgattttga
     1501 tgatacagtc catctttcat tactgcttgt ttcaaagcat tccaatcttc agcagttggg
     1561 ataaaaatgc cttcaaacaa tgctttgact ttttctgatt ttggctggaa ttctccagtt
     1621 acgtattttt cgaaataagt gccgtcagca taagctgatt tttcaaaatt atggaaaact
     1681 tgttttcttt cacgagcgat attgttgctt tcaacaagtg tccaataatt caacagcatg
     1741 aagtaaatat ttgtaaagtc taatgaatcc tctgagccat attccatttg ttcttttgca
     1801 agataagtgt gtaagcccat ggcacctaat ccaatcgtat gattcagctt atttccgttt
     1861 tggatcggtg gtacgacatc gatatcagat gcatctgtaa taaatgtcaa tgcacgagtc
     1921 atcgtacgca cagattgacc aaagttaggg ctttgcatca aattcacgat atttgtcgag
     1981 cctaagttac atgagatatc tgttcctaat acttcatatt cctgtttgcc gttaatcaca
     2041 gaaggcgttt gaacttgtaa aatctctgaa cataaattac tcatgatgat cttgccatcg
     2101 attggattct cgcggttcgc tgtatcaata ttgattacgt aaggatagcc agattcttgt
     2161 tgtaattttg agatttcatt ttcaagatca cgcgctttga ttttttgttt gcgaatgttt
     2221 ggatttgcaa ctagatgatc atactcagct gtaatattta cataagagaa tgggacacca
     2281 tattcttttt cgacactata tggactaaat aaatacatat cttcgttttt acgtgtcaat
     2341 tcataaaatt tatcaggtac gatcacgcca agagaaagag tttttacgcg aatcttttca
     2401 tctgcatttt ctttttttgc tgaaaggaaa gcaataatgt ctggatggaa aacgttcaaa
     2461 taaaccacgc cagctccttg acgctgacct aattgattag aatagctgaa actatcttcg
     2521 aataatttca tgactggaac gacaccactt gcagcaccct catatccttt gataggagcg
     2581 ccggcttcac gaagatttga taaagtaatc cctacgccgc caccaatacg ggacaattgc
     2641 aaagcagagt tgattgagcg cccgatagag ttcatatcgt cagtgacttg agtcaagaaa
     2701 caagacacca attcgccccg acgtttacgt ccagcattca agaaagaagg tgtagctggc
     2761 tgatatcgtt gatggatcat ttcgtctgca agagcaagtg ctaattcttc atccccgttc
     2821 gcaaagtaaa gcgcattgaa ggctactcga tcttcataag tttccagata agcggaaccg
     2881 tcgttggttt tcaacgcata ttgagaataa aatttgtaag cagccataaa agatttgaaa
     2941 gaaaaatgtt gctcgtccaa aaattgatat agcttttcaa taaatgatgg tgaatacaat
     3001 tgaatgaatt ctgcttctat atattcttga tcgatcaagt aattgatttt ttccataata
     3061 gatggaaaag tcatcgtatt tggtttgaca ttttcctcaa aaaaggcaac taaagcttct
     3121 ttatccttat tcaaagggat ttgaccattg actggccgat tgatttcatt attcagcttg
     3181 aaataactga catcttgtaa atttttaaga ctcatacgta cgcccaacct tcatttcgta
     3241 tttgatgatg cttgattgca aatcattgca ttgtttttaa gctttaaaaa aggatttgga
     3301 agtttgcgac aaaaactggt gctgtctcaa gcccttcaaa tccatgtatc acgttttgat
     3361 tcttttagtc atgttagctg gcaagtttac gcaactgatc aggacggaat ccgataactg
     3421 tttctgcatc agaagtaatg acaggaacac tttggaatcc ttgtgctttc aaccagtcta
     3481 ctgcttcagg ctgttcatcg atattgactt cttcaaaagc aatttgattt tctgttaaaa
     3541 accgtttgac catcttacat tgcatacaat tatttttaga aaagattttt acgttcatgg
     3601 tgagtgcctc ctctaacaca ttcttattat ctataataga tagtataaaa ctcttagtaa
     3661 aaaagtcaaa caacaaacac tacatattgt gttgtggaaa ctgtggaata ctaagttttg
     3721 tgtttttgaa aaaagagctg aaaacaagta tcgtgtggga aaatagcaaa tttaatagag
     3781 aaaaaattgt ggaaaactat gaagaaatat ttttttctca tttttatcta tcaatactaa
     3841 agattcgctc ataatatcac aagtataatt gacattctca attaccgaga gtattctatg
     3901 tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa ttagaacata taatgaatga
     3961 ttttaataca tgatttattt cacaagagag gaatcaatat gcagacctta gcgcaggcaa
     4021 agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga agaaaaaaca aaacgccatt
     4081 tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt cgtcaattta tcaggcaaca
     4141 atgccatttt actggtaaaa agtcatcgag tggcagtaag tcgggaaata ctaggtcaaa
     4201 tctttgtcaa taaaaagact gcttcctctg aaagctggac ttctcttgat caattaaaag
     4261 caggagagac tgggatcgtc gtagcaatcc acggacaagg cgcagtgaag cgacggttaa
     4321 tggatatggg attgacgcga aatgtaaaaa tagttatccg aaaactagcc cctctgggag
     4381 atccaatcga actgacagtg cgagggtatg aactgacgtt acgaaagtca gaggccgaac
     4441 taatcttgat acaaagggag gaaaaagaat gaatatcgcg ttagtcggaa atccaaacag
     4501 cgggaaaact agcttgttca atctcttgac tggttcgaat caatatgtag ggaattggcc
     4561 gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa aatccgaaca ttcatattca
     4621 ggacctaccc ggcatctatt ctttgtctcc ttatacacca gaagaagtga ttgcaagaga
     4681 ttatttggtt caggaacagc ctgaactgat catcaacatt attgatgcta ccaatttaga
     4741 acgaaatctt tatttaacta cgcagttatt ggatttagac attcctatga tcattggtct
     4801 gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc aatctagaaa agctttccta
     4861 tggtcttggt gtaccagttt taccaatcag cgtattgaaa aagcaaggaa tcgaaaaact
     4921 aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct attcctgttc cctgtacata
     4981 tgatccacgt ttagaagcag ctttacatga aattgaagat gtactaggag aacaaagcgg
     5041 caagaaacgc tggtatgcaa tgaaatattt tgaacgagat cagaaggtaa gagaggatta
     5101 cgagatagca gtttctcagc aaaaagagat cgagcaactg atccagttaa cagagaaact
     5161 attggacgac gatagtgaga cgattctggt caatgaacgt tatgaattta ttacccaact
     5221 atgtacactc tgtgttgtgt caaatgattc tttccaatta tcgatgagtg acaaaatcga
     5281 tcagattgca acgaatcgtt ggttagcctt gccgatcttc gcatttgtga tgtggctgat
     5341 ctattatctg gctattcaaa cagtaggaac aatggggaca gattggatca atgacacatt
     5401 gtttggtaca tggttaccag aacatgttgg cagattgtta gctgaatggc aagtagcggg
     5461 atggatgcag gagctgatct tgaacggcat catcgcaggc gtgggagcag tgttaggatt
     5521 tctaccgcaa ttgattatat tgtttttatg cttgagcttt ctggaagact gcggatatat
     5581 ggcgaggatc gcttttgtca tggatcgact tttcaggaaa ttcggtcttt caggaaaatc
     5641 cttcattcct atgctgattg ctacaggatg tggagtgcca ggcgtcatgg ccagtcgcac
     5701 gattgaaaac gagcaagatc gtcgtttgac gatcatggtc acaaccttca tgccttgttc
     5761 tgcaaagttg ccgattattg ccttagtagc aggggccttt tttcctcatc aatcttgggt
     5821 agctccttct gcctattttc ttggtatgac tgcaattatt ttctctggaa tcagcttgaa
     5881 aaaaacacgt ttgtttgctg gagatacagc tccttttatc atggaattgc ccgcatatca
     5941 ttttcctcaa tggtcagcgg tcctaagaca aacctatgac cgcagcaagt cttttgttaa
     6001 aaaagcagga acgattatct ttgtttctag tatcgtgatc tggttcagct cttcttataa
     6061 tttttatgct caacctgttt ctgaagacca aagtatctta gcctttttcg ggcgaatcct
     6121 ggcagttgtt tttcagccgc ttggttgggg aaactggcaa ggaacagtcg cagcgattac
     6181 aggattggtc gctaaagaaa atattattgg cacatttggt attttgtttg gtcatgcgga
     6241 agtatccgaa aatggcagag aaatctggca agtgctacaa cacacatata ctcccgcagc
     6301 agcttattca ttgttagcct tcaatttact ttgtgcgcct tgttttgcgg caattggggc
     6361 tatccatcgg gaaatgaatg ccaagaaatg gacttggatc gccataggct atcaatgcgg
     6421 tttagcttat ttagttagtt ttgtgattta tcagttggga cacttattgg agggtggaca
     6481 ggtagcgtta gggacgtatc ttgctatttt actgatgata ggactcgttt atgttttgtt
     6541 ccgtcagcca aaatccaaga accagtttta tactatattg tcattagaag gagaggaata
     6601 ggatggcgac attcttatta agtgtgttaa tctttggaag tgcaggcgca attgtctata
     6661 ctcgtttaaa atccggtaag aattgtgatg actgtcaaac agcttgccca gtaaaaaagg
     6721 aacagatcaa gcgatgatca gtcgttgcaa gaggctattc tccccttttt tgcttatttt
     6781 gctagagcat agtaagatga gggaaagtaa aaaaagaaag aggaaagaaa atgtctggaa
     6841 agaaaaaaat cgtccgcccg cgctaccagc agattgctgt tgatttagcc gaacgtatcg
     6901 ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg ctcaacactt gccagtacgt
     6961 acaatgcttc tcctgagact acccgcaagg ctatcaatgt gctggtagat cttggaatca
     7021 tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa ggaaaaagca aaagatttcc
     7081 tattgcaata tcaagatgta caatccatcc aagatacaaa agcagaaatc atggcaagtg
     7141 tggagaagca acaagatgag ctgaatcatt tttctcaatt attggatcag ctggtcaatc
     7201 aaacgaagcg aatacataag atgaatcctt tacttccgtt tgaattatct ttatcagaaa
     7261 aagcaatgca tctggaccgt tcaattagcg agttgaatat ctggcaggcg actggcgcca
     7321 cagttatcgc aatttctcat aatgaagaac tgcttgtttc acctggcccc tatgctaaat
     7381 ttacggcagg ggacatcgtt cattttattg gaaatgaata tacactacag cgtatgacaa
     7441 acttttttta cccggagcat cagcttgcag aatgattgtg tgaaatatta aaaaaatatt
     7501 aacttggcta aaccttttat ttacaaagat ttagccaagt ttttttcagt ttggacaagc
     7561 aaatttcttt catttttgac aaagtgacta catgatgtta acatagtgtg gtcacttttt
     7621 tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa gtagaagtaa
     7681 aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg atgaatgatc
     7741 accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg tatgacgtga
     7801 attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg agtgggaaat
     7861 ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt atctatattg
     7921 atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt cgtcataaaa
     7981 tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg gaaaatacag
     8041 aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa gcagaaaaag
     8101 cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag ttatctggtg
     8161 ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag atcttgctga
     8221 tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa gacgaattac
     8281 tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat ttgaatgaag
     8341 ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg cagatcggaa
     8401 caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt gtggaagaag
     8461 tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg acgacgaata
     8521 tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa gtcagcatgc
     8581 tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat caagcgttag
     8641 aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg acggtgattg
     8701 ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg actccacttg
     8761 cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg attgaagcat
     8821 tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca attaccagtg
     8881 gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg cctattttca
     8941 tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact gttagtgatt
     9001 ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc taagaaacgt
     9061 ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca aggtctatgg
     9121 aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc gatcatcatt
     9181 ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat tatcaaaccg
     9241 atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc agttgcattt
     9301 ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct gccaccaacc
     9361 gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga agcttctgat
     9421 tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt agctaaaagt
     9481 acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt cgttattgcc
     9541 tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca aagagcacaa
     9601 gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat cattgaccgt
     9661 tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac aacacagaaa
     9721 agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt aggtgcgatt
     9781 ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc ttatgttgaa
     9841 tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca aatgggctat
     9901 gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc aaatggcgag
     9961 tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata tgcacaatat
    10021 aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg tttagcagtg
    10081 ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg taagaaaatc
    10141 ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat ccaaaaatat
    10201 gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac cgttgcacta
    10261 ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc tcattggatg
    10321 tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg aagcgaagag
    10381 cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc ttacaaagta
    10441 ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga aatcaacaat
    10501 ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga attagtcgaa
    10561 agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat tggatcaaat
    10621 atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa aaagagaatg
    10681 cgtagctcat cttcgaaacg ttcaaagacg ccgacaaact attatgaatt tatgggtgcg
    10741 agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta ttatgtacga
    10801 agtgcattct caaaaaatat taaagaatat acatagttta tatacttaaa agtgattaaa
    10861 ttcttattca ggaattattt tgctacgttt caagaaggaa ttgttttttg aaatttaatg
    10921 aatgttagaa taatagtcta aaaacattaa tttaggggtg attagattat gaaacattta
    10981 aaaattttgt ctattaaaga gacacaactt atctatgggg gtaccactca tagtggaaaa
    11041 tattatggaa atggagtgta ttgcactaaa aataaatgta cggtcgattg ggccaaggca
    11101 actacttgta ttgcaggaat gtctataggt ggttttttag gtggagcaat tccagggaag
    11161 tgctaaaatg aaaaaaaatg ctaagcaaat tgttcatgaa ttatataatg atat
//
LOCUS       DAYYHB010000081        11150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000081 DAYYHB010000000
VERSION     DAYYHB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.083"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA6B_002132"
     CDS             6..305
                     /locus_tag="ACHA6B_002132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949688.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA6B_002133"
     CDS             378..1739
                     /locus_tag="ACHA6B_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8949689.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA6B_002134"
     CDS             1761..2021
                     /locus_tag="ACHA6B_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949690.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA6B_002135"
     CDS             2035..2787
                     /locus_tag="ACHA6B_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8949691.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA6B_002136"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA6B_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949692.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACHA6B_002137"
     CDS             complement(3542..4516)
                     /locus_tag="ACHA6B_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8949693.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACHA6B_002138"
     CDS             complement(4894..5613)
                     /locus_tag="ACHA6B_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8949694.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACHA6B_002139"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACHA6B_002139"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8949695.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACHA6B_002140"
     CDS             7164..8561
                     /locus_tag="ACHA6B_002140"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8949696.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACHA6B_002141"
     CDS             8642..9082
                     /locus_tag="ACHA6B_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8949697.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="ACHA6B_002142"
     CDS             9229..9555
                     /locus_tag="ACHA6B_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8949698.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="ACHA6B_002143"
     CDS             9556..10305
                     /locus_tag="ACHA6B_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8949699.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="ACHA6B_002144"
     CDS             complement(10331..10741)
                     /locus_tag="ACHA6B_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8949700.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DAYYHB010000082        11150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000082 DAYYHB010000000
VERSION     DAYYHB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.084"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1379
                     /locus_tag="ACHA6B_002145"
     CDS             3..1379
                     /locus_tag="ACHA6B_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949701.1"
                     /translation="MLISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEA
                     IALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLF
                     LSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSI
                     SDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFP
                     WFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVT
                     SIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVK
                     VGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLV
                     NVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKE
                     INKKNENK"
     gene            1366..3000
                     /locus_tag="ACHA6B_002146"
     CDS             1366..3000
                     /locus_tag="ACHA6B_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8949702.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3175..4008
                     /gene="msrA"
                     /locus_tag="ACHA6B_002147"
     CDS             3175..4008
                     /gene="msrA"
                     /locus_tag="ACHA6B_002147"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8949703.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4088..5077
                     /locus_tag="ACHA6B_002148"
     CDS             4088..5077
                     /locus_tag="ACHA6B_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8949704.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5366..6064
                     /locus_tag="ACHA6B_002149"
     CDS             5366..6064
                     /locus_tag="ACHA6B_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8949705.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6214..7530
                     /locus_tag="ACHA6B_002150"
     CDS             6214..7530
                     /locus_tag="ACHA6B_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8949706.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7547..8962
                     /locus_tag="ACHA6B_002151"
     CDS             7547..8962
                     /locus_tag="ACHA6B_002151"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8949707.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9426..9746
                     /locus_tag="ACHA6B_002152"
     CDS             9426..9746
                     /locus_tag="ACHA6B_002152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949708.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            9772..10089
                     /locus_tag="ACHA6B_002153"
     CDS             9772..10089
                     /locus_tag="ACHA6B_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8949709.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10118..>11150
                     /locus_tag="ACHA6B_002154"
     CDS             10118..>11150
                     /locus_tag="ACHA6B_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8949710.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         3708 a         1767 c         2235 g         3440 t
ORIGIN      
        1 cgattctgat tagtttttat tatggagaaa agttttttaa aaagatagat gctgcttttt
       61 tatttagttg tttatatact tttgcaactt atcgaacagt tgatatctat tatcgatccg
      121 caattgcaga agcaatcgct cttacgatta tcccagctgt tttatattat gcttatggat
      181 tgatattcaa tagagaaaaa aaagtcatcg ctttatctgt cagtataagt ctacttgtgt
      241 atactcatgt cttaactaca ctattgtgta gtgcattgat tgcttgtatg attatagttc
      301 gtttgttttt aagcaagcaa ataaaaaacg atataaaatt tgtggtcgtg aatttagtaa
      361 aggctgtttt tttgacttgt gcattaacta gctatttttg gtatcctatg ttgcagcaga
      421 tgaattatca agaaatacac agtcccttca aaacagattt gcagctagaa gcactttcta
      481 tttcagatag tatcattcaa gcgttaaata acgatattac aacatacact atggggttat
      541 taggattggt tgcgttgatt tttcctttat tcatactgaa agaaatggag aaaaaagaaa
      601 aaatcatcta tgttggcgta gtttctacat gggtatgcgt aactaattta tttccatggt
      661 ttttgtttca aaaaacaccg ataagcttac tccagtttcc ttggcgtatc ttaggtatcc
      721 aaatcttttt ttcttcaatg atcataacgc ttgtttttat gaaaaagact atgaataaaa
      781 aatattcatg gttaatcatt ggcgctactg tggtgtttct actcgtcacg tctatagcag
      841 caaaagaaaa ttacgcaaaa gttatccaat caaaacacgg tcatattgtt atcaatgaag
      901 aaacactccc acgttataca acatcagata tgggaggatt atacgattac gccccaacag
      961 aagcgataaa agaaaggcct cacttagaaa aacatgaagt aaaagtagga gaatcgtggg
     1021 aaaaagggaa gtataaagct gcagataata acattactta cacagtggca agcaacaaaa
     1081 agcaaaaagt gacagtacca gtatatgctt atttaggtac acaggtaaaa gtaaacggta
     1141 aagtcgtaaa tgactcttat aaaaaacatg gcttagtgaa tgtggatgta aatgctggag
     1201 aaaataagat agaaattaca acaaaatata ctccaactgc tctcatggct ttatggatca
     1261 gtattctgac ttattgttta gtggtgtatt caagttttaa acgatttgca cctaataagt
     1321 tgaaagattc tcgatatgtg actaaaaagg agataaacaa gaagaatgaa aataaataaa
     1381 aaagtagtaa tattgagtgg attaattata gttagtagta tatattcttt catctttttc
     1441 aaaaatacac agagtattta taccactgga gatctttctt ttcacttatc tcgaatcaag
     1501 gggttatcga ctatttttac cagtccgatt aattatgaga cattcaatta tactggatat
     1561 ggtgtaaatt atttttatcc ctttctgact ttttttccag ctgtaatgtt atattggatg
     1621 acaaaaaatt tgattgtaag ctatattatt tacgtttggt tattgaactt gtgtacaaca
     1681 ctcgtagctt atcattatgg cgagcggttt ttaaaacaaa aaaaagcggc atttttattt
     1741 agttgtctct atattttctc tgcttatcgg acagtagaca tctactatcg ttccgcaatt
     1801 gccgaagcta tcgcaataac acttgttatc cctgtattat tttatgctta tcaaatcatc
     1861 tctggaaaag aagaaaagta tccgtctgta aagctagcat tgagtatgag tttgctagtg
     1921 tacagccatg tactatcaac attaatgagt acagcattaa ttatcatttt tatttttatt
     1981 cgtttggttt ctaaaggttt caagaatgca gattttattg ctatatttaa aaaattattt
     2041 agtgcggcag gaatgacatt ggttctgacg agtgcttttt ggtatccgat gtttgaacaa
     2101 atgctctacc aaaaaatcaa caaacctagc gtgacaaatt tatatgctca tgcatctaat
     2161 gtgttcgata gtctgactga agctatgaac aacgacttaa ctacttactc aatgggacta
     2221 gttggtctgt tatctttatg tattcccctg attttattta aaaaattaac aaacatagaa
     2281 aaaaagattt attatggtac gtgtttgact tggctagcta caactagttt ggtgccatgg
     2341 tatcttctgc aaaatacgcc agccaaattg ttacaatttc cttggcggat cttaagcttg
     2401 caaataatat tctcttctct gatattaacg atgatatttt tcaaaaatag aagatacaat
     2461 aagacaagag aactatttta tttatttatg actgttttgt tattaatcac tttaactgtt
     2521 tctagtaaaa aaaatttcaa tcaaaaaata atttctcagc cgggacatat tgttgtaaac
     2581 aaagaaacag tagaggcgta taccactaaa ggaatggatt attttttata tgattattct
     2641 cctatcgctt cattggagaa taaggagcat ataataaaac acgaagcaaa gattggcagt
     2701 caatgggtaa accttcctta taaaacaact ggtagttcta tcatatatag agtaactaat
     2761 gagaagagcc aagtaatcac acttcctgta tatgcttatt taggaacgaa tgtagaaata
     2821 aatcatcaac ctgtaaagga gcaggtgaat gctaatggat tggtacaaat aagcggagtc
     2881 aaaggagttg ctgatattaa agtaagcaca agttatacag taacgactat tatttcttat
     2941 cttgttacat taactgcata tttatgttta ttttttaaaa tagttttcca taaaaggtaa
     3001 attaactttt catttataaa ataaattgat taaaaaggag aatctgtcga taaggattct
     3061 ctctttttta tacgaaaaat ctgtaggggg atgaagtaat cgcttatttc ctcactttat
     3121 gataaattac agctaaagtc actagcttgt cttcttcgga aaggaggaga atctatggat
     3181 gaggaacaaa tattcattca aacactttat aatctgattt tgaatccgaa tacacgagat
     3241 tgggaacgaa aagtattgat tcagacaaag aatgacataa gagaaaatat atccgttaaa
     3301 gaacagttat caaaactaga agcaacacta cgtccattag ctatccgaat gaatctgacg
     3361 cctgatgtaa tggactttta tttattgatt acggaaggat ttgataagga gcaaaaatat
     3421 gacttttcca agcatgccat gcaagatgct gactatcaag aacgtgcagt atttgcaggg
     3481 ggatgtttct ggtgtatggt cgagccgttt gaatcgaaaa aagggatcct atctgttttg
     3541 tcaggataca cgggcggaca tgttgaaaaa ccaaattatg atcaagtaag tggtggttac
     3601 acaggtcatg tggaagctgt ggagatcatt tatgacacaa gagaaataag ttatagcgag
     3661 ttgctgacaa tctattggca aattactgat cccacagata catttggaca atttcaagat
     3721 agagggaagc aatatcgtcc agtgattttt tatcaagtcg aacgacaaaa agaattagcg
     3781 gaacaatcaa aacagaaact agacagttca ggaactttcc accaaccaat cgtcacaaaa
     3841 atcgaaccag ccggtacgtt ttggccagca gaaaactatc atcagcaatt ttacaaaaaa
     3901 caaccgaaac gttataaaaa aatccagcaa gcacgtaatc aatttctgat ctatcaacga
     3961 gtaaaaaaca aatggcaaaa aaatatacga aaaaatcatt ttgattaact ccttttcttt
     4021 aaaatgtagt aaactaaaaa tagaaaaaag aaagcgtttt aaaaagattt gatgaggagg
     4081 agaaaaattg tatattgcag atagtgcacg ttatgaaaaa atggaatatc gtaaatccgg
     4141 tcattcaggt ttgaaattac cgattttatc tctcggatta tggcaaaatt ttggtgatta
     4201 tgatcctatc cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca
     4261 tttcgacctt gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg
     4321 tatttttaga gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg
     4381 ttatcatatg tgggaagggc catatgggga atggggctct cgtaaatcca tcatttcaag
     4441 ttgtgaccaa agtctccaaa gaatggggct agattatgtt gacatcttct atcatcatcg
     4501 cccagaccct gatacacctt tggaagaaac agcagaagct ttgatgcaac ttgtaagaca
     4561 aggaaaagca ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaaatgac
     4621 tgaaatttta aaaagaaagg aagcaccgtt cattattcat cagatgcgct acaatatgtt
     4681 ttctcgtgca ctactagaag acgatctatc tccagtatta gaagaagaag ggctaggggc
     4741 aatcacgttc agtccgcttg ctcaagggtt gctgacaaat cgttatctac atggtattcc
     4801 tgaagattct agagcacatc gtaaggaaat accattcctt tcagaagaac aagttggatc
     4861 aactctggaa aaaatcaaag cactgcaaac aatcgccgta tcacgaggac aatcattagc
     4921 gcaaatggcc cttgcatgga atttgcgaca aaaatctgtt acaagtgtac tggtcggagc
     4981 aagtcggtta agtcagttac aagaaagtgt tcgaatgatg gataatttag atttctcacc
     5041 agaagaagaa ttgagaatcg accaaattct agaatagaaa cgatgtaatc aaacttttta
     5101 tccatatatt aaaaaaaaca aaagatgaca tcaagaatcg aatgaagatc gtctgttatt
     5161 gaacatcata ttttttgtcg ttcaaaataa aaatgatcga aggctaggag tgaattttat
     5221 cctagtcttt tttatggtaa atatcttctt cgtttgtatg tctatgaacg gattaatcaa
     5281 ttttgtatac aaaaaatcat cagatgattc aaacgtgtta taataattac gaaaaaatac
     5341 atcagatgaa aaggaggtat gtggtttgga agaaaataaa accctagtag aagtaactac
     5401 tgacaagatc cttagatatt taaaggaaat gcagttgcaa gtaggagata aactgccgaa
     5461 tgaatatgaa ttgtccgatt atttagaggt ggggcgaagc acggtgcgag aagctgtgcg
     5521 ggctctagct tccaggaata tccttgaagt tcggcaagga gcggggacat ttgtcagtga
     5581 taagcgaggg attgccaatg acccactcgg tttttctttg attgaagatc aggatcggat
     5641 gatacgagac ttatttgaac tgagatattt attggagcct aagatggcag gtttggcagc
     5701 ggaatatgca acgaagaatc aaatcgtcca aatgaaaaaa ttaacgcaaa agattgaaac
     5761 ttcttttcga gaaggcacac aagaacatgt tcaattagac atcgattttc atacgatgat
     5821 tgcagaagct agcggaaacg tagccttgct gcatattatc ccaatcatta atgaatcaat
     5881 tgctttgttt aataaaaatt atgatatcca gcatctgaaa atcgagacga tcgatattca
     5941 tacagagatc gttcaggcca tcgaaaggcg gaatgcactt gctgcgtttg atgcaatgac
     6001 tgtccatatg gcaataaatc ggagagaatt acagaaaata ataggagaaa aagaaactaa
     6061 ttgacagttc atctgatgta ttttatgata aatacatcag atgaattttt gttttatatt
     6121 tggtaaaaaa gaggagtaaa tagtttctgt tcatcgaaat gtaagcgctg actaatagac
     6181 aaaaagagat gacgtcaagg agaagaagca ggaatgaggg gatctggaat gagttttatg
     6241 gataaattag ccaatgtttt agggaacgtg gcaacgagaa tcaataattt aaggtatatc
     6301 atggtgataa aaaacgcatt tgctgcattg attccggtaa ttattacggg tgcttttggc
     6361 acattgtttt cagcaatggt ctttgactcg gaaaatggct tagcacagat aagtttcttg
     6421 cgttttttag aagaattaaa gcctatcgca tctgccatca gctatgtcac actaagtttc
     6481 ttgaccatct atgctgtttt tctgataggg atcgaattag ccaagctgaa taaagtggat
     6541 ggtgtattcc ctggtatcat cgcagtgatg tcttatttat ctgttaatcc aactgtttat
     6601 gaattagtga atgaaaatca gactgttata gtagaaaacg tattggctaa acaatataca
     6661 gatacgaaag ggttattttt gggaatgttg gtctccatcc tttctattga actttattgc
     6721 tggttgagga aacagaagaa actacagctg agaatgcctg ataccgtgcc aacgaatgtt
     6781 tctgctagtt tttctgcttt attcccgacg attctgactg tatcgggtat cgcggctgcg
     6841 ggatttatca tcaaagaact tacaggaatg tacctttatg atatcattta caatgtcgta
     6901 caacgcccat tagaaggtgt cgtacaaggg ttaccgggta ttttgctatt gatgtttatt
     6961 gcgcaagtct tttgggtgat tggtatccat ggaaatcaaa tggtgaagcc gatacgggaa
     7021 ccgttgcttt tagcggcaat tgctgtaaat acggaagctt ttgaagctgg aaaagaagta
     7081 ccgaacatta ttactatgcc attttgggat atgtatatga gtatgggcgg gtctggagtc
     7141 acgatcggtt tactgattgc agtattgtta gttggaaaac gggaagatat gaaacaaatc
     7201 accaagttat cccttgctcc tgggatcttc aacatcaatg aaccagttat ttttggtatg
     7261 ccgatcatgc tgaatccaat tttagctatt ccgtttatct taacgcctct tatcactgga
     7321 acgattggtt attttgcaac cgctttagga tttgcggcga aagcagttgt aatggtccct
     7381 tggccaatgc cgccgatcgt caatgcttat ctggcaacag caggaaatat cggtgcagtt
     7441 attacgcaac ttgtttgtat tattgtttct attttgatct atctgccatt tgtaaaagta
     7501 gcaaatcgtg tggaaaatga tgggatctaa aaaacaggag gaaatcatga aaattactat
     7561 tccaaaaaat tttattcttg gtgctgcatc ttctgcttgg caaacggaag gttggaaagg
     7621 aaaaaaagaa ggacaggatt cgtttataga tagctggtat aaaaatgaac attttgtttg
     7681 gcataatggg tatggcccag ccgtagcaac taattttatg gaacaatacc agcaggatgt
     7741 ggatctgatg aaatccatcc atctgacaca ttatcggact tctatcaatt gggctcgttt
     7801 ttttactgat tatgagaact gtatagtaga tgaagaatat gctcagcata taagtgaaat
     7861 gatcgactct ttgatcgaag cagacgtaga accgatgatt tgtttggaac attatgaagt
     7921 cccagctttt cttttggaaa aatatgatgg ctggtcttca agaaaagtag ttgattttta
     7981 tgctaagtat gctcaaattg cctttgatcg ttacggtgat agggtaaaaa actggtttac
     8041 attcaacgaa ccaatcgttc cccaaacgcg ttgctatttg gatgctgttc gctggccgca
     8101 tgaacaaaac acaaaaaagt ggatgatgtg gaattatcat aaagttttag caacggctaa
     8161 agcagtagag atttaccgca gcggaaatta tggtggaagg attggctgta ttttaaatcc
     8221 tgaaatggtc tatgctcgat ccaattctcc agaagaccaa aaagctgctt atatgtatga
     8281 tttgttttat aatcgagtat tttttgatcc gatggtaaaa ggatactaca cagaagagtt
     8341 cctggagttg tgtcaaaaac aatcgattta tttcgatcct gcaacagagg atttagaaac
     8401 gattcagcat catacagtag atttccttgg aatcaatcaa tattatccta aacgcgttcg
     8461 cgcccctcgt tatcaatgga atgaagagac acctttccat cctgaacgat attatgaagt
     8521 ctttgattta cctgggaaga aaatgaataa ttccagggga tgggaaatct atccgaaaat
     8581 catgtatgat atagccatgt atctaaaaga aaattatcat aatatccctt ggctgataac
     8641 agaaaacggt atgggaagag aatatgaaga acagtatatg gatgagaaag gaatcgtgca
     8701 agatgattac cgtatcgaat ttatcggaca gcatttgcat tacttgatca aagcaacaga
     8761 agaaggcgca aattgcgaag gctatatgtt atgggcattc actgactgtg tgtcaccaat
     8821 gaatgcattt aagaatcgat atggattagt tcgaatcgaa ttgaacgatg aacgaagcag
     8881 aacattgaaa aaatctgctt cgtggtatca gactttgatt gataagagag aattggattt
     8941 gcctgacgaa atctataaat aaatagaagt tttttgtaga tgttcattgt atttactggt
     9001 tatttgaaat aaaagaggtt gtgaatccag cgttttgagc taatacccag ggtcagctga
     9061 ttgaacaccg tttattgaga ggttgtgaaa aagctgtcct gcatcaagta ataagaatag
     9121 caaagtaaaa acaacatctt atgtttttaa cttggttgga cttattaccg cagatgcaag
     9181 cttttgaata ccgtttattt gaaaaaagtt gtgacaagtt ttgtgtcaca actttttttg
     9241 tgcacgttaa aagaaaaaag gtaataaaaa ttaatttgtg tgtttagtgt gcgctttctt
     9301 ttttagtgtg tatgcaactt ggtatagacc ttaaatgtga tgatatcaac tttttaaagc
     9361 gcttttaaag ttggcacgct acttgcttat atacaagtgt aagtatagaa aggaacgtgt
     9421 ttaaaatgga aaagaaaaca atcatgcttg tatgttcagc tggaatgagt acaagtctcc
     9481 tagtcacaaa aatgcaaaag gcagcagaag caaaaggaat ggaagcagac atctttgctg
     9541 tatctgcttc agatgcggat aatcaactag aatctaaaaa tgtagacgtt ttacttttag
     9601 gaccgcaagt acgctttatg caaaatcaat ttgctgaaaa gctagcacct aaaggcattc
     9661 ctttagatgt aatcaatatg gctgactatg gcatgatgaa tggtgaaaaa gtactcgacc
     9721 aagcagaaaa actaataaac aaataataaa aattatcgga ggatacaata aatggaagat
     9781 caaaaaaatc tagaagccat catgggtctg atcatgtttg gcggaaatgc aaaaagcgat
     9841 gcaatggaag cgattgctgc tgcgaaaaaa ggtgatttca ctttagcaga tgaaaaaatc
     9901 aaagatgcag aggaatcttt agtccaagca catcactctc aaacagagat gctgacacaa
     9961 gaagcacaag gaaatcacgt tgaagtcacg cttttgacgg tccacagtca agatcatctg
    10021 atgacatcta tagcttttac agatttagca aaagagatca ttgatctgta tcgtcgattg
    10081 aacgcgtaat ctacagtaaa gaaaagaggg aagatttatg gataaatttg ttgcatttat
    10141 ggagaaacat tttatacctg tagcatcaaa aattggtgca caacggcatt tagttgcgat
    10201 tcgggactca tttatggtga gtatgccgtt aatgatctta ggagcattag cagtaatgat
    10261 caataactta ccgattcccg ggttccaaga attgatgaac tcaatttttg gtggtgaatc
    10321 atggaaagga ttcggggctg ctgcttggaa cggtacattt gccattcttt ctgtcttgat
    10381 tgctttcttg cttgcttatc atttagctaa cggctaccga aaagacggtg tttccgcagg
    10441 tgtgatttct ttaggctctt tcttcgcttt aggcggagca ttaggtatga gctcaaacgg
    10501 tttgtttatt gctattattg ttgggattat ttcaacagaa atctttgtac gtttaagcgg
    10561 aaatgaaaaa ttgatcatca aaatgcctga tggtgtgcca ccggcagttg caaaagcatt
    10621 tgcttcatta ctaccagcaa tgatcactat ctctgcgttt gctttagttg ctgcaatttt
    10681 tgctgcattc ggtgttgatg atatcgtagg ttctttctat acagtggtcc aagaaccatt
    10741 tatgggactt gcaaactctt atccatctgc cttactgctt gcatttatca caccattctt
    10801 atggttcttt ggtttacacg gagcgaatat ggtcgatcct ttgatgcaaa caatcaatgc
    10861 gccagccatt gaagcaaacg ttaatgcaat cgcagctgga catagcgcac cgtttatcgt
    10921 gaacaaacct ttctttgaca gctttgtcaa cttaggagga acaggtgcaa ctttaggttt
    10981 actgttagct atttatttag taggacgtaa aaacaaacca tatatggttg taacaaactt
    11041 gtcgattgcg ccaggtgtct tcaatattaa tgaaccaacg atgtttgggt tacctatcgt
    11101 gttgaacccg atcatgttca ttccatttat tttgacacca atggtcttag
//
LOCUS       DAYYHB010000083        10885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000083 DAYYHB010000000
VERSION     DAYYHB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.085"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..1698
                     /locus_tag="ACHA6B_002155"
     CDS             229..1698
                     /locus_tag="ACHA6B_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8949711.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1709..3818
                     /gene="topB"
                     /locus_tag="ACHA6B_002156"
                     /pseudo
     CDS             1709..3818
                     /gene="topB"
                     /locus_tag="ACHA6B_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3834..3983
                     /locus_tag="ACHA6B_002157"
     CDS             3834..3983
                     /locus_tag="ACHA6B_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949712.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4064..4660
                     /locus_tag="ACHA6B_002158"
     CDS             4064..4660
                     /locus_tag="ACHA6B_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8949713.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            4673..5572
                     /locus_tag="ACHA6B_002159"
     CDS             4673..5572
                     /locus_tag="ACHA6B_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8949714.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            5575..6060
                     /gene="ssb"
                     /locus_tag="ACHA6B_002160"
                     /pseudo
     CDS             5575..6060
                     /gene="ssb"
                     /locus_tag="ACHA6B_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6082..6339
                     /locus_tag="ACHA6B_002161"
     CDS             6082..6339
                     /locus_tag="ACHA6B_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949715.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6498..6620)
                     /locus_tag="ACHA6B_002162"
     CDS             complement(6498..6620)
                     /locus_tag="ACHA6B_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8949716.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6775..7034
                     /locus_tag="ACHA6B_002163"
                     /pseudo
     CDS             6775..7034
                     /locus_tag="ACHA6B_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7482..7688
                     /locus_tag="ACHA6B_002164"
     CDS             7482..7688
                     /locus_tag="ACHA6B_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8949717.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7688..7939
                     /locus_tag="ACHA6B_002165"
     CDS             7688..7939
                     /locus_tag="ACHA6B_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949718.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            7955..8392
                     /locus_tag="ACHA6B_002166"
     CDS             7955..8392
                     /locus_tag="ACHA6B_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949719.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            8385..9080
                     /locus_tag="ACHA6B_002167"
     CDS             8385..9080
                     /locus_tag="ACHA6B_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949720.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            9245..9445
                     /locus_tag="ACHA6B_002168"
     CDS             9245..9445
                     /locus_tag="ACHA6B_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949721.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            9461..9640
                     /locus_tag="ACHA6B_002169"
                     /pseudo
     CDS             9461..9640
                     /locus_tag="ACHA6B_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9702..9886
                     /locus_tag="ACHA6B_002170"
                     /pseudo
     CDS             9702..9886
                     /locus_tag="ACHA6B_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            9920..10351
                     /locus_tag="ACHA6B_002171"
     CDS             9920..10351
                     /locus_tag="ACHA6B_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8949722.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            complement(10525..>10885)
                     /locus_tag="ACHA6B_002172"
     CDS             complement(10525..>10885)
                     /locus_tag="ACHA6B_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8949723.1"
                     /translation="VDIENSINEWLQSVPKNLFKSITFDCGKEFSSWKSISNTNDIDI
                     YFSDPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQ
                     TPLEVFLSYVNGKFCLA"
BASE COUNT         3905 a         1837 c         2266 g         2877 t
ORIGIN      
        1 attttaaaag gaaaaagtta tggaaaagag atccaaacgg cgttaccttc agaagaattt
       61 gcgctaggaa attcattccg attttcactt gagacagtgt cagaagtggc aaaacgaaaa
      121 aatgaaatct attatgaaca acaattgtca caaaaagaac gtgtataaaa caagtaaaaa
      181 agactatctg aaaaacggat agtctttttt cttttgggag gttttaaaat gaccgagaaa
      241 aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat
      301 ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt
      361 tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt
      421 gaattagcta aacttttttt catagacaaa gagatccaag accctaagca acaatttaaa
      481 gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga aaatcttcat
      541 tttgagacag aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga
      601 aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa
      661 gaagggttga ttgctgaact aaagccgaaa catgaacggc aaaatttttt agttggagac
      721 gaccgtttag atcatgcggt tgctttttta tggaaagatc cacagacagg agagacagta
      781 ggggcaagtt atcaaggaac gatcgtcgat tttaatcgtt tcggtaaacg gggaacgtat
      841 aaacacattg acaagaatcc tacgccaaat cacggattta atttaaaaat aggcgatccg
      901 aagcatttaa aattctttga aagtagtatt gatctgttaa gttacgctgc attgaatcgg
      961 gagaaactac aagatgcctg gttagtttcg atggacgggt taaaacatca tgtaatcagt
     1021 cattacgttg aagagtcgat ttctgaatta agacgaaaac aaacatttcc tcaatcgatt
     1081 gaaatatgtg tggacaatga tcgtgcagga catatttttt atgaaaaaga acagatgaag
     1141 ggaatcgttg atccttttac caataagaaa atccgttgtg agcgaggaat tccaaatgat
     1201 tggcaagtac ctaaagaata caaagctact tatgaagcag tcgcaaaaga aatgagtgtt
     1261 gaacctgaag caattatggc catacacaaa actgaaacta atttacagct gacaaatcaa
     1321 ttggtgtctg ctcatgatgt tcaatctacg tttggaaaga tgttggcgaa gggcgaacca
     1381 gtagaaacaa ttgatttgaa agaagcatgt accacggtcg cgaaggaact gaaagtatgt
     1441 gaacgcgcag atggaacata caactttgat cgtttttata gtagaaaagc caacataaaa
     1501 gacgttaatg caggaatcct tctttcctat aaagcagaac aatactacaa aggctataaa
     1561 aagcatgaac atgaatttgt acctgaagtg aaaaaggatt ggaatgatca attgaagcat
     1621 gagatccagc aacaagaaat cagaaagcaa aaacgtgcga tgttgtttca acaaggcaga
     1681 caacaagaaa gggagtgaag tcgtcactat gaaaaccgtc attttagcag aaaaaccgtc
     1741 acaagcgaag gcttatgccg atagcttttc taaagctacc cgaaaagatg ggtattttga
     1801 gatccaagat cgattgttta caggagaaac tgtgatcacg tatggttttg gtcatttagt
     1861 cgagttatat tcgcccgata tgtacgatga aaaatggaaa caatgggcat tagaacattt
     1921 gcctattttc cccaatcaat accactatca tgttcctaaa gataagaaaa agcagtttag
     1981 tgtcgtcaaa cgacagctgc agtcagctga tacgattgtg atcgcgactg atagcgatcg
     2041 tgaaggggag ctgattgcct ggtcaatcat tcaacaagca ggcgccaatc aaggaaaaac
     2101 ctttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagg gattccaaca
     2161 gttacgagac gcagaagaga cctatccaaa atttgaagaa gtacaagcac gtcagatcgc
     2221 cgattggtta attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg
     2281 cataccagga agcttttcct taggcagagt ccaaacccca acactctata tgatttatca
     2341 attgcaggaa aagattagaa atttcaagaa agaaccttat tttgaaggaa aatccgaagt
     2401 aatcgctcaa aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca
     2461 agaagcattt gaagattatt taaaagaaaa gggggttcaa ttgggaaaac agccaggcat
     2521 gattcgccaa gttgaaaccg agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc
     2581 cagccttcag tctaaaatga atcagctcat gaaagctagt gccaaagaca ctttggaagc
     2641 catgcaaggc ttgtatgaag ggaaatatct aagctatcca cgaacagaca ccccttatat
     2701 tacggaaggg gagtatgcgt atttgttgga tcatttagat gaatacaagc attttttaaa
     2761 ggccgaatca atcccaaccc ctattcatac gccgaatagt cggtatgtga ataacaagaa
     2821 agtccaggaa cattacgcga tcattcctac aaaaacggtt atgacagcag cggcttttga
     2881 acaactaagt ccactgcagc aggctattta tgaacaagtg ttaaaaacaa cggtggccat
     2941 gtttgcggaa aagtatacct atgaagaaac aacgatcttg acgcaagttc agcagcttca
     3001 gttaaaggca atcgaaaagg tgccgctaga tctaggttgg aagaagttat ttggaaaaga
     3061 gagcgacgga aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat
     3121 ggtgacggtg gatctacaag ttcttgaaaa agaaaccaag ccaccacaac cttataccga
     3181 aggtacgttg atcactgcaa tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca
     3241 atccatattg aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga
     3301 aaccttgaaa caaaaggaat atatcaaggt agagaaaaat aagctcgtgg tgacgaataa
     3361 agggattttg ctttgccaag ccgtggaaaa agagccgctg cttacgagcg ccgaaatgac
     3421 cgccaaatgg gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt
     3481 tctagccaat atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca
     3541 atccactgat tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc
     3601 cgcgcgtcat ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa
     3661 ggtggcagct tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag
     3721 caacgattgt tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa
     3781 cgaacttcaa gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta
     3841 ataccacaca caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg
     3901 aaaaaatgcg tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa
     3961 aagaaatcga attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt
     4021 atactgtttt tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt
     4081 atacaagctg ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg
     4141 catttctcct gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc
     4201 aggttttttc accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata
     4261 tgaacgcggc attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat
     4321 ccctagacag atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa
     4381 ggaattcgca gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga
     4441 aataaaaagt cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga
     4501 catttggaat ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta
     4561 tatggagtcg gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac
     4621 agacaaaacg atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc
     4681 tacgcagtta aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct
     4741 tgttttaaga cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg
     4801 tgaaaatttt gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg
     4861 cacaaccaaa gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac
     4921 aacggttcta aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca
     4981 aggaatgaaa gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc
     5041 acatttggaa aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta
     5101 tcctgcaacg atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga
     5161 atcctatcct acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac
     5221 agataattcg agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat
     5281 cgatgagaag accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga
     5341 tccgttaaag atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa
     5401 agatacctgg aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga
     5461 gacaatggat tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg
     5521 aaaactagct tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt
     5581 aataatgtga cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt
     5641 ggtactgcag tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc
     5701 aattgagaag ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg
     5761 acattagcta aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac
     5821 gacaaccagc aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta
     5881 ttagaaagta aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca
     5941 agcaccgcca cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct
     6001 tcacagaatc catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag
     6061 tttgagaaag aaggagtaag catggcaaaa aaaattagtc tgacgatgga tctattgata
     6121 cccgtaattg catttataga cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat
     6181 agaattgcga aaggatcact atttcttgta ttcgtgtatc ttgtctgttc ttatctttta
     6241 aaaagagcag gaaaggataa ttccttttgg cgattgacta aagtcacagt agcagcgaca
     6301 gctgttatac ttacggctat tctaccgctt tatatgtaga gttaacggga aatcgttgaa
     6361 cttcttgcga aatattgcta taatgaacat gaaaaagagg agttgcgcta acaacccctc
     6421 attaacagca ccgattaaag aacggtggca gaatattagt tatttggaaa taaccgctga
     6481 cctgctaaag tagacggtta tttctttttg tcatttttaa gcatatttac aatcaaacta
     6541 atcaaagcaa tggtaaacat accaaaacca agaatggttt gaattgtctc atatgcggac
     6601 aaaaggctta ctcctttcat agattttagc acttacattc ataagcacca ccacctttcc
     6661 agaagtcgcc accgtcttca aactttactg tcttgactat tatagcatat ctaaaatcga
     6721 ataaaaattc tcaattttag ctgtttttgc tataatttta gcgaggtgat cgtgatggaa
     6781 caatatattg gtttactcga taaattaggg tgatcaagac acagccattg cttgtgcgat
     6841 ttacattacg cacgagtcat gaaccaatca attgtgtagt ggccaatata gaaatagcgg
     6901 ataaattgct gattttggaa gaaggcaagt atactatttc agtgacaggt cacttcaata
     6961 aacgaaatca actagtcatt gaatcaatgc aaattagaaa ccctgatcat tttacgagat
     7021 taatgggcat ataaataaaa gaagagctgc agtcctaaat gaggattgca gctctttttg
     7081 ttttacatag aaacaagaaa aatgaaaagg cctttttagg aaggcgcaag cgccttctca
     7141 attgaaaaac ctctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     7201 gttgcacgtt cactaagata aggggtcttg caaagcaaga caaaatagaa aaaacttcct
     7261 ctcaccaaaa ggttcgttcc tgttcgctat ctctttattg atttgccaga ccggcaacaa
     7321 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcacgccctt
     7381 gtccttgtcg cttttagttg attcattctg gccaacaaat caataaagaa agaactaacg
     7441 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     7501 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccatat actgtcgaat
     7561 gcgattgtgg agaaagagcg aaatgtatca aaagctattg tttgtatcaa tgtccaacat
     7621 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     7681 aacgctgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     7741 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     7801 gatagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     7861 tgcaggatat gaactgcatt atcaagaaaa catggaaact tatgaaaacg aatattggtt
     7921 aaccaaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7981 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     8041 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     8101 gattgaatcg ggtgtcgaaa tgaatctcat tgcgacctac tatcgaacgc ttgaagaact
     8161 gaaaaagcaa aatggacaat ggtttttcca agccttacta tgcttggaag caggcgtcaa
     8221 gccaaacact atcaaacctt ctgaatacca agctttagaa ctaacgtatg ggaaatttgt
     8281 cgaaacgaaa aaagccaaga ctgtttcaag tgaatggttg gactacttag aaaatgtcaa
     8341 caagtatggc gcatgttaca tcatgaaaaa ggaggacaac gaaaatgaat aaggtgattt
     8401 atcaactaac aacggaagat gtgtcacact ttttaacaaa caaagaacat ttgaatgaag
     8461 taatcagacg gattcaaaaa gactttagtt gtatggaaga aatgaagtac tttattcaaa
     8521 gagtcgaaga acaattgtat ttggaaagtg aaacgagtca aacgaaggtt acagtcagca
     8581 ataattattt agacgtgtat aaacgtcgtc ctgacacatt aaaaaaagga gcggagcttc
     8641 tacgactaga aacagaaaaa tatatcatga atgtttacct agaaaggagt gaactggtcg
     8701 aaaactttgt cagctacacg atttgcagga aacactatta ctcagaatca gaatattatt
     8761 caatttttga gagcatggaa ttaatggaag agctaaacag caaagatgcg acggtagacg
     8821 attattacct tgtatttact aaatttttga cgtgtgggac ggaatctatc gtgttaatgg
     8881 gaatggatta tgaactcgct gtacccgaag tatttttgag ggaatatgta gaagaagtag
     8941 ataacccagg cgacgactgg tacacgactt taactgattt tttagatacc tacacttacg
     9001 acgatacaga agaaatccaa aatgaacttg accgtaaacg aatgccatat caacgatttg
     9061 aaaggaaaaa aggtcaataa ccatttatac cgaaaagtga gaatgtgtga aataaaaaca
     9121 aaaaaccttg aaattaaggg aatggttctt atttcgcacc aatcgctcca accgcccccg
     9181 ccgcttaggt ggtgcgaaat aagcgagcaa agcccgaaaa cctaagagaa agaaggagat
     9241 ttatatgaac aaaattattc ccttattatt agtcctattt gttggagaaa tacttatgcg
     9301 acttagtgca gctgtacatt taaacctcgc agtatttttg gtaatactaa tgttttgttt
     9361 gatcttttgg cgatttgtat tagccctcgt aagtctacta ttcaagatga ttgcagccat
     9421 atttttcata ggattatttc gatgaaagga gagagtacca atgccaattg caataggaaa
     9481 taaacggtta cccgtgacgt tggatgaaaa gagacaaaaa gaattgcagt aaccaaagca
     9541 gaagtacggc aaaagtgaat ccaggattat gtgtattgcg ttagatttat tgattgcgca
     9601 agaaaaagca ggatttgaca taccagcact caaaaagtga tcgcactttg cgcacgtgtt
     9661 aatacgtgtt ataattattt tgtaaggagg tatagtgcac aatgccactt acgggaaaag
     9721 aaatgcttaa gttattgaaa aaaatggctg ggttgagcga aggcaagagg gatttcatca
     9781 tcatctttat aaggatggtg ttagaatcac tgttcctgtt catgccaatc aagatcttgg
     9841 ccgaggactt gagcgaaaaa ttttaaagga tgcgggattg aaataatctc gcattcgatt
     9901 aaagaaagga ttgactgaaa tgttaagtga gcctttagca aaaacctatc ctgctatttt
     9961 tagcccagaa gaaggtggtg ggtactttat tgaatttcct gatgttcaag gtgcttacac
    10021 aggaattaac gaagacgata tatcttatgg aattgctatg gcacaagagg tgttaggtat
    10081 ggttttagca gattatattg agcatgaaga tctacttcca gaaccaacac caatcaataa
    10141 gatttctgtt gaagatgatt cttttactac tttaattcgt gtagatgttg ccaaatattt
    10201 gaaagacaca gaacttgtca aaaaaacctt aacaattcct caatgggcgg ataagctagg
    10261 caaaagggct ggcataaatt tttcagtcct tctaacagaa tcaattgcag ataaagcaga
    10321 taacattctt cgttctggaa gaaataatta gaactcaaaa gggtcagaaa cttaaaaatc
    10381 tttggcttct ttttttacta caaagatggc ttctttaaat tatttgcctg attgatttaa
    10441 aaaaattgcg caaagactac gagctaatta agttgctcgt ggtctttttt tatgaaaact
    10501 ttttagatct tattatttgt caaattaagc gagacaaaac tttccattta cgtaactcaa
    10561 aaaaacttct aatggtgttt ggtaatttag tgattttcta gggatatggt ttcttttgga
    10621 cgcaacggat gagatgaatc cttgattgac ttggttgaag tccatttctt ttggtagtcc
    10681 atcttttcgg aggagtccgt ttgaatgttc atttaatccc cgttgggaag gcgttcctgg
    10741 gtctgagaaa taaatatcaa tatcattcgt attactaata cttttccaac tagagaattc
    10801 ttttccacaa tcaaaggtaa ttgatttgaa aagatttttg ggtacggatt gaagccattc
    10861 attaatcgaa ttttcaatat caact
//
LOCUS       DAYYHB010000084        10762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000084 DAYYHB010000000
VERSION     DAYYHB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.086"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1822)
                     /gene="pyk"
                     /locus_tag="ACHA6B_002173"
     CDS             complement(38..1822)
                     /gene="pyk"
                     /locus_tag="ACHA6B_002173"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8949724.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1896..2858)
                     /gene="pfkA"
                     /locus_tag="ACHA6B_002174"
     CDS             complement(1896..2858)
                     /gene="pfkA"
                     /locus_tag="ACHA6B_002174"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8949725.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3026..6340)
                     /gene="dnaE"
                     /locus_tag="ACHA6B_002175"
     CDS             complement(3026..6340)
                     /gene="dnaE"
                     /locus_tag="ACHA6B_002175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8949726.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            6571..6756
                     /locus_tag="ACHA6B_002176"
     CDS             6571..6756
                     /locus_tag="ACHA6B_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8949727.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6818..8014)
                     /locus_tag="ACHA6B_002177"
     CDS             complement(6818..8014)
                     /locus_tag="ACHA6B_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8949728.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8184..9620)
                     /locus_tag="ACHA6B_002178"
     CDS             complement(8184..9620)
                     /locus_tag="ACHA6B_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8949729.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9913..10722
                     /locus_tag="ACHA6B_002179"
     CDS             9913..10722
                     /locus_tag="ACHA6B_002179"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8949730.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3215 a         2250 c         1922 g         3375 t
ORIGIN      
        1 taaaagaaga gctgttttta ttttttatta agctaaatta gatagctgtt gtttcgccac
       61 ggtaaacgat gccgcgacgt gaatcaacgg taatcaattc accttctgta attgttgttg
      121 ttgcgccttt agcaccaacg attacaggaa tatcttgtgc aattcctaca actgcagcgt
      181 gagaagtcaa tccgccttct tcaacgataa cagcagctgc tttttcgatt gcaggcatat
      241 attctttgtc agttgatggt acaactagaa ccattccgtc ttttgctttt tttacagctt
      301 cttcagcaga tgaagcaata actgcatttg caacgacaga tttttgtcca acgccttgag
      361 cttctaataa tttagaacca atcatttgaa ttttcatcac gttagttgtt ccgcgttcac
      421 caactggtac accagcagtg atcaagatta aatcgccttc tgatgcgaaa cctaattcaa
      481 cagctttttt agctgctagt tcaaacattt catcagttgt tgatggtttt tcagcaactg
      541 ttgggtaaac gccccagttt aacatcaaac cacgttttgt gcgttcgtca aaagtaacag
      601 ccaagatatc tgcatctgga cgatattttg aaatcatttt tgcagtgtat cctgattcag
      661 ttgcagcaac gattgttttc acgcctagat tttttgcagc acgagctaca gataaaccga
      721 ttgtttcagt tacatctgtt ttatcgaatt cgttcaattg gaatgtacct aattctgata
      781 atgcattttc agcttcttgg tcgatacgtg ccattgttga aacagcttca actgggtagt
      841 ccccgtttgc agattcacca gatagcattg ttgcatctgt gccatcaaat acagcgttag
      901 caacgtcaga agcttccgca cgtgttggac gtgggttttc ttgcattgat tctaacattt
      961 gtgtagcagt gataactggg ataccagcag cattacattt tttgataatg cgtttttgaa
     1021 ccattggtac tagttcagct gggatttcaa cacccatatc tccgcgggca atcataacac
     1081 cgtcagaaac tttgatgatt tcgtcaatgt tgtcgatacc ttcttgagat tcgattttag
     1141 ggaagatttg aacatgagtc atgtcttttt cttctaagat ttcgcggata tctagaacgt
     1201 cttgtgcttt acgtacaaaa cttgcagcga taaagtcgat atcgttgtct aaaccaaaac
     1261 gaatatcgtc agcatctttt ggagtaatac ctggcaaact aatagaaacg cctggtgcat
     1321 ttaccccttt acgtgatcct agcatacctg catttttaac ttctacgact aattcacggt
     1381 ttgcttcgtc tttttcaatg atttgcatat cgatcaaacc atcatcaaat aaaatgtgtc
     1441 cgccaacgtg aacatcgtca aataatcctg ggtaagtaac cgcgattttt tcttttgaac
     1501 ctacatgttc aggatccata gagatgcgtg tttgatctcc tacttcaaat ttgatgtatc
     1561 ctgcacgacc aaaatcagct tctgttgtgc cttgaacagt tgtacggatt tcagctcctt
     1621 ttgtatctaa aagaataccg acatcttttc ctgtcttttt aactgcttca cggaccatag
     1681 tcatacgtga taattgttct tcatggtcac catgagaaaa attaaagcgg aagacatttg
     1741 ccccagattc aatcaattga gaaataattt ctactgagtt acttgctggt cctaatgtac
     1801 taacgatttt ggtttttttc atttaaaaaa ctctcccatt caattttgtt tatttaaagc
     1861 tgtacacagc ttaaatagct tggtttcttt cactcttaaa atgaaagtga attatttaga
     1921 tcatatagtg ataagtctgg tttatgtttg ttgttttcaa gtgtatcaat gatgtcagac
     1981 gcaacgattt cgttgtcatg gataccgaca cataatccac cttttccttc ttgaagcaat
     2041 tcgacagcgt aagcaccgaa tttacttgcc aacacgcggt cgcgtgcgct tggtgctcca
     2101 ccacggacaa cgtgtcccaa gatagataca cgtgtatgat agtcaccaaa ttctgaaagt
     2161 ttctcagcga attcatttcc gcccattaca ccttcagcca agatgatcaa gcaatgtttc
     2221 ttcccgcgat cgcggccttc ttggattttt ttagcaactg aagccatatc aaaatcatgt
     2281 tctgggatga tgatttcatc cgctccgcca gccacacctg accataaagc gatgtctcct
     2341 gcattgcgtc ccattacttc gataacgaat gtacgaacat gagaagtcgc tgtgtcacga
     2401 atacggtcaa ttgattctag tactgtattg attgctgtat caaaaccgat tgtgaaatca
     2461 gtcccaggaa tatcattatc gattgttcca ggaataccta ctgctgggaa accgcgtttt
     2521 gtcaaagcca ttgcgccatg ataagagccg tctcctccga taaccactag accttcgatt
     2581 ccgaattttt tcaattgttc gattcctttt aattgacctt cttcagttgc aaattctgga
     2641 taacgtgcag aatacaagaa tgtaccacca cgttggatct tgtcacctac atctgccaca
     2701 tcaagacgac gaatatctcc agcaactaaa ccggcaaaac cataattgat cccgtatact
     2761 tccagtcctt cgtaaatacc tttacgaaca acggcacgaa cagctgcatt cattccaggt
     2821 gcgtctccgc cactggtcaa aattccgatg cgtttcataa taaacattca cctcattaaa
     2881 atactttatc ctaagtatga cttaggttta caaccatttt tgcgttaacc acgactaaca
     2941 ttctaacatt atttttctca cttgtcttga tttacggata aaaaaatgaa aaaaaatgaa
     3001 agcaagtagt attttttacc gtaagttatt tgaaaaccac attatcttcg ccaagcagat
     3061 aaataagttg ccgctttata ctcttgctgt catccaccca ataagcatct gacagaacta
     3121 cttttcgttg cgttttttcg tgatacataa ctactggtat atatcctggg tgtttctgaa
     3181 aaatcacttg taattgtttc attatctcgg gatcttcccg ttcttctgta atacgcaaat
     3241 agcaagtttg tgaaggatac tgggcttcta actggttagg atcagcaaag ctttctccaa
     3301 ttagttgcag ttcctgatta tatttgctga tttcgctccg cccttgaaca aagatcactc
     3361 gatcaacggc aaacaaatta cgatatttac gataaagagt tggaaaaact gtgacagaaa
     3421 tttctccact actgtcggtt ccttcaacaa aggccatcag ttctcctttt tttgtccgaa
     3481 tttctctaac ttccttgatc atcactaata aattgactgg ttgattaggg acgatatctg
     3541 taatcagctg aacttgttta gctaaacgaa gacgatcaaa cccttctgca ggatgacctg
     3601 acaaataaat acctaagtac tcctcttcca gcttcaagcg ttcttctagt gtatagtcag
     3661 cgacttcttc ttcttttaat gccatgattc ccagcaagtc aagacttcca ccactgtaaa
     3721 cgatattttg gatttttcct tcgagatcaa gcatcaattg tttcctgtta ggagataatt
     3781 catcaaaaac tcctactgct gtcagtgatt gcagtaactc taatttcagc caacgcttat
     3841 cgatccgtat gaggaattga tccactgact ggaacgggcc attttgtttt ctttcgttta
     3901 atatatcttc tacaaaatca cgccgaatac cttttatcgc tgtcagtccg aatctgattt
     3961 cttccttatt gaccaactca aaactggaat tactttgatt aatggacggc gaggaaagtt
     4021 tgagtttatt tttacgtgct tctgcaatat attctttcaa ttttgatgtg ttatgccgaa
     4081 cagaattcat caggctggta aagaaagcac ctggatgatg ggcttttaga tacgccattt
     4141 ggaatcccac gaatgaataa gcaaatgcat gtgaccgatt gaatccatag tttgcaaatt
     4201 tttcgatata ttcatatacg gtcgctgctg ttttttccga atatccctgt tgtaatgctc
     4261 ctgatacaaa atgcgacttt tcttcatcta atacgtcttt tttcttttta ctcactgccc
     4321 gtcgcagaat atctgactgc cctaatgtaa atccagccat agtcgaggcg atctgcatga
     4381 tttgttcctg atagacgatc actccatatg tgtttttcaa aatcgggatc agtgaatcat
     4441 ctgggtagcg aatggcttct tttcctttct tcctggcaat gaaggtatct atattttgca
     4501 tagggcctgg tcgatacaat gcattgactg cggcaatatc ttcgatgttt tctggatcta
     4561 atcttcgtaa aacgttccgg ataccagcag attcaaattg aaatactcct gaggtttcgc
     4621 ctcgttgaaa caagagcagc gtttttgcat catttagcgg gatctctttt aatcggatct
     4681 catacccttc tactcgctta acagctttca aagtaaaatc aataatcgat aagttgcgta
     4741 gtcctaaaaa gtccattttc aaaagaccga ttgcttctac ctcattcatc gtatactggg
     4801 tcagaaaagc ttcatcagaa ccgcgttgga gcggaactag ttcaagtaga ttcttatcgc
     4861 tgataactac acctgctgcg tgagtcgaaa catgtcttgg caatccttct aaaatcactg
     4921 cagttttgaa taaaagttgg tttttttcgc ttgcctgaac taattctact aattgatgcg
     4981 aagttttata agcttgggtc aatgtgattt tcaattgatt gggtattgct tttgaccagc
     5041 ggtttgcttc actttgcgat aaaccaaaca ctctagctgc atctctaaga accatttttg
     5101 ctgccattgt gccaaatgtt gcgatttgtg ccatatgata ttgtccatac ttttctcgaa
     5161 cataattcaa tacttcttca cgtcgattgt caggaatatc caaatcgata tctggcatcg
     5221 taaagcgttc ggggtttaaa aagcgttcga acagcaaatc atattctaat ggatccacat
     5281 ctgtgatttc taacacatag gctactaatg aacccgcagc agaaccacga cctgcaccgg
     5341 taacgatttt gtttctatga gcaaaagcca ttacatccca tacgatcaag aaataatcat
     5401 cgaatcccat ttgatggatg acagataact cgtagtccaa acgttcttgg tattcctttg
     5461 tatagtcagt gattcggcgt tccacttgcg aatagcataa ctctcttaaa tattctccag
     5521 cttgttttcc attaggaact ggataatgcg gaagtaattt ctgatggaac tggaattcaa
     5581 atgaaatcga ttcagctaaa cgctgggcgc ctattacagc ttgggaaaga cccgcttttt
     5641 cgaatcgttt gatcatttct tgttctgaaa gaagatagac atttgctgtt agtggtaaag
     5701 atgcagataa attttctaaa ggtatttggc ttccctcttt gatatgtttc atcacttcaa
     5761 gtgcaaaaag ttcttcttgt tgtaaactag cgatgggatg taatgcacaa agcgaataat
     5821 tttcttttat cacatactga taccattcag gcaattgatc aggcgtgttt tctgtcaagc
     5881 cgatataaaa atttctagta aaaacatttt caagtaaaaa ctgccaatga ccgaaaaagt
     5941 tatcctcttt attgaaagcc tcagaaacag aatgatttgc cggaacaaca aacgctagat
     6001 ggtcggtttc tgtcatttct gcaagttcca atttttcttg gatcattttt cggctggaaa
     6061 gtttcatcag ctgctgataa cctatttcat cttttgcgta cgcaagcaag ccataatttt
     6121 tttcttcata agtataacta agatgtaatc caatgattgg ctgtactcct tcttctacac
     6181 aaatacgata gaattcaggt gcaccgtata agacatcttt atccgttatg gctaatgaag
     6241 tgtagccttg tttttttgct tcttgtacat attctcgaat acgtatcgta ctttgtaaaa
     6301 gagaataaga agtaattgta taaagttgtg ggaaaaacat cttgcattcc gccttttttt
     6361 tcgttaaaat tcactagcgt taaaaggttt gatcctttta ctttcattaa gatagcaggc
     6421 gctgaagttg attattccac ttcatttgct tgcttgtaat ctgtaggaat gagttctttc
     6481 gcaagaactc aaattttatt gagatttggt ctcgtttctt tacattcaaa gaaaatctgg
     6541 taaaattaag atagaaaaga ggtgttcttt atgcgatata tcgttacatt attctgggct
     6601 gtcgttctag gtcaagtagt cggttatatt ggtgctgcat taacaagcgg aacatatgat
     6661 tttactttga caacaatcat ttcattcatt gcgggtgtca ttatcctttt gattggtgca
     6721 gttgcgccaa gaaaagaaac ctctgcccat tcgtaaaaaa acagccaaga gcttgagatt
     6781 tctcaagctc ttttttattt tctactcctc accaccatta taatctgctg cgaagaactt
     6841 tacgaaagtt gaataaagaa agaagaatat ttgacagaac aaacaaactt gtcacaataa
     6901 aaacagcact gtaattataa gcatgcgcga ctgtcgatcc tagcatggga cctaaaactt
     6961 gtccaaagtt gctaaacatc tgattgtagc tatagattcg gctgactcct tcttgaggcg
     7021 taatcttact gatcagtgta ttgacagaag gcatcaaagc gccagttgaa aagcctaaaa
     7081 taaagcgaag tatacctaat tggagcggtg tagtgacgaa tgccataggt aaataacagc
     7141 aaaatgataa aatgagtcct ccaattagaa ctttttgatt cccaatctta tcccctagtt
     7201 ttcctagttt cggtgaagaa aagacagcgg aaacaccagc tatcgaaaca atcaagccgc
     7261 tgacaaaaag gatattttct gtacttccgc tcaattcacg aatatataag gtcaaaatag
     7321 ggctgatcgt agtgattcct atctgtaaaa tcaaggtcgt aatgaagaga ccgatcaaaa
     7381 tagagagatg atccattttt gaaaaaattt ctttggtgct tatcagatct tttttctcga
     7441 ttggatggaa atcttctttg actaagaaaa tggtcaatac cgttgtgatc atcaacaaag
     7501 cccctgtgat caagaataca ttttccatac caaaccactg agccagtgca ccgcccattg
     7561 aaggtccgat cagatttcca gcaatcgctc cggtagacaa tgttcctaac gcccatccgc
     7621 tcttttctct aggtgcctgt gaagcaatca tcgctgtggc atttggaata tatccagaca
     7681 aaatcccatt ccaaaaccgc atgatcaaaa gccaatacgc gtttggtaca aaagccaaag
     7741 atcccattgt taatgtcatc cctgctgctg cacggatcat catgagtttt cggccttttc
     7801 gatcagctag atttccccag attggtgcaa ccacggcaga tgcaaaagca gtcacagaga
     7861 ttgctagtcc cgaaaacaat tcgatttgat cttttggtgt tcctagttgc tcaacataaa
     7921 ctggtatgaa tggcataact aaactaatac ttgcaccagt gaaaaaacag ccaatccacg
     7981 caatcactaa attttttctc caattaatct ccatctatca tccctctttc gtctctttta
     8041 tcatacctat tttcgaataa acaagcaacg atctcgccag taaaaagaga aaaatcccgc
     8101 aaaggtcatt ttatatgtaa cgttcttttt atgtaaaaca aaaagagatc agaacaaata
     8161 ctgttccaat cccttctctt tagttaaatt gttgctagaa tgatgaagtt gataatgaat
     8221 aaaatatcaa cgatccacaa agcaacagaa acttgattgt attttccttt gattactttc
     8281 acaattgtat agaaaataaa tcctgctgca atcccatagg aaatgctata gcacaatccc
     8341 ataaagatcg atgcaaagaa ggccggtaaa gcttcttcca tatctagcca tttgatgtct
     8401 gcaaaagaag ccatcatcat tactccaact aaaatcaatg ctggcgcagt tgcttgattt
     8461 ggtacaatgg cgatcagtgg agagaataaa ctacttaatg cgaatagcac tgccactact
     8521 actgaagtca aaccagtacg tccaccggcc ccgatcccag ctgcagattc gacataagtt
     8581 gttgtattcg atgtaccaaa gatagccccc acagaagtag cgatcgcatc ggcaaatagt
     8641 gctttatcca tttttgtttt gaacccacga ccatcttcca acgcgatctc gtcttctttt
     8701 gagaaaatac ctgtgcggcg tcctgtaccg ataaatgttc cgatcgtatc gaaagtatca
     8761 gataaactga atgcgatgat cgtcatcaat acttgaggaa tcttggaaga atcactgaat
     8821 aaagattgca tgccttcaga accaaatgct gcaccgaaag ttgttcctaa ttctttaaat
     8881 gaattaccta acgaattcgt ttgccaatca atggaactta aatcaacaac acccataaaa
     8941 ataccaagga ctgtggtcgc tacgataccg ataagaatag ccccacggac attttttaca
     9001 actaaaatgg ctgtcagtac caaaccaatc acagccaata agataggagc gttgtcaaag
     9061 ttggctaacg cagggacgat tccaccattt atcgtaacat ttgtcgcttt tccgccttca
     9121 acgacagagc tcgaaatagc tgactggtct gctgaaaaag ataagaatcc tgcatttttg
     9181 attccgacat acgcaacgaa aataccgatc ccgccaccaa tcgcatgttg aagactttcg
     9241 ggaatcgccc gaataatcat ttttcgaata tttgtgacag taataaaaat attgattaat
     9301 ccgcaaataa acaccattgc caaggcttgt tgccaagaat aaccaagccc aaaaacgacc
     9361 gtaaaagtga aaaatgcgtt caatcccata ccaggtgcct gagcatatgg tacatttgcg
     9421 aacaatccca ttaccagcgt accaataatc gatgcaatga ttgtcgctag aaatactgct
     9481 tggaagggca tacctgatgc tgacaaaatc gtcggattta cgaataagat ataactcatg
     9541 gcaaagaatg tcgttactcc cgccattatt tcagttgata catttgtatt gtttttcttt
     9601 aaaccgaaaa acttttccat aaaaagttcc actccctgaa tttttttctg atggaaaact
     9661 ataataataa aaaataagta aaatagggca aatccatcga acaatttcaa attatataga
     9721 cgtttcattc gcttttcaac gtttttttaa caaaaaaagc gaaaataata cttaacgttc
     9781 gtgtttagct tatgtttagc tatttatata gccataactg tagtttttgt taggaatagc
     9841 tgattgctct tgttaattac ctaaaatatg ctagactaaa acaaatgcta gttgaggaag
     9901 gaaggaccct tattgaataa aaaattaatt gccatcgatc tggacggaac taccctcaat
     9961 caagattcga tgattacatc taaaacaaaa gagactctaa aaaaagcaat caatgccggt
    10021 catcatgtca gtattgctac aggacgccca ttccgaatga gccatcagtt ttaccaacaa
    10081 ttagaattaa caacaccgat ggttaatttt aacggcgcat tagtacacat ccctaatcag
    10141 cactgggatg gtgaaagaga aacgttgatc aatcgtgaaa tcgcttttga aatcttatcg
    10201 caaaaaaagc agctaaattt agattttatc gctgctgaaa atcgtgatac tttttttatt
    10261 gacagttttg atttctttga tgaaaaaatt tttgcttctt ctcgtccagg agagaagaat
    10321 ttgctttctc ctaaaaattt gacgacaaat cctacatctt tgcttgtccg aacagacaaa
    10381 cgttttgcag gagctgtatc tgctgaatta acacgtcaat ttggcagtta tgtcgatgtt
    10441 cggacatggg gtggacctac tgccatctta gagattgttt caaaaggaat ccaaaaagca
    10501 aaaggcgtgc aagagattgc taactacctg tctatcgatc aaaaagacgt cattgccttt
    10561 ggtgacgagc ataatgattt ggaattgttg gattatgcag gttggggagt agctatggct
    10621 aacggtactg accaactcaa aggcattgca aatgatgtca ctcctttgag caatcaggaa
    10681 gacggtttag ctgtttatct ggaaaaacta cttaaactat aatagagaat tagattccac
    10741 aaaaaagagg ttgtgacaat tt
//
LOCUS       DAYYHB010000085        10761 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000085 DAYYHB010000000
VERSION     DAYYHB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.087"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..310)
                     /locus_tag="ACHA6B_002180"
     CDS             complement(32..310)
                     /locus_tag="ACHA6B_002180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949731.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACHA6B_002181"
     CDS             complement(425..1042)
                     /locus_tag="ACHA6B_002181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949732.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACHA6B_002182"
     CDS             complement(1437..2261)
                     /locus_tag="ACHA6B_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8949733.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACHA6B_002183"
     CDS             2604..3020
                     /locus_tag="ACHA6B_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8949734.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACHA6B_002184"
     CDS             3017..3487
                     /locus_tag="ACHA6B_002184"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949735.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACHA6B_002185"
     CDS             3501..4274
                     /locus_tag="ACHA6B_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8949736.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACHA6B_002186"
     CDS             4288..5100
                     /locus_tag="ACHA6B_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8949737.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACHA6B_002187"
     CDS             5154..6119
                     /locus_tag="ACHA6B_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8949738.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="ACHA6B_002188"
     CDS             6256..8979
                     /locus_tag="ACHA6B_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8949739.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="ACHA6B_002189"
     CDS             8992..9624
                     /locus_tag="ACHA6B_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8949740.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9832..10056
                     /locus_tag="ACHA6B_002190"
     CDS             9832..10056
                     /locus_tag="ACHA6B_002190"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8949741.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10158..10391
                     /gene="relB"
                     /locus_tag="ACHA6B_002191"
     CDS             10158..10391
                     /gene="relB"
                     /locus_tag="ACHA6B_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8949742.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="ACHA6B_002192"
     CDS             10381..10650
                     /locus_tag="ACHA6B_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8949743.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3567 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt a
//
LOCUS       DAYYHB010000086        10481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000086 DAYYHB010000000
VERSION     DAYYHB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.088"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..1689
                     /locus_tag="ACHA6B_002193"
     CDS             229..1689
                     /locus_tag="ACHA6B_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8949744.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1700..3904
                     /locus_tag="ACHA6B_002194"
     CDS             1700..3904
                     /locus_tag="ACHA6B_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8949745.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3920..4069
                     /locus_tag="ACHA6B_002195"
     CDS             3920..4069
                     /locus_tag="ACHA6B_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949746.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4145..4738
                     /locus_tag="ACHA6B_002196"
     CDS             4145..4738
                     /locus_tag="ACHA6B_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8949747.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4751..5650
                     /locus_tag="ACHA6B_002197"
     CDS             4751..5650
                     /locus_tag="ACHA6B_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8949748.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            5653..6138
                     /gene="ssb"
                     /locus_tag="ACHA6B_002198"
     CDS             5653..6138
                     /gene="ssb"
                     /locus_tag="ACHA6B_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8949749.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(6279..6383)
                     /locus_tag="ACHA6B_002199"
     CDS             complement(6279..6383)
                     /locus_tag="ACHA6B_002199"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8949750.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            6557..6817
                     /locus_tag="ACHA6B_002200"
     CDS             6557..6817
                     /locus_tag="ACHA6B_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949751.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7254..7460
                     /locus_tag="ACHA6B_002201"
     CDS             7254..7460
                     /locus_tag="ACHA6B_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8949752.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            7460..7711
                     /locus_tag="ACHA6B_002202"
     CDS             7460..7711
                     /locus_tag="ACHA6B_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949753.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            7726..8163
                     /locus_tag="ACHA6B_002203"
     CDS             7726..8163
                     /locus_tag="ACHA6B_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949754.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            8156..8863
                     /locus_tag="ACHA6B_002204"
     CDS             8156..8863
                     /locus_tag="ACHA6B_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949755.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            9028..9228
                     /locus_tag="ACHA6B_002205"
     CDS             9028..9228
                     /locus_tag="ACHA6B_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949756.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            9244..9423
                     /locus_tag="ACHA6B_002206"
     CDS             9244..9423
                     /locus_tag="ACHA6B_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949757.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            9554..9823
                     /locus_tag="ACHA6B_002207"
     CDS             9554..9823
                     /locus_tag="ACHA6B_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8949758.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            9816..10073
                     /locus_tag="ACHA6B_002208"
     CDS             9816..10073
                     /locus_tag="ACHA6B_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8949759.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT         3894 a         1718 c         2120 g         2749 t
ORIGIN      
        1 attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt
       61 gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa
      121 aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa
      181 agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa
      241 aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat
      301 ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt
      361 tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt
      421 gaattagcta aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa
      481 gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga aaatcttcat
      541 tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga
      601 aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa
      661 gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt agttcgagac
      721 gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa agagacagta
      781 ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag aggcacatat
      841 aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat cggtgaccct
      901 aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc actgaatcgt
      961 gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt
     1021 cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatc
     1081 gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg
     1141 ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat
     1201 tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga agaaaaggtc
     1261 acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa
     1321 ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt taacgattca
     1381 atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaagtttgt
     1441 gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat
     1501 gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa
     1561 catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca tgagatccag
     1621 caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag acaacaagaa
     1681 agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt cacaagcgaa
     1741 agcttatgcc gatagcttct ctaaagccac tcgaaaagac gggtattttg aaatccaaga
     1801 tcgattgttt cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag tcgaattaga
     1861 ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt tgcctatttt
     1921 ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca aggtggtgaa
     1981 acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga
     2041 actgatcgct tggacgatca ttcaacaagt aggcgccgat caaggaaaaa cctttaaaag
     2101 actttggatc aactccttag aaaaagaagc catttatcag ggattccagc agttacgaga
     2161 cgcaaaagag acctatccaa aatttgaaga agcacaagca cgtcagatcg ccgattggtt
     2221 aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag gcataccagg
     2281 aagtttttct ttaggtagag tccaaacccc aacactctat atgatttatc aattgcagga
     2341 aaagattaga aacttccaaa aagaaccata ttttgaagga aaagcccaag taatcgctca
     2401 aaatggcgca tttgatgcga agcttgatcc aaatgaaacc caagcgacac aagaagcatt
     2461 tgaagcctat ttaaaagaaa aaggtgttca attaggaaaa cagccaggta cgatccttca
     2521 agtcgaaaca gagaaaaaaa gtgcggctag cccacgtctt ttttctttat cgagccttca
     2581 gtctaaaatg aatcaactca tgaaagctag tgccaaagat actttggaag ccatgcaagg
     2641 gttgtatgaa ggaaaatatc taagctatcc gcgaacggat accccttata ttacggaagg
     2701 ggaatatgcg tatctgttgg atcacttaga cgaatacaag cattttttaa aagccgaagc
     2761 gattccgact cctattcata caccgaatag tcggtatgtg aataataaga aagtccagga
     2821 acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg aacaactaag
     2881 tccactgcag caggccattt atgaacaagt gttaaaaaca acggtggcca tgtttgcgga
     2941 aaaatatacc tatgaagaaa caacgatctt gacgcaagtc cagcagcttc agttaaaggc
     3001 aatcggaaaa gtaccgatag atctaggttg gaagaagtta tttggcaaag agaacgacgg
     3061 aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa tggtgacggt
     3121 agatctacaa gtccttgaaa aagaaaccaa gccaccacag ccttataccg aaggtacgtt
     3181 gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc aatccatatt
     3241 gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg aaaccttgaa
     3301 acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata aagggatttt
     3361 gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga ccgccaaatg
     3421 ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt ttctagccaa
     3481 tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc aatccactga
     3541 ttttggatcg acgttacaag aagtgaaagc agccagtgaa aaacaggaag caacgcgtca
     3601 tttaggtgtt tgtccaaaat gtcgggaaca agaagtcttg ctttatcaaa atgtggcggc
     3661 atgtacgtct gaagcctgtg attttaaatt atggacaacg attgccaaaa agaaactcac
     3721 agccacacaa ctgaaagaga tcattcaaaa cggccgaaca agccaatcag tcagaggact
     3781 aaaaggtcag aaaggttcgt ttgaagcaac gatcgtttta aaagaagact ttacaacagg
     3841 ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa agaacacgcc gcactacaaa
     3901 ataatgaagg ggttatccta tgcgaaatac aacacacaaa caacaagaaa tcgaaaatca
     3961 aaaagcaaaa attcgtttag aacaagagaa attacgtgca aataaaagaa gactgcaaca
     4021 attaaagaac cagcagcgca ttcaaaaaga aatcgaatta gatcgataga cgttaatctt
     4081 gttacaaata aactactatg atatatttga tgtagtgtat ttgtaacgaa gggaggggaa
     4141 gtaaatgcaa gataaactga aagaattgtt ggctattcgc gatggaaatt tatctatgaa
     4201 ggaagccagg aaatttggaa tcgcagcaac cactgtacaa agattggtga atcaaggaaa
     4261 attgatcaaa gttgatcgtg gttattatgt agaggatggt catggaatag atgatttatt
     4321 ttggcttcag caacgatttt ccaggggaat cttctctcat gaaacgactt tagatattta
     4381 ccagctttct acgaatatgc cgaaattcat tcacttaact tttccccatg gctataacgt
     4441 tgatcgttca attacaaaag agcagcagtt acaatttcat ttcgtgaaga aagaagttta
     4501 tgaactagga aaagtagagg gaacttcctt tcaaggaaat cctattacta tgtatgataa
     4561 agagcgtaca ctttgtgata tttggaatcc gagatatgaa tttgaatatg aaatgaaatt
     4621 agaagcatta aaagagtaca tggaagaccc attgagagat ccaagtaaat taagagatta
     4681 catgacaaaa ctacatgtag aaaaagaaat gaaataccac atgcaaagct tatattgatg
     4741 gaggaaaaag atgacaccga cacaatttgc agcaaaaaca agaaatttag caaagagtac
     4801 aggattaaat gcacagttag tgcaacggca ttttatgatg gatcgtctaa ttgaacaaat
     4861 ttctgaatca aagtacaaaa atgacttcat tttgaaaggc ggctttttat taggatcaaa
     4921 atatggaatc gatagaagga caacaattga tatcgatacg acatttagaa atagcaaatt
     4981 aactgaagaa aaattgaaga gcatttttaa tgatttaact agaaaaccca caaaagaagg
     5041 cattgttttc tctattcaag gaatgaagga aacaagagaa gccgattttt atcctggatt
     5101 tcaaattaaa ttggtggcta atttagagaa agtacgtgta ccgtttaaaa tggacgtgac
     5161 aacaggagat tctatttatc ctgaagtaga tacacactat caccaattaa tgtttgaaga
     5221 taaaaaagtg gagattccag cttatccaac agaacaaatt attgcagaaa agctaagtgc
     5281 tacgttcagc tttggcacag ataattcgag agcaaaagat tactacgact tatttaccat
     5341 tccgaaacta gaaaatatcg atacgaaaga gttatacaaa agtgttcgaa atacatttac
     5401 aaaacgtggg gatacaaaac cgttgtcctt ttattatgaa agagaaatgg ataagatcag
     5461 aggaaatgac tatttggaaa agcagtggga aaaatatcgc tcagttaaca cgtttgcgaa
     5521 ttccatttca tttacagata cagtcaacga aattgatcac ctaatgaaaa gtgtgatcaa
     5581 aattgaaaat caagaaagaa aacggcagtt taaaagatca caacaaatga atcaagaaat
     5641 ggagagataa agatgatcaa taatgtaact ttagtaggac gcttaaccaa agatccagat
     5701 ttacgttata ccgcgagtgg tactgcagta gcgactttta ccctagctgt caaccgaaat
     5761 ttcacgaatc aaaacggcaa tcgagaagct gattttatta actgtgtgat ttggcgcaaa
     5821 ccagcagaaa cgatggcgac attagctaaa aagggcagtt tgatcggcgt tgttggacgg
     5881 attcaaaccc gaacctacga taaccaacaa ggacaacgtg tgtatgtgac agaagttgtg
     5941 gccgataatt tccaactatt agaaagtaaa gcagcgacag aaagccgtgc gcacgctgat
     6001 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatgtagc tacgcctaac
     6061 aacaatggat tgaacgcttc acaaaatcca tttggcggac aatcaatcga tattagtgat
     6121 gatgatttac cattctgatt gtaaaagatc aataaagttg ttataatgga tacgaaaaga
     6181 ggagttgcgc taacaactcc cctaacagca ccgtttaaga cggtgaccag cttaatatta
     6241 tttttgaaaa ataatcgtcg accggctaaa gctgacggtt attttttttg attgttgttt
     6301 acaactgcta agattccaaa aatgattgtt gcgatcaaag aaccgaaact aatcattaat
     6361 cctagcgccg tttcaacagg caaaaggctt ctcctttctg taagattttg gaagtacgca
     6421 catacgcacc acctcacttt caatcggata gccaccgtca caaactttac tgtcttgatc
     6481 attatagcat atctaaaatc gaataaaaat tctcaatttt agctgttttt gctataattt
     6541 tagcgaggtg atcgtgatga acaaatatgt tggtttgctt gataaaatcc gagtaataaa
     6601 aacacaacca ttattggttc gctttacttt acaaacaatt catgaatcga ttaattgtgt
     6661 agttgcagat attgaaatta tcgataaatt gttaattatg gatgatggaa aatacaatat
     6721 tgccgtcaca ggtcatttca ataaacggaa tcaattagtc attgaatcga tgcaaattag
     6781 aaaccccgat cattttacgc gatcgatggg catataaaag aagagctgca gtcctaaatg
     6841 aggattgcag ctctttttgt tttacataga aacaggaaaa gaccttttta ggaagacaca
     6901 agtgccttct caattgaaaa acctttctct cactccaacc gccaccgccg ttcgagaaga
     6961 aaggaagtga acgttgcacg ttcactaaga tatggtgtct tgcaaagcaa gacaaaatag
     7021 aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgct atctctttat tgatttgcca
     7081 gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg
     7141 ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     7201 aaagaactaa cgaaaggaaa taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     7261 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     7321 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat cgtttgtatc
     7381 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatgtt ttgattgatg
     7441 agaaagtaaa gaaacggtga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     7501 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagctt tacttattcc
     7561 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     7621 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacatggaaa cttatgaaat
     7681 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
     7741 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
     7801 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
     7861 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
     7921 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
     7981 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
     8041 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
     8101 gaaaatatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
     8161 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa acaaagaaca
     8221 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
     8281 ctttattcaa agagtggaag aacagttgta tatggaaagt gaaacgagtc aaacgaaggt
     8341 tacagtcagc cataatcgtt tagacgtgta taaacgtcgt cctgacacat taaaaaaagg
     8401 agcggagctt ctacgactag aaacagaaaa atatatcatg aatgtttacc tagaaaggag
     8461 tgagctggtc gaaaactttg tcagctacac gatttgcagg aaacactatt actcagaatc
     8521 agaatattat tcaatttttg agagcatgga attaatggaa gaagaaatgg aagagctaaa
     8581 cagcaaagat gcgacggtag acgattatta ccttgtattt actagatttt tgacgtgtgg
     8641 gacggaatct atcgtgttaa tgggaatgga ttatgaactc actgtacccg aagcattttt
     8701 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     8761 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     8821 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtcaa taaccattta taccgaaaag
     8881 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     8941 accaatcgat ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga
     9001 taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg
     9061 tttgttggcg agatacttat gcgtcatggt gcagctctac atttaaactt cgtagtattc
     9121 ttaggaatac tggtattttg tttgatcttt tggcgctttg tgttaaccct tgtgagtcta
     9181 atattcaagt tgattgcagc catattttta atagggttat ttcgatgaaa ggagagagta
     9241 ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cgttggatga aaagagacaa
     9301 aaagaattgc agcaactaaa gcagaagtac ggcaaaagtg aatccaggat tatgtgtatt
     9361 gcgttagatt tattgattgc ccaagaaaaa gcaggatttg aggtaccagc actcaaaaag
     9421 tgacgtcact ttttatccta aaaactaaaa gtgatagcac ttttaattat aagaagttag
     9481 aatattaatc atttgcttaa ttgtacaata taatgtacaa ttgttttata gaaataaata
     9541 aggggtgaaa ggaatggaag cagtagctta ttcaaatttc cgccaaaatt tacgtagtta
     9601 tatgaaacaa gttaatgagg atgctgaaac acttattgta acaagtaaag atgtagaaga
     9661 tacagttgtt gtattatcaa aaagagatta tgattctatg caagaaacgt tgagaacact
     9721 ttctaataat tacgtcatgg aaaaaattcg tcgaggagat gaacaattct ccaaaggtgc
     9781 atttaaaaca catgacttaa tcgaggttga atctgatgat taaggcttgg tctgatgatg
     9841 cttgggatga ttatctttat tggcatgagc aaggaaacaa aagcaatata aaaaagatta
     9901 acaagttaat aaaagatatc gatcgttccc cctttgctgg attaggaaaa cctgagccat
     9961 taaagcatga tttatctgga aaatggtcca gaagaattac agatgaacat agactgatat
    10021 atagagttga aaatgaaacg atatttattt attctgcaaa agatcactat taaccaatcg
    10081 gaagtaagga aagggtcaga aacttaaaag tttttgatcc ttattttatt taccctagtc
    10141 atttaaaaag ctaatatagc ttagtgttga ttgttattaa tgaatgtgtt tgttacgcgt
    10201 attacggata taaggttagt aaaatcattt ctaaagttga ggaaaagtaa atataaatgg
    10261 cttaaatttc aacaatttga agttgaatag atatgttata atactattgt agtgtgggat
    10321 gttagttact aaaggatgac gcttatatat atgactgaat agaataagca ataggtttaa
    10381 taatctattt taaatttttt gtactagttt tagttaatta gcaaaaacaa caaaaataaa
    10441 cttctcatag aatttagcta aaaattaatg atttatttac a
//
LOCUS       DAYYHB010000087        10127 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000087 DAYYHB010000000
VERSION     DAYYHB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.089"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..281)
                     /locus_tag="ACHA6B_002209"
     CDS             complement(84..281)
                     /locus_tag="ACHA6B_002209"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8949760.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(427..1305)
                     /locus_tag="ACHA6B_002210"
     CDS             complement(427..1305)
                     /locus_tag="ACHA6B_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8949761.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1344..2462)
                     /locus_tag="ACHA6B_002211"
     CDS             complement(1344..2462)
                     /locus_tag="ACHA6B_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8949762.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2459..4558)
                     /locus_tag="ACHA6B_002212"
     CDS             complement(2459..4558)
                     /locus_tag="ACHA6B_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8949763.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4609..5430)
                     /locus_tag="ACHA6B_002213"
     CDS             complement(4609..5430)
                     /locus_tag="ACHA6B_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8949764.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5427..6236)
                     /locus_tag="ACHA6B_002214"
     CDS             complement(5427..6236)
                     /locus_tag="ACHA6B_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8949765.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6257..6751)
                     /locus_tag="ACHA6B_002215"
     CDS             complement(6257..6751)
                     /locus_tag="ACHA6B_002215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8949766.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6763..7263)
                     /locus_tag="ACHA6B_002216"
     CDS             complement(6763..7263)
                     /locus_tag="ACHA6B_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8949767.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7328..10024)
                     /locus_tag="ACHA6B_002217"
     CDS             complement(7328..10024)
                     /locus_tag="ACHA6B_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8949768.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKYIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3075 a         2274 c         1659 g         3119 t
ORIGIN      
        1 tctttttttt tcttgctatc tttttataat cacaactctc tataatagaa ttacgattta
       61 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
      121 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      181 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      241 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      301 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      361 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      421 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      481 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      541 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      601 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      661 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      721 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      781 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      841 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      901 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      961 atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata
     1021 ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat
     1081 atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat
     1141 gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac
     1201 tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc
     1261 acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct
     1321 tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt
     1381 ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc
     1441 cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg
     1501 gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg
     1561 tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct
     1621 ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt
     1681 ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt
     1741 gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc
     1801 caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc
     1861 tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa
     1921 tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta
     1981 taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt
     2041 tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac
     2101 gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca
     2161 acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa
     2221 ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca
     2281 gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg
     2341 cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt
     2401 tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc
     2461 atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg
     2521 tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac
     2581 attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat
     2641 caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca
     2701 ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga
     2761 cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg
     2821 ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc agctcgctca
     2881 ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt
     2941 tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat
     3001 ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt
     3061 ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta
     3121 acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat
     3181 caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa
     3241 ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta
     3301 gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg
     3361 tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt
     3421 cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct
     3481 gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta
     3541 acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag
     3601 gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa
     3661 acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag
     3721 gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa
     3781 agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct
     3841 gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt
     3901 cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg
     3961 ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct
     4021 gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt
     4081 gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta
     4141 tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca
     4201 aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa
     4261 tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc
     4321 aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg
     4381 tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct
     4441 ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt
     4501 ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct
     4561 tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc
     4621 aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac
     4681 ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa
     4741 aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa
     4801 aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat
     4861 actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt
     4921 agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt
     4981 gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc
     5041 tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa
     5101 tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc
     5161 agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt
     5221 cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta
     5281 gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg
     5341 attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc
     5401 atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc
     5461 gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga
     5521 tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt
     5581 aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc
     5641 caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt
     5701 aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca
     5761 aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag
     5821 aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca
     5881 gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct
     5941 agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct
     6001 gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca
     6061 cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc
     6121 aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt
     6181 tcatgtccag caactccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt
     6241 tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg
     6301 ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa
     6361 tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg
     6421 tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa
     6481 cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg
     6541 gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg
     6601 gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta
     6661 ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta
     6721 atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc
     6781 ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt
     6841 taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag
     6901 aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt
     6961 ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg
     7021 ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata
     7081 agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa
     7141 gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt
     7201 ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat
     7261 cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt
     7321 agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa agctcataag
     7381 cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta gaaacataat
     7441 aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt tctgtccgag
     7501 ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt tttacacggc
     7561 catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc acttcactca
     7621 tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac gaaaagaatt
     7681 ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga ctcaaaatat
     7741 gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata ttaggaattg
     7801 aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca aaataagttt
     7861 catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg
     7921 attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg
     7981 acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat tgaacattga
     8041 ttgaatactt gtctgctgca tcctccgcaa tatatttaaa tggcgcattc atcgtcatct
     8101 tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt aactcccctt
     8161 ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct acgatcagca
     8221 ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg acaatctctg
     8281 acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg cataattgat
     8341 tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac aggcccttca
     8401 gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt cttagaaagt
     8461 tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat aagtaagtcg
     8521 tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc ttatagcttg
     8581 gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc gtcaatccgt
     8641 acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc aattttctac
     8701 tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact tcattcttta
     8761 tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt ttttccaaaa
     8821 tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt ttctttatca
     8881 ataaactagc aatcggcaag ctttctactt cggattccag catatttaac tgaatcaaga
     8941 tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca tccgcacaac
     9001 tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga gataacatta
     9061 aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct tcattttgaa
     9121 aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa gttggcaaca
     9181 taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca cctttttccg
     9241 tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca ccgactggac
     9301 gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc gataggcgat
     9361 ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca tcggtatcct
     9421 tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa agcagttcgg
     9481 gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt tcaataattt
     9541 gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca acacccgtgg
     9601 ctccatttat taatacactg agtccgttag gcggatattt gacagcggct atacattttt
     9661 gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct tcgtttattt
     9721 gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca ggctttcccg
     9781 gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg gcattctgac
     9841 gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata ttccctgtta
     9901 gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt tcctccagtt
     9961 taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta tatttgtatt
    10021 ccaattttta ctttgaaaca caaatagtcg aatatttgcc tcagatatct gacaaaataa
    10081 tttatctacc aatttagttt ttatctaata tgatttttat gtttttt
//
LOCUS       DAYYHB010000088         9826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000088 DAYYHB010000000
VERSION     DAYYHB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.090"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACHA6B_002218"
     CDS             complement(<1..205)
                     /locus_tag="ACHA6B_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949769.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACHA6B_002219"
     CDS             complement(221..835)
                     /locus_tag="ACHA6B_002219"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8949770.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACHA6B_002220"
     CDS             complement(982..1665)
                     /locus_tag="ACHA6B_002220"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8949771.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACHA6B_002221"
     CDS             complement(1774..2232)
                     /locus_tag="ACHA6B_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8949772.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACHA6B_002222"
     CDS             complement(2316..4079)
                     /locus_tag="ACHA6B_002222"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8949773.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACHA6B_002223"
     CDS             complement(4300..5760)
                     /locus_tag="ACHA6B_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949774.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACHA6B_002224"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACHA6B_002224"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8949775.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACHA6B_002225"
     CDS             complement(6917..8344)
                     /locus_tag="ACHA6B_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8949776.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACHA6B_002226"
     CDS             complement(8375..9589)
                     /locus_tag="ACHA6B_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8949777.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2914 a         2041 c         1628 g         3243 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaa
//
LOCUS       DAYYHB010000089         9810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000089 DAYYHB010000000
VERSION     DAYYHB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.091"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..2153)
                     /locus_tag="ACHA6B_002227"
     CDS             complement(33..2153)
                     /locus_tag="ACHA6B_002227"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8949778.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="ACHA6B_002228"
     CDS             2383..3561
                     /locus_tag="ACHA6B_002228"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8949779.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="ACHA6B_002229"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="ACHA6B_002229"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8949780.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="ACHA6B_002230"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="ACHA6B_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8949781.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="ACHA6B_002231"
     CDS             complement(4914..6167)
                     /locus_tag="ACHA6B_002231"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8949782.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="ACHA6B_002232"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="ACHA6B_002232"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8949783.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="ACHA6B_002233"
     CDS             7745..9091
                     /locus_tag="ACHA6B_002233"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8949784.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="ACHA6B_002234"
     CDS             complement(9399..9785)
                     /locus_tag="ACHA6B_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8949785.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1781 c         2010 g         2892 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtttccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DAYYHB010000090         9656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000090 DAYYHB010000000
VERSION     DAYYHB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.092"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..346
                     /locus_tag="ACHA6B_002235"
     CDS             197..346
                     /locus_tag="ACHA6B_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8949786.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            357..1871
                     /gene="dltA"
                     /locus_tag="ACHA6B_002236"
     CDS             357..1871
                     /gene="dltA"
                     /locus_tag="ACHA6B_002236"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8949787.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1868..3073
                     /gene="dltB"
                     /locus_tag="ACHA6B_002237"
     CDS             1868..3073
                     /gene="dltB"
                     /locus_tag="ACHA6B_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8949788.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3118..3351
                     /gene="dltC"
                     /locus_tag="ACHA6B_002238"
     CDS             3118..3351
                     /gene="dltC"
                     /locus_tag="ACHA6B_002238"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8949789.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3354..4622
                     /gene="dltD"
                     /locus_tag="ACHA6B_002239"
     CDS             3354..4622
                     /gene="dltD"
                     /locus_tag="ACHA6B_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8949790.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4763..5845
                     /locus_tag="ACHA6B_002240"
     CDS             4763..5845
                     /locus_tag="ACHA6B_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8949791.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5845..6648
                     /locus_tag="ACHA6B_002241"
     CDS             5845..6648
                     /locus_tag="ACHA6B_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8949792.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6718..7458
                     /locus_tag="ACHA6B_002242"
     CDS             6718..7458
                     /locus_tag="ACHA6B_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8949793.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7469..8473
                     /locus_tag="ACHA6B_002243"
     CDS             7469..8473
                     /locus_tag="ACHA6B_002243"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8949794.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8518..>9656)
                     /locus_tag="ACHA6B_002244"
     CDS             complement(8518..>9656)
                     /locus_tag="ACHA6B_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949795.1"
                     /translation="ILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGI
                     FCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVA
                     CTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDR
                     RQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEK
                     ILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQL
                     LIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELD
                     DQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3101 a         1593 c         1970 g         2992 t
ORIGIN      
        1 cacaacctct ttatttttta tattttttaa aagtacgaga aattataaag aaaaaagcag
       61 tgatttgatg tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt
      121 tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat
      181 atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa
      241 attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca
      301 ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg
      361 aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg
      421 cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg
      481 cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg
      541 agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga
      601 ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta
      661 tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa
      721 aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag
      781 gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac
      841 aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca
      901 agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta
      961 tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag
     1021 actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc
     1081 catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat
     1141 tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg
     1201 atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta
     1261 tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct
     1321 acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag
     1381 aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg
     1441 aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg
     1501 caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca
     1561 aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc
     1621 gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg
     1681 cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca
     1741 aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg
     1801 atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga
     1861 atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt
     1921 agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt
     1981 ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca
     2041 acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt
     2101 tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat
     2161 aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact
     2221 tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag
     2281 agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc
     2341 aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc
     2401 gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg
     2461 ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg
     2521 acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta
     2581 cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta
     2641 catgatggga tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa
     2701 agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat
     2761 gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa
     2821 tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta
     2881 catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt
     2941 taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt
     3001 tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact
     3061 tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg
     3121 gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat
     3181 cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta
     3241 gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat
     3301 tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa
     3361 agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt
     3421 ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt
     3481 ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac
     3541 aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg
     3601 ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac
     3661 aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag
     3721 tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta
     3781 atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc
     3841 ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata
     3901 cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga
     3961 atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg
     4021 ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc
     4081 aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc
     4141 cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca
     4201 aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg
     4261 tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg
     4321 agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga
     4381 tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg
     4441 gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag
     4501 atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg
     4561 acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat
     4621 aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa
     4681 gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga
     4741 atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac
     4801 ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc
     4861 caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg
     4921 tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt
     4981 aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca
     5041 agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt
     5101 gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat
     5161 aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat
     5221 acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata
     5281 tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga
     5341 gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga
     5401 agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc
     5461 tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat
     5521 tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt
     5581 attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat
     5641 ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt
     5701 agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga
     5761 ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt
     5821 tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa
     5881 aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct
     5941 ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa
     6001 agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac
     6061 tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt
     6121 ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat
     6181 tcagtcatca actggctact caacagttta atctttctgc ccatgttttt aatcagcgga
     6241 gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca
     6301 gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca
     6361 ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat
     6421 cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt
     6481 ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct
     6541 ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat
     6601 gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt
     6661 ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg
     6721 aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt
     6781 tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc
     6841 aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat
     6901 catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga
     6961 aatccggaga cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa
     7021 ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt
     7081 ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt
     7141 atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa
     7201 agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa
     7261 ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca
     7321 gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg
     7381 aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac
     7441 aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc
     7501 aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat
     7561 tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga
     7621 agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg
     7681 aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga
     7741 tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga
     7801 attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa
     7861 attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat
     7921 gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga
     7981 atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga
     8041 agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg
     8101 cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat
     8161 caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga
     8221 tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt
     8281 ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat
     8341 ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat
     8401 agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac
     8461 cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta
     8521 tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt
     8581 ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa
     8641 acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg
     8701 tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat
     8761 ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg
     8821 gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg
     8881 gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt
     8941 tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat
     9001 cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc
     9061 catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg
     9121 ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc
     9181 tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat
     9241 aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat
     9301 gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa
     9361 acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac
     9421 actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata
     9481 aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt
     9541 atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa
     9601 taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatat
//
LOCUS       DAYYHB010000091         9518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000091 DAYYHB010000000
VERSION     DAYYHB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.093"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHA6B_002245"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHA6B_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8949796.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1874..4540
                     /locus_tag="ACHA6B_002246"
     CDS             1874..4540
                     /locus_tag="ACHA6B_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8949797.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4904..5656
                     /locus_tag="ACHA6B_002247"
     CDS             4904..5656
                     /locus_tag="ACHA6B_002247"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8949798.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5678..6832)
                     /locus_tag="ACHA6B_002248"
     CDS             complement(5678..6832)
                     /locus_tag="ACHA6B_002248"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8949799.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7017..9458
                     /locus_tag="ACHA6B_002249"
     CDS             7017..9458
                     /locus_tag="ACHA6B_002249"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8949800.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3076 a         1715 c         2032 g         2695 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgcctt ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgta ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcgacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt tcagacagga ctagggccaa gatcaaaaaa
     1861 tgggggatga aatttggaaa acaaggaaca agcacaacaa gcggtctata aagaaacgat
     1921 cgactcgttg atgcagcaaa cgcagagctc aaccgatgga ttgagcacca cgcaagcgaa
     1981 ggaaaaatta gaaagagatg gcttgaacca gctaaaagaa aagccgaaga agccaacttg
     2041 gaaattgttc ttagaaactt tcaaagatgc aatggttatc gtattgctga ttgtcgcact
     2101 gattcagatg gtcatgggtg actacatcga attcttagtt atttttgcag tattgatgtt
     2161 gaattctata atcagtgttg tccaaacaaa aaaagcagaa ggttctttag aagcgttgaa
     2221 aaacctttca gcgcccgatg ctcgagtgat tcgtgatggt aaggaaaaaa cgattccggc
     2281 aaacgaactt gtggtaggag atatcgtgtt gttagacgca ggcgattatg tacctgctga
     2341 tggacgttta ctagatgcgg gatcattaaa agtagatgaa ggtatgctga caggagagtc
     2401 gacttctgca gaaaaagaag taacggatat cacaagtacg gtaccaatcg gagatcgtac
     2461 aaatatggta ttcagtggaa cgatcgtaac ttacggacgc ggtaaattct tagtgacttc
     2521 aactgggaat aatacggaga tcggacaagt tgctggcctt ttggaatcaa caactgagca
     2581 agtcactccg ttacaacgag gtctggataa attcagtaaa aaactaagtc tggctatttt
     2641 ggcattgtct ttattgattt taggtatcca actgtttcgt atttatcttg gagaaggtac
     2701 aggagatatg actgcagata tcgtcagcgc agtgatgttt gccgtagccg tagccgtagc
     2761 tgctattcca gaagcattac agtctatcgt aacaatcgtg ttatctttgg gaacgaataa
     2821 aatggcaaaa cgtcatgcca ttattcggaa gcttccagca gttgaaacac ttggttcgac
     2881 aagtatcatc tgtacagata agactggtac attgacacaa aacaaaatga cagttgttga
     2941 ctacttttta ggtaatggcg atactgggga tttcacaaat gacccttcaa aatggacagc
     3001 tgacgaacgt cgcttgatcg aaatcagtgt tttagcgaat gatgcatcga tcagtgaaga
     3061 cggtactaaa ttgggcgacc ctacggaagt agcgtttatc gactttagtg aaaaattaaa
     3121 ccagccttat caggaaatcc gaaacaacta cccaagacaa gcagaacttc ctttcgattc
     3181 tgatcgaaaa ttaatgtcta ctggacacac gatcgataac gaaaattact tgtttgtcaa
     3241 aggcggacca gatatcgtct tccaacgcag taaaaaagta ttgctaaatg gtgaagtcgt
     3301 tccgatgacg gatgaattga tgaaaaaatt ccaagagcaa aatgaagctt tttcaacacg
     3361 cgcattacgt gtactagctt ttgcttaccg tccacttgat aattttgatt tatcatttga
     3421 agatgaagac gatttgatcc ttgttggttt gcttgctatg atcgatcctc cacgtgctga
     3481 agtgactcag gcggtaacag aagcaagaag tgcaggaatc aaaacaatca tgattactgg
     3541 tgaccacaaa acaacagctg ttgctatcgc aaaacaaatc gggatttttg aagaaggaaa
     3601 cctggctttg accgggacgg aactagatga attgacagaa gatcaactaa acgaagtttt
     3661 agaaaaagtc actgtttatg cacgtgtttc acctgagaat aaaattcgta tcgttcgtgc
     3721 ttggcaaaat aaaggtcata tctcagctat gacaggtgat ggtgtaaatg acgcaccagc
     3781 attgaaacaa gcagacatcg gaattgctat gggaacgggt acagatgtgg caaaagatgc
     3841 agcagcgatg gtattgacgg atgataactt tgcttctatt gtcagcgcag tagaagtagg
     3901 acgtaatgtg tttgataata tcaagaaagc tatttcttat ctatttgcag gaaatttagg
     3961 tgcgatcatt gcgatcatct ttgccttagt tgctgactgg tccaatccat ttacagcatt
     4021 acaactgttg ttcatcaatt tagtcaatga ttcactgcca gcaattgcat taggtacaga
     4081 aaaagctgaa cctgctacga tgaaccgccc gccaagagat ccaaattcag gaatctttac
     4141 ggacaaaaca ttgatttctg taacgtatcg tggtgtgctg attgggattg ctgttatctt
     4201 tgctcaatgg cttggcatgc agcattcacc tgaattcggt gtagccatgt cattttcaac
     4261 attgatttgg tgtcggacat tgcaaacatt cccagcacgt tcaaacacac aaacagctat
     4321 ccaagcaggg ttcttctcta acagaagtgt cttactagct gtagttgtat gtagcgcgct
     4381 ttactgctta acaatgatcc caggactgaa agaagtcttc tctatcccat cagcatttgg
     4441 attgacagaa gtctttacat gtatgggaat cgcacttgtg acaattgttt tgatggaaat
     4501 cactaaattg atcattgtac gagtacaagg aaataaataa aacgaatcaa cgaggttgtg
     4561 agcctgccgt ttaattctga gtcacaaggt gcaatttcaa caaacagctt ctcatgtttg
     4621 ttgaatattg tgacttgtga ccgaagagtt ggcaggagaa caccgtttaa ggaagaagtt
     4681 gtaaaatcag cattttgagc taatactgag ggtcatctga ttggacactg tttattcgga
     4741 aagagggact ttgacacgac tttggtcaca gtcccttttt ttatttatga tagacggaaa
     4801 gccaatcatt ttattattca tcaaaagaga tagaatagat aaaagaggag aataatttat
     4861 tccgaaaagt gaaaacatat aacaaaaaat tagaggaaat caaatgaatg aaaaagaatg
     4921 ggatgaattt gctgaagaat attatgaaat ccagcaagaa tcgcaagtaa ctattgctga
     4981 agatgtaaaa aattatctga aaacggagaa agtcctgcct gctgaaacag cagcagacgt
     5041 tgctggagga tctggcagat ttctttcctt agcaaaagaa attaatcgct atgatttgat
     5101 cgatttttct tcaggaatgc tagaaaatgc aaaaaaagaa gcagaaaaaa atgagttgcc
     5161 aaatgtttcc tttatcaaac agtcgttaga agaatttatc caatcaaatg agacatatga
     5221 aatggttttt acagcagcaa atccagcatt ggtacatctt attcaattag aaaaattgat
     5281 gaaaaagtca agcaaatggt gcgtgataat cagggtagtg gaatcaatcg atgatgtttt
     5341 ttatcctatc gaaaagcaac taggaatgat agaagaagat cctaatacat cgccagttgt
     5401 aatggatcaa tttgaaacat acttaattga tgaaggattc aaagtgaaga aaaaagattt
     5461 tacttatttt acacaggaat caattactga agaattgatg aagctatact atgacgcgta
     5521 cgcaaaagat cctgttgtga ccaggtacat tcgcaaacta ttcagtgaaa aacgagaaat
     5581 ccttagtacg actaagctga cctatagact gctcctttgg tccgcagata aaaaaagaac
     5641 ctttcacgac tcgtgaaggg ttctttttat gattttatta tattttgtag taacgaatat
     5701 cattttcgat ctttgtaatc gaaaaaggta catctgaagt atattctgca cattctccgt
     5761 gttcaggaag cgtctcactg aaaatcgttt catattcgtc aacagtgatc cgtgttcgag
     5821 aatctagcat ctcttgatga gattgtgcga agagatattc ttgatagttt tcttccagat
     5881 aacctgtaaa aaattcactt actgcaccag aaccgtagct gaatagaccg atgcgatctc
     5941 ctggctgtag tgatttcgaa ttttccagta gagaagttag ccccaggtat aatgaaccag
     6001 tgtaaagatt accgattcgt cggctgtaac ggatgctttc ttcatagcga gccataagac
     6061 gttcctgatt ttcttcgtct gtttggtcta atacgctttg caatgccttt tttcccatct
     6121 tagtatacgg aatatggaaa gcaatcgcct gataatcttc gagtcctcga cccgacaatt
     6181 ctttatgtcg attccaaact ttttggaatg attcgatata cgtagaatta gataaaggac
     6241 catcaacaac aggaaattcg ctataatctg gacgccagaa atcatagata tcttctgtca
     6301 gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt ttgagtgatc atcatcgcaa
     6361 cagcaccgac accttgcgtc acttcaccac cgcttgccaa gccgtaacga gcaatatcac
     6421 ttgctatgac tagtactttt cgttctggat gatttttgac atattctttc gccatgtgca
     6481 aagctgcggt gccaccgtag caagcctctt ttacttcaaa agaacgagca aaagattgga
     6541 ttttcagtaa atggtggata attaccgcac tcgcttttga atgatcaatc ccggattctg
     6601 ttccgacgat gaccatgtcg attagctcac ggtctttttc tgttacgatt ttgctggcag
     6661 catttgctcc tagtgtaatg atgtcttcat ttgcacgatt gactgccatt tggtcttgac
     6721 caatccctat atggtatttt gcaggatcat ccccacggct ttctgccaac tctgtcatat
     6781 ctaaatataa attaggaata aaaaaggaaa gacgatcaat ccctattttc atttcaacac
     6841 ctctttactt atgataaaaa acttacttta ctaaggtatc ataaaaagat taaaagaaat
     6901 ataaaaaaga gaatatttaa ggattgttaa aatttaatct tagaaaaaaa caactggaac
     6961 aagagctatt tgtttttaca gcaaagaatg gtatattttt gtaggaggtg gcttacttga
     7021 aagaagtcgt tatgatagat gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa
     7081 gtccatttac tgcagttgaa ttaggcacac ttgtgactaa aggattatta gataaaacaa
     7141 aactgaaaaa agataaaatc gatcaagtaa tattcggaaa tgtcctgcaa gctggtaatg
     7201 gtcaaaatgt tgccagacag attgctttga acagcggtct ccctgtagat gtcccagcaa
     7261 tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt gatcttagca cgccagctga
     7321 tccaattagg tgaagcagaa cttgtgatag ccggcggaac agagagtatg tctcaagcac
     7381 ctatgctgaa accgtatcag tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg
     7441 tcaacgacgg attgactgac gcattttcaa atgcacatat gggattaacc gcagagaagg
     7501 ttgcaacaca attttctgtg agcagagaag aacaggatcg ctatgccttg tcgtcccagt
     7561 tgaaagcagc acatgctgtc gaagccggtg tattttctga ggagatcatc ccagtcaaga
     7621 tttctgatga agacgtgtta tctgaggatg aagcagttcg tggaaatagt acattggaaa
     7681 aactgggcac gttacgtaca gtattctcag aagaaggaac tgtaacagca ggaaatgctt
     7741 cgccattgaa tgacggtgcc tctgtggtga tccttgcatc caaagaatac gcagaaaata
     7801 ataatctgcc ttatttagca accatcaaag aagtagcgga ggtcggtatt gacccaagta
     7861 tcatgggaat cgctccaatc aaagccattc aaaaattgac agatagatca ggaatgaatt
     7921 tatcaacaat cgatcttttt gagatcaatg aagcatttgc agcttcttct atcgtagtta
     7981 gccaagaact gcagttagat gaagaaaagg tcaatatata tggaggagca atcgcattag
     8041 gccatccaat tggtgcaagc ggtgcacgta tcttgactac attagcttac ggattgcttc
     8101 gtgaacagaa gagatacggt attgcttcat tatgtattgg tggaggttta ggcttggcag
     8161 tcttgttaga agcgaatatg gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc
     8221 agcttacgcc atctgaacgg cgcagtcaat taatagaaaa aaatgtattg acacaagaaa
     8281 ctgcgttgat ttttcaggaa caaacacttt ctgaagagct ctccgatcac atgatcgaaa
     8341 accaagtgag tgaagtggaa atcccaatgg gcatcgcgca gaattttcaa atcaatggta
     8401 agaaaaaatg gattccgatg gcaacagagg aaccatctgt cattgcagct gcaagtaatg
     8461 gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca gcggctgatg cgagggcaaa
     8521 tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat catcgaaaag
     8581 aagagctgat tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg
     8641 ttcaagatat ctctactcgg gaattcatgg ggagtttcca tgcatactta tctattgatt
     8701 ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat caactcgatt cttgagagtg
     8761 tggcaaacaa gttacgggag tggttccctg aagaagagat tctattcagt attttaagta
     8821 attttgctac agaatcattg gcttctgcct gttgtgaaat cccattcgaa cgcttaggga
     8881 gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca acaggcaggg gaatatgcga
     8941 agcttgaccc ttaccgtgca gcaacgcaca acaaagggat catgaatggt atagaagctg
     9001 tagtggcagc tacaggaaat gatacacgtg cggttagcgc ttcgattcac gcctacgcgg
     9061 caagaaatgg attataccaa ggattaacag actggcagat caaaggggat aaactagttg
     9121 gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg agcttccaat atcctgccaa
     9181 aagcaaaagc atctttagct atgttagata ttgattcagc caaggaacta gcacaagtaa
     9241 ttgctgcagt agggttagct caaaacctgg ctgctttgcg tgcattagtt acagaaggaa
     9301 tccaaaaagg ccacatggga cttcaagctc gttcattagc gatctcaatt ggggctattg
     9361 gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga
     9421 cagctataca aatattggaa aaaatccggg aaaaataaaa aagcaatatc tttagaagaa
     9481 taaagcaaga tttacagtta ttttatttac tgcaaatc
//
LOCUS       DAYYHB010000092         9322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000092 DAYYHB010000000
VERSION     DAYYHB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.094"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHA6B_002250"
     CDS             <1..107
                     /locus_tag="ACHA6B_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8949801.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHA6B_002251"
     CDS             complement(176..904)
                     /locus_tag="ACHA6B_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8949802.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHA6B_002252"
     CDS             complement(917..2197)
                     /locus_tag="ACHA6B_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8949803.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHA6B_002253"
     CDS             complement(2338..2940)
                     /locus_tag="ACHA6B_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8949804.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHA6B_002254"
     CDS             3185..3391
                     /locus_tag="ACHA6B_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949805.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHA6B_002255"
     CDS             3703..4422
                     /locus_tag="ACHA6B_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8949806.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHA6B_002256"
     CDS             complement(4503..5186)
                     /locus_tag="ACHA6B_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8949807.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHA6B_002257"
     CDS             5405..7591
                     /locus_tag="ACHA6B_002257"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8949808.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7937..8347
                     /locus_tag="ACHA6B_002258"
     CDS             7937..8347
                     /locus_tag="ACHA6B_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8949809.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8361..8717
                     /locus_tag="ACHA6B_002259"
     CDS             8361..8717
                     /locus_tag="ACHA6B_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8949810.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3364 a         1525 c         1745 g         2688 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
     7861 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
     7921 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
     7981 agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
     8041 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
     8101 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
     8161 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
     8221 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
     8281 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
     8341 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
     8401 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
     8461 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
     8521 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
     8581 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
     8641 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
     8701 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
     8761 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
     8821 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     8881 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     8941 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     9001 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     9061 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     9121 tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa aagagttgac aattacttgt
     9181 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     9241 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     9301 attcaattca agacaacaaa ca
//
LOCUS       DAYYHB010000093         9146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000093 DAYYHB010000000
VERSION     DAYYHB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.095"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            547..1227
                     /locus_tag="ACHA6B_002260"
     CDS             547..1227
                     /locus_tag="ACHA6B_002260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8949811.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1256..2569
                     /locus_tag="ACHA6B_002261"
     CDS             1256..2569
                     /locus_tag="ACHA6B_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFM8949812.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3063..3374
                     /locus_tag="ACHA6B_002262"
     CDS             3063..3374
                     /locus_tag="ACHA6B_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFM8949813.1"
                     /translation="MNMEATLKLEKRNKLIHFYPGGSREMLCRCKYCEPERIQVKTGF
                     SFSVKEESFQVLLCISGDGQVESANGVMKLIRFVKKILYVFANEYWKMLSVERCGLTE
                     N"
     gene            3399..4142
                     /locus_tag="ACHA6B_002263"
     CDS             3399..4142
                     /locus_tag="ACHA6B_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFM8949814.1"
                     /translation="MSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVVTPNVDHIV
                     QLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLCKMAAEKGY
                     TMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDSQIKRANPD
                     MLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSDYGFEWLFR
                     ITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4169..5170
                     /locus_tag="ACHA6B_002264"
     CDS             4169..5170
                     /locus_tag="ACHA6B_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFM8949815.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5193..6479
                     /locus_tag="ACHA6B_002265"
     CDS             5193..6479
                     /locus_tag="ACHA6B_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HFM8949816.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            6513..7166
                     /locus_tag="ACHA6B_002266"
     CDS             6513..7166
                     /locus_tag="ACHA6B_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8949817.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7199..8404
                     /locus_tag="ACHA6B_002267"
     CDS             7199..8404
                     /locus_tag="ACHA6B_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8949818.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            8480..>9146
                     /locus_tag="ACHA6B_002268"
     CDS             8480..>9146
                     /locus_tag="ACHA6B_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949819.1"
                     /translation="MEIRISYYFRKLLLFYVCVIVAAMSYHLTGNSNVASLIAVVGVV
                     YAIVKACNNEYLAIWVMFVLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKV
                     KARSRYGIVYSVMLIFILIACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSE
                     RITKNEFIDLVCKFTRWTMCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILY
                     FFIN"
BASE COUNT         2917 a         1325 c         2175 g         2729 t
ORIGIN      
        1 tatcgtagga tgtgatgatg atagccgtga agttgttcaa gtatttacca atgtctacaa
       61 cgcagaaaat tagtttgggt ttttcggatt ggaaaatttc aatgtataaa atcataaatt
      121 tgctgtaaat agacagtttg gggtagtaat agcccaataa attgttcgat aaactcatct
      181 ttggggtgag aatagctcaa tcattgaatt tcaaacctgc ctttggggtg ggattagagg
      241 tcaatatgta cctacataat actttttgga aaatgatggt gttgagaatg taagtacatt
      301 tcaatgtgaa attctaatat agccccaaac gaggtcaata aaaatgacct aaactcgtat
      361 ttctccccta aacgaggagg agatagatat gaaaattgaa tgtgccggcg ttggagttgg
      421 ctgcttttag taggaatgaa catggttcga tgagaactgt ttcagataga gtggtcgatg
      481 atcacaggcc tttaagtcgt ttacagatga tgagacttgt aaaaatagag ggacgaatgg
      541 aaaaagatga ttggtataga agaaaagaaa ggtgttcaga aagacgtaac aattgatagg
      601 gaagagaatc ttaggggaca tagattctat tggattggac gcagagcaca agatatcttc
      661 ttctcggctc tagctttgat tgcattgagc cctgtgatgc taattacagc gctcgccatt
      721 gtgattgacg atcccggtgg cagcccgatt tttgtgcagg atagagttgg aagaaatgga
      781 aagctgttca agttttataa attccgatct atgtgtgtag atgctgaagc aaaattagaa
      841 gccttgttgg cacaaaacga gatggatggt ccggtcttca agattaaaga tgacccaaga
      901 atcacgagag ttggtaaatt tattcgaaag acaagtatcg atgagttacc tcagctctgg
      961 aacattctta aaggtgatat gagtattatt ggacccagac ctgctttacc gagagaggtg
     1021 gaacaatacg gtgactacga aaggcagcgg ttgttcgtac agcctggtct tagttgctat
     1081 tggcagattg ctccacatcg taatgaactg tcctttgaag aatgggtgga acttgatgtg
     1141 aagtacatca atgaaagaag ctttatcacg gactggaaga ttatcttctt gactttacag
     1201 gctgtattat ttggatatgg tgaatgatta tatatagtat taggagttga aatatgtgaa
     1261 tattatttta ttatcgggtg gtagcggtag gcggttatgg ccgctgtcca acgatattcg
     1321 tagcaaacaa ttcatcaaac tgtttaagga tgaaaatggc gaatatgaat ctatgattca
     1381 aagagtttat cgccagatta caactgttga cccagatgca aaggtcacta ttgcaacaag
     1441 taagtctcag gctagtgcaa tccataatca gttgggagat aaggcttcga tttgtgtaga
     1501 accttgtcgt agagatactt tccctgcaat tttgttagct gcagcatatc ttcacgatga
     1561 acttggcgta ggtgatgatg aaagtgttgc ggtttgtcct gttgatcctt atgtagataa
     1621 ttcctattac gaaggcgttg aagcccttga gaagttggtg cagcagaata cggctaattt
     1681 gactttgatg ggtattgcac ctacatatcc tagtgaaaag tacggttata tcatccctga
     1741 aagttctaac gaagtaagta ctgttaagga gtttaaggaa aaacctgaca aagcaacggc
     1801 agagaaatat ttagctcaga atgctgtttg gaatgccggt gtatttgcgt tcaaacttgg
     1861 ttacctgttg gaaaaagctc acgcaatggt ggattttgaa gattatcgtg atttgtattc
     1921 caagtatgag actttaacca agatcagctt tgactatgct gtggtcgaaa aagaaacgag
     1981 cattcaagtg atgcgttata acggggattg gaaagatgtc ggaacttgga acatgatggc
     2041 tgaagttatg gctgataata ttaaggggaa agctgtaatg gatgatacct gtgttaatac
     2101 tcagattgtg aatgagttga atattcctat tcttgctatg ggctgcaaag atatggtaat
     2161 tgcagcaagt ggcgatggaa ttctgatttc tgataaagag cgcagtggct atatgaagcc
     2221 gtatgttgag aaaatcagta cggaagtaat gtttgcagaa aaatcctggg gtacatatac
     2281 cgttatggac gttcaggaag gctccatgac tgttaaagtt tctatgcagg ccggacagaa
     2341 gttgagctac cactaccaca attctcgcga tgaagtttgg actgttgtag caggacaggg
     2401 taaagcgatt gtagatggta tgaagcagat tcttcgtgcg ggtgatgtgg tgactattgc
     2461 cgcaggatgt aaacatacaa tcgaagccgt gacaaatctg gatattatcg aagttcagat
     2521 tggtgaaact attagcgtcg ctgacaagta taagagttct ttgaactaaa agttcgcgag
     2581 gtagttatta tggagatact aaaatttcaa cccgtgggta aagattatct ttggggtgga
     2641 gaacggttta agacggaatt tggaaaaaat tgacttaaca ccattaagct tatacgtggg
     2701 aatgcttagt tcatctagac gataactacg tacggaaata tcaaatatga aggacagaat
     2761 ggtaaaaccg agttgtggta tgtattggaa gctacaccgg ggggtgattt atgcttttga
     2821 gtacgatgta acagaagaaa tgttacgaaa tgctgtgatg gacagatcac ttgggaaaca
     2881 tttatataaa gaagaagtac acaagggaga tcttttttat gtcagttcat ggtattggcg
     2941 ctggagtatt agtggctgaa atacaggaaa gctcaaatgt tacctatcga gtttacgatt
     3001 atgatcgagt agataagaat gataataagt gagaattgta ctttgaaaaa gcgattcagg
     3061 taatgaatat ggaagccact ttgaaattgg agaaaagaaa taagctcatt catttttatc
     3121 ctggtggctc tcgtgaaatg ctgtgtagat gtaaatactg tgagcccgaa agaatccaag
     3181 taaagacggg gttttcgttc tcggtaaaag aggaatcttt tcaagttctt ttatgtatat
     3241 cgggggacgg acaagttgag tcggcaaatg gtgttatgaa actcattaga tttgtaaaaa
     3301 agatattgta tgtttttgcc aacgaatatt ggaagatgtt aagtgttgag cgatgcggac
     3361 ttactgaaaa ttagatgggg atagagggag gatatctaat gtcacgaatg agatttatga
     3421 acacagaaat tgacaatctt acaatgggtg aagtattggt agaggttgaa aaaatgattc
     3481 aagataggag atgcggatat gttgtaactc cgaatgtgga tcacattgtt cagttggaaa
     3541 caggaaaaga gttgcaagat gtttataaaa acgcatcttt aattttgacg gatggtaaac
     3601 cattgatttg gatttcaaag ttttataaga caccaataaa agaaaaaata tcgggatccg
     3661 atatattttc tttactgtgc aagatggctg cagaaaaggg atacacaatg tattttttag
     3721 gtgcggcaga gggcgttgcg gctaaagcgg cagataacct gatgaaaaga tttccgggat
     3781 taaatgttgt aggaacatac tctcctcctt atgggttcga aaaagacgaa gatgaaatgt
     3841 ttaaaattga ttcccaaatt aaacgagcaa atccagatat gcttattgtg ggtttgggtt
     3901 gccctaaaca ggaaaaattt atgttttata actgtgatag attgggagta cctgtgtctt
     3961 ttggtctagg agctagtttg gactttgagg cagggaatgt taaaagagca ccaaaatgga
     4021 tgtcagatta tggatttgag tggctgttta gaattactca ggaccctaaa agaatggcaa
     4081 aacgatattt ggttgatgat ttaaaaatct ttaaattagc actaaaatat cggaaaaaat
     4141 aaactatatt caatgaggac aatgaagtat gattattaca aaaacacctt ttcgcatgtc
     4201 tttttttggc ggcggaacgg atatggaaga ttattttcga gaaaatggcg gtgcagtgct
     4261 ttccactact tttgataagt actgttatgt gaatgtgcgt catttgccta gattttttga
     4321 ttacagcaca gagttatcat attctcgtac tgagagagta acaaatattg aagatatcga
     4381 gcatcctgca attcgtaatg cgatgaaaat gctagatatg catgaaattc gccttacata
     4441 tgaagcagat cttcctgcac gttctggtct gggtacatct agttcttttg ccgtaggtat
     4501 gttgaatgct ttctatgctt tgaagggcaa gtacgctgac aagaagaaac tcgttgataa
     4561 agcaatttat cttgaaagag tcctttgtaa tgaagcaggc ggatggcaag atcagattgc
     4621 agcagcttat ggtggcttta accgaatcaa tttcaactca gatggctatg aagtattgcc
     4681 tattattatt acgcctgagc gtaaaaagca gcttaacaac aacttgatga tgttctttac
     4741 tggattcacc agattttcct ctgatgttca gaaggcaaat aatgtcagcg gcacagagga
     4801 caaaagagta agactgaaaa aaatgtatga gcttgtggat gaagcagaag cggttctgac
     4861 tgataagaac agagatctag atgatttcgg cagactgctt gatctcactt ggagattgaa
     4921 aaaggctacc ggtggtgcga ttagcactgg aagtatcgat gaactttatg aaaagggcat
     4981 ggctgctggt gcattgggcg gtaaacttct cggagcaggt ggcggcggtt tcttggtttt
     5041 ttatgttcag cctgaaaaac aaaatgctgt gcgttcggca atgagtaatt tgatgtatat
     5101 tccgtttgaa tttgaaaacg gtggaacaca ggttattcat tacacaccgg aaatgtatat
     5161 tccaaaataa agaaatcagc gaggataaga atatgaaagt tgttattatg gcaggcggta
     5221 agggaactcg catctcatcg gttgcaagcg atattccaaa gcccatgatt aaaattgaaa
     5281 acaaaccggt attggagcac gaaatcgaat gtctgagaga ccagggattc acagatatta
     5341 ttcttaccgt tagccatctt ggcgatatca ttatgaacta ttttggcgat ggttctggaa
     5401 tttcaccagt tactggtcag ccctttgacg ttaaaattga atactttttg gagaaagagc
     5461 cattaggaaa tgccggtgca ttgtttcgtt taaaggataa tttgaccgaa gacttcctcc
     5521 tgctgaatgc agatgcgatg tttgacgttg atttcaaccg atttgtagca tatcaccgtg
     5581 aaaagggtgg cttggtgaca ttgtttactc accctaacag tcatccatat gacagcggtc
     5641 taatcattgc agataaggat ggctctgtag acagatggct tgtaaaggaa gattcccgtc
     5701 caacttatta ccgtaacaga gtaaatgcgg gactccatgt gattagtcct gctgttcttg
     5761 atgtcgagat aaacacacct aagattgact tggaccgtca gttattaaag ccgttggcag
     5821 gaaccggaaa aatgttctgc tatgatagtc cagaatacgt aaaagacatg ggtacccctg
     5881 atcgttatta tgctgtctgt gaagatttta aggctggaag agtgaagggc aaaaatcttc
     5941 agaataagca gaaagctgtt ttccttgatc gtgatggaac gattaatcaa tatgttggct
     6001 ttctgcgtca tattgatgag ttcgaactaa tcaagggtac tgaagatgca gtgaaaaaga
     6061 tcaatgccgc tggatatctg gctattgtaa taacgaatca gccagtaatt gcaagaggtg
     6121 aagtttcttt tgaagaattg gaagagatcc ataacaaaat ggagacactt cttggatata
     6181 agggcgctta tttggatgca gtttattatt gcccgcatca tccacataag ggatatgagg
     6241 aagagcgccc agagctaaaa ttcgattgtg aatgcagaaa gccaaagccc ggaatgttaa
     6301 ttcaggcagc tacggacttc aacatagatt tgagtcagtc ttggatgatt ggcgatggcg
     6361 agaatgatat taaagcgggc caagccgccg gctgtaagac tgcattggtt ggcgagagtg
     6421 agtatggaca ggatatgacc atagcaaccc tgagtgaatt tgcagataag atctgctaag
     6481 ggatgtttta tcacgaaagg agagtataaa caatgataga caatagatta cagaaacaca
     6541 ttgatttgct gattaaacgc tatcccactc ttgaagtaat caagcaggat attgttgacg
     6601 gatacctgat tatggaggaa tgctatttaa atgatggcaa gctgctgata gcaggaaatg
     6661 gcggatctgc tgctgattcc gagcatattg cgggtgagct gatgaaaagc tttaagacgc
     6721 ctcgccctgt tagtagggaa tttgcagata aattgattgc ggttgatcct gtaaagggtt
     6781 ctgaattagc taagaatctt gaatatggtt tgatggcgat tcctctcgtt gctcatgaag
     6841 cgctttccac tgcgtatatt aatgatgttg atggcctagg tgttttcgca cagcagttgt
     6901 ttggatttgg aagacctggc gatgtattcc ttggtatttc gacatccggc aatagcaaaa
     6961 atgtgataaa tgcaactgtt gttgctagag ctcttggtat gaaagtaatt ggcttaactg
     7021 gagccaaagg tggagaactt gcaagggtag cagatgtttg tgttaaagct cctgaaacag
     7081 aaacatatat gattcaggag taccatttgc caatttacca ctgttggtgt ctaatgttgg
     7141 aagataaatt ttttgggaat agataaaata tctaagccca tattgaaagg ataaaattat
     7201 gaatgtacta tttataacaa ctcaatttcc atatcctcta gataatggcg gaaaaattgg
     7261 tgcgtacaac ggtatcagtg ttttatctag agactacgat gtgacggtat tgtctttcac
     7321 cgaagaaccg cagtatgttg aagaaggtgt cagttttttt cgagagaaat ttaatagtgt
     7381 aaaatttgaa aatcctgtga tacatgatgt acacataaga aataatccaa tgaaattggt
     7441 agaagcttta gcaatagggt attgtctacg ccaaccttat atagtttcaa agttttatga
     7501 ttcaaaaatg ctgcagttaa tagatgaaaa attttctaac aatgcatatt gggatatcat
     7561 ttttattgac tatctgaata tgcaaagtta tggtcaatac atacgggaga agtataaaac
     7621 acaatttggt aagtatatct taaaagacca caacatagaa tatgatttag tcctacagga
     7681 agctgaatca agtcgaggtt taaaaaagaa gttgcttcag ttagagtata agcgtactcg
     7741 aaagtatgaa gaggccgcag taaatagggc cgattattgc tttagtgtgt gtgatgcaaa
     7801 tacggaagta ctgaaaaaga ttaatgacaa cagttatacg atgcttccaa cttatgaaat
     7861 gcttacaggg gtgaaaaaaa atatagagaa gaacacgatc ctatatttgg gtaacctttc
     7921 ttggaaatcc aatatgcaag gattagtttg gtttttagag tcaatttatc caaatattca
     7981 acaaagaatt ccggatgtaa aaatcacaat tgtcggtagc gggccagttg gaacagattt
     8041 tagtaaatat tcaggcgtgg attatcgtgg gtatgtaaaa gatatttcac acatttatga
     8101 cgatcaagcg gtgctaattg tacctttgtt agaaggtagt ggtataagaa taaaaatatt
     8161 agaagcgttc aataatgaaa ttgcagttgt atctacatta gttgggtgtg atacgattgg
     8221 cgcaaaagat ggaagtgagt tatttatagc agataacgaa aaagattttg caacggctgt
     8281 agttagactg cttgaagaca tgaatttgaa tgaaaaagta agaaagaatg caaaattatt
     8341 cttaaaagaa aaatactcct taacggctag acaagatgag tttagtgcga taattaacag
     8401 ctaatatata tataagctta tctgatgatg atttatagga ggaaatatat ggagatggct
     8461 gagagaaagg aaaagaaact tggaaataag aatatcatat tattttagaa aactcctgtt
     8521 attttatgtt tgtgtgattg ttgcggctat gagttaccac cttacaggca attccaatgt
     8581 agcgtcatta attgctgtag taggagtagt ttatgctatt gtaaaggcgt gtaataacga
     8641 gtatctggct atatgggtta tgtttgtatt aggttctgat atatggggga tagtgccata
     8701 tattaacatt ggtagtggtg tgttgcattg gtcagatttt aatgttatag tagcgatatt
     8761 tctctgcttg agattcatca aagttaaagc taggagtaga tacgggattg tctatagtgt
     8821 aatgcttata tttattttga ttgcatgtgt gcaatcgcat tcaatctatg gacaagatgt
     8881 acatacaacg ctcttaacag ctagagaatt gctagttgta atatcctttt ggccgatagt
     8941 aaaaatagta tattcagaaa gaataactaa aaatgaattc attgatttag tgtgtaaatt
     9001 cacaagatgg acaatgtgta tatattggtt tgaatggatt cttgttaata tgggattcaa
     9061 tataaccttt ttacagacag gtgcacgctg gggtactaga atctatatag atccgatatt
     9121 tatcatttta tatttcttta taaatc
//
LOCUS       DAYYHB010000094         9073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000094 DAYYHB010000000
VERSION     DAYYHB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.096"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..121
                     /locus_tag="ACHA6B_002269"
     CDS             <1..121
                     /locus_tag="ACHA6B_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8949820.1"
                     /translation="FIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            146..391
                     /locus_tag="ACHA6B_002270"
     CDS             146..391
                     /locus_tag="ACHA6B_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949821.1"
                     /translation="MTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIVSYSI
                     LTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            598..798
                     /locus_tag="ACHA6B_002271"
                     /pseudo
     CDS             598..798
                     /locus_tag="ACHA6B_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1180..1326
                     /locus_tag="ACHA6B_002272"
     CDS             1180..1326
                     /locus_tag="ACHA6B_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949822.1"
                     /translation="MELYFSGISQRTISLTVGSSRHTIREVTSRADRKGVTELTADMS
                     DTWL"
     gene            complement(1510..2115)
                     /locus_tag="ACHA6B_002273"
     CDS             complement(1510..2115)
                     /locus_tag="ACHA6B_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8949823.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2297..2632
                     /locus_tag="ACHA6B_002274"
                     /pseudo
     CDS             2297..2632
                     /locus_tag="ACHA6B_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2797..3012
                     /locus_tag="ACHA6B_002275"
                     /pseudo
     CDS             2797..3012
                     /locus_tag="ACHA6B_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3150..4121
                     /locus_tag="ACHA6B_002276"
     CDS             3150..4121
                     /locus_tag="ACHA6B_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8949824.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4261..5574
                     /locus_tag="ACHA6B_002277"
     CDS             4261..5574
                     /locus_tag="ACHA6B_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFM8949825.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5617..6483
                     /locus_tag="ACHA6B_002278"
     CDS             5617..6483
                     /locus_tag="ACHA6B_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949826.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6480..7328
                     /locus_tag="ACHA6B_002279"
     CDS             6480..7328
                     /locus_tag="ACHA6B_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8949827.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7342..8814
                     /locus_tag="ACHA6B_002280"
     CDS             7342..8814
                     /locus_tag="ACHA6B_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8949828.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8831..>9073
                     /locus_tag="ACHA6B_002281"
     CDS             8831..>9073
                     /locus_tag="ACHA6B_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8949829.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2874 a         1535 c         1850 g         2814 t
ORIGIN      
        1 ttttattcaa tctgtcgcat ctaaacgaaa taatattcct agaaaatcac tggattatcg
       61 aacacctttg gaagtatttt tgagttacgt aagtattgat gatctgtcta acttaatttg
      121 acaattaaga catttaaaaa gaaaagtgac agataggaat aaaaacttat taaatgtagt
      181 tatgatctgt tatatttcat ttctaatact aggtcttcca caagcagtgt ttcctgaaat
      241 gttttctgga gactggggta ttgtcagcta ttctatatta acattgattg ctatggtttt
      301 aggtacttca ttttataatg cttttgtaag taagcatgtt gaggaatatg aagtattaat
      361 gtacttaaaa gagaaaaaaa caagtaatta atatgagaac accacgcata gtttttattt
      421 tgagatattc tagaatgaaa cacattaaga acgaaagcaa agcaaaagac gccagaactt
      481 ctactacctt ctaagttttt tcatatttgt ttgttatgct atacatagat ctgcataatt
      541 agacttgcgc tgcccgttat tgaagtttca ggcagcgctc tttttggggg atcgataatg
      601 gaagaagaaa aaatatttga gaaacgctgg cagctagcat caagtgaaca aagagcaaga
      661 tacaataatt tgatcacctc ctaccccact gttgactaga catataagga aaagaaatac
      721 ttactttggc tgtgccagtt agatatagat acctttgaaa catttgaagt gatcctagat
      781 aaaattaaac agaattgatc agaaacaagc gaagccttct ctcggttcgc ttgttttgct
      841 atttattagt tggctcaggg caactctaag ctcgtttgaa agaaaaccga tggataatcc
      901 ccttgtttgt accactttag aatacatgga tgtacgtatt gccattgact cctattagtt
      961 actgaaaaaa aatttaaaca agttgagtat tgcagataaa tagctccagt gaggtgaata
     1021 actgctccac tcaaggcaat agatagctcc atccagggca acaaactgct ccactttcat
     1081 atttaataga tgaataacct tctgtataat agaaaggcat acctttcggt atgacaactt
     1141 atacagaatg ttattttatt ggttcactat cgaaaaattt tggagcttta tttttccggt
     1201 atttctcaac gaacgattag tttaactgtg ggaagctcca gacatacgat tcgagaagtt
     1261 acttctcgag cagacaggaa gggggtgact gaactaaccg ctgatatgag cgatacttgg
     1321 ctgtaggaat atctttttcc ggaaaaacaa acaacagcac gaggttacct tcctgtagac
     1381 tgggaatatg ttcataaaga actaatgaaa aagaatatga cattgaaact ccttcataga
     1441 gaatatgggg tacatggaac atagaaacga aaatgcacgg tagaaaagcg aatgaatata
     1501 gtataaattc tacaaatcat tcgctttctt tattttatat atggttggtc gagagagacc
     1561 attttctttg gcaatttctg caactggaac tccttgattt aacatgtcca ccacagtttt
     1621 aaagactaat cgtttttgcg gatctgttga gttctcagaa tattcaattg gtcgcccctt
     1681 atatcgacca gcttttttgg ccaataatac tccttgccgc tgacgttcac ggattttctc
     1741 acgttcgttt tgtgcaatat aacttagcaa tgataaaaac atatcgaaca tagcagtatc
     1801 taggagttca tttccagtat taaaatctaa aaactgtgca tctagtattt ttaatgctac
     1861 ttttttctgt ttcataaagg taactgtatt ttttatgtct tcataatctc tgcctaggcg
     1921 atccagtgat gcaacaatga cacaatcacc ttctcggata taatttagta atttttgaaa
     1981 ctcaggtcgc tggatatctt taccactggc tttttcttgg aataatttat caatgttaag
     2041 gtcttccatc attgccaatt gtcgatctaa attttgatct tgtgaagaca ctctaatata
     2101 tccaattttt gccataaaaa ctccttttaa aaaatgatat aatttacatg taaaatttac
     2161 ttgtagtttt actgttatca tagcattttt aaaaataaat tttaagtcta cttataattt
     2221 actttggagg ttttgaatga atgaaattaa gcgcaataaa aattcttttg gtgtttattt
     2281 aggagagcct agctcggaac agttaaataa aggtgaagct cgtcatgctc tatgtcgaaa
     2341 tatattttat ggtagaaaag gcaaacttta tcaaacttat tacgacggta tggaggaaca
     2401 gctcaacgca ttaggattag taactaatgc tatcatatat tggaattcac tatatttaga
     2461 aaaagtcttt gaacaaatta aaacggaagg gttcgactgt tccgaagaag tgttacgcaa
     2521 gcaatttcca ttaattagtg atcacattaa ttttgttggc aaatatagtt tccgctatga
     2581 aaaagaatta tcgaatggaa tactacgtcc gttaaatata gagaattatt gagaatgttc
     2641 gtcttttggt tcctaccgtg cattttcgca cggatgttcc agatactatt tttgaatgta
     2701 taaaattgaa aacacaagaa tactaataaa tcaacgattc ttgtgttttt aatttttcac
     2761 taccgtgcat tttcgtttct atgttccatg taccccaatc ttgaaaaatt aattaaagac
     2821 ttagataaag ccattgacga cttagtcgtg gtgctaccgg agtatcaatg tttaaccagt
     2881 atcccaggta ttggtcctgt ttaggctgct ggaatactcg ctgaaattgg tccaattgaa
     2941 cgtttttctg atgaaataaa attgacaaag tatgccggcc tatattggcg acagagtcaa
     3001 tccggtaaat ctgaatacga aaattttttt acgaaaagct tcacgtctaa tatctatttt
     3061 aaaataaaaa tattcctgct aatttggaat cgtttccttc ttttggtata ataatacctg
     3121 aaattctttt gagaggagtt gttcgtgaag tgaaaactac tatgaaggat gttgctgcgt
     3181 tagcaggagt tggtgtgggt acggtatcta gagtaataaa tggagtaaag gtgaaagagg
     3241 ttaccaaaga aaaagtaatg gctgcaatta gtgagcttaa ttatgagcct gatgaatatg
     3301 ccagaggtat gaaaacgaat agtagcaata caatagctct tattttacct acgatttggc
     3361 atcccttctt ttccgagttt gcctactatg tagaggaagc tctcagccaa aaaaattata
     3421 agcttttttt gtgtaatgcg aacggcagtg cggaaaaaga aaaagaatat atccaaatgg
     3481 tcaagcagag taaagtagac ggaattatag gtattaccta ttcagacatt gataaatatg
     3541 tatcatcaaa tttaccattt gtcagtatag accgccattt ttcggaacag gtttcttatg
     3601 taacggcaga taattattta ggtgggcaaa ttgccgctca agaactactt actcgtaact
     3661 gtaaaaactt gtgctatgtt gggggtgttt caccttaccc taacgaaaca aacaatcgta
     3721 aagctggttt ttacaatatt tgtgaggaaa ataaaatagc tgtgaaggta ttggatatgc
     3781 ctgaaccaat tagcaattta gaggaacaat tgctcgcttt tttttcggat aatcctgaaa
     3841 ttgatagcat ttttaccatc aatgatttca tggctttgga tgtgataaaa gcccaaaaaa
     3901 aactagggaa ggaagcacct aaggattttc aaattattgg ttttgatgga gtcaagctat
     3961 cgttgcaaaa agactattta ctatcaacga ttgtacagcc gatagaggaa atggccaagg
     4021 tttcagtaga aacactcatg gcgatgattt taaatgaaga accgcctaag cgtgtggtag
     4081 taccggtttt ctttcacgct ggagagacga ctaaagagta aaaaaccggt ttataacgat
     4141 ttttttgaga taggttgaca gaaagtgatt tcaatgatat cttaaaacca attaattgga
     4201 aacgttacca attaattgaa aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg
     4261 atgaaaaaga gaatgttagc aattgcagca attttagcat taacagctgg aggacttgca
     4321 ggatgtggca gtaaggagaa aagttcaggt agcagtgatc caggagaaat taagatttgg
     4381 gttcagtttt ctgatgaaac gcctgaagga aaagcttggg cagagattgt gaacaatttt
     4441 aatgatgaat acgagggtga atataaagct gttactgagt atattcctcg tagcggaagc
     4501 ggcggtggat atgaggataa agtaaatgct gcaattacaa ccaataccct tcctgatgtt
     4561 attacgttag atggaccgaa tactgcagct tatgctcatt caggagtcat tgctccatta
     4621 gatgattatt tgaaagatgc ggacatggat gatgtattag acagcattat tcagcaagga
     4681 acctacgatg gcaaaatgta tgcgtttggt ttctcagagt ccagtgtggg tgtctattac
     4741 aataagtcgc tgttcaaaga tgctggaatt acagaagatc aattgccgac gttagaagat
     4801 ccttggactt ggacggaatt tcaagcaatt tgccaaaagc tgactgataa atacaaaacg
     4861 cctgcaattg atatgcaaat ggcgtcggga gacgaaatgc tgacctacgc atatacacca
     4921 tttatatggt cgaatggcgg cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat
     4981 tttaacggta aaaaatccgt tgaagcgttg gaatttattc aagaattggt tgaaaaaggt
     5041 tacaccacaa tcagtcctat tgaaaaagga tttgaaactg gcaaatatgc catgatgctg
     5101 agcggttctt ggacaatggc agatttagag gaaaactatc aagagattga ttatggtatt
     5161 ttgccttacc ccgtttcaga tgcaactggg gagttagttt caccaaccgg aagctggcag
     5221 ttagcaatga ctgagaaaac agagaaaaag gatgcttctg cggcctttat taagtttgca
     5281 accagtacag aatctagtcg tattatgagt ttagggaaca gtgttctacc tatcagaaat
     5341 tcaacagttg atttaattaa agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa
     5401 aaccaacttt ctggacatgc ccgcccagtg gttgtagctt atccgcaagt atctagagca
     5461 ttccaacaag cagttcaaga tattagctac ttcgaggaaa acccagatgt acaaaaagta
     5521 gcagatagtc gcgctgctga aatgcaacag gcaattgata cttcactaaa atagaagttt
     5581 ctaaaggggg ccaatcaagt gttaaaaaaa ccgacaatga aagacaattt gataggttac
     5641 ggctttctag cgccagcttt gctgctgctg acgatttttc tgatcatacc cgtaatcatg
     5701 gtaatttatt atgcctttac tgattattat ttgttaacgc cagatgctag acaatttatc
     5761 ggcttagaaa attttaagcg actgttcgct gatccaattt ttgtaaaaag tatttggaac
     5821 acggctaaat ttgttgtttg gattattccg gtccaaatag gggcagcttt aggaatggca
     5881 cttattatca acaaaaatcg taaaggaaat atctttttta aagtagcctt ttttgcgcca
     5941 gtcgtgatgt ccttagtcgt tatttcgatt ttatggttgt acttgttaaa tcctaatgaa
     6001 ggtctggtta atacgctatt tgcaaagatt ggaattgctt cgcagccttt tttgactagt
     6061 cccaaacaag caatgtatac gcttgttttc gtatcggcat ggcaaggtgc aggatatcag
     6121 atgctgctgt ttttaggggg aatgcaaaat attccgcaag acgtttatga ggcagccgaa
     6181 attgatggtt tttctaagtt tcagcaattt cgctatatca ccatgccatt attaaagccc
     6241 acggcgattt ttattttact taccacattg atttcggcct ttaaactaat cattcagcca
     6301 atggtaatga ctcaaggtgg accaatgaat tctactatga caatggttta ttacatttac
     6361 caaactggat ttacagaccg tatggtaggc tactccagct ccattgcgtt aatctttacc
     6421 actatgattg gtttaattac aattgctcaa agaaaattgg taaaggagga tgatccagca
     6481 tgaccaagaa atttggaccg ctaatgatcg tagaatattg tttgctgttc cttttagcgg
     6541 cattattcat tttcccgatg ctgtggatgg tagtttcctc tatgaagcct gaagcagatg
     6601 tctatcaaaa tttagcttcc ttcaaagctt ttttacctag tttgaatcca gctaattggt
     6661 ttaaaactta tcaagaggtt atttcccggt tcagtgtggg aacttattta ttaaacagta
     6721 ttttttatgg tttaaccttt gcctgtgggt caatcattgt aaactctttg gcgggttttg
     6781 cctttgcgaa ggttaatttt tcaggcaaga aaattatctt tggagctttg ttagctctgc
     6841 tgattgtccc gatggagacg gttttaattc cgcaatttac catcgttaat tcactaggct
     6901 tagtgaatac tcgcttggca gtgattttac cagcgatggc cagcgcgttt aatatctatc
     6961 tgtttagaaa tttctttatc gcgattccag aagaaatcat tgaatcagcg aaattagatg
     7021 gtgcaaatat tgttaaaatt ttctttcaca tcatgctgcc aatgtctaaa ccagccatag
     7081 ccacagtagg tgtcttatcc tttatctcca gctggaatga ttatatttgg ccgttaatgg
     7141 tcttaactga cagcagcaaa ttttcaatgc aagtggcaat tactacgatc aatactacac
     7201 agccagttta tatcaatcaa gtgatggctg ttttaaccat tgcaacgatt ccactgatta
     7261 ttgtttatat cgttgcacaa aaatatattc tgcagggact tggcggttct ggaacgggta
     7321 taaaatagga gtgaatagtg aatgttgcaa aaacttttaa aggccaatga gtttattgag
     7381 aaaaatgctc aaacagtcaa tcaagaatac cggaataaat ttcatttgat ggctccgatt
     7441 ggctggatga atgatccaaa tggttttgtt tattatcaag aggagtatca cttgttttat
     7501 caatattatc cttacgacag catctgggga ccaatgcatt ggggccatgc taaaagcaag
     7561 gatctaattc actgggagca tttgccagta gcactggcac caggtgaaag ctatgacaag
     7621 gaaggctgtt tttccggcag tgccatagaa aaggatggca agctgtattt gatttataca
     7681 ggacacaatg ttgtggaagg gcaagttcga caagttcagt gtttggcagt ttcagaagat
     7741 ggcattcact ttgaaaaata tgccggaaat ccaatcatcg cagaagatca cttagcttct
     7801 gttgcaacga ccgaagattt ccgcgatccc aaagtgtttc agcgaaaaga agactatttt
     7861 actgtagtcg ctactaaaac tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa
     7921 gatttactaa cttgggagtt ttattccgta ttgctggagg gaactccaga acaaggcatt
     7981 gtatgggaat gcccagactt gtttgaacta gatggcaaag aagtcttgat tctttctcct
     8041 attcaaatga ctccacaagg caatgcttac caaaatacga actcaactgt tgctttcatc
     8101 ggtactgtcg attggcaaac aggcaaattt ctagtagaga attttcatga aatcgatggc
     8161 ggtttagatt tttatgcacc ccagacctgt gaaaacgaag caggccaaag aataatgatt
     8221 gcttggatgc aaatgtggca gcgtaaaata cctacgaatg atctgggcca tggttggtca
     8281 ggttccatga ccttgcctag agagctgagg gtggctgata atcaattgtt gcaacggcca
     8341 gtttcatcaa tttatcaagt aattgatccg caggaaagga ttgaatgggc gaagactgaa
     8401 gagaatcgct tcttactgaa ggacttagta tatgaccagc actatttacg cttggaaatc
     8461 aatttaaaac aggcaacgaa actagaactt cactatgcga agggacccga ccatacatta
     8521 ctgcttaact atgatgttga acgggagtgc tttactgctt ccagggaaca catgccgtat
     8581 gatttatcag gagctgaaaa ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa
     8641 aataattgtc taaccatcga aattttccgt gatacttcag ctattgagct ttttctaaat
     8701 gatcgtctga ctatgagtac aactttttat gagacagaaa aaggacagga ttttcttttg
     8761 ctggcagaca gtgaagtaac cttttcattg gaaagcggct taattaatct atagaaggga
     8821 gagctcagtg atgatttatg gttcaatcga agcaggcggc acaaaatttg tgtgtgccat
     8881 tagcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga cacctgagga
     8941 aacaatacct ttagtcattg acttcttcaa acaataccaa gcagatttag cagggattgg
     9001 gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg ggtacattac
     9061 ctcgaccccg aaa
//
LOCUS       DAYYHB010000095         8971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000095 DAYYHB010000000
VERSION     DAYYHB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.097"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..651
                     /locus_tag="ACHA6B_002282"
     CDS             55..651
                     /locus_tag="ACHA6B_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8949830.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            632..1078
                     /locus_tag="ACHA6B_002283"
     CDS             632..1078
                     /locus_tag="ACHA6B_002283"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8949831.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1421..1744
                     /gene="relB"
                     /locus_tag="ACHA6B_002284"
     CDS             1421..1744
                     /gene="relB"
                     /locus_tag="ACHA6B_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8949832.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2064..2291
                     /locus_tag="ACHA6B_002285"
     CDS             2064..2291
                     /locus_tag="ACHA6B_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8949833.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2307..3473
                     /gene="hemW"
                     /locus_tag="ACHA6B_002286"
     CDS             2307..3473
                     /gene="hemW"
                     /locus_tag="ACHA6B_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8949834.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3708..4751
                     /gene="hrcA"
                     /locus_tag="ACHA6B_002287"
     CDS             3708..4751
                     /gene="hrcA"
                     /locus_tag="ACHA6B_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8949835.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4845..5408
                     /gene="grpE"
                     /locus_tag="ACHA6B_002288"
     CDS             4845..5408
                     /gene="grpE"
                     /locus_tag="ACHA6B_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8949836.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5459..7288
                     /gene="dnaK"
                     /locus_tag="ACHA6B_002289"
     CDS             5459..7288
                     /gene="dnaK"
                     /locus_tag="ACHA6B_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8949837.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7439..8605
                     /gene="dnaJ"
                     /locus_tag="ACHA6B_002290"
     CDS             7439..8605
                     /gene="dnaJ"
                     /locus_tag="ACHA6B_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8949838.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3128 a         1458 c         1938 g         2447 t
ORIGIN      
        1 atttttcaag ttatctaatg caattatatt gatatcatta ggagctgcat tgttatgaat
       61 ccaacattga ttactaagaa agaattacta aaaaaattag atatttctac tggtgtatta
      121 gctaatttaa ttagaaatgg catgccaaaa gagggagaaa tgtttaatct tgataagatt
      181 attacatggc gagaaaattg gtctaaaaac atattaggtg agttagaagt tggtagagta
      241 tatactaaca aagaaatttc agagaagttt aaatgttcca aacagggtgg aatgagaaga
      301 tctcatcaaa ctaatactct tgtactgttt agtgatcaaa caggaagtaa tgtttataaa
      361 gataagtggt taaatggtat tttacaatat accggtatgg ggctaaaagg ggatcaagta
      421 ttggataaga atcaaaataa agttttagcc aattcaaaaa gtaattttgt aaagattcat
      481 ttatttgaaa catttaaacc aaaagaacac acatatctgg gagaagtata tttagcagga
      541 caaatatata ctgttaatga aaaagatagt tcgggcaaca gcagaaaagt atataaattt
      601 ccattagctt taataaatca agagcaattg attgaagata aagatatata accaggaaga
      661 aaatcaaact agacatattc gtaatttatc taatgctaaa ttagaggaag aagcaagaaa
      721 agttagtaat tataataaaa aaatggctgc aaaatatgtt aacgaagagg agttttgctt
      781 ctcaaaaaca aaaatatata aacgtaatcc cttcgtatct gaatatgtta aacgacttgc
      841 aaaaggtatt tgtcaacttt gccaagaaaa agggccattt ataaaagacg gtgtacctta
      901 tttacattgt catcatatag aatatttgtc gcaaggtgga aaagatgtga ttgaaaactg
      961 tatcgcgtta tgtcccaatt gtcatgctag aattcatgaa cttgagttac ctgacgataa
     1021 agaaaaattg ctgaagatcg ttgaaaaaag agaaaatagc ttttctaaaa taccgtaatt
     1081 tatttttata ggacaaataa gctaatgtga gctatgcctt ctatataaga ctaagttcta
     1141 ttataagtaa cgaagtaatt ctcgttcctt cctgtagtaa tattttttaa ataacctata
     1201 ggcttcttca aaagtacatc ctccagtaaa ttctaagtac tggaggattc tttttctagt
     1261 gttgctttac tcatgaaaaa gtagttggct acttcatcaa aatttctgaa cttttttaat
     1321 gtataataaa gagctcgatc atttactaca aggatgctag ctcctacatt ttcacagttt
     1381 tttagcaagt aaatttttct aaaatcacat caggaaatgc atgagattct ttaagagagg
     1441 caattatagg gctatgatta cggttcgtgt aagtgacgcc aaaaaagaat ggctttctta
     1501 tatggctgaa ttttatggga ttactttatc tgaacttcta aaaaactatt cgatggaaca
     1561 actagaagat gaatacaatc gtcaaacggc tgaaattgcc cataaacatt ggataagaag
     1621 tgggaaaaca acagtattca tggaagaatc cttaatgaat ttggtgggct ggaatgacct
     1681 gccgtttaaa attcacgccc gaaattcaga agcagctctg attggcaaat acgtgtattg
     1741 gtagagatag aagataatca attcgttgtt atcgctattc aagtagggca ccgaagaaat
     1801 gtctatgatt aagagactgt ttatttttac aaaaaaataa taattggaat ctagttaaaa
     1861 ctcttgaagt gagagatttg actagatttt tttatgaata aaataaggac aaaattgttt
     1921 aggaagaatc atgagtgtaa aaaaatcatc caagaagtcg attcttggat tttttagtgg
     1981 atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg
     2041 tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact
     2101 taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta
     2161 gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg
     2221 cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact
     2281 tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg
     2341 tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt gactttaata
     2401 aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga
     2461 gattaatgct ggaaagtcat cctacagcga atttggaaac actttatgta ggaggcggaa
     2521 caccaacttc tttatctgct aaacaacttg atcgactatt atcaggtgct agagagatcc
     2581 tgccttttca aaatggtaat gagtttactg tagaagcgaa tcctggtgat ttgacccgtg
     2641 aaaaacttca agtgatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat
     2701 ttgacaatcg cttattgaaa aaaattggac gtaaacatac tgcagaagat gtgtatcaaa
     2761 caatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat
     2821 taccgggaca aacactagaa ggatatcggg atactttgga aaaagctttg gctttggatc
     2881 tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg
     2941 tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga
     3001 cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct
     3061 ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg
     3121 gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac
     3181 actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac
     3241 gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta
     3301 aaaaacattt ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa
     3361 gggaattaga gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa
     3421 gagggacttt tttggggaac aatgtgtttg agcgcttttt atttgaaaaa tagtttgaag
     3481 aatatttgtt ttttatcgaa acttgaaaag aaaaagttta gaaagtttga caatgaggta
     3541 ttgaagtatt gatataactg gatttacatc aattatttag aattagcact cttgtgttta
     3601 gagtgctaaa aaatggagtc tttctcttga caggagaagg gcttcttgct atatttataa
     3661 gtgaattagc acttagaata tatgagtgct aatgagaggt gaggaagatg ttaacaaagc
     3721 gacaaaacga cattttacgt ctgatcatcc aacactatac gagtagtggc gtgcccgttg
     3781 gttccaaaac attaatgtct gagggagtca aggccagctc agcaacgatt cgcaatgata
     3841 tgaaagcatt agaagatgaa ggattgctgt tgaagactca ctcttcttct gggcggattc
     3901 catctgcttt aggctatcgt tattacgtgg atcatttgtt aaaacctgct cgcgtagcag
     3961 aagatgattt gctgctgatt cgtcagtctc taggcagaga atttcatgaa atcaatgaga
     4021 ttattaagca atctgctgag atattatctg agctgacaag ctatacgact ttttcattgg
     4081 gacctgaaat aaaagaccgc cgactgactg gctttcgtat cgtacccttg aataatcgac
     4141 agattattgc tatcgtcgta acagatatgg ggaatgtaga aagccaagtg ttcactcttc
     4201 ccgaatatat gggaagtcag gaactagaaa aaatggtaca gattgtaaac gaccgtctag
     4261 tcggagatcc tctgatgacg gtgtaccata aacttcgtac ggagattccg atgattcttc
     4321 ataaatattt ccagacgaca gaagggatgt ctcagttatt tgacaccgtt cttggtcatg
     4381 tttttgaaga aaaggtttat gtcagtggac aaatgaatct cttggattat gagccccgtc
     4441 aagatcttga tcagttcaaa tcgatgtttt cttttatgaa agactcagac ggacttacac
     4501 aaatgatcac tccgatggat agcaatattc atatcaagat tggtgctgaa ttaggcaatg
     4561 atttattgca gaatatgagt atgattcaag caagttacga gatagatggt catggaagag
     4621 gtacaatagc cttgttaggg cctactagta tgccttattc tcggatgttc ggtctagtcg
     4681 atgtttatcg aagagaacta gcagctaaac ttgctgatta ctaccgctcg cttgacttgt
     4741 caaactcgta atgtgaataa cgaagctatt tgcagctgat cgttctctct ttcttagtaa
     4801 gagcaatcaa acagctgact gaataatgaa aggaagactg tatcgtgaaa gaaaatcaag
     4861 aagaactaga taaagaaatg acagatgctc agccagaacc ggaaatcgat gtcgaagctg
     4921 cagaagattc gggtatttca gaagctgaag cggaagaatt cgaaacagcg aaattaaaag
     4981 ctgagttaga agaaatggaa gataaatact tgcgggctcg tgctgaaatc gcaaatatgg
     5041 caaatcgagg aaaaaatgag cgtgagcaat tacaaaaata ccgttcacaa gatttagcca
     5101 aaaaattact tccatcaatt gacaacctgg aacgtgcatt agccacagaa gtgtcagatg
     5161 atcaaggggc aggtttgaaa aaaggcgtgg aaatggtctt ggaaagtttg cgtaacgcac
     5221 tggaagaaga aggaattgaa aaaatccctg ctaaaggaga agcctttgat cctaatcttc
     5281 atcaagcagt acaaactgtt ccagcgactg aagatacacc agccgatacg attgtagaag
     5341 tactacaaga aggctacaaa ttacatgatc gcgtgttaag accaactatg gtcattgttg
     5401 cacaataatt gtgcggatat aaaacttaca ctttcaatta aagaaggaga cgttaattat
     5461 gagtaaaatt atcggtattg acttaggtac gactaactct gcagtagcag tattggaagg
     5521 aaacgaagca aaaatcatcg ctaacccaga aggaaaccgt acaacacctt ctgttgtatc
     5581 attcaaaaat ggagaaatcc aagtagggga agttgcaaaa cgtcaagctg tgacaaaccc
     5641 aaatacgatt tcatcaatca aacgccatat cggtgaagca ggttacaaag tagatgtaga
     5701 aggtaaatca tatacaccac aagaaatctc agcaatgatc cttcaatata tcaaaggatt
     5761 cgcagaagac tatttaggtg aaaaagtcga aaaagcagtt atcactgtgc cagcttactt
     5821 taatgatgca caacgtcaag caacaaaaga tgctggtaag atcgctggtc tagaagtaga
     5881 acgtatcgtc aatgaaccaa ctgcagctgc tcttgcctat ggtttagata aaacagatcg
     5941 cgacgaaaaa atcttagtat tcgaccttgg tggcggtact tttgacgtat ctatccttga
     6001 actaggagac ggtgtctttg atgtattgtc aacagctggc gacaaccatc taggtgggga
     6061 cgactttgat aacaaaatca tcgactacat ggtagcagaa ttcaaaaaag aaaacagcat
     6121 tgacttgtct caagacaaaa tggctttaca acgcttgaaa gacgctgctg aaaaagcgaa
     6181 aaaagattta tctggcgtat caagcacaca aatcagcttg ccatttatca cagcaggaga
     6241 atctggacca ttgcatttgg aaatgacatt gacacgtgca aaatttgatg aattaacagc
     6301 tgacttagta gaacgtacaa aagtaccagt acgtcaagcg ttaaaagatg caggtctgtc
     6361 acaatcagaa atcgacgaag ttattttagt cggtggatct actcgtatcc ctgcagttgt
     6421 agaagcagta cgtaaagaaa caggaaaaga accaaacaaa tcagttaacc ctgatgaagt
     6481 agttgctatg ggtgctgcta tccaaggtgg cgtgatcact ggtgatgtga aagatgttgt
     6541 tttacttgac gttacaccat tgtcattagg tatcgaaaca atgggtggag tgttcactaa
     6601 attgattgac cgcaatacaa cgattccaac aagtaaatca caagtattct caactgcggc
     6661 agataatcaa ccagcagtag acatccatgt attacaaggt gaacgcccaa tggctgctga
     6721 caacaaaact ctaggaagat tccaattgac agatattcca gcagctcctc gtggtattcc
     6781 acaaatcgaa gtaacattcg atattgataa aaacgggatt gtaaatgtat ctgcgaaaga
     6841 tctaggtaca caaaaagaac aaaaaatcac gatcaaatct tcttcaggtc taacagacga
     6901 agaaatcgaa cgtatggtga aagatgcaga agcaaacgct gaagcagata aagctcgtaa
     6961 agaggaagtt gacctacgta atgatgtaga cgcattgcta ttctctgtgg acaaaacatt
     7021 gaaagaatta gaaggcaaag tggacgaaga agaagtgaaa aaagcagaaa cagctcgtga
     7081 cgaattaaaa gccgctgttg aagcaaacaa catcgaggaa atgaaaacta aacgcgatgc
     7141 attgaacgaa atcgttcaaa acttgactgt taaactatac gaacaagctg cacaacaaca
     7201 agctcaagaa aatccagaag cagctcaagg cggagcagat gatgtcgttg atgctgattt
     7261 tgaagaagtc gatggcgacg ataaataatc agttcactga atttcgagag aaggccaaag
     7321 ttgctacttt ggctttttct tttgaaaaat agtttgaagt atcggttttt gtttcaaaca
     7381 aaatatgata tgataacaag cgacgtgaaa atgatgagaa ccaataggag gaaagcctat
     7441 ggcaacaaaa cgtgattatt atgaagtctt agggctctca aaaggagcat cagaagatga
     7501 aataaagaaa gcttatcgta agctttcgaa aaaataccat cctgacatca ataaagaacc
     7561 tgatgcggaa gaaaaattca aagaagtatc agaagcatat gagattttaa gtgacccgca
     7621 aaaacgcgct gcatacgacc aatacgggca tgcaggcaca gatccaaatt acggtgctgg
     7681 tggagctggt ggcggatttg gtggattcgg cggcttctct ggtggcggct tcggtggttt
     7741 tgaagatatc ttcgactctt tctttggagg aggcggtcgg tcagtcgatc caaatgcgcc
     7801 tcgtcaaggt gctgatttgc aatatacggt cgacttgtct tttgaagaag cgatttttgg
     7861 tgtagaaaaa gaaatcaagt ataatcggga agaaatctgt catacttgtg gtggaaacgg
     7921 tgcaaaacca ggcacacaac caactacttg tcataaatgt catggtgcag gtacgatcaa
     7981 tgtagaacgc caaacgccac ttggacgagt gatgagccgt caaacttgtg atgtttgtca
     8041 tggcacaggg aaagaaatca aagaaccttg tccaacctgt catggctcag gacatgagaa
     8101 gaaagcacat tcagttaaag tgaatgtacc agctggtgta gaagaaggac aacaaatgcg
     8161 tttagctggt caaggagaag ctggagaaaa cggcggacca tttggtgatt tgtatgtggt
     8221 ttttcgtgta gaagaaagtg atatctttga tcgtgaaggt tctgaaattt actatgaatt
     8281 gcctttaagt tttgtacaag cagctttggg ggatgaagtc caagtaccaa ctgtccatgg
     8341 agatgtgaaa ttgaagatcc ctgctggaac acagacaggt acgaacttcc gtttacgtgg
     8401 aaaaggcgct ccgaagttac gtggtggtgc aacaggcgac caacatgtca aagtaaaact
     8461 gatcacaccg aaaaatctaa atgaacaaca aagagaagca ttacgtgcat tcgctgaagc
     8521 tggcggcatc aaagttgaag aacagcaaga agacggtttc ttcgataagt ttaaagatgc
     8581 ttttggcggg aaaaagcgga agtagttttt ctagtaaaaa tataaagtag acttgcccaa
     8641 attttctatt tctagtaaaa acgctaagtc attaataatt aaaaaccaga cgaacaatca
     8701 tacgttcgcc tggttttttt gtagttgtca tgtttgtacc aacaaaaaag ccagctttag
     8761 aataagataa tcatcatagt ttctgaaggt ggtatcgact atgtacttga tatcgtcaat
     8821 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
     8881 gaacaagcga tgcggctggt acagttaagt gaaaaaacaa gaaactaaaa atgcccggtc
     8941 gtcatgaagc gaccgggcat ttttggtttt t
//
LOCUS       DAYYHB010000096         8941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000096 DAYYHB010000000
VERSION     DAYYHB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.098"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHA6B_002291"
     CDS             complement(13..1152)
                     /locus_tag="ACHA6B_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8949839.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA6B_002292"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA6B_002292"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8949840.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA6B_002293"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA6B_002293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8949841.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA6B_002294"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA6B_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8949842.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA6B_002295"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA6B_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8949843.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHA6B_002296"
     CDS             complement(6166..7257)
                     /locus_tag="ACHA6B_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8949844.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA6B_002297"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA6B_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8949845.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHA6B_002298"
     tRNA            8800..8871
                     /locus_tag="ACHA6B_002298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2318 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 a
//
LOCUS       DAYYHB010000097         8792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000097 DAYYHB010000000
VERSION     DAYYHB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.099"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1094
                     /locus_tag="ACHA6B_002299"
     CDS             33..1094
                     /locus_tag="ACHA6B_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8949846.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACHA6B_002300"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACHA6B_002300"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8949847.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACHA6B_002301"
     CDS             2007..3989
                     /locus_tag="ACHA6B_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8949848.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACHA6B_002302"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACHA6B_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8949849.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ACHA6B_002303"
     CDS             4711..6135
                     /locus_tag="ACHA6B_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8949850.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ACHA6B_002304"
     CDS             complement(6316..7089)
                     /locus_tag="ACHA6B_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8949851.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="ACHA6B_002305"
     CDS             complement(7086..7991)
                     /locus_tag="ACHA6B_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949852.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ACHA6B_002306"
     CDS             complement(8145..8465)
                     /locus_tag="ACHA6B_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8949853.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2764 a         1580 c         1873 g         2575 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     8761 tattatcggg caataaatta aaaaaagctt ta
//
LOCUS       DAYYHB010000098         8542 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000098 DAYYHB010000000
VERSION     DAYYHB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.100"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHA6B_002307"
     CDS             <1..888
                     /locus_tag="ACHA6B_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8949854.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..1626)
                     /locus_tag="ACHA6B_002308"
                     /pseudo
     CDS             complement(928..1626)
                     /locus_tag="ACHA6B_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1615..1881
                     /locus_tag="ACHA6B_002309"
     CDS             1615..1881
                     /locus_tag="ACHA6B_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949855.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKGFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2057..2626)
                     /locus_tag="ACHA6B_002310"
     CDS             complement(2057..2626)
                     /locus_tag="ACHA6B_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8949856.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2700..3023)
                     /locus_tag="ACHA6B_002311"
     CDS             complement(2700..3023)
                     /locus_tag="ACHA6B_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949857.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3177..3989)
                     /locus_tag="ACHA6B_002312"
     CDS             complement(3177..3989)
                     /locus_tag="ACHA6B_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949858.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4316..5191
                     /locus_tag="ACHA6B_002313"
     CDS             4316..5191
                     /locus_tag="ACHA6B_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8949859.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            5205..5528
                     /locus_tag="ACHA6B_002314"
     CDS             5205..5528
                     /locus_tag="ACHA6B_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949860.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            5525..6277
                     /locus_tag="ACHA6B_002315"
     CDS             5525..6277
                     /locus_tag="ACHA6B_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8949861.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6267..8438
                     /locus_tag="ACHA6B_002316"
                     /pseudo
     CDS             6267..8438
                     /locus_tag="ACHA6B_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2664 a         1632 c         1521 g         2725 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt
      961 gtcatgaaaa tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat
     1021 catgacgtat actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac
     1081 atttcctaaa gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac
     1141 tctagctagt tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt
     1201 agcaatgtgc catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat
     1261 gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc
     1321 aattactggt ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc
     1381 ataggctagt ttttctttac ttacacctat ttcagaaaga taaccgtaag gatctgtatg
     1441 atcgccccaa atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg
     1501 agtgtctaat gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta
     1561 tactttatag ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac
     1621 aggacttttg gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg
     1681 aaaacaccaa cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta
     1741 gaagatatca aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat
     1801 atttctcccg aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa
     1861 ataaaaaagc actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa
     1921 ttttatttat aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt
     1981 atcttaaagg cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag
     2041 tgcgaacatg gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca
     2101 tcaattgaga ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg
     2161 gttttcttct taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca
     2221 cgactccgcc aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta
     2281 gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct
     2341 cttccatccc ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta
     2401 tttcttttgg ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca
     2461 agagcgtttc gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata
     2521 tatatacgcc agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca
     2581 cttctgtgat cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt
     2641 tactaatcgc tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt
     2701 tatcggtttt tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa
     2761 ggatggtaca gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg
     2821 atcactagat ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt
     2881 tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca
     2941 tagcgaaaaa aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc
     3001 tggagtgttt tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg
     3061 atcgctagcc gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc
     3121 tgataataac gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat
     3181 tcttggtaaa gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc
     3241 caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct
     3301 tctatccgtt tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga
     3361 taattttttt cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat
     3421 tcaaagaata gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc
     3481 ttcaataatt ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc
     3541 ggaaaatctt tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc
     3601 tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct
     3661 tgcgtagaac gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa
     3721 caaaacgggg gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga
     3781 tcataaacta actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc
     3841 gccaaacgtt tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa
     3901 tcagcttttc gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca
     3961 ttttttgata atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat
     4021 tgtttgatca aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa
     4081 atacgcaaat cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa
     4141 aacgaatgta ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc
     4201 cattacaatg aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat
     4261 agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg
     4321 caaacgttca actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt
     4381 cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca
     4441 gggaaccttc agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt
     4501 ttccgtatac aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac
     4561 gatcttgtta gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga
     4621 agcaatggcc gagaatcatg caacgatcga caacaataca taccctctag acaatcattt
     4681 ttttgtctta gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc
     4741 acagctcgat cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact
     4801 tttgctactt ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag
     4861 ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc
     4921 atcttatgcc ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat
     4981 cagtccacga ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg
     5041 gagaaattac gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg
     5101 cctgattttt tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct
     5161 tgttgcacaa attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag
     5221 attatttcat taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg
     5281 tattcaacaa ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga
     5341 gcttgatttc attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac
     5401 tcgtttatcg caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt
     5461 ctttacctct ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga
     5521 gattttgatt cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag
     5581 aggatgccat gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag
     5641 aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat
     5701 tcaagcagaa aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag
     5761 aggtcttgat tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga
     5821 ttggaaattg tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga
     5881 agaatgttct cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct
     5941 agacttgtat ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt
     6001 aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc
     6061 tggcgatgag aaaacttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct
     6121 ctttgttcct gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg
     6181 aacggtttat cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat
     6241 atgttcattg ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg
     6301 tttactcctt tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc
     6361 aaaacagcta ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc
     6421 ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat
     6481 attttccttc gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag
     6541 aggaattctt ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt
     6601 ttctcataat gttgctgatc tatttattta ttacattgac cgtggattac gactattggt
     6661 acgcaccctt tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag
     6721 atttactgta cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc
     6781 aatttttctc catccggttg ttgtcgagaa acactcgatt ccagtcattg actacagttc
     6841 tgcttatttg tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc
     6901 aatctctcga ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg
     6961 acacactcca tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg
     7021 aacagcttgg tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag
     7081 gaaatggcta ttggaagatc gaaaataaaa atcattacac cggttctgga tgaatagaag
     7141 atcaggacgc acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa
     7201 ccgttataga agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt
     7261 atccttatgg aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact
     7321 atttgcctga aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt
     7381 tacaaacaga aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg
     7441 atgatttaga aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg
     7501 aggacttggc ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg
     7561 tctaacagta tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata
     7621 ctccaaaaga acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg
     7681 acaacttttc aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga
     7741 caaaaggttt tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca
     7801 gcattctcaa tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc
     7861 ttccatttga accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa
     7921 caaccgaaac cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa
     7981 ctatttccag tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt
     8041 ataaaatcgt cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt
     8101 tattcagaaa atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta
     8161 ctaatacgat tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac
     8221 ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc
     8281 gtttcattcg attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg
     8341 taaaattcga taaaaaaacc tatttagaaa taacgcatat aataagaaaa acggtataca
     8401 aaaaaattct gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta
     8461 cttctcgaat gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac
     8521 acgttttttg tcacaacctt tt
//
LOCUS       DAYYHB010000099         8526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000099 DAYYHB010000000
VERSION     DAYYHB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.101"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1395)
                     /gene="tyrS"
                     /locus_tag="ACHA6B_002317"
     CDS             complement(139..1395)
                     /gene="tyrS"
                     /locus_tag="ACHA6B_002317"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8949862.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1507..1712)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1975..3276
                     /locus_tag="ACHA6B_002318"
     CDS             1975..3276
                     /locus_tag="ACHA6B_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8949863.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3345..3944)
                     /locus_tag="ACHA6B_002319"
     CDS             complement(3345..3944)
                     /locus_tag="ACHA6B_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8949864.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4198..5079
                     /locus_tag="ACHA6B_002320"
     CDS             4198..5079
                     /locus_tag="ACHA6B_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8949865.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5092..6702
                     /locus_tag="ACHA6B_002321"
     CDS             5092..6702
                     /locus_tag="ACHA6B_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949866.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6779..7438)
                     /locus_tag="ACHA6B_002322"
     CDS             complement(6779..7438)
                     /locus_tag="ACHA6B_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8949867.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7721..8386)
                     /locus_tag="ACHA6B_002323"
     CDS             complement(7721..8386)
                     /locus_tag="ACHA6B_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8949868.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         2436 a         1666 c         1574 g         2850 t
ORIGIN      
        1 aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc
       61 gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag
      121 gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt tcttaccttt
      181 tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa aatttaaatc
      241 ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac gtgcttgacg
      301 tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag gtgtgcttga
      361 aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt
      421 aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt cttcttcact
      481 atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt tctcaggttc
      541 tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag taaagaattt
      601 caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga actcaaatgg
      661 tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga atttcgttcc
      721 gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg gtccttctaa
      781 ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc caccgatttg
      841 caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca ggatttggta
      901 agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat cttttgctaa
      961 catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta aagagatttt
     1021 tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa acaattttct
     1081 catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt gcaacacacg
     1141 ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata
     1201 tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca aatgtccgat
     1261 atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct tttcttcaac
     1321 gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc
     1381 atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca ggaacctgct
     1441 agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag tcccttggca
     1501 tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag atatccaaca
     1561 tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta
     1621 attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa cattggcttt
     1681 aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta tcatagagcg
     1741 caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat tttttctttt
     1801 ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga aataactttt
     1861 tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta ttctttcttc
     1921 gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa
     1981 aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat tttaattcta
     2041 agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa
     2101 ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac tcaagcaaaa
     2161 aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa agatagtaag
     2221 aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa agaacaactg
     2281 atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt gatcgtggac
     2341 ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat ggtgactgtt
     2401 gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt agttaatttg
     2461 aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct tgcgctacct
     2521 gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga atatatggat
     2581 aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa aaatgtctat
     2641 aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat cgatgccttt
     2701 gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt
     2761 tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa tgaaaaaaca
     2821 aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga cggctggagc
     2881 tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga agatgtaaaa
     2941 aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac aaatattctg
     3001 atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga aaatcaaaaa
     3061 tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc ttctacacca
     3121 gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat cgatggcatt
     3181 cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat caatgtccat
     3241 gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta tgaaccaaaa
     3301 aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt cttcttcggt
     3361 gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag ttttgcgtat
     3421 atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa gaactcatcc
     3481 gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc aagcatgata
     3541 ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt ttcttttttt
     3601 gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag ataacggatt
     3661 ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc aatcaacgct
     3721 ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat cagcccttcc
     3781 ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc agctacctta
     3841 tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa
     3901 atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt
     3961 aattttaaac ttttataata tatatagttc aaatttattc tgaggtttat tataactatc
     4021 tattgagaaa gtatcaagct agcaattcct tgtttatgaa ttcattgaca gaaatttata
     4081 aaaagaatat tatgtactaa aataataaaa aaagttttat aaaaaggtat aaaacctttt
     4141 tattaaacta tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg
     4201 gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa
     4261 gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct
     4321 ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca
     4381 acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat
     4441 gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc
     4501 atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct
     4561 cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg
     4621 attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta
     4681 gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa
     4741 tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg
     4801 atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact
     4861 cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt
     4921 gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc
     4981 gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg
     5041 actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat
     5101 aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc
     5161 ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa
     5221 ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt
     5281 gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat
     5341 gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg
     5401 gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga
     5461 gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt
     5521 ttagcaatat cgatcagctg tacgatgatt tttttccaag cggattcgat cactgcagaa
     5581 ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca
     5641 ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt
     5701 ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg
     5761 tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct
     5821 ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa
     5881 catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt
     5941 tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg
     6001 cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag
     6061 tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa
     6121 aaaaacttta caagcacgat catggtagtc ttgatttgtc tagctgctat tttaccaatt
     6181 gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca
     6241 acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat tctatttgct
     6301 atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag
     6361 gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc
     6421 gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa
     6481 cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat
     6541 ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat
     6601 tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt
     6661 ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat
     6721 ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt
     6781 acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat
     6841 aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa
     6901 atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag
     6961 ctaagtcaag cagatagaga gcgactcgtt cttctacttt ttccatcatc aaaaaagttg
     7021 tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg
     7081 cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt
     7141 tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct
     7201 catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat
     7261 acactttcat actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa
     7321 tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt
     7381 gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa
     7441 tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact
     7501 gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg
     7561 gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct
     7621 atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg
     7681 agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac
     7741 ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt
     7801 gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg
     7861 cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt
     7921 cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc
     7981 tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt
     8041 cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg
     8101 ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc
     8161 gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag
     8221 gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt
     8281 ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt
     8341 agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt
     8401 ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa
     8461 gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca
     8521 gtttta
//
LOCUS       DAYYHB010000100         8513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000100 DAYYHB010000000
VERSION     DAYYHB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.102"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..333
                     /locus_tag="ACHA6B_002324"
     CDS             46..333
                     /locus_tag="ACHA6B_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949869.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            749..2287
                     /locus_tag="ACHA6B_002325"
     CDS             749..2287
                     /locus_tag="ACHA6B_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8949870.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2284..3249
                     /locus_tag="ACHA6B_002326"
     CDS             2284..3249
                     /locus_tag="ACHA6B_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949871.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3236..4558
                     /locus_tag="ACHA6B_002327"
     CDS             3236..4558
                     /locus_tag="ACHA6B_002327"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8949872.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4621..4725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4799..5542
                     /locus_tag="ACHA6B_002328"
     CDS             4799..5542
                     /locus_tag="ACHA6B_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8949873.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5557..6549
                     /locus_tag="ACHA6B_002329"
     CDS             5557..6549
                     /locus_tag="ACHA6B_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8949874.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6561..7298
                     /locus_tag="ACHA6B_002330"
     CDS             6561..7298
                     /locus_tag="ACHA6B_002330"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949875.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7288..8010
                     /locus_tag="ACHA6B_002331"
     CDS             7288..8010
                     /locus_tag="ACHA6B_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8949876.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8094..>8513)
                     /locus_tag="ACHA6B_002332"
     CDS             complement(8094..>8513)
                     /locus_tag="ACHA6B_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8949877.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2745 a         1463 c         1835 g         2470 t
ORIGIN      
        1 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
       61 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
      121 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
      181 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
      241 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
      301 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
      361 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
      421 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
      481 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
      541 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
      601 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
      661 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
      721 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
      781 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
      841 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
      901 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
      961 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     1021 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     1081 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     1141 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     1201 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     1261 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     1321 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     1381 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     1441 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     1501 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     1561 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     1621 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     1681 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     1741 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     1801 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     1861 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     1921 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     1981 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     2041 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     2101 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     2161 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     2221 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     2281 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     2341 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     2401 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     2461 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     2521 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     2581 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     2641 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     2701 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     2761 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     2821 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     2881 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     2941 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     3001 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     3061 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     3121 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     3181 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     3241 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     3301 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     3361 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     3421 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     3481 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     3541 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     3601 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     3661 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     3721 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     3781 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     3841 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     3901 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     3961 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     4021 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     4081 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     4141 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     4201 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     4261 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     4321 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     4381 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     4441 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     4501 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     4561 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     4621 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     4681 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     4741 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     4801 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     4861 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     4921 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     4981 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     5041 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     5101 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     5161 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     5221 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     5281 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     5341 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     5401 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     5461 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     5521 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     5581 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     5641 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     5701 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     5761 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     5821 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     5881 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     5941 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     6001 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     6061 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     6121 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     6181 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     6241 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     6301 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     6361 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     6421 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     6481 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     6541 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     6601 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     6661 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     6721 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     6781 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     6841 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     6901 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     6961 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     7021 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     7081 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     7141 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     7201 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     7261 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     7321 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     7381 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     7441 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     7501 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     7561 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     7621 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     7681 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     7741 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     7801 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     7861 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     7921 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     7981 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     8041 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
     8101 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
     8161 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
     8221 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
     8281 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
     8341 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
     8401 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
     8461 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAYYHB010000101         8327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000101 DAYYHB010000000
VERSION     DAYYHB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.103"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA6B_002333"
     CDS             476..1339
                     /locus_tag="ACHA6B_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8949878.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA6B_002334"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA6B_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8949879.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA6B_002335"
     CDS             3339..3860
                     /locus_tag="ACHA6B_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8949880.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA6B_002336"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA6B_002336"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8949881.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA6B_002337"
     CDS             5941..6759
                     /locus_tag="ACHA6B_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8949882.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA6B_002338"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA6B_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949883.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA6B_002339"
     CDS             7228..7806
                     /locus_tag="ACHA6B_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8949884.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA6B_002340"
     CDS             7901..8038
                     /locus_tag="ACHA6B_002340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949885.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2511 a         1489 c         1642 g         2685 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaa
//
LOCUS       DAYYHB010000102         8216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000102 DAYYHB010000000
VERSION     DAYYHB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.104"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      68..147
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            145..1338
                     /gene="metK"
                     /locus_tag="ACHA6B_002341"
     CDS             145..1338
                     /gene="metK"
                     /locus_tag="ACHA6B_002341"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8949886.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1468..2940
                     /locus_tag="ACHA6B_002342"
     CDS             1468..2940
                     /locus_tag="ACHA6B_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8949887.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3023..3811
                     /locus_tag="ACHA6B_002343"
     CDS             3023..3811
                     /locus_tag="ACHA6B_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8949888.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3878..4546)
                     /locus_tag="ACHA6B_002344"
     CDS             complement(3878..4546)
                     /locus_tag="ACHA6B_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8949889.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4661..6373
                     /locus_tag="ACHA6B_002345"
     CDS             4661..6373
                     /locus_tag="ACHA6B_002345"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949890.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6376..8148
                     /locus_tag="ACHA6B_002346"
     CDS             6376..8148
                     /locus_tag="ACHA6B_002346"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949891.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2515 a         1380 c         1807 g         2514 t
ORIGIN      
        1 tttttatatt ttttaagcaa aaaaatgaaa aaatacgagc agaaaactgt tttatctgct
       61 atacttggtt cgttgcaagt tcccgaaagg atccgcagga cggagatgcc ttgtaaccga
      121 atttaactag ggggaatcat tataatggta gaaagacact tatttacatc agaatctgtt
      181 tctgaaggac atccagataa aattgctgac caaatcagtg atgcaatctt ggatgcaatt
      241 ttaaaacaag atccaacagc acgagtagcg tgtgaaacgt ctgtaacaac tggtcttgtt
      301 ttagtatttg gagaaatatc tacgacggca tatgtagata ttcaaaaagt cgtacgggaa
      361 acaataaaag aaattggtta tacacgtgca aaattcggat ttgatggaga tacagcagct
      421 gtattggttg cgattgatga acaatctcct gatattgctc aaggagttga tgaagcactt
      481 gagatccggg atgaagataa aaaagacgta ttagatgaga ttggtgctgg tgaccaaggt
      541 ttaatgtttg ggtttgctgt agatgaaaca ccagaattga tgcctttacc aatcgctttg
      601 agtcatcgtc tggtacgacg cttggcagat ttgcgtaaat caaatgaatt aacgtattta
      661 cgtccagatg caaaatctca agtaacggtt gaatatgatg atcaaggaca accggaacgc
      721 gtagatacaa tcgttatttc aacacagcat gatgatgcag tagataatga aacaattcgt
      781 catgatgtca tcgaaaaagt agtgaaggaa gttattccag ctgaattatt agatgatcaa
      841 acgaaatatt atatcaatcc gactggccga tttgtcattg gtggtcctca aggggatgcc
      901 ggattaacag gaagaaaaat cattgttgat acgtatggcg gttatgctcg tcatggtggc
      961 ggtgcttttt ctggtaaaga tgcgacaaaa gttgaccgtt ctgccagcta tgctgcacgt
     1021 tatattgcta aaaacattgt tgcagcaggg cttgctcgca aagcagaagt acagctagct
     1081 tatgcgattg gtgttgctca acctgtttcg atctcaatca atactttcgg tactggaacg
     1141 gttccggaag aagaactgat tgctgcagtg agagaaaact tcgatcttcg gccagcagga
     1201 atcatcgaga tgcttgattt gcgtcgtccg atttacaaac aaacagctgc ttacggtcat
     1261 tttggtcgta cagatgtaga tttgccttgg gaacatacgg acaaagtaga tgcgttgaaa
     1321 gcaagtttag caaaataagt tatattcagc tggaacgatc gccaagtggt ggttgttcca
     1381 gcttttttta tggcagttaa cctcttgaaa aagatagtaa ttatttatct atttttagat
     1441 cctgtcataa tgaaagggag gaataagatg aaaaaagaaa caaatgttac tttggtcaca
     1501 gtgagtattt tcattgcaac gttcatgacg gctattgaag gtacaatcgt aacaaccgct
     1561 atgccaacga ttgtcggttc attacatgga atggaaatca tgaactgggt attttcgatt
     1621 tatttgctga caaacgctat gcttactccg atttacggaa aacttgcgga taaagtggga
     1681 cggaaaccaa tctttatttt tgggacatta ttgtttatta tcggttcgtt attttgcggt
     1741 ttatctcaaa gcatgattgg gttgattgct gctcgtgcga ttcaagggat gggtgctggg
     1801 gcaatgatgc cagtagcttt gacgatcatc ggcgatcttt attctattga aaagcgagca
     1861 aaagttttag gtttgaatag ttcagcctgg gggattgcca gtgtttttgg accgcttgcg
     1921 ggaggattca tcgttgaaac gatcagctgg cactggatct tttttatcaa tgtgccgatc
     1981 ggaattctat taattatttt gatttggcta tatttaaatg aaccaaaaat catacatgag
     2041 tctaaaccaa tggatatttt tggtagtttt agtttgatgg ccatgcttgt ctcattgctg
     2101 ttagggttcc aattattagg agacgaagga atgagtccgc ttgtttctgc tttgctgcta
     2161 ggtagtatcg taatgttagg tatttttgtt tttgccgaaa aaagagctaa agatcctgtc
     2221 atcatgatgc atttattcaa aaatccacta tttgttgtgg tcaacttggc tgcagcattg
     2281 gctagtggtt ttttgatggg aatcgatgtt tatatcccta tgtggatgca aggtgtatta
     2341 gggctgaatg cagcaattgg tgggctggta ttagcaccat tatcactagt ctggatggtc
     2401 ggttctttcc ttgcaagcaa gtggatgact caaatgaaaa ctcattgggt cttgagaatt
     2461 ggactactaa tcgcagctat tgggggcgct tggttgtatc ttatgccttt cagaagtaac
     2521 tatttatggt tttttgctat atcaacggtc atcggtatag gcttaggtgt gaccatgact
     2581 acctcgactg tcagtgcaca aaatagtgta tctcgtgacc agcttggtgt agcaacttct
     2641 tttaatacgt tagttcgaac aattggtcaa acagtcatgg tttctatttt tggtttattg
     2701 ataaatgggg taactaaacg agaacttgca gcagcaaatt taacaaatga tgcagatatc
     2761 atgaataaac tggtgaatcc acaaacggct aaatatattg atgcggatat tttgttacca
     2821 ttaagaaaaa ttctttatga tgggttacat tccgtttatc tagttggctt gttactcgtg
     2881 atcattgctt tggtcttaac gcttttcgtt catgaaaaaa gtggtaaaat aaaacagtag
     2941 tttcggttaa gatatgtacg tagccgaatc attcaaattt aaagttattc aaaaataact
     3001 tcttactaag gaaggcgaaa atatggcatt tattcaagca aatatttatt cgaatgtgtt
     3061 agaaatggaa gtcaatctaa atgtgatact accgcaaaca acaaaaaagg tgattggcac
     3121 cagttcagag caagagacaa cggatatacc tgttttatat ttacttcatg gaatgggtgg
     3181 caatcatagt gtatgggaaa gacgtacgtc gattgaacgt tatgtagcaa actacggatt
     3241 agctgtcatc atgccatcta ctgatcttgg ctggtacaca gatacagttt atgacatgaa
     3301 gtattggaca ttcattgcag aagagctacc tgagatttgt catgaattat tcccgcaatt
     3361 aacaagaaaa agagaaaaaa catatgccgc tggattatcg atgggcggat atggcgcgtt
     3421 aaaactaggc cttgcaaaat cagaaaattt cgcagcagta atctctcttt caggcgctgt
     3481 ttctgtagga aatcgaatgg atgatctttt gatggttcgt aaagcatctt tttgggaagg
     3541 gatctttgga ccattggatc aaatcgaagg ttcaaaaaat gatccgcaat acttattgaa
     3601 agaactagta aaaagcggaa aaccagttcc aagattttat atggcttgtg gggaatctga
     3661 ttttctttat caggcaaatc aggaaatggt atctgctatg caggaacaag gcttggcggt
     3721 cacctttgaa caaggacctg gcgatcataa ctggatcttc tgggaccaat ggattcaacg
     3781 tgcattggcg tggctaatgg aagaaaaata aaaaaagagt cttaaaaaga tagctcctat
     3841 agagatcaca aacatagtga tctctatagg agctttttta ctgctcgttt tctcttcttt
     3901 tattttgttg gcataagcct tcaatatagg tttgtgcact cgttagaaag agtgcttttt
     3961 cattcaattc tgcatgggac tgcatacttt ctttaaatcc ctctaattga ttttcaacta
     4021 cataattacg cataaataga actgtttctt tcaaaaattc atttccgtcc ataatttttt
     4081 gtcgaagctg ctgttcatgc tgctgatcca ttaactgtcc aatcaataaa ttgtggagag
     4141 aggcaatggc ctgatgactt tgggtgactg aaaatccttc acgaacaagt aattggatca
     4201 tttgctccaa atgttctaat cgactcggaa aagcgggcac agtattgatt tctaattctg
     4261 ccccacaagg gaaacgtgta taaaggtcat agtaattttc catatactgc agcaactgtt
     4321 cattccaagg caaagttgga tctggaagcg ctaattcttc ttccattgtt tcagccattt
     4381 tttgcaagag ctgttgtttg tttttgaaat accaatataa agcaggtgct tgaacatgaa
     4441 ttctgtttgc gatgtttcgc atggaaagct gcgaaagagc ttgtgtttca gccaggatat
     4501 caaaggcggc ctgaatgatg attgcttgag aaagctttat ttccatttta tcaacctcgt
     4561 gtattgcatt atctttttaa acatggtaaa ttgttagtcg gtctaatttt acagtgttaa
     4621 attagtcctg tcaatttaaa caaaggggtg taatcaacga atgaaattaa tcttacgaca
     4681 tgcgaaaaaa tataaagtag ctgtttttat ttccttgctg tccgtagcag tcatggttgc
     4741 agcagcatta tggcaaccta agttactaca gcaagtattg gaagctatta ttacagaaga
     4801 taatgatgaa atgaaaacaa ttggtattta cctgatcagt ctggctttgc taggattagc
     4861 cgcaggtgta acgaacacga ttttctctgc aaaagtagct caaggcgtaa gtgcagatat
     4921 ccgagaagaa gcatttcgaa agatccagac attttcattt ggaaatatcg aacagttctc
     4981 tgctggaaat cttgttgttc gtctaacaaa tgatatcacg caaatccaaa atttagtgat
     5041 gatttctttg caatcacttt ttcgaattcc attcttattt attggtgcct tcattttagc
     5101 tatgatcacg atgccgcaat tatggtggat catcgtagcg cttatcgtta cggtatttct
     5161 gattacggca ctttcattta caaggatggg caagcacttc atgatcattc aaaaactgat
     5221 tgatcgtgtg aatagcattg caaaagaaaa cttactaggg attcgtgtag tgaaatcttt
     5281 tgtacaagaa gaaaaccaat tgaagtcatt cagcaaagta agtgaggact taacaaaaca
     5341 caacatcata gtcggtacat tattttctgt gatgatcccg tccttcatgc tggctgccaa
     5401 tttggctgtg gttggggcga tcttttttgt gagcgatctt gttaaagatg atcctacgtt
     5461 aattggtgga attgcttcat tcatgaacta cttgatgcag attatgatgg caatcattat
     5521 tggcggaatg atgatgatga tgacttcaag agcagccgta tcgctgaagc ggattgcgga
     5581 aattttagat gctgaaccag atcttactta tttagacgtt ccagaacaag agttgacggg
     5641 ttctgtcact tttgaccatg tttcttttcg ttatcctggt gaggatacag atacgcttaa
     5701 agatatcagc ttttctatca aacctggaga aatggttggt atcgtcgggg cgactggtgc
     5761 cgggaagtca acactagcac aattgatccc tcgtctattt gatccgacag aaggcaaagt
     5821 tgaagtaggt ggtgtagatc ttcgtcaagt aaatgaaaag agtttaagac aaacggtttc
     5881 ctttgttttg caaaaagcaa ttttattctc aggaacgatt gctcaaaact tgcgccaagg
     5941 gaaaaagaat gcaacagaaa aagagatgga acatgcttct tctattgctc aagcaaaaga
     6001 attcatcgaa aagttatctg atcgttacga agcaccaata gaagaacgta gcagtaactt
     6061 ttctggtggt caaaagcaac gtttatccat tactcgtggc gttatcggta atccaaagat
     6121 cttgatccta gatgacagta cgagtgcact ggatgctcgc tctgaaaagc tggtccgaga
     6181 agcactcgat cgagatttga agggaacaac gacgattgtc atcgcacaaa aaattgcttc
     6241 tgttgtcaaa gcagaccgca tcttagtatt ggatgaaggc agattagtcg gcgaaggaac
     6301 ccatgaggaa ttggtagctg gaaaccgtgt ctatcaggaa atctttgaaa ctcaaaaagg
     6361 aacggaggag taagtatgac tgatctaata aaagcaagta aatttttcta tcactatttg
     6421 aagcggtaca aactctcctt tttctttatc tttatcacag tgatcattgc tacctatcta
     6481 caagtaaagg cacctcaata catcggggaa gcttttcaag aattagctaa ttacgtagga
     6541 ggcttgatgc aaggcgtaga cgataaaagc aaatttgttt ctgtaatatg gaaactcttg
     6601 ctgttttatg tactagctag tgcagccaac tttatctata gtattttgtt cacacaagta
     6661 gtagggaagt caacgaaccg aatgcgtatc ggtctattca acaaattgga aaaattgacg
     6721 atccgtttct ttgattctca tcaagacggt gaaattttaa gtcgttttac cagtgactta
     6781 gataatattc aaaatagttt gaaccaagct ttgcttcaag tcatcacgaa tgctgtttta
     6841 cttgtcggga tcttgatcat gatgttccgc caaaatgtag agttagcttg ggctacaatt
     6901 gcttccactc cagtagccgt tttgatagct gtcttgatta tccgcaaagc acggaaatat
     6961 gtggatcttc agcaagatga agtggggaaa ctaaatggtt atatggatga aaaaatcagc
     7021 ggacaacgtg tcatcatcac aaatggctta caagaagaaa cgattgaagg attcttagag
     7081 cataatcaaa gtgttcgcga agcaaccttc aaaggacaag cttattctgg tctactattt
     7141 ccgatgatgc aaggaatgtc actggtcaat acagcgatag ttatcttttt cggcggctgg
     7201 ttagcattaa atggtgattt ggaacgttcg gtggcattag gcttagttgt gacattcgtc
     7261 cagtattcac aacaatatta ccaaccactg atgcagattt catctggata cagcatgatc
     7321 caattagcga ttacaggggc aagacgattg aatgaaatat ttgatgaaaa agatgaagtc
     7381 aaaccagaaa acggtaaagc atttgatggt gttgaaaaag gtcttgcgtt gcagcacgta
     7441 gattttggtt attctccaga aaaactagtc ttgaaagatg tctcgatcaa tgtggacaaa
     7501 ggagaaatgg ttgctcttgt tggtccaaca ggatcgggga aaacgacgat catgaatctg
     7561 ctaaaccgtt tttacgatgt caatgatggt tcagtagtca tcgatggaat agatatccga
     7621 gattatgatc ttgatgcatt acgtagacat gtaggaatcg ttttgcaaga atctgtattg
     7681 ttctccggaa cgatcaaaga caatatcaca ttcggcaaac cggacgcttc agatgaagaa
     7741 gtcatcaatg cagcaaaaca agcgaatatt catgacttca tcatgacgtt agaaaaaggt
     7801 tatgatacag agatatccga agaaaataac ttattcagta caggtcaaaa gcagttgatc
     7861 agtattgctc gaacgatcat cacaaatcca tctttattga ttttggatga agcaacaagt
     7921 aatgtagaca cggtaacaga agcaaggatc caaaaagcaa tggatgaagc aatcaaagga
     7981 agaaccagct ttgtgattgc tcaccggctg aaaacaatct tgaatgcaga tagaatcgtt
     8041 gtcttgcgtg atggggaagt cattgaagaa ggcagtcatc atgagttatt agcagaaaat
     8101 ggattttatg cggaacttta tcataatcaa tttgtatttg aaagttaaat taaaagaacc
     8161 tcgttataac ggggttcttt ttatatattt tatgttgttt gtttttataa ggggca
//
LOCUS       DAYYHB010000103         8165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000103 DAYYHB010000000
VERSION     DAYYHB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.105"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..180)
                     /locus_tag="ACHA6B_002347"
     CDS             complement(<1..180)
                     /locus_tag="ACHA6B_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949892.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFL"
     gene            complement(187..735)
                     /locus_tag="ACHA6B_002348"
     CDS             complement(187..735)
                     /locus_tag="ACHA6B_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8949893.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(780..1112)
                     /locus_tag="ACHA6B_002349"
     CDS             complement(780..1112)
                     /locus_tag="ACHA6B_002349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949894.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1126..1356)
                     /locus_tag="ACHA6B_002350"
     CDS             complement(1126..1356)
                     /locus_tag="ACHA6B_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949895.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1578..2120)
                     /locus_tag="ACHA6B_002351"
     CDS             complement(1578..2120)
                     /locus_tag="ACHA6B_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8949896.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2141..3232)
                     /locus_tag="ACHA6B_002352"
     CDS             complement(2141..3232)
                     /locus_tag="ACHA6B_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8949897.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3240..5450)
                     /locus_tag="ACHA6B_002353"
     CDS             complement(3240..5450)
                     /locus_tag="ACHA6B_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8949898.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5466..7994)
                     /locus_tag="ACHA6B_002354"
     CDS             complement(5466..7994)
                     /locus_tag="ACHA6B_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8949899.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
BASE COUNT         2481 a         1792 c         1173 g         2719 t
ORIGIN      
        1 taaaaaaatt aaccaacaaa catcgacaaa cagtaaatga tataatccat ttccacctcc
       61 aagcatattc aatactggtg aacatgcctt agaaacacga atatagttat ggtttaaata
      121 gatattttct ttaaacattg cttgtttaaa agccatatgc tcttgttcta aacttttcat
      181 aaacttttac tctaatgcac agctagcagc agctgctgca attcctgcta caatatttac
      241 cttagctaac tctttagctg cagcttttcc taattgtttt tctgcaattt ttaataataa
      301 atccgcagct ttttcaaaat taaaagcttt taatgcttca ataaaatctc cctgcgtacc
      361 tatagcccaa gccactcctc ctccaggaag agaattaatg gctacacatc cagcatatcg
      421 tttccaatca actttctcca ttctaggaac aataatatgt tcataaaaga ctcttgctgc
      481 tcttccttgt tcgttatctt gactgattcg ttcacgaata gcaccaaaat cctttatatg
      541 gtatacacca tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta attcatctac
      601 taatttattc tttatttcta aatcaactgt attcactttt tctgtggcat acacacttgt
      661 tccagtacta acaaaagtac ttaaacctaa taagaaaaca cctaaaacac caaatatttt
      721 aattattctt ttcataattt ctcctccata aaaaataagt tactttttaa atatcttgtt
      781 tatgaattca ttccccaaag gccttttaag attgatacag cagtccgttc acttctttta
      841 gacttgtcat acaagtcttt taaatgatcc catatcagac tagtttgtct atctagcttc
      901 aatccattaa acgctaatga acggttcatt ttttctatta ctttagataa agctacagaa
      961 atttttatac cttcacaacg cgcatcttgg atttcaataa tagtatcatc aattagtttt
     1021 tgaatttctt gcctatcaac atattcaatc ttagaaagag aatcttttaa agctactaat
     1081 gattcaacga tttctttttt ctcttcagac attttcttcc tccttctagt gacccgtaaa
     1141 gccatgtaga gcagagtcca ttgaaccagt aattacacta tctacaacgc cattccaatt
     1201 tactctacat tttccatttt tcagatttgt cggtccatac ggatagcctt tttgacattc
     1261 aatgttcgga ttataccaga taggattata ccattcagcc gcagaagcag attcagtcga
     1321 tacaaacaat cccacaccaa caaatccaat acacatcaca ctatagccaa ttagtttttt
     1381 catctcgtat ccatctccta aaaaatagaa tagttcttat agttattcat aagacatatt
     1441 ttttccgttt ttgttttatc ctttctgtcc tataacaact tttttcaagt aaagaatgac
     1501 atagtttttc ttaaaaaaac agtctgtttt tttctatttc ttcggtgtat aataaatatg
     1561 agtgttcatt ttttatttta atacgcactc cgtacataat aaatcttgat cgcatcaatc
     1621 ttttgagctt cttgatctac tttcaattga agtaatacaa attgagtaac cttattcatt
     1681 gtttcttctc caaataacaa atattgatcc ggattctcta tatcttgtgt cacctggtaa
     1741 agtttcccag ttgttttcat ttgtacatgc gggtctacat caatactatt gtctttaata
     1801 caacgatctg tcatatagtc cctcacagct tgattgcgtt tataaacatc tgtgaaatta
     1861 ctccattttc tagcaaaatc ttttaagaaa ttttttgtca tttcatctat ttgcatttgt
     1921 tgcgctgtct cgtcttctaa tgaaccaatt tgactgttac tttcagtact ttgaagagtt
     1981 ccacttgttt cttttactgc ttcgattgtt tcttctgtcg ttctcaattg tgttttttgt
     2041 ttttcagaag aaaaccaatg acaaccagta agacctatca aacataacca actaaatact
     2101 aaaattatcc cttttttcac gtttctagtc actgcctttc ttatttcacc ttacgaacca
     2161 gactacgaat ttttgtttga ttgtacgtgc gttgataccg agccataatc cgtccttgct
     2221 cggcattctg ttccacacta gagaaatgtt gtccgccata tgaaacatct gttataattc
     2281 cggtgtgacc atagattcca ggagacaatg caccacctgc ttgccaattc accacatctc
     2341 ctgcttgtaa atcacttggt tttgggttca tgattacttg ccaaccatag gttgtccagt
     2401 tataatcttc ccctattaac atcgcaaaca tgtgtccaga acccattaac tgtgggccac
     2461 ctaattgaga tacataccat gccgtaccac cataacattc cccattattt acggatcgtc
     2521 caatgatccg atccaaaacc ttcgcattgc ctttactttt tcctgcaata agcgaagttt
     2581 tttttgttgg taggctattc caatgcttga tcgcaaaagt atcatattcc gttaagttat
     2641 aggacgttat gatactcatt gctcctcgat aatacggact cccttcggta atacctgctg
     2701 ccgcatagcc tccacgacct agaatcaata aacaacttcc aggatcttta ttgcccacac
     2761 attgattaaa gttgctttgg ctcgccacca taaagccata atagtcataa caagctttta
     2821 agttcggaaa cttcatatag aaaccacctt cagaaccacc aattccacgt gcagaacctt
     2881 gtgggtaggg acaaccagga aaccaggtaa tcccaaaaaa gttcgtatcg tttcttccag
     2941 attgtgactg tccataagct gattcatagc ctaattgaga aagaatcaca ctcggtaata
     3001 actgatactt tacagctgca ccaactgctg cttgcgcatg ttcatctgtg attggaccac
     3061 gtcctttaat ctctgaaaaa atcggtaatc cttcggtata ttccccatta aaagcgacag
     3121 ctagacctgt agcttctcct ccatgctcag aaagttctcc tgaaaaaaag aatggcaata
     3181 gaaaaaaggg aagaataaag aaaagaagac aacctagaca ccctttcttc atttcttcct
     3241 catcctttct tcgataatgg tttttggact gttcgtcctt ttggtaaagt aaaatcagtt
     3301 cctggttttg cgtccaaaat cggcagtgtt ttaggatcct tttctatttt ttccgattgc
     3361 tctgttggct tatctaaaga agaaaatgca ggttgtaacg attttggtgc ggaaatcgac
     3421 ggtttacttt cagacgaacg ttgccccggt tgttgcacag aacgatttcc tgatagagtg
     3481 gctaccgacg aattaggaac agagtattgg ttcaataact gctgcattgc tggataactc
     3541 ccatttttcg cctgattttt cacttggcga atctgttgtg aaagagaacc agcagctgtt
     3601 aattgtggca cgggtacttt tccagaaggc aacggaatcg attgaccaac aaaagcgata
     3661 cctttattta acaatggttt tctttgatta ttcccttgtt gcttactcaa ttgtgccaat
     3721 gattgtacct gttgttttgg tcttactgaa ttcatggacg aatttggaat caaaccattt
     3781 gaaagaccag aattccactc tgttttcgat ttggtagaag acatttcagg ctctacccca
     3841 tgataacgaa catgacgacc actataaggc aaggcttgat ttgaacgccc acctagttca
     3901 aagggattaa ccacttgcct cccttttacc gattcttgtg gtgtacgttt ttgttgaaac
     3961 ttttgaaatc catgtatcgc tgcagaagcg agtggattcg ttgctcgata cacattccct
     4021 gctaatcctg cacctttgat tgccaaattt tgtccagcag ttgatagttg accaggcagt
     4081 tgtttcattt gagacaaaga atgacttgct atgtttttcg tgttgtttat agtttgatct
     4141 gcagctccta accctagttt ctttaataaa tctttccgtt ttacaatgag taaaattgat
     4201 aggagtgctt tgacaaacat atttcctaaa aaaccaaaaa cagtattcat tggaattaaa
     4261 tcgtctacta tgttatagat caaaaatgcc accaaaataa tcacactgta aaaagatttt
     4321 tgaaacatca agcccaaaaa ggactttaag gtattcatga gcatataatc aaatactgga
     4381 acaaaagaag cgagtaatga aaaaggaatc acaagtaata aacacaacac caacatttga
     4441 aagataaaac gaatcatgcc aatcactaca agtggtaatc caatacttag attaagcaat
     4501 ggcaccatag aaccaacgac tgctttataa acagctttag ttggttgaat atagacacga
     4561 taagccttct ttttccctct atcttctcct gcagcagtgg tcacttttgt ctcaatatca
     4621 gataattctt cacttgacgc ttccttggct agaaattctt gtggttcaat ctctgcttga
     4681 tacaattctt ctaaatttgt tgtcccataa tttaacaata tatacggatc aacaacagcc
     4741 gctttataat aaagttggcg cactttttga atggcttcct tttgtgtcgt tggcactaaa
     4801 acaccattga catcttttgt cgccaattca tctagactat ttgtagcaga aaaaataaag
     4861 ccttctacct ccacggacaa atcatttaac cctttggtaa aagcttctcc tttactaggt
     4921 acgttactac catagccaaa ccaaaccatt gaaaaaatga caacgaagag aaatcgaata
     4981 aacaccattt ttgcgtaatg tttccctcta aatatacgaa gagtaaatag atataacaca
     5041 tacaagacga caatccccgc accaatagga tcgtatattt ttgaataaat agtatccgaa
     5101 aaagtgaaaa actggtgaat taactcgttc attgcagctc cttcatagag cttctcaatg
     5161 acaaaatcaa ggccttgcca aatcaattta cttaaactaa agaacatatt cgtcatcatt
     5221 tttagaaaat ctgtgccatt tgccccaaat aacattttgc ctaatgaaaa ttcactatca
     5281 gataaataag aagaaaaggt atcaatatga aatttcccaa tctcatagat cgattgatcc
     5341 acctgctttt ctgccgcata caccaatcca ccaacaaaaa gcagtccgaa tccaatcgtt
     5401 aaaaaaaaca aaacgcacca tttcttttcc ttatgcttta tataagccat tgtttcacct
     5461 catttctatt gcatgattaa cgtatcctgt tcttctactt gagagtgact tgttttttcc
     5521 gttgtttgta atgcagcggt catttcttca aacaaactgt gtacattgag ttttcctaca
     5581 cgtccatagg gatctaacat ccaacactgt cctttaatca tgtggctcat ttcagctaag
     5641 ttcgcttcat ttacttccaa acgtaaatga ttcaaaatct tttctcgctc actgtcttca
     5701 tcaaaagcaa agacgacacc aaaattccct ttttctttat ctgaacctaa gtcaccaata
     5761 aattgtgtat ctaacactaa ttgattcatc tgggaacgcc ccacacgtgc cattgctgca
     5821 ataatactac gtcctgcgtt cgaattatta aatacccatg cttctgtaaa ataaatcgct
     5881 gttttcttac tactatctcg ttttccaaac atttcacaaa aacgacctaa actaatcatc
     5941 aaacacaaag actttcgatc cgcatctgta tatagttctg gtcggacatt gtcatttggt
     6001 aatttcaaat cttggatctc taaaatcgtt gttttggcat taaagtctaa tcccgggttt
     6061 tctccataag aaaatcctaa acgtaaaatc gaatcttcta ccgtcaagcg taaaaactca
     6121 ccatattctc gaacctcttt ttgttccatt tgttctaaat agtcgaccac atgcatcaag
     6181 ccaacttgtt gtccggcttc tttttgaact aaaacttctt ggattccctt taataccgcc
     6241 ccttttaaaa ggtgattggt acctaacgga cttaactcat caatcatatc ctgggctact
     6301 tgtttcgcat ctactcggga taaacaaaca attggatcta atacaccata attacgggga
     6361 tttgtagcat ctaacgtgac ataactcatt ttttgcaaat gttctacaaa caaagggtaa
     6421 tgtttctgat aatacgggtc ttgaatgagt ttcgtaaacc atttgcgctt ttctcctttg
     6481 ggatccacaa ataaactttg tacattcata aacgaactat acaaaaagag caagccaact
     6541 aaaaaggtct tccctttacc ggtttttcca gttactgcta catgaggcgc atcaaacaac
     6601 gcacctttga ttccttccgc aatggctaga agattcaaaa agacaaaacg atttgacgca
     6661 taaattaatt cttctaatgg tgttttttct cctcgagaac caccatcccc taatgtatct
     6721 tgccgaccaa tataccagcc ggattccata cctaattctg atactgctga aaacaaccat
     6781 tcgccaactg cttctacagt tgttcggtgc ttccaatgta actgccgtcc caatggttgc
     6841 cctaataaac atttatagaa caattctcgt tgatcatttt ttgcacggtg gatatggata
     6901 atcgtatgaa gttgttgaat aaccgttgca ctattttgac gaacttcttc aaccgtttga
     6961 ccgtatacaa taaaacatgc gatccaatcg acgacaggtt gcccattatc tactcgttgc
     7021 tttaaatgtt ttactaatcc tcgaacagtt aataagcgtt cactaggttc ttcatcattt
     7081 tgaaccaatt ccttatgctc tgttttgaac cgttccgata gccaattcag ttttaaacca
     7141 atacctccct gtcctttcaa gcgtggatag tgaattttca ttcgcaattc tgctgggaaa
     7201 gaaagatcct gagcaaattg aaatagatca caatttctca tattttcagg tgtttcccca
     7261 ataggcaaca atgctaaata actactccct aactcatctt ctaattgaag aaggccttta
     7321 tgttccttcg gtatacgtac atacgttcct tgattgtctt tcagtacttc ccaattattt
     7381 tgttcttcta ctttgtgagc cattcctcga atatattgca gacgattgag atacaacagc
     7441 tcttttttta ctaagggacg aatagaaaag ctataaagta attgtgtcac ttcttttaca
     7501 taaggatcac tttcagaaaa caaagtttcc aaatcatcta cttctaatcc aaataagcgt
     7561 gctaaacttc ctgctaaatc taaccagatt agatttatta catcatgaaa actctccacc
     7621 gttgcttttt ctttgacttt gattcctaaa ataaatgaag gacgatagac ttgaccttct
     7681 cgttccaaca atgtcactgt ttcttttgca taatacgcac caattgcttg actatcggga
     7741 gaaaatccct ttcctaatgt gtcaaacttc gtccgtaaat tcattgttcg tggatacatc
     7801 accaactcca catcttcata atctgctaaa agcaacagta aacgagccat catttctttt
     7861 atttcctgtg tcttttttgg tgtgtttttt gaaattgttc gtggaaaaac atgatagtag
     7921 gcccagatac aatcctctcc atttaacaaa caatttttct tgaatgcttt tactggttct
     7981 tttacaagca ccaatgaatt tcacctccca ataaaaaagg aacaaccagt tgattgtccc
     8041 tttttctaat cctctaaata aacatactta ttcacaaaaa gtaattcttc caatgcattg
     8101 tttgatacct tgtcccagtt ataatacaca caatcaccat tcatcatacg aatcatataa
     8161 caaaa
//
LOCUS       DAYYHB010000104         7902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000104 DAYYHB010000000
VERSION     DAYYHB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.106"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..559)
                     /locus_tag="ACHA6B_002355"
     CDS             complement(134..559)
                     /locus_tag="ACHA6B_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949900.1"
                     /translation="MGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMASIVGIG
                     FAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIYLLHILV
                     FSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(665..1075)
                     /locus_tag="ACHA6B_002356"
     CDS             complement(665..1075)
                     /locus_tag="ACHA6B_002356"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8949901.1"
                     /translation="MISFEKAFSIFAIVLMYYFQQSYLPAILNKAISIGDTGVHIFFF
                     CSGFGLYLSYLKKPITLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSH
                     VLLFKIFLPVYEESLGPFGLFRQLFSSISYSYQL"
     gene            complement(1374..3026)
                     /locus_tag="ACHA6B_002357"
     CDS             complement(1374..3026)
                     /locus_tag="ACHA6B_002357"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8949902.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3086..3226)
                     /locus_tag="ACHA6B_002358"
     CDS             complement(3086..3226)
                     /locus_tag="ACHA6B_002358"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8949903.1"
                     /translation="MIKFVFLCSCLIEFLQLFLHIETFQLSDLFYNTLGGGISGVIYY
                     LS"
     gene            complement(3220..3576)
                     /locus_tag="ACHA6B_002359"
     CDS             complement(3220..3576)
                     /locus_tag="ACHA6B_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949904.1"
                     /translation="MFLYLFAMAEEKSGLGLKKSFLVWIRYFRKSSSFRKMFLLVFFT
                     NMILFRTLMDRYVWLNPLSNVFGSWWIYSVNETTGEIRLTTECFENVILFIPFIILVM
                     WYKASEKDFGKIYQRL"
     gene            complement(4038..4748)
                     /locus_tag="ACHA6B_002360"
     CDS             complement(4038..4748)
                     /locus_tag="ACHA6B_002360"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8949905.1"
                     /translation="MGIVAGLSTVAVPTFFIISSFLLCSGEVTSAKLSKQIYRVLKIY
                     IIWSILYIPLVLKNWVQSDFSWKTDVLLYIRDFLFIGSYYHLWFLPALAFALWLVRKS
                     QVSKIKFLSGLLLCVVGLAFESYWFILSGNDSIEYIRYCYKSVFVTTRNGLFIGVPCV
                     IIGQHLAFHKNTILNKVFKYRNFILCAVLAIGCLLVGESFIINQIALREVNNLLIFSI
                     PLALVVVSVSLSLKIVHL"
     gene            complement(4897..5613)
                     /locus_tag="ACHA6B_002361"
     CDS             complement(4897..5613)
                     /locus_tag="ACHA6B_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8949906.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5638..6036)
                     /locus_tag="ACHA6B_002362"
     CDS             complement(5638..6036)
                     /locus_tag="ACHA6B_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFM8949907.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6124..7764)
                     /locus_tag="ACHA6B_002363"
     CDS             complement(6124..7764)
                     /locus_tag="ACHA6B_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFM8949908.1"
                     /translation="MRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDV
                     CSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINF
                     ADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLF
                     SGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHAD
                     KYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIF
                     EYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDN
                     IQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRE
                     LFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVD
                     GKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIE
                     ALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2653 a         1675 c         1153 g         2421 t
ORIGIN      
        1 taatcattta gttttatctc ctttttacca accatctacc actaggttac gaactaccta
       61 agtgcccacc atccgccgcg tggaggctgt taatcaggat ggcaaccgcc cggtagaggc
      121 gacaattatg ctatcatttt aatttaagtc tatataacat aatactataa taatatgaca
      181 acaccaacgt tataggaatg gaaacaaatg ccgccaaaac caagttaggg ataatttgat
      241 gaatcgtact aaatacaaga atatggagta gatatatatc atatgagtat cttgaaatcc
      301 taggcattat ctttctaaat ggaataactt gatacattaa aattgcaatt gcaccgtaac
      361 cgaagaatgc aaaaatatca ttaatagact ccatcagtcc accaataatt ttcgatatac
      421 cagcaaaacc aattccaaca atggacgcca taatcacgaa tatattatta aatcttacta
      481 tttttctttt cactaaatac tctgcaacca aacatgccga aagtaaattc ccaaatatat
      541 tgcgcaaagc agctcccaag aactcgtatg cttccatatc ctgtatgaaa catcataata
      601 gcccagatac taataactca gcttgcaaca agcaatcatt tcattcctat ttttctctat
      661 agttttacaa ttggtatgaa taagaaatag aactgaataa ttgtcgaaat aaaccaaagg
      721 gacccagtga ctcctcatat actggtagaa aaattttaaa taacaaaaca tgagacaaat
      781 atacttttgc agcatttaaa tcttgtgtca tataaggtag taacactgat accgtaatta
      841 cgataatata cggaatataa atcttgataa atcgtttctg taaaaattca cccaatgtaa
      901 taggcttttt taaatatgac agatatagcc caaaacctga gcaaaagaag aatatatgca
      961 ctcctgtatc accaatagat attgctttgt tcagtattgc tggcaaatac gattgctgaa
     1021 aatagtacat caatacaata gcgaatattg aaaacgcctt ctcaaaacta ataatatcac
     1081 gtttatttct ttccccctca ttttattatt tcaatagttt tattcatatt ctactccttt
     1141 catctctatt gctgaataca ttattaaaac tctaatatct cttatttctc ccttactgct
     1201 ttgatcggtg cttcatcagg ctgctgtgtc tctgttaaca cttctttgcc ttttcgatcc
     1261 acacagtcgg cccctttacc tcaattatgt aaacagaacc gtaaagcagg aaatttttat
     1321 taaacataga tgttgctact tcggacattg acactcagat ctttttcata gtattactta
     1381 aacatttcag ccagttcagc agccataaca gcttccatta cttcggcagc ttccttgctt
     1441 tctgcgctaa cgctgatata ggtcttcagc ttcggctcag tgccggaagg ccgaaccact
     1501 acagagcagt tgccctccag cagatatttg agaacatcag acttcggcag gccattcagt
     1561 cccgtgctgt aatccaaggt ttcaatgacc ttcttacctc caatcatatc aagaccttta
     1621 tggaattcaa ccataatttt ctgcatcttg gtaaaacctg ccgcaccctc aaactcaaag
     1681 gaatgcaggg tgttcaaaca gtagccatac ttagcataaa tctcgttcag cttatcaaac
     1741 agactgatac cctgagtctt gtagtaagag aacatctcgc agatcagata agcggcgttg
     1801 acaccatcct tgtcacggac atagctgccg gatagatagc cgtaggattc ctcaaagccg
     1861 aaaatatagc tgtccacctt gcctgccttt tccagcatac caatctgttc gccgatgaac
     1921 ttgaagccgg tcaacacatc gacagtacgc acgccatagt tggaagcgat ccgctcgccc
     1981 agatccatag tcacgatggt cttgaccatc acagggtcag tgggcatctt gccgtgctta
     2041 attctctgag agcatatgta gtccagaagc aacagaccag tttcgttacc ggacagcagg
     2101 acataatccc cttctgcatt cttcacggcg atacccacac ggtcgcagtc agggtcggta
     2161 gccagcagca gatcagcatt atacttcttg gcatactcca tgcccagaga catagcttca
     2221 ttaatttccg gattcggata aggacaggtc gggaaattac cgtctggcag acgctgctct
     2281 tcgacaacgg tcacattgct gtagcccatt tctgccagag tacgggtaac aggcaccaga
     2341 ccggtaccat tcaaaggaga atacacgata gcaacattct tgtccacttc ttcgccaaac
     2401 agaaccgact ggccctttac ttcttcaatg aaagcagtca gaacctctgg ctggatagaa
     2461 gcgattctgg aagcgtcgcc gcccattttc acatctgcaa aaatatccag cttttcaatt
     2521 tcagccagaa tctcagcggc agcctcggtg gtgatctggc agccgcctgc gccatagacc
     2581 ttatatccgt tatacttgga aggattatgg gatgcggtga tcataacacc ggcagaagcc
     2641 ttcagataac ggatagcaaa ggatacggta ggcacaggca gcagctccgg ccacagatgc
     2701 acctgaatac cgtttgcagc aaagatgtcc gcggcaacct ttgcaaatac atcggacttg
     2761 attcgggaat catagcccac gacgacagaa ggggtttcat aggtcttatt cagatagtct
     2821 gacagtccct ggcttgcctt acctacggtg tgtacgttca tacgattagt gcctgcacca
     2881 atggtgccac gcagaccacc ggtaccaaag gacagatttt tatagaagga gtcctcaatg
     2941 tccttctcgg aatatgaccg cagttcctct acaatttctg cattggcatt tgtaagccaa
     3001 tgataataat ttgcattatg atccatagaa tctctccaat cttttaaaat tctgctcatt
     3061 tatttttaag gttaggatga atatatcagc tcaaataata tataacccca ctaatgccgc
     3121 cacccaaagt attataaaat aaatctgaca attggaatgt ctctatgtgt aaaaataact
     3181 gcaaaaactc tataagacac gaacataaaa aaacaaattt tataatctct gataaatttt
     3241 tccaaaatct ttttctgaag ccttatacca cattactaga attataaatg gaataaacag
     3301 aataacattt tcaaaacatt ctgtagttaa tctaatctcc cctgtcgttt cattcacaga
     3361 gtaaatccac caactaccaa acacattaga caaaggattt agccaaacat atctatccat
     3421 cagtgttcta aaaagaatca tatttgtaaa aaacacaagc aaaaacattt ttctaaatga
     3481 gcttgatttt ctgaaatacc gaatccagac aagaaaagat tttttcaaac ctaatcctga
     3541 tttctcctca gccatcgcaa acaagtataa aaacatagcc aaaactgcaa ttaaaatcga
     3601 aacccaaata tttgatacaa tgcagataac acattaacca aaatagaaga tatgattttc
     3661 ttcataaact ctcctttcat catgcgctaa atgagtacca tcaagaaggt ttcgtaaaag
     3721 gaggtaccca ctctaacatg aataacctcg gccagcgaaa ataaaactca acagaattcc
     3781 tatctctatg ctgctataat ttcaacaaat ttacatgtac cagttactaa tctaatataa
     3841 ttttttcagc aaggtaaatt tcacactaca cgcatacaca aaaactgtag aaattaaaat
     3901 tattatcatt ccacaagtca tttctattaa tgaatttgta atccccaatt ttgggaaaat
     3961 aaaatttaaa gcagctatta tatacggatg taaacaaaat aataacgtac taattcttcg
     4021 cagatgtact gttattttta taaatgtact atctttaggc tcagtgatac tgatacaact
     4081 accagtgcta atggaatgga aaatattaac aaattattga cttctcgcaa agctatttga
     4141 tttatgatga aactttcacc aactaataag catcctatag ctaaaacagc acataagata
     4201 aaattacggt atttaaagac cttattaagt atagtgtttt tgtggaaggc taagtgttgc
     4261 ccaataatga cgcatggtac acctataaat aatccattac gagttgttac aaacacgctt
     4321 ttataacaat atctaatata ctcgatggaa tcattcccag ataatataaa ccaataagat
     4381 tcaaatgcta aacctactac gcatagcaac aaaccactga gaaactttat tttagaaacc
     4441 tggcttttgc gtactaacca tagagcaaaa gccagtgcag gcaagaacca caggtgatag
     4501 taactaccaa taaacagaaa atctctgata tacaataata catctgtttt ccagctaaaa
     4561 tctgactgta cccaattctt taatactaat ggaatatata atatagacca aataatgtaa
     4621 attttaagta ctctgtagat ttgtttagat aatttagcgg atgtaacttc accactacac
     4681 aacaaaaagg aactgatgat gaaaaaagta ggaacggcaa ccgtactcag tcctgcaaca
     4741 ataccaatac taaatgctta atctggagct aaatagtttt gacctaaatg atgaacaaca
     4801 actaaaatag ccattagtat ttttagaata tctatcgaat agtactcccc cacacacgca
     4861 gctttataat taacattttc tatgattata ttttccctat tcatttaaga acacatcgag
     4921 ctcttctgga gtaccgagta cgtgaacttt tgatttatct actatactaa tggttacctt
     4981 catgcccttc tctatcatat aattatatag cggcgcaata tatttctcat tcttttccag
     5041 atgcctttca tctgcataat attcttcgta gagtgatgca tataattttg cagaagaaaa
     5101 ataatatgca cctaaagtac aattgttaga aattctaact ttttctctta cttctactac
     5161 atcaccatat tcgttcaact tggcaaaact ccaatgatca ccgtccgcat ggaaacacgg
     5221 aatatgtcct tctccagaaa tatcaacgta tttcatctca cctggctcaa catatgtatc
     5281 tatgttataa accataattg cattatcagg tgcgcaataa ggaattgcaa gcatacatgt
     5341 agtggcttgt ccatcagtca tatagtcaat ttcaactacc tcgacatttt caattccata
     5401 cagactgcaa tgttctttga taaaaacagt agcatcatct tccttacgca caacaaaaac
     5461 atacttttct acatgtgaat tataatcaag caagctgtct aaggaccaat caaataaggt
     5521 tttacctttt gcttcaatca taaatttggg cacagtgtag ccaactttac gaaagcgact
     5581 gccaagacca gccattgtaa taataaccgt catcttattc actttataaa gtctccttta
     5641 ctcagatttc aatttggatt ttttgcaaat ctcatcgagc tgatccaggg tacacgataa
     5701 aaattcatcc ggacgaactg tgcgatcatc aacataaaac cctttatggc ctggccaagg
     5761 ctttccataa agaatttcat catacggaat ctcccactta tctagccaag ccaacaagat
     5821 ttttgcagta tttgcattga tcacacctaa attaccatga taagaattca tattgcgaga
     5881 tgtgaacagt gtaatctttg ctccgttctc cttatagtat cgcaacttct ccaccatatt
     5941 agcatacgga actaaatctt catatttctc atctttcttt ttgatggggc acaaagttcc
     6001 atcaatatca aaaacaaatg cgtaatcttt aaacatataa ctaagacatc tcctttattt
     6061 tagccaggca atttatatgc ccggctttac actgatcaaa tagaaaaaca atagctcaaa
     6121 gcattaacaa taaatatccg ggacaactcg tcctaaaatt tccaaaccag ttgctaacat
     6181 agccatctga tggtttaagc tttctccatg caaaggaatc atacttaaaa acagcaaagc
     6241 ctcaataagt tcaatttttt tcaaatcaga acctatttca tctttgaaga cagagtaaaa
     6301 tacttcatat aaatcataat ctcgtttacg atcttgaaca atatagtcta tgattgcctt
     6361 ctttggatca tattttattg taaacaagtc cttaattata aagtcatatt ttccatcaac
     6421 actatgaaac aattttgcca gttcatatct ataatcacca taaatatcaa aatctccaaa
     6481 cttacctcgc ggatctatga ctttaatgaa cgaaaatgtg ttatctacca taatattggc
     6541 aaagcataag tcaccatgaa taatattaaa ttgcgtaatg tcaaacaatt ctcttggaac
     6601 acttacgctc aataaagctt ctatctggtc caaagattta tatctgactc catttatttg
     6661 aatatcagaa gaaaagaatt ctgtgaaccg ggggtccttt ctgagcttat tgaatctctg
     6721 aaatgtttta tcgagataca tatctttcaa tgacttttga atattatccc ctgaaacaga
     6781 gtatctctta aagtctgagc atacaaatcg tattctatta aagatatcta tccattgttt
     6841 ttttgttaag tcactataga gaaataactc gtgaacagta tgatacgaat agtattccat
     6901 acttacatat ggattaatat acgaagtaga atattcgaat attctaggtc tcacatattc
     6961 tacctgtgct ggcaacttaa gataccactt aatttctccg ataaattttt caacatcttc
     7021 actaattttt ctcaggatgc tacgatcttt atcaattgaa atgtggttaa attctcttgc
     7081 cttaacttcc aatttggaat tataatattt atctgcatgt ccaatgtcaa accaattatt
     7141 tgttttaata gagagaaatt catatcgttt gctatactct tgtaaagctt gataaaaact
     7201 attcacattt actacatttg acatcaaagc tttacgcaaa cactctctaa aatactgggc
     7261 atccataatt tgaaatacac cagaaaacag tttgtgcttc tttccatcat cttcttttaa
     7321 ctcgttctta tctaaaacag aaataatatc tccgtctttt tcttcaaaga atgtccaagt
     7381 attactataa tagtcttcag catagaaaaa gctgtcacat tccaaggaat atatattatc
     7441 attgataatg gtatcagcaa aattaataat aacaggctcg tcacatccta ttagagaatc
     7501 atatatagtt cttccaagat cccctaactc tttaagctga ataatattta ctgttttaga
     7561 tttaatatac ttatctaatc gtcttgctac cttatccatc ttttcgtaac aggcaatatc
     7621 gatagcagaa catacatctt tatattgatc atataaataa tcaaatacaa ttttttgatt
     7681 aatcggatag ataattgcag gaagttttcc aagatggtga agttcttccg gtacaatctt
     7741 agcagatgga atcaataatc tcatttcacc gtgcctccgt tccattccca agatttgctt
     7801 gttaattttt tatattttta aggattttca ttatttgcgt ccttttaaga aaagccacaa
     7861 taactaatcc aataataata ctatatctta gaattgtaga aa
//
LOCUS       DAYYHB010000105         7895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000105 DAYYHB010000000
VERSION     DAYYHB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.107"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHA6B_002364"
     CDS             690..1658
                     /locus_tag="ACHA6B_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8949909.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACHA6B_002365"
     CDS             1726..2484
                     /locus_tag="ACHA6B_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8949910.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHA6B_002366"
     CDS             2552..3262
                     /locus_tag="ACHA6B_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8949911.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3322..4539
                     /locus_tag="ACHA6B_002367"
     CDS             3322..4539
                     /locus_tag="ACHA6B_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8949912.1"
                     /translation="MIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNENTELIAYD
                     DQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKADEPIYAHGW
                     DAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKETSAESRVLL
                     IKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKILSNLYVQAV
                     QEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVASPEIDYEA
                     IKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDLEMVTQKTA
                     EKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKHNVLRCEPN
                     CHAFLSEERGQEQ"
     gene            complement(4597..5784)
                     /locus_tag="ACHA6B_002368"
     CDS             complement(4597..5784)
                     /locus_tag="ACHA6B_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8949913.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            5942..6271
                     /locus_tag="ACHA6B_002369"
                     /pseudo
     CDS             5942..6271
                     /locus_tag="ACHA6B_002369"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            6319..7719
                     /locus_tag="ACHA6B_002370"
     CDS             6319..7719
                     /locus_tag="ACHA6B_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8949914.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT         2473 a         1318 c         1610 g         2494 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaatgagtc aagtccacaa
     4441 tcttgtgtaa aagttcaacc tcatcaaaag cacaatgttt tgcgttgtga acctaactgc
     4501 catgcctttc tttctgagga gagaggtcag gagcagtagc gcagcgactc ttgatgtctc
     4561 tcctcagaaa gaagagactc gcacaggttc atggtgctaa atgttattcg aattaaattc
     4621 taaatatttt cgtggagaag ccagccactc agtgtctttt tccatcaaaa gtgttccaat
     4681 tatccgagtg atggaattga tatttggaaa aatccgaatc actcgttctc tccggcgaat
     4741 ctcttcgttc aaccgttcca acacattggt tgtacgaatc cttcggcgga tattttcagg
     4801 aaaagacaaa atttgaatag catcttcaaa gccgttttct aaaatgtcgc aagcagcact
     4861 taattttgaa tcacaaccat atttttccaa gaagtgctct tttctttcac gtgtcaattc
     4921 aagttcagag gctttaaaga tactcttcaa ctcgtctttt acatcagaag aaactttctt
     4981 tggcagctta tcaaaaatat ttctcaaaaa gtgggcttgg catctttgcc agctcgcacc
     5041 taaaaagttc ttgcgaacag ccttaactaa ccctttgtgt gcatcagaaa tgaccagttt
     5101 tacaccgaac aaacctcgtt gaacgaacga ttgaaaaacg gtatcccaat tatcttccga
     5161 ttcgccgtct tgaacatcaa aaccaagaat cgtacgtcgt ccatcagaat caatgccgat
     5221 accaatatat acaccttttg agacaatacg atgattttcc cttactttca tatacagggc
     5281 atcacacatg aaaaatggat attccgagcc ttctaggctc cggtttctcc actcaaaaac
     5341 aaatggatcc aactgtttca tcagatttga aacgaatgat ttagaaacag tcgcaccatc
     5401 cgttagcagt tcaactgttt tagttacttt gcgagtggag acaccagaaa tgaccatttc
     5461 aatcatggca gcgatcaaag ctttttcgct tcgctgatat ctttcaaaga tagtaggact
     5521 aaacttaccg tctctggttc gtggaacatg aagttctaac gtaccaattc gagttgtaaa
     5581 cgaacgatcg tagtaaccat ttctttgtga ttgccgttct tctgaacgtt cataggcatt
     5641 tgcctgaata tattgatttc gttgttcttc catcagctga ttaaacatga tagtcataag
     5701 tgtttttgcg gtatcattcg caccgctttc gtttataatt gcttgaattt cttcgctttc
     5761 aaaagtaaaa tgtacttgag tcattgatat cagtcctatt cgagtatgtt ttctgcaaaa
     5821 aacattgtac tcctaactga gatcaatggc ttttttgatg cttcactttt acacaattat
     5881 atggacgtaa tcaaaaaatg tttccacaga ggacttcttt aattattgtc gattagagga
     5941 aagtggtgta acagtcacta gcggtacaga tttaccgctg tttattactg atattcctga
     6001 atcgattata agagcagtat tccgtcgttt tcctgaagga gaagaagtct gggagaaaga
     6061 aacaagcttc acgacaaccg agctgctgaa aagttttaca agcaacagct tcaaagtaaa
     6121 tggtttatcg gagtacggag aaataaaagt agggaaaagc gccagcttag ctgttttcag
     6181 cgaggacctc tgtactgatg actggcaaaa agtgatggct gcggaagtta tcgctaccta
     6241 tcttgatggt gacttagttt acaagaaata atgcttttcg atataacgtt ataaaacgaa
     6301 ttaaagagag gttaaaaaat ggaaacaatg aaaagaaaat tcaccttccc acatagttat
     6361 acattgattt ttatcttaat tattttggct gcaattttaa cttggataat tcctagtggt
     6421 cagtttgaga cagttgttga aaacgtcgca ggtatggaaa aaacaacggt tgttccggga
     6481 acctatcacc aaatcgataa ggcagattat ggacaaggtt tttcctctat actaatggca
     6541 ccagctgaag ggattatttc agctgttgag gttgttgctt ttgttctcat cgttggtggt
     6601 gccttcggga ttatcctaaa aacgggagcc atcgatcgag ggctgtttac cttagcaaaa
     6661 gttctgggag acaaggggat cttagttatt ccgatgtcga tgattctatt cagcttagga
     6721 ggatcaacct ttggcatgag cgaggaagtc attcccttat ttgcggtgtt tatttctctg
     6781 atgttttctc ttggctatga ttccatgact gcgatcttga ttctattttt aagtacacaa
     6841 gcgggatatg ttgggtcgac gattaatccc ttcaacgttt taatcgcaca gggagtagct
     6901 ggcgtttacg gaaatcccct gctttggttt cggatgctat cctggattgt cattactggg
     6961 atttcaattg ttttcaccat gctttatgct agcagagtca aaaagaatcc actgtcctct
     7021 attgtttatg agagtgattt aaaggttaag cataagtttc aaaattttga cactgaaaaa
     7081 gagttttcaa cacaagataa actaattatc gccggtttta tcgtaggttt aggaatcatg
     7141 atttggggta tcatttccaa gggatggtat atggttgaaa tttctgcact attcttagga
     7201 ttaggtcttt ttgctggggg catcgctaga ttttctcaaa aggaaattgc tgaagctttt
     7261 gtcgaaggct gttcagattt cgcctatgct gctgtaatca ttgggctagc tagaggagtc
     7321 ttggtaatct tggaaggtgg aatgattatt gacactattc taaacagctt ggctaacctt
     7381 ttaggtggtc ttccaaaatt cctgttcact acgattctac tcatttctca agtaattgtt
     7441 tccttttttg ttccatcctc ctcaggagct gcggcattaa cgatgccggt tatggcacca
     7501 ttgggtgatc taatgaatgt aagtcgtgat gttgttgttc taagtaacca gtttggaaat
     7561 ggtttaatga atattatctc cccaactagt ggtgtattac tggcaggatt agccatcgca
     7621 gagattaatt tctctaaatg gtttaaggtt ggcttcaaaa tatttgccat cttaaccctt
     7681 gtttctgctg ttttgttata cattgctaca ctcttttagt cttaatcatc cattaatagg
     7741 aggcaatagt tatgattaaa ataaatcaat tagcaggaat gaatatccac tatttatttt
     7801 attctctcaa agaatttttc gcttatcaac agaaaattgg tatttcttct gttgaacttt
     7861 ggggaggtgc tccgcatttt ttcatagatg ctttt
//
LOCUS       DAYYHB010000106         7675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000106 DAYYHB010000000
VERSION     DAYYHB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.108"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1555
                     /locus_tag="ACHA6B_002371"
     CDS             128..1555
                     /locus_tag="ACHA6B_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949915.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1647..2012)
                     /locus_tag="ACHA6B_002372"
     CDS             complement(1647..2012)
                     /locus_tag="ACHA6B_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8949916.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2009..2374)
                     /locus_tag="ACHA6B_002373"
     CDS             complement(2009..2374)
                     /locus_tag="ACHA6B_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8949917.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2566..2814)
                     /locus_tag="ACHA6B_002374"
     CDS             complement(2566..2814)
                     /locus_tag="ACHA6B_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949918.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2813..2941
                     /locus_tag="ACHA6B_002375"
     CDS             2813..2941
                     /locus_tag="ACHA6B_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8949919.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2998..4692
                     /locus_tag="ACHA6B_002376"
     CDS             2998..4692
                     /locus_tag="ACHA6B_002376"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8949920.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4765..4965
                     /locus_tag="ACHA6B_002377"
     CDS             4765..4965
                     /locus_tag="ACHA6B_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949921.1"
                     /translation="MIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTW
                     LDLLPQMQAFEHRLPRGYEKGV"
     gene            5343..6476
                     /locus_tag="ACHA6B_002378"
     CDS             5343..6476
                     /locus_tag="ACHA6B_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8949922.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6507..6827
                     /locus_tag="ACHA6B_002379"
     CDS             6507..6827
                     /locus_tag="ACHA6B_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8949923.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6856..7320)
                     /locus_tag="ACHA6B_002380"
     CDS             complement(6856..7320)
                     /locus_tag="ACHA6B_002380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8949924.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7452..>7675)
                     /locus_tag="ACHA6B_002381"
     CDS             complement(7452..>7675)
                     /locus_tag="ACHA6B_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8949925.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2516 a         1476 c         1377 g         2306 t
ORIGIN      
        1 ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat
       61 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
      121 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      181 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      241 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      301 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      361 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      421 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      481 agcttattca ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga
      541 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      601 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      661 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      721 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      781 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      841 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      901 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      961 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
     1021 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1081 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1141 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1201 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1261 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1321 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1381 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1441 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1501 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1561 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1621 tcttgataca cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct
     1681 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1741 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1801 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg
     1861 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1921 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1981 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     2041 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2101 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2161 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2221 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2281 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2341 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2401 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2461 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2521 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2581 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2641 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2701 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2761 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2821 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2881 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2941 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     3001 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3061 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3121 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3181 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3241 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3301 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3361 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3421 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3481 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3541 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3601 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3661 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3721 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3781 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3841 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3901 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3961 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     4021 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4081 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4141 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4201 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4261 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4321 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4381 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4441 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4501 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4561 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4621 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4681 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4741 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4801 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4861 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4921 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4981 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     5041 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5101 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5161 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5221 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5281 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5341 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5401 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5461 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5521 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5581 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5641 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5701 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5761 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5821 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5881 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5941 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     6001 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6061 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6121 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6181 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6241 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6301 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6361 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6421 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6481 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6541 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6601 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6661 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6721 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6781 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6841 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6901 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6961 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     7021 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7081 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7141 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7201 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7261 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7321 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7381 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7441 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7501 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7561 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7621 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aacta
//
LOCUS       DAYYHB010000107         7597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000107 DAYYHB010000000
VERSION     DAYYHB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.109"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..425)
                     /locus_tag="ACHA6B_002382"
     CDS             complement(135..425)
                     /locus_tag="ACHA6B_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949926.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="ACHA6B_002383"
     CDS             complement(543..803)
                     /locus_tag="ACHA6B_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949927.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="ACHA6B_002384"
     CDS             complement(940..1353)
                     /locus_tag="ACHA6B_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8949928.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="ACHA6B_002385"
     CDS             complement(1809..2294)
                     /locus_tag="ACHA6B_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949929.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="ACHA6B_002386"
     CDS             complement(2433..2648)
                     /locus_tag="ACHA6B_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8949930.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="ACHA6B_002387"
     CDS             2872..3672
                     /locus_tag="ACHA6B_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8949931.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="ACHA6B_002388"
     CDS             3691..5559
                     /locus_tag="ACHA6B_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8949932.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="ACHA6B_002389"
     CDS             5552..6043
                     /locus_tag="ACHA6B_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8949933.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /locus_tag="ACHA6B_002390"
     CDS             6031..6585
                     /locus_tag="ACHA6B_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8949934.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="ACHA6B_002391"
     CDS             6603..>7597
                     /locus_tag="ACHA6B_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8949935.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAYYHB010000108         7579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000108 DAYYHB010000000
VERSION     DAYYHB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.110"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..679
                     /locus_tag="ACHA6B_002392"
     CDS             <1..679
                     /locus_tag="ACHA6B_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8949936.1"
                     /translation="STNKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPS
                     KIIRFGILGIALIACLVSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGH
                     ELFGVGIPNNHDARAVEYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVA
                     LAISIIDAGFRRDKSVAAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFN
                     DEFIYIE"
     gene            696..1826
                     /locus_tag="ACHA6B_002393"
     CDS             696..1826
                     /locus_tag="ACHA6B_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFM8949937.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            1852..2937
                     /locus_tag="ACHA6B_002394"
     CDS             1852..2937
                     /locus_tag="ACHA6B_002394"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFM8949938.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            2942..3775
                     /locus_tag="ACHA6B_002395"
     CDS             2942..3775
                     /locus_tag="ACHA6B_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949939.1"
                     /translation="MKWDENMEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDS
                     RAAMFFAMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLA
                     SLKNCGCNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPV
                     FFHGSLYSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGE
                     IQPKIIIFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIV
                     S"
     gene            3759..5477
                     /locus_tag="ACHA6B_002396"
     CDS             3759..5477
                     /locus_tag="ACHA6B_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HFM8949940.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            5503..6339
                     /locus_tag="ACHA6B_002397"
     CDS             5503..6339
                     /locus_tag="ACHA6B_002397"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8949941.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            6344..>7579
                     /locus_tag="ACHA6B_002398"
     CDS             6344..>7579
                     /locus_tag="ACHA6B_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8949942.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILR"
BASE COUNT         2497 a         1011 c         1509 g         2562 t
ORIGIN      
        1 tagtacaaat aaaagtatat ggaattgcat ttttagttta ctgacatttg ttataatctc
       61 gcaaggcaga agtggtatat tatatgtagt aataacctcg gttattatgc ttttaatttc
      121 gcttaaacca tccaagatta ttagatttgg tatattagga attgccttaa ttgcatgtct
      181 agtgtctgtg acccaaatta gagaggtcgt tatatcttcg tttgtagagt ctcagagcag
      241 tgacacaggt aatatcgcat atagaattgc tgaacaagat tattttgaaa ataaattaga
      301 aggtcatgaa ctatttggag ttggtatacc aaacaatcat gatgccagag ctgtagaata
      361 ttcaggcaaa acaatagatt ttgagtctaa aaaatataat ccctattatc ttgaggactt
      421 aggcgctttt gaaattcgtt atagatttgg tataattgca tatgcgttat atatagttgc
      481 tttagctata agtattatag atgccggatt tcgaagagac aaatcggtcg ccgcatttgc
      541 gggtatggca ttcatggtgt ttatggcgct taattttaat ttgctagcgt atattacagc
      601 aagaccgttc ggatttatga tttctttaat tttgatagaa atatcggcat tcaatgatga
      661 attcatatat attgaatagg taagggagag cacagatgaa tttagatcga ttaaagaaaa
      721 tagcaaatat gatttcacca atcataaaag tgataaatgc gccgttagcg tattttagtg
      781 ggaaatcaga ttatatggag aagggaatat tgtggcctac tgtaacaacc tactccaata
      841 acagtagaaa agtgattcaa tatacaactt tacctaaaaa cgtagacaac atggctcaaa
      901 tgcaatgggc tggggaaagt tttgaggaca tatgtatagt tcttcaaggt ccagtattag
      961 aagacggtga ttttacacta aataccgtta aaatgtataa taaatactat cctaaagtaa
     1021 aacttgtagt gtctacttgg ataggattat ctgaagagac agaatgcaag ttgcgtgaag
     1081 ctggtgcaga tgttgtatta tgcaagaaac ctgaaaaaac aggtgcgtac aatattaatc
     1141 tacaacttac ttctgcatac gaaggcgtaa aaatggcgca aaaactgggg tgtagttatg
     1201 cgctgaaaac gcgaactgat caaagactat atgcaaatga tgtactaaag tatttccaaa
     1261 cattattgaa tatttttcca tcttcggact gtaaagtagt gaatcagcga ttaatattta
     1321 cgacttatgg aaaatcattt agatatcttc cgtttaattt atgtgatttt ttggtatacg
     1381 gagaaactag tgaattatta aaattatatg gtattcaaag agataaccga gcatcagact
     1441 ttgggaaaat tatttatact gaggaaaaag agtttaataa aaagatattt acactactag
     1501 aaggaaaaca aggtgtatct ccatatgatg tttttccaga ttttgatgca ctatataaaa
     1561 aatatatgtt tgcagaatac tatattgtgt ataattattt cctggaaaat attgcaagta
     1621 atgaatctta tggtgactta atggatgcct attatagtta tttaaagaaa tatgcagttg
     1681 ttgctgattc agaaaaattg ttactatatt ggccgaaata tgttcaatat aatgttaagt
     1741 tcgaaaatga acttgatttc tatgcgaagt tggattcaaa aaaatggatg gatatttatg
     1801 ctaattatac accttttagt cagtgattat atggaaagga tgaggtgaat aatgaaaatc
     1861 ggaatagtga ctattgttaa tggggcaagt tttggtaaca gattacaaaa ttatgcgact
     1921 caaaaagtgt tggaaaataa cggctataca gttgagacaa ttaataattg ttttgacaaa
     1981 gaaagtaccc tatccattgt tatcaaaaac gctatttata cattatttaa gtttataccg
     2041 catttcaaaa agtcaaaaag tatctggaaa agaatgtata cattttatca atgggacaag
     2101 cagtatatag attattctca tcgtaaaatt gatgcatcta aaaaaaacag ctttgtgagt
     2161 ataaagaatg attatgacta tttcttggtt ggtagcgatc aaatttggaa tccaaaatat
     2221 gcagccaata agggatttga acttttatca tttgttccaa atgaaaaaag agtttcatta
     2281 tcaaccagtt tgggagttcc atctatacca agtgatttag aaaaattata tgtgacagag
     2341 tggaataagt ttaaatcaat atcggtacgt gaaaatcaag cggcaaaaat aattgaggac
     2401 ttgacaggca caactgcggc tgttcacatt gacccaactt taatgttgga gcgttcggaa
     2461 tgggaagcct ttcaaaaaaa accagacaat attgatgaaa attacatctt gtcgtttttc
     2521 ttaactaatc cgcctaagga tactgttgat gtattagata aggtcgctaa agatttgaaa
     2581 ataggtgttc tcaccttaga tgcgtcttgg gaatattttc aagatattac tccgaatcat
     2641 tttgtttatt tgatttcaca tgcaagaatg atttatacag attcattcca ctgcacagtt
     2701 ttttcattgc tatttggaaa accggttgtg gtatttgacc gttttgatag tgaaaaaatg
     2761 aattctaggt taagtacatt gttaaataaa gtaaataaag cacatttttg ggtaggaaaa
     2821 aaagctgatg aagcaatgat tcgtgcaata gcatctgaac ctgaatatga catagataag
     2881 ctgatggtat cagagaggga atgtgtaaaa gcttatctta aagagtattt acagtaaaat
     2941 tttgaaatgg gatgaaaata tggagcattt aagtgattgt tttggtaaaa tgaagattgt
     3001 tacgacttca tgcgcaagag aatattttaa ggatgatttg gataaatcag agtattcatg
     3061 ttttgttgat tctcgagcag ctatgttttt cgcaatggga caagctttag ctaaaagaag
     3121 gaatgtagtt gtaatgcttg ctgatgaatt gacatcgtgt tatacggcac taacagaatg
     3181 tgcatttcaa caagttgatt taattgtgtt tgcttttcag acagtcagac atgaaattga
     3241 tttagcatct ttgaagaatt gtggttgcaa tctaattaac tgtagcgccc aagacgatat
     3301 tgatagagtt gtgtccaaca ctaaggggtt aaatcagaca acagtaatta aaatcaatat
     3361 gaattgtgag tcggataaag atcatatatg tgagtatata gacctgccag acatacctgt
     3421 ttttttccac ggtagcttat actcacctgc aagtaaaaag aaatatgagc agtatggaca
     3481 ggtatcgaag tatttaggct atctcgtggg gggaaaagat gcaatactca taatttctca
     3541 caatgagttt ttaaacgata tcagtgtatg gattaattat tattcttttg gagaaattca
     3601 acccaaaatt attatatttg acagtccgaa tgactatcaa tataaatggc tgtgtgactt
     3661 agaatgcagt gtagctcatt caaccaaccg atttttgtct gaggatttag ccttattgca
     3721 aaataacgga atttccattg tgttaagagg tgataattat gatatcgtca gttaaaaatg
     3781 ttcaaatttt agtatcttta ttaaaacagc ataatattaa agatatagtt atctcgccag
     3841 gtagtagaga ttttcaattg gtgcatagtg tagaaacaga cagttttttt agaacctata
     3901 cggttgtaga tgagcgcagt gctgcctttt ttgctaatgg actttctatg gcgattgata
     3961 agcccgtgtg tgtatgttgt acttcttcaa ctgcctcttc aaattatatg gcggcagcta
     4021 aagatgcttt tgctaatggc atccaacttg tacttcttac tgctgataga gattaccgcc
     4081 gcttatatca gatggaagat cagatgattg atcaggtaga tatgtatggg gcttatgtgc
     4141 gttctaacgt tgatattcct attgttaagg attctgatga tgaatggtat gctaccagaa
     4201 aaataaatga agcaatattg gagttaaatc atggcgttaa gggtcccgtg cagattaatt
     4261 atcaattaat ggatttaagt gcgccgtttg ttgagaccat gccagagtat cgtaaaatta
     4321 caagatatca agaggatgat ttactgtcat cagtagagat ttttaaacag aaactattat
     4381 caaaaaagag aataatggtt ttatgtggac agcaggaaga aaataattta agtgccttat
     4441 tggaagagtt ctttattaaa tataatacaa ttataatcaa ggataactat tctaatatac
     4501 acggtgaaca ctttattaag actgttctag taacagaaag aatgagtgta aatgcattta
     4561 ttgattattg cccagatttg gtaataacta ttggtaaaca tgtatggtct tttgtgaaat
     4621 atactcttag agataaaaag cttaaatttg agcattggcg gatatctgag gatggtaaga
     4681 ttgttgatcc gttcaacaag ctaacatctg tctttcagtg ctcagcggaa ttttttatgg
     4741 agactgtcac tgctggtgtg aatagcaata ataatcttga gtattataat ctatggaaga
     4801 caagattatc aaaagtttgc tatccagagt tagtctatag caatttttat gcaatttcag
     4861 aactagtaaa aaaaattcca gatagtgcat tagttcattc tagtatttta aatgcggcta
     4921 gattgtatga ctttgtatct gatgataaag cattactcag ttttgctaat ttaggggctg
     4981 atggtattga tggttgttta ccaacatttt taggtcaggc agaagcagat aaaactcgac
     5041 ctgcgtattt aatttcagga gacttgagtt tcttttatgc tatgaatagt ttatgtggtg
     5101 acattccaga taatgcacat attttcctta ttaacaatca ttctggaagt gaattccata
     5161 caaattttgg taagttttac aacggatcat taagtgttga taagcatata gctgcaggac
     5221 atatttcaaa cgcaaaggat tgggcattag aaaataatat aaaatacatt actgcggatt
     5281 ctaaggaaag tctcaaatcg gggttggatg tatttattaa tacatttgac agtcctgttt
     5341 tatttgaggt ctttacagat ccggatacgg atacgtctgt tctggaatct ttttatagta
     5401 taaataaggt aatcacacca aaaatgtatt ttagaaaggc gttagatatt ataaaaaagc
     5461 atttacacat taagtagatg tttattgcga ggtgaaacaa atatggcaat aaaatcttat
     5521 attaaacggt tattgcttta tatccgtcat ggagatccca aaataaattt agatgtgagt
     5581 tatttatctg aaggtgcaat gcttgctggt aagactgtga taattacggg tggtgcaaaa
     5641 ggacttggtt ttgcaattgc aaaatcattt atagctcaag gcgcaagcgt tattattaca
     5701 ggaagagaca tggattcgtt gaataatgct aaatgtcagc ttggagatat gtgttctatc
     5761 agggcgtttg atataactga tgctaaaatg tatcgaacgt tttttgaaga tgcatacgag
     5821 acctttggac gtattgatat actgcttaat aatgctggga tctcgttaca cgaagatgga
     5881 tttatgagtg ttactgagaa aacttgggat aagcaaatgg atacaaattt gaaaggtgtt
     5941 tatttcttta cacaggaatt tattaagttt gttaaagaaa agaatcaaat gcaagcaaat
     6001 attattatga tgtcctcaga aagaggcttg tgtggtgata gtattccgta tggattatca
     6061 aaggctgcaa ttaactcttt agttgtaggc ttgtctaaga aatatatttc tgagggaatt
     6121 agggttaatg gtattgctcc tggagtaact gcaaccgcta tgactggtat ttctgaaaag
     6181 ggtaatttgt ataatcctgc aacgatcggt tatagatatt ttctcccaga agaagttgct
     6241 caagttgctt tgtttttggc aagtagtctt tcaaactgta tcagtggtga aatcatagcc
     6301 tgtgaccagg ggcaatattt aagtcgaaat tgggggtaat aacttgggaa tattattaaa
     6361 aaaatataat agccttagtg agcctgcaaa agcatctatt tggtttacac tttgtaatgt
     6421 gttgcaaaaa ggaattctat ttttgactac acctattttt acaagaatgc tttcaactgc
     6481 agaatatggt aagtatacga tttttatatc gtggtattct attatagcaa ttttcacaac
     6541 gttgaattta tctgcaggtg tctataataa aggactaatt aaatacgaga ataaatatgc
     6601 attaacctcg tcatttttag gattatcgtc atttatttcc ctttgtgcat tgatgctata
     6661 ccttccattt agaacggtta taaatgggct actggattta tcgacggagt ttgtcatatt
     6721 gattcttatt gaatcaatat ttgagccggc ttttttgctt tggtcagtta aggaacgatt
     6781 tgattacaag tatcaaaaac tagtactcat cacagttctt atggctgttt taaatccagg
     6841 tctcggtatt atattaatct tatcatttgg ggagtatgct atttggagag tacgagctta
     6901 tgtcgtggta aatgttgtag tggggatgtt tattggcata ggaatactgg ctaagggtaa
     6961 gaaattattt gataaagagt actggacata tgcgctcaga tttaatttgc cattaatacc
     7021 acattattta tcaatgacat tgctgaatca ggttgatcgc cttatgataa atagtatggt
     7081 tggagctagc gaggctgcga tttatagtat cgcttacact atatctagta tgatgaatat
     7141 cataacaaca gctataaacg gttcttttat tccttttaca tacaaagcaa ttaaagatgg
     7201 cgtgtatgaa aaaattaaat ccgttgctaa tcaactatta gtgttgattt ttgtcttgtg
     7261 ttcgtttact atgctgttcg ggccagaatt gatttgggtt gtcggaggag aaaaatatat
     7321 ggatgctgtt tggattattc ctccagttgc ggcatcagta tttttcatgt ttttatatcc
     7381 gctttttggc aatattgaat tttactatga aaagacaaac tacgtaatgt ttgcatcttg
     7441 tgtgggctct atcgcaaaaa ttatttcaaa ctatgttagt attccccgtt ttggatatat
     7501 ttcggctggt tacacgacat tgatttgcta tatgttgttt tcctttgccc actacatgtt
     7561 ttataaaaaa attcttaga
//
LOCUS       DAYYHB010000109         7305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000109 DAYYHB010000000
VERSION     DAYYHB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.111"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..283)
                     /locus_tag="ACHA6B_002399"
     CDS             complement(8..283)
                     /locus_tag="ACHA6B_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949943.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            515..1249
                     /locus_tag="ACHA6B_002400"
     CDS             515..1249
                     /locus_tag="ACHA6B_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8949944.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1288..2430
                     /locus_tag="ACHA6B_002401"
     CDS             1288..2430
                     /locus_tag="ACHA6B_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949945.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(3058..3543)
                     /locus_tag="ACHA6B_002402"
     CDS             complement(3058..3543)
                     /locus_tag="ACHA6B_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8949946.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(3545..4216)
                     /locus_tag="ACHA6B_002403"
     CDS             complement(3545..4216)
                     /locus_tag="ACHA6B_002403"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949947.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(4479..5672)
                     /locus_tag="ACHA6B_002404"
     CDS             complement(4479..5672)
                     /locus_tag="ACHA6B_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949948.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(5756..5956)
                     /locus_tag="ACHA6B_002405"
     CDS             complement(5756..5956)
                     /locus_tag="ACHA6B_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8949949.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(6380..6613)
                     /locus_tag="ACHA6B_002406"
     CDS             complement(6380..6613)
                     /locus_tag="ACHA6B_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949950.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(6620..7051)
                     /locus_tag="ACHA6B_002407"
     CDS             complement(6620..7051)
                     /locus_tag="ACHA6B_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8949951.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         2200 a         1542 c         1333 g         2230 t
ORIGIN      
        1 tgtttactca acctttatac cgttccatga aactatcaat atcagatttt ttaaatctaa
       61 tgaccgtatc aatttttata acaggtaacc cgtattgaga aacatatttt ctaatcgttc
      121 ctactgatac acctagatat tcacttgcct gctgatagtc catatactct agagtgactc
      181 cagaaataat gtcttctagc ataggcttta gcactgtaat tatctctttc acttgttgtt
      241 caacttgtat agaaacaatg ctatcaatga tttgtagaga catatttccc cctcctttcg
      301 tgattgcttg aacaaatttt ctaagaatta tcttcgtatt ttttgctcaa aataaaagta
      361 ttttgttatt aaccgattaa tcaaacaaaa aatgattacc agttcatcga catacacata
      421 atacctcgat aaaaaagggg tgtcaatagt tttgtaaaat ttatttacaa cacccctttt
      481 ttgaggtata ctaattttag gaggtgtggt caatatgata aaacgtccac caataaatta
      541 tttagaacga aaaaagatac tcggaacaaa aataaaagct ataagaaaga gtaaaaaatt
      601 aacacaacca gcatttggtc ttatgattaa caacggtcaa ttaattgaca aaaagactat
      661 ctacgaatgg gagaagggaa cctatttacc aataccagag cgattatccc gaattgctga
      721 tttgggaaat atgagtatag aggagttagt ttgtggcaat gtagaagaat atattttggg
      781 aattattctt tatagagata gcattgtact agatggtata acatttccag ataaaaacct
      841 gttccagcat cttcgtcaac agttcccacc agtacactct aatttagata catggctgga
      901 tagatattcc aaattagaac cagagatgca agaatttatt gctaacaaga cttgtaataa
      961 agttaaaaat gaaaaaatta gtcttttcaa tatcttaaaa atagaagaat tattcatcaa
     1021 tgctattgta gaagaatttg acaataacat tcttttcctc actagcagta tagaagaact
     1081 actagaaaga atggtagatg aatggctacc tattcaattg aaagacatgt cttatcccga
     1141 agaagctgtg agagaaatta ctgataatat aaacaagcta gaacaaacga tttcatcaat
     1201 tgggaaaaaa tacactaaga aaaaaatgaa gggaggtgat accatctagt ggtaagtact
     1261 ccttgtaaac taagaggaga tgatttcatg gcaaaaatat caaagtacaa aaagaaaaac
     1321 ggtcaagtgg cgtggatgta taaaggacac attgccaccg atccacgaac tggtgaaaaa
     1381 ataaatacta cgagaagagg ttttagtagc aaacaagaag ctcaaaaaga ttatgacgag
     1441 tacagacatc aaatattata cggtgttaaa aagaaagcac ctgatatgac atttgaagac
     1501 ctgtataatg aatggataac tcatcagcgt accagtgtta aagcttcaac aatcgcaatc
     1561 tcagtaagat acgcaaataa tcaaatactt ccagcatttg gtaagcttaa actatcgaat
     1621 atctctgtcc catattgtca aaaagtggta gatgaatggc acagtaaata tgaaagttac
     1681 gattatatga gaaaacagac tgcacaaatt ttacgctacg gggttgcaat gcaatatatt
     1741 gacaataatc ctatggaaaa aacacttctt ccacgaaaaa aagagtatga gaaaaatcgt
     1801 aaattttact caaaagagga gcttaataat ttactggatg cttttaaaga ttttggtaac
     1861 atgaaacaat acgccttctt cagattattg gcatacacag gtatgagaaa gagtgaagta
     1921 ctcgctttac agtggaaaga catagacacg tttaataaag aactacatgt caataaaact
     1981 cttgctgtag atgaatttgg taaagttata attcaatctc cgaaaacaag agcttctaga
     2041 cgagttatct ctttagatac tgagactctt tctatcctca acaactggaa attacagcaa
     2101 aaagaagagt atttaaaact tggatacaat actagctcta aagagcaaca tgtttttaca
     2161 acagttaaaa acactctata cattccaaac actgttaatg attggttaag atatattttg
     2221 aaaaaatata acttgcctcg tattacacct catgggttta gacatacaca tgctagttta
     2281 ttattagaag caggagaatc tgtcaaggta gttcaacaac gattagggca tgaaaatagt
     2341 aaagtgacat tagacatcta tgcacatatt acaaataatg cacctaagaa aacaggtcaa
     2401 gactttgctg atatgatggc tcatcagtaa aagagctggt caaaatctgg tcatttctag
     2461 tatacaggca aaacaaaaaa gctagaaccc ttatatatca agggttctag ctcctataac
     2521 aggtttaccc aacactgcct tccaatgact cttttgggaa tcagaggaaa gaatcaggct
     2581 ttatagagat atttcatcta acaaattact ttagactgac agtatatatc agcaggtact
     2641 gtcatcggga acgtagccaa tatcgtatcc aaaacagcag gtagagaagc tatttgagga
     2701 ttcaagttat tctatttcaa ccatgaagtt aagttcatta aaaataaaga tagttggtat
     2761 ttcaataatt tttgtataga gttaatcact ttattgatgc taattcatct tgattggtat
     2821 tataatcgta ttcaagttgc gtggcgtgct tttgctagaa aatatataac tgttctttat
     2881 aaagaaatat aaggattaaa cattttttat catagagttt tacttatcta tagaattatg
     2941 tctgttccta attcagtttg atgaattaac tgataaaaaa atatctacta tttaaagata
     3001 cagggataag taagtaaaaa aataacatgt cattagatag tagacagttc caactcttta
     3061 tttacctttt ttgtatgctg cactgtcaat ccaagttcct aaattgttgt atccatcatc
     3121 acttatataa tcatattggc catcaataat ttcgtcaaac cacaaatatt ctccatctgg
     3181 tttttgccca attattttaa ttactgattg actagtagtt gtcatctggt taaaatcttt
     3241 tattttacct atattaacgg ccaaaatagc atttcctcgc ttataactct cctggatttc
     3301 atatttaaca tattctcgat caagagtttc tttacctatc agcaccacag tcactgatgt
     3361 tccaattaat tgattatcta tccagttttt tatagcaata tctccattac gtttaacttt
     3421 ctcaaattca gccttatcta caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt
     3481 tctaactacc attgctcttg ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac
     3541 catattaatc ctcctgaata ctatttatta aactgacaat ttgttcaact tttttatcta
     3601 cttctccttc agtatctaat ttttctagcg cttgaaattt actttttaag gaatcatcaa
     3661 aataatcact gaaattctct tgaatcttat caaacaaaac cttacttgca cctttggtaa
     3721 atccaatcgg aactactata ttttcatttt gaacagataa ttcaaattct tcaatcattc
     3781 catctgctgt aactatttca cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc
     3841 cagactctgc aataatgttt tctcgatatc ttctccactc atgaggcata tcaaggggaa
     3901 gtggtcttaa agaaagtacg tcttgtatgt tattttttct taaatttctt ctctgcttta
     3961 gaactcctga aacaatacta ttaccaattc caagtccgta acctgatact attctataac
     4021 cggattcaga aagcctatat gctaatttct cgacaaattt taatcctttc gattcagatg
     4081 taaatggagt ataatcaaca gcacttcccg aaataaaaat agtttttctc aaatactttt
     4141 ttctaatact tttcaaaata tcagtaatct cagaataatc atctacttca acaacgttta
     4201 tcgaaaatct ggacaaatca ttgatttgta gtcttctttt tgtcagttca taattatatc
     4261 tctcttgatc aaaaaaatcc tcagaatcga caaaattttc ctttaaaatt atatgggatt
     4321 ttagattatc tttttagaag ttcttgaatg tcggtatagc ccgtgtttcc gggcttttcc
     4381 ggcatttttg atattgttag atacttacaa atgttcttat tacctctcac catttccccc
     4441 tcaaaagtag taaatggagt agtaaaaccg gccttgtgtc aggctgccag ccgttccatt
     4501 tctgcccttg cggaatcgaa tgtggcgtgt gcataatagt tcagcgtcat ggtgatattg
     4561 gaatgtccca taatgtactg caaggctttg gggttcattc ccgcattggc taaccgggtg
     4621 cagaatgtat gacgcatagc atggggcgtc gtcgttttcg gcaacgcttc ttcatggctc
     4681 ttgttgtact tcttcacaag cctgccaaac atcccgccat aatctaccgc cgtcatggga
     4741 tagccgttct gtttgagaaa caggaaattg gcatacccgt caataatgaa cggctttgca
     4801 cctttgcggt tttttatcac tcgctggaac gcctgataca cttcctcatt catggggatt
     4861 tttctgacgc cgctttgggt cttgggttcg tcaatatagt aaccgacgcc ggtatttctc
     4921 aaaagctggt ggctcacgat gattacccgg ttttcaaaat ccaaatcttt gtccgtcagt
     4981 ccgcacagtt ccgaaatgcg aagccctgtc cccagcagaa taacgattgc gtcataatgc
     5041 ttttggtaaa ccttatcacc ttgaataaag gacagaaaac tttcttcctg tgctggtgta
     5101 agaggcacct ttggttccgt atcgtcgtcc agaacgtcac taagctggaa gtcaaacggg
     5161 ttttttcgga tgcaatcgtc ctgtatggcg gtgtaaaaag ccgctttcag ggaacgcttg
     5221 tcgttattga tggtcttata agaaaggccc ttttctttca tccgaagcgc ccattccttt
     5281 gcgtcggaca gcttcacact gtcaatcggg cagctcccca gccgatcttg ttccagaata
     5341 cgcatcagcc gttcccggcc cagtttggtg ttgtgcttca cattgccccg gtgcctgatc
     5401 tgcttggcgt agagctggca gacggtcatt ttcttgccga tggtgtctat cccgtcgtca
     5461 aggtctttct ggatttcttt ctccttttcc cggagtgaaa tatcctcccg tttacctgcc
     5521 ggggtcttat ccgtaggcac taacttccat gagtaaacaa actgcggttt tccaaaggta
     5581 tcaacatatt tgtaagcata tcgtccgtct tttctctggc tctctccaga ccgcaaaacg
     5641 cgatttttgc tgtctcgtct tttttctgac atctcgtcat gctcctttcc atgacggaaa
     5701 gagccgcata tatgcttata aattattata acatatctgc ggcccttttt cactattaga
     5761 ttgtgtccaa agtgtcaatt attttctcaa actgcttgcg tttgatctgg atgcggttgc
     5821 cattcataat gacccagcca gaggccggat tttcctcggc cagcttacgc aatttgtttt
     5881 ctccgatacg gaaatattta gacgcttcct caatcgtcag cgtgtatttt tcccaaatag
     5941 gcacatcatg gttcataatg cagccccctt tcacaaattc aaaggtttgt gtgatttagg
     6001 ggcatagacg gacggctgct ggcacagctc cacgggactt tcacccccgc gtggttctca
     6061 cgcggcccta cccattgcct gcgacgcttt taacgctcgg actgtggcta taagggagta
     6121 tcatagtcct gcctgtacgt cgtcgcccgc aagccgccac gctcacattg cggcgtggtg
     6181 ttcctcgctc cgcttttccc gtagggaaaa gtatcatggc gcaccagccg tccggctcgg
     6241 cacagacaga atgtgtccgt atgccctatg atgcgccgcc gttatcttgc gggcatattt
     6301 atcaaggtgc gatcaggcca ccgggaaaac accgaaacgg ggaaagggta agccgtttca
     6361 tgctgccggg gccggttcgt cagttcgtga cgatagcgtg gatgagcttg atttccagac
     6421 ggcgcttcat ggcctcgtcc agacacacat gcggatagcc gttttcgtca tagagcgtcc
     6481 gggtacacag cttgttcatg taaccgctgt aatactgcaa aacgcgctct acggctccgg
     6541 catccccagc gcgggcggcc tcgatcaccg aaaggggcaa aagctcaatg cccttgtagt
     6601 tcgggttctt catccgcgtc taacctccct tcggcatcaa cgcctttagc tgtttccgca
     6661 gctccgataa ggtatttgtc ctgtgccgct gaacggcact ccgggacatt cccacaaggc
     6721 cgccgatctc accatctgcc atatccagaa cacagtgcag gattaaaata ctctgctcca
     6781 tgctgggcag cgcggcaaag gcttcggcaa caagctcatt gttgatatgt aggtcatagc
     6841 catgcgacga aaacgtaaag ctgtcgctgg ggtagtgatc tactgtacag agcttgtcca
     6901 aatctgcctg tgacaaagag cttagtgaca cttcacggtc acggcggcgc ttcatttcag
     6961 ccaagtagct tttggcctcg ttacgcagta ccgtcttgca aaaagcatcg aacatgcacc
     7021 gcgcttcata gtcgcggggg atagcatcca tcttctcacc ccctttctgt gggggactta
     7081 ggtggttctc tccctttccg ctaacaagac tttgacaagg ccgccagcta cccgctaaat
     7141 acctcctttt tgaaaatttc tcaaaaaatt tttttgggga cagccgggcc gcaaaagacg
     7201 acaaaaatcg cccggcaggt cgcaaaccta ccgggcgaga aaatgctttg ctatgctgct
     7261 gatttacatg gtatagtaca tactcataac cggaatagcc ctgtg
//
LOCUS       DAYYHB010000110         7173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000110 DAYYHB010000000
VERSION     DAYYHB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.112"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..545
                     /locus_tag="ACHA6B_002408"
     CDS             <1..545
                     /locus_tag="ACHA6B_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949952.1"
                     /translation="IPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGID
                     ETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLI
                     SPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEE
                     KILLPENWAELRKLVEYGII"
     gene            532..1953
                     /locus_tag="ACHA6B_002409"
     CDS             532..1953
                     /locus_tag="ACHA6B_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8949953.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1966..2973
                     /locus_tag="ACHA6B_002410"
     CDS             1966..2973
                     /locus_tag="ACHA6B_002410"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8949954.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2985..3989
                     /gene="neuB"
                     /locus_tag="ACHA6B_002411"
     CDS             2985..3989
                     /gene="neuB"
                     /locus_tag="ACHA6B_002411"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8949955.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3986..5134
                     /gene="neuC"
                     /locus_tag="ACHA6B_002412"
     CDS             3986..5134
                     /gene="neuC"
                     /locus_tag="ACHA6B_002412"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8949956.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5107..5784
                     /locus_tag="ACHA6B_002413"
     CDS             5107..5784
                     /locus_tag="ACHA6B_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8949957.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5735..6916
                     /locus_tag="ACHA6B_002414"
     CDS             5735..6916
                     /locus_tag="ACHA6B_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8949958.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            6929..>7173
                     /locus_tag="ACHA6B_002415"
     CDS             6929..>7173
                     /locus_tag="ACHA6B_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8949959.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEA"
BASE COUNT         2585 a          924 c         1326 g         2338 t
ORIGIN      
        1 aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa catacaatca atctttggga
       61 tagaagaagt ttttgtcgga gataaatgta tcttttttga tcaaccttta ttatctgatg
      121 gttatggcat agatgaaaca gatattttcg ctaaagtaaa tagtatattt tctggaaaaa
      181 aagaaacttt aattaagctt catcctagag caaggacaaa tcgttatggt gaaaaaaaca
      241 tattgaattc cagtttaccg tttgaagtct tgctacttga aaatgacttc tcttcaacac
      301 ttttgattag tcctatatct acaatttctt tttcaccatc tatgatgttt gaaaagacga
      361 tcaactctat tatgttggct aaattaattt taaatgaata tacagcatta tctgaagata
      421 aaaaagaaac attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag
      481 aaaaaatttt actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta
      541 tttaacacac tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat
      601 ttttttatta agggaatttc attcataact attcctattt ttgtaaatat tactactcaa
      661 gcagaatatg gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt
      721 gggctaagcc taaattcggc aattaataat gcaagttttg actataaaga tgacataaat
      781 gaatttatgt catcggtact ttctctagct acactttcgt taattgtgtt cttattaata
      841 gggaacatat attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat
      901 ttgatgttat tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg
      961 attaacttta accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt
     1021 gcaatctctg tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta
     1081 gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata
     1141 aagggtgctc atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg
     1201 ctaatcccac attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat
     1261 tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt
     1321 ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg
     1381 agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact
     1441 ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa
     1501 gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt
     1561 ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt
     1621 ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta
     1681 ggaataattg ctgctggata cacaaccgta tttacgtatt ttttactttt tatttttcat
     1741 tataatatcg ctaaaaaaat tacgaataaa caattatttg acacaaaaaa aatatgtatc
     1801 ataataattt ttgtatcaat tttttctttg atacttcata ttttaatcaa tcaaatgttg
     1861 attcgttacg ttttgacatc attattgata attagtttag ttatatattt tttaaagatt
     1921 aagaatcgaa ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa
     1981 acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta
     2041 aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg
     2101 caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct
     2161 caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa
     2221 cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact
     2281 caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca
     2341 gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta
     2401 ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt
     2461 ggaaatgtca tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac
     2521 aataaagata ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat
     2581 gctgcaagat tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta
     2641 aaaatgccgg caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat
     2701 acacatgatg aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa
     2761 ttatatgaag agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg
     2821 tatatcattc caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt
     2881 aaaatcactg catactcttc agatgcaaca gaattaatga gtggacagga atttattaga
     2941 atcgcaaaag agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat
     3001 atgtaattgc agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta
     3061 ttgataaagc taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata
     3121 agctagtttc caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca
     3181 gttctcaatt tgaaatgcta aaaaaattag aattatctca atctgatttt attagaataa
     3241 aagattattg tatacaaaat aaaattgatt ttttgtctac tccttttgat gaagaaagtg
     3301 cagactttct aaaacaaatt ggtgtacaag gatttaaaat tggctcagga gatttaacaa
     3361 atttaccttt tcttagaaaa atagatatgt atgggttgcc agttttatta tctacaggta
     3421 tggctgatat tcatgaggtc agtgaggctg tagattgttt catagatagt ccagttacta
     3481 ttttacattg tacttcaaat tatccggcta gttctgaaga tattaattta ttggcgatta
     3541 acacattaaa agaaatgttc gggatacccg ttgggtactc cgatcattca ttgggatatg
     3601 atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg
     3661 ataaggaact acctggtcca gatcataaag cttctttaga tcctcaggaa tttgcagatt
     3721 ttgtaaagca tattagaaac actgaaatat ttttaggtga tggtgttaag cgagcaatgc
     3781 cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct agtggtcact gagaatttaa
     3841 atgtgggaga tattcttaca gagaaatctc ttacaataaa acgtcctgga aatggaatag
     3901 ctcctaagtt tttatatcaa tatattggca aaaaagttaa aagaccaata agtgcagata
     3961 gtgttctgct tgaggaagat gttgaatgaa aaaaatactt atagtaagtg gaacacgagc
     4021 tgattatgga atatataaac ctgttgctga gaatttacta caagataatt gggatgtatc
     4081 atttgttgtt tctggaatgc atttatccca taaatatgga tttactaaag aattgatctt
     4141 agctgacgga tttagaatta gtggagaagt tcaaagtttg tttttagaga acaataaggg
     4201 aaacatggct cggtctgtgg cattagagat aaatggtttc acaaatatat ttgaacaaac
     4261 tagccctgat tttgtattat tactagggga tagaggagaa atgttggcag ctgcgatagc
     4321 ttgtttgtat cttggaatca aaacagcaca tattcacgga ggcgaagtgt ctggtactgt
     4381 agatgaatca ataagacatg ccataacgaa aatagcatct gttcattttg ctgctacgga
     4441 aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct tggagagttt ttcccgtcgg
     4501 agctcctaga atagatacta ttttaaataa tagattacct agttttagag atataaaaga
     4561 aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt
     4621 aaccgaacta gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca
     4681 atctcaacca attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa
     4741 aatgtaccag aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga
     4801 atacctgact atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat
     4861 tgaagctgca tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga
     4921 acaatctaaa aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa
     4981 taaaatacaa gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga
     5041 cggcaacagt tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga
     5101 tattaagtgg attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt
     5161 attggggctg gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta
     5221 atatatggat tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa
     5281 ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct
     5341 attggtgact gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa
     5401 tatggaatag ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga
     5461 actgttattt ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata
     5521 ataaacacag gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct
     5581 ccaggtgtat gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt
     5641 tctcaatgta tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact
     5701 attgttaagg atattccctc acattcctta gcttatggca atccagctaa aattaagaaa
     5761 gaaggaatta attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga
     5821 agaaaaatat gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt
     5881 ggataaattt gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt
     5941 aagttcaggt acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga
     6001 tattgtcttt gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg
     6061 ggctgtacca gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact
     6121 agagagcgca ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt
     6181 gaatttatat ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa
     6241 tgttccagtg atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc
     6301 aggcttattt gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc
     6361 aggtggagga gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc
     6421 aacacaagca agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg
     6481 tctaagcaat atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt
     6541 agctaaacga agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat
     6601 tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat
     6661 caatgattat aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa
     6721 cattgaaaca agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa
     6781 aatgtattac catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg
     6841 cttaccttct ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa
     6901 aaaaatattc aagtaggtgt gatattttat gaacatagga attattggtt taggttccat
     6961 ggggaaaaga agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt
     7021 tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa
     7081 tagtctaaca gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc
     7141 tataacccat gaatttatta taaaagaggc ttt
//
LOCUS       DAYYHB010000111         7151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000111 DAYYHB010000000
VERSION     DAYYHB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.113"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..3187)
                     /locus_tag="ACHA6B_002416"
     CDS             complement(38..3187)
                     /locus_tag="ACHA6B_002416"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8949960.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3201..4418)
                     /locus_tag="ACHA6B_002417"
     CDS             complement(3201..4418)
                     /locus_tag="ACHA6B_002417"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8949961.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4408..6003)
                     /locus_tag="ACHA6B_002418"
     CDS             complement(4408..6003)
                     /locus_tag="ACHA6B_002418"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8949962.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6128..6913
                     /locus_tag="ACHA6B_002419"
     CDS             6128..6913
                     /locus_tag="ACHA6B_002419"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM8949963.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            6906..7148
                     /locus_tag="ACHA6B_002420"
     CDS             6906..7148
                     /locus_tag="ACHA6B_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949964.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2080 a         1452 c         1183 g         2436 t
ORIGIN      
        1 tatctttgaa tcgtacaatt ttcttaactc ttctcgttta cctcaattca ttttcaagtg
       61 gaatcaactc atcatctaac attttacgcc atgctcgctt catttcttgt gggtatttaa
      121 atggtttcgc ttctggatgt ttggctttat atccttcgta atccttactg ttgataattg
      181 ctttcatatt aggaattgga tcttcacctg gcatatattg cagattagaa gcatgtaatt
      241 ctttctcaga aacaaaccac tcattggaaa aggcatgaat caaattatca cttgcttgtg
      301 taaaatagcg acgtttaatg acaaagatat cttcattact atctaaagtc cccacatcaa
      361 atgcatttaa aatttgtcgg taaacttctt tgactccttc tgttttattc aatttagcca
      421 aagctttttc aatttcttct cgtgcatctg aactattcgc agcataagtt cctagtaatt
      481 gatcaatata agtagaatca atatcataca acttagtggt attttcacta taaaattcga
      541 tcgttgaaaa atcttctccc tctttttctg gttcttcctc catcaatgaa cccttcacgg
      601 tctcgtacac gccaatttgt tcttccaaaa gttgaatttg attgttcaaa gttgaagatt
      661 gcaccatgtc atcattgtaa tcatcataag tcactaacga ttcatacgca cgattcaatt
      721 cttgaaatgc tttgacgtac tcgatacgga tttcaagtgg cgtatgttca tcaatggatt
      781 ccatggatga aacctctttt aacttaccat atgcttgctt gaatcttttt ttggattctt
      841 ggtaacttgg ataaatcaaa cccgattctt ctttagcttc tgaatacagt tttgtcgcat
      901 cctcgacatt cttctccatt gtcgctggct ttcggaaagt aacaattagt ccttttgttt
      961 tttcaggata cgtacggttc gtacgtgaaa aggcttggat caatcctgca tactctagat
     1021 tacgatccac aaatagggtt tgaatcgtag gggcgtcaaa acctgtcaat agacgatcca
     1081 ctacgattac taaatcgatt tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca
     1141 aacgattatt gatgtctccg ttataacgct caatgtctgc tagtgaccag gcagtatcat
     1201 aatactgatt atattcctca ataatttttt gcatttcttc ttgttgggca cttgaatctc
     1261 ggctattttc ttctaatgaa taggtaatag cgattcgtgg gaaatcagga tcctccatcg
     1321 ttcgtccttc acggataggc tgattgggaa actcttttat taaccaatca gattctttcg
     1381 tcatctcttt gatggcccga taatactttt tggccatatc aatggaactc gtggttaaga
     1441 tcgcagactt cgttggacgg ccatttcgaa aatcaaattt tgtataggca ttgtctggac
     1501 ggaacatttt atgaatgact ttttggatat gttcctctgc ttcaaaaaca gtagggtcca
     1561 aataactttc ttttttgaca gactccattt gatcgatcat acgattgatc tgttccggtg
     1621 aatactctgc atatgtttct acttctttta atttccgata gattttattc tctaatgaag
     1681 tcggctccac ggtgttttca tgctctactt gaaatcctaa aaccgagcca tcttctaatg
     1741 cattcttgat ggtataggtg tgcaagacct ctccatactg atcatgggtc gtacgtgcca
     1801 attgcccttt ggcttgtttc cgattctcag ggaaaatcgg tgtacctgta aagccaaacc
     1861 aagtggattt cggaaacatt tttttgattt cttccatatt ttcagcactt aacgctcgat
     1921 gacactcatc aactacaaac acgatatgtt gccccattaa tttttggaag cgattggttc
     1981 cttttttctc ttcctgtttc tttgcatatt tcaacgccgc atctagtttt tgacgggttg
     2041 taatgatcac aacattcgca ttggcatctg ccaacagagt ttcacttaac tctttggcac
     2101 ttcctgttcc cacgatcaag ctattcgctt tggcattacc agaagaaacc ccagtattaa
     2161 actccgaagc aaatttcgta aactctgtgg tggtctgatt atctaaatct ttgcggtcca
     2221 ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa aagcttagta gatacaaaac
     2281 tagtcaacgt ctttcctgaa ccagtcgcat gccacacata ccctgattga tgtttatttg
     2341 cggcaataaa caaggcttga atcgcatgca cttgataagg atgtaacacc atgagggttt
     2401 tattgtcttg atcttcactg acaatcgtat aatccgcaat caagcgatgg gcatctggga
     2461 tatttagcac ttgtttgcaa aactcatata agttttctac ctttttatta tctttcgttc
     2521 gccaactaaa taaaaactta ggatgcaaat ccttcggtaa ggcatttgca aaataccgag
     2581 tcgtttgttc attggaaacc acaaacaact gtaaggtcga aaagatattg ttccgaaaca
     2641 tgccttcttc agcatatttt ttgatttgat taaacgcctg ataaaaacca tcttttgcag
     2701 ttacttgttt tagctcgatt tggataatcg gtaagccatt gatcaataag gtgacgtcaa
     2761 aacgtcgatc ccgcgcttct atgttacttc cacgttttgc aatttgatgg accacttcat
     2821 aagtagagat tcctccacca atgtcttgat tggaataaag gataagtgag acagaaccca
     2881 attgcggatt ttcccgttca atcgtgatcc gagccatccc attttcacct tttaaccatt
     2941 tcgcagcatc aaatggggtt ttcgtccgta gcaataattc ggttttgatc gtttcaaact
     3001 ctttatcggt caaaggagaa tcattcagtt ctgcttgatt atttgagatg attttttgac
     3061 gtaaattgct ccaaagatct tcttctgatt ttagatccgg acgatacgtc cactgattat
     3121 gtccttcccc gaggacacga atcaactgtt cttcgacttc cgcttcctca cggtgactaa
     3181 ttttcattct tcttccctcc ctaaacaaac atcttttgca agaagccttt tttcgtttcc
     3241 ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg cttgaagaag
     3301 gcaccaattt tttgttgttc atttatatcc ggtggaaact gaaatgtcct ttgttctata
     3361 ccacctttag tcacatgtac cattgtcgtt ccgttagttg attctttaat tttctcttta
     3421 tcagtcaata gccaagtata tagatactgt ttgtccaatt ccttagataa aaatttaagt
     3481 ttccaaatat gataatggta gattacacgt tcttcattcc acatttcagg accgaagctt
     3541 gttgcccata aatataagag atctccttta ttagcatact tgtcttcctc taattctaag
     3601 tttgaataat accatctatc attagtatta aaattaccta ctcttaaaac cctatatttt
     3661 ccagaatcaa gtaattcttg ttgtttaaaa gctctaccat tcaaaaaaac taccacttcc
     3721 cccaacttcc gctcttccca atctcccgta aatcctggaa aacgaacttc tggcactttt
     3781 gctccgtttt tagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct
     3841 aattcgtgct ggtgaagcat gatggtgtga tcaagttgtt tgaaaaatga accgatcttg
     3901 atctgttcgt cataagttgg aagtgagata ggtgtctttt taccactatc aattgtatag
     3961 gttccgacgg ttccaatgcc tgcaacgtca cggatatact tctgaatttt gttatttatg
     4021 aacgagtagt aaaagaagtt tccgtcaatt ttatgctcat tcttgaacca aatgatgttt
     4081 ccatccttaa agtaaattgg gttatcatca gtgattaata aggggacacc tattgatccg
     4141 acaccagtta caagcaagtc tccttgagaa actttacctg aatttttcga atattcatta
     4201 tatttttctt ctgaaatgta aagataatcg gatggttcct cattttttgc agctgagaca
     4261 atatcacgag cacgtaaaaa tctaatacct gagttagtcc aatctgattg atgaattcgc
     4321 ttcacagaag taacatccat catatctcct aacttacgct cttcccaatc ttccgtaaaa
     4381 ccaggaaacc ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     4441 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     4501 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     4561 atcaattggt tcttcttctt cgaaagtatc gacatagcgt gggatattta agttatagtc
     4621 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     4681 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat aatttattct gatttttccc
     4741 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     4801 caatacaatc accgtcgttg gaatcgatgt accaaagaac aagttcgcag gcataccgat
     4861 cacagcataa atactgccgt cttctaataa tttttgacga atgacccctt ctgcagcacc
     4921 acggaataaa accccgtgtg gcaagacgat agccatagtc cctgtttcct tcaaatgata
     4981 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccgtagcg
     5041 attaaaccga gaatcatcta agaaggtcgt atctgctgac caattagccg aataaggtgg
     5101 attcatcact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5161 atccccattg cggagattca tttcttcggc atctacccca tgtaaaatca agttcatttt
     5221 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctggatg
     5281 agttaagtag ttacgtacat ttaacatcaa ggaacctgaa cccatggttg ggtcaaagac
     5341 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
     5401 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     5461 cagaaattca tacgcatccc cgatcacatc cccatcgtga cctaaaactt ccacgtcatt
     5521 caatttcttg ataacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     5581 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     5641 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     5701 taagacgttg aaaagatact ctggtgagat cgaatacccc aaaatatcaa cgattgtggc
     5761 aatcaggtct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     5821 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     5881 acggtcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     5941 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6001 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6061 ctccctttta gaaaaagcaa aaaaaaaaga aaaaatctta ttcttgataa caagctacgt
     6121 gacgtatatg cctaacaaaa atagaacttt tgaatcaatc ttaaatgaag gtttaaccac
     6181 agaaccggca tccattattt ttgatgaagt tatcgatgac gttgaagata gtaagtatca
     6241 taaaaattta aaagaacttt tttattttgc actcaacaat ggaccaagtt tgttcactgg
     6301 tatatcagta cctgatagtg ataatccatt aattatggca aaaatttact taacaaaatg
     6361 gtgtaataaa tacatcaggg ataggaataa cccagcatta aaaaaacctc ttaaaacatt
     6421 tggcgaaaaa gatgctgctt taactactag agtagcagca aatgctaata ttgatgatca
     6481 aaaagtattg aatgactact tgcgtggaca ttttttatat atgagcgctg agaacatgaa
     6541 cggaagtatt ttagaggaat atttagccga agtgttagaa cctgaaggtt ggatttggtg
     6601 cgccggctct gtatatcgag ctgttgattt ctgttattta ggaacctcac caattctttt
     6661 acaagtgaaa aataaataca atacagaaag cagttcgagt tcagctataa gagtaggaac
     6721 aacaattaga aaatggaatc gtttaaataa gtcgacaaaa atttctggtt tggatagccc
     6781 tatccccaat tggaaagcat taatagaaat gactgaagct agtaaggaac tggcagctaa
     6841 gttaactgaa aatagttatt tagcttatat taatgaaaaa tccacacgag aattatggac
     6901 actcgatgac taacaatatc cttattagta tctatccacg atacatggaa aaaattataa
     6961 acaaacaaaa aaactttgaa tttagaaatt tctatcttac ctcagatcct tataatatct
     7021 tttggattta cgaaacaatc cccaccaaag ctttaaagta taaattgatt gtaaaaaatc
     7081 caattactaa acttaagaaa aatcaacact actttttagg agacgatgag tttgaacaat
     7141 tagtttaaaa g
//
LOCUS       DAYYHB010000112         6643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000112 DAYYHB010000000
VERSION     DAYYHB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.114"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHA6B_002421"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHA6B_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA6B_002422"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA6B_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8949965.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHA6B_002423"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHA6B_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHA6B_002424"
     CDS             complement(2143..2373)
                     /locus_tag="ACHA6B_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8949966.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHA6B_002425"
     CDS             complement(2603..3421)
                     /locus_tag="ACHA6B_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8949967.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHA6B_002426"
     CDS             3582..4271
                     /locus_tag="ACHA6B_002426"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8949968.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHA6B_002427"
     CDS             4285..5787
                     /locus_tag="ACHA6B_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8949969.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHA6B_002428"
     CDS             5800..6276
                     /locus_tag="ACHA6B_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8949970.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHA6B_002429"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHA6B_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAYYHB010000113         6609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000113 DAYYHB010000000
VERSION     DAYYHB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.115"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHA6B_002430"
     CDS             complement(1273..1938)
                     /locus_tag="ACHA6B_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949971.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ACHA6B_002431"
     CDS             2118..4100
                     /locus_tag="ACHA6B_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8949972.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHA6B_002432"
     CDS             4104..4742
                     /locus_tag="ACHA6B_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8949973.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHA6B_002433"
     CDS             4770..5198
                     /locus_tag="ACHA6B_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949974.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHA6B_002434"
     CDS             5655..6566
                     /locus_tag="ACHA6B_002434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8949975.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2275 a         1001 c         1219 g         2114 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaact
//
LOCUS       DAYYHB010000114         6536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000114 DAYYHB010000000
VERSION     DAYYHB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.116"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..519)
                     /locus_tag="ACHA6B_002435"
                     /pseudo
     CDS             complement(120..519)
                     /locus_tag="ACHA6B_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(516..752)
                     /locus_tag="ACHA6B_002436"
     CDS             complement(516..752)
                     /locus_tag="ACHA6B_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFM8949976.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEESDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(912..1196)
                     /locus_tag="ACHA6B_002437"
     CDS             complement(912..1196)
                     /locus_tag="ACHA6B_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949977.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(1367..3595)
                     /gene="topB"
                     /locus_tag="ACHA6B_002438"
     CDS             complement(1367..3595)
                     /gene="topB"
                     /locus_tag="ACHA6B_002438"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8949978.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(3769..4179)
                     /locus_tag="ACHA6B_002439"
     CDS             complement(3769..4179)
                     /locus_tag="ACHA6B_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8949979.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(4296..5459)
                     /locus_tag="ACHA6B_002440"
     CDS             complement(4296..5459)
                     /locus_tag="ACHA6B_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8949980.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            complement(5637..6491)
                     /gene="aroE"
                     /locus_tag="ACHA6B_002441"
     CDS             complement(5637..6491)
                     /gene="aroE"
                     /locus_tag="ACHA6B_002441"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8949981.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT         1753 a         1375 c         1071 g         2337 t
ORIGIN      
        1 attaatccat attttccata tttttatgaa aaaactattt tgtatgatta aacataaaaa
       61 aatcagcata ttccttaatg acatgctgat ctaattgaga aataaagttt ttaattcgtt
      121 cattttattg cggtttgtcg taaataactg gttatcttgg actttagtga atcgaaatcc
      181 aatgaactag tggtaatcat tatgataaaa tcaacagctt cttgacgaga aaaagcaact
      241 ggacaattat tcaattgaag aaacaacaac atggcaacaa gagcagttcg tttattgcca
      301 ttatgaaaag gatgtttttt agctaaatta attgctaaaa tcgccgcttt ttcatagatt
      361 tcaggataga gttcttgatt gaatactgct tgtgaaggct gtttgacggc catatctaat
      421 gcatttgcat cttttacacc gatcatttcg cctggcgagt gacggtaaaa taactttcgc
      481 gttgattttg ataatttctt tagtcgaaag atacctcata aatcaaccaa atcttttaac
      541 gcgtcttcat attgattcat gatcgaatca actaatgaat caatatcaga ttcttcttgt
      601 gctttgatca ttcgtacaga accatcagat tgaaaaacat aactgattgc gtcacctgtc
      661 tgcacaccca tgaattcttt tacttctggt gggatagttg ttacatcgct attgccagat
      721 tttctaattt tatacttttt catttttttc atatttcgcc accctctata ccattctcat
      781 tctatagtaa gtacattata tccaagtata tacttttaat caatgtatat acttttattt
      841 tgctttttct tacgaaaaaa tgacaaagaa aaaagtttgt gacatgactt ttgtcacaaa
      901 ctctcgtatt cttaaccaaa atattgatga aatacttgtg taataactgg tagaaaataa
      961 ataatgatca tagccgctac caccaccaac aattgttttc gctgtttctt tcgctttagc
     1021 atgcttgtcg ttttatgaaa aaaataagta gcgcttttag ctcctgcagt caaagtcgat
     1081 aaaatcaacg ttggtgaata tgctgtttga ttgtgcgtca atgacaacaa cttcgtcccg
     1141 taatcaggat actttagaaa tgcttggtac acagccacaa tcgtgattgc tcccataaga
     1201 taatttaaaa atgaatgcac ttttttcaaa taaaaaaact ccttgctatc tatataaaat
     1261 cagcctaccc caacgttttt gcttgattca ctgatatttt ttagttttat tggtatttat
     1321 attctcactt ttcggaataa atattccgct atttcattgt tttcatttac tctacttcgt
     1381 tttcattatt ttttcttgat gtttctttgt ttccatcaac tgcttttgtt ccactgtttt
     1441 cgatccatgg aaaagaaaaa ccgattgtca tatcttcttt taatactaaa actgcatcga
     1501 atttcccttt tttaccagta aaacctttga caactttact tgtctttcct tttgaaagca
     1561 aatctctgag attcgtttta gtcagcttct ttttagctac tgttggccac aatttgaagt
     1621 cgcattccgg attcaagcat gtagcgattt taggataaag catgactatt ccctttttac
     1681 attttggaca tcttcctaat tgatcgtttt tcttctccat cgcttttatc tgacgtgtcc
     1741 cgctaatctc atctgaaaag tctaccttgt cgatttgatt aggaacttct gccaataaat
     1801 gtaggatgaa ttttttgata ttctctaaaa aaacagatgg tgtcccctct tgtttaccaa
     1861 tttttctcaa gtacccttcc cagcgcgcag tcatttctgc gctcgtcagt aatggttcat
     1921 tggctactgc cttacaaaga gtgattcctt ttggcgtaac agttatttcg tttttattga
     1981 cttcgatgta atgcttctgc ttaagatttt caatgatatt ggctcgtgta gcttctgtcc
     2041 ctattccttc tacttctttg agaatttctt gcgcttcatc attatccagc gtttttcccg
     2101 cagttttcat tgctgtgatc aacgttcctt cggtataggg ttttggaggt tgtgtttctt
     2161 tctccatact tttcagttct acttctgctt cttcattttc tacgatagga ggaagagtct
     2221 gagcttcttt cttttttgac ttgatgcgta agatttcctg ccaaccttgc tgtaatggga
     2281 tctttccgat gctttttagc tttaattgct gtacgcccgt cattacggtc gtctcttcgt
     2341 agatataatc tgcagcaaac atagccacag tagtgcgcaa aacttgcaaa tagattgctt
     2401 gctgtaattt ggaaagcttc gcaaagcgtg caggagtcgg aatcgtcttt gtcggaataa
     2461 ttgcatgatg ttcttgaact tttttcgcat cgacatatcg tttctttgct ttgatctcag
     2521 ttgtctgtac ttcttctatt cctaaaaatt ttttataatt ttgcagatgt ttaaccagat
     2581 actgatgctc attttctgta atgtaaaagg aatccgttct tggatagctt agaaacttgc
     2641 tttcgtacaa tccttgagca gcatcaagtg tagctttggc acttgctttc attagctgat
     2701 tcatctttgt ctgcaaacta gatagcgaaa ataaacgtgg actatgcaac ctcttttcct
     2761 ttttcgtaac ttcaagaatc cgtccgtttt gcttacctaa atgcgctcca agtttttcta
     2821 tttccttcgt taactcttct tgggttttaa atgctttttt tggtacgatt tttccagaaa
     2881 actgcccttt gtttgtcgta attaaacctt ctccttcaaa gtacgtttct tttttgaagt
     2941 gtttgatttc ttcttgcagc tgataaatca tatagagtgt cggtgtttgt acacgaccaa
     3001 gagaaaaact gccatcaatc ccttttcgtt gaagtagcaa gctatataat ggacttccat
     3061 tcattccaat cagccaatca gcgttctgcc ttgcttgcgc ttctttatat tttggaaaat
     3121 agttctttcc aggttggaga ttcttaaaac cttcatagat tgcttctttt tcaagagaat
     3181 tgatccataa acgtaaatag ttcttttcct tagaaaatgc gtttgcttgt cgaataatcg
     3241 accacgcgat ggcttcacct tctcgatcac catctgttgc aataatgatg gtatcggctg
     3301 actgcaattc tcgttttacg atcgaaaatt gtgcacgttt gtccattgga acttcgtaac
     3361 ggaaggtttc tgggaaaata ggcaaatttt ctaaagacca ttttgcccaa cgctcttcat
     3421 aagctcctgg agcaaccata tctaccaaat gtccaaaacc ataagtgatg gttacttcac
     3481 cagcaaacaa tggatcgctg atttcataat acccttcatg ccgcttttgc tgttggaagg
     3541 tttcggcata ggcttttgct tgtgaaggtt tttcagctag tatcaccgtc ttcatcttct
     3601 gacctccgtt acttaatttt ttgcggactt ctctattatg gaacatattt tcgcatctgg
     3661 caataaaaaa aagataccgt atagtcgaaa tagacacaaa aacatcaagc aaacaaacga
     3721 tgatccgttt gctgcttgat gtttccagaa aatattcatc tttagctgtc actcttggtt
     3781 aatctgccag ttgatgccaa atcgatcagt taccatccca tacaactgag cccacggaac
     3841 agcatccagt ggcatctcaa tcttaccacc ctcagctaag ctttcaaata ggcgcttcgt
     3901 ttcttcttca tttcttgtct ggatgactaa agaaacatta tttcccacaa ctaattcagg
     3961 accaattcct tctggcgtat ctgcaaacat aactaatgta tcataaatgt tcagccgtgc
     4021 attcgtgatt agattttttg cttcttctgg caagttcatt tgttctattc caggcatttc
     4081 accgtatgtg gtgatacctg atgcttcagt ctgaaaaact ttttcataaa aagcgatggc
     4141 ttcacgcgac tcatttttga agttcagata gatatccaat gtaaacagct cctctcttgg
     4201 ttaaatctac cataagaaag cgttttttac gagtgaatcg tttgattgat tcatgacaaa
     4261 agccttgcgc agcttcttct tgatcttttt tatgatcatt tattcaaaaa atctgtaata
     4321 gcagattgat tcatttgatt atcaatttca agaatactgc cagcataatc actttgccca
     4381 taattccacg aaccgtctac tggcacactt attcgatcaa tgccaccagc tcctcgtgca
     4441 atcgaaaaga ggtttttcgt caagaaccaa gcagtgacat ctgtggatgt atatcctaaa
     4501 gctttaccag ctgcataagg tgcacggatc agattcaatg gattcttcaa ttggctgaaa
     4561 atagcgttca ttacttgctg ttggcgtctg acacggccaa agtctccttg ttcatccatt
     4621 cggaaacgtg cgtactgcaa aagaacatgt ccatccatct tctggccccc ttttttaata
     4681 tctacaccat ccagattcag atctttttct gcatcgatct tcactccgtt catgaataag
     4741 gcatcgatca ctttttcaaa cgatttgaaa tcgactttcg catagtattg acaatcgatt
     4801 ccaaaatttt ctactaaagt ttgacgcact agatcggctc ctccgtatgc ataagctgaa
     4861 ttgatcttat tttgtcctac ccctgggatc gtaacgaagc tgtcacgcat aaaagaaatc
     4921 agtttcggct tatgggaagg accatccaac tgtaagacca tgatcgtatc ggctcgcccg
     4981 gcatcttctc cgcgtgtatc acttccaagg atcaaaatat tatgcgcacc attcgtcgct
     5041 ttttgcccat gaaaggtttc tgaagtcgct gcctctaagc ttgtgtcatg ttcagctaca
     5101 tatttaccaa taaaaaatga gatacctgaa taaaggatca aaacaagcaa aatcgataga
     5161 atgatttttt tccacgatcg ctttcttcga ggttttaacg gtttgttctt cttttgccga
     5221 ttgaaaaatt ttttattttt ttgttgttct tctgctcttg gtgcatctct gtatatctgt
     5281 tgctcgtatt ggttcaaatg ttcaaatgaa tgctgctctc tttcataaat acgggtagca
     5341 tcttcctcat tttccatacg cgttcgtttt cgttctcttc tagcaaaaat actccccgta
     5401 tcccgcttca gaggtttcgc cttcttatgg atgtccttat agcgatccat ccgactcatt
     5461 gtggttcctc ctcgtttctt actgataaat aaaggatacg ataaccaatc gacaaaagcc
     5521 tatcattttt tgataattta ataaaaaata acgaaataaa tgcttcgata cataaaggat
     5581 tacttttctt gagaccgttt atgcgagaat aaaagatctt tatctattat ccttttttaa
     5641 tcaaacagct tttcaatcac atggatcggc atcttttcct ttgtccataa ttcaaacgcg
     5701 attgctcctt ggtagatcaa catgccaagt ccattgtatc cttttgctcc tacttttttt
     5761 gcttctttta ataaacgtgt ttctctaggt gcataaatca aatcaaaaac cgctagattc
     5821 tcatgcatca tgcttatatc taagagcggt gactgattga ttttctttcc catcccgata
     5881 tctgttgcat tgactagaag acagctttct ttcaagtctt ttcttaattg atgcgtatcg
     5941 gcaaagtcac gaacttttat cttacaagaa gttcgatagc tgagttgttc gaatgtttct
     6001 tttacttgag cgaatgtttg gttgtttcgt ttataaacta cgatttcctc agcgccgtct
     6061 aacgctgctt gaacagtgac tgcctttgct gcacctccag ctcccagtag agtcattttt
     6121 ttctttttga tcgaaatccc agcttcttgc acggattgca tgaagcccgc accatcagta
     6181 ttatagccaa tcaaacgacc cttttcgtgc acgatcgtat tcactgctcc gatcaattgc
     6241 gcttcctggc ttacctcatc cagataagga ataaccgctg ttttattcgg catcgaaaga
     6301 tttacaccta acatatcaaa agttcgaata ctttcgactg cttgcttcaa attcattgga
     6361 tttacttgga aagctaaata caccgcatcg atgttgtttg cttcaaaagc agtattgtgc
     6421 atcaatgggg ataaactgtg agaagccgga tcagcaaaga gcccagctaa tttggtttgt
     6481 ccagaaatca tcttattcct cctttgttct tacttcaata aaaaaagcca cttagc
//
LOCUS       DAYYHB010000115         6483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000115 DAYYHB010000000
VERSION     DAYYHB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.117"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..905)
                     /locus_tag="ACHA6B_002442"
     CDS             complement(3..905)
                     /locus_tag="ACHA6B_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8949982.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            1091..1660
                     /locus_tag="ACHA6B_002443"
     CDS             1091..1660
                     /locus_tag="ACHA6B_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8949983.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1718..1897
                     /gene="rpmF"
                     /locus_tag="ACHA6B_002444"
     CDS             1718..1897
                     /gene="rpmF"
                     /locus_tag="ACHA6B_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8949984.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            2067..3488
                     /gene="gndA"
                     /locus_tag="ACHA6B_002445"
     CDS             2067..3488
                     /gene="gndA"
                     /locus_tag="ACHA6B_002445"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8949985.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            3681..4367
                     /locus_tag="ACHA6B_002446"
     CDS             3681..4367
                     /locus_tag="ACHA6B_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8949986.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            4364..5869
                     /locus_tag="ACHA6B_002447"
     CDS             4364..5869
                     /locus_tag="ACHA6B_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8949987.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         2210 a         1070 c         1357 g         1846 t
ORIGIN      
        1 ttttacgaag agctgttttt ttgtgctgta acgaattcat ggataacttg gactacttgt
       61 tcttgaagag gcgtgacgat ctgctgtgtt cgttttgcca atgaaatcaa gaagcgaggc
      121 tgaacttcat ctgaaagagg gaggcaaact aaatgatctt caggatggac agcaatctct
      181 gccaaaaaac cgatacccac attttctcgg accatactct ttaaaatcgt caagtcattg
      241 ctttgataaa caacttttgg atgagtatgt gtttgtttag aaagcttagt gaatgctgcg
      301 gggtggacat aatgttcgtt caatagtaca aactgttcat ctgctatatc tttgaaagag
      361 acagtatcct gcttggaaag aggatgtgta ggtggcacga cgatcatgaa acgtttgtcc
      421 aaaagtaact ctgtttctaa atattgatcc acgattggct gtgttgagcc taatacagct
      481 aaatcgatcc tcccttgtcg gatcatcccg tataagtccc gagagcctcc atcggtaatc
      541 tcaatttgat tcatcaattc atgcttgata aagtaacttg acagtttagg aaagtattca
      601 ttgccgataa taggaggcat gccaaaacga agctttttct tttgcaactg agtgatttca
      661 gctcttgcag tagaaatctc ttggagaatg gtttgtgcat ggagaaacag ctgctttcca
      721 gaaggcgtga cgattaattc gtgatgcgat cgatctcttg taataagtac ggtgtcaaac
      781 tctttttcaa gacgttgtaa tgcatatgta atagtaggtt ggctcacacg gaaataatta
      841 gccactttcg taaaattttt ttgtttgacg atttggacat agtattcgat atctttcgtg
      901 ttcatgaaga tcatccgctc ctttaatgga ataatgatta ttgtaaatga ttctgaggat
      961 gatagcaaac agaggctttc taataaagat ttttctgttt ttttatgaac tgcaaggtgt
     1021 caagaaaatt tcattgacaa catgcttttt gccctgtata attgcttttg ttgcttagga
     1081 gtgatgaaaa atgaaatggt ctttattgga attgagaaaa taccaggaga caccgttaac
     1141 ttttcatgag acgctcgact tgaaagccgc tctcatgaag cgagacaatt taatacttga
     1201 tgttgcgcca gtggaagtcg aaggtttggt ctcagttgat aagaaaggct atatcgttca
     1261 ttacactgtg cagacgactt tgacagttcc ttcaacacgt tcactagaac ctgttgaact
     1321 gcccatggat ttttctgtag acgaagtatt catgacagaa gaacaattcc aaagtagaga
     1381 tgaacaaatg tctgcggatg agattttgct aattgaaggt ccattattga atttagcaga
     1441 gtcgattgaa gacaacatct tattggcgat tccaatgcgg gtattgacaa aagaagagga
     1501 agcagcaaca gaacttccaa aaggcaatga ttgggaagtt gtttccgaag aagaatacca
     1561 gcgcagaaaa atggaagaag ccgagaataa aattgatcct cgtcttgcaa aactatctga
     1621 actttttgat tctaaatcaa aagacgacga taacaagtaa gattggaata tattgtgtct
     1681 aatttagata caagacttta caaggaggtg tagcaaaatg gcagtaccag ctagaagaac
     1741 atcaaaagct aaaaaagcaa aacgtcgtac acactacaaa ttgacaatca aagggttaaa
     1801 cgaatgtcca aactgtggtg aaatgaaaaa aagccatcac gtatgtgcaa attgtggtta
     1861 ctatgatggt aaggacgtaa tgtcaaaaga agcttaatac attttaatta gaaaatgtaa
     1921 aacctcaagg ctaaccatgg agccttgagg tttatttttt tgtagtcggt aaataaaaaa
     1981 tatgttgttc tttagtttgc aatgtagccg tgttaagcta taatgtaaga gattagggag
     2041 aaatcccgta tgtaggagga gttcatatgt caaaacaaca aatcggcgtt gtcggtatgg
     2101 ccgttatggg taaaaatcta gccttgaaca tagaaagccg cggctattca gtggctttat
     2161 tcaatcgtac aggggctaaa acaaccgctg tagtagaaga acacccagat aaaaatttca
     2221 aagcaacata tacaatcgaa gaatttgtag aatctatcga gaaaccacgt cggatcttgc
     2281 taatggtcaa agcaggaccg gcaacagatg caacgatcca agagttgctt cctcaccttg
     2341 ataaaggcga tattttgatt gacggaggaa atacattctt taaagatacg atgcgtcgta
     2401 atgaagagtt agccaactct ggcatcaact ttatcggtac aggcgtttcc ggtggtgaag
     2461 aaggagcttt aaaaggacct tcaatcatgc ctggtggaca aaaagaagcc tatgcgctag
     2521 ttgcaccaat cttagagcaa atttctgcaa aagcagaaga cgaagcacca tgtgtcacat
     2581 atattggccc aaatggagcc ggacattatg tgaaaatggt acacaacggt attgagtatg
     2641 gagatatgca actgattgct gaatcttatg acttaatgaa gaatatttta aacctttcag
     2701 tagaagaaat ggctgatatc tttaaagaat ggaatcaagg cgaattagac agctatttga
     2761 tagaaatcac tgctgacatt ctgacacgta aagacgatga aggaacaggt aaaccagtag
     2821 tagatgtgat ccttgatgca gctgggaata aaggaacagg taaatggaca agccaaagcg
     2881 cgcttgactt aggtgtgcca cttccattga tcactgaatc tgtatttgcc cgctatattt
     2941 ctgcttataa agaagaacgc gtacaagcaa gcaagatttt atctcgtacg aacgattttg
     3001 agttcacggg agacaaaaaa gaattggtcg aaaaaatccg agaagcgttg tatttcagta
     3061 aaatcatgag ttatgcccaa ggatttgctc aattacgtgt cgcttcaaaa gaatttgatt
     3121 gggacttgcc atttggtgaa atcgcaaaaa tctggcgcgc tggatgtatc atccgagctc
     3181 gattcttgca aaaaattacg gatgcctatg acaaaaatcc tgagatcgaa aatttattgc
     3241 tagatgatta cttcgttgaa atcacgaaaa aatatcagca atcagtcaga gacgtcgttg
     3301 ccttggctgt ccaagctggc gtacctgtac cgactttctc atcagccatc gcttacttcg
     3361 attcatacag agcagaacgt ctgcctgcaa atataatcca agcccaacgc gattactttg
     3421 gcgcacatac ctatgaacgt gtcgacaaag aaggaatctt ccattactca tggtaccatg
     3481 aagaataata gtctttcggc tgttaggaaa tgagaaaata aaaagaggac gaaagagctg
     3541 aagtcgattg ttatgagtag acttcagctc ttttttaaaa ttgctatcca tgataaaatg
     3601 actatagatt tttaagcata ttatcggtat aatgatagag tttttaaaaa gccagtagaa
     3661 tagaaaggat aaaataataa atgagcaata ttctaatcat tgaagatgaa aagaacttgg
     3721 cacgatttgt cgagctagag ttgaagcatg aagggtataa cgcagaagtc cactacaacg
     3781 gccgaactgg tttagatgcg gcattaaata atgagtggga tgccatattg ttggatctga
     3841 tgctcccaga attgaacggt ttagaagtct gcagacgtgt acgccaagtc aaaaatacac
     3901 caatcattat gatgactgca agagattctg tgatcgatcg tgtatctgga cttgaccacg
     3961 gagcagatga ctacatcgtc aaaccatttg ctatcgaaga attattagca cgtcttcgtg
     4021 cattgttgcg tcgaatcgat attgaaggcg ataaaaatgt ggctaaacaa actactatta
     4081 cttatcgcga tttgactatc gaaaaagaaa accgtgtcgt acgtcgcgga aatgaagtaa
     4141 ttgaactaac aaaaagagaa tacgagttgc tgcttacttt aatggaaaac gtgaacgtgg
     4201 tattagcacg tgacgtttta ctaaataaag tgtggggata cgaaacagaa gttgaaacaa
     4261 acgtagtgga cgtatatatt cgttatcttc gcaacaaaat tgacgttccc ggggaggaaa
     4321 gctatatcca aactgtacgc ggaacaggtt atgtgatgcg ctcgtgataa agccgaaaaa
     4381 atcggaaaaa agagaactga aagggccctc cttgacaatc aagtgggcct ttgcgagttc
     4441 tttcttcata ttcgttgtgt ttacaatttt cgctgtcatt acctataaat catctatcaa
     4501 tctgattgtg gcaaaagaaa gaaacaatgt agagcgtacg atttcagaag tagcttctcg
     4561 tttggctaac tcggatgaag aactgacatt agtaaatacg tatcgtaatc tgacgaaagc
     4621 gacaaatgac ggagatgagg tcgtagatca tacttcactg gtcgaaggaa acttaatgaa
     4681 gatcgattcc tttatagctg agctagggca gccatcaatg gatctttatg tttatgattt
     4741 ggacgagaac ttgattttta aaacccatga aaacagtcga aacctgattc agactacacg
     4801 taaagttcca acgatcgtta cactagaagg aaaaactggc tttttatcta tccagcctat
     4861 atattctaaa ggtacaagag aaaagatcgg ctatgcgcaa agcttctatg aattatcttc
     4921 tttctatgat attcgaaata agctattatt gactcttatt gttttagaag ttgtttcttt
     4981 gatcttaagt agtattttag gtttcttttt atcttcttat ttcttaaaac cactcaaggt
     5041 actcagagat acaatggata ctatacgtaa agatccgcaa tcagatatcc acatgcctga
     5101 aatcgacaca aatgatgaat tggcagattt agctgaaata ttcaatgaga tgcttgaccg
     5161 tatgcgtttg tatattgaac aacaagagca attcgtggaa gacgtatctc atgagttaag
     5221 gacacctgtt gcaattattg aagggcatct tagtctgcta aatcgttggg ggaaagatga
     5281 tcctgagata ttagaagaat cattaagcgc cagcatgcaa gaaattagcc gtatgaaaag
     5341 cttagttcaa gagatgctag acttatcaag agcagaacaa gtagatgttc attatgcgaa
     5401 tgaaacaact aaaggaaaag aagtcaccta tcaagtttat aataatttca agatattata
     5461 tccagactat gtgatcactt tagatgatga tttgacaaaa gaagtgaccc tgcaaattta
     5521 tcgtaatcac tttgaacaat taatcattat catattggat aacgcagtaa agtattcaac
     5581 tacacgaaaa gaagtccata tctctatttc ttccactctg aatgaatttg agatcgcgat
     5641 tcaggacttt ggcgaaggaa tacccaaaga agaccttaat aagatcttca atcgttttta
     5701 tcgagtggat aaagcaagag ctagaacgaa gggtggaaat ggtctggggt tgtctatcgc
     5761 taaacaactg gttgaaaact ataaaggacg tatcttagca gaaagtgtag tagggcaagg
     5821 gacaattttt agaatcttta ttcctatcgc aaaggcagaa gacaagtaaa gcatgtgctt
     5881 tacttgtttt tttatatatc caacaaagaa taaggtacaa aatacaagca agttagaaat
     5941 tcagggatgt cattataaaa aaaactagcg aacaattaaa tagaataaca tccgttaatt
     6001 aaacgtaagt agcttttaga tgaacgaaaa aacaatgaaa tgctgtaata ttcgtgttta
     6061 aaaataataa ataaaaatag ggaattttct attgcaattt tataaagaga ttgctatatt
     6121 attatttgtt ctaaatgatt cctttaaggt gtcactaaaa attataaaaa atagggttga
     6181 cgcacttttg atgtcatgtt ataataacga agtcgtttgg atgaggtagc taataaagct
     6241 tgattttatc gaaaaaaact tcgaaaaagt tgttgacaaa taacaataaa gatgatatga
     6301 taatcaagtc gctgctaatg agtgacaaag acgtttagaa aaaataaaca aagaagttgt
     6361 tgacattctc ttctgaatct gataagatta aagagttgtt gaaacaaaaa gattagacct
     6421 ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga
     6481 caa
//
LOCUS       DAYYHB010000116         6330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000116 DAYYHB010000000
VERSION     DAYYHB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.118"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..882)
                     /gene="truA"
                     /locus_tag="ACHA6B_002448"
     CDS             complement(142..882)
                     /gene="truA"
                     /locus_tag="ACHA6B_002448"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8949988.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1073..1708)
                     /locus_tag="ACHA6B_002449"
     CDS             complement(1073..1708)
                     /locus_tag="ACHA6B_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8949989.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1855..3075)
                     /gene="sstT"
                     /locus_tag="ACHA6B_002450"
     CDS             complement(1855..3075)
                     /gene="sstT"
                     /locus_tag="ACHA6B_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8949990.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            3294..4292
                     /locus_tag="ACHA6B_002451"
     CDS             3294..4292
                     /locus_tag="ACHA6B_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949991.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4488..6323
                     /locus_tag="ACHA6B_002452"
     CDS             4488..6323
                     /locus_tag="ACHA6B_002452"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8949992.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
BASE COUNT         2096 a         1226 c         1013 g         1995 t
ORIGIN      
        1 tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata
       61 tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt
      121 tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc
      181 agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc
      241 tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt
      301 atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga
      361 taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat
      421 gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc
      481 ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac
      541 ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc
      601 ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc
      661 tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc
      721 agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac
      781 tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata
      841 tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg
      901 tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc
      961 ttctctaaga cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa
     1021 aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt
     1081 tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt
     1141 ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc
     1201 cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt
     1261 ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac
     1321 tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac gaagattcat
     1381 gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg
     1441 ctgctgtaac tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag
     1501 gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta
     1561 caggactgga aacctctcca aatacactga tcacgctctt catttgatct aattcaggat
     1621 aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga
     1681 aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt
     1741 taattgttat tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat
     1801 ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg
     1861 ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc
     1921 gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat
     1981 tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt
     2041 agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc
     2101 tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg
     2161 tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc
     2221 accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa
     2281 tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa
     2341 gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt
     2401 aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat
     2461 caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga
     2521 ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg
     2581 tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa
     2641 agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga
     2701 ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg
     2761 cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac
     2821 aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt
     2881 cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac
     2941 cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa
     3001 tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg
     3061 ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa
     3121 caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca
     3181 atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag
     3241 atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt
     3301 tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct
     3361 tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag
     3421 tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa
     3481 cttccgttgt tattggtgca aatttgatac cactttacat gggttaccaa tatcaaaaaa
     3541 tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca
     3601 cactagtttg caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt
     3661 ggcttatcct aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc
     3721 ttttttgtag tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta
     3781 aatatctact attattttta acaattggat tagttggctg ctctactcta tttaataaag
     3841 acccgtttgc attacagaat ggacagagta ttttatggat tttttattta tttttaatgg
     3901 gttatggttt gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat
     3961 ttatcagctt gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca
     4021 tcagaggaaa tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt
     4081 attatacttt ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag
     4141 gaagaggaaa ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt
     4201 tagtttctta tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat
     4261 ggctgataaa tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta
     4321 tctttttatt ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt
     4381 ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc
     4441 ataaaagtta ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg
     4501 ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc
     4561 agcgacaagc acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat
     4621 tgttgatttc caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc
     4681 tcactatttc gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt
     4741 taaaaatgct gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg
     4801 tgggcggatt gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac
     4861 aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat
     4921 taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt
     4981 ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg
     5041 gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct
     5101 attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca
     5161 accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct
     5221 tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca
     5281 aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa
     5341 cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt
     5401 tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc
     5461 tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag
     5521 tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat aaattaactt
     5581 atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct
     5641 tgaaagcttt acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa
     5701 accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg
     5761 tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata
     5821 cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt
     5881 tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag
     5941 aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta
     6001 cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga
     6061 atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat
     6121 taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta
     6181 cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag
     6241 aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg
     6301 cgacacaaaa aataaaagac taacaaataa
//
LOCUS       DAYYHB010000117         6314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000117 DAYYHB010000000
VERSION     DAYYHB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.119"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA6B_002453"
     CDS             complement(99..1229)
                     /locus_tag="ACHA6B_002453"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8949993.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA6B_002454"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA6B_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8949994.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA6B_002455"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA6B_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8949995.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACHA6B_002456"
     CDS             complement(3485..4549)
                     /locus_tag="ACHA6B_002456"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8949996.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA6B_002457"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA6B_002457"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949997.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA6B_002458"
     CDS             4880..5692
                     /locus_tag="ACHA6B_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8949998.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA6B_002459"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA6B_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8949999.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1875 a         1298 c          996 g         2145 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttct
//
LOCUS       DAYYHB010000118         6309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000118 DAYYHB010000000
VERSION     DAYYHB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.120"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1553)
                     /locus_tag="ACHA6B_002460"
     CDS             complement(75..1553)
                     /locus_tag="ACHA6B_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950000.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1851..2786)
                     /gene="murB"
                     /locus_tag="ACHA6B_002461"
     CDS             complement(1851..2786)
                     /gene="murB"
                     /locus_tag="ACHA6B_002461"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8950001.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2859..3779)
                     /locus_tag="ACHA6B_002462"
     CDS             complement(2859..3779)
                     /locus_tag="ACHA6B_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8950002.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3892..4644
                     /locus_tag="ACHA6B_002463"
     CDS             3892..4644
                     /locus_tag="ACHA6B_002463"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8950003.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5508..6041
                     /locus_tag="ACHA6B_002464"
     CDS             5508..6041
                     /locus_tag="ACHA6B_002464"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8950004.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1903 a         1203 c         1049 g         2154 t
ORIGIN      
        1 tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg agacaaagct
       61 gccatccata aatactagct gattttttca gttaaatgtc tatttttttc tctgatgatg
      121 gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat tatctgttct
      181 ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga agtgattcga
      241 ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat gtaatcggta
      301 taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa atatgatacc
      361 ggacaaatac tgaccaataa aaagagataa tcataaaata agcgaatcat gaaacgttca
      421 tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc tgtactgaat
      481 attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct agtcatctca
      541 aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag ttcttttcgt
      601 atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc aatcaattcc
      661 actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc aagtccaaat
      721 gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata agaaatctca
      781 tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc cttgatgatc
      841 ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc gattggttga
      901 ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat tcgttttgtt
      961 gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt tgcttcattc
     1021 atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata taccccaaat
     1081 aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa ctttatttga
     1141 tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga tatgcttaaa
     1201 aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc cgttaacagt
     1261 cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc cgtcaaattc
     1321 agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt tgttttgaat
     1381 tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt agacaaacct
     1441 aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc taaatatcta
     1501 gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt catatcctac
     1561 acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca tcttttcacc
     1621 tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca ttttttatct
     1681 tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct atattttttg
     1741 ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca caaaaaagag
     1801 caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta ttaattggct
     1861 aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac ttctgtttct
     1921 aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa ttcaatataa
     1981 tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc agagacttgt
     2041 gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt
     2101 cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg ttttgattga
     2161 cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc tgtttgcaaa
     2221 ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg acgataagaa
     2281 aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa aagatccact
     2341 tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata aaccgctcca
     2401 ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc tgccaaagct
     2461 gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca
     2521 tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc atcttgaacg
     2581 atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg gcaataatcg
     2641 atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc tggtccacct
     2701 gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat cgtggctaag
     2761 ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca aacgtcctca
     2821 ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg acggtagtga
     2881 tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg tttaagcaat
     2941 tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat tggctcaaac
     3001 ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt tccatcagca
     3061 tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt acttgttact
     3121 tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc cgtttgattt
     3181 tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct tatctggtat
     3241 tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag atgtaaaaac
     3301 aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg
     3361 atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa gcgccggttg
     3421 aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc acgaacttca
     3481 tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc tagacatttt
     3541 ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc gattttttct
     3601 tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt ttgaattctt
     3661 tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca
     3721 taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc aatcttcatc
     3781 tactttcctc ctggatcatt tagttacttt catcttacac catttttttc taaaatgagg
     3841 tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt attgaaattt
     3901 atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt agaagttttt
     3961 catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc aggacaaatt
     4021 gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa aggctattcc
     4081 ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat gggtatcgat
     4141 atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt tcttgtaacc
     4201 tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt agaatgggaa
     4261 gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg
     4321 gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat
     4381 gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa cgggtttatc
     4441 gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg gaactaccga
     4501 ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac gagcaaagat
     4561 ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag tgatcattgt
     4621 ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta
     4681 agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata ataccgagaa
     4741 actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat
     4801 aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct tattaccgca
     4861 gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac tgcatcaagt
     4921 aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg gcttattacc
     4981 gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg cactgcatca
     5041 agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt tggacttatt
     5101 accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca gcgtcctgac
     5161 ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt ttttgagcta
     5221 ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac
     5281 tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat aaatttgact
     5341 taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga gttcaaaaac
     5401 tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa tttatttgct
     5461 tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg aattttgttg
     5521 ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt gcgctagtca
     5581 tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct gagactcctt
     5641 ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc aatgcgccga
     5701 aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt ttagtcgttg
     5761 cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat tatcatcttg
     5821 aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat
     5881 ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta gatcatcacc
     5941 atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat
     6001 tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact tatgaaacac
     6061 acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata
     6121 gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg agaacaccgt
     6181 ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
     6241 atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag cttttggaca
     6301 ccgtttacc
//
LOCUS       DAYYHB010000119         6066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000119 DAYYHB010000000
VERSION     DAYYHB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.121"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACHA6B_002465"
     CDS             complement(254..541)
                     /locus_tag="ACHA6B_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8950005.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACHA6B_002466"
     CDS             complement(561..785)
                     /locus_tag="ACHA6B_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8950006.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1443)
                     /locus_tag="ACHA6B_002467"
     CDS             complement(1240..1443)
                     /locus_tag="ACHA6B_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950007.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACHA6B_002468"
     CDS             complement(1425..1958)
                     /locus_tag="ACHA6B_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8950008.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACHA6B_002469"
     CDS             complement(1979..2923)
                     /locus_tag="ACHA6B_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8950009.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACHA6B_002470"
     CDS             3560..3646
                     /locus_tag="ACHA6B_002470"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8950010.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACHA6B_002471"
     CDS             complement(3767..4456)
                     /locus_tag="ACHA6B_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950011.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACHA6B_002472"
     CDS             complement(4463..4741)
                     /locus_tag="ACHA6B_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950012.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACHA6B_002473"
     CDS             complement(4701..5615)
                     /locus_tag="ACHA6B_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8950013.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACHA6B_002474"
     CDS             complement(5597..5977)
                     /locus_tag="ACHA6B_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8950014.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1120 c         1021 g         2319 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtcgatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAYYHB010000120         6063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000120 DAYYHB010000000
VERSION     DAYYHB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.122"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..128
                     /locus_tag="ACHA6B_002475"
     CDS             <1..128
                     /locus_tag="ACHA6B_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950015.1"
                     /translation="QTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(214..1308)
                     /locus_tag="ACHA6B_002476"
     CDS             complement(214..1308)
                     /locus_tag="ACHA6B_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8950016.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1497..2258
                     /locus_tag="ACHA6B_002477"
     CDS             1497..2258
                     /locus_tag="ACHA6B_002477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8950017.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2373..2813)
                     /gene="fabZ"
                     /locus_tag="ACHA6B_002478"
     CDS             complement(2373..2813)
                     /gene="fabZ"
                     /locus_tag="ACHA6B_002478"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8950018.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3033..3788
                     /gene="fabI"
                     /locus_tag="ACHA6B_002479"
     CDS             3033..3788
                     /gene="fabI"
                     /locus_tag="ACHA6B_002479"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8950019.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3898..4950)
                     /locus_tag="ACHA6B_002480"
     CDS             complement(3898..4950)
                     /locus_tag="ACHA6B_002480"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8950020.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            5076..5996
                     /locus_tag="ACHA6B_002481"
     CDS             5076..5996
                     /locus_tag="ACHA6B_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8950021.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1860 a         1205 c         1079 g         1919 t
ORIGIN      
        1 atcagacatt tatttccagt gtcagcaatc aacgtaatca tattccaaga aaatcattga
       61 attacagaac accaatcgaa atatttttga gctatgtaca agaagcattt tattctagct
      121 taatttgaca aatcataacc tataaaaaaa caaaataaaa aagcagaaat aactggctat
      181 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
      241 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      301 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      361 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      421 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      481 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      541 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      601 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      661 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      721 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      781 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      841 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      901 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      961 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
     1021 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
     1081 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
     1141 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1201 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1261 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1321 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1381 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1441 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1501 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1561 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1621 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1681 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1741 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1801 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1861 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1921 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1981 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     2041 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     2101 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     2161 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2221 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2281 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2341 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2401 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2461 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2521 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2581 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2641 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2701 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2761 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2821 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagtat catattccta
     2881 aatatttagc aatctttttt taatttaaga aaaaatagtt tgacaatcaa aacattttga
     2941 ctaataatta agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat
     3001 caaattttta tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg
     3061 tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga
     3121 aacaaggggc agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca
     3181 aattggtcgg cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg
     3241 aaaaagcaat gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag
     3301 ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct
     3361 atcaattagc tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac
     3421 cgtttcttgc accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa
     3481 tcccgaatta taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt
     3541 tggcagctga gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca
     3601 aaacattagc tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc
     3661 gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa
     3721 tcagcccact ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc
     3781 tttcataaac ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt
     3841 ttaaagtctc ttcctgattt tttcttttta cgtttttttc aactcattac tttgaaatta
     3901 ttccacgact tcaatccagc cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa
     3961 ttcatcatac aaccgttttg ttactggtcc tacttccgtt tcactataaa atacatgaag
     4021 attgttattg tgataaatcc caccgattgg agaaataaca gccgctgtac cacatgcacc
     4081 agcttctgca aattgatcta gatcatcaat gaaaatatct ccttcaattg cttgcagtcc
     4141 tagtcgttct tcagctaact gcaacaagga atatttcgtg atacttggta agatagaggg
     4201 cgattgcgga gtgatgaatt ttccgtcttt tgtaattcca aagaaatttg ctgcacctac
     4261 ttcttcgatt ttcgtatgtg tggctggatc aagataaaca caatctgagt aatccagttc
     4321 ttttgcttct gttcctggaa gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc
     4381 agtcccctta ggagcagcac gatcgtaatc agatacaata aaattcgttg gttttagtcc
     4441 acctttgaaa tacgctccta ctggcgtaca gaagacgaca aataaatatt ctggagctgg
     4501 atggacacca atgttttcac cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc
     4561 tgttccatag ggcggcacaa aatcttcatt tgcttttacc acttgcttga cagcatcaac
     4621 gaacatcttt tcagggacca ttggcatcag caatcgtcta gcactttgat tcatccgtcg
     4681 actattttgt tcaggtcgaa agagattgac cttgccgtct tttcgacgat aagcttttag
     4741 accttcaaaa cattgctgac cataatgaag cgctggagag ccttcatgaa tcgtcaggta
     4801 attatcttct gtcaattctc cttcttccca ttgtccgtct ttccatttgg caataaaacg
     4861 ccaaggagtt ttgatataag aaaaacctag attgttccaa tcgatttgca tatacttacc
     4921 accttcgtta ttttctccac attttatcat tatccatatt atttgtcatt ttattttcag
     4981 cttttttcct tcattatagt tatttttgct atttcctttt ttgaaaactt cttggtacaa
     5041 tacagaaaga atcaaatttt agaaaagagg attttatgtt ttcattatta gcaactgccg
     5101 gtacgacaga ctcttctggc gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt
     5161 ttcagcggtt ttgggacggg attgattggg acgccatttt ggctgccttt atccaaaaaa
     5221 gtttatatct gatttttttg atcgtccttt tttggattct tcaccgtgtg ggaaaatatc
     5281 tgattgataa gtcttttaat cagtattcta aaaaagccat tctaaacgag agccgcttaa
     5341 agactttaca tagtctttta aatactattt ttcattatac ccttggtttc ttttttatct
     5401 atgcaatttt atctgctatc ggtgtgccga tcggatcact tttggctggt gccggaattg
     5461 caggtgtggc aatcggtctt ggtgcccaag ggttcatgag tgatgttatt accggatttt
     5521 ttattatcat ggaacaacag atggatgttg gggactacat caagctagca aacttaacga
     5581 ttgaaggaac cgtcgtatct gttggtatcc gtacccttca gctgaaagca acggatggta
     5641 ctgtccattt catcccgaat cgaaacatca ccacgatcag caatctctct cgtgcaaata
     5701 tgcaagtact tttagatatt cggattgttc ctgaagaagg atatgacaaa atctatgaga
     5761 tcattgatcg agtaaatcgt acattagctg aaaaatacca agaagagctc caaactgaac
     5821 cttctatctt tggattagtg gatcttggaa acagcaattt tgcgattcgt acggtctgct
     5881 acgcattgaa cggaaaacaa tatgccttaa aagaagagtt cttgagctcc tatgtcaaag
     5941 aattgacagc ttctggtttc acgattccga acagtccaat tgctgtcgga aaataatttg
     6001 gacaaacgat gcaacgaatc tcactaaaaa gagcgtggga caaaagtaaa gaacactttt
     6061 gtc
//
LOCUS       DAYYHB010000121         6005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000121 DAYYHB010000000
VERSION     DAYYHB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.124"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..352)
                     /locus_tag="ACHA6B_002482"
     CDS             complement(50..352)
                     /locus_tag="ACHA6B_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8950022.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(572..938)
                     /gene="ssrA"
                     /locus_tag="ACHA6B_002483"
     tmRNA           complement(572..938)
                     /gene="ssrA"
                     /locus_tag="ACHA6B_002483"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1109..1882)
                     /locus_tag="ACHA6B_002484"
     CDS             complement(1109..1882)
                     /locus_tag="ACHA6B_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8950023.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2026..2376
                     /locus_tag="ACHA6B_002485"
     CDS             2026..2376
                     /locus_tag="ACHA6B_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8950024.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2357..3073
                     /locus_tag="ACHA6B_002486"
     CDS             2357..3073
                     /locus_tag="ACHA6B_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8950025.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3073..4521
                     /locus_tag="ACHA6B_002487"
     CDS             3073..4521
                     /locus_tag="ACHA6B_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8950026.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4591..5100)
                     /locus_tag="ACHA6B_002488"
     CDS             complement(4591..5100)
                     /locus_tag="ACHA6B_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950027.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5090..5815)
                     /locus_tag="ACHA6B_002489"
     CDS             complement(5090..5815)
                     /locus_tag="ACHA6B_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950028.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2044 a         1047 c         1169 g         1745 t
ORIGIN      
        1 agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt taatctttat
       61 acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga tcgctccgtg
      121 cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc attctggttt
      181 ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt aataagctat
      241 cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc
      301 tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac ataatgttca
      361 cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc attgataata
      421 tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa tgccattttc
      481 attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa aataaggatt
      541 taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt cgaacccgcg
      601 tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa ggattcactt
      661 tacagcttgc cgagtgacag acattctgta tcgctagtct gatgttctct ttttcaactt
      721 acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga gcacatgggc
      781 gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga gttgttttcg
      841 tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat
      901 tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa gggttacgtg
      961 tatttttcat acaaaattag tatagcatat attgggataa attcaataaa aaagcgaatc
     1021 atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc gattttctta
     1081 agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc aatgcgtgga
     1141 caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt aatccgtcat
     1201 aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac
     1261 catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc agacgtttga
     1321 cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga ccattccgat
     1381 aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga gataagatca
     1441 gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga acatctgaat
     1501 gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag attttctgat
     1561 cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt ttttgacatt
     1621 cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg aaagaaccat
     1681 tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt tctgcttctg
     1741 tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc atagcttttg
     1801 ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag
     1861 tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa gtttatctta
     1921 acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat tggttcagtc
     1981 aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat ttttttcagt
     2041 attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc aaaaggaaat
     2101 ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg
     2161 gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc attgatcggc
     2221 tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct tctgctagta
     2281 tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata tgacctggaa
     2341 actggaggga acgataatga atattttaat gattgaagat aatcaatctg tttctgaaat
     2401 gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg atggaaaaga
     2461 agggctcgat gcctttttag aaaacccaca acattgggat atgattacac tagacctgaa
     2521 tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt caagtactgt
     2581 gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg gcttagagat
     2641 gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg ctcgaatgaa
     2701 agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag tttcagatga
     2761 tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag aagcgtatct
     2821 ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt ttactttagc
     2881 aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct gggattatca
     2941 gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac aaaaaatcga
     3001 aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat tcgatgattc
     3061 tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg aatggtcgcg
     3121 ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac gattgaagaa
     3181 aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac gcaaacctat
     3241 gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt gctgctgaca
     3301 gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc
     3361 atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca atggaaaaat
     3421 ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg acaagcacag
     3481 gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg agcacttgta
     3541 gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact gaacctgttc
     3601 aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt tgcagcattc
     3661 caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt
     3721 gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga tgatttcaat
     3781 aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga aaatcgtcga
     3841 agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac aatcaacgga
     3901 cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa
     3961 ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt ggattatgaa
     4021 aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac agaaacattg
     4081 aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac attgacattg
     4141 aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca aatcatggtc
     4201 aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat tactttagaa
     4261 aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat gtctgaacaa
     4321 caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa aaatacaaaa
     4381 tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt gcataaagga
     4441 aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac
     4501 gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc gatcttctat
     4561 accgcttgtt tttactataa aatgattgat ttagcccgcg ggctctaaac gatcaatata
     4621 aggactttga gcaatagtag catcatgagt aaagagtaag ataccttgat tttcttcatg
     4681 agctaaataa gaaagcaagt ggagtagctc ttggatctcc aatacactaa gttcatcaaa
     4741 tgtatcttca ataacaatat tgtttcgctt ggaaagcaag gcatgaagta attgcagctt
     4801 tactttttca aaagacgtca actcagctgc cgcacaatta agcaaggaag gagagatatc
     4861 aagtttttct tgataggcca aacgatcttg tttgatttct ttttccggta catccaacag
     4921 gacatggtct ttaagattca gataaggaaa catcggccaa tttttttgga tgatcgcaaa
     4981 attatccagt tttgcaacaa gaacctcttt tttcaagtgc tccaatattt tatgctgttt
     5041 ttcgagagga cacaaaataa cggtcaagtg ttcagcatga atcaaatatt caggtttcat
     5101 tacgtccagg cctcctactt tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa
     5161 gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta agtatatctg
     5221 tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg
     5281 taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta gtctttaaga
     5341 caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt acgatccaca
     5401 ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa ataaaaagga
     5461 ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc ttgttttgcc
     5521 tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata
     5581 tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct gtagatgcag
     5641 aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat tgcaaatggt
     5701 aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa caatagagta
     5761 ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt ttcatacttt
     5821 tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc ctttagtgaa
     5881 atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa gaagctgccc
     5941 tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga ggagcagcga
     6001 caata
//
LOCUS       DAYYHB010000122         5830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000122 DAYYHB010000000
VERSION     DAYYHB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.125"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..742
                     /locus_tag="ACHA6B_002490"
     CDS             <1..742
                     /locus_tag="ACHA6B_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8950029.1"
                     /translation="IYSLLSEKEQENFDEYAYFVNTAIDRQSLGKVLGGKMISVVEPY
                     YEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAAHAAVAYLGALKGYKTIQ
                     EAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKTLNRHGNQKLSDEITRVG
                     SSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYRNLADPEAEQLRRMIVND
                     GLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            754..1185
                     /locus_tag="ACHA6B_002491"
     CDS             754..1185
                     /locus_tag="ACHA6B_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8950030.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            1199..2644
                     /locus_tag="ACHA6B_002492"
     CDS             1199..2644
                     /locus_tag="ACHA6B_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFM8950031.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            2930..4555
                     /locus_tag="ACHA6B_002493"
     CDS             2930..4555
                     /locus_tag="ACHA6B_002493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8950032.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            5187..5540
                     /locus_tag="ACHA6B_002494"
     CDS             5187..5540
                     /locus_tag="ACHA6B_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950033.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         2023 a          807 c         1148 g         1852 t
ORIGIN      
        1 aatttattct ttgctgtcag aaaaagaaca agagaatttt gatgaatatg catattttgt
       61 gaatacggca atcgatcgac aatctttagg aaaagttctt ggaggaaaaa tgatctctgt
      121 tgtcgaacct tattatgaat ggataatgga tcgacagcag ttagatccaa ctacatcttt
      181 tgatatgaaa aatgtaaatt tggttgatga tatggagcct tacattgagc gaaagcttta
      241 tatcgttaat gctgctcatg cggcagtagc ttatttaggc gcactgaaag gatataagac
      301 gattcaagaa gcaatccaaa atatggagat tttcaatcta gtcaagcaat ttttaatgga
      361 gaatatagag tactttgtac aaaaatatca tttttctaga gaagaattaa catcttttgt
      421 ggaaaaaaca ttaaaccgtc atgggaatca gaaactctcc gatgaaatta ctcgtgtagg
      481 aagttcccct attcgaaaat taggccctaa tgatcgttta attgctccca tagtaaaact
      541 agattcatat catttacctt acgatgcagg aataaaagta attgctgcgg gatatcatta
      601 tcggaatctt gctgatccag aagctgaaca actacgtcga atgattgtaa atgatgggtt
      661 gaaagcaacg attaaaaaga tttctcgttt ggaaggtaag ctgctgaatg aagttgtcgc
      721 tgcttatgag tcccagattt agaggaggag aatatgctaa aagaacaatt gtcaggaaat
      781 acaatgttta tggatgcgat cgattcatgg caagaggcaa tccgagtaag tgcgcgtcct
      841 ttgataggaa agggaataat agaggaaagc tatgtacaag cgatgataca aaatgtgttg
      901 gataacggaa attatattat attactgcca ctagtagcaa tgcctcatgc gcgtccagaa
      961 tttgggagca aaggggtcgg gatgagcttt ttatgtttag ataagccagt catgttccca
     1021 agtgaagaac cagtaaaatt cttctttacc ttttcttctg attcgccaaa gggacattta
     1081 gacttaatgg ccaatcttgg agaattgctg agtgatgaaa aaatgtatca aaaattattc
     1141 gatgtgaaga cagaaaaagc attgttagat ttgataaaga agtaaaggag agaagaaaat
     1201 ggaggtagca aacaaaaaaa aacgagtctc catgcgagct aaagttcaag cgattggcgg
     1261 tttcttaaca aatatggtat tgccaaatat aggcgcattt attgcatggg gaattgttac
     1321 tgcattattc attccaactg ggtggttccc aaatgaatct ctgaatgaac tcgtaagtcc
     1381 gacagtcagc tatctattgc cgttactact tgcctatact ggtggtcgga tggttggtgg
     1441 tacacgtgga gctgttttag gtacaatcgg aacaataggt ttgattattg gtgctgacat
     1501 accaatgttc atgggagcaa tgattatggg gccacttggt gggtggatga tgaaaaaaac
     1561 agatgatttt ttagatggga aagttcctgc tggttttgaa atggtcgtca ataatttttc
     1621 tataggaatt attggtttca tactgatgtg cctttcttat ctggtagttg gaccaataat
     1681 ccaatcagct aatcaatttg taacgagtgc aattgaagca ttagtttcaa ctggtttttt
     1741 accgatactg tccttaatca acgaaccggc aaaagtctta tttttaaata atgttattga
     1801 ccaaggtgtt tattatccat taggtatgca acaaacatta gaggcaggca gatcaattta
     1861 tttcatggta gcatcaaatc ctggccctgg cttaggacta ttgttagctt attctttatt
     1921 tggtaaaaag gcagcaaaac agacagcacc aggtgcaatc atcatccatt ttttcggcgg
     1981 aatacatgag atttattttc catatgtatt aatgaaacca ctcactatat tatctatgat
     2041 tgctggtgga atgacaggaa tcatcacttt tgaattattt gatgcgggcc tggtagcagg
     2101 tccaagtcca ggttctattt tttcttattt agctctaacg cctcggggaa attttttggg
     2161 tgtcatggcg ggagtggtca cagcaacact ggtttccttt tttgtaacat cagtaatttt
     2221 aaaagcagat aaatcaacag atgaagacag aaattttgaa ctatcagttc aaaaatcgaa
     2281 aacgatgaaa tcagaaggaa gaaaattatt aaaagaagaa aatgctagag aaatatgtac
     2341 cgagaaaaaa gagatgaata aaatttcctt tgcttgtgat gcaggaatgg gaagcagcgc
     2401 aatgggcgca acaatattca agaaaaaatt gcaaaaagca ggtattactg atgttgagat
     2461 aaaaaattat cgaattgaag aggtaccaga agatagtgat ttggtgatcg tacatcaaga
     2521 tttggaggaa cggactagaa aagcacatcc taacatgaaa attgtgacat taaagaatta
     2581 tttgcaagat ccagtaatca atggattagc agaagaaatc attggattaa aaacagaaga
     2641 ctaatttatg ggaatttttg attaaccttt attaaaatta ttttgcctaa tataaaaagg
     2701 ttgtgacatg gcttgtgtcg caaccttttt ttgtcgcaca aataattaat cggatatttt
     2761 atgttcatat tttttgtgat caacgagaac tgatggggca aagatacatt ttagatactt
     2821 ttaactgtta atttacaaaa caaggtgaaa cgaatagaaa aatgaaaaaa tcgatacgga
     2881 agaatatgtt tgattaaaac atgcatgaag agaaggaggt tgatgataca tgaaaaaaat
     2941 atattcaaag gaaatcgatt gttttagagg cattgctgtt ttagcagtga tattttatca
     3001 tttgccaaat agtttgctgc caggcggatt gcaaggagta acgatctttt ttgtggtttc
     3061 tggtttttta atggctaaaa atacgataag agatgaaaaa aacagacaat tcagtttttt
     3121 acatttttac tataagcgta tcaaacgtat ttatccattt ttactgtttt ctttggtcgt
     3181 attcattttc atactaggga ttagcaacat tcgattgata ggaaacattc gtagtgagct
     3241 acccagtttg atttttggct ataacaattg gtggcagctg aatcaaggag tatcttattt
     3301 tgactcttac tttaatgctt ctttatttaa acattattgg tcgctttcag ttgaactaca
     3361 attctatttg atatggccat ttttgtttat tgtcattaag cgtatgagaa gaaaacaagt
     3421 gttttactta atctatacat tgatttttgt ttctattctt tttagtttgt ttttaccttc
     3481 aactaaggca tattatcata cgatagctaa actttttcca tttttattag gagtttgggg
     3541 gtattttaat cgaataacta ttggccgttt ctttgaacaa aacactttta gtaaaatctg
     3601 gcttttatta ctagctagtc tttgtctgat tctgttcccg atttttcctt atacactaaa
     3661 tgaactactg atcagtattt gttttgctct attgttagcc agtgtcgatg atatgaacat
     3721 tgcaaaaaag accaaaatcg ctagtacaaa actttctttt gtaggaaaaa tcagttatga
     3781 actttatttg gtgcatttcc cagtattgat tgctctgaca aatctattca aacatcacca
     3841 ttggaaatat ttggatatcg caggtctatt cactgtgttt ttatgtacat tactcatgat
     3901 tacttgcttt agaaaaggag tgcaaaagat gagatcaccc aaaatttgga cagctatggc
     3961 actaatgata atcggagttt ctgtactggt attacgtgct cctgccagtc gactgaccgc
     4021 tgaccaaaat cagttgcaaa gtttattaaa agaaaactct agtaaagcaa atgcaggaga
     4081 aaatctgttg tcacaagaga agaaaatatt atttatcggt gattctgtta tgctgggggc
     4141 gtatcaggaa ctaaaggaga catttaaaaa agaagcattg gtagatgcaa aagaatcaag
     4201 acaaatcact gcgttaccag aaatcttaaa ggagcataaa gatttagttt cttaccaaca
     4261 aatagtgatc gggttaggaa ccaatggtgt gataacagat gaagctatag aacaaacaat
     4321 gtctttgctg aaagataaaa aagtatactg ggtaaatata aaagcaccaa ctggctggca
     4381 agatacgata aatgccacat tagctacatt gccaaatcag tattctaata taacgatgat
     4441 cgattggtat agtgagagca aaaatcatcc tgaatttttt tatgatgatg aaacccattt
     4501 gaatgaaagt ggacgtacag cttatgcaaa gtatattgct tcagttattc aataaggtag
     4561 tgattacgtg tgttaacctt actttttcct aataggtgaa tcaatttaaa taaaaaatac
     4621 ttcatatgat aaacggtgtt taagaggagg agctttttaa tattcgtggt aaaaaagaga
     4681 tgggcgaggt cgttaactga gagcttcttg gaaagaaatc gctttggatt tatcctcttt
     4741 gttatacaga acatgagcga gacgacaata ctaggatgta tgttctgatc gaagttttat
     4801 ggttacaagc agcaatcagc ttagataaaa ttaaggatcg taggaaccaa gtaaagaagg
     4861 ctgttagtaa agaaacaaac gatcaagtat gttttactct taagaagatg cagtgaataa
     4921 ataatagtaa tataatgcta gaagggactg tgacaaaaga cgtgtcacag ccttttttgt
     4981 tattttaatt atcgtccagt ctttttttat aaatgattgc ttaagcatta aatattaaaa
     5041 aatatagagt tcaaaattac tgcacaatta gatgaaaaat ttgaacaaca aaaagtgtat
     5101 ttttatacta taatagcttt tgaatttagt tactctattc aaaagttatg ctatggacaa
     5161 gaaaaagaat aaatagaggt gagaaaatga atagtttcaa gaaaaaattt gtgagtagat
     5221 ttttaaatat gaaaaatttt tttgaaaaaa agggagatta tgatgcatat aattatgtat
     5281 taaatctaga aaagcaaaca tattatacac tacgtaaaaa cgatgataat gacttttgga
     5341 ctatgttacc taaaatgctt tcgattgact ctaagctttc ccttttagag gaatttatta
     5401 atttagacat tgaagaattt tgcgggggaa aagagttaat taatatggta gagagtgatt
     5461 atcataccta ttataaagaa agttgtggat acaaagtgga tgagcaatgt ccacaatcaa
     5521 ttatttttta cattgagtaa tatgaactat taagcaggaa agcaactaga agctactgat
     5581 ttagtattta caaatataaa ctatattgct aacaattatt ttctagaagg aataaaagaa
     5641 ctaagaatca agacgttatc ttgattctta gttcttttaa catggaaaag atggataatg
     5701 cactgttaat tcatgatgca tcatcagaaa agttggaagg agaagataga aatcattaaa
     5761 tgatttctac agatggtaaa tgaaaaaaca aaatattagc aaaaatttgc ctaaatagat
     5821 agtaacactt
//
LOCUS       DAYYHB010000123         5779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000123 DAYYHB010000000
VERSION     DAYYHB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.126"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHA6B_002495"
     CDS             <1..813
                     /locus_tag="ACHA6B_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8950034.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHA6B_002496"
     CDS             786..1229
                     /locus_tag="ACHA6B_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950035.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACHA6B_002497"
     CDS             2363..3331
                     /locus_tag="ACHA6B_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8950036.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACHA6B_002498"
     CDS             3341..3829
                     /locus_tag="ACHA6B_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8950037.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACHA6B_002499"
     CDS             4125..4406
                     /locus_tag="ACHA6B_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950038.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACHA6B_002500"
     CDS             4408..4824
                     /locus_tag="ACHA6B_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950039.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5492..>5779
                     /locus_tag="ACHA6B_002501"
     CDS             5492..>5779
                     /locus_tag="ACHA6B_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8950040.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DAYYHB010000124         5728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000124 DAYYHB010000000
VERSION     DAYYHB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.127"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACHA6B_002502"
     CDS             <1..239
                     /locus_tag="ACHA6B_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950041.1"
                     /translation="FLRRYKWYDRTKFQREVREKNEDHRDWTKWVGKIDPRKEDQRNH
                     RLSATAARPFMAYNRLQYSGKKVVFARTAIVYGI"
     gene            130..603
                     /locus_tag="ACHA6B_002503"
     CDS             130..603
                     /locus_tag="ACHA6B_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HFM8950042.1"
                     /translation="MTDFPLLPLDLLWHTTDYSIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRE
                     YLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            677..1117
                     /locus_tag="ACHA6B_002504"
     CDS             677..1117
                     /locus_tag="ACHA6B_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8950043.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            1130..1339
                     /gene="copZ"
                     /locus_tag="ACHA6B_002505"
     CDS             1130..1339
                     /gene="copZ"
                     /locus_tag="ACHA6B_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8950044.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            1339..3525
                     /locus_tag="ACHA6B_002506"
     CDS             1339..3525
                     /locus_tag="ACHA6B_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8950045.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            3545..5716
                     /gene="copB"
                     /locus_tag="ACHA6B_002507"
     CDS             3545..5716
                     /gene="copB"
                     /locus_tag="ACHA6B_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8950046.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         1966 a         1001 c         1291 g         1470 t
ORIGIN      
        1 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
       61 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgaccct cgcaaggaag
      121 atcaaagaaa tcaccgactt tccgctactg ccgctagacc ttttatggca tacaacagac
      181 tacagtattc aggcaaaaaa gtggtttttg caagaacagc aatcgtttat ggtatctaat
      241 ccgtcatgga ttgttgaagg aaattatact tctacattag aggaaaggat taaagaagca
      301 gataagatca tttggcttaa agtgccgcgt tatctcgcca tctaccgagt agtttggcgt
      361 tctctaaaaa gaaagataaa caaaaaatct cggccagata tgcctgaatc attttcagag
      421 aaactaaaca gggaatactt agaattttta tcttttatct ggcattttga agaaaacaat
      481 gaaccgaaaa tacagcagct gatccaagta gctaatgcaa gaaataaact gatcatcctt
      541 aaaacaagac gagagaaaca agaattttta actaaattga aaatagagag caaaaataat
      601 tgacagtttt tgtttacaag cgtaatctgt attcataagc taatgattac atttgtaaac
      661 gaaaggtgtg aaataaatgg aaaaagtcaa aacgccagta aagatcagtg attcagagtg
      721 ggagatcatg cgtgttattt ggacacttgg acagacgaca gcccaagaaa tcacgcagat
      781 cttagctgat tcgatggatt ggaaaccagc aacgatcaaa actttgctgg gacgtttggt
      841 aaaaaaagaa gtgatctgga cagaacaaga aggaaaaaaa ttcatttatc atccagctgt
      901 ctcggagatg gaaacagttc gcagtgcgac ggaaaatctt ttttcccata tctgtgcaaa
      961 aaggattgga gagacgattg cggatctagt agaagaagcc actttgactc aagaagatat
     1021 caatgagatt ttgaaacaac tggaacaaaa gcaaccagta gatacgatcg aatgtaactg
     1081 tatcccaggt caatgtgaat gtaaacaaca tcaataggag gaagaaaaaa tgaaacaaca
     1141 attttcaatc gaaggtatga gctgcaatca ttgtgcagca agagtagaag aaacagtaag
     1201 cgcacttgac ggcgtgcaaa aagtgaaagt aaatttgaaa aaagcaaacg gtacggtcaa
     1261 gtttgatgaa acaaaagttc aatccgaaaa gatttgtcaa gcaatcaatg gattgggtta
     1321 taaagcagag gtgatttgat ggcaacagca gaaaccaaaa tggatacgtt cgtcattaca
     1381 ggtatgactt gtgcaaattg ttctgctagg atcgaaaaag agctgaacgg acaacctggt
     1441 gtggtcaatg ccacagtcaa cttggccact gaaaaagcaa gtgtcaaata tgaagggacg
     1501 accactgaaa aattaatcca aagcgtagaa aacatcggtt atggtgcaat tttgtacgat
     1561 gaagcccaca aacaaaaaat tgcagaagaa aagcaagcgt atttgaaaaa aatgctattc
     1621 gatcttatat tgagtacggt tttgactttg ccgttgatgt tgtcaatgat tgctatgatg
     1681 ttaggaagcc atgcggcgat tgttcacttt tttcattttc caatcgtcca gttagtgctg
     1741 tctgctcctg tccaatttta tgtcggtgct cgtttttata aaggtgcgta ccatgcaatc
     1801 aagacgaaag caccaaatat ggatgtattg gtagcaattg gaacatcggc tgcctttgca
     1861 ttgagcattt acaacggatt tttccgtgga cacccccaag atctttattt tgaaagcagc
     1921 agtatgatca tcacgctgat tttgctcggc aaatatttgg aacatacagc aaaaacgaaa
     1981 acaggtaatg caatcaaaca attgatgtct cttcaaacaa aaaccgccca agtgataaga
     2041 aacgggaaag aagaaacgtt agcgattgaa gaagtagtcg ttggtgatca actagtcatc
     2101 cgtcctggtg agcagattcc agcagatggt cgaattattt caggaagcag tgcgatagat
     2161 gaaagtatgc ttactggtga aaatcttcct gtagaaaaga atccggatga cacgcttttt
     2221 ggcgggacaa tcaatacgaa tggtctgcta cacatggaag tcacacaagt cggcaagcag
     2281 acagtattag cgcaaatcat tcaaatggtg gaagatgctc aagggagcaa agccccaatc
     2341 caaaaaatcg cagatcggat ctcagggatt tttgttccga tcgtattagt catcgcgttt
     2401 atcacgctca tagctacagg tttgatcact ggtgattggc aactcgcatt gatccatagt
     2461 gtctctgtat tagtcattgc gtgtccatgt gcattaggtt tagcgacccc aacagctatt
     2521 atggtaggaa cgggtgttgg tgcacgaaat ggaattttaa ttaaaggcgg agaagcatta
     2581 gaagcagctg ctcacttaga cagcattgtt ttggataaaa ctggaacgat tacagaagga
     2641 aagcccaaag ttacagatct agtcggttca aaagaagtcc tatctatttt ttatacttta
     2701 gaacaagctt ctgaacatcc actaggtaaa gcaatcgtgg aatacggaaa actgcaagaa
     2761 gcagcaacat acgatatgat tgattttaca gcccacccag gtgctgggat cagcggtacg
     2821 attaatggag tacgctattt tgcgggtaca cgtaaacgat taattgaatt gaacctatca
     2881 tttgatgaat accaagaaca cgcgttagaa ttagaacagc agggaaaaac agtcatgttt
     2941 ttagctgatg aaaagcaagt gatcgggtgg attgccgtgg ctgatcaaat caaactagaa
     3001 gccaaacaag caatcaagca gctccagaat aaaggattag atgtttttat gctgacagga
     3061 gacaacaaac tggcagccga aacgattggt aaacaagttg gtattgatcc aaagcatatc
     3121 tttgctgaag tacttccaga ggataaagca gcctacgtag aaaaacttca gaaagatggc
     3181 aaaaaagtcg gtatggcagg cgatggaatc aatgatgcac cggccttagc attagctgat
     3241 gtcggtatgg caatgggaag tggaacggac attgcgatgg aaacagccga tgtgacgctg
     3301 atgaatagca gtttggcatc cattgcacaa acaatcgagt tgtctaggtt gacattgaga
     3361 aaaatcaaac aaaatctttt ctgggctttt gtctataata cgattggtat tccttttgcc
     3421 gcactaggct ttttgaatcc aatcattgca ggaggagcga tggcgtttag ttccgtcagt
     3481 gtattgttga actccctgag cttaaatcga cacatgaaaa agtaagagaa gggatggagt
     3541 aaaaatgaat gggaacgata tggagaaaaa acatgagcat aaaaaagaag tagtaaacaa
     3601 agaaagtaca gaatctcatg aaaaacagat ggagatgaaa catgaccata gtcagatgga
     3661 tcattccatg cacatgggac ataaccatgg agatatagca aacagcaaac aaatggacca
     3721 ttcgatgcac atggatcacg aacacggcgg catggatcat tccatgcaca tggggaattt
     3781 caaacaaaaa ttttggctat cgcttatcct agctatccca atcattcttt tttcaccaat
     3841 gatgggaatg gaatttccat ttcaagtaac gtttcctggt tctgattggc tcgtattgat
     3901 cttagcaact atactattta tctatggcgg tcagccattt ttaagtggag ctaagatgga
     3961 attgaaacaa aaaagtcctg ctatgatgac actgatcgcg atggggatta cagttgcata
     4021 tatttatagt gtctattcgt ttatcgcaaa tctgctcaat ccacatacac acgtcatgga
     4081 tttcttctgg gaattggcta ctttgatcgt tattatgcta ctaggtcact ggattgaaat
     4141 gaatgctgtg tcgaatgcca gcaatgcttt acaaaaacta gcagaattac tgccagaatc
     4201 agtgaaacgc ttgacgaaag atggaaaaga agagacagtt tcattgaaag aagtaaacga
     4261 aggtgatcgt ttgatcgtcc ggtctggtga taaaatgcca acagatggtg taatcttgaa
     4321 aggtgaaaca attgttgacg aatcagctgt gactggcgaa tcaagaggaa tcaaaaaaca
     4381 agccaatgac aaagtgattg gcggttctat caatggggat ggaacgatcg aaatcgaagt
     4441 aactggaact ggtgaaaatg gttatctagc gaaggttatg gaaatggtgc gaaaagcaca
     4501 aggagaaaaa tccaagctag agtcattatc tgacaaagtg gcaaaatggc tgttttatgt
     4561 cgcattaatt gtgggtatcc tggcattcat cgcctggttg ttcttgacag atcttcctaa
     4621 tgcattagaa cgtatggtaa cagtctttat catcgcctgt cctcatgctc taggtttagc
     4681 tatcccatta gttgttgctc gctccacatc aatagcagct aaaaacggat tacttttgaa
     4741 aaaccgcaat gcactggagc aagccaatga tctagatgtg atcatgctgg acaaaacagg
     4801 aacactaaca gaaggaaaat ttactgtaac gggtgtcgaa gtacttgatg atgcgttcaa
     4861 taaaaatgaa atccttcaat atctaggagc cttggaagcg aatgccaatc atccattggc
     4921 tgttggcatt atgaattatt tgaaagaaca tgaaatcaag ccttatcaag ctgaaaatct
     4981 aaaaaatctt tcaggagtcg ggctagaggc aacagtcaaa aaacaacaag tgaaaatagt
     5041 taatgaaaaa gaagttgagc gtctcggatt aaacgtagaa caagcattat tgaaacctta
     5101 ccaagaacaa ggaaatacca tcagcttcct gattttagaa aatcatctag ctgcaatcgt
     5161 tgctttaggt gacgtagtaa aaacagaagc aaaagaattt atccgaacat tgaaagaaag
     5221 aaaaatcact cctgtcatgc ttactggtga caataaaaac gctgcccaag cagtagcaga
     5281 ttatttaggg atcgaagaat attatggcgg tttattacca gatgataaag aagctgttgt
     5341 ccagaaatac cttgaccaag ggaaaaaagt aatcatggtg ggtgatggga tcaatgatgc
     5401 gccaagttta gcccgtgctt caatcgggat ggcaatcggt gcagggacag atatcgcaat
     5461 cgattctgcg gatgtcgtat tgacaaacag cgatccaaaa gatattttgc attttcttga
     5521 tctagccaag caaacgcgaa agaaaatgat ccagaacctc tggtgggggg caggttacaa
     5581 tattcttgca attccgctag cagccggaat tcttgcgcca attggtatca tattaagtcc
     5641 agcagttggt gctgtcttga tgtcgttaag tacggtagtt gtagctctaa atgctttgac
     5701 attgaaaatc aaataataat gaaaaaag
//
LOCUS       DAYYHB010000125         5693 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000125 DAYYHB010000000
VERSION     DAYYHB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.128"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..510
                     /locus_tag="ACHA6B_002508"
     CDS             58..510
                     /locus_tag="ACHA6B_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950047.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="ACHA6B_002509"
     CDS             524..1213
                     /locus_tag="ACHA6B_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950048.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="ACHA6B_002510"
     CDS             1338..3689
                     /locus_tag="ACHA6B_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8950049.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="ACHA6B_002511"
     CDS             3769..4164
                     /locus_tag="ACHA6B_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950050.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="ACHA6B_002512"
     CDS             4489..5106
                     /locus_tag="ACHA6B_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8950051.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="ACHA6B_002513"
     CDS             5147..5446
                     /locus_tag="ACHA6B_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8950052.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="ACHA6B_002514"
     CDS             5480..5647
                     /locus_tag="ACHA6B_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8950053.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT         1996 a          752 c         1161 g         1784 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tct
//
LOCUS       DAYYHB010000126         5689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000126 DAYYHB010000000
VERSION     DAYYHB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.129"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACHA6B_002515"
     CDS             complement(488..1297)
                     /locus_tag="ACHA6B_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8950054.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACHA6B_002516"
     CDS             complement(1297..2043)
                     /locus_tag="ACHA6B_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8950055.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACHA6B_002517"
     CDS             complement(2061..2528)
                     /locus_tag="ACHA6B_002517"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8950056.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACHA6B_002518"
     CDS             complement(2528..2938)
                     /locus_tag="ACHA6B_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8950057.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACHA6B_002519"
     CDS             complement(2949..3962)
                     /locus_tag="ACHA6B_002519"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8950058.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACHA6B_002520"
     CDS             4179..4913
                     /locus_tag="ACHA6B_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8950059.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACHA6B_002521"
     CDS             4897..5607
                     /locus_tag="ACHA6B_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8950060.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAYYHB010000127         5644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000127 DAYYHB010000000
VERSION     DAYYHB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.130"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..78)
                     /locus_tag="ACHA6B_002522"
     CDS             complement(<1..78)
                     /locus_tag="ACHA6B_002522"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFM8950061.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSET"
     gene            complement(484..1173)
                     /locus_tag="ACHA6B_002523"
     CDS             complement(484..1173)
                     /locus_tag="ACHA6B_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8950062.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1218..1982)
                     /locus_tag="ACHA6B_002524"
     CDS             complement(1218..1982)
                     /locus_tag="ACHA6B_002524"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8950063.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2010..2708)
                     /locus_tag="ACHA6B_002525"
     CDS             complement(2010..2708)
                     /locus_tag="ACHA6B_002525"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8950064.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2720..3499)
                     /locus_tag="ACHA6B_002526"
     CDS             complement(2720..3499)
                     /locus_tag="ACHA6B_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8950065.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3515..4459)
                     /locus_tag="ACHA6B_002527"
     CDS             complement(3515..4459)
                     /locus_tag="ACHA6B_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8950066.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4505..5284)
                     /locus_tag="ACHA6B_002528"
     CDS             complement(4505..5284)
                     /locus_tag="ACHA6B_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950067.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1538 a         1161 c          935 g         2010 t
ORIGIN      
        1 tgtttcagaa taattacaat attttgggac cggataactc ccttttgctc gtaaccactc
       61 atatccgtta ggattcacta aaagtttacc gtttattttt cgattctttt tttgaaccgt
      121 cccatgaaca taccgattca acaagctaaa ttataaaaat tggtgtgctg tcattatttt
      181 ttggaaaatt tattaatggc tatcaatata cttttgtatc atactaaata gctcgtcagt
      241 tcctatatcg gatataccaa tattaaagca tttagatcat tatatttaag agtggtatca
      301 tcccaaaatt tcgaatattt tcttttatat aagaataatg atactgaatt gccccattca
      361 cctttctttt tttagatttt ctacaagtga ttcaaaccct ccataattac agttattact
      421 tttgaaacga taatatgaac atgtgtaatc ttttttatta acaatccatc attctatccc
      481 taactaatat gctccattgg gcttgacaat aacataaaac gtttttaaaa tcaacctcaa
      541 gtcgtacatt aaacttcgat gactaatata ctctaaatcc aactcaacca ttttatggaa
      601 actaacatta tttctatcac taacttgcca caaacctgaa caaccaggtt ttacatacaa
      661 gcgttgaaga tcatatctcg tgtactcagc tacttccctc tccaaaggtg gccgtggacc
      721 aattagactc atatcgcctt ttaaaacatt ccatagctgt ggcaattcat caatgctttt
      781 ggcacggata attttaccta ttttagtgac acggggatct tctttcatct tgaacattgc
      841 accttctatt tcattttgat caagtaattc ttccaatttt tcttcagcat ctaaacacat
      901 cgaacggaat ttatacattt tgaatgtttt tccattttta ccaacacgtg tatggcaaaa
      961 aaatatcgga cttcctggct cttcttttct gattcgataa gctacataga acatcaaagg
     1021 agaagctaaa atcaaaccaa taatgcttcc tacaatatca atcattcttt taaaaaataa
     1081 atagatatat cgtttttcaa ctttttcttt atccaaccaa acttgattag gatgtacttc
     1141 actaatacga gataataaat cttcactttc catactttcc ctctctcctc atttcctttt
     1201 tatctacgaa taaagaacta aaataaacca aacttcttct tttttacctc ttcatagact
     1261 ggataactga tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact
     1321 ttttccttac caaagtcctt agcaatctgc tcatagcact cttttaagta aaaggtacgc
     1381 ttagagaccc catgggcatc agaagcagcc atctgcacga gattatgttt gaccattgtt
     1441 ttggctgttt tctgaatgtc tttgccaaat acaccaacat aacttggcgc agtaatttgg
     1501 gccaaacatc ccatatctag gaacggaagc aaatgatttg ggtcttctcg aaaatgcgcg
     1561 ttgcgttctg gatggacgat cactggtgtc tttcctagtt ctaacaactc aaaaaaaagc
     1621 cattctgcat ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct
     1681 aaatctgtaa ataaaatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt
     1741 acttcttgtc cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac
     1801 gaagcgacta gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt
     1861 ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt tttcagccat tgcaatactt
     1921 gcttctaagt tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc
     1981 atttctcttg tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg
     2041 ctatgttctg ctccgttgta tacaacacct aaaactcgtg cttgtaccat ctctagcagt
     2101 tcttttgctt tattcaatga ttctttacgt gatgtatttt cacgtacgac caataaagtg
     2161 ccatccgttt ttgaagacat gatctgtgca tctgttacgg ctactactgg tggcatatcg
     2221 aatatcacca cgtcaaataa ctgtcttgct tcgtctagga tctgatccat tcttggcgaa
     2281 cttaacaatt ctgatgggtt gggtggctta ggtccactag gcaaaatcga caagttctcg
     2341 actggcgttc gttggattac atctgctaca cttcctgatg aactaagtgc tgtacttaat
     2401 cccctggcat tgttcagctg gaatgtctta tagacgacgg gtttacgtaa atctgcatcg
     2461 actagtagaa ctcgttgacc ggatttagca aaaacgactg caatgtttgc tgcagtggtg
     2521 gattttcctt ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct
     2581 gcggaggcaa actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat
     2641 gggtttgtta atgtgatcaa actgactgca tgagtttgtg ctgctttttg tttctgtgtt
     2701 cgtgccattt ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat
     2761 cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt acggatacca gcgttcaatt
     2821 ctttcgctga catttctgga actgttccta agattgggaa tcctaatgca tccgtcacat
     2881 atttttcatc tttcactgtc cgatcaagta attcgaataa gaatgccaga cctacgccta
     2941 ccatcattcc taatactaag ccaatcacta gattcaattt attatttggc gaaacaggtg
     3001 tcgtattggc aactgcacct gaaataatcg agattttatc gacattcatc acgtcttttg
     3061 cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg ttgagcagtc actgcattcg
     3121 tgcttattgc ctggatcgag aacatttgtg aattttgtga ctgattgacg gagatcgcat
     3181 ctttgatttg tccagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt
     3241 catttagtac taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg
     3301 tattgacatc attgacattt gctgtgtctg attgtggcag tgtcacgatc aattgtgctt
     3361 gtgaactgta ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag
     3421 caaacattga gatgatgatt gttaccaagt gtttgcgtaa tgtttcaaaa atttcttcca
     3481 agttgattgt ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat
     3541 cttttaattc tttttttaca cgatcaagtt cttgatcact gactttttga taggagatat
     3601 tatcttgcat aaagccttct ccttgcagct gatcttgctt gatcgtacca aatgcctccc
     3661 gataatctaa agcgattttt ttcatttcat caaagctcat gtctgttttc atattcgact
     3721 ctagagcatt caaaacggat tgatagttgc tgacaccgtt taaactcaat actttttttg
     3781 ctacaccttc tacgatcttt ctttgacgct cttgtctacc atagtcacca tttggatctt
     3841 cataacgcat acgagaataa gataatgcct gatccccgtt caatgtgatt ttaccgattg
     3901 taaagtcata gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg
     3961 catcaacaag ttcctttagc cctttcatat tgatagagac atagtgatcg attgggatgt
     4021 ctacatattt ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt
     4081 tgatcttgtc ttttgtgtca cgacctacga tgtcgacata cgtatcacga ggaatgctga
     4141 cgatcgttgt ctgtttgtct tcaggactga ctatggcaac catcatcgta tctgatcttc
     4201 cttgatcgac ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt
     4261 cttgtttgtc cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt
     4321 aagttttctc aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg
     4381 ctaatactaa tacgataaga actgctaaaa taatcagaaa tatttttttc cctcttgatt
     4441 ttcttcttcg tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta
     4501 actgtcaata acttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg
     4561 cgtatgctgc gcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat
     4621 attgatcgta agtagcggtg gcagtcactt gaccatcctt caacatcgta tcgaataact
     4681 gatctagttg cgtgacttgc gcgatctgag tatcagcgaa tcctaaatat tgcataacaa
     4741 ccgttttcca aggactatct ttaaaaaagc tcatattttc acgtacacga gcgacatctg
     4801 cacttgtcac cttttctttg atgatgacat cgtctacagc ttgctgccag tttgatacat
     4861 ttttagtaaa caaatcgttg acagctgtct ggatcttcag ttttgattct gcactatcta
     4921 actgtgtcac gattgctttc ttctcatctt ggattgcaac agcttcttta ggcaattctg
     4981 attctttgat gtcgtaatca tctgctgaaa gtttgacata ggatagctta tttgacaaac
     5041 ttgtgattgt ttcttctgtt atttcagggt ttagaaaaat tttttctttt gtactataca
     5101 gcttgtctac ttcttttgat aaagaagtca aggtgtcttt ttgattggtg atggcagatt
     5161 ctgcataatt gatccgttgc tgatgttgcc gctgattatt gatatggtaa ccaaacaaac
     5221 agatcgcaat agcacctgca cctaccgcca cagcagtcaa tgacttgacg aatggctttt
     5281 ccatagattc actcctaagt attgatgata gataaattaa gatataattc acttacataa
     5341 aaatgacagc tttttatttt aaaaaacata attaaactaa tatattcaca gaaatatttg
     5401 taaacaagtt ttccctatat aatcatacca caaactctct tcttgactag ggcaatttat
     5461 gatttaaaag taaataaaac attaataaga taacaattaa tcgatttcat ttttatttcc
     5521 ttaattttca taggtgtttt taaaagttac acaaaaaata aaataacaaa aaaattaaca
     5581 ttgaattaat aatagttctt aattgtcaaa ttaagttaga cagatcatca atacttacgt
     5641 aact
//
LOCUS       DAYYHB010000128         5595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000128 DAYYHB010000000
VERSION     DAYYHB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.131"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..863
                     /locus_tag="ACHA6B_002529"
     CDS             6..863
                     /locus_tag="ACHA6B_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8950068.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ACHA6B_002530"
     CDS             885..1394
                     /locus_tag="ACHA6B_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8950069.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ACHA6B_002531"
     CDS             1431..1832
                     /locus_tag="ACHA6B_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950070.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ACHA6B_002532"
     CDS             1944..2297
                     /locus_tag="ACHA6B_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950071.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ACHA6B_002533"
     CDS             2331..2774
                     /locus_tag="ACHA6B_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8950072.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ACHA6B_002534"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ACHA6B_002534"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8950073.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ACHA6B_002535"
     CDS             3869..4582
                     /locus_tag="ACHA6B_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8950074.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ACHA6B_002536"
     CDS             complement(4789..4938)
                     /locus_tag="ACHA6B_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950075.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="ACHA6B_002537"
     CDS             5334..5534
                     /locus_tag="ACHA6B_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8950076.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1941 a          856 c         1216 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaatt
//
LOCUS       DAYYHB010000129         5556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000129 DAYYHB010000000
VERSION     DAYYHB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.132"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1251)
                     /locus_tag="ACHA6B_002538"
     CDS             complement(<1..1251)
                     /locus_tag="ACHA6B_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8950077.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
     gene            complement(1456..3264)
                     /gene="pepF"
                     /locus_tag="ACHA6B_002539"
     CDS             complement(1456..3264)
                     /gene="pepF"
                     /locus_tag="ACHA6B_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8950078.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(3440..4438)
                     /locus_tag="ACHA6B_002540"
     CDS             complement(3440..4438)
                     /locus_tag="ACHA6B_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8950079.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(4664..5317)
                     /locus_tag="ACHA6B_002541"
     CDS             complement(4664..5317)
                     /locus_tag="ACHA6B_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8950080.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1630 a         1151 c          923 g         1852 t
ORIGIN      
        1 tttgaaattg taaaagttgc gaaagccgta cgcattgcgt tttaatactt tgatgtggtt
       61 gtttaagcat tccaatggtc cgtttgaata gctgtagtga agcgcatttt ggatctgtgg
      121 cagaaacttt ttaaacgttc gtaaagtggt tgtataggtt tctggcagac cgctaatgtc
      181 tctagttaaa caatcctcaa atagtgactg atctcttttc tttaccgcat aaagaatgtc
      241 ttggtataac tgatagcctt ggcgtaagtt gggacaagca gctaacagtc gatcggcaat
      301 ctcactttca gtcaaataat ctttaaaact ggcacgccaa acccgttttt catagtttaa
      361 ctttgcttct tctttttgta ggagtttcca atagcgcttc aattttttcc ctaaccctcg
      421 ttgagagtgg gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa
      481 agttctgcca atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa
      541 aagctttttc accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc
      601 ttcacgagtc gctaatgaaa accgagaaaa atagcgttct aaataaggca agcgtcgatt
      661 ttcgacaata tccaataatg catggctttg tccgtccatc atgataaaac tcattgcact
      721 ggcaacactt cgaaccgatt tgaattcatc aaagcataag acctttggca aagtctgttt
      781 gaaaggattc aatgggcggt agaatcgctt catgacacga tagaccgaag aagtggaaat
      841 atttttctga cgagcgattt ccgtcatgga agtattctct tttaagcgag caatgacaga
      901 ccaacgaact ttttgactaa tgaaacagtg tttttcagca acagaggtat ccgctacgaa
      961 tgttctttgg caagacttac atttaaatcg ttgtttcttc agctccagat aagtgcgata
     1021 ctcagacaca tcattcagta acaaccgaac ggttgtcgtc ccccaacgaa tgatcctaga
     1081 ggggacgaca caaagacaat gggaacactc ttctggcttg tacagaagag ttcctttgta
     1141 aatcaagcaa atttgccctt tgtatttttc tttagttaaa caattttcct cgaaatagat
     1201 atttaggtct agaatattta gggtatcttt gataagctga gtatgagaca tcttgaatcc
     1261 tcctatgaat tggtagccaa attaattcta aaggatttca gggtgtcttt tctatatttt
     1321 tttatacaaa aaagatgttg gtgaagttca ccaacatcaa aaagtataga gccttaaaaa
     1381 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
     1441 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
     1501 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
     1561 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
     1621 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
     1681 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
     1741 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
     1801 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
     1861 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
     1921 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
     1981 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
     2041 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
     2101 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
     2161 gcttcccgaa gaataagcac cgcttcgttt ccctttattt tcgacaacat cgatccaacg
     2221 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
     2281 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
     2341 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
     2401 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
     2461 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
     2521 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
     2581 ttgttcgtaa acactgtaca accccttaaa tgctgcttcg cgcacccttc gatccgtgct
     2641 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
     2701 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
     2761 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
     2821 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
     2881 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
     2941 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
     3001 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
     3061 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
     3121 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
     3181 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
     3241 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
     3301 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
     3361 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
     3421 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
     3481 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
     3541 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
     3601 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
     3661 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
     3721 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
     3781 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
     3841 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
     3901 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
     3961 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
     4021 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
     4081 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
     4141 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
     4201 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
     4261 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
     4321 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
     4381 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
     4441 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
     4501 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
     4561 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
     4621 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
     4681 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
     4741 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
     4801 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
     4861 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
     4921 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
     4981 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
     5041 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
     5101 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
     5161 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
     5221 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
     5281 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
     5341 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
     5401 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
     5461 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
     5521 tatttttcgt gcaatcatca tcttctacta cctttt
//
LOCUS       DAYYHB010000130         5456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000130 DAYYHB010000000
VERSION     DAYYHB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.133"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..556
                     /locus_tag="ACHA6B_002542"
     CDS             <1..556
                     /locus_tag="ACHA6B_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HFM8950081.1"
                     /translation="NLIRGEFHFLSEKDMNIIPSFKDSGYIPDTKAMLIYDETGQLEN
                     YLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARSLELLSQLQKNTLQL
                     IRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFEAYKNSLLLVMDLQS
                     HLIEQYNLKVTHDILERLLNYISE"
     gene            1244..2065
                     /locus_tag="ACHA6B_002543"
                     /pseudo
     CDS             1244..2065
                     /locus_tag="ACHA6B_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2208..2432
                     /locus_tag="ACHA6B_002544"
     CDS             2208..2432
                     /locus_tag="ACHA6B_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950082.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2447..3316
                     /locus_tag="ACHA6B_002545"
     CDS             2447..3316
                     /locus_tag="ACHA6B_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8950083.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3297..4031
                     /locus_tag="ACHA6B_002546"
     CDS             3297..4031
                     /locus_tag="ACHA6B_002546"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8950084.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4064..4972
                     /locus_tag="ACHA6B_002547"
     CDS             4064..4972
                     /locus_tag="ACHA6B_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8950085.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4969..5196
                     /locus_tag="ACHA6B_002548"
                     /pseudo
     CDS             4969..5196
                     /locus_tag="ACHA6B_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5220..>5456)
                     /locus_tag="ACHA6B_002549"
     CDS             complement(5220..>5456)
                     /locus_tag="ACHA6B_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950086.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1865 a          821 c         1159 g         1611 t
ORIGIN      
        1 taatcttata cgtggggaat ttcatttcct ttctgaaaaa gatatgaaca taatcccctc
       61 gtttaaagat tcaggttata ttcctgatac gaaggctatg cttatttacg atgaaacagg
      121 gcaattagaa aattatttat cagagataag tggtgcaaga ccaaatagac ttactgaaga
      181 aaatgctaat tttttgttgt gtaatttctc taatctatgg ttgatgggaa tcaacgttct
      241 aaaaagagga gaatatgctc gttcattaga actcttatca caacttcaaa aaaatacact
      301 acaacttata cgtatggcag aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct
      361 tgaaaaagaa attagccttg aaaattataa aaaatttgca aagaccactg ctcgattaga
      421 taaggtagaa ttatttgaag cctataaaaa ttctttgcta ttagttatgg atttgcaaag
      481 tcaccttatt gaacaataca acttaaaagt tacacatgac attttagaaa gattgttgaa
      541 ttacattagt gaataggaga gtaacgttat tcacagtatt gggtactgaa aattaacagc
      601 atacctcagt ttttcaaaaa acagcgaagc ctatggatat gttcccgtct accgatagtg
      661 caaaaagtgt cgtggatatg tttccgagaa cggacataca caaatgacat tcattctgtc
      721 ctctcaatcc atgtggaagc agtatgtctg ttaacaagaa aataattatt gttcgtcctg
      781 acgagttgtt agactcgaaa aggataaaca tcatttttag aaccggtagc cgatttttgg
      841 ctagcggttt tttgggctct ttggcaaata gtggtgattg aatctcgcgc tatcctatgg
      901 aaaaccgcct tgtgtgaggg gctgcgagac cgcttcgatc ctagaattgc tgaagcacga
      961 atctagcgtc actcacattt catcttttga cgtagctgag acaaggtgtt tttcttcgat
     1021 ccgctagtgg ttaatctctt tttggtgcaa atatggttta gaatttcttt gcatggctta
     1081 agggagtgga gaatcggata ttagtaggat ttacatccac agataaaaat aggcttgatt
     1141 ttaacagcga aactaaaata ccctacttaa atgaagtgat agattagatg ttgggttgtt
     1201 aggagatgct taaaaatcct atgcaatcaa tagtttttag tgtttgttga cttgatccct
     1261 aaatcgcctg agtactaaac gttgagtgtg tctgcttact gtcactagcc agtaagtgag
     1321 actgtagtta atctctcatt ttccccagta ttatgttgaa aatagccatg aagcgattat
     1381 ttctaaagag ttatttttgc aggctcaaga agaaatttat cggagaagta atatctacac
     1441 aggagaaggc aagaacaaaa gaatttatag tagcaagtac gctttaagtg ccatcacctt
     1501 ctgtggatat tgtggcgata tttataggag aacctattgg aatattcatg gcagaaaaga
     1561 atttgtctgg cgatgcgtga ctagaatcga gcagggtccc gaggtatgta agaaccgaac
     1621 cgtaaaagaa gatgaactct atggtgcggt aatgaccgca attaataagc tacttgctgg
     1681 tggcaacaat atgataaaga cactggaaga aaatattcat gctgtgattg gagaaaccac
     1741 agaataccaa atttcagaga ttaataccct actagatgaa aaacaaaaag aactaatcaa
     1801 gctggccaat aaaggtcaag attatgaata cctggtagat gagatcgatg agatgcgaga
     1861 taagcgtcag actcttttag tagaagatgc ctccctcagt ggcgagaacg agcgcatcaa
     1921 tgagttgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     1981 tgtaaggaag ataatccaga acgttaaggt ctatgaagac cacttcgtca tagccttcaa
     2041 accaggtatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctgaagtag
     2101 agttgtgggt tttcttttaa ttgctgatta taataatatt ttaatgacta ttgcatttat
     2161 caaccaaatg gtttatgtta ttcttagcat aaacttggag gtgctgtatg actacggcag
     2221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     2281 ttggccagac tccacagaat tttaataaaa aattacaacg agaaacagta accttggatg
     2341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     2401 atggagatga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     2461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca ttgtattagg
     2521 gggctcacgt gcaagaagca cccatacaga aaattctgat atagatatcg gaatctatta
     2581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     2641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     2701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     2761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     2821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     2881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     2941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     3001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     3061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     3121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     3181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     3241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     3301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     3361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     3421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     3481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     3541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     3601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     3661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     3721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     3781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     3841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     3901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     3961 atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     4021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     4081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     4141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     4201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     4261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     4321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     4381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     4441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     4501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     4561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     4621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     4681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     4741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     4801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     4861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     4921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     4981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     5041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     5101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     5161 gaatatattg acaatactga taagataata tatcttttaa cccgtggtgc tagttaattt
     5221 taatatccta aagaatatct caaagttcct ccaaaacgtc gttgtgctcg acttaataga
     5281 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     5341 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     5401 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtgg
//
LOCUS       DAYYHB010000131         5311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000131 DAYYHB010000000
VERSION     DAYYHB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.134"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..1087
                     /locus_tag="ACHA6B_002550"
     CDS             212..1087
                     /locus_tag="ACHA6B_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8950087.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1191..1955
                     /locus_tag="ACHA6B_002551"
     CDS             1191..1955
                     /locus_tag="ACHA6B_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8950088.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1942..3384
                     /locus_tag="ACHA6B_002552"
     CDS             1942..3384
                     /locus_tag="ACHA6B_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8950089.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3395..4725
                     /locus_tag="ACHA6B_002553"
                     /pseudo
     CDS             3395..4725
                     /locus_tag="ACHA6B_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4738..5196
                     /locus_tag="ACHA6B_002554"
     CDS             4738..5196
                     /locus_tag="ACHA6B_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8950090.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1803 a          771 c         1111 g         1626 t
ORIGIN      
        1 ttttttgttt ttatgtaatt acctccaaat caattcgata caagtattat cataaaacga
       61 gtaaaaaaga tattgataac gcttttatta tgtgttaaac tatttttgta aattatgtag
      121 ggtgctacta ttcaattagg cacgacctaa gaaagggttc tttcttaggt cgttttttgt
      181 ctttaaaaat tgtttggaga caaaaatatg tatgaaggag tctgcaagtt gttttgtttt
      241 atcatatagt ggaggtgaat taacggtgta tgtgatgaaa aagctgaatc ataatgctgt
      301 attgatcgat gacgatggaa tagaaaaaat tgcgatgggg aaagggattg gcttttctgc
      361 taaagtgagt aagccatttg atgttgagga tgctgacaaa ttatttgtat tagattctaa
      421 agaaaagatc aaacgattca gtgagatagt ctcgcagatt ccaatcgaat ttatggattt
      481 tgcggaagaa gtgattgaac tgataacagc taggatctcc tctccattag attcaaatat
      541 ctatattaca ttgacagatc atatttattt tgctattcaa cgaaaaagag aagatagaga
      601 tgtcacagca attatgttgc cagaaatgag attcctttat ccaaatgaat atgatatagc
      661 aatagaagtg gtcgagcgaa tcaatcatcg atatcaaacc gagttagggt caaatgaagt
      721 aggattcatc actatgcata tcattaatgc tgagctagga gagcctaata gtttaaagag
      781 cttaaaaata gtagaaatga cgaccacgat tttgtcccat ctagaaaaat cctattcaaa
      841 cgtttatgat aaagaatcac tgacttatca tcgcttaatg gttcatatca aattcctggt
      901 taaacgcttg atttatcagg aagaaacgcc agtagaaaat attggttttt ttactgaaac
      961 atttaaacat agcaaagcgt attgtacagc actaaagatt aaagatatca tcgaacagca
     1021 atttcaactt gccattcaag aaaatgaaat catctatttg gcaattcatc tagcacgaat
     1081 ctattaaaat aagggcgtaa ctatctatag gcgcaaccaa atgacacata gtaaatggac
     1141 tatgattatt tggttgcgcc tattttagta gaaaaaagaa ggagaatgaa atgggaaaag
     1201 tgatttttaa ttcggatgat tttggctata gccatggtgt gaattatggg attatggatg
     1261 cttatcagcg aggaattttg acatcaacta cattgatggc aaatatgccg ggtttcgagc
     1321 acgctgtgaa actaagaaag gaaatgccta ggctaggtgt aggagtccat ttaacattga
     1381 cgtgtggcaa accacttctc aaaactgtcg atactcttat cgaaagcgga gagttcagat
     1441 ccttatctta ttatcagcat ccatttaaca ttgacaaaga tcaactgtat caggaatgga
     1501 atgctcagat tcaaaaaatt tatcatgcgg ggatcatacc aactcattta gacagtcatc
     1561 atcatactca tactttggat gaaaaccagg aggtagttgt tacattagca aaaaaatatg
     1621 atttgccggt tcggagcaac tttgaacgaa aagatgaagt tcgtcatgtc gattattttg
     1681 agccttgttt cgatgtcgta ggagaaactg atgaaaaaaa acgacaaatc aatacgagtg
     1741 tggaagaata tttggcaagt ttactcatga tattgagaga agataaaacg atcgaagtca
     1801 tgtgtcacgc agcgtatttg gatcaggaat tgttaaatgg ttcaagtttt gtgtatcctc
     1861 gtactcacca agtcgaattt ttaattcatt cagcatttgc tcaaaaagtg aaaaatgatc
     1921 ctttgattga gttagtaaca tatgaggata tttaatagag gaggaataaa aatgaatgtt
     1981 aaatcaaatg tgcagaagct tggaagagcg atgctacttc cagttgcagc gatgccatta
     2041 gcgggattga tcatgcgttt atcagctgat gatatgctga acatcccagt aattggtgca
     2101 gctggaaatg ccgtttttgg aaacttggat atcttatttg ctataggagt tgcaatcggt
     2161 tttgtcaaaa ataaggacaa aggaatacct gcacttactg gttttttagc gattgctacc
     2221 ttgaaaaaag gcttagaaat catggaccca gaagtgaata tggggatttt tggtggaatt
     2281 atttccggtt tagtagcggc atggacgtat aatcgcttta aagaacaaaa attaccagta
     2341 gttttttctt attttgcggg cgaaaaattc ccattgacta tggtgatgat tctacaaaca
     2401 atcgcagcta tttttttggg ctttatctgg ccgctcatcc aaattgggat tgataatttt
     2461 gcaaaactgc tcgttgatat gggggcatta ggtgtgggga tatttatgtt tttgaatcgt
     2521 ttgctgatcc cctttggact gcaccatgtg ttgaatacgt atatttatta cgatttagga
     2581 agctatacat ctcctagcaa ggaagttttc agaggagaga tgacacgctt tatcaacgga
     2641 gatcctcagg ctggactgtt tttatcaggt ttttttgtag taatgatgtt tggtatacca
     2701 gctatatgtc tagctatcta taaagcagct tataaagaaa ataaagagat ggtcaaagga
     2761 atcatgggaa gcggtgcagg aacttcattt attgcaaata tcactgagcc tgttgaattt
     2821 agtttcatgt ttctttctcc tatgttgtac gtgatccatg cactttttgc cggtttagca
     2881 gggattgtct gttacttatt caatatacga attggtttta cattcggagc atgtattgtt
     2941 gattatctca tcaattttcg aatcgctacc aatgctattt tgattcttcc aatcggaatt
     3001 ttcttttttg ccttgtatta cgttactttt tattatttga tcaataagag aaacattcag
     3061 acattaggac gtgaagctaa ggcagagttt ggcaatgaag taaccttaga agaaacagag
     3121 ttagggttag caagtaaaaa ttatgaatat atggcaacaa aaatgctaca agcgtttgga
     3181 ggaaaagcca atatcctgga tgtctatagt tgtaatacac gcctacgggt agaggtagtg
     3241 gatcccacca tggtcgagga acagcgaatc aagcaattag ggataagcgg gatcattaaa
     3301 ccaactgaaa aaaattatca aattatcatc ggcttagaag tgacttatgt tatggcagaa
     3361 ttcaataaat tattagaaga atagaaagga aaagatgatg atgaaaaaac agattatcac
     3421 tatagctgga ggcgggagta cgtatacacc aggaatcgtt caagccattt tgaacaatga
     3481 aaaacggttg ccattatctg aaatacgttt atatgatatt gatgaagaaa gaaatatgga
     3541 tatgtacttg attgttaaat ttatgctgaa aaaaaaggtt tttcaaatat tcgcattcgt
     3601 gcaacggatg atccaaagct tgcttttaca ggatgcgatt ttgttttctc acaaattcgt
     3661 gttggaggat tggagatgcg agaaaaggac gaaaagattc cacttaaaca tggattagta
     3721 ggacaggaaa cgtgtggact cggaggattt gcttatggga tgcgctcaat caaaggttta
     3781 ttggaaattg tcgatcatat acaagactat gcccccaaag catggatttt gaattataca
     3841 aatccagagt cgatcgtttc tgaagctgtt cgtcgtaaat atccgaatgt aaaaatgatt
     3901 aatgcctgtg atatgactat ttcaattgaa gaaacgattg cagtgaatta cggttatgat
     3961 cgtaagaatt ggatcgttac gtattatggt ttaaaccact ttggctggta tacatcgatt
     4021 tatgataaag aattgcaacg agaaattatg ccagagatta ttgaaaaact aataacaaaa
     4081 gaaatgcaag tcgccgattt taatatagga gacaaaactt ggcaaaaaac ctttcaaatg
     4141 atgtcagtca tcacgaaaaa ttttccaagt aatatcccga ataattactt agagtattac
     4201 ttatatcctg atatggtcgt tgagcatact gacaaagagt atacacgtgc taatatggtc
     4261 atggatggac gagagaaaaa taccaaagaa atggctgata aaatacgccg cggtattgtg
     4321 gaagaagtat tgaactttaa ttttggggag catggacaat atatcgtcga tatagctatt
     4381 tccttgttga atgatgatcg tcgtcgcttt atgctaatcg ttcctaatca aggggcgatt
     4441 ccgaatctac ggtcagatgc agttgttgaa ataccggcat atgttggtgc aactggtgtg
     4501 gagacaatta cactaagaaa accaatcagt gattttcata aaggattgat ggaggcacaa
     4561 gttgcagcag agaagttact agtagatgct tactttgaag gttcttatca aaaagcttta
     4621 caagcattta cattgaatca aaccgtacca aatgccagag tcgcaaaaaa aatactagat
     4681 gaaatgatca aagtaaacaa aaaatattgg ccagcattaa agtagaggtg ataaaaagtg
     4741 catttcttta aaacaagaaa cattctaaaa gctgtagcta gtggtcaaat tattccatta
     4801 acagcagtaa aagacgaggt cttttcttca aaaatgatag gagttggata cgctataacg
     4861 aatcatgatg gatatatcca tgcgccagtt aaaggaataa tcgataatat atttcctacc
     4921 cgacatgcca taacgatcga aagtgaatct ggcagaaaaa tactgatcca tatgggttta
     4981 gatacagttg aactcgaagg aagtccgttt cagatacggg ttgaaagtaa ccaggaggta
     5041 aaagaaggga cactgttagc tattatagat ttgaagaagt tggagcaagc acaaaaagat
     5101 acggtgatca ttgtagtttc tccaggcatt tttgaaggaa atttattaaa acaaaaccaa
     5161 gaagtaaaga ttaaagatag ttgttttgaa ttttagtgta cgatgaaaaa agttaaatag
     5221 ggtgatattt atgggtgaat ttacaaaatt tttttgaaat gataagtttc aatcactaga
     5281 tttaatcatt ttgatttttg ttaattatcg a
//
LOCUS       DAYYHB010000132         5308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000132 DAYYHB010000000
VERSION     DAYYHB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.135"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1197)
                     /locus_tag="ACHA6B_002555"
     CDS             complement(58..1197)
                     /locus_tag="ACHA6B_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8950091.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1506..2219)
                     /locus_tag="ACHA6B_002556"
     CDS             complement(1506..2219)
                     /locus_tag="ACHA6B_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8950092.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2473..3174)
                     /gene="nagB"
                     /locus_tag="ACHA6B_002557"
     CDS             complement(2473..3174)
                     /gene="nagB"
                     /locus_tag="ACHA6B_002557"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8950093.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3408..4940
                     /locus_tag="ACHA6B_002558"
     CDS             3408..4940
                     /locus_tag="ACHA6B_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8950094.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(5131..>5308)
                     /locus_tag="ACHA6B_002559"
     CDS             complement(5131..>5308)
                     /locus_tag="ACHA6B_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8950095.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1746 a         1080 c          892 g         1590 t
ORIGIN      
        1 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
       61 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
      121 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
      181 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
      241 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
      301 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
      361 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
      421 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
      481 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
      541 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
      601 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
      661 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
      721 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
      781 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
      841 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
      901 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
      961 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
     1021 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
     1081 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
     1141 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
     1201 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
     1261 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
     1321 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
     1381 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
     1441 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
     1501 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
     1561 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
     1621 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
     1681 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
     1741 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
     1801 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
     1861 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
     1921 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
     1981 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
     2041 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
     2101 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
     2161 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
     2221 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
     2281 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
     2341 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
     2401 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
     2461 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
     2521 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
     2581 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
     2641 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
     2701 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
     2761 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
     2821 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
     2881 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
     2941 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
     3001 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
     3061 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
     3121 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
     3181 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
     3241 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
     3301 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
     3361 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
     3421 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
     3481 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
     3541 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
     3601 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
     3661 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
     3721 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
     3781 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
     3841 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
     3901 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
     3961 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
     4021 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
     4081 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
     4141 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
     4201 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
     4261 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
     4321 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
     4381 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
     4441 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
     4501 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
     4561 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
     4621 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
     4681 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
     4741 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
     4801 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
     4861 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
     4921 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
     4981 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
     5041 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
     5101 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
     5161 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
     5221 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
     5281 tccaataaag tacataaaca taaataca
//
LOCUS       DAYYHB010000133         5291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000133 DAYYHB010000000
VERSION     DAYYHB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.136"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1171
                     /locus_tag="ACHA6B_002560"
     CDS             86..1171
                     /locus_tag="ACHA6B_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950096.1"
                     /translation="MRVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPTATNVIDLQSKEAIREE
                     TTVNTILNAVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLTSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIATWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLVYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFIQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1168..3066
                     /locus_tag="ACHA6B_002561"
     CDS             1168..3066
                     /locus_tag="ACHA6B_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950097.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKQGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYEDFYFDGEEWDKDIWDRRKLPLPDDKVNPTQYEYVINFKGVRNTYF
                     KQLVKRYCKLRLNTDSFSYVCDIAQKLKEFFNFLDMNFKHVQRINQLTRMEIEAYLSE
                     LNMMGIKPSTINGRISILEGLFSTLLRLEWNDIPSKVLIYPEDYPKIPRAKPRFIDEY
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDYFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSWEFPDSEYLFPRKDGSPLKQETFRDELNKLAYEQ
                     KIVDKSGEIYRFHAHAFRHTVGTRMINNGVPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KAEFTKFKEKLVTNNGDIIELDNDNEVDDVDLQWFKKNINAQVLPNGYCRLPVIAGSC
                     PHANACLDCTHFCTSKQFLSQHEEQLNRTEELLAVAKEKQWQRQIETNNRVKERLEQI
                     IGSLKGIS"
     gene            3067..3444
                     /locus_tag="ACHA6B_002562"
     CDS             3067..3444
                     /locus_tag="ACHA6B_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8950098.1"
                     /translation="MVKQVRNTEEIVRLAKQKSRRTRENVDKVISKLSLEGKTINFNT
                     VAKEANVSKSWLYKEHDIRQRIESLRKQQKTENVISKPKKSSRSEEVLIKTLKTRVKE
                     LEEENIRLRNQIQKLYGDLYIRE"
     gene            3762..4565
                     /gene="lnu(G)"
                     /locus_tag="ACHA6B_002563"
     CDS             3762..4565
                     /gene="lnu(G)"
                     /locus_tag="ACHA6B_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(G)"
                     /protein_id="HFM8950099.1"
                     /translation="MLKQKELMARVKELVQSDERISACMMYGSFTKGEGDQYSDIEYY
                     VFLKDDTISTFDSAKWLNEVASYTLLYQNEYGTEVVIFENLIRGEFHFLSENEMNIIP
                     SFKESGYIPDTKAMFIYDETGQLELYLSELEGPGPNRLTEENVNFLLNNFSNLWLMGI
                     NVLKRGENARSLELLSQLQKNILQLIRIAEENADNWFNMTKNLEKEISPENYEKFKKT
                     TARLNELELYEAYKNSLLLVMELRNLVEKQYQLTISDDFLGKLFNYMNE"
     gene            4689..4838
                     /locus_tag="ACHA6B_002564"
                     /pseudo
     CDS             4689..4838
                     /locus_tag="ACHA6B_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4988..5188
                     /locus_tag="ACHA6B_002565"
     CDS             4988..5188
                     /locus_tag="ACHA6B_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8950100.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         1988 a          715 c         1036 g         1552 t
ORIGIN      
        1 atgttaaagg gagattttac tttaaataca aattaacaag gtttataatt tgtagtactt
       61 aaagtgttta aagagggggg agcgtgtgag ggttcaaagg atagaagtgg agaataagcc
      121 gtatccattg tatttattac tagataaaga ataccagcta attgaaccag taatgaaatt
      181 tattaaatac ttagataaca ctggtaagtc tccaaatacc attaaagcat actgctatca
      241 tttaaagttg ctgtatgagt tcatggaaca gagaggcgtt attcttaatg atattaactt
      301 tgagttgtta gcagacttcg taggttggtt gagatatcca acagcaacaa atgtaattga
      361 tcttcagtca aaagaagcca taagagaaga aacgacagtg aatacaattt taaatgccgt
      421 tatgagtttt cttgattatt taagtagatt aggagaattt aaatcaattg atgtatttaa
      481 acaagcaaag ggaagaaatt tcaaaggatt tttacatcac gttaataagg gtagatacca
      541 aaagaatgtc ttaaagttaa gggttaaaaa gaaacagata agaacattga catcagagga
      601 agttaagcaa attattgatg cttgtcatac gaaaagagat aaattaattt taatgcttat
      661 gtatgagggt ggtttaagaa tcggtgaagt gttatcgctt aggcttgaag atattgccac
      721 ttgggacaat caaatccatt taacacctag agatgttaat gtaaatgaag cttatattaa
      781 attaaggaag gaaagaacaa tacatgtgag taaagaactg atgtcacttt atacagatta
      841 cttggtatat gagtatagtg aggaattgga gcatgattat gtttttattt ccttaaaaga
      901 aggctatttt gggaaaccac taaagtacca aagtgttctt gatctagtta gaagaatagt
      961 taaaaggact ggaatagaat ttacatcgca tatgcttcgc cacactcacg caacgcagct
     1021 aattagggaa ggatgggatg ttgcgttcat tcaaaagaga ttaggtcacg cacatgttca
     1081 gacaacgtta aatacctatg ttcatctttc agatcaggat atgaaaaatg agtttaataa
     1141 ataccttgag agaaaggagc ataagaaatg aatgcttcta gtaaaaggaa aattatcagt
     1201 cagagtgaga tcagcaaaaa aatagctgta atgaatgaag aaatgcaagg gttttgggct
     1261 aataatagtt gggatataag aaaatgtcca catccttctg ccatagaatt aagtaagaat
     1321 cctgctttaa gaaatcgttg ggttcgtttt gaacgtgtta aaaatctgtg gttaagaaca
     1381 gaattgaaat atttttattt ttaccattta aacaatggaa tatggaatgc aaaaactgtc
     1441 tggattagaa aaggaacagt aattaataaa atgttagatt ttttagattt aaagtatccc
     1501 agcattactt caattactga agttcctatt gaaaaagcaa tgacggagta tagaacatat
     1561 ttgacaaaac agggtgttag aattactact actaattata agattactgc taatcaagaa
     1621 aaaacacctg taaaagctaa ttcttactat gttactaatc taaaacaatt tatggagttt
     1681 tatgaggact tttattttga tggggaggag tgggataaag acatttggga tagacgtaag
     1741 ttacctttgc cagatgataa ggttaatccg acacaatatg aatatgtaat taactttaaa
     1801 ggggttcgca atacgtattt taaacaactc gtaaaaagat attgtaaatt gagattaaat
     1861 acggatagct tttcctatgt atgtgatatc gcccaaaaac ttaaagagtt ctttaatttc
     1921 ttggacatga attttaaaca cgttcagaga ataaaccaat taacgagaat ggaaattgaa
     1981 gcatatttaa gcgaattaaa tatgatgggg ataaagccta gcacaataaa tgggaggatc
     2041 tctatattgg aaggactatt tagtaccctt ctaaggctag aatggaatga tattccttcc
     2101 aaggtgttaa tctatccaga agactatccc aaaataccga gagcaaaacc tcgttttata
     2161 gatgaatacg ttctggagca attgaacagt catcttgata aattacccga atatatagct
     2221 acgatgacta tgatagtcca agaatgtggg atgaggataa gcgaattgtg caccttgaaa
     2281 aaaggttgtc tattagagga caaagatgga gattactttt taaagtatta tcaatggaaa
     2341 atgaaaaagg agcatatagt tccaatatca aaagaggtag ctttattgat taaagttcgg
     2401 gaagataaag tttcatggga gtttccagat agtgaatacc tctttccaag aaaagatgga
     2461 tcgccattaa aacaagaaac atttagagac gagttaaaca aattagctta tgagcaaaaa
     2521 atagtcgata aatcaggcga gatttataga ttccatgccc atgcctttcg ccatacagta
     2581 ggaacaagaa tgattaataa cggagtaccg cagcatattg tacaaaagtt tttgggtcat
     2641 gaaagtcctg aaatgacaag cagatatgct catatttttg atgaaaccct taaagctgaa
     2701 tttacaaaat tcaaagaaaa actggtaact aataatggag atataattga gttagataat
     2761 gacaatgaag tcgatgatgt tgatcttcaa tggttcaaga aaaatattaa tgcacaagtt
     2821 cttccaaatg gttattgccg attaccagta atagcaggtt cttgtccaca cgccaatgct
     2881 tgtttagatt gtactcattt ttgtacaagt aagcagtttt tatcgcagca tgaggaacag
     2941 ttaaatcgta ctgaggagtt attggcagtc gctaaggaaa agcaatggca aaggcaaata
     3001 gagactaata accgtgttaa agagcgacta gagcaaataa ttggcagcct gaaggggatt
     3061 agttagatgg ttaaacaggt acgaaataca gaggaaattg tgcgtttggc aaagcaaaag
     3121 tccaggcgga ctagagaaaa tgtagacaag gttatctcta aactatcgct tgaaggtaag
     3181 actattaatt ttaacacagt tgcaaaggaa gctaatgttt ctaaatcatg gctttataag
     3241 gaacatgata ttaggcaaag gattgaatct ctccgaaaac agcaaaaaac agaaaatgta
     3301 atatcaaaac ccaagaaaag ctcccgttct gaagaagtcc ttattaaaac cttaaaaaca
     3361 agagtaaagg aattagaaga ggaaaatata agattaagga accagatcca aaaattatac
     3421 ggagatttgt acattagaga atagttaatc tatgagtttc accaacttgt gctttgaact
     3481 ttgaagtgtt aaaattaata tactattaat cgaaaggaga gatgggtgga caatgaagaa
     3541 ctaatctaat ttttaacaaa tgaaatagta tatttcagtt taaaaactta ggattagtct
     3601 gcccattttt ccatatacta aataaatgta agactgaata gctaaaaagg ctaaatagtt
     3661 ttatttgagt ccttaaaaag tgacatgact aatccttcca acattatttg ggaggatttt
     3721 ttatttctcc ctgaatcaaa ataaagaaag aaggaatcga attgttaaaa caaaaggaac
     3781 taatggcaag ggttaaggaa cttgtccagt cagatgaacg aatatctgct tgtatgatgt
     3841 atggctcttt tacaaaagga gagggagatc aatactctga tatagaatat tacgtttttc
     3901 taaaagatga tacaatttcc acctttgatt cagcaaaatg gctaaatgaa gtcgcttcct
     3961 acactttact ctatcaaaat gagtacggta cggaagtagt aatttttgaa aatctaatac
     4021 gtggtgaatt tcatttcctt tccgaaaacg aaatgaatat tattccttca ttcaaagaat
     4081 caggctacat tcctgacaca aaagcaatgt ttatttatga tgaaacagga caactagaat
     4141 tgtatttatc agagttggaa ggtccgggac caaatagact tacagaagaa aacgtaaatt
     4201 ttttattgaa taatttttcc aacctatggt taatggggat taatgttctt aaaagagggg
     4261 aaaatgcacg ttcactggaa cttttatctc aattacaaaa aaatatacta caactcattc
     4321 gaattgcgga agaaaatgcc gataattggt ttaatatgac aaagaatctt gaaaaagaaa
     4381 ttagtcctga aaactatgaa aagtttaaaa agactactgc ccgattaaat gaattagaac
     4441 tatatgaagc ctataagaac tctttgcttc tcgttatgga acttcgaaat ctcgttgaaa
     4501 aacagtatca attaaccatt agcgatgatt ttttaggcaa actgtttaat tatatgaacg
     4561 aataaataaa aagcctgtat ctcaagtgat aactaagata caggctttaa ttgttattta
     4621 ctttaaatac atgttctcaa agtgaaagta ttgattttaa tggattttta atagtttact
     4681 cttgataaga tataattcca tcgcagcagg atatgaattt taccaaaagg atacaaaagt
     4741 gtggagaaat gatggggatc actgtactgg accacttgat catcggacgc aaacgatatt
     4801 ttagtttgag agaagaagga atgatggaag agaagtaagt tgctttcttg caaaaaaaat
     4861 gaagcaagtg tacaattata ttgtaatttt gtttgggaat agaagttgaa acatctgttg
     4921 cacctctatc gtacacgagc aatgttacaa tttaaattag tgcttttggc acgaggagga
     4981 tattattatg gaaaacggaa cagtaaaatg gttcaacgct gaaaaaggat ttggatttat
     5041 ctcacgtgaa gatggaagcg acgtattcgt tcacttctca gctatccaag gtgacggatt
     5101 caaaacatta gaagaaggtc aagcagtgac tttcgacgtt gaagaatcag accgcggccc
     5161 acaagctgca aacgttgtaa aagcttaatt ttatctgtaa atcaaattga tggtagacat
     5221 caatttttac gagagaacaa agcgagtaac aaaaatacct gccacaagag acaatttctc
     5281 ttgtggcagg t
//
LOCUS       DAYYHB010000134         5243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000134 DAYYHB010000000
VERSION     DAYYHB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.137"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..1152)
                     /gene="nagA"
                     /locus_tag="ACHA6B_002566"
     CDS             complement(4..1152)
                     /gene="nagA"
                     /locus_tag="ACHA6B_002566"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8950101.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1228..2064)
                     /locus_tag="ACHA6B_002567"
     CDS             complement(1228..2064)
                     /locus_tag="ACHA6B_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950102.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2248..3465)
                     /locus_tag="ACHA6B_002568"
     CDS             complement(2248..3465)
                     /locus_tag="ACHA6B_002568"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8950103.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3614..3775)
                     /locus_tag="ACHA6B_002569"
     CDS             complement(3614..3775)
                     /locus_tag="ACHA6B_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950104.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3848..3931
                     /locus_tag="ACHA6B_002570"
     tRNA            3848..3931
                     /locus_tag="ACHA6B_002570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3882..3884,aa:Leu,seq:gag)
     gene            complement(4171..>5243)
                     /locus_tag="ACHA6B_002571"
     CDS             complement(4171..>5243)
                     /locus_tag="ACHA6B_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950105.1"
                     /translation="VFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFS
                     LLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRY
                     NYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP
                     FFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNG
                     NKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTS
                     IFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKN
                     EFMQFGLTVLGICTFIITVINR"
BASE COUNT         1622 a         1073 c          832 g         1716 t
ORIGIN      
        1 atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg
       61 gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat
      121 tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa
      181 tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt
      241 gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta
      301 atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg
      361 tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat
      421 caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg
      481 attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc
      541 tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc
      601 agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt
      661 gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa
      721 ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat
      781 tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc
      841 ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga
      901 taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt
      961 catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc
     1021 aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt
     1081 aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat
     1141 aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga
     1201 gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg
     1261 tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct
     1321 tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat
     1381 tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc
     1441 ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag
     1501 cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata
     1561 tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt
     1621 ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg
     1681 caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata
     1741 cagattttca gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt
     1801 ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga
     1861 gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa
     1921 tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc
     1981 atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc
     2041 aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta
     2101 ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata
     2161 ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc
     2221 tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct
     2281 cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg
     2341 atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa
     2401 aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg
     2461 gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc
     2521 attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac
     2581 actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa
     2641 acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc
     2701 acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt
     2761 cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc
     2821 catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac
     2881 ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt
     2941 aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg
     3001 ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat
     3061 agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag
     3121 caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt
     3181 gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga
     3241 aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg
     3301 aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa
     3361 aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc
     3421 gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc
     3481 tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact
     3541 ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac
     3601 aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg
     3661 caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa
     3721 tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa
     3781 taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta
     3841 ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta
     3901 gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca
     3961 cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac
     4021 aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc
     4081 tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac
     4141 ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt
     4201 acaaatacca agcacggtta atccaaattg cataaattca tttttttgta cactcatgct
     4261 tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata
     4321 gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta accttgaaca
     4381 aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa
     4441 ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt
     4501 acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact
     4561 actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt
     4621 attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata
     4681 atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca ataaaaataa
     4741 gatattcatg tctaaaaaaa acggctttcc aaatgatgaa atacctaaat ctaacttatt
     4801 aagaaagttg aaatttttat attcaggaaa ccatttatta atttcatgtg cagcacgtaa
     4861 tgcattccct ggagttgtca aagagaaaat taattctgct aagcttatta ttgaaaatac
     4921 tgataactta taattatatc gtccattgac aatcaaatat aaactaacta tacttgttag
     4981 aacaaaaaaa caggcgttga cttgttcttg attagttaaa aatatcaata aaggaatact
     5041 tacccaatat atttttctat ttatttcttc tcctcttaag agttgaaaaa acggataaaa
     5101 agttagtaaa ctaaatgcta ctggccactg atagttcaag gtggttgctc tccaaccggt
     5161 ttcccccatt actgtcaatg gtattaaaca gtatatagag caaaccaata ataaattttt
     5221 aacatttatt ttctcaaaaa cat
//
LOCUS       DAYYHB010000135         5109 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000135 DAYYHB010000000
VERSION     DAYYHB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.138"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..2077
                     /gene="tdc"
                     /locus_tag="ACHA6B_002572"
     CDS             242..2077
                     /gene="tdc"
                     /locus_tag="ACHA6B_002572"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8950106.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2382..3860
                     /locus_tag="ACHA6B_002573"
     CDS             2382..3860
                     /locus_tag="ACHA6B_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8950107.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3826..>5109
                     /locus_tag="ACHA6B_002574"
     CDS             3826..>5109
                     /locus_tag="ACHA6B_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8950108.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFV"
BASE COUNT         1505 a          840 c         1097 g         1667 t
ORIGIN      
        1 tttgtttgtt cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt
       61 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
      121 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      181 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      241 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      301 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      361 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      421 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      481 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      541 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      601 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      661 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      721 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      781 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      841 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      901 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      961 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
     1021 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1081 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1141 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1201 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1261 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1321 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1381 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1441 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1501 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1561 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1621 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1681 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1741 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1801 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1861 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1921 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1981 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     2041 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2101 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2161 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2221 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2281 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2341 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2401 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2461 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2521 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2581 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2641 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2701 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2761 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2821 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2881 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2941 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     3001 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3061 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3121 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3181 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3241 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3301 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3361 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3421 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3481 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3541 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3601 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3661 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3721 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3781 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3841 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3901 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3961 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     4021 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4081 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4141 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4201 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4261 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4321 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4381 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4441 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4501 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4561 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4621 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4681 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4741 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4801 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4861 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4921 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4981 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     5041 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5101 tggtttgta
//
LOCUS       DAYYHB010000136         5099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000136 DAYYHB010000000
VERSION     DAYYHB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.139"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..833)
                     /locus_tag="ACHA6B_002575"
     CDS             complement(<1..833)
                     /locus_tag="ACHA6B_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8950109.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(847..1644)
                     /locus_tag="ACHA6B_002576"
     CDS             complement(847..1644)
                     /locus_tag="ACHA6B_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8950110.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1677..1961)
                     /locus_tag="ACHA6B_002577"
     CDS             complement(1677..1961)
                     /locus_tag="ACHA6B_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8950111.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1958..2959)
                     /locus_tag="ACHA6B_002578"
     CDS             complement(1958..2959)
                     /locus_tag="ACHA6B_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8950112.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="ACHA6B_002579"
     CDS             complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="ACHA6B_002579"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8950113.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4027..4875
                     /locus_tag="ACHA6B_002580"
     CDS             4027..4875
                     /locus_tag="ACHA6B_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8950114.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1534 a         1099 c          838 g         1628 t
ORIGIN      
        1 taaaatccgt catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg
       61 gcaggctgta cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca
      121 aaaatggcat aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa
      181 gcaaggaaga aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata
      241 ccgattgccg ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct
      301 aaagctttga ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc
      361 ggaacgaata ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct
      421 tttgtattca aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa
      481 aaacgaaact gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct
      541 gcttctggcg acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat
      601 ggttcagctg gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg
      661 gctgtaacaa tcatcgcttg tgcaacttcc cccatgatca acccgacgac taatccagtc
      721 atcaaaggag aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc
      781 gcggctaccc ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct
      841 cctccttcag atctttctga tataaccttc cagttccatc attgactgta tcagtgattt
      901 ctgtgttacc tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat
      961 tttatctatt atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag
     1021 ttctaattcc cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc
     1081 cagttgatag aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg
     1141 aatatattta ctgatagcgt catgcatagc atgtgctgcc acatttcttg cgtatttatc
     1201 tcctaatcct ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc
     1261 cgatgtcagt tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc
     1321 ttctttgcag atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa
     1381 atcttgttct aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc
     1441 aatgaaatat ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc
     1501 ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc
     1561 aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga aatcgattct
     1621 tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac
     1681 gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat
     1741 tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta
     1801 ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat
     1861 gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg
     1921 cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct
     1981 ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac
     2041 atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca
     2101 ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat
     2161 ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa
     2221 ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt
     2281 tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg
     2341 attgttccag taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat
     2401 ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga
     2461 ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc
     2521 taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg
     2581 tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt
     2641 caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa
     2701 tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa
     2761 aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact
     2821 ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg
     2881 actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg
     2941 ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca
     3001 tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc
     3061 atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc
     3121 gtccgttttg cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt
     3181 tcgtttaact gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc
     3241 caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat
     3301 ccttcaccga ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag
     3361 tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat
     3421 aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt
     3481 cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga
     3541 aactcttctg ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg
     3601 tctcctggtc cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca
     3661 ggaagcatta accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct
     3721 tgataagcag gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt
     3781 ccttgtactt tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc
     3841 aaaccaatta caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa
     3901 aaaatttttt tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc
     3961 cttgtatctt taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga
     4021 attctcatga gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca
     4081 aaagaacagg ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct
     4141 attagcaatt tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa
     4201 aaacttggtt ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat
     4261 ctatctgcta tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg
     4321 caaaaggtgt tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa
     4381 aaggatctaa aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt
     4441 gttggcggtt cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc
     4501 gcaacttctc acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa
     4561 aaagactgtg ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct
     4621 gaaaccgtaa gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc
     4681 cttgctaaac ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct
     4741 gaagctttag cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg
     4801 atgttttaca acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct
     4861 gaaatcaaag aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca
     4921 ctaccaaata tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag
     4981 tcggtgggtt agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga
     5041 gttacttgtt agatgcgata acaaagttag tatacagtag taaaaaattt atcttttct
//
LOCUS       DAYYHB010000137         5048 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000137 DAYYHB010000000
VERSION     DAYYHB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.140"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..1895)
                     /locus_tag="ACHA6B_002581"
     CDS             complement(129..1895)
                     /locus_tag="ACHA6B_002581"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8950115.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1855..3609)
                     /locus_tag="ACHA6B_002582"
     CDS             complement(1855..3609)
                     /locus_tag="ACHA6B_002582"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8950116.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3719..4288)
                     /locus_tag="ACHA6B_002583"
     CDS             complement(3719..4288)
                     /locus_tag="ACHA6B_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8950117.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4306..>5048)
                     /locus_tag="ACHA6B_002584"
     CDS             complement(4306..>5048)
                     /locus_tag="ACHA6B_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8950118.1"
                     /translation="LLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENIL
                     KDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEM
                     DYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIA
                     SLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYL
                     KNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1679 a          950 c          680 g         1739 t
ORIGIN      
        1 tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa
       61 attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc
      121 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
      181 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
      241 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
      301 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
      361 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
      421 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
      481 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
      541 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
      601 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
      661 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
      721 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
      781 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
      841 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
      901 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
      961 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
     1021 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
     1081 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
     1141 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
     1201 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
     1261 aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta
     1321 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
     1381 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
     1441 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
     1501 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
     1561 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
     1621 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
     1681 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
     1741 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
     1801 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
     1861 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
     1921 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
     1981 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
     2041 aactgttttt tcttttatca gatgttgaaa agccttttgt agttcatact cattatctgg
     2101 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
     2161 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
     2221 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
     2281 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
     2341 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
     2401 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
     2461 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
     2521 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
     2581 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
     2641 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
     2701 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
     2761 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
     2821 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
     2881 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
     2941 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
     3001 tccgcggata tattctaccg tttcagagct taacacatct aacgattctt gatatttttg
     3061 aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc
     3121 gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg
     3181 tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc
     3241 cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa
     3301 acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc
     3361 aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc
     3421 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
     3481 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
     3541 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
     3601 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
     3661 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
     3721 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
     3781 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
     3841 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
     3901 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
     3961 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
     4021 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
     4081 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
     4141 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
     4201 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
     4261 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
     4321 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
     4381 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
     4441 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
     4501 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
     4561 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
     4621 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
     4681 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
     4741 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
     4801 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
     4861 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
     4921 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
     4981 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
     5041 taacaact
//
LOCUS       DAYYHB010000138         5028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000138 DAYYHB010000000
VERSION     DAYYHB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.141"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA6B_002585"
     CDS             complement(<1..182)
                     /locus_tag="ACHA6B_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8950119.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA6B_002586"
     CDS             517..951
                     /locus_tag="ACHA6B_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8950120.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA6B_002587"
     CDS             974..1294
                     /locus_tag="ACHA6B_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950121.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA6B_002588"
     CDS             1435..1626
                     /locus_tag="ACHA6B_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950122.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA6B_002589"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA6B_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8950123.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA6B_002590"
     CDS             2112..2375
                     /locus_tag="ACHA6B_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950124.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA6B_002591"
     CDS             2399..2587
                     /locus_tag="ACHA6B_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950125.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA6B_002592"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA6B_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950126.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA6B_002593"
     CDS             3070..3441
                     /locus_tag="ACHA6B_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8950127.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA6B_002594"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA6B_002594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA6B_002595"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA6B_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950128.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA6B_002596"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA6B_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8950129.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1752 a          784 c          983 g         1509 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatat
//
LOCUS       DAYYHB010000139         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000139 DAYYHB010000000
VERSION     DAYYHB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.142"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHA6B_002597"
     CDS             85..972
                     /locus_tag="ACHA6B_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8950130.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA6B_002598"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA6B_002598"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8950131.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHA6B_002599"
     CDS             2274..2720
                     /locus_tag="ACHA6B_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8950132.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHA6B_002600"
     CDS             2993..4345
                     /locus_tag="ACHA6B_002600"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8950133.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHA6B_002601"
     CDS             4516..4803
                     /locus_tag="ACHA6B_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8950134.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYHB010000140         4925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000140 DAYYHB010000000
VERSION     DAYYHB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.143"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1116)
                     /gene="recA"
                     /locus_tag="ACHA6B_002602"
     CDS             complement(67..1116)
                     /gene="recA"
                     /locus_tag="ACHA6B_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8950135.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1225..2466)
                     /locus_tag="ACHA6B_002603"
     CDS             complement(1225..2466)
                     /locus_tag="ACHA6B_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8950136.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2706..3284)
                     /gene="pgsA"
                     /locus_tag="ACHA6B_002604"
     CDS             complement(2706..3284)
                     /gene="pgsA"
                     /locus_tag="ACHA6B_002604"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8950137.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3493..4416)
                     /locus_tag="ACHA6B_002605"
     CDS             complement(3493..4416)
                     /locus_tag="ACHA6B_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8950138.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         1328 a         1119 c          893 g         1585 t
ORIGIN      
        1 taaaaaaacg gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca
       61 taatttttat tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc
      121 gacttcttca ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc
      181 aggatgatta gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt
      241 ataagaatac caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag
      301 ttctccttct tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg
      361 aggtgctact ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc
      421 ttgtttcaat tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc
      481 gcgtcctcca ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg
      541 gttgatgaaa atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc
      601 ttgagacatc aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc
      661 tgctcgtgga actaaagcag caactgaatc gactactacg atatctacag ccccacttga
      721 tactaaagca tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag
      781 ttcatcgata ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc
      841 aatgaaagcg gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt
      901 tgttttacct gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc
      961 gacacctaaa gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc
     1021 gattttttcg cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag
     1081 tgcagcatct aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac
     1141 aaatcagagc aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa
     1201 aaaccgaaca aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa
     1261 tccttgcata accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc
     1321 cgaaaccacg ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc
     1381 taacgtgtcc ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc
     1441 acgtgcttga atggccatct gctccacaca ttctttgctg actgttccat attctgctaa
     1501 tagctccgga tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc
     1561 gcctggaaat acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc
     1621 tgtcaaactt tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac
     1681 ttttgctagc gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat
     1741 tttttcttct aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt
     1801 cagtcgtaat gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt
     1861 ctcaatcaga tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa
     1921 aactttagaa attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg
     1981 ttccacaaac attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt
     2041 atctgtttga taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc
     2101 ttcgataatc tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa
     2161 atatgccaat aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc
     2221 cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat
     2281 gagttcactt cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc
     2341 tacaactgtt tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt
     2401 ggcattcgta ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc
     2461 tttcattact tcccgctccc ttatatagta tacgctgtat ttttatattt ttttatttaa
     2521 aaagagggca aggagctgtg acaaaaatct tgtcacagct ccttgttcca aataaacagt
     2581 gttctcctgc caaaacctcg gtcataagtc acaatataca taaaacatga aaagctgttt
     2641 tttgaaatta tcccttgtga ctcagagtta gacggcaggc tcacaacctc tccactaaac
     2701 gatacttaca ttgatccttt gaacacaccg ctgtttttga tgaagtaatc cacacctgaa
     2761 tagatagtaa agatcagaca agcataaagc atgatttgat ctactggaat accgatcagg
     2821 ctgaatggga tattattgat aaacagcaag ataatagcaa ccatttgtgt agccgtcttg
     2881 acttttcccg gccaagctgc agccatgact tctccatgtt cgacaagtag taaacgcaag
     2941 cctgtgacgg ctaattcacg acagacaatg atcgctacta cccaaccagg ggcctttcct
     3001 tggtcaacca acatgataaa agcagtcata actagcattt tatctgccaa cgggtccgca
     3061 aacttgccaa aattagtgac aaggcctcgc gcacgggcaa tttttccatc taaccagtct
     3121 gtgatactag ctaaagcaaa aataactgca cctactagct gtgtgacagc cagtgtggtg
     3181 tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg
     3241 atcatacaaa ttctaagtac agttaattta tttggtaaat tcacactatc gactcctttc
     3301 ttccctattc tacgtgacta tcagcgaagt ttccaccgat attatttact tttccaaatg
     3361 aatttattca aaggagtatt tttattgaaa ttgatctttt tgaattaacg aagaaggacc
     3421 gcgacacaga tttgtcacga tccttctttt ttatacctac aagtaaacag atgcgtaaac
     3481 taatttatac gatcattctg cgtattgaag agtaaggttc acattttttg gactctgatt
     3541 gttttcccct ggattaactg gtacggattc tccattcgct ttgatcgttg cattgcttgc
     3601 tgcaccaatc gtgatggtcg catttgttgc tgtttccggt aacacggtac tctgtgtttc
     3661 atttgctgcc agtgtccctt gataaacata ggcattgtct actagcacac ctaaccatac
     3721 acgattgaca gcagtaaatt caagggttac aggcttggta gcgtctgtga tatttactgc
     3781 gatggcactg ccagaatctt ctcctgtagc tattgccatt ttcttttctt cacttgattt
     3841 tgttgttgat tctactgtag aagactcttt ggtctgcgtg ctttgctctg aggaagagga
     3901 ctcctcgact gatccttcta ctttaagcga agaagctgtg tctccgatta ttggatcagc
     3961 ttgacgatct tgccaagtca tatagcccac gacaacgaca atcgccaaag caaccaatcc
     4021 taataaaatg acaggaagac ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat
     4081 tgcttttcta gaagtactta ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa
     4141 tttgcttttc ccatcaaaaa catcgactaa atggtctcca tcttctccta cagcttcagc
     4201 gtattgccga ataaatgccc gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt
     4261 gtcgattgct tccaaataac gtttttgaat ttttgtaatc tgctgtaatt catctaaaga
     4321 tattttttta ttcaggcgtg cttgccgtaa tttttctccg atggtttcaa aagccactcg
     4381 ttcatcttct ccagtctgtt tgattctccg aagcatctgc tgttgcaacg aaagacgtta
     4441 tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt tttccgaata aacattgttc
     4501 tcctgccaac tcctcggtca caagtcacaa tatccaataa atataagaag ctgttttttg
     4561 aacttgttcc ttgtgactcg gagttaaacg gcaggctcac gacctctttc ttaaacggtg
     4621 ttcaatcagc tgttcctcgg tattagctca aaaaccggca aaatataagg agatattttt
     4681 ccgtatgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg
     4741 tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat taagaagctg
     4801 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttggtac
     4861 acggtgttcc agaagtagct tcttcggcaa taagccgaaa tatcacaaaa atttgagaag
     4921 caatt
//
LOCUS       DAYYHB010000141         4874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000141 DAYYHB010000000
VERSION     DAYYHB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.144"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..640)
                     /locus_tag="ACHA6B_002606"
     CDS             complement(98..640)
                     /locus_tag="ACHA6B_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8950139.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            775..1164
                     /locus_tag="ACHA6B_002607"
     CDS             775..1164
                     /locus_tag="ACHA6B_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950140.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1168..1932
                     /locus_tag="ACHA6B_002608"
     CDS             1168..1932
                     /locus_tag="ACHA6B_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8950141.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2061..3008
                     /locus_tag="ACHA6B_002609"
     CDS             2061..3008
                     /locus_tag="ACHA6B_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8950142.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3138..3878
                     /locus_tag="ACHA6B_002610"
     CDS             3138..3878
                     /locus_tag="ACHA6B_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950143.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4169..4837)
                     /locus_tag="ACHA6B_002611"
     CDS             complement(4169..4837)
                     /locus_tag="ACHA6B_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8950144.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT         1661 a          826 c          951 g         1436 t
ORIGIN      
        1 tgagtatatg tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa
       61 tttaatttag atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca
      121 cttttttagt ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac
      181 gctcttcgat ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta
      241 tttcgagaac tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac
      301 cgtgttctct cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg
      361 ttgtgaccat gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac
      421 ctactgcgta acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa
      481 caagggataa gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca
      541 gtcgatagcc tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat
      601 taatgaggaa gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt
      661 atataacaac aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca
      721 aaaaaatgat aaaatgatta taatagaatt ataaagatag gttaggtgat taaaatgaaa
      781 acggtcaatc gtgtgttttg tgccagccac gtgctaagtc tccttctcag tatatactta
      841 ggtgtctatt cgattcaaca gccaattgag gtagagaaca ttgtttttca gatgtcgctg
      901 acagatggtt taattttaag cgtttttttc tttttgatac tgtttattgg taatattttc
      961 ggtagcgttg ccagtttttt gaatttttct atttatccac tcctttcgct agcattagga
     1021 gtggtgggac tttgttctct gtgcttattt cccgagccat tcagtcctgc atttatatta
     1081 tttggtacac tgaacctgtt ccagacaact gttggagcat ggttgctatg gcgatcagga
     1141 aatttaatga aaataggtga gtaacaattg aatccaatta tattgaaacg ttctgtccat
     1201 catattccag tgttagaagt tgttctgaaa gaattaagag aggaacgtat tcccgttgtc
     1261 atctattatc acggatggca gacaagtaag gagttagttt tgacgcaagg aagaaaaatt
     1321 gcgcagcagg gcattcgtgt gatcctgcca gatgcgatga atcatgggga gcgcaaacag
     1381 ccggtttctt ctattccatc ctatacattt tgggatagta tctacggaaa tctgtttgaa
     1441 tttgacacac ttatcaagca tttagaaaaa agaggtcttc tacatgatca gcttgcagtt
     1501 ggcggggttt cgatgggggg aatgacaaca tgcgccttgt tggctaaaca tcctcatata
     1561 gtagcgggcg tgtgtttgat gggttcccct gcacctttgg aatatggaaa agaaatcgta
     1621 aggcgcgcat cggaatataa ttatgtcgta ccaaaagact attttgattt gatttcatgg
     1681 gttaagagct atgatttgtc attgcagccg gaaaagttag ctggacgtcc tctgttcttt
     1741 tggcatggac aagctgacga aaaaatccct tatatacaaa cggaacgatt tgtagaaaaa
     1801 aatcggcata agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac acagcgacat
     1861 atggtacaaa taccactaat ggaagaagct tccacttttt tagcaaaaaa tttgataaaa
     1921 aagtataaat aattgaaacg gaaaatcact catgacgata gaggattttc cgttttttat
     1981 tttgggctat aataaactct gttaggaaaa tttggtataa ttgatagtaa gaatgacaac
     2041 atgttagaaa ggaagagagc atgcccactt tagcagatgt tgcaaaacgt gccaatgttt
     2101 caaaaatgac ggtctctcga gtcatcaatc atccggaaca ggtgactgac gaattgaaag
     2161 agttagtttt ctcagcaatg gctgaactgg attaccgtcc aaatattgct gcaaaagcac
     2221 ttgtcacgaa tcggtcgcag atcatcaaat tatttatact ggaagaaatc gacacaactg
     2281 agccctatta catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa gcgcattatt
     2341 ctctccaatt agttacaaat gatgccttcg acgtcggttc ttgtgatgga tacatcatca
     2401 caggtgtcag aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca gttgtcttgt
     2461 ttggggaaaa ttctgaagga tatgattatg tagacagcga taatgaatat ggtacagcaa
     2521 aagcaacgca atatgcctta gaaagagatt atcaaacgat tatttatatc ggtatcgaag
     2581 tggacgagcc ttttgaaatt tcaagggaaa ctggttattt atctgtgatg aaggaagttg
     2641 atcttcctag taaaatcttg cggtttccaa atcattcgac agaagtagaa agatatatga
     2701 cagaaaactg ggcagatttt acagataaaa cttgttttgt atgtagttca gatcggctag
     2761 cactaggagc agtaagaggg attataaatg ctggaggaca cttgcccgaa gaatttggtg
     2821 tgattggatt tgatggggta tttttagacc aaatcagttc acctaaattg acaacgatca
     2881 agcaagatat tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa aaaatagaag
     2941 aaaaaggggc gagtcaaggt tatcgtcgtt tttttcctga actagttatc agagagacaa
     3001 cgaattaagt cttattacta tatatatatt tatgaaattc atcgctattt tcttaaaata
     3061 atcagcgcat tgctactgaa aacaatgaat ctgtgctatt attgagaaaa gttccatttt
     3121 tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt cctgcgtacg
     3181 atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg agtattattt gacggacaag
     3241 aagatctttt gactgaacag aatcagtccc cgtttgaaca gatgaaagaa tttcatttca
     3301 cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga ttgaagtcag
     3361 ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt accggattat accggccatt
     3421 accaatcaga aatcacatcg acaggacgaa aaaaactttt tggtgattcc tctcgttctg
     3481 tcagacaaag tgaaacaagg acagagtcca agcatactac acgaaacgca tctgttgaca
     3541 cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt acccgcatcg attatcccag
     3601 accctagaga aagcaaaatt tctgaggatc atttaatcga atcgatgaaa aaaacggatg
     3661 actcttatct gttgttcgat acagaaccga cgccgtatca agaaaaaaga aatggtgagc
     3721 cgtctgtacg aacattcaat cgaacaaaaa cgttagagga aacaaagcag tcagaagaaa
     3781 aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt acaaggattg atcgatgatc
     3841 aaggaaactc tttgggagaa aatagctatt ttaaataagt gatatacaaa tttatattcg
     3901 gctacagttg gaaaggaaac gctgcggcgt ttccttattt tatactctat ccgaaagttt
     3961 atatcgtatc ctcaaatttt tttgagttta agcgaatttt ttctcccaat aagtaagaaa
     4021 ataatggcaa aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa tagtttattt
     4081 ttttataaag aagattttac tgaataatat cattttagtt tacataatga tatttctgta
     4141 aattaaataa agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg
     4201 gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca
     4261 gatacttcac tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa
     4321 aatgtgtgag atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa
     4381 gacttttgcc aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc
     4441 cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg
     4501 tcatcaaact attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata
     4561 aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc
     4621 caaaaagaaa caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag
     4681 gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca
     4741 gcttagatgg aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga
     4801 ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa
     4861 attttcagca actt
//
LOCUS       DAYYHB010000142         4867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000142 DAYYHB010000000
VERSION     DAYYHB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.145"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1149)
                     /locus_tag="ACHA6B_002612"
     CDS             complement(424..1149)
                     /locus_tag="ACHA6B_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950145.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1303..1629)
                     /locus_tag="ACHA6B_002613"
     CDS             complement(1303..1629)
                     /locus_tag="ACHA6B_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950146.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1666..2031)
                     /locus_tag="ACHA6B_002614"
     CDS             complement(1666..2031)
                     /locus_tag="ACHA6B_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8950147.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(2068..2283)
                     /locus_tag="ACHA6B_002615"
     CDS             complement(2068..2283)
                     /locus_tag="ACHA6B_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950148.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2412..2747
                     /locus_tag="ACHA6B_002616"
     CDS             2412..2747
                     /locus_tag="ACHA6B_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950149.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2823..4820)
                     /locus_tag="ACHA6B_002617"
     CDS             complement(2823..4820)
                     /locus_tag="ACHA6B_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950150.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1537 a          945 c          630 g         1755 t
ORIGIN      
        1 gaaagaaact tattcttgct aataaactac gcgaaaggag tctaacgttg tttgatataa
       61 cttagactga tcgttaactt attttttata tttctacata gttatcttta gaaccatttc
      121 agatacatat gaaacaatta catatgattt tttcataaaa aaacagtact gtctagtagt
      181 ataaaatata caaatttctt aaaaattttt tcactaaaat cactacctga aacagtctaa
      241 aacacttata tatcaacgtt taatgaaact atctccgttt catttcagat acaaataaat
      301 atagtcgggc tccaaatccg aaaaaaaata cccccagtag cttatcctaa gctactggag
      361 atgtataagt acggtcatat aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa
      421 tcattattca ataacttttt taaatacttc gttgtaatca gttacctgtc cgttcttaaa
      481 taccacagaa taatcttttt tcccattttc aagtaaccga agtggaatat ctttaccaac
      541 tttctctgtc actgcattac tgatacctgt tagattagct aataaatctg ataaagattg
      601 agcatctgta gaaatctctg tatcagtatt tatcgtgatt cctttatctt catttacaag
      661 tttccaagta ggtggtatag ctgtgtatgt ttctacaaat gaattaagtt tttcattaag
      721 tgacttctct tttatatttc catctaaaaa ctctgtatat atggcttgag ctatactaaa
      781 acaaagctgg ccttgttcta ataattttac atcattagtt agtaaagctg tgttcacgtt
      841 atcaatcgct tcaagaagag tatctttggt gtccctaaca gctttaggtg tgccttctgg
      901 aatttcccta cttgatacct cgtccatttt atcttccata aaccccttaa aatcatatct
      961 ttctcccaaa tccatagaag tttttgagaa agctttcatt tctgagtcta tttcttcaat
     1021 aatatttaac atgtctgata catattgttt aggctgattc gtttttaatc tatatgtggt
     1081 aaatatctca attcctccaa ttatcaataa aattgccaaa caaatccata ataatttttt
     1141 ctttttcatt tttcctccta tacactgctg ttataacagt gactaatgta aagattttcc
     1201 cagcaatcat cagactatta tttacaggga atcaaaaaaa ctccttagta agcttacacc
     1261 tttaggagtt cctgtttgaa cggtatgcat aatacattta aatcaattta acattaacac
     1321 ctcagtatat ggtatctcta catatacacg gtcttttttt ggatagtact cgacatttat
     1381 attgatagca cttccattgt ctaacgtagc tttagcccgg tacgtgttat caccattatc
     1441 atgagtgatc gtcacatcat cacatgtccg atttaagtta tactgttctt gtaaaatctg
     1501 agttaccaat ggtacagatg acttctctag tccagatgaa cccgtcattc ttgaaaaaat
     1561 tatacctata atagccaata caacgatacc tataatgaca aacttcttat tcatatttac
     1621 tttattcaac tttttatctc ctatctccaa aatgattttc tctcatcagt aactcattga
     1681 catccccaat agtacaccaa agatggctcc agctacccct gctatcatca aaatcgtacc
     1741 catttgttca tctttctctc gatataagta acccataata acagcaacag caccaaatac
     1801 cacaggaata aatgctaatg atactaccat agaaatccaa ccaataatca aatacagctt
     1861 ccctgtctct tgtccatgta cgatttgttc cttcatttca ttaattgtct gctcagtact
     1921 tttctttact gtgtctgttc cggttgtctc atctgtttct tgtaatgctc cgcaaaactg
     1981 gcaatactta ctttcatttg ttatttcttt accacatttt ctacaataca tgtcttctcc
     2041 tcctaaaatc attcaattgc ttattagcta actgtttact ttattaaata catcagaaaa
     2101 cagctcattt attggtacac ttaatatctc agaaagcatt ttcatttgat agggtttagg
     2161 aatcgttctc cctgtttgcc acgaacttac acattgctgt gtaacattca tttttttggc
     2221 taactcagtt tgggaaagtc ctttttcttt tctgtatcgg ctgagattac ttactttttt
     2281 catgattctc ccctcctgtt ttattatata caacaatatg atgtatttca actcataaaa
     2341 tatataaata cttcaaatgt cattacaaat acaacaactt gttgtactct actgagaagg
     2401 ggggtgtatt aatgaaacag aagcaatatg caaaggtcat tagagaaaag cgaaaagcac
     2461 tcgggtggac acaaaaagaa ttagctaaaa aaatcttctc tacacaacag gcagtagcaa
     2521 gatgggaaaa ttctgtaaca gaacctaacc tcgatagctt aacagctctt tctagagccc
     2581 taggaacccc tgtaagccat tttctagaca atgttgacat agattatgag gaagagtttt
     2641 tggctctcta tcgctctctt agtactgaag atgcaattcg aacaattgac tatatgaagc
     2701 tattaaaaag gcaagaaaat gaacgcaatc agctattgaa agattgaaaa ataaataacc
     2761 caccgaactt taacggtggg tgtattgaga tgaaataaga ttaagagcta tggataacct
     2821 gtctagttta atataattgg gtctcttcta ttccttactc tccgtccatc atctcttgtt
     2881 acacttgtaa attgataaat aggaactaat tcaactcctg ataatatcaa ttcttttatt
     2941 acttcatcta tccctgcccc tttcgctaga tatactaaaa aactactgct tgctttcaca
     3001 ttgtatagtt tcttaatatc cttagaagct cgttcaagag tgaatccttg tttaacaaag
     3061 tagcctctgt tttctattaa agcatttttc acgctgttat ccctgacatt tcgtaggcgt
     3121 tcacaaacag tgttaataag ttctttttct gtcattagat atgtcccttg ccacgataag
     3181 ccctgtaaaa cttcatgtgc ttcatctaaa atcaattgtt tttccttgct aagttctgat
     3241 cgggaatttt gaggaaatac tcttttagcc tctttttcgt taaaacatat cttaacccat
     3301 tcataagaaa aatattgata tgtaaaatga aatttttcca gaagtggaag gatatcttca
     3361 ttcaattttt gaatcacaga agttgcatta aaaacgtcta atcgatagac attgcttctc
     3421 ttatgtttaa agtttcctaa cgctttctct tcctttaacc tatttaggta ttcgagcatc
     3481 ttcaaaggaa catcttcatc atctaatttt tttatcaatc ctataaaagt cataagtgat
     3541 agtaacttat tacatttatc tatgtcataa gttttaccat agtaagaact cattttttta
     3601 gctaattgtt cccccgacaa aatacaagtc acactttcct tactatcatc aaaaaaatta
     3661 acatcagaaa gcaagacatc atagacacgt cgcatttcct ctagataatt ccttattgtc
     3721 ctgaaaagtt tgtctttcat tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc
     3781 aattttcgaa aatgtgtagg atacaagtct aggttctcta atagccaatt aaatgtttct
     3841 acccgactgt ctttctttaa aattagcatc acaactccaa taaaattttc actgatatca
     3901 caattactgg aatgacaata aaaaatggaa tgcccagttt tactggtaaa aatactagca
     3961 gagggattat gatcgttgtg aaacagacat cttaagtttt tacttgactt aagtaattta
     4021 ttcatatcaa tggttaacaa tctttcataa atctcattaa aagacatgtc ggaggaatca
     4081 aacaggcagt ctttaaacca ctgtttcatt tcttctttgt cgtcattttt tatcaactta
     4141 acaaaatcgg tttcattgac tagttcatac tctctccctg aatcactttg acaattttta
     4201 gaacatgtca tggtaaaatc aatcggaagc tcagtaatat ctaacatgtt gtcttcatta
     4261 attattatca ctgcatctga cttatatcct ccaaaaaaca ttctattcga agcatttaca
     4321 ttgtcatcac acccggggat gttctttttc aagtattcgt aaacagcaac cattttacca
     4381 tagtcagtaa ttgcttcttt taatagaaag acaatcttgt atttttctag attttcagag
     4441 taccgaatac ttttttgtat cgcaaacgca gaattatgaa ctaaattgat agataaaacc
     4501 ttttctatgc ttaagtatct actatcatct ttactcaaat ctactacctt ccccttctca
     4561 tctttaacag tgttatcgac atccagaata attactctgg tttccacaaa actctccttg
     4621 tcattgcctt tattctttgt cttgcttgga ataaatgatt gtgatgaatt tagtatctct
     4681 acaaactcat ctcttgtaac ttctctatag ttatctaaac acctatctct tacatcagtt
     4741 ttttcttttt ttgtaactgg cttctctaat aatttttctt tatctacata aattggaata
     4801 ttaatcattg atgtttccat ttgtgtttcc tccttataaa attctgttaa agactaaaac
     4861 atacaga
//
LOCUS       DAYYHB010000143         4835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000143 DAYYHB010000000
VERSION     DAYYHB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.146"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..826
                     /locus_tag="ACHA6B_002618"
     CDS             <1..826
                     /locus_tag="ACHA6B_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8950151.1"
                     /translation="IYSSLSAPVFAGLFAGLVMGDLKAGLIIGGSMQLTVLGVGTFGG
                     ASKIDANSGTILATAFSVALGMNPEQAIAAIAVPVASLMIQLDILARFANTYFAHRID
                     KLVEDMNYKGIERNFLMGAIPWSFSRMIPVFLALAFGGGLVEKVVAVLNGDLKWLGDG
                     LSVAGAVLPAVGFAILLRYLPVKKHFPYLILGFTLTALLGTVFTNMQVLGTGVAGVVK
                     NFEGIFNSLPMLAIALIGFALAAISYKNGQMIPSSPAPKEVASNDSDEGEIEDDEI"
     gene            813..1622
                     /locus_tag="ACHA6B_002619"
     CDS             813..1622
                     /locus_tag="ACHA6B_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8950152.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEIMKTHTQFFNTSNFFNTIVTGIDLAIEEKEGIGGKDTVSGMKA
                     GLMGPFAAIGDSIFAALIPTIFGALAANMAINGNPTGIFIWIIAQIAVMIFRWKQLEF
                     AYREGISLVTTMQHRLTALTDAATLLGVFMVGALVATMINVKFSWAPSIGDVTLNMQN
                     NLDMILPRLLPAGIVAAIYWMLGKKNMTSTKAIFIVLVVCVALSALGVISK"
     gene            1660..2064
                     /locus_tag="ACHA6B_002620"
     CDS             1660..2064
                     /locus_tag="ACHA6B_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8950153.1"
                     /translation="MGKQLVLVSHGLFCEELKKSTEMIMGPQESIHTVSLLPAESAED
                     FRAKFEAVIADLDDFVVLADLMGGTPCNVVSRMILEGQAIELYAGMNMPMVIDFINSE
                     LIGTELALVESATSNICHVNERIIGDDDDEDE"
     gene            2208..2801
                     /locus_tag="ACHA6B_002621"
     CDS             2208..2801
                     /locus_tag="ACHA6B_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950154.1"
                     /translation="MDFNEKLKQLRQNKQWTQEELAERLFISRTAVSKWESGRGFPSI
                     DSLKAISQVFDISIDELLSNSELLSIAENEQQQQRKNFQHLLLGISDCMVGLLIVLPI
                     FGQAQGNHIAFVSLWQLSQETIIFKLIIFSFVILTMLFGIFELAFSRREQRIIGSVSL
                     ILSLFGVLLFIMTRHPYPATYVLMLLAVKGISLLNSR"
     gene            2910..3569
                     /locus_tag="ACHA6B_002622"
     CDS             2910..3569
                     /locus_tag="ACHA6B_002622"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8950155.1"
                     /translation="MKIAKKQLETAIGLLIVFILYTISLSLFDVQKIGPQHSEVGFST
                     INQYVFQKIGTSEFWYQVTEILGIFPLIIVGYFALRGFFQLYTRKKLLLVDKEILALG
                     FLYAGVAACYVLFEKVIINYRPILVDGQLEASYPSSHTFLAITVLLSAFYVLRKETGT
                     GKAIIAYVSLVVMIALVIGRFLSGAHWGTDIFGGILLGLTLASCYLAGIESSSKQKKE
                     R"
     gene            complement(3590..3662)
                     /locus_tag="ACHA6B_002623"
     tRNA            complement(3590..3662)
                     /locus_tag="ACHA6B_002623"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3627..3629),aa:Thr,seq:ggt)
     gene            complement(3780..>4835)
                     /locus_tag="ACHA6B_002624"
     CDS             complement(3780..>4835)
                     /locus_tag="ACHA6B_002624"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8950156.1"
                     /translation="AEHSYPEHHATKKSSTPATKTTTDLTNQQPIEPKNATSAGIEQL
                     LVEKNFSGTVLVFQHGQVILNKAYGWKDQARRIHNTIHTQYCIGSVQKGQTAYLIMQA
                     VKSGKISLEEKLSTFYPQISNSEKISIKDMLNMSSGLRIDEKKIAALNTTDPQKILSE
                     TIRQTTVAANSSYSYQPVNYVLLAGILEQVYHTDYSTLFDKLLRKPMKLKETSFYSET
                     NNQQALSYSESSSGPVYAPTTVSSYADELGTGNVFMSGEDLYSYFQDLYQDKFLTTQE
                     RAALWQSYPGEKYASGIYNMTNNYHSRGVKARFEALVDFSKDGQNGIILLSNVWTQPK
                     GYNDIVSKLYKQVVAGS"
BASE COUNT         1349 a          862 c         1101 g         1523 t
ORIGIN      
        1 aatttattca tcattgagtg caccagtctt tgccggatta tttgccggcc tagtaatggg
       61 cgatttaaaa gcaggactta ttattggcgg tagtatgcaa ttgaccgtac taggtgtcgg
      121 tacatttgga ggcgcatcaa aaattgacgc taactccgga acgattctag caacagcttt
      181 ttcagtagca ttgggaatga atccagagca agcgatcgct gcaattgcgg taccagttgc
      241 cagcttgatg attcaattag atattttggc tcgttttgcc aatacgtatt tcgctcatcg
      301 gattgataaa ttagtcgaag atatgaatta taaaggcatt gaacgcaact tcttgatggg
      361 ggccattcca tggtcatttt ctcgcatgat tccagtcttt ttagcgttag catttggcgg
      421 aggcttagtc gaaaaggttg ttgctgtctt gaatggtgat ttgaaatggt taggtgacgg
      481 tctatctgta gccggagcag ttttaccagc tgtcgggttt gcgattttat tacgttactt
      541 gcctgtgaaa aaacactttc cttacttgat cctagggttc acgttaacag cgttactagg
      601 aacagtcttt acgaatatgc aggttttagg cactggtgtt gcgggtgttg tgaagaattt
      661 cgaaggcatt ttcaattcac ttcctatgtt agccatcgca ttgatcggat ttgctttagc
      721 tgcgatcagt tataagaatg gacagatgat tccaagttcc ccagcaccga aagaagttgc
      781 ttccaatgat tcagatgaag gagagattga agatgacgaa atctaattat aagttgacga
      841 aggaagattt taagcagatc aatcgcagaa gcttgtttac cttccaatta ggctggaact
      901 atgaacggat gcaaggatca ggctatcttt atacaatctt gccgcaatta cgtaagattt
      961 atggcgatga cacgccggag ttaaaagaaa ttatgaagac tcatacacaa ttttttaata
     1021 cgtcaaactt cttcaataca atcgtgactg ggattgactt ggcgattgaa gaaaaagaag
     1081 ggatcggcgg aaaagacact gtatcaggga tgaaggctgg gttaatgggt ccctttgcgg
     1141 caattgggga ttcgatcttc gccgcattga ttccaacgat ttttggggcc ttggcagcga
     1201 atatggcgat caatggaaat ccaactggta tcttcatttg gatcatcgca caaatcgcgg
     1261 taatgatctt ccgttggaaa caactggaat tcgcttatcg tgaaggaatc tctttagtta
     1321 caacgatgca gcatcgctta actgctttga cagacgcggc gactttgctt ggggtcttca
     1381 tggttggggc gttagttgcc accatgatca atgtaaaatt ctcttgggca ccgagcattg
     1441 gtgatgtgac gttgaatatg cagaacaatc ttgatatgat cttaccgcgc ttattaccag
     1501 caggaatcgt tgcagcgatt tattggatgc taggtaaaaa gaacatgact tcaaccaagg
     1561 cgatctttat cgtattagta gtctgtgtgg cattatcagc attaggtgtg atttctaaat
     1621 aagttaggct gattccaatg aataatgagg agcgaaccaa tgggaaaaca attagtttta
     1681 gtgagtcatg gactcttttg tgaggaattg aaaaagagta cggaaatgat tatgggaccg
     1741 caagaaagca tccatacagt cagtttactt cccgcagagt cagcggaaga ttttcgcgca
     1801 aaatttgaag cagttattgc ggatttagat gattttgtcg tgctggctga tttaatgggc
     1861 gggacacctt gtaatgtagt ctcgcggatg attttagaag ggcaagcgat cgaactatac
     1921 gcaggaatga atatgccgat ggtgattgat ttcatcaata gtgaattgat cggtacagag
     1981 cttgcattag tcgaatctgc gacaagtaat atttgccacg tgaatgaacg gatcatcggt
     2041 gacgatgatg acgaggacga gtaaaatctc atagaagatg atatagaggc gtcgtttatt
     2101 tgtgaatagg agctagtttg aaagtctaaa agactttcga accagctcct ttttctgttc
     2161 tgctattatg gtatgattga ggagaaaaga aagaatggga gattagagtg gatttcaacg
     2221 agaaactaaa gcaattacgt caaaataagc aatggacgca ggaagaatta gcagagagac
     2281 tatttatctc acgaacagca gtttccaaat gggagtcagg tcgcgggttt cccagtattg
     2341 attccttaaa agctatttcg caggtttttg atatttctat tgatgagttg ctatccaata
     2401 gtgaattgct gtcaattgca gaaaatgagc agcaacagca gcggaaaaac tttcaacatt
     2461 tgcttttagg tatttcagat tgcatggtcg gcttattaat tgttctgccg atttttggtc
     2521 aagcacaagg gaaccatatt gcgtttgtct ctttatggca actttcgcag gaaacaatta
     2581 tcttcaagct tattattttt tcattcgtta ttttaacaat gctgttcggc atttttgaac
     2641 tcgctttttc taggcgagaa caacgaatta ttggatcggt ttctttgatt ttatcgttgt
     2701 tcggcgtact gcttttcatc atgactagac atccataccc ggcaacgtat gtattaatgc
     2761 ttttggcagt gaaggggatt tccctattaa atagccgttg atacgcatcg tcacatcaag
     2821 gtgacggtgc gcttcttttt gtttctggca gtctctgata cgttttattt ggatagcttc
     2881 caaataattg ttaggagagg attagtcaaa tgaaaatagc aaagaagcaa ttagaaacag
     2941 ccataggatt attgatcgtc tttattttat acacgatttc tttatccttg ttcgatgtac
     3001 agaaaatagg tccgcagcat tcggaggtgg gcttttctac aattaatcaa tacgtatttc
     3061 agaagatagg tactagtgaa ttttggtatc aagtgacgga aatcttaggg atttttccat
     3121 tgataatcgt ggggtatttt gctttacgag gatttttcca attgtatacc cgcaagaagc
     3181 tcttactagt ggacaaggaa attcttgcgc taggatttct ttatgctgga gtcgccgctt
     3241 gctatgttct ttttgaaaaa gtcatcatca attatcgacc gattttagtg gatggacagc
     3301 tagaggcatc ctatccgtca agtcatactt ttctggcaat tacagtctta ctgtcagctt
     3361 tttatgtatt aagaaaggaa acaggaacgg gaaaagcaat catcgcctat gtgagcctgg
     3421 tggtcatgat tgcgctggtt atcggacgtt ttttgtctgg agcccattgg ggaaccgata
     3481 tttttggtgg aattttatta ggtctgacac ttgccagctg ctaccttgca ggaattgaaa
     3541 gtagttctaa acaaaaaaaa gaacgttgaa aactcaacgt tcttagaaga gccacctgtc
     3601 gggcttgaac cgacgacctc ttccttacca tggaaacgct ctaccagctg agctaaggcg
     3661 gcatagttat ttaacacaag atatagaata gcgaaaacaa gcttatttgt caactggtta
     3721 aacagaaaaa aatgcggcat tagctgctgt gaattcttta tttataaacg agtcgtcgct
     3781 tacgagcctg cgacgacttg tttatatagt ttagagacga tatcattata tccctttggt
     3841 tgagtccaga catttgagag cagaataatg ccattttgac catcttttga aaaatcaaca
     3901 agggcttcaa aacgtgcctt aactcctcgt gaatggtagt tatttgtcat gttatagatt
     3961 ccgctggcgt atttttcacc tggatacgat tgccataatg cagcacgttc ttgtgttgtt
     4021 aaaaatttgt cctgataaag atcttgaaaa tagctgtaca aatcctcccc cgacataaac
     4081 acattgcctg ttcctaattc atctgcgtaa gaagatacgg tcgtaggagc gtaaacaggg
     4141 ccggaggaac tttctgagta tgaaagagcc tgctgattgt tcgtttctga atagaagctt
     4201 gtttctttca gcttcattgg ttttcttaaa agtttatcaa atagtgtgct gtagtcggta
     4261 tgatagactt gctccaaaat tccagctaac aaaacataat tgaccggttg ataggagtaa
     4321 ctagaattcg cagccacagt cgtttgacga attgtctctg aaagtatttt ttgtggatca
     4381 gtagtgttca aggcggcaat tttcttttcg tcgatacgta acccagaact catattcagc
     4441 atatctttga tagagatttt ctcactatta gaaatttgcg gataaaaggt gctgagcttt
     4501 tcctctaggg aaattttacc agatttaacc gcctgcatga tcaaataggc agtttgtccc
     4561 ttttgaacgg agccgatgca atattgtgta tgaatcgtat tgtggattct tctagcttga
     4621 tctttccagc cataagcttt attcaaaatc acctgtccat gctgaaaaac taaaacagta
     4681 ccgctaaaat ttttttctac caataactgc tcaataccag cggacgtagc attttttggt
     4741 tcgattggtt gctgattggt caaatcagta gttgttttcg ttgcaggtgt gctgcttttt
     4801 ttcgttgcgt gatgctcagg atagctgtgt tcagc
//
LOCUS       DAYYHB010000144         4750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000144 DAYYHB010000000
VERSION     DAYYHB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.147"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1101)
                     /locus_tag="ACHA6B_002625"
     CDS             complement(139..1101)
                     /locus_tag="ACHA6B_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8950157.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1103..2542)
                     /locus_tag="ACHA6B_002626"
     CDS             complement(1103..2542)
                     /locus_tag="ACHA6B_002626"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8950158.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2727..4673
                     /locus_tag="ACHA6B_002627"
     CDS             2727..4673
                     /locus_tag="ACHA6B_002627"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8950159.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1513 a          813 c          779 g         1645 t
ORIGIN      
        1 ggatttctcc actccaaaaa agtctaactt ttgggggtca ctacaagttg acaactcctt
       61 tttgtttaca aatcaattgg atatctacaa attgtatttt ctaacgaatt ttatagacat
      121 taaaaaatta atttaacgtt aaatacttcc tccagaaagt attgagattg gtaaaatata
      181 atccttattt gtcttttcgc ctttaatttt tttcagaaga atatcaacaa taagacttgc
      241 aatttcatca attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga
      301 ggtaccatcg tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc
      361 aattttcata gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg
      421 atttataata gttgacttta tttccatttc acgacggata gtagacagat tattaggaat
      481 tttgaaaatt tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg
      541 agaatccgtg ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa
      601 agtcttagct gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg
      661 agctaaattt ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc
      721 aagattatgg cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta
      781 ttctctctct ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt
      841 aaataactct atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg
      901 aaaaataaga ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg
      961 tttataacca agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata
     1021 tcccttatta ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac
     1081 atcagttaat tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc
     1141 ctgatttgat ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt
     1201 tttctccttt attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg
     1261 tttctttagt atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc
     1321 ctgctttgct gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc
     1381 catttccttt actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta
     1441 gttcaagctc ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac
     1501 gtaatgatgt aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt
     1561 cttttacaag actcattgct ccctgataat catacttatc agttggataa tcgatatcag
     1621 gtagtccaat ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg
     1681 catacgcttc aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt
     1741 tttctatgtc aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta
     1801 gctcagattt atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata
     1861 ctagatttgg acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa
     1921 cttcctccca tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt
     1981 tttctaaatt ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg
     2041 gatcacgaaa atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg
     2101 taatgttgta gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt
     2161 catttcgaac gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac
     2221 cggaataagc gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat
     2281 gaaaatgaac taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc
     2341 caaatggcca attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag
     2401 gatcgtttaa caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt
     2461 ttacattatc cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc
     2521 gtatttctcg aggtaagttc atattttact ccttcaattt atctttttac aactagtatg
     2581 gtaaacgttt tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa
     2641 cgtttgacat atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac
     2701 gtaaaaataa aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac
     2761 aagcggtcgg aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg
     2821 tactaaaaga tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaaa
     2881 gcacatttaa aattaatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg
     2941 tttatgacga acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac
     3001 aaattgctag taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag
     3061 atatttttgt tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa
     3121 actttttaac agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga
     3181 ttgaaggtat ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa
     3241 ttttagtagg tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa
     3301 ttggtatgat catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg
     3361 gttatcatgt ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt
     3421 ggatattatc tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta
     3481 cattcacgcc attattatca gtaatgatta ctggattttt gactttcaca gttattggcc
     3541 ctgtaatgtt attgttgtct aatggaatta cagacattat tgtttggcta tataacacta
     3601 ctggatttat tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt
     3661 tacatcaatc atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg
     3721 gacatggaga ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa
     3781 catttgccat ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg
     3841 gtgtctcagc acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc
     3901 gctttccatt cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac
     3961 ttaaagtagt tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg
     4021 caacatctat tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa
     4081 ttacttactt ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg
     4141 aacaaacacc agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg
     4201 aatcaacaca aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga
     4261 ttagtctttc ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg
     4321 ccattaaacc aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt
     4381 ttgaaactgg acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata
     4441 ttggtattga tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc
     4501 aaaaagttag aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag
     4561 gtcttgatga tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata
     4621 tagtagctga aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt
     4681 tccctgagta aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt
     4741 tgagaataac
//
LOCUS       DAYYHB010000145         4657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000145 DAYYHB010000000
VERSION     DAYYHB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.149"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..219)
                     /locus_tag="ACHA6B_002628"
     CDS             complement(<1..219)
                     /locus_tag="ACHA6B_002628"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8950160.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="ACHA6B_002629"
     CDS             complement(330..584)
                     /locus_tag="ACHA6B_002629"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950161.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /gene="prgO"
                     /locus_tag="ACHA6B_002630"
     CDS             complement(777..1052)
                     /gene="prgO"
                     /locus_tag="ACHA6B_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8950162.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="ACHA6B_002631"
     CDS             complement(1024..1977)
                     /locus_tag="ACHA6B_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8950163.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="ACHA6B_002632"
     CDS             2589..4082
                     /locus_tag="ACHA6B_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8950164.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="ACHA6B_002633"
     CDS             4217..4513
                     /locus_tag="ACHA6B_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8950165.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..>4657)
                     /locus_tag="ACHA6B_002634"
     CDS             complement(4537..>4657)
                     /locus_tag="ACHA6B_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950166.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1526 a          772 c          811 g         1548 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaa
//
LOCUS       DAYYHB010000146         4618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000146 DAYYHB010000000
VERSION     DAYYHB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.151"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..957
                     /gene="vanY"
                     /locus_tag="ACHA6B_002635"
     CDS             151..957
                     /gene="vanY"
                     /locus_tag="ACHA6B_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8950167.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            975..1682
                     /locus_tag="ACHA6B_002636"
     CDS             975..1682
                     /locus_tag="ACHA6B_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8950168.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1679..2650
                     /locus_tag="ACHA6B_002637"
     CDS             1679..2650
                     /locus_tag="ACHA6B_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8950169.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2643..3671
                     /gene="vanB"
                     /locus_tag="ACHA6B_002638"
     CDS             2643..3671
                     /gene="vanB"
                     /locus_tag="ACHA6B_002638"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8950170.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3677..4285
                     /locus_tag="ACHA6B_002639"
     CDS             3677..4285
                     /locus_tag="ACHA6B_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8950171.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4351..4524)
                     /locus_tag="ACHA6B_002640"
     CDS             complement(4351..4524)
                     /locus_tag="ACHA6B_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950172.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1299 a          972 c         1230 g         1117 t
ORIGIN      
        1 tttgaccgac aggtataacc ctgccggtct ttttttgctg ctacaggaaa actacagact
       61 ggctacaggg aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt
      121 agttttattg caaaggaggc attcgatcaa atggaaaaaa gcaactatca ttccaatgcg
      181 gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc
      241 attatttcgt tcacagtctg cacgctgttt ttggggcgga gactggcttc cgtattggag
      301 gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag
      361 aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc
      421 ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa
      481 ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat
      541 gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa
      601 cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca
      661 caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt
      721 ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga
      781 tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca
      841 gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc
      901 acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa
      961 aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa
     1021 tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct
     1081 atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat
     1141 tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa
     1201 agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct
     1261 ggttgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt
     1321 ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga
     1381 ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg
     1441 atgagaaaat catcggtcag gtgttcgccg acaaagagcc ccaagcatta tacaaaatta
     1501 caaatggcag tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta
     1561 aacggatgca agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa
     1621 acaaatgcaa aatctgttat cccctcccgg aaagtgtgga tattcaggag gagaaccaat
     1681 gagaaaaagt gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa atgctttccg
     1741 cgctttatcg ccggattttc atattatccc tacgctgatt agcgatgcga tatcggcaga
     1801 caacgcaaaa ttggccgctg gcaatcaatg cgttagcgta agccataagt ccgaggtttc
     1861 cgaggcgaca attcttgcgc tgagaaaggt cggggtaaaa tacatttcta cccgcagcat
     1921 cggctgcgat cacattgata cgactgctgc cgagagaatg ggaatctcgg ttggcacggt
     1981 tgcgtattcg ccggacagcg ttgcggatta tgctttgatg ctgatgctga tggccatacg
     2041 gggtgcaaaa cccaccatgc acgccgtggc gcaacaagat ttcagattgg atcgtatccg
     2101 ggggaaagaa ctgggggata tgactgtggg agttattgga accggccata tcgggcaagc
     2161 ggtcgtcaaa aggctgcggg gatttggatg ccatgtgctg gcctatgata acagccgaaa
     2221 aatggatgca gattatgtcc agcttgatga gcttctaaaa aacagcgata ttgttacgct
     2281 ccatgtgccg ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa ttggagagat
     2341 gaagcaaggc gcatttttaa tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct
     2401 ggtggaggcg ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt tggagggcga
     2461 ggatcagttt gtttataccg attgctcgca gaaagtgctt gaccatccct ttttgtcgca
     2521 gcttttaagg atgccgaatg tgatcatcac accccatacg gcgtactaca ctgagcgtgt
     2581 gctgcaggat actacagaaa aaacaatcag gaattgtctc aattttgaaa ggagtttaca
     2641 gcatgaatag aataaaagtc gcaatcatct tcggcggttg ctcggaggaa catgatgtgt
     2701 cggtaaaagc cgcaatagaa attgctgcga acattgatac ggaaaaattc gatccgcact
     2761 acatcggaat tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt acggaatggg
     2821 aagccgacag tctccccgcc atactctccc cggataggaa aacgcatggg ctgcttgtca
     2881 tgaaagaaag cgaatacgaa acacggcgta ttgatgtggc tttcccggtt ttgcatggca
     2941 aatgcgggga ggatggtgcg atacaggggc tgtttgtatt gtctggtatc ccctatgtgg
     3001 gctgtgatat tcaaagctcc gcagcttgca tggacaaatc actggcctac attcttacaa
     3061 aaaatgcggg catcgccgtt cccgaatttc aaatgattga taaaggtgac aagccggagg
     3121 cgggtgcgct tacctaccct gtctttgtga agccggcacg gtcaggttcg tcctttggcg
     3181 taaccaaagt aaacggtacg gaagaactta acgctgcgat agaagcggca ggacaatatg
     3241 atggaaaaat cttaattgag caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg
     3301 ggaacgagga tgatttgatt gtcggcgaag tggatcaaat ccggctgagc cacggtatct
     3361 tccgcatcca tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg attacagttc
     3421 ccgcagacat tccggtcgag gaacgaaatc gggtgcagga aacggcaaag aaagtatatc
     3481 gggtgcttgg atgcagaggg cttgcccgtg ttgatctttt tttgcaggag gatggcggca
     3541 tcgttctaaa tgaggtcaat accctgcccg gttttacatc gtacagccgc tacccacgta
     3601 tgatggccgc cgcaggaatc acgcttcctg cactgattga cagcctgatt acattggcgt
     3661 taaagaggtg ataagtatgg aaaatggttt tttgttttta gatgaaatgt tgcatggcgt
     3721 ccgttgggat gccaagtacg ccacatggga taacttcaca ggaaaaccgg tggatgggta
     3781 tgaggtgaat cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat
     3841 ccatgcggct gcgcttggct atggcttgct tttatgggat ggatatcggc caagaactgc
     3901 ggtggactgc ttcctgcgtt gggcagcgca accggaggac aatctcacaa aagaaaaatt
     3961 ttaccccaat atagagcgag ccgagttgat tacaaagggt tatgtggcct cacaatccag
     4021 ccatagccgt ggaagcgcaa ttgatcttac gctctaccac ctggatacag gggaacttgt
     4081 ttcaatggga agtaacttcg attttatgga cgaacggtcg catcatacag caaaagggat
     4141 aggggatgca gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt
     4201 tcagtcttat cgctttgaat ggtggcacta taagttgatt gatgagccat accccgatac
     4261 ctattttaat tttgccgttt cataatgaaa gtattggttt tgtatcagat tttggatttg
     4321 ataaaatcta tgctggtcat ggacaatctt ttactggaaa gtggttaaag catcttttgt
     4381 aatacattgc aaattatagt attcaatctg cccagcggca gaaaagaaaa aaaccgctgt
     4441 tgggcagacc tttcccacgc ccattttgta ttttgctggc ggcggttttg agaaatcaag
     4501 gccgccgttc ttttctggca gcaaaaggtg tggcggccca taggaggatg ccgctatgct
     4561 gttttgctat tgccatgctc taatccactt ccaccactca aaaaaagcct tgcccctc
//
LOCUS       DAYYHB010000147         4421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000147 DAYYHB010000000
VERSION     DAYYHB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.152"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1462)
                     /locus_tag="ACHA6B_002641"
     CDS             complement(176..1462)
                     /locus_tag="ACHA6B_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8950173.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACHA6B_002642"
     CDS             complement(1455..2723)
                     /locus_tag="ACHA6B_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8950174.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACHA6B_002643"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACHA6B_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8950175.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="ACHA6B_002644"
     CDS             complement(3495..3941)
                     /locus_tag="ACHA6B_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8950176.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="ACHA6B_002645"
     CDS             complement(4034..4279)
                     /locus_tag="ACHA6B_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8950177.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         1273 a          895 c          765 g         1488 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg t
//
LOCUS       DAYYHB010000148         4416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000148 DAYYHB010000000
VERSION     DAYYHB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.153"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHA6B_002646"
     CDS             59..913
                     /locus_tag="ACHA6B_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8950178.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHA6B_002647"
     CDS             1010..3259
                     /locus_tag="ACHA6B_002647"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8950179.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACHA6B_002648"
     CDS             3262..>4416
                     /locus_tag="ACHA6B_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8950180.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAYYHB010000149         4395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000149 DAYYHB010000000
VERSION     DAYYHB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.154"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACHA6B_002649"
     CDS             <1..93
                     /locus_tag="ACHA6B_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8950181.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACHA6B_002650"
     CDS             complement(151..1371)
                     /locus_tag="ACHA6B_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8950182.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACHA6B_002651"
     CDS             complement(1391..2002)
                     /locus_tag="ACHA6B_002651"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8950183.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACHA6B_002652"
     CDS             complement(2045..2977)
                     /locus_tag="ACHA6B_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8950184.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3397..4257)
                     /locus_tag="ACHA6B_002653"
     CDS             complement(3397..4257)
                     /locus_tag="ACHA6B_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950185.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1256 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaacaaat aagggaaaca aacaacacat ttttgtgtgt
     3181 tgcttatttc cctctaacta tcgttttcta tttgatggtt aacctactta aaataaatac
     3241 tgcaacgaca aacggagatc aagcatgctt tgtaacgtat tttacgataa ttacgacttt
     3301 attttattac tctatcttgc tgccataccc gtctatccct aatcatcttt ggtgaatttt
     3361 ttcaaagtta gcataacaaa aataagtatt ttttccttag ttaatcaata gtatcatttt
     3421 tttcatatga tttaattcat caattaattt tttgttttta ttaattttag caaaagctag
     3481 tgcatcttga atcaattcga gcacttctgc cttcgactta tttttagcct gtgcgttctt
     3541 cgctaattgg gcagcagctc gtgctaagta ataagtcaaa tggttatccg aacaaatatc
     3601 gattgcgtaa tttagtagtg catcacttgt ttccaaatca ccgtcttgcg caaaaaaaac
     3661 accacaatga aagacgatat ttaacacccg ccagatatct tctgttgtac gaatcggata
     3721 cgtataaatt ttttcaaaca ctcgattgaa ataaacttct gctttttcca actctttttc
     3781 tctagcatag accattccaa tacctgtata tgccaataaa cgaaacaacg tgttggctgt
     3841 ttctgccatc aacaactgat caaaatggaa caacacatcc gtaattggtg cattcgtaaa
     3901 aatcatttca aatcctttta gatagtagta acgtgcaatc agctcagact cgtcaattac
     3961 cttgggatca atggctgcta aaatctgtgt caattgttca tattcactcg taatcagtga
     4021 aaactctgct ttttctagct gttcatcaag ttgtgtattg atcacgttca tcgaagaaaa
     4081 aagttcaact aacggaagat tgattcgatt acataatttg attaaaattt ttaacgaagg
     4141 gacttgacca ttgttctcaa atttacttaa tgttgcttgt gtacaaattc cttcagacac
     4201 ttctttttga gataagttgg cagcctttct tgccgtaata aatttttcaa tgttcattat
     4261 tttctctcca atcagacctc acacgaaaat catttctgtc tcgattttag catgttttct
     4321 ttgggaatgc tttaggttct gttgcaaagt tttaaataaa gaataaagtc ccttacggta
     4381 tctatgattt aagct
//
LOCUS       DAYYHB010000150         4392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000150 DAYYHB010000000
VERSION     DAYYHB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.155"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            969..1892
                     /locus_tag="ACHA6B_002654"
     CDS             969..1892
                     /locus_tag="ACHA6B_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8950186.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1908..2447
                     /locus_tag="ACHA6B_002655"
     CDS             1908..2447
                     /locus_tag="ACHA6B_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8950187.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3132..3305
                     /locus_tag="ACHA6B_002656"
     CDS             3132..3305
                     /locus_tag="ACHA6B_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950188.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3711..4145
                     /gene="bacA"
                     /locus_tag="ACHA6B_002657"
     CDS             3711..4145
                     /gene="bacA"
                     /locus_tag="ACHA6B_002657"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8950189.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1661 a          659 c          727 g         1345 t
ORIGIN      
        1 tgcaccaaat acggaggtat ttggctagca aatatagagc ttgccaaacc cttttagtga
       61 gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt ccgtaggacg
      121 tactgaaagg gtgggactta tgagcaaatt aatgcaaaat ttggctagca aaaaagctaa
      181 aaaatatatg ctaataatcg agcgaaataa acactttttc aagtgagcga atccgccatg
      241 acaaagctga agcagtttat ttcagctttg tcatggcgtg tgccgacttt tttaaaagtc
      301 taggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac gtttgaaacg
      361 cctaagtata taaatatata agaataggtt taagagtgct taaatcgaat tttagagggc
      421 atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc gttttaagaa
      481 caagataagg gatttatact aggactgtct taaaatgtct taaacgaacg ctcagagctt
      541 ctcaaagggt tccttatgaa cggcttttag caaaggagcc ggaggcgact gcgcagtata
      601 atgtgagagc agagcgatca caccttttta gcgaaccgta gggagcaagg tttggaattg
      661 gcagttttga taaattcgcg aacctgttta gagaaaaaag gagtgatttt ttaggcggga
      721 aaattagcct cccctctttt tatatctctt tttatatctc tttttatatc tcttttatgt
      781 cggctgtgtc cttactctcc caagcaattc agagtttttt caagtaggtt ttctacgctt
      841 ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttcc
      901 gacattattg agtattttta taaagtatgt tacttttatt tcatacttta taaaagtagg
      961 agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat attcctctga
     1021 gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt gtacgtgaaa
     1081 aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc ggttatacag
     1141 aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta ttaagtatta
     1201 atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca aaatatagca
     1261 ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa ggacttttta
     1321 atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg gatagcacat
     1381 attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg actgaatttt
     1441 ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat agtccgtcaa
     1501 atattgacaa atttatctta aggccaatta aagaagaact cactccctta tttaaaggat
     1561 taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt tatcgtttta
     1621 cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa gaagatttac
     1681 gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa aaatggcgcg
     1741 ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt aaacaacaac
     1801 aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc aaacaagatt
     1861 tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg gcagataaaa
     1921 caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac aaaaaagttt
     1981 ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg attaacgaaa
     2041 ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta aatcgaaaac
     2101 tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt tccgaattaa
     2161 aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta caagaaaaag
     2221 ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc actctacaag
     2281 cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca gaagaaactc
     2341 caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa ccacttattg
     2401 aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat tttctagaag
     2461 ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac tcaaattttt
     2521 atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt gtaaactatc
     2581 aaactatatg gtgacttatt gcttaaaata ggttgaaatc tcactcaatt tttggtggaa
     2641 aaacaaaata aattctaatc gattttgttg aaaaagtcat aaaaaaacaa atgtgatttt
     2701 aacaaaattt caaaaaaatc gtttgaaatt tcgtttattt tttttgagaa ttttgatcat
     2761 cgtagggtca ttgtagaatc attatgagat catcgtggga tcatcgaata aagtatggga
     2821 aaggcttgcg gtgcttggta ttaagggcat tctccgcctc ccaggttctt gaccattttc
     2881 tctcaacgtg gtaaaaatcc cacttataag agaaaatggt caagaaccta aacggtcggc
     2941 gaagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa
     3001 attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa tttaaacatt
     3061 tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg
     3121 aggtataacc tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat
     3181 taggttttag ccccatgcta ttatccgtaa ttggaaaaaa caattccaac ggagaactta
     3241 aattaaattc aaattattac aacagtccta acgaagaatt gtttgtaaaa gagacggata
     3301 aataataaaa aagactgctc tttcgagcag ccagttaaaa aaataatgag agtttattct
     3361 tgaaaaagca acgcgcttat aaagcgctac caaaatttta tcatatttta taaaaaataa
     3421 caatccagta aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactacaga
     3481 aaatcaagcc ttaaaaagag ctttttacct agaccaatta aaaatcacaa agtgagcgtc
     3541 tctatccatt ggtagagggg caacttggtc tagtcgacat tatgtctact aacctttaaa
     3601 ataacacgtt ttataaaata aaccctcttg acaatattta attttctgat tattctaaat
     3661 atatacagaa agcgctttcc taggatttta tatctaagga ggaagtaaca atgaaaaata
     3721 aaattattaa aattattact tgtctatttt tgttcggtat gattgttgga gcaagtactc
     3781 cgttaaatct gtttcctttt agtgctagag gtataaaagt ggaagctgca agttcacgat
     3841 ataatcataa tcatcgtgga tttacttgct ataaaatgaa ctattatgtt actaataaaa
     3901 tgtgtagaga tttgaaaaaa aattataata agctaaagac tcctgctcaa attgcatctt
     3961 ttattcctat tggtggagtt ggaacatggg ttattacaaa tgcttttagt agtgctgttg
     4021 atgcaatgaa tgtctttgta agagctgcaa atcaaggaaa aggtgtacaa ttaacatata
     4081 atgctcactt tagcaataca acatcatatc aatacaatga ttacgctcgt tatgtaataa
     4141 aataatcaga aagtgaaact atctcatgaa taaaaagatt ttagctgtaa ttatttcaat
     4201 aatagtttta atcactatgt tcttcatatt taggctaatt ttcaatataa gcttacaaaa
     4261 aagtattctt tatctaattc ctatagcatt tatcttagca atttttagaa gtatttacgg
     4321 aaataaaaac tctaaatagc taaaaataaa tcatactaaa ttttacaaaa agtggctagc
     4381 attttctagc ca
//
LOCUS       DAYYHB010000151         4377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000151 DAYYHB010000000
VERSION     DAYYHB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.156"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACHA6B_002658"
     CDS             73..234
                     /locus_tag="ACHA6B_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8950190.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACHA6B_002659"
     CDS             253..1530
                     /locus_tag="ACHA6B_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8950191.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACHA6B_002660"
     CDS             1540..2196
                     /locus_tag="ACHA6B_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8950192.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACHA6B_002661"
     CDS             2196..3572
                     /locus_tag="ACHA6B_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8950193.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACHA6B_002662"
     CDS             3702..4115
                     /locus_tag="ACHA6B_002662"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950194.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACHA6B_002663"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACHA6B_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAYYHB010000152         4341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000152 DAYYHB010000000
VERSION     DAYYHB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.157"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            440..586
                     /locus_tag="ACHA6B_002664"
                     /pseudo
     CDS             440..586
                     /locus_tag="ACHA6B_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(674..967)
                     /locus_tag="ACHA6B_002665"
     CDS             complement(674..967)
                     /locus_tag="ACHA6B_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8950195.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1004..1189
                     /locus_tag="ACHA6B_002666"
     CDS             1004..1189
                     /locus_tag="ACHA6B_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950196.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1392..2570)
                     /locus_tag="ACHA6B_002667"
     CDS             complement(1392..2570)
                     /locus_tag="ACHA6B_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950197.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2674..2988)
                     /locus_tag="ACHA6B_002668"
     CDS             complement(2674..2988)
                     /locus_tag="ACHA6B_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950198.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDNTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3095..3310
                     /locus_tag="ACHA6B_002669"
     CDS             3095..3310
                     /locus_tag="ACHA6B_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950199.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3610..3852)
                     /locus_tag="ACHA6B_002670"
     CDS             complement(3610..3852)
                     /locus_tag="ACHA6B_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950200.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3839..4267)
                     /locus_tag="ACHA6B_002671"
     CDS             complement(3839..4267)
                     /locus_tag="ACHA6B_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8950201.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1371 a          832 c          653 g         1485 t
ORIGIN      
        1 ctcttttttt acgattattg aaaaaaaaag ataaaacttg attaattgaa gtattgctta
       61 aatttggaag ctatgaggga agaataaaag agttaggacg ggattatcta ctatttttct
      121 ttgttttaaa tatttgttat ctttgaacat aaaagagaaa tgtgtcgact gcagtgttgt
      181 gtccctcatc acgaataaac ggtgttcaac tagctgatct tgtattcact taaaaaatgg
      241 taaagtacga gttgctattc cccattttca attttctaac gctcaggaca aaatactgtt
      301 ttcacaacct cattggaata aaagcatata tattgtttca agaacgaaat cttgttatct
      361 ttttaaaggg catctgtcca aaatctaatt ttaggaggta cgttttggta tttattcgta
      421 caaataaaag agaattatct ttattcatgt aacgcatcca attgattttt tcatgtacgc
      481 tcctggaacg attttactga cgattgctta tttaactgcg aatcgctcat tagcttttat
      541 catagctatc catattttga ataacgttct tggctttgta ttatagatac agatgacaaa
      601 aaaagttcaa gtatgcggtg tagaaccaat atcgcatact tgaacttttt tcaatgagta
      661 taaattaaaa cttctaattt tcaattccca tcatctgata tgcccgaccg tctgaacttc
      721 ttttgaaaaa gccaaatgtg attagataac gtctttgttc gacataatcg ccactagaaa
      781 atacgttcag cagtatcgaa ttaacctctg tttctgtata agtcactcca tggtcaaagc
      841 gtttcaagac ttctttcaat aggatgtatt tcttttttgt tgaacttggt acttgtttta
      901 cttttccatc attcatgaat gtgtctaaaa ctttcatctt ttcttgctcc gtataagtat
      961 aaaccatctt ctttctcctt ttgattctaa gaatactcca tttatgttag aatattctgg
     1021 attttttatt aaagcggtta ctcttaagaa agatattatc atagtgctct tttttttact
     1081 actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa ttaaagaaaa
     1141 aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa taccaagggt
     1201 tctacccttg aataccaagg gttctttctc agtctgataa caatgattaa cgacggattt
     1261 ctttgatact tgtgaaaagt cgttaaagta ttgatataaa aggattttta ttacctctga
     1321 ctacatattg actaatttgt taaaaaatcg tttttcaaga atcctgtaat accagtgctt
     1381 tcaccgtttt tttaactctc taaagaacta aaagcaatca gaggttttac cttttcattt
     1441 gacgttaagt ggcgataaat tttcgaagtt gtttggtcag tatggcctaa gcgatctttc
     1501 aagacttccc agctacagtg ttccgctact aagcttgcca ttgtatgacg agctaaatga
     1561 gtatgaaatg gcttattaat tcctgagcga ttcacgcacc gtttgacgtg atcatttaca
     1621 tactctcgac gataaggaac accacgttta ctagaatcaa gatacacaaa caaatatgtt
     1681 tcttctataa taaacctgta atgttgcctc atctcaccca aagccttaat caactgttgt
     1741 agcttttcca taactagagg agttactaga actcgtctag cagaactaac tgtcttagta
     1801 gtttcaattc gaaaatcctt actcttacct ttaactgccg tttgcttata gaacagaatt
     1861 tcatttcttt tgaaatctat atctccaggc tgtagagcaa gcgcctcgct gactcggcag
     1921 ccagtgtaaa atagaacatg gaaaagtaca gacatcggta attcctcatg aacaatgcca
     1981 taattcaaaa aagttctcaa ttcatcgaca gtaagaaact tgtcctcaat attatttatg
     2041 gcttctttta aatcagttac cgttaactga tattttggca ttctcacaga cttcattgga
     2101 ttttcactta tcaaattctt tctttgacaa tatttaaaaa tcatatttag aacttgaata
     2161 agtgattgta aactattttt ggcatagcca acatttgttc cattaacgcc acattccttt
     2221 aaggtaaaaa gcaattcttg aaggcactct ggtgtcaaat cagacaactt tttatcacca
     2281 atccatcgaa caataatttt taattgttcc tctcgcttat cataggttat ttccttttgt
     2341 tcatttaacg aacggtaata ttggaaccat tgctcaccta caacactaaa cttgtcattg
     2401 cttgcaacaa tttttttctt aagcctttgt tgtttctcca tttccagata agcgagttgc
     2461 gcctcacgtt ttgaattaaa gccacgtctt gttgttcgat atgcttgaaa gtaccatgtg
     2521 atcctaccag tagaactgat ttgcttcttg aatcgatctg ttttttccat ttattgattc
     2581 ctcctatttt gaaggaaccc aacaagcaga aaccctttgg aaatctttct atctatgtca
     2641 tcattgtacc gcatcttaga aagaaaatgt aacttataaa gatcgaagta gtcgcaattt
     2701 ttctcgatag tcattcgaat cactctttct catccattct aaaacatcat cacgaaaata
     2761 gtgtcctcga atcccgtacc aacagggagg aaactcacca agttttacta aacggttcag
     2821 tgtattatca cttatctgaa atagtcgctt taaatcagta tttgttaata ccagaggtaa
     2881 atgactgact gcaagcaatt tttccgtgta atcttccagt tttttttgta aaattgaata
     2941 gaactctatt tccagagaat taacatttgt tttaacttcc aactccatca cccttctctc
     3001 tcaaaatcaa aacgaccacc acggtacccc ttaaaattgt aaaaaaacac tccagtactc
     3061 catcaaaacg gactagaaaa gaggaatttt taatatgaaa caaggaattg cagacataaa
     3121 aataattaaa gaaattcttg aaaaatctac ggctaatgca atagcattcg gaacaggaat
     3181 caacttgagt acagtaaaaa aacttaaaag tggagagaga gctgaagaaa aattaaatct
     3241 tgccgatgct attaaaatca cagaatttgg catgaaaaat atgccaacca aaatcgaaat
     3301 atggaaatag tagtttttct gatctatcag ctgttttgcc aaagcacata ggaatctcac
     3361 ctccccccag taatggcggg ccgatttggt tacggaagta tcattatcgt agtcgcgatt
     3421 ctcatattaa aatttgtcgc ttaaaagcta accttgtcat ggcgtaacta gagaatcaaa
     3481 atctacatgt actgatagat ttgctattca tttgtcaaag agcgctaggc tattttcagc
     3541 ctatggaaat cctttatttt gacccgataa aaaacttcca tagaatgaaa aggaaaggga
     3601 aaaaatactt taacgagttg tatcaaattc aagtatcttc tcaatcaatt ttgtttccag
     3661 ttgtctacgc atatattcat ctacatacat aaccattcct ttttcttctt cataaactgg
     3721 tttcatggct aaccgtgaaa tataattttg atattttgac agaacgatgg acatggcaat
     3781 tgaatcacca gaagctgcgg ctttgattgt cgttagtggt atgaatggta tatatcgttt
     3841 attttgcttc atatttgaca cccaaatact ttcctagctc ttccaaagcc atttgtcgtt
     3901 ggtaccaaac actgccgaga cttttcccaa taatatcgcc aatttcacga tcattaagac
     3961 cgatgaagaa ggacataaaa atgatttctt gtctttgact aggtaatgct gcaatcgcat
     4021 ttgccaactc ctcattggaa ataaagattt tcataccaag gacaataaaa gtaccactac
     4081 tattcaaatt tggttccaaa taatagaact ctgtacctaa ccctttttgt tccaatattt
     4141 ccattgatat ttgccgttgg cgaaatctag cgtacttctt ttgaatattt aacgcctcat
     4201 tgcagactac tttcttacaa aaactgtcaa acgtatgttg aatgtgggtt tcctctgagt
     4261 cgcccataat attctccccc tcaaaaaatt cattcaactc gttttacttc tatccctttc
     4321 cattattact acaatctagg a
//
LOCUS       DAYYHB010000153         4041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000153 DAYYHB010000000
VERSION     DAYYHB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.158"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..775
                     /locus_tag="ACHA6B_002672"
     CDS             179..775
                     /locus_tag="ACHA6B_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8950202.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ACHA6B_002673"
     CDS             899..2698
                     /locus_tag="ACHA6B_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8950203.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ACHA6B_002674"
     CDS             complement(2976..3188)
                     /locus_tag="ACHA6B_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950204.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="ACHA6B_002675"
     CDS             3650..3799
                     /locus_tag="ACHA6B_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950205.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DAYYHB010000154         4030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000154 DAYYHB010000000
VERSION     DAYYHB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.159"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..703
                     /locus_tag="ACHA6B_002676"
     CDS             170..703
                     /locus_tag="ACHA6B_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950206.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="ACHA6B_002677"
     CDS             715..918
                     /locus_tag="ACHA6B_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950207.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1241..1621
                     /locus_tag="ACHA6B_002678"
     CDS             1241..1621
                     /locus_tag="ACHA6B_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8950208.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1603..2541
                     /locus_tag="ACHA6B_002679"
     CDS             1603..2541
                     /locus_tag="ACHA6B_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8950209.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDHVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNPKTRTYDQFCDSLKIFGIHCFNRGKNFTYELIKKKK
                     KVRSNKLGKDYEKETILHELDRREQTYYRERERAYHRQQADELRRARESIRKIAGEVR
                     REQRTFTPEQSEIRSTRRNHEQHVERGTDHQQELSL"
     gene            2501..2779
                     /locus_tag="ACHA6B_002680"
     CDS             2501..2779
                     /locus_tag="ACHA6B_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950210.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTIDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLFAFWLVWGG"
     gene            2781..3470
                     /locus_tag="ACHA6B_002681"
     CDS             2781..3470
                     /locus_tag="ACHA6B_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950211.1"
                     /translation="MAFYLPYLLIFVSISGSIWLSCKIYQTRHSLKGSKIRFKRFLLL
                     GCIFSLIIISSGLFGILEGNKRISGSILLENVTQKYEVARDKKKKEQALAQKIEEFTA
                     CYENMNDIFVKQEKRLTDKNMETLTRLYRKLPEKQQKEYQEKYEQVKKDVQYVKDTQT
                     EEACYDLFSDTIPFSTSEQERKERQQSVTYERYEILLQKATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
BASE COUNT         1454 a          698 c          800 g         1078 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtataa tgagtattgc
     1201 gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa
     1261 tttggctagc gatccttcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt
     1321 tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag
     1381 tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc
     1441 gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca
     1501 aattgctaag tggtgcaaca cacatcaaca tgacgttgct cctggagacg cccaacgctt
     1561 atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta
     1621 agtcgaacca cttcatgtag ccgagcaatc aattacgcag aaccacgagc aaccgtcaaa
     1681 acaggaataa actgtgacat tgattatgcc aaaagtgaaa tgaaacaaat acgtatgctt
     1741 tacggaaaag acgatcatgt acaagcacac ttattgattc agtcctttcg accaggagaa
     1801 attaccgccg aaaaagccaa ccagctagga aaagaatacg ctgaaaaaat cgcaccagaa
     1861 caccaaatcg cgatttatac gcacacggac aaagaccata tccacaatca cattgtcatt
     1921 aattcggtca atttagaaac aggaaaaaag tttcaagcac atggtcaagc attcctagat
     1981 aagtgttacg atatcaacga cgaaatttgt ttgactcatg gtttaagtat cactgaacga
     2041 ggaaaaaaag aagaaaaacg aactatgtct gaaatcaaac taaaagaaaa aaatgaacct
     2101 gtttggaaag acgaaattcg ctttgccatt gatcaaacca tgaaaaatcc aaaaaccaga
     2161 acctatgacc aattttgtga ttccctcaaa atatttggga ttcattgctt caatcgtggg
     2221 aaaaatttta cttacgaatt aatcaaaaaa aagaaaaaag tacgatccaa caaattagga
     2281 aaggactatg aaaaggagac aattttacat gagctggaca gacgagaaca aacctattat
     2341 cgagaacgag aacgagccta tcatagacag caagccgacg agcttcgaag agctcgagaa
     2401 agcattcgga aaattgctgg agaagtacga agagaacaac gaacttttac gcctgaacaa
     2461 tcggaaatta gatcgactcg aagaaaccat gaacaacacg ttgaacgagg aacagaccac
     2521 caacaagaat tatcgctcta aaacgttaaa agacatagaa aacgaaataa aaagacaacg
     2581 tgcttttctt gcgccttata cccaagaaag gatacaaact acaattgatt cacaagtcaa
     2641 aaaactccac caagcgctat tagaccagcg aaaccaagaa caaaaaaagg ctttaattca
     2701 acgatcgatg gatattcttg cccccttatg ttgcttagtc ctgacactgt tcgcattttg
     2761 gttagtatgg ggaggctaaa atggcatttt atttacccta tctactcatt tttgttagta
     2821 tttcaggaag tatttggctc agttgtaaaa tttatcaaac aagacattca ttgaaaggct
     2881 ctaaaattcg atttaagcga tttttattgc ttggctgtat attttcattg ataatcattt
     2941 caagtggctt attcggcatt ttagagggaa ataagcgcat ttcaggaagt attttattag
     3001 aaaatgtcac tcaaaagtat gaagtagcac gtgataaaaa gaaaaaagag caagcattag
     3061 cacaaaaaat tgaagaattt accgcttgtt atgaaaacat gaatgatatt tttgtgaagc
     3121 aagaaaagcg attgacggat aaaaatatgg aaacactcac tcgtctttat cggaaattgc
     3181 cagaaaaaca gcaaaaagag taccaagaaa aatatgaaca agtaaaaaaa gacgtgcaat
     3241 atgtgaaaga tacgcaaaca gaagaagcgt gttatgactt attcagtgat acaatccctt
     3301 tttcaactag cgaacaagaa cgaaaagaac gacaacaatc agtcacatat gaacgctatg
     3361 aaatcttgct tcaaaaggca actaacatac aaaatccaac gaaaaaagaa acagcattaa
     3421 attatctaga aagtgtcaaa gaatggctgg atcagcaaca gcaaaattga atttgattga
     3481 aactcatgtt aaactaaata tgcaaatagg taatcataag atacacgaaa gtcctagaga
     3541 agtcggattc tctaggactt tttttgtagg ctgatatcgc cttagttgat tttacttctt
     3601 gtttcggtta ttcagccaat aactaaataa agagtttaga agctcaacaa gaagggcgca
     3661 aataaagtgg gtaatcataa gatacacctc cttccagtcg caagactgtc ggataggcga
     3721 caagttatta tatcttaaaa aattaaatct gtgtggtgtt tttaagtatc ccttatttta
     3781 gggttgttag ggcttgccct gaccgcctgt aaggcgcttt aggaactgat aaggttttcc
     3841 cctagaactg tctcaaaacg gtttagacga gggctcagag ccccacaagg ttctctttga
     3901 acaacggttt tggcaaagga gcctttaggc gactgcgcag tacaatgtga gagcatagcg
     3961 atcacacctt tttagcgacc gtagggagca agttttccag tttagaaaaa ttcgcgaacc
     4021 tgtttgagta
//
LOCUS       DAYYHB010000155         3986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000155 DAYYHB010000000
VERSION     DAYYHB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.160"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA6B_002682"
     CDS             complement(<1..132)
                     /locus_tag="ACHA6B_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8950212.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            complement(483..1442)
                     /locus_tag="ACHA6B_002683"
     CDS             complement(483..1442)
                     /locus_tag="ACHA6B_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950213.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1557..2039
                     /locus_tag="ACHA6B_002684"
     CDS             1557..2039
                     /locus_tag="ACHA6B_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8950214.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            2076..3086
                     /locus_tag="ACHA6B_002685"
     CDS             2076..3086
                     /locus_tag="ACHA6B_002685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8950215.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3201..3914)
                     /locus_tag="ACHA6B_002686"
     CDS             complement(3201..3914)
                     /locus_tag="ACHA6B_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950216.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1285 a          692 c          780 g         1229 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttcgtt
      481 tgtcaaatta agttagacaa atcatctttt gtgacgtaac tcaaaaatac ttccattggt
      541 gtccgatagt gtaatgattt tctaggaata tgatttcgtt tcgatgccac cgattggata
      601 aaggtttcag atacaccatt aaaatccata gctttataca aaccatcttt acgtaataag
      661 ccatttgaat gttcattcaa tcctcgttga gaaggtgttc ctggatccgc aaaataaatt
      721 gaaatatcgt tggtattact aatctttttc caatttgaga attcttttcc acaatcaaat
      781 gtgatggatt taaataagtt ttttgggaca caacgaaacc attgattcag cctattttca
      841 atgtctttag cctgtctgcc ttcgggtttc aaggtaatga tgactttaga tagccgttca
      901 actaatgtga tgaccgcgct tttatgtttt ccacccacga tagtgtcacc ttccaggtga
      961 ccaaattcat gatcaaactg ggtatagtct gtctttctgt ctcggattga ccggcgaaac
     1021 ccttgttttc ctcttttttc tttatgaccg tttggttttc tttttccctt cattggtaaa
     1081 tcgtgagtgt caaaggtttt atctttaaac attcgataca gtgtgcgtac cgaacaagca
     1141 ataggaaact ctgctcgacc aataaggaca tcaggagccc acccttgaac aacctttttt
     1201 tgaatgtatt cctgttgctc atcaggtaaa acaatcggat gtcttccgca gttacgtttg
     1261 tttttcttat attgtttata gtagctcatt gcgcttccac cagatttaag aaaacgatag
     1321 accttataaa tagtttgtcg agaacgttgt aacagattgg ctacttttga aacaggttgt
     1381 gattgattga aataagcttc tatcattacg agttcagttg gtgtaagatg gttataggtc
     1441 atttgtactc actccttata attctttcgt cggaactatt ttgagtgtag cacaaatgac
     1501 tttttttagt tgtctagctt aattttacaa tcggcgaata gaaaagagac ataaaaatga
     1561 ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat catcatcgag
     1621 ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt acaggtacag
     1681 agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat ctgactttta
     1741 ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg ctttctattg
     1801 tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg
     1861 gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat gaaaaacttg
     1921 tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac ttaaacaaaa
     1981 tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac
     2041 caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa
     2101 gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt
     2161 tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga
     2221 cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca
     2281 atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gccttgggga ggagaattat
     2341 tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt
     2401 attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac
     2461 atggaaccaa ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg
     2521 aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaaccttct gaaaagtatc
     2581 aaaaagagtg gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa
     2641 attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggctgta
     2701 ttgatacgat cagtcattta atcggtaccc cttatggaga tgtccaatct tttaaaagaa
     2761 agtataatga tggaaagccg atcttgtggt ttctagaaaa ttgtgagcta tcgtcagttg
     2821 atttaagaag atcacttgtt cagatgaaac tggcaggatg gtttgatggc agctcgggga
     2881 tcatgttcgg tagaagtgca gcagaggatt tgttagatta taaaatgaag gatgtatatg
     2941 aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac
     3001 cgccacaact gacattgatc aatggtgcct atgcagaagt agcggtagaa gatagaaaag
     3061 cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtt tatcaaagac
     3121 caacaaatct aaatagaaaa gaaaatgaaa gaagctgaca gtattttgcc agcttctttt
     3181 tgtatgatct attagaaatt ttaattatcc ataactatca cattgatcag ctgtttcaca
     3241 tcatttggat tacctgtagg aatagctact acagatgcat tatcaataat ttcttgagtt
     3301 gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca atgtgaagga
     3361 actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac tccctggtaa
     3421 ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc tgcatcataa
     3481 ggggcgatag gtgttcctgc aggataatga ataacatgta gttcgaaatc acccgatgta
     3541 tctctaaaag tattacctct aattgctagc caaacacgtg cgtactcaat ttgtagatca
     3601 gaatatcctg ctaacggatc tattgactga gaagactgtg aggaagttgt gtctattgtt
     3661 gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt gggctctgag
     3721 actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg agtacttgat
     3781 ttttcagctg aactggtttg tttgattgaa cttttgtctg tggtcgcaga tggatcacta
     3841 ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa taaaataaaa
     3901 cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag cataatcctg
     3961 aatttttgaa tccgtaaaac cttgtc
//
LOCUS       DAYYHB010000156         3866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000156 DAYYHB010000000
VERSION     DAYYHB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.161"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..469)
                     /locus_tag="ACHA6B_002687"
     CDS             complement(248..469)
                     /locus_tag="ACHA6B_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950217.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACHA6B_002688"
     CDS             complement(567..812)
                     /locus_tag="ACHA6B_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950218.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACHA6B_002689"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACHA6B_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="ACHA6B_002690"
     CDS             complement(2036..3037)
                     /locus_tag="ACHA6B_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8950219.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="ACHA6B_002691"
     CDS             complement(3196..3462)
                     /locus_tag="ACHA6B_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950220.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="ACHA6B_002692"
     CDS             complement(3452..3808)
                     /locus_tag="ACHA6B_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8950221.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1434 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaatt
//
LOCUS       DAYYHB010000157         3860 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000157 DAYYHB010000000
VERSION     DAYYHB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.162"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..546)
                     /locus_tag="ACHA6B_002693"
     CDS             complement(<1..546)
                     /locus_tag="ACHA6B_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8950222.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKF"
     gene            complement(662..1228)
                     /locus_tag="ACHA6B_002694"
     CDS             complement(662..1228)
                     /locus_tag="ACHA6B_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFM8950223.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1364..2017)
                     /locus_tag="ACHA6B_002695"
     CDS             complement(1364..2017)
                     /locus_tag="ACHA6B_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950224.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(2067..2495)
                     /locus_tag="ACHA6B_002696"
     CDS             complement(2067..2495)
                     /locus_tag="ACHA6B_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8950225.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2500..2874)
                     /locus_tag="ACHA6B_002697"
     CDS             complement(2500..2874)
                     /locus_tag="ACHA6B_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950226.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            3178..3363
                     /locus_tag="ACHA6B_002698"
     CDS             3178..3363
                     /locus_tag="ACHA6B_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950227.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3376..3624
                     /locus_tag="ACHA6B_002699"
     CDS             3376..3624
                     /locus_tag="ACHA6B_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950228.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3655..3825
                     /locus_tag="ACHA6B_002700"
     CDS             3655..3825
                     /locus_tag="ACHA6B_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950229.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         1126 a          682 c          653 g         1399 t
ORIGIN      
        1 aaatttatct ttaattttta tcgtttttga ctctctttta taatcaataa ctacttcatc
       61 gataggattt ttttctacat aacctttttt catagcatat gaaaaaagaa gattcaattt
      121 ggatttaatt acactgacgt acttatttga caagtcatct ttatatatca tgtcttcaaa
      181 aatattattc aaatctattg tattaatacc agaaacgatg taagtctcag gtatcttctt
      241 ctttatagtg tttaatatat tattcgtagg ataatatgta gattccttaa cttgtctttt
      301 gtatataact aaccattctt caacaagctc atgaaatgtt agatcaggct tatgaagtgt
      361 tttttcgttt aattttatat caattttttt atttaactct agcaatgcca ctttctgagt
      421 ttctcgagat ttatttttat aagtaatgct cacttttttt ctttttctag tttttggatc
      481 aatatatcgc tcattatatt tatatacttt ttttccgttt ttatcagttt tagtttcgat
      541 ccacattgtt ttcatctcct atttgttgct ataataggaa tagataagta agccgattat
      601 agcaggttta tttttcatca cgtccacaaa ctttggcgag ggaggggcgt gtttttgtta
      661 gttattgtgg taggttatct aaagcgtatt gtgcttgctc atttgtaaaa ccttcaaata
      721 taagttgatc gtacaattgc gaatctgaca ttgatgccca atcatcgtaa ctttccgctt
      781 tagccaaagc ttgttcgttg taatcgacat caacattagc cagcgcataa tcgattgcat
      841 cacttggata ttcttcaaac tctagttgtt cacgcaaacc ttgttcagac ataccagccc
      901 aacctagata gctttcagct ttgctcaatg catttctgta ttcacgagga acactttctt
      961 cttgagtaac ttcttctata gtagtttcag tagtttcagt ttcttcttca acgactgtag
     1021 attctgctac cgattctgca gaagacactt ctgtcacgct ttctttagtt gtttcagcga
     1081 cggtattttt agcatcatca ctgcctcctt gcgaacctat agcaataaac accacaacaa
     1141 ccgctaacaa ccaaaaccat acccgtttat aaaacggttt cttaacttta tacattttcc
     1201 catcttgacc cataactttt tttgccattt aaatatccct cgcttcttgt tataatatat
     1261 ttgtgatctc agaaatgagg tatgagtccg tgttgcagca cgggcttttt tctttataac
     1321 ttttttagag attataggca aaatagtagg gcataaaaat gtattattga attccgtatt
     1381 tagaaaatcc taattgaact tcaccggaag tcttttgctg tgttgtacgc aatgcttctt
     1441 cttcagacat tccattctgt actttccatg caacaggcga catcccgtat ttgttaacaa
     1501 aatcagtaag tgataaagtg tcagcgtctt gctgagcgct tgtttgttgg tcttctggat
     1561 tctgttgaga tgctgcttgt tgttctttct gatcttgact gataatattg ccagcatcat
     1621 ctgtagtcaa tccattttca taaagggcca cgccgaaagc ttcccactct ttgttggacc
     1681 aatttgcacg atcagctgga gttgactgta aagtgcgttg tttcatctgt tcatatgttt
     1741 cttcttgagg tgcggtttgg attgtactct gactggagct tataactgtt gggctaggtt
     1801 ccgccgtagc ttggttggag cttgtaactg ttgaactaga atctgtttta gatgtagatt
     1861 tgctagtaga tgaactggtt tcagttgttt cttttgtttt acttattttt gtttcttggt
     1921 tagaagtggt atctgttgat tcagcttttt tattatttga acaagctgaa agtagcagag
     1981 cagtacttaa caacaacata acgctaactt ttttcatttt ataattcctc tttctcgttg
     2041 taatatgtgt gctaacacgg gcttttttat ataagaaaac gataagcgct ttctggaagc
     2101 ccgtaaagat tctttaattc ctcgattttt ttaggatatt gatcattatc ttctttataa
     2161 agagaaacaa tgagattagc agcaaagcaa ttagcttcgc tttcagattt gcttctagat
     2221 gttcttgttg atacataata gctggataag ccacgatgaa aaatagcgtg acctaattcg
     2281 tgagcgcaaa tgtagaatct ttcctcagag tctcgcagtt catcatttaa aaagattatc
     2341 gcacgatctc taatttcttg aaactgcccc ttgggattct cgataaaagg aacgtattga
     2401 attttaatgc ccatcttttc acaaatataa aaaggattag cggactggta tttccgcttc
     2461 aactcctcga ctaaattaat cgtatccatc tccataagct cacatctttt tgcctttttc
     2521 tttgtcttct ttcacaatat cccagaaagt cgctatcagg atatctttta cgcgctgtat
     2581 ttgttcgggt gtcaatgttt ccccaccata agacatatta acatttgagt ctagtagttt
     2641 atcaagttca accacttcct cttttgtagc ccacttggga acattattat ttcccaataa
     2701 ataatcagtt gtgacaccaa aataatcagc aactttcttt aagttctcag attttggcga
     2761 ggctttatcc catcttctta tttgtccatt agagatgccc acctgtcttt ctacttctgc
     2821 tatagtcaca tgcttttcgt ctgctaattc tttaatctta gtaactaaac tcattattat
     2881 caacctttca aagctgaaaa gaaaataaat agcttaaaag ttatattttt ggttgacaat
     2941 tagcttttaa gctagtatat ttaattcgta agctaaattg ttagctaaat aagagcaaca
     3001 aaaaactcta ctaatttaaa acattctctc ggtcgccaaa cttagaaatg ttattttaga
     3061 ggctttttat aagtcttatt taactatgta ttcataatag cttaaaagct aataggtgtc
     3121 aacgatttag ctaatttttt agcttacaaa ttatttgttt agaaaggagc tatttttatg
     3181 tctgagaatt tagacttaaa aattcgagcg gagatgagaa aaagaagaat gactttcaaa
     3241 gaactagctg cgcttatcgg tatttcagga gcttatttat cagatattct aaacggcaat
     3301 cgtgatggaa agaaagcaca acagcatatc gaaacagtga aaaaaatatt ggacatccga
     3361 tagggggggt aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa atataattat
     3421 tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg
     3481 gcgtatgaac gggtcacagg acaagaagtt tggatcaata cagaattata cgataaattt
     3541 ttgtctttca aatcctataa caggttacgc acaagaaagg taacacctaa agaatttatc
     3601 gagaagcatt tagttgattt ataaaaaaca gaacattttg agaggtgaag gttaatgggt
     3661 aaattcaaca gagcattagt attcagcgca ccgctaatcg tctatgcttt agggctttgg
     3721 ggaagtagac aagcgttgat aggaacgatt gtgtacatgg tttggatttt tatagggctg
     3781 gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat gagtaaggac
     3841 gatattgtat ttctgttaat
//
LOCUS       DAYYHB010000158         3757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000158 DAYYHB010000000
VERSION     DAYYHB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.163"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..552)
                     /locus_tag="ACHA6B_002701"
     CDS             complement(154..552)
                     /locus_tag="ACHA6B_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8950230.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1294)
                     /locus_tag="ACHA6B_002702"
                     /pseudo
     CDS             complement(549..1294)
                     /locus_tag="ACHA6B_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            complement(1291..1974)
                     /locus_tag="ACHA6B_002703"
     CDS             complement(1291..1974)
                     /locus_tag="ACHA6B_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8950231.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2141..3028)
                     /locus_tag="ACHA6B_002704"
     CDS             complement(2141..3028)
                     /locus_tag="ACHA6B_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8950232.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3025..3621)
                     /locus_tag="ACHA6B_002705"
                     /pseudo
     CDS             complement(3025..3621)
                     /locus_tag="ACHA6B_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(3630..>3757)
                     /locus_tag="ACHA6B_002706"
     CDS             complement(3630..>3757)
                     /locus_tag="ACHA6B_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950233.1"
                     /translation="QTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1223 a          815 c          624 g         1095 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattaaa tgctgtccta
     1081 ccggtacata agccatattg actccgtgca ggaaaaggat caacccaata gtcgttatca
     1141 agaaaccttt cataatgtca aagaaacgct ttttgcgcat acgaaaacta atgatattca
     1201 ttaaaacaaa taggattacg ataggtgcca ctgccaaaat gacttccatg gtaacgattt
     1261 cgattccatc tatcacttgc tgccagaaac tcattggaag atcaccccca aaatcatcac
     1321 ggcaagaatc ggccctaacg aagcaatacc gaccattcca aaactgtcat tttcattttt
     1381 cttttgtttg atcatactag tcatccctcc ggcaagggcc aagataaatg gaacagttac
     1441 tggaccagta gtcactcccc ccgaatcaaa agcaatcggc ataaattcgg aactggtgaa
     1501 aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc aattgataat aggatagctt
     1561 gaatacgacc cgaagcagcg caaaggctag aaatactcca gtgccgacgc tgacgatccc
     1621 aatcagcaac actctgccaa tctctccgtc agatatatca tatacttgtt gtcccaaaac
     1681 ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata gcaaatccca aactgaccaa
     1741 aacggtcaaa cttttctttt tgatcatata tttccctact agatctccga cttccatcat
     1801 agaataatct gctccaaaca aaaagagcgt cattccgatc atcatgattg ccgcaccaac
     1861 cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa acaaacgtta aaatgacaat
     1921 caggacagtc atcggcaata tagcagcaat cacttcttta aaatttttta gcatgtatcc
     1981 tcctccttta ctctttaagt ttaccttaaa atgaaagagt tttcgagcac aaacttaaaa
     2041 cgttatcatt taatttttct caacataaaa taacacacgt cccaaaagta tctttttact
     2101 tggaaaaatg actttgaagg aacgcgctta ttgtgttttt ttattttaca gagccgatca
     2161 ttccttgaac gaattgcttt tgcaaaacaa aaaatacgat ggcagttggt aaagtagcga
     2221 tgactaatcc agccatgatc attccatagt ctggtgtata ggaagcaccc atagcagata
     2281 aaacaagcgg aaccgtccgt ttatcaggag attgcaaagc tactaagggc cataaataat
     2341 tgttccagct actcatgaat gtgatgattg cagccgcggc ataggtattt ttagccgtcg
     2401 gcatatacac tctaaaaaaa atacttagtt cttttagacc gtctaatcga gcggcttcta
     2461 ctagatcttt tggaaaagat ttaacatttt gtcgaaagaa gaagatcaaa aaagcagtgc
     2521 taacagatgg caggatgaca accaagaaac tgttgattcc caaaaccgtt ccgttcaatt
     2581 gtgaaaacat tcgatataaa gggatcatca atgccgcaaa aggaaccatc atcgatagca
     2641 gtagaatatt gaaaattagg tcttttcgtt tactttgata aatctcaaat ccatatcctg
     2701 ccattgacga aatcagcaat gcaaacacag tggtcaccaa agcgatcatc gctgaattcc
     2761 aaagggaacg agtaaaattc agctcattgg aaaacaaatt ttgaaaattg atgatcaagt
     2821 tactgccaat ctttattttt cccgatgtga tatccacggg tgtattcgtc attcccaaaa
     2881 ccatccagac aaaagggaaa atggaaataa atgataggat gcttaatgct gtatacttta
     2941 atactgaccc aattgtcgaa cgaagttcta atgcttgttt tttgacgttg gggcgttcga
     3001 ttgtttcttt ttctaccata tttttcatcg actttctcct cctactttcg tttggattat
     3061 cgacaatatc acaataaagg aaacaataac aaaggaaact gctgccgcat atccaaaatt
     3121 aggcgtgaat ttataactca aattatagat gtattgagat agggtggtcg ttgcatttgc
     3181 tggtcccccg ccagtaatat tttgtacttc atcaaaaagc tgcaatgtcc cgatcgtcga
     3241 agtaatggaa gtaaacaaaa tgattggttt taaatttggg atcgtcactt tgataaactg
     3301 ctgccattta gaagcaccgt caatagatgc tgcttcataa atctccgaat caatattttg
     3361 catgcctgaa aggaaaaaaa tcatattgta tccagtccaa cgccaagtga tcgaaagaat
     3421 gatcagaact ctcgcccaaa ttggatgagt caaccagcca atcggtgtgt caatcatacc
     3481 aatgcccaac aacaagttgt ttacaaggcc agattgagaa aatagacttt tcatgatcag
     3541 agaataactg accatcgaag tgatacatgg aagaaagata gcggttcgaa acaacccttt
     3601 atatttcaac tttttatcat tatgatttgt caaattaagc tagaataaaa tgcttcttgt
     3661 acatagctca aaaatatttc gattggtgtt ctgtaattca atgattttct tggaatatga
     3721 ttacgttgat tgctgacact ggaaataaat gtctgat
//
LOCUS       DAYYHB010000159         3680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000159 DAYYHB010000000
VERSION     DAYYHB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.164"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHA6B_002707"
     CDS             <1..276
                     /locus_tag="ACHA6B_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFM8950234.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACHA6B_002708"
     CDS             419..640
                     /locus_tag="ACHA6B_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950235.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACHA6B_002709"
     CDS             656..1498
                     /locus_tag="ACHA6B_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950236.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACHA6B_002710"
     CDS             1561..3363
                     /locus_tag="ACHA6B_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFM8950237.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3680)
                     /locus_tag="ACHA6B_002711"
     CDS             complement(3422..>3680)
                     /locus_tag="ACHA6B_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8950238.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          522 c          750 g         1097 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaatggatat agtaatgccc
//
LOCUS       DAYYHB010000160         3618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000160 DAYYHB010000000
VERSION     DAYYHB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.165"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..296)
                     /locus_tag="ACHA6B_002712"
                     /pseudo
     CDS             complement(1..296)
                     /locus_tag="ACHA6B_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(556..1104)
                     /locus_tag="ACHA6B_002713"
     CDS             complement(556..1104)
                     /locus_tag="ACHA6B_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950239.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1105..1962)
                     /locus_tag="ACHA6B_002714"
     CDS             complement(1105..1962)
                     /locus_tag="ACHA6B_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8950240.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2248..2534)
                     /locus_tag="ACHA6B_002715"
                     /pseudo
     CDS             complement(2248..2534)
                     /locus_tag="ACHA6B_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2524..2853)
                     /locus_tag="ACHA6B_002716"
     CDS             complement(2524..2853)
                     /locus_tag="ACHA6B_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8950241.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2930..3202)
                     /locus_tag="ACHA6B_002717"
     CDS             complement(2930..3202)
                     /locus_tag="ACHA6B_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8950242.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3202..3465)
                     /locus_tag="ACHA6B_002718"
     CDS             complement(3202..3465)
                     /locus_tag="ACHA6B_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8950243.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1053 a          733 c          465 g         1367 t
ORIGIN      
        1 ttgatctcgc ttattacaat cgctttcatt tgtcctaaga gtcaaatctt tttccaccat
       61 aacttttaca cccaataaat tttgcggttt atttttctct gttgaaattc tttgtgtagc
      121 ctttaaactg acaattagct cattgggtac atacttattt gagagtcatc taattggctc
      181 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      241 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcacctac
      301 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      361 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      421 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      481 cctctatttc atgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      541 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      601 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      661 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      721 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      781 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      841 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      901 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
      961 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1021 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1081 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1141 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1201 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1261 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1321 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1381 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1441 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1501 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1561 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1621 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1681 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1741 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1801 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1861 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1921 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     1981 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2041 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2101 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2161 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2221 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2281 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2341 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2401 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2461 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2521 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2581 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2641 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2701 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2761 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2821 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2881 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     2941 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3001 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3061 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3121 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3181 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3241 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3301 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3361 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3421 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3481 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3541 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3601 gttaattttt tcctagag
//
LOCUS       DAYYHB010000161         3571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000161 DAYYHB010000000
VERSION     DAYYHB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.166"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..952
                     /gene="racE"
                     /locus_tag="ACHA6B_002719"
     CDS             131..952
                     /gene="racE"
                     /locus_tag="ACHA6B_002719"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8950244.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            955..2310
                     /gene="rph"
                     /locus_tag="ACHA6B_002720"
     CDS             955..2310
                     /gene="rph"
                     /locus_tag="ACHA6B_002720"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8950245.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2311..2829
                     /locus_tag="ACHA6B_002721"
     CDS             2311..2829
                     /locus_tag="ACHA6B_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8950246.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2936..3436
                     /gene="cbpB"
                     /locus_tag="ACHA6B_002722"
     CDS             2936..3436
                     /gene="cbpB"
                     /locus_tag="ACHA6B_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8950247.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         1221 a          602 c          808 g          940 t
ORIGIN      
        1 aaagtcacaa ccgcttttcc tctttccatt cgaaaaaaag agatttcgat tgttttaagc
       61 aagtaagagc acttgaaaat tcacgctaaa aagtacacaa tagggaggat agaatttatt
      121 gaaaaaaagg atgatacgat tgacagataa tcgccctatc ggatttattg attcaggtgt
      181 cggcggcttg actgtagtaa aagaagccct gaaacaatta ccgaatgaaa atattttatt
      241 tgtaggagac acagcacgct gcccatatgg ccctagaccc gcggaacagg taatccagta
      301 tacttgggaa atgacggatt atctggtgga gcaaggaatc aagatgctgg tgatcgcctg
      361 caataccgca actgcggtgg ctttagaaga aatcaaagct gctctttcta ttccagtcat
      421 cggtgtgatc cttcccggta ctagagcggc agtaaaaaaa acacaaaata aacaagttgg
      481 cattatcggt acgattggta cggtaaaaag tcaagcttat gaaaaagcac tgaaagagaa
      541 agtaccagaa ttgactgtga caagtcttgc ttgtcccaaa tttgtttcag ttgtcgaaag
      601 taatgaatac cattcatcgg tggcgaaaaa aattgtggca gaaacattag ctcctttaac
      661 cactaaaaaa atcgatacat tgattttggg atgcacccat tatccattat tacgccccat
      721 cattcaaaat gtaatgggag aaaatgttca actgatcgat tctggagcag aaacagtagg
      781 tgaagtatct atgctgttag attatttcaa tttgagcaat tcaccgcaaa atggtcggac
      841 attatgccag ttttatacaa ctggctctgc caaacttttc gaggaaatag ctgaagactg
      901 gcttggaatc ggacacttaa atgtagaaca tatcgaattg ggaggaaaat aaggatgcgt
      961 catgacggga gaaaagccca agagcttcgc aaactggagc ttcaaacaaa tgttttcaaa
     1021 tatccagaag gatcggtagt gatccgtttt ggtgatacaa cagttatttg ttctgcaaca
     1081 atagaaaatt ctgttccccc ttttttgaga gaaacaggaa caggatgggt gacagcagaa
     1141 tatagtatgc tgcctcgagc aacgagtacg agaaatcgta gagaaagcag caaagggaaa
     1201 ctttctggta gaacaatgga aatccagcgt cttattggtc gttccttgcg tgcagtagta
     1261 gacttggaaa aattaggaga acgtagtatc atcgtagact gcgatgtgat ccaagcggat
     1321 ggcggtacta gaacagcaag tatcactggg gcatttgtgg cattgaaatt agccattgaa
     1381 aaacttttaa gagaaaaaga attgtcagaa gatccaataa aagaacatct tgctgcagtt
     1441 agcgtaggaa tcttaccaga tgggacttgc gtcacagatt tggattacca agaagattcc
     1501 gcagcacttg tagacatgaa tcttgtaatg acagaatctg gaaaattcgt ggagattcaa
     1561 ggaactggag aagaagcaac gtttgatggt gaacagttga atgaaatgct cttttttgga
     1621 aaaaacgcga ttgaagattt gatcaaagag caaaaacatg ctttgctaac agagtttgct
     1681 caaaatgatg agcggataga agaaacaaaa acaatcatca ttgcgacaag aaatccagga
     1741 aaagcagaag agtttcgaaa tatgttcaaa gaagcgggat atcatgtaaa aacattactt
     1801 gactatcctg aattgccaga tgtggaagaa actggcagca catttgaaga aaatgcccga
     1861 ttaaaagcag aaacgattgc acaattgctt gatcagccag tcttagcaga tgattcaggt
     1921 ttgaaagtgg atgctttagg cggtatgcca ggaatctatt ctgcacgttt tgcaggggaa
     1981 caaaaaagtg atgcggggaa taacgccaaa ttattatatg aattgacaga tgtgccggat
     2041 gaaaaaagaa cagcacaatt ccattgtacg ttagtattcg cggcacccaa aaaagacagc
     2101 cttgtagtag aagcggaatg gcccggtcga gtagcccgta taccatctgg cgaaaatggc
     2161 tttgggtatg atcctttatt tatcccagaa ggtaagaaac aaacagcagc agaattatcg
     2221 agcgaagaaa aaaacaagat cagtcatcgg gctcaagcaa tgaaaaaatt gagtgccgag
     2281 tggaaacaat ggctggaagg agagagatag atgcggtatt tagtagtaag tgataatcat
     2341 ggagacagag atatcctcgt tgacttagta gatcgttatc gttctgaagt ggatatcttt
     2401 ttccattgtg gtgattcgga attagaagct tctgacccat tatgggatac attccaagtg
     2461 gttcaaggta attgtgatta tggaccagga ttcgaacaga aaaaagtgat ccaaactggt
     2521 caggatacag tattcatgac tcacggtcat ttatcgaatg tccgatttgg tttgacacag
     2581 ttagcaatcg aagctcgagc tgcaactgcc gatatggtgt tttttggtca tacccatcaa
     2641 ataggctgtg agatggatca gaaaatcctg tttttgaatc ctggtagcat ttctcagcct
     2701 agaggcccga tccaaatccc atcatatgct cttatcgaaa gtttagatga ccaaatttct
     2761 gttcaatact ataaccgtgc ccatcaggca gttgaagagt tggccttttc tttttcgaga
     2821 gagcgctgaa taaaaaaact ctaattttta ataaaaataa gctttatttt atgaattttg
     2881 catccggttt cggtaaaata gtagtgttga atgaaataga aatggaggcc atcagatgat
     2941 tggacctata gtaagagaaa tgctgctgga aaatcaggaa acgtttttga ttcctgcaga
     3001 aaatgtggca aatgttatgt atcatcatcc attgtcccat ggtttacttg tgttatcgaa
     3061 ggtcggttat tccaaaattc ctgtattagg aaaagatgat cactttgtag gactagtaag
     3121 tttatcagat gtggtcaata aaatgattga cttgcaaaca atcagcatgg acccgttaga
     3181 aggactaacg gttgctgatg tgacagaaac agatgttccg acaattgatg aaaattggga
     3241 actagaagaa gtactccatt tgttagtcga ttcgcctttt ttaccggttg tcacagaaga
     3301 tcggattttc aaaggaatca ttacaagaaa agagctgctt aaagccgtaa acttcatggt
     3361 gcatgaatta gaacgtcgta atatattgtc tccgaaaatc gaagaattaa aagaacaaat
     3421 cgatatcgtg agctaaagaa cgagaaacta aaagataatc aaaaaataag tagaaaaaac
     3481 agcgtaccag ttttgacttt atcttgaaaa agaggctggg acaaaagcgt ttaactccaa
     3541 gaaataagaa ggaattcacg aaaattgctt t
//
LOCUS       DAYYHB010000162         3553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000162 DAYYHB010000000
VERSION     DAYYHB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.167"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            762..>3553
                     /locus_tag="ACHA6B_002723"
     CDS             762..>3553
                     /locus_tag="ACHA6B_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8950248.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTE
                     LNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVT
                     VTDNRSDADKYEPTVEG"
BASE COUNT         1320 a          527 c          711 g          995 t
ORIGIN      
        1 acatgatgaa tttatggatt atcaaaaatt ggatcagtta attgaaagag aaagctggga
       61 agattattac gtgggtctaa ctttttcaga aaagttaaac aactacaagt taatagatta
      121 taagtatttg agtgaaagca attaattatt catgtagagt actagacact ttaaatatgg
      181 acaattcaat tataaagaaa aagaatcaat aatttaatga ggctgtgaca aatgtcattt
      241 tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc caatcattcg tttaagattc
      301 taggaatcat tcagaaatgc tgtgaaaccg atactttata attataaaga aggtgatttc
      361 aaattccgaa gaaggaattt gaagtcacct ttttacttat gtcacagtct cattcagtaa
      421 tgcgggataa ggctccttat tccgttattt gaatactctg aagtttaatc taagaaaggg
      481 taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat
      541 acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa
      601 aacatgaaga aatgtacttt ttttgttgta atgttcatga aattctctaa gtaaattagt
      661 taacataagg ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat
      721 tcttactagg aaagtaatta ttaagggagt tgatttgaat aatggttagc aagaataata
      781 agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa
      841 gtgttggggt tgcatctgtt ttgattggtg ttggtcttgt cttgggaaca actgaactag
      901 ttaaagcaca agatgaaatt tctccaagca gcccacttga aacagcgata agctccgtac
      961 aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa
     1021 attataactt ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag
     1081 gcaatacaat tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact
     1141 gggaaatccg tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg
     1201 atccaaagaa aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg
     1261 gtagacctac aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg
     1321 gggcagaaac aaccgatacg caaaccgctc ctaatggggt aattacgttt gaaaagagcc
     1381 tagatgaaat ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc
     1441 gaatctactt ggtaagtcat caagacgatg ataaaattgt accgggtatt gatagtacgg
     1501 gctacttttt aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc
     1561 ctgaccagtt taaacatgga tcagtaagtg caaaatatga gaaacctaat actcaaacaa
     1621 aagatggttc aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga
     1681 aaaattataa tttctcatat tcatcttccg caaagggcac gccttggtat gctaattaca
     1741 aaattgatga acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg
     1801 acaaagtaac atatgatgtt tcgtttgaat ccggaacaaa agtagctgat ttagcaatcg
     1861 agcgacggga aggtcatgaa aattatggca taggttcaat aactaataat gatttaacta
     1921 aacttatcga ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat
     1981 tgactaagcc attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa
     2041 ataaaccatt tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat
     2101 taatccagaa cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa
     2161 accctgacga taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag
     2221 aagaagtata tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg
     2281 gaacaggtca caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta
     2341 tcgctccaaa tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg
     2401 gtgtagattc aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag
     2461 gttttgataa accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata
     2521 aacaaaccgt ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa
     2581 acttacctga agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg
     2641 atagtacgaa aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag
     2701 agtttgctgt aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa
     2761 caactccaga aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt
     2821 tagctaaaga tgccttttca atttcagatg tctataataa gtacttcggt aatgcaagtg
     2881 ttgatgcaaa cactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc
     2941 cgattactgg aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg
     3001 atttagcagt aactgtaagt aaagatattt atgaaaatcc aggagaaaac attcctgcag
     3061 gctaccacaa agtaaccttc accgcagggg aaggaacaag tattgaaagt ggaacaacag
     3121 tctttgcagt gaaagacggc gtaagcttgc cagaagataa acttccagtg ttgaaagcaa
     3181 aagatggtta tacagatgcg aaatggccag aagaagcaac tcaaccaatt acagcagatg
     3241 atacagaatt tgtatcaagt gcaacaaaac tggatgataa atctgacgct gacaaatata
     3301 atcctgaagg tcaaaaagtg actacagaat tgaataagga accagacgca tttgagggaa
     3361 ttaagaacaa agaagattta ccaaaagata ctaagtatac ttggaaagaa aaagtagatg
     3421 ttagcgcagc tggaaataaa aaagggactg ttgtagtgac atatccagat ggatcatctg
     3481 atgaagttga agtagatgtt acagtgacag acaatcgttc tgacgccgat aaatatgagc
     3541 caacagtaga agg
//
LOCUS       DAYYHB010000163         3552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000163 DAYYHB010000000
VERSION     DAYYHB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.168"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACHA6B_002724"
     CDS             137..748
                     /locus_tag="ACHA6B_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8950249.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACHA6B_002725"
     CDS             821..1750
                     /locus_tag="ACHA6B_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFM8950250.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACHA6B_002726"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACHA6B_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..>3552)
                     /locus_tag="ACHA6B_002727"
     CDS             complement(2693..>3552)
                     /locus_tag="ACHA6B_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8950251.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKER
                     ELHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEE
                     ILIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRK
                     EVCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRK
                     TIYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVT
                     DTLASMFTE"
BASE COUNT         1170 a          555 c          682 g         1145 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa ga
//
LOCUS       DAYYHB010000164         3502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000164 DAYYHB010000000
VERSION     DAYYHB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.169"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            317..1102
                     /locus_tag="ACHA6B_002728"
     CDS             317..1102
                     /locus_tag="ACHA6B_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8950252.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="ACHA6B_002729"
     CDS             1115..1966
                     /locus_tag="ACHA6B_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8950253.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="ACHA6B_002730"
     CDS             complement(2090..2542)
                     /locus_tag="ACHA6B_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950254.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="ACHA6B_002731"
     CDS             complement(3007..3375)
                     /locus_tag="ACHA6B_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8950255.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1122 a          572 c          686 g         1122 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaaa gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaaa tttacgacat
     2161 gattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 tttgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatccgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata at
//
LOCUS       DAYYHB010000165         3492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000165 DAYYHB010000000
VERSION     DAYYHB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.170"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA6B_002732"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA6B_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8950256.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA6B_002733"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA6B_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          985 a          772 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aa
//
LOCUS       DAYYHB010000166         3372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000166 DAYYHB010000000
VERSION     DAYYHB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.171"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..1200)
                     /locus_tag="ACHA6B_002734"
     CDS             complement(193..1200)
                     /locus_tag="ACHA6B_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8950257.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            1667..3073
                     /gene="lacG"
                     /locus_tag="ACHA6B_002735"
     CDS             1667..3073
                     /gene="lacG"
                     /locus_tag="ACHA6B_002735"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8950258.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3096..>3372
                     /locus_tag="ACHA6B_002736"
     CDS             3096..>3372
                     /locus_tag="ACHA6B_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8950259.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLREL"
BASE COUNT         1098 a          494 c          649 g         1131 t
ORIGIN      
        1 ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg
       61 tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta tttacacaaa
      121 atattttaca ctctcgctcg gctactatgt ggacacaatt atagttttaa agataagttt
      181 caattactta ttttacacct cttctttagg gataaaactt ttcctaacgg tcaattttgc
      241 gccgattaat gttttctttg atatgtcccg cgaatatacc agttgatcac ttaataaatc
      301 tacacaggtt ttagctatct cttcgatatc tatcttaaaa gttgtaagtg gtggtgcaac
      361 gaattttgcg atgtcgtcat cattaatact tattactgcc atatctttag ggatgttaat
      421 acccaattca tggcagccct gaagaacgcc ggcagctata gtgtcagatg gtatgaaaca
      481 tgcctcgggc atatcttcat ttaactcagc tttcatttta agagttaatt gatacccttc
      541 gtcaacagta aatgatcctt gggagaaaat atacttttca ttgagtagtc cgctcttttc
      601 taaatgatat cggaaggcat attcacggct atcaatctct tctttacccg tttctaggtt
      661 aaagaaattt cctccaataa atccaatttt tgtgaaacct tcattaataa agtagttaat
      721 tgcttgtctg gccatttcgt ggaaatcagg ttctacagta tcgaagtgtt caggtaatgg
      781 gtttattcct cggattaccc ccttataccc tttttgattt aagtagtaca attccttttt
      841 tgtcaattgt cctattgcga taaatccagt aatatcgtca ggaatgcttt ttacaccatc
      901 gtttttagag atgaaaatca aatctagatt agctttttta ctataatttt tcaaatcatc
      961 tttgacatgt tgatagtaga catccttttt taactgttcc tctgagatcc aaaaaagaat
     1021 ggcaatcttg tacagagtag tcttaatttt tgtcagatca tatcctaaag atttggctgt
     1081 aacgattatt ttgtttttag tatcagatgt aatagagagt gactcatcac ctttaaaaag
     1141 tcttgatata gtagccgttg agtaaccaga tttttttgca atatctctaa tagtaaccat
     1201 tgttcatcct cctagggata taataatcct tttctttgtt aaagtaaatc gaaactgctc
     1261 ttttagtaac ttaaattact ttttctagca ggaaacgaaa ctctgatatc agcagagagc
     1321 ctaatccatt aacagagaag attgaatcga taatggatag ccacttggag aatgattagt
     1381 aggtgggatt gattttagtg ggaatcagtc cgttttcttg tttcgtgtaa attaaattag
     1441 taaaaagata aatttatttt acaaaaaaac gtttacaaaa cggtttttga atggtatatt
     1501 tattttacat caagggatat aagtgcttgt atatcgcaat aaaatagata ttcgaggtcg
     1561 gtttttcgtt gtgtaaaaaa atttacttat acatgttaaa attatgctga ggtgatttat
     1621 gggttattgg catattgatc aaactaattt tcaaggagag attaaaatga attataaaac
     1681 tttaccagaa gactttatta tgggaggagc tacggcagct tatcaagcag aaggctctac
     1741 aaaagaaggt ggaaaagggc aagtagcgtg ggatactttc cttgtagaaa agggacgcta
     1801 tactgctgat ccagctagtg atttttataa tcaatatcca gttgatgctg aattatgcaa
     1861 aaaatttaaa attgatagta ttaggatgtc tattgcatgg agccgtattt ttccaaaagg
     1921 ctatggggaa gtgaaccaaa caggagtgga atattatcat aaggtatttg ctgaattgaa
     1981 taagcatggt attattcctt ttgtaactct tcatcatttt gataccccta aagaactatt
     2041 tgataatgga gattttttaa atcgagaaaa tgttgaacat ttcgtaaact atgcacagtt
     2101 ctgtttcgat gaattttctg aggttcatta ttggagtaca tttaatgaga tttatccagt
     2161 tgcttcaaat caatatttga tcggatcgtt tcctccagaa gttcaatttg atatagagaa
     2221 gactgtaact tgtttacaca acatgatgta tgcgcacgca aagacagtaa acatttttaa
     2281 agatggtgga tataaagggg aaattggtgt tgttcactcg ctagagacaa aatatccagc
     2341 aagtgacaaa gaagaagata agcatgctgc gaaattagat gatgcgttat caattaagtt
     2401 cttattagat gctacttatt tagggtatta ctcagatgaa acaatggcac atattaatga
     2461 gattttagca gcaaatgggc aatctgcaga tattgattct aatgatttta gagagatgaa
     2521 aaaagcagca aatcgaaatg attttcttgg tatcaataac tatggtagcc attgggttaa
     2581 agagtacaaa ggagaaagca cacttcacta caacactacc ggagaaaaag gtagccaagt
     2641 ctattgcatt aaagggattg gcgagagaat cgtgcgtgag gatcttccga aaaccgattg
     2701 ggattggatc atttatccaa aagggttata tgatttactt gtacgaatcc aagacgaata
     2761 tccaaattat aataaaatat acattactga aaacgggcta gggtataagg ataattttga
     2821 agatgggatt attctagatg atgatcgaat cgattattta cgccagcatt ttaatgcgat
     2881 ttccgatgct atcaaaatgg gagttaatgt aaaggggtat ttcatttggt cattaatgga
     2941 tgttttctct tgggttaatg gatacactaa gcgttatgga ctattctatg tagatttcga
     3001 tacacaaaaa agatatccga aagagtctgc atattggtat aaatttgtta gcgaaacaaa
     3061 aacgataata taaaaatagt taggagggca aggaaatgga taggaaagaa ttgcaattat
     3121 taggatttga aattgttgct tatgcaggag atgccagaag ttcattatta acagtgttga
     3181 aggaagtgag agaaggtaat tttgataatg ttgaacgatt attagcggat gctgatgaaa
     3241 atttaacctt agcgcataac tcacaaacga aatgtttagc tgaagaagcg tcagggaacg
     3301 aaatggaagt aggcgtaatt tttattcatg ggcaagacca tttaatgaca acgattctat
     3361 taagagaact ta
//
LOCUS       DAYYHB010000167         3371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000167 DAYYHB010000000
VERSION     DAYYHB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.172"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHA6B_002737"
     CDS             complement(451..1761)
                     /locus_tag="ACHA6B_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8950260.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHA6B_002738"
     CDS             complement(1773..3191)
                     /locus_tag="ACHA6B_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8950261.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1048 a          681 c          516 g         1126 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa c
//
LOCUS       DAYYHB010000168         3286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000168 DAYYHB010000000
VERSION     DAYYHB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.173"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..503)
                     /locus_tag="ACHA6B_002739"
     CDS             complement(<1..503)
                     /locus_tag="ACHA6B_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8950262.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEF"
     gene            1143..1229
                     /locus_tag="ACHA6B_002740"
     CDS             1143..1229
                     /locus_tag="ACHA6B_002740"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8950263.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1929..2225
                     /locus_tag="ACHA6B_002741"
     CDS             1929..2225
                     /locus_tag="ACHA6B_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950264.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            complement(2529..>3286)
                     /locus_tag="ACHA6B_002742"
     CDS             complement(2529..>3286)
                     /locus_tag="ACHA6B_002742"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8950265.1"
                     /translation="KRQSENKRLNRLISGIYFENKGIYGAPKIHKILVGRGETLSLKK
                     VQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSINQKWVTDITYIHTL
                     KDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTPSENLIIHSDLGSQ
                     YTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYPSKAYENFEAAYLA
                     LFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT         1030 a          614 c          599 g         1043 t
ORIGIN      
        1 ctaaactctt caatacgaac tacataataa ccagtagaac ggtactgcat aagcagtcgg
       61 aatagagttt ttgcatacgt agaacgcaac gacgtgaaag cagataattc aaatctacta
      121 aattcagctg ttaaaccatt aataatatat tctaggtccg gattaacact gacttcaaca
      181 aacttttttg ttttattgat ttcaaaacca gtaaacaaaa caaagtagcg aatagaatcc
      241 tcatcttcag tacggtaagt aagattaagc atatctttat agagcttttc taaatcaccg
      301 acaaaggctt ttgtagcagt catcttataa tcacttaact cttttaattc ctcgaaagaa
      361 aatctaactt ttttcaaacc acgatctttc atacgagcgc ataatgcaaa aaataaatcc
      421 atctgcttag cattgaaatt tttcagcggt actaaattca attcattttg atatttgact
      481 gttaattccg ttttttcatc cattcctttc ttcccccctt acaaataaat aataaccttt
      541 aacaaccaac tcgtaaatag gttggttgtt attatacaaa aggatattag tactcagata
      601 aaaagttgtt agtactcaga taaaaagttg ttagtactca gataaaaagt tgttagtacc
      661 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
      721 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      781 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      841 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      901 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      961 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
     1021 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     1081 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     1141 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     1201 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     1261 aagccccaga gaatccgacc tctctgggga tttcgtgtgt ttcgtgttca cttatttgca
     1321 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     1381 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     1441 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt
     1501 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     1561 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     1621 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     1681 atttggttat cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac
     1741 gtgaccccac ttttaaaaaa agtggttgga taggctgtgc ctatcacgtt tctgaaaaaa
     1801 ataaatgacc ccaaaatacc aatatatagg atagtacaag gatagcaaag gatagtaaaa
     1861 ggatagaggt aggatagcga aaaggatagg atccttttca ctatcctata ttatattgga
     1921 ggatatttat gaatgttaaa attagagatt tagatccaaa attcatttct gaaattgatc
     1981 gaagatgcgt tgaactaagt aatcgtacag gaaataaatg gagtcgtaat gattatttaa
     2041 agctactcgt tgaaaatgat tttgatcgtc ctttgatgga atacaaacaa gaaaagtttg
     2101 accagttatt agaaaagttt tcagaaattc aaagttataa cacaaagata ttagaagaat
     2161 atgttagcca aaataatcgt attatagaaa tcttgattga gcaaaataga ggaagtgaac
     2221 tttgatatgg caaaggttat agacaaacac atttactttc accaaacaca tatcgatgta
     2281 ttggatgaaa ctatttctac tcatgcagag gttaaaaact atagtgaagc tgttcgcttt
     2341 ttatgtatga actggatttg agtcaatacc tcggacacga tttgtgagaa ttttttcgag
     2401 attccagcgg gtgagtatct catcgtttcg accagcggtc aaaccaaaac cgtttgaccg
     2461 ctgatcgaag cgatgaagtt gaaaccctgt ggaaatctcg ccaaaaaaat gtaagtggta
     2521 gctaggaact aagcttgcaa tgccaactcc tccatctgat ttggtgttaa atagttgata
     2581 ctactatgga ttcgcttgcg attgtaaaat ccttcaatgt attgaaataa cgctaaataa
     2641 gcagcttcaa agttttcata cgcttttgat ggatagactt cttctttttt gagtgaggcg
     2701 tgaaacgatt cgataccggc attatcgtag ggacagccct tacgactgaa ggagtgccga
     2761 atgttcaatt cattcagctt tgcctcatag gcttcgctag tgtactgaga acccaaatct
     2821 gagtggatga tcaagttctc ggagggtgtt tggtttaaga gcgcattgtc aagggtttcg
     2881 ataaccaatt ctttcgtcat ctgcttgcca aacttccaac cgatgacttt ctttgtgtgg
     2941 aggtcttgaa tagtggagag gtaggtccag ccatctttta gggtatgaat ataggtaata
     3001 tctgtcaccc atttttgatt gatcgacgta gtggtaaaat cttggtttaa caggttttta
     3061 cgtccttcac ttttaacttt cgcttgtttg ccaggcttgt acttcttgag ggtgactgaa
     3121 cgcaagccta attttctcat cagtatttgt actttcttta aggacaaggt ctcgccacgg
     3181 ccaacaagaa tcttatgaat tttaggcgca ccatagatac ccttgttttc aaagtagatt
     3241 cctgaaatca agcggtttaa gcgtttgttt tctgattggc gcttgg
//
LOCUS       DAYYHB010000169         3204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000169 DAYYHB010000000
VERSION     DAYYHB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.174"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..374
                     /locus_tag="ACHA6B_002743"
     CDS             129..374
                     /locus_tag="ACHA6B_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950266.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(558..1307)
                     /locus_tag="ACHA6B_002744"
     CDS             complement(558..1307)
                     /locus_tag="ACHA6B_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8950267.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1493..2197
                     /locus_tag="ACHA6B_002745"
     CDS             1493..2197
                     /locus_tag="ACHA6B_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8950268.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2237..2794
                     /gene="hxlB"
                     /locus_tag="ACHA6B_002746"
     CDS             2237..2794
                     /gene="hxlB"
                     /locus_tag="ACHA6B_002746"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFM8950269.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2802..>3204
                     /locus_tag="ACHA6B_002747"
     CDS             2802..>3204
                     /locus_tag="ACHA6B_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8950270.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEI"
BASE COUNT         1087 a          464 c          626 g         1027 t
ORIGIN      
        1 aataatatca gttatcaaat ttcctgtaaa ttttcttgaa actacagcat attattactt
       61 caaaacaatc tacatttttt ataaaaaagg aaatgaataa ctgaaaaaat tattatagga
      121 gagaaaaaat gtcaaaatct aaaaaaaata tcaccataat tggtttaggc ttaaccgtat
      181 atttatttac agaatttgtt tatagacatg tgaatttatt tgcagaacct ttatttgtat
      241 tatacgaatc aaattcctca atcgtcagtg aacttgctaa tttaatgata gcattgctgt
      301 ttactcccgc gttgagagtc ataactattt tggtctgttt agtgctattg ttttttgtta
      361 ataaaaagaa ataacgtagt atttcaaaag acaaagaagt gggatgatga ttgctatagt
      421 atgttatacg tggtgctagg taagtgagaa taaacaaaaa aggaaacacg caataaagtt
      481 tatttcaaaa ctttattgcg tgtttctttt ttagattttt tatatctgtt gctttagtag
      541 ttaccaatag ttttcgctta atcaatcaaa acgatttttt gtttattgta ttctttacga
      601 accttttcag gaatttcttc tgaaataaga taggtaaggt cttcaattgg accgacgata
      661 tgattactga tggtgccgaa tttatcatta gttatcatcc caagtacttc agtagaaata
      721 cgtatcatct cacgtttaat gtcggattct tgcatagttg gcacgctcgt tccaacatct
      781 agatctatgt tataaatacc cataatataa aggtcggctc gaatgttttg tagttgctta
      841 taagcttgta ccccaatatt gatcatagat tgtttataaa aagtaccgcc aatattgata
      901 atttccaaat taggatgctg tgctaatgct atggcaatcg gcgggctatt ggtaatgatt
      961 gttgcacgaa aatcttgtgg gatttctttt gctaaagcca agttagttgt acttccatca
     1021 atgatgatga cagattcttc ttttaaaaaa gggagggctt tatgagctaa tgcagttttt
     1081 tgttcgctag ctacagtttc tcggtagtta aaattggtaa ttggcggacc aacacgtaaa
     1141 gcgccgctgt gtactctttt taagagacct tggctatcca aatctttcaa atctcgtcga
     1201 atcgtgtctt cggatacatt gaaacgttga cttaaatcac tagcgataac tttttgttct
     1261 gcgtttaata aatctagaat tttttgatga cgttctaatt tcaacatgtt gcgtgctcct
     1321 tcttatttat tcttgtttat catgtcattt atgcgttttt atgtcaataa tttccgtttt
     1381 taatacacta aaaaggcata aacaagcaaa aacaaaatat ttttatgcag ttttatgttg
     1441 actagtattt gttaagcggt tacaatgagg gggtaattaa aggagggaat gtatggagaa
     1501 aaaaattatt ttgtcgcctt cacttatgtg tggggatcta gcaaatttgg aaggaagcat
     1561 ccaagaacta gaaagtattg ggatggattc cttgcatatt gacgtgattg acggttcttt
     1621 tagtcccagt atgcctttag ggatcgaaac aatcaaaaga attagagaaa tcacttcaat
     1681 gaattttgat gtacacatca tggctaagga taacgataac gaatttttta ttggagaaat
     1741 gttgaaaatt ggcgtagaga gcattacttt tcattatgaa acaagcctcc atattgatcg
     1801 atatatccag ctcatcaaga attctggaac aaaagtaggg atagctttga atccagcaac
     1861 tccgttaaat gtattacaat atattttacc cgagattgat aggatttgtt tgatgctgat
     1921 taatccaggt tttgcaacaa acaagtcaga aaaacaagtt tcttacgcgc taaaaaaaat
     1981 tacagatctt tgccagttag taaatgagaa agagttagca gtagatatcc aagtggatgg
     2041 acgggtatcg ttagaaacga tcccgggatt attagatgca ggcgcaacaa atctggtatt
     2101 aggaagtaca agtttgtttc ttaaaggaaa tacattagca gaaaatcaaa aactacttta
     2161 tcaagcgatg aagcaatcag ttaggaaaaa tcaatagatg tagtggtcct gaataataga
     2221 aatggagggg aaatagttga gttataaggc ggcgaaacaa aatattgtaa gagaaattac
     2281 tgaaacatta gaccgtattt cttcaaaaga tgtggaacaa ttgataaaga aaattgaaaa
     2341 agcggaacga gtttttttcg tgggggttgg cagagtactg ttagcgctag aaggaatggc
     2401 aaaacgctta gggcatctag gaattcagac agtagtcgta ggacaaatca ctgaaccggc
     2461 aattacagat aaagatttat tgattgttgg ttctggtagt ggggaaagtg ctttcccact
     2521 aactattgca aaaaaagcaa aggaatttgg gtcccaagtt gcccatatag gatcaaaccc
     2581 agacagcaca atgaaagaat attcagatct ttttattcgt atacccgtac aaacaaaatt
     2641 agcgttacca gaggaaattt cttctataca gccaatgact agcctgtttg aacaaagtgt
     2701 tcttttgtta ggagatagta ttgctttagt gatgatagaa gaaaaagcaa ttgatatgtc
     2761 aaagttgtgg caataccatg ctaacttaga gtaggtgaaa aatgaaagcg gtacattttg
     2821 gagcagggaa catcggcaga gggcttattg gacaaattct acatcagaat ggattcgatc
     2881 tatgttttat tgatacgaat cctgaactga tccatcaatt aaatcaagat aagggttata
     2941 ctatcgaaat tattgatgaa atgaacagga gtgatttcat tgatcatgtt gtggcactga
     3001 atagtttatc cgagctagaa gaggtgatta cagcaacaaa aaatgcagat atcttaacaa
     3061 catctgttgg cgcaataaat ttagtaaaaa ttgcaccgat cattgcgcaa gccttaagta
     3121 agcgatttga acaaaagcat agcatcaata tcgtagctaa tgaaaatgtg ataaatgcaa
     3181 gtgatgtgtt gaaaaaagaa attt
//
LOCUS       DAYYHB010000170         3023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000170 DAYYHB010000000
VERSION     DAYYHB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.175"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHA6B_002748"
     CDS             complement(287..649)
                     /locus_tag="ACHA6B_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950271.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHA6B_002749"
     CDS             complement(723..1103)
                     /locus_tag="ACHA6B_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8950272.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHA6B_002750"
     CDS             1185..2360
                     /locus_tag="ACHA6B_002750"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8950273.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHA6B_002751"
     CDS             complement(2437..3021)
                     /locus_tag="ACHA6B_002751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8950274.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          498 g         1005 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tat
//
LOCUS       DAYYHB010000171         3003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000171 DAYYHB010000000
VERSION     DAYYHB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.176"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..874
                     /gene="agaS"
                     /locus_tag="ACHA6B_002752"
     CDS             <1..874
                     /gene="agaS"
                     /locus_tag="ACHA6B_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-6-phosphate ketose isomerase"
                     /protein_id="HFM8950275.1"
                     /translation="EPEEYLIKEEPTLLVSFARSGNSPESLAAVEIANQVVDTIHHLS
                     ITCAKEGKLAQISQADSKSFLFLMPVRSNDDGFAMTGSFSCMTLGALLTFDQTALAEK
                     QNYVETLNALGNEVIAREHDIEEIVALDFERIVYVGSGSLAGLTREAQLKILELTAGK
                     IATVFDSSMGFRHGPKSFINEKTIMFGFVNNDPYTRDYDLDILEEVHEDEIASGVFAI
                     TQPGKRNFSGDTFEFATGTSLLPDAYLALGYVMVAQTVALLTSVKVANTPDTPSPTGT
                     VNRVVKGVTIHDYK"
     gene            897..1904
                     /gene="lacD"
                     /locus_tag="ACHA6B_002753"
     CDS             897..1904
                     /gene="lacD"
                     /locus_tag="ACHA6B_002753"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8950276.1"
                     /translation="MLTLTTGKKAAMDRLSTKDGIISALAIDQRGALKKMIKALNVEP
                     TDKHIEHFKELVSSELTPYASSILLDPEYGLPAAKVRHADAGLLLAYEKTGYDATTPG
                     RLPDLLADWSVLRLKEQGADAIKFLLYYDVDEDPAINHQKHVFVERLGSECAEEDLPF
                     YLELVSYDAQISDAGSFDYAKVKPHKVNDMMKEFSKPQYKVDVLKVEVPVNMNFVEGF
                     GTGEIAYTKEEAANYFLEQSQATDLPFIFLSAGVSTELFQQTLVFAKEAGSTFNGVLC
                     GRATWKNGVKPFIESGEAAARDWLQSEGRENIESLNNMLKQTASSWHDKVQVAEEEAV
                     N"
     gene            1916..2878
                     /locus_tag="ACHA6B_002754"
     CDS             1916..2878
                     /locus_tag="ACHA6B_002754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8950277.1"
                     /translation="MIVTVTMNPSIDISYPLEKLKLDTVNRTDQVSKTAGGKGLNVTR
                     VIHDLKGDVLATGVLGGFHGAYISEELKKAGIKQAFTEIQEESRDSIAILHEGNQTEI
                     LEAGPTVSEAEQQAFLENFQELVKEAEIVTISGSLAKGLPLNFYQRLVQLAEEQVVKV
                     LVDTSGESLRQVLTGEHKPFLIKPNLEELEALLGQPFSIDDLEELQSALAQPLFTGVE
                     WIVVSLGKDGALAKYRDSFYRVTIPTIKTVNPVGSGDATIAGFAYGLSQKMSVTELLK
                     LCMAAGMANAQEVRTGHVNLANVDMHFEEINVRKASRTRSAFEQ"
BASE COUNT          933 a          565 c          712 g          793 t
ORIGIN      
        1 ggagcctgaa gaatatttaa tcaaagagga accgacctta ctggtatcct ttgccagaag
       61 cggaaacagt cctgaaagct tggcagcagt tgaaatcgca aatcaagtcg tggatacaat
      121 ccatcattta tcaatcacct gtgcaaaaga gggaaaattg gcgcaaatca gccaagcaga
      181 ctccaagagc tttttattct taatgcctgt tcgttcgaat gatgacggtt ttgccatgac
      241 tggcagcttc tcctgtatga cactaggcgc gttgctgaca tttgatcaaa cagcactagc
      301 ggaaaagcaa aactatgtag aaacattgaa cgctttagga aacgaagtga tcgcccgtga
      361 gcatgacatc gaagaaattg tcgcacttga ttttgaacga attgtttatg tcggttccgg
      421 cagtttagca ggcttgacca gagaagcaca attaaaaatc ctagaactga ccgctggaaa
      481 aatcgcgaca gtctttgatt catcgatggg ctttagacac ggaccaaaat catttatcaa
      541 tgaaaaaact attatgttcg gttttgtcaa taatgatcca tacacgcggg attacgattt
      601 agatatctta gaagaagttc acgaagatga aatcgcttcc ggtgtctttg ctatcactca
      661 acccggaaaa cggaatttct ctggggatac gttcgagttt gcaactggga cttctttact
      721 tccagatgcc tatttagcac taggctatgt catggtagca caaactgtcg cgcttttaac
      781 atcggtgaaa gttgcgaata cacctgacac gccttcacca acgggaacag tcaatcgagt
      841 ggtaaaaggc gtaacgattc atgactataa ataggaatta actgggagga agaaacatgc
      901 ttacattaac aacaggtaaa aaagcggcga tggaccgttt atcaacaaag gatggcatta
      961 tcagtgcctt agcaattgat caacgtgggg cgttaaaaaa aatgatcaaa gcgttaaatg
     1021 tcgaaccaac ggataagcat attgagcatt ttaaagaatt ggtatcgagc gagctgacac
     1081 catatgcgtc ttcgatctta ttggaccctg aatacggtct gcctgcagca aaagtacgtc
     1141 atgcagatgc aggtctattg ctggcatatg aaaaaacagg ctacgatgca accacaccag
     1201 gacgtttacc tgatctatta gcggattggt cggttttacg actaaaagaa caaggtgccg
     1261 atgctatcaa gtttttactt tattatgatg tggatgaaga tcctgcgatc aatcatcaaa
     1321 agcatgtctt tgtcgaacgg ttaggcagtg aatgtgcaga agaagatttg ccattttact
     1381 tagaattggt ttcttatgat gcgcaaattt cagatgctgg ttcatttgac tatgccaaag
     1441 tgaaaccgca caaggtcaat gatatgatga aggaattctc aaaaccgcaa tacaaagtgg
     1501 atgttctaaa agtggaggtc ccagtgaata tgaatttcgt tgaaggcttt ggtactggtg
     1561 aaatcgctta tacaaaagaa gaagcggcaa actatttctt agaacaaagc caagcaacgg
     1621 atttaccatt tattttctta agcgcaggtg tttcaacgga gctattccaa caaacattgg
     1681 tatttgctaa agaagctggc tccaccttca atggtgtgct ctgtgggcgt gccacctgga
     1741 aaaatggtgt aaagccgttt attgaatcag gtgaagctgc tgctcgtgac tggctgcaaa
     1801 gcgaaggtcg tgaaaatatc gaaagcttga acaacatgtt aaaacaaacg gcaagctcat
     1861 ggcatgacaa agttcaagtc gcagaagaag aagcggtgaa ctaggaggcg agctcgtgat
     1921 agtaacagta acgatgaacc cgtccattga tatttcttat ccactggaga aactgaagct
     1981 agacacggtc aatcgaaccg atcaggtgag taaaacagct ggcggaaaag gacttaatgt
     2041 cacgcgtgtc attcatgatt tgaagggcga tgttttagca acgggtgttt taggcggttt
     2101 tcatggcgct tatatctcgg aagaattaaa gaaagcaggt atcaagcagg catttacaga
     2161 aatccaagag gaaagtcgag attcaattgc gatcttgcat gaaggaaatc agacggaaat
     2221 attagaagct ggtccgacag tttcagaagc ggaacagcag gcttttttgg aaaattttca
     2281 ggagttggtt aaagaagccg agattgtcac catttcaggc agtctggcta aagggctccc
     2341 attgaatttt tatcaaaggc tcgttcaatt agcggaagaa caagtggtaa aagtccttgt
     2401 ggatacctct ggtgaaagcc tgaggcaggt gttaaccggt gagcataaac cctttctgat
     2461 taagccaaac cttgaagagc tagaagcttt attgggtcag ccgttttcaa ttgatgactt
     2521 agaagagctg cagtcagctt tagctcagcc tttatttact ggtgttgagt ggattgttgt
     2581 gtcactaggc aaagatgggg cgcttgcaaa atatcgtgat tctttctatc gagtaacgat
     2641 tccaacgatt aaaacagtta atccagtggg gtcaggtgat gctactatag ctgggtttgc
     2701 ctatggctta tcacaaaaaa tgagcgtaac agaactgctg aagctttgta tggcagccgg
     2761 tatggccaat gctcaagaag tgcgaacagg tcatgtcaat ctagcgaatg tggacatgca
     2821 ttttgaagag atcaacgtaa gaaaggcttc gcgaactcgc tcagcttttg agcaataaga
     2881 ccaaaatatt tctaaattgc caagaaacac agtgatcgaa gagaactgat gttgattggt
     2941 acctacttgg tgcttctcaa atttcgaaat atttttgctt attgccgaaa aagctagttc
     3001 gtg
//
LOCUS       DAYYHB010000172         2990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000172 DAYYHB010000000
VERSION     DAYYHB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.178"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1712
                     /gene="alsS"
                     /locus_tag="ACHA6B_002755"
     CDS             48..1712
                     /gene="alsS"
                     /locus_tag="ACHA6B_002755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8950278.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1725..2435
                     /gene="budA"
                     /locus_tag="ACHA6B_002756"
     CDS             1725..2435
                     /gene="budA"
                     /locus_tag="ACHA6B_002756"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8950279.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2565..>2990
                     /locus_tag="ACHA6B_002757"
     CDS             2565..>2990
                     /locus_tag="ACHA6B_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8950280.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
BASE COUNT          996 a          502 c          684 g          808 t
ORIGIN      
        1 atttctaact aataattaaa aataaagcat aaaaaggacg atgttcaatg gacgaaaaaa
       61 tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa tcatcatgtt gattatgtat
      121 tcggtatacc aggtgcaaaa atcgatggtg tatttaatga attagaggat caaggtcctg
      181 aattaatcgt gactcgtcac gaacaaaatg ctgcatttat ggcacaagca atcggacgta
      241 ttacaggaga gccaggagta gttatcgcaa caagtggtcc tggtgctagt aacttagcta
      301 caggtttggt aacagcgaca gcagaaggtg acccagtttt agcaattgcc ggacaagtaa
      361 aacgaagcga tctattgaag ttgacccatc aaagcatgga taatgcagca ctgttccagc
      421 caatcacaaa atacagtgca gaaatccaag atccagaaac aatttcagaa gtgattgcca
      481 atgcttatcg tatggctaag agttcaaaaa aaggcgcgag ctttatcagt atcccgcaag
      541 atgtagtgga tgcacctgtc caagggaatg tcatcaagcc attgagcgat cctaaattag
      601 gttctgcttc tgcagatgat atccgttacc tagctgaacg cattagagaa gcaaaactac
      661 cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga aacgttagcc atcaggcaat
      721 tagttggaaa gacagcattg cctgttgtag agacattcca agcagctgga gtgatctcac
      781 gtgaattaga agcgcatttc tttggtcgtg tgggtttatt tagaaaccaa ccaggagata
      841 tgcttttgaa acgaagtgat ctagttattg ctattggcta tgatccaatc gaatatgaag
      901 caagaaactg gaatgcggaa aaagatgcgc ggatcattgt gatcgatgaa gcgccagctg
      961 aaatcgatcc ttttatgcag cctgaaagag aattgatcgg tgatatctca gccactcttg
     1021 atttattgac aggttcatta gagcctcaac aagtgtcaga agatgcaaaa gaatatttag
     1081 catctcttca agcagaatta acggagcggg acattgttca atcaaaagga gaagcaggta
     1141 tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa agtaacagat gatatgactg
     1201 ttaccgtaga tgtaggtagt cattatattt ggatggcacg ccactttaga agttatgaac
     1261 ctcgtcattt gttattcagt aatggtatgc aaacgcttgg tgttgcattg ccatgggcga
     1321 tttctgctgc attggtgcgt ccaaatacac aaatcgtatc agtttccggc gatgggggat
     1381 ttttattctc tgcccaggat ttagaaacgg cggtacgtaa aaaattgaat atcgttcatt
     1441 tgatttggaa tgatggacac tacaatatgg tagagttcca agaaaaaatg aaatatcagc
     1501 gggcgtcagg tgtagacttt ggtccagttg attttgtgaa atatgcagaa gcttttggcg
     1561 ctaaaggaat ccgagctaca agtgtagagg aattagaaaa agctttagaa gaaggatttg
     1621 cgacagaagg acccgtaatt attgatattc cgattgatta ccgtgataat gagaaattgg
     1681 gagaaacaat cttaccagat caattctatt aaggaagtgt cgacatgact gaaaaaatct
     1741 tataccagca cgggacatta ggagctttga tggctggctt gatggatgga acagaaacga
     1801 ttgctcatat attagaaaaa ggaactctgg gattgggaac attgcatggg cttgatggtg
     1861 aagtcatctt tttagatggc gtagcttatc aaggaagatc agatggctca gttgcccaat
     1921 tagatggaag tgaacttacc ccctatgcag caattacaga ttttacgcca gatactagtt
     1981 tttctgtctc aaaaactgca gatggagaac aattgaaaaa agacatatta gtaagagaag
     2041 ccggggaaaa cctatttttg gcagtgaaga taactggatt attcaaaaat atgcacatcc
     2101 gtatcatgcc gaagcaagaa aaaccttaca gaagactagc aaaaatttca gagtctcaac
     2161 cagaattcca acaaagcaat gtccaaggaa cattagtcgg gttctttacg cctgaattat
     2221 tccaaggagt tgctgcagcg ggatttcact tgcattttat cgatgataca catacatttg
     2281 gcggacatgt aatggatttt gaagtggctg aaggaaaagt tgagattagc cgcatctcgt
     2341 cccttgtaca gcattttcca gttgatgatc cgacttttgt tcacgaaacg attgattatg
     2401 ctgatttagc agcagaaatc caacaagcag aatagaaaaa gacagcgaga tgcgttcaag
     2461 gtgattaacc tgacgtatct cgtttttttg aggaggaaac ctagcgttta caaaaaaact
     2521 gagctataat cttctccata gatcaaaaag gagcggtttt tatcatgtgg acagaaaaac
     2581 agaaaaaaga tgcatacgaa attttattat tacagcaaca aggattatta gaagcagaag
     2641 ataactggtt agcaaattta gcaaattcat ctgcattgtt gaatgagact ttgcctgaga
     2701 ctgtctttgc aggatattat ttattcgatg gtaatgaatt gattttagga ccgttccaag
     2761 ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt ttgtggagaa tcagctgaaa
     2821 aacaagaaac attgatcgtt gatgatgtca aaacccatgc aaattacatt tcctgcgatt
     2881 cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga aggtaattta atcggtgttt
     2941 tagatatcga ttgcggagta acgaaagggt atgatgaaat cgatcaagca
//
LOCUS       DAYYHB010000173         2797 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000173 DAYYHB010000000
VERSION     DAYYHB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.179"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACHA6B_002758"
     CDS             <1..712
                     /locus_tag="ACHA6B_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950281.1"
                     /translation="VVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGK
                     RKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLS
                     KVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIY
                     FADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRT
                     PLEVFLSYVSIDDLSNLI"
     gene            845..1051
                     /locus_tag="ACHA6B_002759"
     CDS             845..1051
                     /locus_tag="ACHA6B_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8950282.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1062..2081
                     /locus_tag="ACHA6B_002760"
     CDS             1062..2081
                     /locus_tag="ACHA6B_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8950283.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2270..2343
                     /locus_tag="ACHA6B_002761"
     tRNA            2270..2343
                     /locus_tag="ACHA6B_002761"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2304..2306,aa:Ile,seq:cat)
BASE COUNT          938 a          460 c          554 g          845 t
ORIGIN      
        1 ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg
       61 ttctgctcgt accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc
      121 gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg
      181 ccgctctatt catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct
      241 tgaaggtgac actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg
      301 attatcaaaa gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa
      361 aaaattaaat caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga
      421 ttgtggaaag gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta
      481 tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt
      541 acgtagagat ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca
      601 atctgtcgca tctaaacgaa ataatattcc tagaaaatca ctggattatc gaacaccttt
      661 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
      721 aaagataaaa ttgaacaact ggacgggcaa attgatgcct tgctttcaga aagagaaata
      781 ttgatagaag aaaacaagcg attgaaaaag aaagtagccc aaccaatgaa gggaggtgga
      841 tagtatgaac aatagaacct ttgatatttt gaaatggatc gctatagtag ttatccccgc
      901 tttagcgact tttgtcggtc ttgtcggaaa aggtgttaac tggcggtaca cagatataac
      961 ggtgatgatt attacaggtt ttggagcatt tctagggagt attttaggtg tatcaaatcg
     1021 aacgtataag aaaatttcat cagataaata ggaggataga tatgaaaaag aaaattacta
     1081 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     1141 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     1201 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     1261 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     1321 tttatcaagt cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcca
     1381 acagtcctgt gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact
     1441 acaaggcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacgt
     1501 tggacactcc ttataaccaa ccgggaatca aatcgcattt atgggtaaca caaaacatct
     1561 ggggcgatca tacagatcct tacggttatc tttctgaaat gggcgtaagt aaagaaaaat
     1621 tagcatatga tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc
     1681 ctgtcattga tccaacacga gctggtgcag ctaatcctac actgacagat ggaacgaatt
     1741 acgcccacat tgatcagttt ggagaaatcg aaaatgcaaa tttgcatgta gctggatggc
     1801 acattgctaa ctataaatac gagtatatct tcattatgga ttacaatact gggaaagaat
     1861 tagctcgagt aagagctgat ggaatttata gatcagatgt aaatcaagct tataatactt
     1921 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     1981 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     2041 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     2101 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     2161 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     2221 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     2281 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     2341 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     2401 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     2461 taatgaaagg atgtttcaca tagttatatt tctgtatatt tgaaaagttt tactttgatt
     2521 tttaaataga aagacatttg ggttaaattg tgagatgata ataaagaaga gtttaaagcg
     2581 caccccaagc cacttcccca taagtgtgtt acgctttaaa ctcttttata tttgaagcta
     2641 ttaaaaggca taccatattt ttgaaaaaaa gtgagaaaaa aggcttacaa ttggagtggt
     2701 agttaattag tgacttattt ttgattttat agcactgata ctataaaata tagatatcat
     2761 catattacac aatcttaata ctaacttaaa aaatatc
//
LOCUS       DAYYHB010000174         2782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000174 DAYYHB010000000
VERSION     DAYYHB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.180"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..83)
                     /locus_tag="ACHA6B_002762"
     CDS             complement(<1..83)
                     /locus_tag="ACHA6B_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HFM8950284.1"
                     /translation="MVIYYLTGGNNSMSRFCKFGKLHVTKG"
     gene            complement(52..789)
                     /gene="erm(B)"
                     /locus_tag="ACHA6B_002763"
     CDS             complement(52..789)
                     /gene="erm(B)"
                     /locus_tag="ACHA6B_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8950285.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(914..997)
                     /locus_tag="ACHA6B_002764"
     CDS             complement(914..997)
                     /locus_tag="ACHA6B_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8950286.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(1046..1129)
                     /locus_tag="ACHA6B_002765"
                     /pseudo
     CDS             complement(1046..1129)
                     /locus_tag="ACHA6B_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            complement(1539..2333)
                     /locus_tag="ACHA6B_002766"
                     /note="aph(3')-IIIa"
     CDS             complement(1539..2333)
                     /locus_tag="ACHA6B_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8950287.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2426..2677)
                     /locus_tag="ACHA6B_002767"
                     /pseudo
     CDS             complement(2426..2677)
                     /locus_tag="ACHA6B_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          839 a          564 c          447 g          932 t
ORIGIN      
        1 ttccctttag taacgtgtaa ctttccaaat ttacaaaagc gactcataga attatttcct
       61 cccgttaaat aatagataac tattaaaaat agacaatact tgctcataag taacggtact
      121 taaattgttt actttggcgt gtttcattgc ttgatgaaac tgatttttag taaacagttg
      181 acgatattct cgattgaccc attttgaaac aaagtacgta tatagcttcc aatatttatc
      241 tggaacatct gtggtatggc gggtaagttt tattaagaca ctgtttactt ttggtttagg
      301 atgaaagcat tccgctggca gcttaagcaa ttgctgaatc gagacttgag tgtgcaagag
      361 caaccctagt gttcggtgaa tatccaaggt acgcttgtag aatccttctt caacaatcag
      421 atagatgtca gacgcacggc tttcaaaaac cactttttta ataatttgtg tgcttaaatg
      481 gtaaggaata ttcccaacaa ttttatacct ctgtttgtta gggaattgaa actgtagaat
      541 atcttggtga attaaagtga cacgagtatt cagttttaat ttttctgacg ataagttgaa
      601 tagatgactg tctaattcaa tagacgttac ctgtttactt attttagcca gtttcgtcgt
      661 taaatgccct ttacctgttc caatttcgta aacggtatcg gtttctttta aattcaattg
      721 ttttattatt tggttgagta ctttttcact cgttaaaaag ttttgagaat attttatatt
      781 tttgttcatg ttatcactcc tgaagtgatt acatctataa acaaatacag aagttaaacg
      841 atttgtttgt aattttagtt atctgtttaa aaagtcataa gattagtcac tggtaggaat
      901 taatctaacg tatttattta tctgcgtaat cactgttttt agtctgtttc aaaacagtag
      961 atgttttatc tacataacgc atttggaata ccaacatgac gaatccctcc ttcttaatta
     1021 caaattttta gcatctaatt taacttcaat tcctattata caaaatttta agataatgca
     1081 ctatcaacac actcttaagt ttgcttctaa gtcttatttc cataacttta gggttaacca
     1141 tacgcaagac caatcactct cggacaatac tcatgatttt aatgataaaa tccatgttaa
     1201 acccatagat aagaaataca cctgcaataa ccgttacctg tttgtgccaa tttaaaaatg
     1261 caccactttt ttataattaa aacggttgaa aactgtacca ctaataactc acaatagaga
     1321 gatgtcaccg tcaagttaaa tgtacaaaat aacagcgaaa tttttaaatc tatttcttat
     1381 cgatacaaat tcctcgtagg cgctcgggac ccctatctag cgaactttta gaaaagatat
     1441 aaaacatcag agtatggaca gttgcggatg tacttcagaa aagattagat gtctaaaaag
     1501 cttgtagtta aagcttttta gacatctaaa tctaggtact aaaacaattc atccagtaaa
     1561 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     1621 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     1681 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     1741 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     1801 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     1861 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     1921 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     1981 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     2041 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     2101 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     2161 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     2221 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     2281 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     2341 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     2401 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     2461 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
     2521 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
     2581 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
     2641 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gataacccgt ggtgctagtt
     2701 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata
     2761 gtagtctcaa aactcccaag aa
//
LOCUS       DAYYHB010000175         2738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000175 DAYYHB010000000
VERSION     DAYYHB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.182"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACHA6B_002768"
     CDS             171..515
                     /locus_tag="ACHA6B_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8950288.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACHA6B_002769"
     CDS             516..935
                     /locus_tag="ACHA6B_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8950289.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="ACHA6B_002770"
     CDS             complement(1027..1377)
                     /locus_tag="ACHA6B_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950290.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACHA6B_002771"
     CDS             complement(1343..2674)
                     /locus_tag="ACHA6B_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8950291.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1015 a          495 c          527 g          701 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcat
//
LOCUS       DAYYHB010000176         2736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000176 DAYYHB010000000
VERSION     DAYYHB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.183"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1600
                     /locus_tag="ACHA6B_002772"
     CDS             149..1600
                     /locus_tag="ACHA6B_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8950292.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="ACHA6B_002773"
     CDS             1949..2581
                     /locus_tag="ACHA6B_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8950293.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1037 a          390 c          527 g          782 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataat
//
LOCUS       DAYYHB010000177         2618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000177 DAYYHB010000000
VERSION     DAYYHB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.184"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..611
                     /locus_tag="ACHA6B_002774"
     CDS             105..611
                     /locus_tag="ACHA6B_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8950294.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            628..1527
                     /locus_tag="ACHA6B_002775"
     CDS             628..1527
                     /locus_tag="ACHA6B_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8950295.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1614..2498
                     /locus_tag="ACHA6B_002776"
     CDS             1614..2498
                     /locus_tag="ACHA6B_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8950296.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          956 a          484 c          514 g          664 t
ORIGIN      
        1 ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag
       61 cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat
      121 ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg
      181 attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag
      241 atgtgatgca agatagtttt cataaagccc tgaactattt tgaaaaagaa aaagagatcg
      301 agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca
      361 gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt
      421 tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag
      481 agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgcg actatcttgg
      541 atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa
      601 tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta
      661 aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt
      721 tttattatcg aagagcaatc atttataaaa gcaaagcgga gaaagagacg tctccaaggg
      781 ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa
      841 gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc
      901 gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa
      961 aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa
     1021 gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt
     1081 ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc
     1141 acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac
     1201 agtgtccagc tctcacttcc acttttattc aaagatgaca cctatctatc tgttttgaca
     1261 aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg
     1321 actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga
     1381 agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct
     1441 cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg
     1501 gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt
     1561 tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa
     1621 gaaattattt agttatcagt attattgctt tatccgtgct tttgtttttc gaaattactt
     1681 cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac
     1741 aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg
     1801 tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcagtcatca caatcaacac
     1861 aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg
     1921 atggaccagg agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc
     1981 atgccacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga
     2041 cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc
     2101 tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaagtca
     2161 ctggcaaaac ccccactttt gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa
     2221 cgatcatcca gcgaccgatc atcgaatggt ctgttgattc agaagattgg aagacaagaa
     2281 atcctgattt gattttgcag aagatccaag caacagtata cgatggggca attattttat
     2341 tccatgatat ctatcctgaa acgattcgtg ccgttcctca agtgatcgat tatctgaaag
     2401 agcaagggta tcgcatcacg acagttgggg atttattagg gcatccaacg gctgtcgaga
     2461 actattatgg cagaaacgat catcggccag ttcagtaagt gagacaagta tctttaataa
     2521 ataacacatt ttaataagta gttgaacaca taactacctt acttgcaaag gcttaacaat
     2581 atctatacag gtacaacaaa aaactgtcca ccaatttt
//
LOCUS       DAYYHB010000178         2585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000178 DAYYHB010000000
VERSION     DAYYHB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.185"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1026..1544)
                     /locus_tag="ACHA6B_002777"
     CDS             complement(1026..1544)
                     /locus_tag="ACHA6B_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8950297.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1911..2159
                     /locus_tag="ACHA6B_002778"
     CDS             1911..2159
                     /locus_tag="ACHA6B_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950298.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          842 a          466 c          368 g          909 t
ORIGIN      
        1 tatatagttg gattaagata acgactattt attttatcat atttttacta taccctatac
       61 ttttacttgg tatatttatg aataatcgca aatttacccc tatactacta gtttgtaagt
      121 tagattaata aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa
      181 attgatggta tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg
      241 acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg
      301 aaatgaaaga ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa
      361 actaatgttc ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga
      421 cgatttcacc ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat
      481 cagtaatagg ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa
      541 tctgtatcgt atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata
      601 agcgttgaat cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt
      661 accggacttg ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc
      721 ggtactatcg gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct
      781 cctaagtccc tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg
      841 tttatctgtt ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta
      901 ggggagtgca cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc
      961 gtatgttctt actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg
     1021 ggaatttaca aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga
     1081 gcaactgtat ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg
     1141 tctgcccatt ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc
     1201 atgttaatat catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt
     1261 gcataggtat ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca
     1321 gaaggcgttt taacagataa tccatttctt tcatcatttt cccattttag aattaaattc
     1381 gtccaaccaa cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt
     1441 ttatccttca attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta
     1501 tttatttctt tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa
     1561 attcctaatt gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct
     1621 acaatgcata tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat
     1681 cccaacgtct catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata
     1741 tttcttttgt agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc
     1801 atgttaacta ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag
     1861 tttagttaag tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac
     1921 tcaaaatgct ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa
     1981 aatattatga agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc
     2041 tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt
     2101 cacgcctcgc tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg
     2161 aaattgaagc tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag
     2221 atattcttct agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg
     2281 aatttactaa aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg
     2341 gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa
     2401 ttatagaaaa ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct
     2461 tattctgaaa cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag
     2521 tactatttta tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac
     2581 ttagc
//
LOCUS       DAYYHB010000179         2505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000179 DAYYHB010000000
VERSION     DAYYHB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.186"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..822)
                     /locus_tag="ACHA6B_002779"
                     /pseudo
     CDS             complement(1..822)
                     /locus_tag="ACHA6B_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1005..1856
                     /locus_tag="ACHA6B_002780"
     CDS             1005..1856
                     /locus_tag="ACHA6B_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950299.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            1877..2089
                     /locus_tag="ACHA6B_002781"
     CDS             1877..2089
                     /locus_tag="ACHA6B_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950300.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          859 a          381 c          481 g          784 t
ORIGIN      
        1 aattgaaatc catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt
       61 ttaggcctct ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat
      121 tactgatctg tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata
      181 gatttttcgg tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc
      241 taccacacgg tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag
      301 cacttttatg tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga
      361 attggctata atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc
      421 tttcttgatg tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata
      481 gccccttttt gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac
      541 gaccaacaat cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt
      601 gttcctcagg taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt
      661 gatagtactc taaggctgat tttccttgct tgaagaacag gtatacttta tcgtttgtct
      721 tgaacgattc aagaaatgcg cagttttcgc aacagattga tttattttga aataagactc
      781 tatcattaca agttcatccg ttgtaagatg ggtataggtc atttgtactc actccttata
      841 attctttgga cggaactatt ttgagtgtag cacaaatgac ttttttagtt gtctagctta
      901 attttacaat cggcgtactt aaaaaattgg ttcattgagc aacctatcgc tgctaaagta
      961 gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa tcggacgact
     1021 ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat
     1081 tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt cttgatttaa
     1141 ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat cccaatagga
     1201 ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg ctattgtcta
     1261 tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga aaacttttca
     1321 aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata aaacaaattg
     1381 aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt attcactttg
     1441 taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc gggtatgtat
     1501 ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg attccgacac
     1561 ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc aatacggata
     1621 tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt ttaaaaaatg
     1681 gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat gaagtcgaag
     1741 caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt cgtcaaaaca
     1801 aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat aaataattat
     1861 ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg gcatggagtt
     1921 aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc agttactatg
     1981 tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat taacgaatca
     2041 aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat ggctacttat
     2101 agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga acgtctctaa
     2161 aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc
     2221 aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata tttaaaaata
     2281 ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc aaaaaggata
     2341 ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa gaataagaaa
     2401 tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaacggt tctgttgcaa
     2461 agttttaaat ctactatcaa ataaggtaga ataatagaaa aagat
//
LOCUS       DAYYHB010000180         2457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000180 DAYYHB010000000
VERSION     DAYYHB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.187"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHA6B_002782"
     CDS             132..344
                     /locus_tag="ACHA6B_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950301.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACHA6B_002783"
     CDS             506..1024
                     /locus_tag="ACHA6B_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8950302.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1154..1543
                     /locus_tag="ACHA6B_002784"
     CDS             1154..1543
                     /locus_tag="ACHA6B_002784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950303.1"
                     /translation="MKKDIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDE
                     IITRENIDLENEISKLESIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMR
                     QNIARFLTKLVSEWKSIISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="ACHA6B_002785"
     CDS             1814..2437
                     /locus_tag="ACHA6B_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8950304.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          946 a          310 c          409 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaagaa
//
LOCUS       DAYYHB010000181         2449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000181 DAYYHB010000000
VERSION     DAYYHB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.188"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..648)
                     /locus_tag="ACHA6B_002786"
     CDS             complement(34..648)
                     /locus_tag="ACHA6B_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8950305.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            886..1308
                     /locus_tag="ACHA6B_002787"
     CDS             886..1308
                     /locus_tag="ACHA6B_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950306.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1318..1521
                     /locus_tag="ACHA6B_002788"
     CDS             1318..1521
                     /locus_tag="ACHA6B_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950307.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1732..2316
                     /locus_tag="ACHA6B_002789"
     CDS             1732..2316
                     /locus_tag="ACHA6B_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8950308.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSDDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          807 a          386 c          437 g          819 t
ORIGIN      
        1 aagatgagaa gataataaaa gcagcatgat caactatttg tcattgacca tgctgtcttt
       61 ttttattcta tacacggttt gtctcgtaac attgtagtct ttagcaatct tagcaatcgc
      121 aacaccattt ttaagatctt caacgatgct tttgtaaaca aggcgacgtt ggggatcttt
      181 tgcgtctgca ctgtataatt tgggccgccc tttgtagacg ccgtttgctt ttgcgatctt
      241 aattccttgt gcctggcgac gtttcgattc agtccgttct tgttcagcga tcatggccaa
      301 aatttggatg atcagatcct taatgaattt atccaggaga ggattgccaa ttgcttcagc
      361 catgatcggt aaactagtaa tgatcagctt cacatttttc ttcttcaagt aattgactga
      421 agcgatgatc tcgtcataat ttcttccaag tcgatcaata gcttccacaa taaaaatatc
      481 ttgatctcgg acgtaatcta atgcttcttg gaaaacaggt cgatgagtaa tcgttgcgcc
      541 agattgtttc tcgatgaaaa ttctctctgc accgaatttt ttcatttctt caatctggcg
      601 ttgttcattt tgatcgatcg atgagacacg tatgtatgca actctcaaaa cttttaccta
      661 ctttctgcaa ctatttaaaa tacaccctaa tcatacacga ggaagccata tgtcactttt
      721 ctttttaaat ggcaatattt tttgtaaata aatgtcaatc aagaaaattt aaacgattat
      781 gacattaaaa atatatttta tgaattttct agtatataac ttgatttatc tgttaagatt
      841 tattaagaaa acgttttctt agttttgatt tcgaggtgat agacaatgaa aaataagttt
      901 attttatctt taggtatttt aactattgga attagtgtaa ctggaactac aattcctaca
      961 tttgctgata gtacaaatga accaactact gtagtagata ataaccaaga attaactcct
     1021 ttagataggg acggaatagc cattggtaac ccaactaacg acttaactgt tactgaaaat
     1081 tatttaatag tttctccatt acttgctcta gcagcttcta aaacgatacg agtgacagga
     1141 acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg gaattggaaa
     1201 gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta taaatcagct
     1261 ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg attttttatg
     1321 ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag taaaattaat
     1381 ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac acaaaaaatt
     1441 caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag agaccaaaaa
     1501 acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac attctttttt
     1561 tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt ttgctttatt
     1621 tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat gttcagttaa
     1681 actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt tttgaaaata
     1741 ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga cagcctaaat
     1801 aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag taatcgacca
     1861 ggattagaaa tggccattga ttttgttcgc tctgacgata cacttgtcgt ttggcgctta
     1921 gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact gaatgagcgt
     1981 ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag ttctacagga
     2041 cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt gatcctggaa
     2101 cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg tcgtcctgaa
     2161 aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg tacacctatt
     2221 aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta tttggcaaaa
     2281 atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa tgctgaaaat
     2341 ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag tgtctactga
     2401 taacttattc tcaaatgttg taaggatttt attcatttgt taatattaa
//
LOCUS       DAYYHB010000182         2406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000182 DAYYHB010000000
VERSION     DAYYHB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.189"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACHA6B_002790"
     CDS             complement(<1..103)
                     /locus_tag="ACHA6B_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8950309.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACHA6B_002791"
     CDS             complement(135..458)
                     /locus_tag="ACHA6B_002791"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8950310.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACHA6B_002792"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACHA6B_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DAYYHB010000183         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000183 DAYYHB010000000
VERSION     DAYYHB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.190"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHA6B_002793"
     CDS             316..570
                     /locus_tag="ACHA6B_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8950311.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHA6B_002794"
     CDS             571..888
                     /locus_tag="ACHA6B_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8950312.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHA6B_002795"
     CDS             1447..2382
                     /locus_tag="ACHA6B_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950313.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYHB010000184         2386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000184 DAYYHB010000000
VERSION     DAYYHB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.191"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            533..1564
                     /locus_tag="ACHA6B_002796"
     CDS             533..1564
                     /locus_tag="ACHA6B_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950314.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1673..2098
                     /locus_tag="ACHA6B_002797"
                     /pseudo
     CDS             1673..2098
                     /locus_tag="ACHA6B_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            2211..>2386
                     /locus_tag="ACHA6B_002798"
     CDS             2211..>2386
                     /locus_tag="ACHA6B_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8950315.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWG"
BASE COUNT          860 a          408 c          385 g          733 t
ORIGIN      
        1 tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc aatccatcaa
       61 taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc taccatagaa
      121 taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt aagaattatc
      181 ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg attctgaaaa
      241 aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga acgaaccaaa
      301 tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc aattaaaaga
      361 tcaaaagaat gactgcacta ataaaataac taaaaatgta tcttcttact tcatatcttt
      421 tagctagtac ttagttaaaa atttgcttcc taagatcaag acaaactgtc tatcttcatt
      481 tgatgaaatg cggtaaaata tagaaaatga acactattgg agggaatttg taatggacag
      541 caaaactaga aaacaactgc aagaaaagtt aaagcaacgt acacacttgc tcccttccgt
      601 ctttcaacag tttttagaag aaaatcgttc ttccctttca ttaggcagtc gctatgaata
      661 cgcaaaagat tttcatctgt ttactgatta tttgagaaaa gtattttctg aagaagtgga
      721 tgatcagctg attatccatt tagaaaaaca agattatcag aatttcttag ctaaaatcta
      781 tcgacatact cgttcgttcc aaactacagc gaaccaagaa acggagcaat tgtttgaaaa
      841 tagctattac gggatttcaa ggaagcaata ttcattaaat cattttttac gatttttgta
      901 tcaaaaagga ctgattgaag aagagatttc tcttttgggt acaagtttac cggaaacaac
      961 caaagcagct ccgcgatata tcgatgcaga acttttcaaa gattttgagc atttgtttat
     1021 cccgattgaa ggctttcaaa caagcagaga agctagcttt gaagaaaaga atagaccgag
     1081 gaaccttgca attactgcca ttcttctgta ttgcggatta aagatccatg aagtagtaga
     1141 acttaaacgt aaagatgtat ctttgaccaa gcagattctt aatctgaata gaaaagaaaa
     1201 cagactgaat aaagtaccaa taccaaaaga agcgatgcca tttctggaga actattttaa
     1261 gttaacagca caaaatacgg acgaaaattc ttttgttttt cgttcctcac atgatcagca
     1321 aatttctcct cggactattc gccaaatttt gagcaaaatt acagtatata aaaacgtatc
     1381 gccagaacta tgcagaaaaa catatcgtta ttatgcagaa ctatatttag atgatgctga
     1441 cgcggtaaat tatttatgtg gtaatcgata cctctcatcc cattcttttc aagatatctg
     1501 ggaaaagatg gttgattttt cttatcacga attagcggca ttcgttcggg tttcttccat
     1561 ataaaacaaa tgtatgatct ttttttcaca ataaactatg ttattatata aaggattatg
     1621 ttacgattat cttgaatttg tatattactt aacaaaagat aatggagtgt gaatgatgga
     1681 gaccataacg tattcccctt tgaacttatt tacgaatttt caagaagctg cagctaactt
     1741 ttccgagacc ccgattatct ttgatgagcc actttcagct ttcccagaat taggacttga
     1801 aacaacttac aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct
     1861 tggagtgaaa tccggagaca aagttattat ttataagagt tccgcttttg acacatacct
     1921 tctagctgtt tctgtgtctt accttggtgc agtgcctgtc atgacttctt atcatctacc
     1981 aacaacaaca atggaagtat ttatcgatcg tttagaagac cctttcattt tatttgatga
     2041 tgagacaaaa aagcgagtga gagaaatttc aaatggaaca aaatcaaaac agattcctaa
     2101 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
     2161 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
     2221 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
     2281 tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga aatactgatc
     2341 aactgaagca acgcgataca gtgattattg cttgtgttat atgggg
//
LOCUS       DAYYHB010000185         2354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000185 DAYYHB010000000
VERSION     DAYYHB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.192"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(118..189)
                     /locus_tag="ACHA6B_002799"
     tRNA            complement(118..189)
                     /locus_tag="ACHA6B_002799"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Glu,seq:ttc)
     gene            complement(201..274)
                     /locus_tag="ACHA6B_002800"
     tRNA            complement(201..274)
                     /locus_tag="ACHA6B_002800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Ile,seq:gat)
     gene            complement(287..357)
                     /locus_tag="ACHA6B_002801"
     tRNA            complement(287..357)
                     /locus_tag="ACHA6B_002801"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Gly,seq:tcc)
     gene            complement(362..434)
                     /locus_tag="ACHA6B_002802"
     tRNA            complement(362..434)
                     /locus_tag="ACHA6B_002802"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Phe,seq:gaa)
     gene            complement(438..510)
                     /locus_tag="ACHA6B_002803"
     tRNA            complement(438..510)
                     /locus_tag="ACHA6B_002803"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Asp,seq:gtc)
     gene            complement(529..602)
                     /locus_tag="ACHA6B_002804"
     tRNA            complement(529..602)
                     /locus_tag="ACHA6B_002804"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(566..568),aa:Met,seq:cat)
     gene            complement(615..704)
                     /locus_tag="ACHA6B_002805"
     tRNA            complement(615..704)
                     /locus_tag="ACHA6B_002805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(666..668),aa:Ser,seq:tga)
     gene            complement(727..798)
                     /locus_tag="ACHA6B_002806"
     tRNA            complement(727..798)
                     /locus_tag="ACHA6B_002806"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Glu,seq:ttc)
     gene            complement(820..907)
                     /locus_tag="ACHA6B_002807"
     tRNA            complement(820..907)
                     /locus_tag="ACHA6B_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(871..873),aa:Ser,seq:gct)
     gene            complement(932..1005)
                     /locus_tag="ACHA6B_002808"
     tRNA            complement(932..1005)
                     /locus_tag="ACHA6B_002808"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(969..971),aa:Ile,seq:gat)
     gene            complement(1019..1089)
                     /locus_tag="ACHA6B_002809"
     tRNA            complement(1019..1089)
                     /locus_tag="ACHA6B_002809"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Gly,seq:tcc)
     gene            complement(1091..1166)
                     /locus_tag="ACHA6B_002810"
     tRNA            complement(1091..1166)
                     /locus_tag="ACHA6B_002810"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Asp,seq:gtc)
     gene            complement(1185..1258)
                     /locus_tag="ACHA6B_002811"
     tRNA            complement(1185..1258)
                     /locus_tag="ACHA6B_002811"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1222..1224),aa:Met,seq:cat)
     gene            complement(1271..1360)
                     /locus_tag="ACHA6B_002812"
     tRNA            complement(1271..1360)
                     /locus_tag="ACHA6B_002812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Ser,seq:tga)
     gene            complement(1384..1457)
                     /locus_tag="ACHA6B_002813"
     tRNA            complement(1384..1457)
                     /locus_tag="ACHA6B_002813"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Ile,seq:cat)
     gene            complement(1471..1544)
                     /locus_tag="ACHA6B_002814"
     tRNA            complement(1471..1544)
                     /locus_tag="ACHA6B_002814"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1508..1510),aa:Met,seq:cat)
     gene            complement(1572..1644)
                     /locus_tag="ACHA6B_002815"
     tRNA            complement(1572..1644)
                     /locus_tag="ACHA6B_002815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1609..1611),aa:Ala,seq:tgc)
     gene            complement(1664..1737)
                     /locus_tag="ACHA6B_002816"
     tRNA            complement(1664..1737)
                     /locus_tag="ACHA6B_002816"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1701..1703),aa:Pro,seq:tgg)
     gene            complement(1747..1820)
                     /locus_tag="ACHA6B_002817"
     tRNA            complement(1747..1820)
                     /locus_tag="ACHA6B_002817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1784..1786),aa:Arg,seq:acg)
     gene            complement(1831..1916)
                     /locus_tag="ACHA6B_002818"
     tRNA            complement(1831..1916)
                     /locus_tag="ACHA6B_002818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1880..1882),aa:Leu,seq:taa)
     gene            complement(1933..2004)
                     /locus_tag="ACHA6B_002819"
     tRNA            complement(1933..2004)
                     /locus_tag="ACHA6B_002819"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1970..1972),aa:Gly,seq:gcc)
     gene            complement(2016..2088)
                     /locus_tag="ACHA6B_002820"
     tRNA            complement(2016..2088)
                     /locus_tag="ACHA6B_002820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2053..2055),aa:Thr,seq:tgt)
     gene            complement(2106..2187)
                     /locus_tag="ACHA6B_002821"
     tRNA            complement(2106..2187)
                     /locus_tag="ACHA6B_002821"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2151..2153),aa:Leu,seq:tag)
     gene            complement(2195..2267)
                     /locus_tag="ACHA6B_002822"
     tRNA            complement(2195..2267)
                     /locus_tag="ACHA6B_002822"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Lys,seq:ttt)
     gene            complement(2271..2343)
                     /locus_tag="ACHA6B_002823"
     tRNA            complement(2271..2343)
                     /locus_tag="ACHA6B_002823"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2308..2310),aa:Val,seq:tac)
BASE COUNT          647 a          646 c          546 g          515 t
ORIGIN      
        1 ttacattaga actgtccaaa aaagcttagc acaatcatcc ataaagataa ctctgctaag
       61 ctttttaaaa tcattctttc agataaaaag aaaaagatac tcaaaaagta tcttccatga
      121 cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
      181 caacgggcca ataaaataaa tgggcctaaa tggactcgaa ccatcgacct cacgcttatc
      241 aggcgtgcgc tctaaccagc tgagctatag gcccataaaa actaaaagcg ggtgacgaga
      301 atcgaactcg cgacaacagc ttggaaggct gtggttttac cactaaacta cacccgcata
      361 atggcgcggg acagaatcga actgccgaca catggagctt caatccattg ctctaccaac
      421 tgagctaccg agccaaacgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg
      481 caggcatgtt aacccctaca ccacggaacc aatgttttta gtttttaatt gcgggagcag
      541 gatttgaacc tacgaccttc gggttatgag cccgacgagc taccagactg ctccatcccg
      601 cgataatatg aaaaaggagg ataagggatt cgaacccttg cacggtttta cccgcctgac
      661 ggttttcaag accgttccct tcagccggac ttgggtaatc ctccatgtaa aactatatta
      721 aattaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa
      781 ccgcttgacc aacgggccag gtaaaacaaa atattgaaac ggagaaggag ggatttgaac
      841 cctcgcgccg gtttcccgac ctacaccctt agcaggggcg cctcttcagc cacttgagta
      901 cttccccaaa accaataaga ttttgtacta atgggcctaa atggactcga accatcgacc
      961 tcacgcttat caggcgtgcg ctctaaccag ctgagctata ggcccataaa aaactaaaag
     1021 cgggtgacga gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac
     1081 tacacccgca tggcggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
     1141 catgttaacc cctacaccac ggaaccagtg tttttagttt ttaattgcgg gagcaggatt
     1201 tgaacctacg accttcgggt tatgagcccg acgagctacc agactgctcc atcccgcgat
     1261 aatatgaaaa aggaggataa gggattcgaa cccttgcacg gttttacccg cctgacggtt
     1321 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc atatgaaact tacaacgcca
     1381 caatggacct tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctaacc
     1441 aactgagcta aaggtccagg ctcttaaaaa tcgcggcgga ggggatcgaa cccccgacct
     1501 cccgggtatg aaccggacgc tctagccagc tgagctacac cgcgaaaaag atattcatta
     1561 tttaaatgaa atggagccta gcgggatcga accgctgacc tcctgcgtgc aaagcaggcg
     1621 ctctcccagc tgagctaagg ccccgtgttt aaatttataa ttatcgggaa gacaggattc
     1681 gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta cttcccgttt
     1741 aattaacgcg cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta
     1801 tccagttgag ctatgggcgc atatttgaaa tgccgaggac cggaatcgaa ccggtacggt
     1861 gatcactcac cgcaggattt taagtcctgt gcgtctgcca gttccgccac cccggcgcga
     1921 ttgctttggc aaagcggaaa acggggttcg aacccgcgac ccccaccttg gcaaggtggt
     1981 gctctaccac tgagctattt ccgcgtgttt aaagatgccg gctaaaggac ttgaaccctc
     2041 gaccctctga ttacaaatca gatgctctac caactgagct aagccggcat gtaaaactta
     2101 aattatgcgg gtgaagggac ttgaaccccc acgccgtaag gcgctagatc ctaaatctag
     2161 tgcgtctgcc aattccgcca cacccgcaaa aatatgagcc gtacagggct cgaacctgtg
     2221 accctctgat taaaagtcag atgctctacc aactgagcta acggctcata tggaggttaa
     2281 cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc
     2341 tccatggata actt
//
LOCUS       DAYYHB010000186         2324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000186 DAYYHB010000000
VERSION     DAYYHB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.193"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..558)
                     /locus_tag="ACHA6B_002824"
     CDS             complement(112..558)
                     /locus_tag="ACHA6B_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8950316.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(585..761)
                     /gene="rpsU"
                     /locus_tag="ACHA6B_002825"
     CDS             complement(585..761)
                     /gene="rpsU"
                     /locus_tag="ACHA6B_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8950317.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(923..1351)
                     /locus_tag="ACHA6B_002826"
     CDS             complement(923..1351)
                     /locus_tag="ACHA6B_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8950318.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1443..2303
                     /locus_tag="ACHA6B_002827"
     CDS             1443..2303
                     /locus_tag="ACHA6B_002827"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8950319.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          693 a          461 c          376 g          794 t
ORIGIN      
        1 aaaaacagct agaagaaaga ataagattct ttcttctagc tgttttttag ttcttttttt
       61 acgaacacaa aaaactgcgg acaatcactg tccgcagttt aaatgataaa attaagattg
      121 taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt tgactttagg
      181 cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag tttgagtgat
      241 tgcttcttga acaagttgtc gaatttcttc atcggaaagt tgttccggca tatatttttc
      301 gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg cttcttcaaa
      361 ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta gttcttcttc
      421 ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact tgatcattcg
      481 gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt catcgtttaa
      541 tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa ttttttgcgt
      601 tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta gaattcacgt
      661 ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg acgaagagca
      721 tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc ctccctccga
      781 gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga ctgtcctgta
      841 aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt taaaattatc
      901 aaatgttatt ttatttaaac ccttattgac aattttcaca tcttccgaaa atctcgaatc
      961 tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc ggacacatat
     1021 ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg ccgatttccc
     1081 cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct tccagtaaat
     1141 cgatctctgc aaaatcatgg agattccgat aaatcgtatc ataacttacc cctttgaatt
     1201 cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc ttcactaaat
     1261 aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc aatgtttcta
     1321 atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca aaatgattac
     1381 gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt gaggttttat
     1441 ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac tctgctggag
     1501 aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt gagttcaacc
     1561 tattccgcag aacgttcgtg acagatgaaa aaacattaaa gcaggcattg caagatgcat
     1621 tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc gagataacac
     1681 gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca ccgatatcag
     1741 aaattgaacg tttaaccggt atcaagccaa ttcggcaacc tggtgccctc catttattga
     1801 atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac gatgatggga
     1861 aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc cgaacttcca
     1921 aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat ttacctttga
     1981 tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa atcgtgggac
     2041 taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc tcttacggat
     2101 taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactc gattttgcga
     2161 atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta tctattgaag
     2221 aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat tatggaactg
     2281 aaacaacaga aaaaagcgat taatcatttt tgtctttttc ttaa
//
LOCUS       DAYYHB010000187         2296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000187 DAYYHB010000000
VERSION     DAYYHB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.194"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACHA6B_002828"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACHA6B_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACHA6B_002829"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACHA6B_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACHA6B_002830"
     CDS             2093..2254
                     /locus_tag="ACHA6B_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950320.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          810 a          373 c          405 g          708 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tatttt
//
LOCUS       DAYYHB010000188         2293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000188 DAYYHB010000000
VERSION     DAYYHB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.195"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1740
                     /locus_tag="ACHA6B_002831"
     CDS             127..1740
                     /locus_tag="ACHA6B_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8950321.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT          718 a          407 c          446 g          722 t
ORIGIN      
        1 gttttatcca caaattattt ctctatattt tctgtctatt caaaataaac aataattttc
       61 cggcacaatt atttgctgtt ttttaccata acatagtacg atcaacgtag aaaaggagat
      121 gtttttatga aagttgttgt cattggcggt gtagctggcg gtccttcatt tgccactcgt
      181 ttccgtcgat tgaatgaagc gcacgaaatc attatctatg aacgcggaga aaatatttct
      241 tatgcaagtt gtgctttgcc ttattattta ggaggtgtga tcactgaccg tgactcgctg
      301 atcgaacgta caccagaaat attgaaaaca aaaaacaaca tcgatgtctt tactaaacac
      361 gaagtaacag caatcgatcc ttctactaag cgattaacag ttaaagacct atccacaaat
      421 gaagaaataa aaacagatta cgataagttg atcatctctt ctggtgctag accagattat
      481 ccggatattc ccggagtttt tgaagcagaa aacggctttg tcctccgtag tgtgacggat
      541 gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac aacatgtcgt cattcttggt
      601 gcaggtgtta tgggtctgga attagctgag aatttcaagc atcgcggctt agacgtgact
      661 ttaatcgatc aattgccaca agtcgctttc ccttatgatc cagaaattgc taatttagtt
      721 tatgacaaat tgctgaaaga aggattagcc gttcatttag aaacaagagt tactgagatc
      781 cgtgataaag gtcgagaaat agtattatca gatggctccg tcctttctgc tgatatgcta
      841 atttttgctg ttggtgtttc tccgaagaat gaagtggtga aagcaaccgg catacaatta
      901 tcagatacag gtcagatcat tgttgatgac cagttacaaa ccaatctgcc ggacatctat
      961 gcgattggcg atattatcga aacgactagt gtagtgactg gtcagccgat ccaaagtatg
     1021 ctttccagtg cggccaatcg tcaaggacat atgctggcag atattttaaa tggtacgcct
     1081 atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa tctttgatca tacagcaagt
     1141 tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca taacaaatta taaaactgtt
     1201 tttatcactc cctttgacca tgcctatttc tatccagaag ctacaagatt aaatctaaag
     1261 ctgatttttg atgcagatag cggtcgtatt ttaggtggac aagcatttgg agaaaaaggt
     1321 gtcgataaac ggatgggaga actttctgta gcaatcaccg gaaacttgac agtctttgat
     1381 ttgcctgatt tggagttgcc ttactctcca ccgtattcta ctacccgtga cccgttgaat
     1441 atagctggtt atgtcgcaat caatcaaatg acgaatatcg tagaaacgat caaagcaagt
     1501 gatatacccg aaaacgattt gaaagaagcg ttctttttag atatacgtga acctaataaa
     1561 gcaccttctg gcagtatctc ggcaacaaaa aatattccaa tgaatgagtt acgagatcga
     1621 atcaatgaaa tcccaaaaga taaaaaagtt tacattactt tcagaagagg attgaataat
     1681 tatacttctg cccgaatctt ggcaggtttg ggtatcaaag cgattttgat tgaagaataa
     1741 aaaaacgacc gcggtgggta gccgcggtct aggagttatt tttacttgag gagtaaaaat
     1801 gaaaaagtat atagggttgt tggttggtat gtatatagaa tagaagataa atgtgaagaa
     1861 attgtgcatt atttcttcac atttattagg ttatagttta agaaagttta aacattgcgc
     1921 aatttaatta ttttagctgt ttcttttcat aaaaaaacat gttgtgactc tgagacaaat
     1981 catagtccat agtgatgtct ctagagtaac tacgcctaat attacgaggc atcatattaa
     2041 tctatcagca ctagtaccta aactagcatc cctttcgaat attttttctt atataataaa
     2101 gtttgctttc actcctctaa tctcaccaaa tcgactggct tgcgttgtgt gatactagga
     2161 actgattggt ttagaacgac ttttctattg taatcatatt tttcgccatc ctcgttgtct
     2221 tctaatcctt caatgaatga tccctaatgt gtgtattaaa taatttaatt tttttgttcc
     2281 tatttattta aca
//
LOCUS       DAYYHB010000189         2274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000189 DAYYHB010000000
VERSION     DAYYHB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.196"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..>2274)
                     /locus_tag="ACHA6B_002832"
     CDS             complement(718..>2274)
                     /locus_tag="ACHA6B_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8950322.1"
                     /translation="DQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDNAYTKFDFRN
                     GRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPDFPRIA
                     ITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFK
                     QFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIV
                     TFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDE
                     HTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEEQIGVYETVK
                     GSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKAL
                     AKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSE
                     KELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMKRAWRKMLDD
                     ELIPLENELR"
BASE COUNT          658 a          450 c          356 g          810 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatc
//
LOCUS       DAYYHB010000190         2264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000190 DAYYHB010000000
VERSION     DAYYHB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.197"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            723..1055
                     /gene="traE"
                     /locus_tag="ACHA6B_002833"
     CDS             723..1055
                     /gene="traE"
                     /locus_tag="ACHA6B_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8950323.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1671..2012
                     /locus_tag="ACHA6B_002834"
     CDS             1671..2012
                     /locus_tag="ACHA6B_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8950324.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          791 a          298 c          465 g          710 t
ORIGIN      
        1 tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt
       61 cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata
      121 ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc
      181 taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg
      241 tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc acttttggat
      301 gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg
      361 gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt
      421 cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa gtgataccac
      481 tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca
      541 taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg
      601 aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta
      661 tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt
      721 ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta
      781 ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt
      841 gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat tacattggga
      901 aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca
      961 aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt
     1021 gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt
     1081 ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact
     1141 agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg
     1201 ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt
     1261 ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta
     1321 gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga
     1381 cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg
     1441 tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag
     1501 catttaccgc tgtctagggt ttctcgtttc ggacgccata aacgagtcga aattttttat
     1561 gtatttttaa gtaaacgaaa aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa
     1621 ttaatataaa gcatagagaa aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt
     1681 atgatccgaa tatacgattg ggcacacata ctattaaggt ttctttccaa cgttgggatt
     1741 acaaaggatt tgtgacgttt agaaggggcg gaaattgtaa aggattagat gttttagcat
     1801 tagacgagga tgatttatac gatcaaacat tgactgataa tccaattgga ttcggcttgc
     1861 tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac gcttatgaat gataatggag
     1921 acgaattatc tgttgaagat acttggtcat atttgagtga ttatattgtc agctttgaaa
     1981 tcattgaatt tgtagcggat aaagaagaat aaaaactgga ggaataaatt ttacagataa
     2041 aggcttagaa ggagtcacga cgattaataa aaaggtagta ttaccttagg cattgagtaa
     2101 ggaatttatt tagtctaatg gtgataagtc aagatgaaat ttaagatttt ctttattgag
     2161 taatatgttt tgaagcgcac gaaactagac ccgatcaaaa tcaaattttt ttcgggttga
     2221 ttcaaggatt tgcgtcaata acggattgtt atttctctat caca
//
LOCUS       DAYYHB010000191         2243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000191 DAYYHB010000000
VERSION     DAYYHB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.198"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..>2243)
                     /locus_tag="ACHA6B_002835"
     CDS             complement(144..>2243)
                     /locus_tag="ACHA6B_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8950325.1"
                     /translation="RKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGYLQI
                     VNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKDLL
                     EIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSAMV
                     NKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASELPGV
                     STGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEKQY
                     EDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNYQT
                     LINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGSVV
                     KAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSNTY
                     MIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGISR
                     SVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHLVK
                     AIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPLAS
                     AKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHINL
                     TIAKEIMDAYYDMFMAK"
BASE COUNT          571 a          504 c          411 g          757 t
ORIGIN      
        1 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
       61 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
      121 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
      181 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
      241 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
      301 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
      361 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
      421 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
      481 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
      541 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
      601 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
      661 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
      721 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
      781 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
      841 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
      901 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
      961 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
     1021 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
     1081 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
     1141 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
     1201 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
     1261 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
     1321 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
     1381 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
     1441 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
     1501 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
     1561 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
     1621 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
     1681 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
     1741 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
     1801 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
     1861 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
     1921 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
     1981 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
     2041 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
     2101 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
     2161 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
     2221 ttaggttctt gtgcaccttt tct
//
LOCUS       DAYYHB010000192         2106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000192 DAYYHB010000000
VERSION     DAYYHB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.199"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..1550
                     /locus_tag="ACHA6B_002836"
     CDS             84..1550
                     /locus_tag="ACHA6B_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8950326.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          641 a          369 c          435 g          661 t
ORIGIN      
        1 aaataacact gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat
       61 ttttatcata aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt
      121 taattgcatt taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag
      181 gagtatcagt tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat
      241 tgatcgttgg acaattaggg tcagccttca aggatagtaa aggaggcgtc agttcatggg
      301 tcgaaaatac gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg
      361 tccatattcc ttatttggca caaaagcctc aagcaatcct gattgctggc ggttgggcag
      421 tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac
      481 ttattatatt tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga
      541 ttggcggact ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg
      601 taacggcacc gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga
      661 aaacatatat tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg
      721 cagttggagg agcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg
      781 aatttcctaa agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct
      841 cagtcgcaat gggtatgctg tttgctagcg ataatattcc taaagacttg atggcaaatg
      901 gggcatacag tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga
      961 tcatttacgc attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg
     1021 caccactaaa aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta
     1081 aacgtaatcg taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa
     1141 tcattatttt gttgccgatc tttggtatca aagatatgaa tgaattagta aaatggttga
     1201 cgaacttgaa ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga
     1261 tgttgaataa agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa
     1321 tcggttttat ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa
     1381 tggtgccaaa agtagattat actgctgatc ctaaagcatg gtggttccaa ttattcacga
     1441 atatcatcac accaattgtc ttgatcttat taggtatgat cttgccagca attgctcgtc
     1501 gagacaaaaa caaagaaatc actgaaccag aaacagtggt tcagccttaa aatagaaata
     1561 cttgttacaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga
     1621 aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt tcgcagaagc tacttctgga
     1681 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
     1741 caaaaatagc ccctcatatt tttcgttttg ctgggcttat taccgcagat gcaagctttt
     1801 gaacaccgtt taagaaagag gtagtgagcc tgccgtttaa ctccgagtca caaggagcaa
     1861 tttcaaaaaa caacttctca tgtttgttga ctattgtgcc ttgtgaccga ggtgttggca
     1921 ggagaacacc gtttaaggaa gaggttgtaa aatcagcgat ttgacctgag tattagagga
     1981 acaaacgaaa aaatagctcc tcatattttg ccgttttttg agttaatgct gaaggtcagc
     2041 tgattgaaca ccatttattc gaaacaaggg actttgacac gacttttgtc acagtccctt
     2101 gttttt
//
LOCUS       DAYYHB010000193         2078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000193 DAYYHB010000000
VERSION     DAYYHB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.200"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(397..1011)
                     /locus_tag="ACHA6B_002837"
     CDS             complement(397..1011)
                     /locus_tag="ACHA6B_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8950327.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1617..1994)
                     /locus_tag="ACHA6B_002838"
     CDS             complement(1617..1994)
                     /locus_tag="ACHA6B_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950328.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          685 a          408 c          316 g          669 t
ORIGIN      
        1 caattttgtt atatcaggta aacttcattg caataatgat aacaaatgta ctataattac
       61 tttgtaaaga gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat
      121 ccatatggat tctttcttgt ctctttttcc actacttttt atttttctga cactacatat
      181 tcttataact catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa
      241 aacaagctta aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt
      301 tgttaatcct cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca
      361 ttattttttt aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac
      421 tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc
      481 tgttccgcag atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc
      541 gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga
      601 tttgatactt cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt
      661 gatccactga gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa
      721 aatgtcttct gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt
      781 ttcatagaca cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg
      841 atcccaaaat tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc
      901 accttgatta atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat
      961 ccttaatttc tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc
     1021 ccgcctagct atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa
     1081 aaacagtagt tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt
     1141 gaacgaatac gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat
     1201 atctacatag gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact
     1261 gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat
     1321 tcttagcgcc aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt
     1381 attgtaagta atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat
     1441 ttatatatat ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc
     1501 tttaaaaaag attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct
     1561 ctcatctcaa tagtttatcc attattttat ctttttttgc gtatacctac taaatattac
     1621 cgtcttcttc tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta
     1681 tgtctacctt ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga
     1741 tacagtacag caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca
     1801 accatcaagg tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct
     1861 ttttttctga ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat
     1921 atccaattgc ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg
     1981 actaattttc ccatagtgaa cacacacctt tttttataat taaaaaattc gcttttttat
     2041 tctactatga atcttctggc ttggctttca tttctctt
//
LOCUS       DAYYHB010000194         2069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000194 DAYYHB010000000
VERSION     DAYYHB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.201"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2069
                     /gene="acm"
                     /locus_tag="ACHA6B_002839"
     CDS             <1..>2069
                     /gene="acm"
                     /locus_tag="ACHA6B_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8950329.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     LIVTICA"
BASE COUNT          808 a          341 c          408 g          512 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg atagtaacta tctgtgctg
//
LOCUS       DAYYHB010000195         2069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000195 DAYYHB010000000
VERSION     DAYYHB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.202"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..581)
                     /locus_tag="ACHA6B_002840"
     CDS             complement(<1..581)
                     /locus_tag="ACHA6B_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8950330.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFL"
     gene            complement(582..1490)
                     /locus_tag="ACHA6B_002841"
     CDS             complement(582..1490)
                     /locus_tag="ACHA6B_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8950331.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVERVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1490..>2069)
                     /locus_tag="ACHA6B_002842"
     CDS             complement(1490..>2069)
                     /locus_tag="ACHA6B_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8950332.1"
                     /translation="HNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIE
                     LPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRN
                     LTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIK
                     EFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          691 a          358 c          235 g          785 t
ORIGIN      
        1 tttagaaatt caggtttaaa agaatacatt gaggcattca tttcaaaaac ttcaggtgtc
       61 tgttgcctag atgtgtatct tgatttatta actatagtaa cttcattttc aattcttttt
      121 accatattaa aatagggatt acgtcttgca gaaacaactg aaaagtagac gtctttatct
      181 aaatttttta gatgttcatc tattaattct tcaagattag aaacttcacg taaaggtgaa
      241 gtcaaatcta aatctataat taaatcaaat atattatctt ctctttctag ttgtaagaat
      301 gaatcacgta tcacttcaat tttccctaca gtatctgtag ctaattcagg tttacgttct
      361 acaattttca cagacggatt taacttagtc gtcaattcta ctaactcatt actgtccgta
      421 ttcaaacaag tcgtgcaatt atgttcagga tgtttttgtt gatagatatc tatcattgct
      481 aaagcgaaat ggactagggg ctttcctgcc aaatatttca aatttttatt tttaaacccc
      541 ttagacccag ctctaccaca aatagtaaat aaaatttcca tttatttctc ctcagctatt
      601 ttcaatactt cgtttgcatg atataaatca ttccagtttt ctttgcctgt tactacgttt
      661 tcaaagaaat atttcaactc cttctcatac atatcattag cagattcagg catttcttta
      721 acaatgtttt cttcaaaact tattttatta tttaataagt caacattgat acattcatct
      781 aaagtaaaca attgaaattt tcttgtagga actcttccaa aataatctaa atgaatttct
      841 ataattttat catcgtatct caatatatat gttgctaaat cattactgtt aatttcaaga
      901 ttcgaatatt taccaaactg tttttttact gatttaggga aaccaaatag gtttataata
      961 taatccaatt catgtataca gtctaattct actcctcccc ccatagattt aaaggcagaa
     1021 taattatttc tataatcagt accttttctc caatcaggaa gataagtaga acaaatagct
     1081 cttacagaat atactttttt actttgaata ctattacgaa caacttccat gatattttta
     1141 tacctcagtg gagcagcaac ataaatattt ttgtaatcat aattatattg agtaagatca
     1201 atactctcag aaaaagcagg tttttctaaa aatatgtaat caactctttc aacaattaca
     1261 tttagtgttt ccagatgctt aaaagtcgga tttgtaataa aagctatatc ataatgtgta
     1321 tccaaatcgt ctctattttg aatatacgtt atttcttcaa actctttttt tccactgcta
     1381 ctataagctg ttatatcaaa attaatagaa ttcttttttc catagcttat tagatttttt
     1441 agatgtctct ttccaattga ccctaaccca aaaaaaatta ctttcatcat taatttacct
     1501 caatgtcgtt aattaagtta ataatatact tatctttttc aaaagtatat tggggttcca
     1561 ttttcttttc caaaagcgtg aaaaattctt taatctcctc taaatatgca tcttcaataa
     1621 tagtatgtga ataattatta tcatgcataa cctcatcgta caattgaata tctgtcatac
     1681 tagaagtttc ttcattccat gaaaatagag aatccgggtt accttcccat tctattaaaa
     1741 tcttttcacc aattatagtt aaatttcttt tagctactct tgaaacaaca tctacactta
     1801 aagttccaac tataccagtt tcatgctcaa taataagagt gaaactgtcc gaataatcaa
     1861 tatcaagatt ggataatttc tgggtcatta cagtaaacga tttaactttc ccaaatgtat
     1921 ctataatcca aggtaattca atagcgaata tttctctaca cccattagtt tttttgtttg
     1981 aaacaaaaaa attgcgataa tcttcccaag gatgccagtc gggtaaatac tgtcctacat
     2041 ggtattgata agaaattttt ttattatgc
//
LOCUS       DAYYHB010000196         1992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000196 DAYYHB010000000
VERSION     DAYYHB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.204"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHA6B_002843"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHA6B_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8950333.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHA6B_002844"
     CDS             852..1040
                     /locus_tag="ACHA6B_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8950334.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHA6B_002845"
     CDS             1053..1610
                     /locus_tag="ACHA6B_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8950335.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHA6B_002846"
     CDS             1642..1884
                     /locus_tag="ACHA6B_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8950336.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DAYYHB010000197         1975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000197 DAYYHB010000000
VERSION     DAYYHB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.205"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1285
                     /locus_tag="ACHA6B_002847"
     CDS             <1..1285
                     /locus_tag="ACHA6B_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8950337.1"
                     /translation="KFSVNMLNTKAKDPTDAIFGIKPDGTIAHFCISEMPHMLVAGTT
                     GSGKSVLLNEILVTVMCHSTPDELKIGIVDPKRVEFGRYKKLPFMLADPITDMDEAYD
                     FFEYLVILMHERYKLMEKAGLQNITLYNEYAEKNGLDRFPYVILLVDEYSQLVGTHKE
                     VEGLIVQLGQMARAAGIHVILATQSPRSTVVTGIIKANFPSRVCLMVASDLESRIVLD
                     EGGGESLSPKGDMLIKLVDGSMVRAQGAYISNKEIETIFNHLRNTMPEPEYVDYRAIV
                     AETRGEGGEDEDFGGIGKAPVRKSAKRNLNTPLANPSSKEPSIEKKSAFAIKLEKNKE
                     EKVEQGAKTLEKLQAEKLEKVLRALGKADPEPQTSNNTNNKQPIASKTKPKANDPKGS
                     ENVVITTDKSEKKQINGSTSPIMQKMLKAKKQVHK"
     gene            1282..1911
                     /locus_tag="ACHA6B_002848"
     CDS             1282..1911
                     /locus_tag="ACHA6B_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950338.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
BASE COUNT          750 a          305 c          421 g          499 t
ORIGIN      
        1 aaaattcagt gtcaatatgt tgaacacaaa ggctaaagac ccaacagatg ctatttttgg
       61 aattaagcct gatggcacaa ttgctcattt ttgcatcagc gaaatgccac acatgttggt
      121 tgcaggaacg acaggatcag ggaaatcagt attactgaat gaaattcttg ttacagtaat
      181 gtgtcactca acgcctgatg aattaaaaat aggaattgta gacccaaaaa gggttgaatt
      241 tggaagatac aaaaaattgc cgttcatgtt ggctgatcca attacagata tggatgaagc
      301 ttatgatttt tttgaatatc ttgtgatctt gatgcatgaa cgttacaaac tgatggaaaa
      361 agcagggtta caaaacatca cgctatataa cgaatatgcc gaaaaaaatg gattagatag
      421 atttccctat gtgatcttat tagttgatga atattcacag ttggttggaa cacataaaga
      481 agtagaaggt ttgattgttc aattaggaca aatggcaagg gcagcaggta ttcatgttat
      541 cttagcaaca caatctcctc gatcaacagt tgtcactggt attatcaaag ccaattttcc
      601 gtcacgtgtt tgccttatgg tagcaagtga tttagaaagt aggattgtat tagatgaagg
      661 cggtggagaa agtctaagtc caaagggaga tatgcttatt aagcttgttg atggaagtat
      721 ggttagagcg cagggagctt acatcagtaa caaagaaatt gaaacaatct tcaatcacct
      781 tagaaatact atgccagaac cagaatatgt tgattaccga gcaatcgtag ctgaaactcg
      841 tggtgagggc ggtgaagacg aggattttgg tggaattggt aaagctcctg taagaaaatc
      901 tgctaaaaga aatttgaaca cacctttagc aaatccatca tctaaagagc catcaattga
      961 aaagaagagt gcgtttgcta ttaagcttga aaagaacaaa gaagaaaaag tcgagcaggg
     1021 agctaaaaca ctagaaaaac tgcaagcaga aaaactagaa aaagttttaa gggcattagg
     1081 aaaagctgat cctgaaccgc aaacctcaaa caacacgaat aataaacagc cgattgcatc
     1141 taaaaccaaa cctaaagcta atgatccaaa aggctcagaa aacgtagtga tcacaactga
     1201 taaatctgaa aagaaacaaa taaatggaag tacttcacct atcatgcaaa aaatgttaaa
     1261 agcaaaaaaa caggtgcata aatgatcatc gtcagttctt ttttaagatg gcttactcca
     1321 tataaatata cagatgaagt agttgtgaaa cgagcgaaga aaagagataa aaaagcggta
     1381 ctaactccta aacagatgct tgatcatggc attttagggg tgaaggtttt agaagtgaat
     1441 aaaccgtttg aatcaaatag tgcaagagaa aagttgcttg gagctgaagc cgatgtcgca
     1501 tatttttacc atgacaaaag caagcatcaa atagctatta aattcaatcc aaaatcggca
     1561 tggcggtatc aagcaagaga tgacttgaaa cgtaggaaac ttagaccaat gctctatcca
     1621 gaaggaaaag aagcatttga tagaacccac atcgttccca ttggttttca tggtacagaa
     1681 tcagataacc gcctattagt aggttggaac agcaatcaaa atagagttca gatcaataaa
     1741 tttgagaaga aagtaaggaa attcaacgat cgaaaaacga tcctatggtt tgcagatatt
     1801 tgtaaacagc cggatggaac agcaaagtgg gaagcggtga tatttgacga aaatggtaaa
     1861 aaagtaatgt ctgaaacatg gcatgataaa gctaaatttg tttggcagta gcagtagagg
     1921 tgaaaaagat tgctagaact ttttgaagca aatagaacag gctttaatca aaaat
//
LOCUS       DAYYHB010000198         1971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000198 DAYYHB010000000
VERSION     DAYYHB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.206"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..392
                     /locus_tag="ACHA6B_002849"
                     /pseudo
     CDS             43..392
                     /locus_tag="ACHA6B_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(664..954)
                     /locus_tag="ACHA6B_002850"
     CDS             complement(664..954)
                     /locus_tag="ACHA6B_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950339.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1162..1854)
                     /locus_tag="ACHA6B_002851"
     CDS             complement(1162..1854)
                     /locus_tag="ACHA6B_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8950340.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          547 a          373 c          297 g          754 t
ORIGIN      
        1 attacttaaa gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt
       61 gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt
      121 tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga
      181 catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa
      241 tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt
      301 caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg
      361 aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc tctttttatg
      421 gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt
      481 taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata
      541 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
      601 atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt
      661 tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata
      721 ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct
      781 attattataa taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt
      841 caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct
      901 ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt
      961 cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc
     1021 tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga
     1081 aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt
     1141 aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt
     1201 atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa
     1261 gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc
     1321 tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt
     1381 ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag
     1441 atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg
     1501 tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc
     1561 tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc
     1621 tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat
     1681 cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc
     1741 aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc
     1801 aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat
     1861 agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag
     1921 cgtttttaaa ttacatatgt aattttaata taaatactat caaatactca t
//
LOCUS       DAYYHB010000199         1933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000199 DAYYHB010000000
VERSION     DAYYHB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.208"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHA6B_002852"
     CDS             <1..435
                     /locus_tag="ACHA6B_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8950341.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHA6B_002853"
     tRNA            complement(759..842)
                     /locus_tag="ACHA6B_002853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHA6B_002854"
     CDS             1370..1870
                     /locus_tag="ACHA6B_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8950342.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          626 a          364 c          329 g          614 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat tta
//
LOCUS       DAYYHB010000200         1910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000200 DAYYHB010000000
VERSION     DAYYHB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.209"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1377
                     /locus_tag="ACHA6B_002855"
     CDS             <1..1377
                     /locus_tag="ACHA6B_002855"
                     /inference="COORDINATES: protein motif:HMM:NF037025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8950343.1"
                     /translation="LLKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDA
                     QFNCTTEGSGSGTDSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNW
                     KQESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGG
                     AWWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAV
                     RGGNAKEIWKYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTD
                     DANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIY
                     QGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLW
                     AVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVG
                     KAKFEIEK"
     gene            1400..>1910
                     /locus_tag="ACHA6B_002856"
     CDS             1400..>1910
                     /locus_tag="ACHA6B_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950344.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGA"
BASE COUNT          662 a          265 c          467 g          516 t
ORIGIN      
        1 ttattgaaaa aatctaagag aaagattgcc acgttgctta tgttaatggt gcaatttaca
       61 gggattgcaa tgagtgtaat cacaataaat ccgcaagtga tacatgctga tgggatagct
      121 acttatgatg cacaatttaa ctgtactact gaaggtagtg ggtcaggaac agatagcgat
      181 ggaaacgata ctggaagtgg cgctgtatca ggtgatgtag caaaaaatat tgatgatatt
      241 tacaaattta tgaatgggga atatgggcta tcaggtgcta ctatcgcagg tattctaggt
      301 aactggaaac aagaatcagg taatgatcct cgaacggtag aaggttatta cggtgatgga
      361 cagagagaag ttgacttcat caaacaattc actgatggtc tgatgggtat tggctatggt
      421 caatggacag caggtcgcca tactgcatta gtaaactggg cgaagaaaaa ctttggtggc
      481 gcttggtgga cgacagaagc gcaattggat tttatgttca aaggtgatgg tggatttgtt
      541 gctatattaa aagattatgc tttaaacgct tctgatgatg ttgtggcaaa tgccgtagcg
      601 tttcataaga attgggaaat ttcagcagat tccgaatcag ctgtagtggc tgtacgtggt
      661 ggaaatgcaa aagaaatctg gaagtacatg aaaagcaaag gcatggacgg agctaaagat
      721 gtaagcaaaa tcaataaaat tgccggagga acaagtaccg gtggtggatc aaataattca
      781 tcaagctcag catcaggaac agtagaagat gcctgtgcaa ctacagatga tgctaattct
      841 ggttctggat caatgggtaa agaaggaaaa attaacggta aaaaaggtca agtaatagcg
      901 aatatgactt atgaagaagc tttgaaaaaa tatgggaaag cgatcacgct accaaaattc
      961 aaagaaccag attggtcaac ttcacctttt aacggaatat atcaaggtca gtgtacagag
     1021 ttgacgtggg cttatatgag ccaattatac gatgggaatc aaccgactac tggaaatggt
     1081 ggctacgtat gggaaagcta caaatcggca ggagcgaaaa taacgaacaa accaatcgta
     1141 ggttatgggt ttagtgcttc agatggatgg ttatgggctg ttggcgatgc agggcatact
     1201 ggtgtagtta tagcggtgtt tgaagatggg tcatttctat gtgctaactt taatgttcca
     1261 ccaacccaag ctccaacaag gggagtttcg gtaactttga ttgatggagt ggacggatca
     1321 gataatgtgc atttctttag cggtgtagga aaagctaaat ttgaaatcga aaaataaatt
     1381 ggaatgaagg acgatataga tgaatagtaa agtgaaagat acaattatca tttctttgct
     1441 ttctggtatg ttattagcac tgcttgttgg aatcctattc ttcaataagc agaatcagga
     1501 catagaagct gaaaaacaca gtgtgcatat tgaagataag ctgacattct ttaaagaggt
     1561 taaaccaaat gaggtggatg gattacggaa agatttagaa aatgatattc aagaaatgtt
     1621 gaatggatca gttcaaaata agtttgcaga tcaatcaggt gaagaatttt tgagaaaact
     1681 atttattgtc acaggtaaac caccaattac tgataagagt agtgagaaag aaaaaatcga
     1741 gtactataag ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg
     1801 gttgtatgaa gcattgaccg attttgaagt tcaatacaat ggaaaaaaag tgtatgatac
     1861 cacattaacc atcacactaa ataatgataa gaaagtgact gggggtgctt
//
LOCUS       DAYYHB010000201         1898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000201 DAYYHB010000000
VERSION     DAYYHB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.210"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1161)
                     /locus_tag="ACHA6B_002857"
     CDS             complement(<1..1161)
                     /locus_tag="ACHA6B_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8950345.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIA"
     gene            complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="ACHA6B_002858"
     CDS             complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="ACHA6B_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8950346.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(1606..1800)
                     /locus_tag="ACHA6B_002859"
     CDS             complement(1606..1800)
                     /locus_tag="ACHA6B_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950347.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          525 a          392 c          343 g          638 t
ORIGIN      
        1 agcaataaat aatggacgca gttcttgttg ccgacagctt aatcgcttct caggagaaaa
       61 gtctttccat ttcttttcta ggacaaacca tttatccaat tgtttgactg cttgagctgc
      121 gggaatatcc gtgtttttct ttccttttgg aattgcttcg aaaaatttcc gccgaacatg
      181 cgcgaaacaa tagacaagtc gtacactttc cacggcatta tacccgctat agccgtcaca
      241 gtgtaaatac cctgtaaact ccttgagaaa agttttcgga acatctccag cacgactttc
      301 gtcgtggtga taataaacga tgggatgctg actgtctttt cctgtacaaa actgccagta
      361 ataggtatct gttttggcac tttcaatcac acgataactt gtttcatccc cgtgtagaat
      421 ctcttcttga ttaagataga ctcttaatcg ttctcctaaa gggtctaacg cgttttgcac
      481 tgctttaata tgccaattgg tgatattatc tctcgaaata tccaacccat gtgtttgttt
      541 ccaatagttc tcttgccgat aggcaggaac tttctgacca aatttcatcc ggatcgtttc
      601 cgccactaaa ctattggaac ctaagctatt agaaatcaaa ggtttaggaa cgatcgcttt
      661 tttaaaagaa gaaagtcctt ctttttcgca gcttttacaa caataggtat gttgataatg
      721 ggtgagacaa tccaacatag cctgatggaa acgaatttct tctcgtactt ttgtccgtcc
      781 gatatctttc atctgtgaac cgcaatgttc acaaaattgt tcttccaaag gaaggagatg
      841 ttctttctct gttataggta agtgtttgat tttgtctgct ttcgttccta caactcgttg
      901 atgtcctttt actggaataa tggtggcagc tgcttctgga acacttacag aaatttcttc
      961 atcactaaaa agattcaatt gcccattcgt ttcctcaaag agagtctctt tagagcgtcc
     1021 aaataatttg ttggttaaat aattgacctt ttcattcaat agttggattt cagttgtaag
     1081 attctgaatg gtttgttgtt gtgcttgatt ggttttatgg gcttcttgaa gcaactgaag
     1141 caacgtatct gtagtttcca aaatttttca ccaccttgaa tcgaactgat tttgttacag
     1201 aataccacaa gaaacgcaca agaaataggg gttcaacaca ttttttgtgt taacccccca
     1261 gcttaaaaaa ctcctttttt tcctggcaaa atagctcgag gttgatcgat acttaatcct
     1321 tctaacaacc atcgaacctg ttggttcgtt aaggttcgaa cttcgtcttt cgtccgaggc
     1381 cattttaact tgccgttttc tatccgctta taacagagaa gaaaaccatc accgtcccaa
     1441 tagagaagtt tgaaacggtc ttgacggttc ccgcaaaata aaaaaacggc atcttcgtaa
     1501 agctcgagtt gatactcttg ttgaataaga ctggcaagac catcaattcc ttttcgcaaa
     1561 tctgtttttc cgcacacaat ataaatatgt ttggtttgaa gtaaatcata aagcattgga
     1621 caacagttct ttcaaaatag cttgaagaat ataattgtta ataccgttat agatcaaaac
     1681 ttcagaacca ttctctcttt tgatgcgcat cgctaacttg gcagccgcgg gtttaccttt
     1741 ttctggtgca ctcttttcgt ttcctaattg gacaggaaca atctcattta atttatacat
     1801 agaagacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
     1861 tactgttcta tggggcgctt acgttaaaaa tgttttgc
//
LOCUS       DAYYHB010000202         1858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000202 DAYYHB010000000
VERSION     DAYYHB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.211"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1624
                     /locus_tag="ACHA6B_002860"
     CDS             32..1624
                     /locus_tag="ACHA6B_002860"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8950348.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            1617..>1858
                     /locus_tag="ACHA6B_002861"
     CDS             1617..>1858
                     /locus_tag="ACHA6B_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8950349.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNEL"
BASE COUNT          648 a          300 c          392 g          518 t
ORIGIN      
        1 aactttaaat aagagatagg agtgtatctt tttgggtgca gaattaaatc aaaaattatt
       61 tagtgcggca gataatctcc gtagcaaaat ggatgcgtca gaatataaaa attatttatt
      121 gggactgatt ttctataaat atttatccga ccgtttgttg gaacaagtgg ttcttttagc
      181 agatgaatcc ttagaggaat atgatacggt aagcaaacaa acgatgttgt atcgcgaatt
      241 actttcagat gaagaatcaa aagaagatct gattgcaaca atcgttgata ttttggggta
      301 tgcgatttcg ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt
      361 ccaattgaat gatctaaaca aagcctttgt gcaattggca tctacctaca atcaatttaa
      421 tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg agcaacaacg
      481 aaatgtgacg attaccgaag tcattaagaa attgaatgac gtggaagttt taggtcacga
      541 tggggacgtg atcggggatg cttatgaatt tctgatcagt caatttgctt cagaagcagg
      601 gaagaaagcc ggagaatttt acacgcctca tatggtttct gatatgatgg cacaaatcgt
      661 gacgttggat caaaaagaac gacgattctt tagtgttttt gatccaacca tgggttcagg
      721 ttcattgatg ttaaatgtgc gtaactactt aactcaccca gataacgtta aatatcatgg
      781 gcaagaatta aatacaacga catacaatct agcaaaaatg aacttgattt tacatggggt
      841 agatgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggatt ggccaacaga
      901 tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta attggtcagc
      961 agatacgacc ttcttagacg attctcggtt taatcgttat ggaaaattag cgccaaaatc
     1021 caaagcagat tttgcgtttc ttcttcatgg tttctatcat ttgaaggaaa cagggactat
     1081 ggccatcgtc ttgccacacg gagttttatt ccgtggcgct gcagaagggg tcattcgtca
     1141 aaaattacta gaagacggca gtatttatgc tgtgatcggt atgcctgcga acttgttctt
     1201 cgggacatcg attccaacga cggtgattgt attgaaaaag aaccgccaaa ctcgagacgt
     1261 attattcatt gacgctagtc gagaatttgt caaaggaaaa aatcagaata aattatcgga
     1321 agaaaacatt caaaaaatcc tcgaaaccta tgctgaaaga aaagatgtcg agaaatacgc
     1381 tcatttggca aactttgatg aaatcaaaga aaatgactat aacttaaata ttccacgcta
     1441 tgtagatacg tttgaagaag aagaaccaat tgatatggtt catgtcggaa atgacatcaa
     1501 gaagatacga caagaacagc aagtgctaga aaaagaactt ttagaagcta tctcttccct
     1561 gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt tcaaacatga
     1621 gtaatgatat acaaccagaa attcgtttcc caggatttac ggaagcttgg gaacagcgta
     1681 agtttgaatg cttacttgat aaaaaagatg gagtaaggag gggaccattt gggagtgcct
     1741 taaagaaaga attctttgta tctaatagca attttgttgt ctatgaacaa caaaatgcta
     1801 tttatgataa ttacgaaaca agatataaaa ttactgaaaa aaaatataat gaattaat
//
LOCUS       DAYYHB010000203         1838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000203 DAYYHB010000000
VERSION     DAYYHB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.212"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1449
                     /locus_tag="ACHA6B_002862"
     CDS             10..1449
                     /locus_tag="ACHA6B_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM8950350.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1579..>1838)
                     /locus_tag="ACHA6B_002863"
     CDS             complement(1579..>1838)
                     /locus_tag="ACHA6B_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950351.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          741 a          162 c          318 g          617 t
ORIGIN      
        1 gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat agatgatgat
       61 tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta tgatggtaga
      121 gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg ggaagatgaa
      181 gtttttagag taattattga atataacaat gttcctattg gatatggaca aatatataaa
      241 atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat agtctatggt
      301 atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac aagatatatt
      361 aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat tttagaccct
      421 cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag aattattgaa
      481 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
      541 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
      601 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
      661 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
      721 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
      781 cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta taaagaaatt
      841 aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga acaaaatttg
      901 ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta tacagatatt
      961 agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt gttgcgtgaa
     1021 actatttata atgatttaac tgatatagaa aaagattata tagaaagttt tatggaaaga
     1081 ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga ttttagttgt
     1141 aatcatctat tgttagatgg caataataga ttaactggaa taattgattt tggagattct
     1201 ggaattatag atgaatattg tgattttata tacttacttg aagatagtga agaagaaata
     1261 ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat tgagaaagca
     1321 aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt ttatggaatt
     1381 aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa aaggacttat
     1441 aaagattgat tatataatat atgaaaagct attataaaag acattagtat taaatagttt
     1501 aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt aaaatgctat
     1561 acaatgtttt taccatttct acttatcaaa attgatgtat tttcttgaag aataaatcca
     1621 ttcatcatgt aggtccataa gaacggctcc aattaagcga ttggctgatg tttgattggg
     1681 gaagatgcga ataatctttt ctcttctgcg tacttcttga ttcagtcgtt caattagatt
     1741 ggtactcttt agtcgattgt gggaatttcc ttgtacggta tattgaaagg cgtcttcgaa
     1801 tccatcatcc aatgatgcgc aagcttttga atattttg
//
LOCUS       DAYYHB010000204         1824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000204 DAYYHB010000000
VERSION     DAYYHB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.213"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..535
                     /locus_tag="ACHA6B_002864"
     CDS             17..535
                     /locus_tag="ACHA6B_002864"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8950352.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="ACHA6B_002865"
     CDS             663..1346
                     /locus_tag="ACHA6B_002865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8950353.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="ACHA6B_002866"
     tRNA            1655..1728
                     /locus_tag="ACHA6B_002866"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
BASE COUNT          605 a          292 c          372 g          555 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acat
//
LOCUS       DAYYHB010000205         1800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000205 DAYYHB010000000
VERSION     DAYYHB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.214"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1484
                     /locus_tag="ACHA6B_002867"
     CDS             <1..1484
                     /locus_tag="ACHA6B_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8950354.1"
                     /translation="DGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDA
                     DKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIE
                     VTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGT
                     TYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTIS
                     VVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPK
                     LEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGK
                     SQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1618..>1800
                     /locus_tag="ACHA6B_002868"
     CDS             1618..>1800
                     /locus_tag="ACHA6B_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8950355.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFY"
BASE COUNT          709 a          267 c          388 g          436 t
ORIGIN      
        1 cagatggtac gaaagataca gtgaaagttc cagtagaagt gacagacaat cgttccgatg
       61 ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga agttgggggc acagtagatc
      121 taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta
      181 ctcccggcgg tacaattgat accaacacac caggtaatta cgaaggtgtc attgaagtaa
      241 cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt
      301 ctgacgccga taaatatgaa ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag
      361 tagatttaac agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag
      421 atgttactcc cggcggtaca attgatacca acacaccagg caattatgaa ggtgtcattg
      481 aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca
      541 atcgttctaa tgcagataaa tacaatcctg aaggacaaaa agtgactaca gacttaaata
      601 aagagccaga tgcatctgag ggaattaaga acaaagaaga tttaccaaaa ggaactactt
      661 atgcttggaa agagaaagta gatgttagca cagctggaaa taaaaaaggt actattgtag
      721 tgacatatcc agatggatca tctgatgaag ttgaagtaga cgttacagtg acagatactc
      781 gttccgatgc agataaatat aatcctgaag gacaaaaagt gactacagac ttaaataaag
      841 agccagatgc atctgaggga attaagaaca aagaagatct accaaaagga actacttata
      901 cttggaaaga aaaagtagat gttagtacag ctggaaacaa aaaaggtact gtagtagtaa
      961 catatccaga tggatctaaa gaagaagtag aagttactat ttctgtagta gataaaaaag
     1021 caccgaataa acctcaagtt gatcctatta cagagggtga ccaaattgtt actggtaaaa
     1081 ctgaaccaaa tgcagaggtg acagttacat taccagacgg aagtcaacac catgggacag
     1141 ctgataaaaa tggtaatttt acagttaaag ttcctaaatt agaggcaggg acaaaagtga
     1201 tagtaactgc aactgatgaa tctggtaata ctagtgaacc tactaatgta gttgtttctt
     1261 ccaacaaaaa agacagtgga aaaaacggta gtaaaggtag taagactgac aatcaaggta
     1321 gcaacagcaa tcaagacaaa aatagaggca aaagccagtc ctcaaaagtt tttcctaaga
     1381 cgggagagag tgacagtaat atctttacta ttagcggtgg tttaatactg ttaggaacgt
     1441 taggattatt agggtataaa aatcgaaaaa aagagaatga atagaaaatc aatttcttaa
     1501 gtcttctctg agaagaggaa gaatcttttt cttcctcttt ttgctttttt atgatgggaa
     1561 ataagtgtta gttaaagatg aatatacttt ttatagaaag gaaaagaatg taattaagtg
     1621 gaaaaaatag taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac
     1681 ttaagcgatt caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg
     1741 gaatgtggga catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat
//
LOCUS       DAYYHB010000206         1767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000206 DAYYHB010000000
VERSION     DAYYHB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.215"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..338
                     /locus_tag="ACHA6B_002869"
     CDS             141..338
                     /locus_tag="ACHA6B_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950356.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            444..926
                     /locus_tag="ACHA6B_002870"
     CDS             444..926
                     /locus_tag="ACHA6B_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950357.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(929..1525)
                     /locus_tag="ACHA6B_002871"
     CDS             complement(929..1525)
                     /locus_tag="ACHA6B_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8950358.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          549 a          266 c          355 g          597 t
ORIGIN      
        1 ttaattgtga aaacgaaaac agtacgctat aatgcctgta aggttgattt ttaactgtaa
       61 ataaaaatca atgagttaaa tctccaaaga aatagcagtg gtaatgtgga gaaaagttcc
      121 agcagtttag gaggattttg atgacggaag aaatgattaa tttaggagaa caatatgcat
      181 gcaaaccaat tggttttaca aaaacggtca ttggtgaagt tgtatcaaaa atgacaaact
      241 gcgccgtggt caaagttgcc caatgtgctg cagaggacca agaattatta gatgaaaaag
      301 caagcatggt tgttgcaaaa tatgatacat ttgaataaag aaaacaattg gacgtgcggc
      361 gtgctgcaag tccaatttct ttgagaaggt aaatcagtat tcttttccta ttttctggag
      421 aagaaaaagc gaggcgagtt gtcatggtaa cattctttac ggttctattg tttttattcg
      481 ttgttgtgat gtttttattt ctttggctgg ttcgaaaaga aattatttat cggactgtgc
      541 gaaatcgatg ggtctacttg gtgataccct tcttagtgtc actagtcatt tggtatacac
      601 tcatcagtca tcctacagca gatgaattgg caaaagggat cttgtcagca atgattttta
      661 ttagtttttt gttggacagt agaggaatca cagaagaagg acttgttttg aatagctttg
      721 acaaaaaagg tgttcctttt tctgagatca ataaaattgt attatatcag ccaaaaggtt
      781 ctaaaatcgt aaaaatgaat tttttccgaa acggatggcg aggaccgatg ctgaaatttt
      841 ctgcatcatt agaagaatta gttccatttt tgtcccaacg cttaaacgat gaggcagaga
      901 ttgatattat gattgatcca gaataaaatt atccaaatac gcggaagcga tcggcatcgt
      961 tcatgtattc tttttcccca gccggtgttt ctggtgaacc gaatactaat tgcgaacgta
     1021 gtttccagtt tgaagggata ttccattctt ttgcaactgc ttcgtcaata actgggttgt
     1081 aatgttgcaa gtttgcgccc aatcctttat caactaatgc tacccaagta ttagctgttg
     1141 cgattccgtt tgattgttct gaccaatctg ggaagttgtc tgcatataat tcaaattgtt
     1201 cttgtagacc ttttaccacg tcagtatctt tgaagaataa tactgtacca taaccatttt
     1261 tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc tgctggagtc aatgggcgaa
     1321 gtgcttcttc agtgatttcc caaagttttt catgtgcatc tccgaaaagg ataactgcgc
     1381 gtgttgattg agcattgaaa gcagttgggc tttctttaat tgcttctttg atcaaagttg
     1441 ttaattcttc attagaaagt gaaacattac gtcctagatg atagattgaa cggcggtttt
     1501 tcaaagtgtc agtaaaagtt gtcataataa aatttctcct ttgatttgcg ttttgcattt
     1561 ttttaattga ttacgaaatg aagtatagca ccttacaaaa agtaagtaaa cagatatgct
     1621 cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag aaaaatcgtt atgtaaggat
     1681 tttcttagtt atcgctttca ttgttgtgtt ttaggaattt ttgcttattt gaaaaaaaca
     1741 gctgatatat aattattaaa tctctat
//
LOCUS       DAYYHB010000207         1756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000207 DAYYHB010000000
VERSION     DAYYHB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.216"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1756
                     /locus_tag="ACHA6B_002872"
                     /pseudo
     CDS             1..1756
                     /locus_tag="ACHA6B_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          680 a          285 c          392 g          399 t
ORIGIN      
        1 aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc aatcattgac
       61 agcggacgag tgacgcaatg gaaaaaaatc cgaccattta ttttctgaaa ggctatcgaa
      121 aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa taccaagcca
      181 cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta ccaactgatt
      241 ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa aatgatccag
      301 aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc gatcaactca
      361 aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat tttgaagaag
      421 tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc gtcaacagta
      481 atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag aaggaacaaa
      541 aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa gaaacaaaaa
      601 caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa gttaaatccc
      661 tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa gacgtcaaag
      721 aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgattg
      781 aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa aaagaaggaa
      841 tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg gtcaaaggat
      901 tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa gataaaaaac
      961 gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt gaaaaagtgt
     1021 ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt ccgaaccgtg
     1081 tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg attcaagcgg
     1141 tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc tatcaaagag
     1201 aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa gaagaaatcg
     1261 tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat gaattagccc
     1321 atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg caggcagaaa
     1381 tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa gctattcctt
     1441 atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct gcggaacgtg
     1501 ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc atttctaatg
     1561 aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg aagcaggagc
     1621 ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt tcactttgga
     1681 tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta catttgaagg
     1741 atgcggcgtt tattga
//
LOCUS       DAYYHB010000208         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000208 DAYYHB010000000
VERSION     DAYYHB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.217"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA6B_002873"
     CDS             131..505
                     /locus_tag="ACHA6B_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8950359.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA6B_002874"
     CDS             complement(619..1248)
                     /locus_tag="ACHA6B_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8950360.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLSQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA6B_002875"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA6B_002875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          241 c          283 g          556 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgactcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYHB010000209         1610 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000209 DAYYHB010000000
VERSION     DAYYHB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.218"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>1610
                     /locus_tag="ACHA6B_002876"
     CDS             26..>1610
                     /locus_tag="ACHA6B_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950361.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYR"
BASE COUNT          508 a          255 c          224 g          623 t
ORIGIN      
        1 tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat
       61 ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta agggaatcta
      121 tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac tagataatgt
      181 atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga tgaatatttt
      241 ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg gaaatcttgt
      301 tttaagttat aatatctact atttttttat cactttttta acaatggtcg tttcttattt
      361 ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga ttatttatac
      421 attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag aagctgtcgc
      481 tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta gagattacca
      541 aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt tactttctgt
      601 cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt tttgggacca
      661 aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc
      721 gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag tcccactggg
      781 aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa ataataacta
      841 taacaactat actattggat tattcttatt tctcggattg attggtgctt ttatttttat
      901 aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg ttcttttttg
      961 ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt
     1021 tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat
     1081 ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc
     1141 actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac
     1201 acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc
     1261 aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa
     1321 ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa
     1381 ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc
     1441 ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac
     1501 tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc
     1561 ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
//
LOCUS       DAYYHB010000210         1598 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000210 DAYYHB010000000
VERSION     DAYYHB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.219"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1318
                     /locus_tag="ACHA6B_002877"
     CDS             149..1318
                     /locus_tag="ACHA6B_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8950362.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          573 a          257 c          259 g          509 t
ORIGIN      
        1 atccatagtt aatttatcat aatatcccat attttaaaat aaaagatagc gtaaaatatt
       61 ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca
      121 cacagaaatt ttataggagg actttctcat gactaaattt actatagaaa ttatgcaaac
      181 actactcaat aaaggggatt tagatgaatt atttcaggat catttagagc gagcgattaa
      241 ttcacttcta caagcagagc taactgcttt tttagattac gaaaaatatg atcgaaatgg
      301 attcaattca ggaaattccc gtaacgggaa ttattcacgt acatttaaaa cagaatatgg
      361 cgagttgaat ttaactattc ctagagatcg gaatggcgaa ttttctcaac aaacactacc
      421 agcatataag cgaaccaatg attcattaga aacgaccatt atccaattat ttcaaaaagg
      481 cattactatg gctgaaatct ccaaattaat tgaaaaaatg tatggtcacc actatacgcc
      541 acaaacaatc tccaacatga gtaaattggt ggctgaagat gttttagctt ttaaagaaag
      601 aacgttagaa gccaattatt ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg
      661 acagactgtt tcgaaggaag cagtgtatat tacgattggt attcgtttgg atggaaccaa
      721 agaagttctt gggtttacta ttgccccaac tgagtctgct tatatttgga aagaagttct
      781 tcaagatctt agaaaacgtg ggttagaaga agttttatta gtagtgacag acggattaag
      841 ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc caatttcaac aatgttgtgt
      901 gcatgtatct agaaatatcg ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga
      961 ggatttcaaa ttggtttacc aagcgaattc aaaagaagag gcattggatc acatcgactt
     1021 tatgactagg aaatggaaaa agcagtatcc aagagtcgtc aatttactct tgaatcctgc
     1081 cctattaacc ttttataatt tccctcacgc catcagacga acaatttatt cgacgaacct
     1141 gattgaaggc tttaataagc agctaaaacg atatactcga agaaaagaac aattccctaa
     1201 tgaagaatct ctagagagat tcttcatttc tcaatttaat caatataacc aaaaattttt
     1261 aggtagaatt cacaagggat ttaaagaaat tcaggataca ttagagtcga tgatttaact
     1321 gtaattaaca gaatggattt tccatttaca tataattctt gacgctacca taatctaaat
     1381 aaccatcata ttcatctact acttatataa ttgagtttta ttaaactttt taagtttcta
     1441 ttctatcaaa tatttaaaca atctctgcat ccgaaaagac tacctcgtat agttttacta
     1501 acaatatttt tattagcgtg ttcaaatcct gatttcttgt atcaattatt ttctgttcac
     1561 tttcaataag aaaaaagtct ttacttccat ggataata
//
LOCUS       DAYYHB010000211         1531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000211 DAYYHB010000000
VERSION     DAYYHB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.220"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACHA6B_002878"
     rRNA            <1..1342
                     /locus_tag="ACHA6B_002878"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACHA6B_002879"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="ACHA6B_002879"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAYYHB010000212         1519 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000212 DAYYHB010000000
VERSION     DAYYHB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.221"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1519)
                     /locus_tag="ACHA6B_002880"
     CDS             complement(<1..>1519)
                     /locus_tag="ACHA6B_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950363.1"
                     /translation="KIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSLESIFS
                     SPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYV
                     MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKK
                     ESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTL
                     LLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLI
                     CLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFST
                     LFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNE
                     QMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKG
                     KTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKIS"
BASE COUNT          590 a          207 c          203 g          519 t
ORIGIN      
        1 atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt attccacttt
       61 cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt tttgaattaa
      121 cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg ccttttgaag
      181 atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gctactataa ataacttgac
      241 catcttctgt aacttgatgg tttttcaaaa gcaattgatc ttttttatag cctataggta
      301 cataatcaaa attctcaaat tcttcaagtt tattttccat ttgttcatta gaataagttc
      361 cttgaaaatt catttctttc accatgttaa ctactgatga ataatttaaa attgaaatag
      421 ctaatattaa taatagtata aaatttcttc tccttgtata agaaaatttt tccaactgtg
      481 gagaaataaa tagagatact gaaaaagata tgaacaatgt tgaaaatatc ataaaacgcc
      541 aaggagcttg aataatattt aaaggagtat tttgtaaggc aaaccatgga aatatttttg
      601 tacaaagtag aatagatatt atacccatta tataagtgat tttagataag attcctaatt
      661 ttttaaagtt aaatgttagt ataattaaac aaataagcaa agttaagcca ataccaaatg
      721 atagtaattc tgaatctata cttccaatca aaaactcttt acccagataa attgctcttt
      781 tttgtaatat atccacataa ggtctattca gatcttgata caatgtctgc tcaatcatag
      841 gacctataaa cgctagtgaa agaagtaaag taactatagc agcttttatt aatttaataa
      901 ttaagaagat ttctattttc tttttccgat aaaattgaat aattaaagca attataatca
      961 ttaccatagt catagctaca gaaagaacat gagtgtaaat taataaagtc atgctaatcg
     1021 ccaacataac ccaagattct ttttttgagt cgtaaagctt tataaacccg agtaaaataa
     1081 gaggaagtat tgacattgcg agtaattccc ccattgcccc tcgaagaaat atttctacac
     1141 tcctaaaaga agatgttgta tatataatag cgaaaaagga gctagtaaca tgttttttgg
     1201 taatttcata cattacataa tgacatataa atagagtaat gagcgtaaca atataaagaa
     1261 aaaggttata gcctattgct aaattagtaa acttgatcaa taaaaataaa ggatatagag
     1321 ttaaccatgg gtaaaaatta tttacttgca tgccatatga tctaaacgtt tcaaaattaa
     1381 taggggagct aaaaatagat tctaaactta acgcacgatt aatgtgaaat gccgtatcat
     1441 aaaagccaaa tatttccatg tttttaaatg ctggaaataa atataataaa ctaacaaatg
     1501 cataaactat aaatatttt
//
LOCUS       DAYYHB010000213         1515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000213 DAYYHB010000000
VERSION     DAYYHB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.222"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1025
                     /locus_tag="ACHA6B_002881"
     CDS             <1..1025
                     /locus_tag="ACHA6B_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950364.1"
                     /translation="KSNIIRKDYDMAKQAYLDAELKKLEQRYDAEHLPEVKQQEDEYR
                     KATEESLDQQYQEAIESIKNDALKQSQEAKEVVVERIINSLKPEIEKSLTDVQEKTLE
                     IVEKTKSENEAEFHDTKEKTTDVVVKYFANKEEVEKQVANNTKELETKLVEQQKTIEE
                     YKDRMKEVKDLAEGYKLNSEKLAEQSEALREDLSRSRSDLAQMAEKTKSLAVATENQS
                     SSKRDFTSKKMATIISVAAMACGTILGSSYFISHNNQQAQAQTQTSISRVENSIGTNT
                     NSASTNSSYEATPTTEFKVGKDVVISIDGKTTKAEVISIDGEKAVVKTPDGNQYKIQA
                     HLSDNN"
     gene            1037..1270
                     /locus_tag="ACHA6B_002882"
     CDS             1037..1270
                     /locus_tag="ACHA6B_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8950365.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            1260..>1515
                     /locus_tag="ACHA6B_002883"
     CDS             1260..>1515
                     /locus_tag="ACHA6B_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950366.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILI"
BASE COUNT          603 a          231 c          290 g          391 t
ORIGIN      
        1 aaaaatccaa tatcattcgt aaagattatg atatggcgaa acaggcttat ttggatgctg
       61 aactaaagaa actagagcaa cggtatgatg ctgagcattt accagaagtt aaacaacaag
      121 aagatgaata tagaaaagca actgaagaaa gtcttgatca acagtaccaa gaagcaattg
      181 aatctattaa aaacgatgca ttgaaacaaa gccaagaagc aaaagaagtt gtggtggaac
      241 gaattatcaa ttcattgaaa ccagaaattg aaaaatcatt aacagacgtg caagaaaaaa
      301 ctttagagat tgtcgaaaaa acaaagtcag aaaatgaagc tgagttccat gatacaaaag
      361 agaaaacaac agatgtggtt gtcaaatatt ttgcgaacaa agaagaagtt gaaaagcagg
      421 tagctaacaa cacgaaagaa ttagaaacaa aacttgtaga gcaacaaaaa actatcgaag
      481 aatataaaga tcgaatgaaa gaggtcaaag atttagcaga gggttataaa ttaaatagcg
      541 aaaagttggc agaacaaagt gaagctttaa gagaagactt atctcgttcc cgaagtgatc
      601 tagctcaaat ggcagaaaaa acgaaatcat tagcagttgc tacagaaaat caatcaagtt
      661 caaaacgtga ttttacaagt aaaaagatgg ctactatcat ttctgtcgct gcaatggctt
      721 gcggaacgat cttgggatcg tcctacttta ttagtcataa taatcaacaa gcacaggctc
      781 aaactcaaac atctatttcg agagtggaaa attcgattgg gacaaatacg aactctgctt
      841 caacaaatag tagctatgaa gcaacaccaa ctacagagtt caaagttggg aaagatgttg
      901 tcatcagtat tgatgggaaa acaactaagg ctgaagtgat ttctattgat ggagaaaaag
      961 cagttgtgaa aactccagac ggaaatcagt acaaaattca agctcactta tctgataata
     1021 actaaggagg gctattatgc gagaagaaaa aatagtttat ccagtaattc aaaacaatca
     1081 agaaaacatg acctcttctc agcacttaca ggaagcacag ctcgtgtatc aggtaaatga
     1141 actaccacaa acgaacgaga caatgagcaa gtcaatatct ttaattggct tgcttattgt
     1201 tgcaatagtg ataactgtag gtgtttaccg taagttgaac gtgaaaggaa gtatttctga
     1261 tggcagataa ctcaaactat ggatttaaac ataacttaca tcaaaaacct tctgaaacaa
     1321 ttgtcagagt tgaatggctt gaaaagcatg tcaatagcac ctattctgtt acagcaaaaa
     1381 gattaattgt atcagctata gttgttgcag tcgttgttct tggatcaatt gtttacttag
     1441 tttctgatac gattaaactg aaatttattt ttgttccaac gttattaatg ataattggat
     1501 ttcttattct aatta
//
LOCUS       DAYYHB010000214         1515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000214 DAYYHB010000000
VERSION     DAYYHB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.223"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..694
                     /locus_tag="ACHA6B_002884"
     CDS             287..694
                     /locus_tag="ACHA6B_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950367.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ACHA6B_002885"
     CDS             708..1109
                     /locus_tag="ACHA6B_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950368.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ACHA6B_002886"
     CDS             1111..1482
                     /locus_tag="ACHA6B_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950369.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DAYYHB010000215         1513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000215 DAYYHB010000000
VERSION     DAYYHB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.224"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1458
                     /locus_tag="ACHA6B_002887"
     CDS             289..1458
                     /locus_tag="ACHA6B_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8950370.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          550 a          217 c          246 g          500 t
ORIGIN      
        1 taatatacta ccatttgttg gtagtataat ttaaaggtaa taagttattc ttttattact
       61 atgtttgggt tttatagcaa agaaagttta ataagagttt ggacctagca aacaatatta
      121 atgaggtcat taaatcttat tatctttagt gatataacga aatatctaca cagacaaaat
      181 gatagcgtaa aatattttgt gtaaatagat atttagggta gaaaaaaaga aagtccattc
      241 tgtaaaatta aagttaccac aaccgaaatt ttataggagg actttctcat gcccaatttt
      301 actacagaaa ttatggaaac actaatcaat aaaggtgatt tagatgaatt atttcgatgt
      361 catttagaat tagctgttaa ttcattattg caggctgaat taacagcttt tcttgattat
      421 gaaaaatatg atcgtgctgg gttcaattca ggtaattccc gtaatgggaa ttattcacgt
      481 tcattcagaa cagagtatgg agaattaaat ttggtaattc ctagagatag aaatggtaag
      541 ttttctcaac aaactttacc tgcttataaa agaaccaatg actctttaga aaccactatt
      601 attcagctat ttcaaaaagg aattactatg tctgaaatct ctgaattgat tgaaaaaatg
      661 tacgggcatc actatacgcc acaaaccatt tcaaatatga ctaaaatagt ttctgaagat
      721 attattgcct ttaaagaaag gtccttagaa tccagatact ctgtcatttt tatggatgct
      781 actcacattc ctttaaaaag acaaactgtt tcaaaagaag cagtttatat tgtaattggc
      841 attcgattag atggaaccaa agaggttctt gggtttagtc ttgctccaac tgaatctgct
      901 tatgtttgga aagagattct tcaagaccta aaagaccgtg gtttaaaaga ggttttatta
      961 gtcgtaactg atggtttaag tggcattaac gatagtattc atagtgttta tccaaatgct
     1021 caatttcaac aatgttgtgt ccatatatct agaaatatcg ctcataaggt tcgtgttagt
     1081 gatcgacaag aaatctgtag cgatttcaaa ttagtttatc aagcaagttc aaaagaagaa
     1141 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
     1201 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
     1261 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
     1321 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
     1381 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
     1441 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt
     1501 gacgctccca gtg
//
LOCUS       DAYYHB010000216         1509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000216 DAYYHB010000000
VERSION     DAYYHB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.225"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..923)
                     /gene="thiD"
                     /locus_tag="ACHA6B_002888"
     CDS             complement(108..923)
                     /gene="thiD"
                     /locus_tag="ACHA6B_002888"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8950371.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1217..1492
                     /locus_tag="ACHA6B_002889"
     CDS             1217..1492
                     /locus_tag="ACHA6B_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950372.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          461 a          307 c          234 g          507 t
ORIGIN      
        1 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaactt
       61 gttccttgtg actcggagct ggacggcagg ctcacgacct ctttctttta ctctaattca
      121 ccagctgccc aatgattgat gggtccatat ttgtgcccta catttatttc ttcacgaatt
      181 gcacggttta catagtcttt tgccaatcgg atcgcttgtt caactgtttt tcctttcgct
      241 aactcagctg caatacatgc agatagcgaa tcgccagttc cgttgatccg gtcggtagga
      301 taaaatggtg catttaacca aaatgatttt cctgattcga gcaatacgta gtcattgact
      361 gtttcttgct gcgcatcttt agcatgatgt cctttgatca taacgttttt tgcacccata
      421 tcttgcagtt cttttgctgc ttgcacaaaa tcttcttcag ctgctaatgt ccgtccagtc
      481 agcttttctg cttcataaaa attaggtgtc agtacttcgg ccaaagggac aagcttttct
      541 ttcaagcttt gcaaagcaga ttcttctagt aaaagatttc catgtttcgt tacgattacg
      601 ggatccacga ctaatggtcc aaaatcatat ttttggtaat tttttacaac tgtctcaatc
      661 aactttgaat ctgctagcat acctgtttta gctgcacgta tttgaaagtc cttcgcaatc
      721 agttcaaatt ctttgtcgat aaaatctgtt ggcaatgtca cacttgctcc gattccgtaa
      781 gaattacctg caacacatgc ggtcatgaca gaaacaccgt aaacatttct tgtaaagaat
      841 gtatgcatat ctgcttgcat ccctgcgctt ccatcgctat ctgatcctgc gatcgtcatt
      901 gcttgtacat actgatcttc catcaaatcg attcctccat ttttataaaa agatttttca
      961 taatgctgtt actgtttcta tcatatgttt ttcaaagtat ccctcagtaa atataccact
     1021 accttttctt ggtaatcaaa tttatttgaa aaaacatttg taataaatca gaaaatagtt
     1081 gtcatttgtt tgtattcgct attctaataa taaataacca ctatttgatt tatatatatt
     1141 tcagtatcct tcttgttttc atataaccaa aggcgctatg atttccatat gaaaaaaata
     1201 aagtcaggtg aaaaaaatga aacttgagga taaaaaagtc attttgaaga aaatcaaacg
     1261 actaccagac caaatagatg gctatacgat ctgttctttc aaaaaagaca ggagtataaa
     1321 agcttatacg caaaacacag aaaaaggaat aatctatttc ctacatgaag aaggttacga
     1381 tacccaaacc tttatgttta ctgaaaaaaa agaatttcat aaacagatga aaaaactgat
     1441 tgaaaaagaa tttccaagaa gtcataaact ctatgtgaat cagcaatttt aacttctaac
     1501 tgttcttta
//
LOCUS       DAYYHB010000217         1508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000217 DAYYHB010000000
VERSION     DAYYHB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.226"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1460
                     /locus_tag="ACHA6B_002890"
     CDS             <1..1460
                     /locus_tag="ACHA6B_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8950373.1"
                     /translation="QQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVAN
                     GGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLAL
                     PFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLA
                     PLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGF
                     IRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEE
                     KPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFI
                     FILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLL
                     GSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPG
                     TESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          410 a          289 c          318 g          491 t
ORIGIN      
        1 aacagcaaga caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg
       61 agatgtggga acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg
      121 atacagttgc caacggggga cttggattac cacgagcaac agcattagct atcatgtcga
      181 tctacggatc tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt
      241 taggagcaaa acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct
      301 tggctttacc atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa
      361 ccgccttttt gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact
      421 tgcgaagaga tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag
      481 caccactaat cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag
      541 ctgctattgg gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag
      601 acggagtggg tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc
      661 gtaacttgat catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg
      721 gtcatctgac aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt
      781 tgtattactt tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag
      841 tatgggctta tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag
      901 gttcatctat cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa
      961 cgatccaagc aagctggtat caatcattga atccaatttt catctttatt ctgacaccag
     1021 tattcgtaac gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct
     1081 ctcttggact tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt
     1141 atggtacaag tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta
     1201 ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag
     1261 catttgaatc acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca
     1321 atgcacaaat tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg
     1381 gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac
     1441 taatgggctc tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt
     1501 tcctgttt
//
LOCUS       DAYYHB010000218         1502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000218 DAYYHB010000000
VERSION     DAYYHB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.227"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..266
                     /locus_tag="ACHA6B_002891"
     CDS             102..266
                     /locus_tag="ACHA6B_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950374.1"
                     /translation="MGKGRNYVTNIINYYPNRLPKGSFKKVGDKTYIITEEGIEALRS
                     QTENKNKKKE"
     gene            263..>1502
                     /locus_tag="ACHA6B_002892"
     CDS             263..>1502
                     /locus_tag="ACHA6B_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8950375.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVS"
BASE COUNT          548 a          249 c          314 g          391 t
ORIGIN      
        1 tacaataaat tttgcgaaag gggtaggtct aaatcgcaaa caaaaagaaa ttgaacctag
       61 acaaatacaa cttgtacacc gcaagggaag caagtttgat tttgggtaaa ggtagaaatt
      121 atgttacaaa cataatcaac tattatccaa atcgtttgcc taaaggttcg ttcaagaaag
      181 tgggtgacaa aacctatatt ataacggaag aaggaataga agcattgcga tcacaaacag
      241 aaaataaaaa caaaaagaag gaatgaatat gaagaaaaat tcgcttggta tggttttatt
      301 tgcatcagct gtacttgcgg ggttatcagt accagtaacc tcacatgctg atgaatggac
      361 tgcaaacaca ccggatcaaa tccaaattac aacaggacaa tcaagttatg cgttcaaaaa
      421 aggagatact atttggtcaa ctgtagagaa gcttaaaagc ttaggttaca agactactgt
      481 tgctgagttt gccgaattga acaatgttga tttggcaaaa ggagaacaac ggactatccc
      541 aattggacga actttcaaca tcagtaaaga tacattaaca gttaaggaag cagatggcga
      601 agtagttagt caaactgttc aaaaagataa acctgatcct gttgaaaata cggagcaaga
      661 gaacaaaaaa tccgagactg caaaagaatc ggaaccaaca gcaaacactt ctgataagac
      721 agaaacaagt gcttcaagtg ttgatggagc aagttcatct aatacaaata attctacaag
      781 tgagaaagaa gcatcacagg gaacaagtga aagtagcact acggaaactg atactggaac
      841 atcaacaaat aactcacaat caacatcaaa tgatgcaaat gaatcaacca gtacatcttc
      901 tagtgatcaa tcacaaacag gttcgagtga tgaaactgac agttctactt caccaagtaa
      961 acccgaacaa ccaactaatg ttgtgctagg cgttccattt attagtcaag gtaacacaat
     1021 gctttgcgaa ggaacatctt tgctagaagc gttacattat aaaggtgttg caacaaatca
     1081 agatttaatg acttttgtta aatcaatgcc actatctcct gataataatc caaataatgg
     1141 tttttctggt gaatggagac acaatgtgga tggaacttat caaggtatga atccggctcc
     1201 tgtagtttca tggggtgttg ctaatggcgg aaatgttgta gatatttctg gttcatcggt
     1261 acaaggatta aaagatgaga ttgtaaatgg aaatcctgta gtagcgtggg taacttatgc
     1321 ctttgaacct gctcaacata aatcaatgtt ttggggcgat gctatttgga atcgacatgt
     1381 agtagccgtt gatggattta aagacggttt ttaccatgtt gtcgatcctg tgtttggggc
     1441 aagttggatt aatgcaggat catttgaaac agcatataac gtatctcaat tagcggtttc
     1501 gg
//
LOCUS       DAYYHB010000219         1423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000219 DAYYHB010000000
VERSION     DAYYHB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.228"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..1390
                     /locus_tag="ACHA6B_002893"
     CDS             98..1390
                     /locus_tag="ACHA6B_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFM8950376.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DAYYHB010000220         1404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000220 DAYYHB010000000
VERSION     DAYYHB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.229"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..>1404
                     /locus_tag="ACHA6B_002894"
     CDS             12..>1404
                     /locus_tag="ACHA6B_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8950377.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLA"
BASE COUNT          479 a          247 c          309 g          369 t
ORIGIN      
        1 agggaagata aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgatcc
       61 gtgtattggg ggaagggcat aatcagtgga cgtatcgtcc cgatctaaaa tcagaagaag
      121 atctttgggt caatttacgc caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ttcctttgac cgataaagaa tttgaaacga tcaaaacaga attgttacta cgaacgaaaa
      241 ccccctttga tgctgcgaaa tggttaaaag gtgaaaatgg aatggctcgg atcacgattg
      301 aacgggaaga tccgcaactg ggttctgtct cgctcattct ttattctaat caagacattg
      361 gtggaggaat ctctacctat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcacggga tcgacgtttt gacgtcacct tactgatcaa tggattaccg attgtccaaa
      481 tcgagctgaa acaagtaacc gcaaaagata gtttttatca ggcgtttaac caaatcaaaa
      541 aatatgcaga agaaggtatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaaccact cggtattttg caaatgcctt accaaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccggatgctc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa atcctcatgg tgttacatcc ttatcaagta catgcgattc
      841 aagccttgtt tattgctgca aataaacatc aatcaggata tgtgtggcat gcgactggtt
      901 caggaaaaac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcggacgat catgttagtg gaccgcaaag atttagataa tcaaaccacc acagagttta
     1021 cgaaatttgc ttcggaattt aatactggga tttcttccgg gaacgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 caaatgttgt gattattaca acccgtcaaa agctagatgc ggcgttgaaa tatgcaaaaa
     1201 aacaagaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagtaga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcac
//
LOCUS       DAYYHB010000221         1378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000221 DAYYHB010000000
VERSION     DAYYHB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.231"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..118)
                     /locus_tag="ACHA6B_002895"
     tRNA            complement(47..118)
                     /locus_tag="ACHA6B_002895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Gln,seq:ttg)
     gene            complement(128..200)
                     /locus_tag="ACHA6B_002896"
     tRNA            complement(128..200)
                     /locus_tag="ACHA6B_002896"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:His,seq:gtg)
     gene            complement(262..346)
                     /locus_tag="ACHA6B_002897"
     tRNA            complement(262..346)
                     /locus_tag="ACHA6B_002897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Leu,seq:caa)
     gene            complement(367..437)
                     /locus_tag="ACHA6B_002898"
     tRNA            complement(367..437)
                     /locus_tag="ACHA6B_002898"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Cys,seq:gca)
     gene            complement(483..554)
                     /locus_tag="ACHA6B_002899"
     tRNA            complement(483..554)
                     /locus_tag="ACHA6B_002899"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Gln,seq:ttg)
     gene            complement(566..638)
                     /locus_tag="ACHA6B_002900"
     tRNA            complement(566..638)
                     /locus_tag="ACHA6B_002900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:His,seq:gtg)
     gene            complement(641..714)
                     /locus_tag="ACHA6B_002901"
     tRNA            complement(641..714)
                     /locus_tag="ACHA6B_002901"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Trp,seq:cca)
     gene            complement(721..801)
                     /locus_tag="ACHA6B_002902"
     tRNA            complement(721..801)
                     /locus_tag="ACHA6B_002902"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Tyr,seq:gta)
     gene            complement(803..878)
                     /locus_tag="ACHA6B_002903"
     tRNA            complement(803..878)
                     /locus_tag="ACHA6B_002903"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Phe,seq:gaa)
     gene            complement(914..986)
                     /locus_tag="ACHA6B_002904"
     tRNA            complement(914..986)
                     /locus_tag="ACHA6B_002904"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(951..953),aa:Asp,seq:gtc)
     gene            complement(996..1068)
                     /locus_tag="ACHA6B_002905"
     tRNA            complement(996..1068)
                     /locus_tag="ACHA6B_002905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1033..1035),aa:Val,seq:tac)
     gene            complement(1084..1155)
                     /locus_tag="ACHA6B_002906"
     tRNA            complement(1084..1155)
                     /locus_tag="ACHA6B_002906"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1120..1122),aa:Glu,seq:ttc)
     gene            complement(1170..1258)
                     /locus_tag="ACHA6B_002907"
     tRNA            complement(1170..1258)
                     /locus_tag="ACHA6B_002907"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1222..1224),aa:Ser,seq:gga)
     gene            complement(1298..1371)
                     /locus_tag="ACHA6B_002908"
     tRNA            complement(1298..1371)
                     /locus_tag="ACHA6B_002908"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1335..1337),aa:Asn,seq:gtt)
BASE COUNT          394 a          366 c          312 g          306 t
ORIGIN      
        1 agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga
       61 ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag
      121 aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt
      181 aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa taaaaaacta
      241 agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc cctcaacggg
      301 cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt tgatttatta
      361 gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc tctgccttac
      421 cacttggcta taccgccatt atactattat caatctacaa aataaagaat aaattataga
      481 aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc
      541 ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga
      601 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt
      661 gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa
      721 tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt
      781 tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga
      841 gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat tattaattga
      901 ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
      961 catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct
     1021 gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta
     1081 aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
     1141 cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca
     1201 gtttgcacca tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag
     1261 agtgaaagaa gctcaatact ctttctcaaa atgagaactc cggcagtagg actcgaacct
     1321 acgacatcat gattaacagt catgcgctac taccaactga gctatgccgg aataacat
//
LOCUS       DAYYHB010000222         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000222 DAYYHB010000000
VERSION     DAYYHB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.232"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1340
                     /locus_tag="ACHA6B_002909"
     CDS             39..1340
                     /locus_tag="ACHA6B_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8950378.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DAYYHB010000223         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000223 DAYYHB010000000
VERSION     DAYYHB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.233"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHA6B_002910"
     CDS             82..>1364
                     /locus_tag="ACHA6B_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8950379.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYHB010000224         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000224 DAYYHB010000000
VERSION     DAYYHB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.234"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA6B_002911"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA6B_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8950380.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYHB010000225         1359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000225 DAYYHB010000000
VERSION     DAYYHB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.235"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..484
                     /locus_tag="ACHA6B_002912"
     CDS             <1..484
                     /locus_tag="ACHA6B_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8950381.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1215)
                     /locus_tag="ACHA6B_002913"
                     /pseudo
     CDS             complement(691..1215)
                     /locus_tag="ACHA6B_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          445 a          254 c          260 g          400 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaa
//
LOCUS       DAYYHB010000226         1332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000226 DAYYHB010000000
VERSION     DAYYHB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.237"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..690
                     /locus_tag="ACHA6B_002914"
     CDS             151..690
                     /locus_tag="ACHA6B_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950382.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          496 a          176 c          217 g          443 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg
      781 aatttattcc gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat
      841 aggaaaacgt agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa
      901 aattatataa actaatttag tgttttgttt gtaattccta attttgctcc acttttagat
      961 aatttttagg tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt
     1021 caactctttc gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta
     1081 cgatactagt atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat
     1141 agcattccta aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta
     1201 cttgaacatg aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt
     1261 gcgcaacatt tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa
     1321 tggcttgata at
//
LOCUS       DAYYHB010000227         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000227 DAYYHB010000000
VERSION     DAYYHB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.238"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHA6B_002915"
     CDS             278..820
                     /locus_tag="ACHA6B_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950383.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHA6B_002916"
     CDS             976..>1324
                     /locus_tag="ACHA6B_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8950384.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYHB010000228         1318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000228 DAYYHB010000000
VERSION     DAYYHB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.239"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..520
                     /locus_tag="ACHA6B_002917"
     CDS             281..520
                     /locus_tag="ACHA6B_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950385.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            577..705
                     /locus_tag="ACHA6B_002918"
     CDS             577..705
                     /locus_tag="ACHA6B_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950386.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(882..1142)
                     /locus_tag="ACHA6B_002919"
     CDS             complement(882..1142)
                     /locus_tag="ACHA6B_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950387.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          430 a          240 c          177 g          471 t
ORIGIN      
        1 atcctcctag caactattta aatacccaaa aaaattacct ctagataata tcatacctac
       61 gttttcactt ataaattttt tcataaaaaa agcgtttaga ggcataatcg ttaatacaga
      121 tggtaaccaa tgtatctggt ttgaactggg atatcaccct ctccatatat gtattactaa
      181 gactaataga tactataaaa gacttgatag ttttgcattg attttgccta atactgagca
      241 taggtgtatg ctaaacgata ctataaggag tgtgtacata atgaaaagaa ttctgtttat
      301 cagtttatca aatttattgt tacttactgc ttgtgggact tctgcatcaa agaacatacc
      361 ttcagatacg caaatttcga atagtatcga ttcaacaatt tctacaaatg agaaaaattc
      421 tccggtagaa acttccgata taaaagtttc tacctctgca aaaaacaggg taaagaaact
      481 gtcagcacgt ctcctttatt tcaaacaaac tctgaattag cttcttcatc ttcttcagca
      541 aataatgatg atagtttcgc gtttactcct aactaaatgc aacctttaca aatgctttac
      601 gataacaagc ctgagctaaa tgatcctgat atcagttttg cattttttga aatgattgga
      661 tcggatgatc tttttaaaac tactagtttg tctatccgaa aataggacag ttctggcagt
      721 ataggttttt atcgagtttc tccaccaggc ctcgttacag agacagattc tctaggcgaa
      781 ccttttgaat aaatcaaaaa tcatgaacaa tcactgccaa ctaaataaag tgccttcaaa
      841 agcttggtct tctcgctttt gaaggcactt tatttaaatc tttatgaaac taaagtatgt
      901 tcatatttgt tgctatagtt atatagatat tctgttgttt ccaactcata aacgtgagta
      961 tattttgcca ctacttttcc gcatttttct aatatttcat tgcgatcgac gtcatgatat
     1021 tcgtctacgc agatcacaac acaattttct aatttactga ttacttctcc aaccactttt
     1081 tgtttcaagc caataggttg gcaaatatat ttttttccta aaatgatagt ttcgttattc
     1141 atatttcatc catccttaat gtttttccat tatttaaaat gataatacca ttaaaatatg
     1201 attatgaaaa tcaataacac ttgtgatttt ataaaaaaat aaaaaaaccg aaaaacagaa
     1261 atgcccactg tctttccggc tctttttatt cttgatttct cttctttttt gtttcaat
//
LOCUS       DAYYHB010000229         1308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000229 DAYYHB010000000
VERSION     DAYYHB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.240"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1198
                     /locus_tag="ACHA6B_002920"
     CDS             158..1198
                     /locus_tag="ACHA6B_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8950388.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          529 a          182 c          210 g          387 t
ORIGIN      
        1 ttaaaggtag tacataattc attttatttc tgattttagg tagtatttaa ttaaaaaaca
       61 gttttaatgc gtttgcattt tttatttatc tttgctattt tcgaaaagag taggtaattt
      121 tacataaata aaacaacgtg aaagtagagg atctacaatg actgatttta aattttttaa
      181 ggcggataga gtttacaacg aattatttta tcaatttcca aaagtcttta ttgtttctga
      241 cgaatacaaa aaaatgaaag attcaactaa gattgcctat atgcttttaa aagcaagatt
      301 agagatcgca atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt ttacttatac
      361 gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa
      421 agagttggaa tcctttggtt tattattaca aaagcagatg ggatttaaca aacagttagg
      481 gaaaaataat cctaatagac tatatctagc agaattaaaa gtctcagaaa atgatatcta
      541 cttactcgaa aaatttgata gagagaatag ggaaaacgtt gataaatcag agggtatgaa
      601 aatcataccc accctcgacg aaaaatcaga cgctgaatcc cttggggctc aagagggtat
      661 gaaaatcata ccgtgccaaa acgttgataa atcagagggt atgaaaatca taccagaact
      721 taataataat atattagaca ctaatagaca caatatagac actgaaaaag accgcctaca
      781 agatcaattg ttgttagaca attttgagac aattatgaca aacgacagca ttgctacgtt
      841 tgtccctgaa cgagtattaa atttgataaa aacattttct tcaagttaca gtgaagctca
      901 aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct gaaatagaaa gtgatatttc
      961 gatagttttt gaagaactcg atagttatta tgtcaatgca gaacaagaat tatacacgac
     1021 actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat
     1081 ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag tatcaagtga
     1141 aaaacgtttg aattatgcaa gctccccaag cactattacg aaagactggt tagagtgaaa
     1201 ctttactgac aaagtgattg ttgcttcatg gagtaacatt aaaattaaaa attttttatt
     1261 attctaatta tattacgaat attacaaaat aatagctatt ttaataga
//
LOCUS       DAYYHB010000230         1275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000230 DAYYHB010000000
VERSION     DAYYHB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.241"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHA6B_002921"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHA6B_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHA6B_002922"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHA6B_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          451 a          214 c          174 g          436 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaat
//
LOCUS       DAYYHB010000231         1201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000231 DAYYHB010000000
VERSION     DAYYHB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.242"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..348)
                     /locus_tag="ACHA6B_002923"
     CDS             complement(61..348)
                     /locus_tag="ACHA6B_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950389.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(361..1179)
                     /locus_tag="ACHA6B_002924"
     CDS             complement(361..1179)
                     /locus_tag="ACHA6B_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8950390.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT          339 a          251 c          164 g          447 t
ORIGIN      
        1 tttagttaag ctatacctaa ccgcttattc caataaaaaa agtgatcagc tttaaactga
       61 tcactcacca aaaacttcat catacaagtt atctatcatt tcaccaagta aagctgaatc
      121 tgatcgaatt cctaactttt cgctgatttt agatagtttc tttctttgcg atggttgaag
      181 cgtgaattga taatttttct tcttttcaga actatcactt tttagtttag ctaccaaaga
      241 acttgttgtt tctttaggtg tttgtttagg ttcttcaact gcttcaacca cttcaggaac
      301 ttcttctact tcagcttcag cgaatttttt aaatctattt tctttcactt caatcactcc
      361 ttattcttgt gccgtatcaa ttattttgtt gtatagcgta aacgtgcttt caaagaaatc
      421 cttattctta cgatagatat tcttatcctt agcgaactca gcaatattct taccttctaa
      481 gctagttgca ttaagcaatt ccttctcttg tacatatcca agataattag ataacgttgg
      541 taccagttct aggaactcac gacttgaatt agtattatgt ttgatcctgc taccgatcgt
      601 agcgtattta gtttccatca gtgtttcacc agtcttaata tcaatcaact gttttgctag
      661 tgcatcaata tctttaccaa gcttcagcaa agcttcaaaa ccatctttag atggatcaga
      721 aatgcctacg acaagatcag atacagccca agcattttga gctagtagac ctgcatcatt
      781 atgagtatca atcagaatta gatcatactt ctctttaagc tgttcatagt taacaaccat
      841 ccatctaaac agtagggttc tgttgttagg catatcacgc atttgcatct caacttgttt
      901 taaatcttca cctgctacta aaatatccat gttctcattt acagctaaag gttcagctga
      961 tccatgaaga tataaatgat tgattgtatt ctctcttttc gctttactga aagcatcagg
     1021 aaatcgtttt gataggtttt gcgacttatc ttcatcaata tacagcacct tcatacctaa
     1081 attttcgaca gcatatccac ctagattgta gattgttgtt gttttacctg ttccaccttt
     1141 ttcaatgttt ccagtaatga ttgacaactt cttttccatg attaaacctc ccttattaac
     1201 c
//
LOCUS       DAYYHB010000232         1199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000232 DAYYHB010000000
VERSION     DAYYHB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.243"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1199
                     /locus_tag="ACHA6B_002925"
     CDS             <1..>1199
                     /locus_tag="ACHA6B_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950391.1"
                     /translation="FFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLFIFLPV
                     AGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVPDGKPT
                     SEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTFNINTY
                     AGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTDDNRKP
                     DGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGIQGSAK
                     NMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIVKAFKR
                     EFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAVGFIPN
                     TLVGDGVA"
BASE COUNT          367 a          206 c          263 g          363 t
ORIGIN      
        1 tttttcacag caatactagg ttatcaagga atgaaattat tgtcgaaaga tggtgttaac
       61 tcgaagggat tatcaccagt acttgaaaaa tggattgttc gtatttttgt attgtttata
      121 ttcttaccag tggcaggatc gctattggga gtttatatca acggcatcca gtcagcaaaa
      181 aaagatggag atataccaac aaatgttgtt tctgactaca tcattaacac aagactttgg
      241 gcttctactc aaaatcttgc tccatcatct gatactggtg gtgatgtacc tgacggaaag
      301 cctacatccg aaatcaagtt cgttgataca gattatgaac catctaaaaa atctgcaatg
      361 ataagagcga tcaatactag cagttatgcc agacaaaata atgatctaga ttctaattca
      421 gtcggtatgg ctctattaaa agaatggtca tcgaatgata ctttcaatat taatacttat
      481 gccggtgatg ttaatgtcgc cgaaaatgat gcttatatgg gaagagcgcc tgctgtttgg
      541 acaggctata atttagggga tggaaaagaa ggaagtgcaa ttaagaaacc aaccaattta
      601 aatggatata tttggtcagc aaatcaaaat gtgacagacg acaatcgcaa gcctgatgga
      661 aaaggaacag cgcataactt tgatgctact caaccttggg gcgttgctaa caatgcaact
      721 tttagcacac agtcggtagc tttattgctc caatcggcac ttgatacatc gggagctaag
      781 ttttatgcgt ataatatcgc tccttcagga attcaaggca gtgctaagaa tatgtcaaca
      841 gtaaaaacag aatggcgtac agcatcctta gttggtcatg gagctatggg taagtttggc
      901 tcgtggctag cgatgaacgc tgaatcaatc gttttagcag ttctttactt agcatgtgct
      961 tgggcgatgt tcaatgtgaa tatcgtgaaa gcatttaaac gtgaatttat tcggatttgg
     1021 gaagtagttc taacaggatc acttgcatca ggagcaggtg tgatggcaat ttcattaggt
     1081 gttgttacaa catctctgat cggactacaa ttacctatct ttctgattac ttttattcgt
     1141 gcatttggtg atactgctgt gggattcatt cctaatacat tagttggcga tggcgtagc
//
LOCUS       DAYYHB010000233         1184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000233 DAYYHB010000000
VERSION     DAYYHB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.244"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            319..>1184
                     /locus_tag="ACHA6B_002926"
     CDS             319..>1184
                     /locus_tag="ACHA6B_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFM8950392.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          443 a          145 c          230 g          366 t
ORIGIN      
        1 ctttcgtcgg aaatacaatt tgagtgtacc ataaatgcct ttttattttt ctaacttaat
       61 tttacaattc gcgaaataaa aaaagcacct gaaaaggtgt ctttttttga tggttttgaa
      121 cttgttcttt cttatcttga tacatataga aataacgtca tttttatttt agttgctgaa
      181 aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct taaaattggt
      241 tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac taaatagatg
      301 ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga aaaatcaagg
      361 gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct
      421 aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga aagagtaaaa
      481 gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat
      541 cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag agcaatgaaa
      601 catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc aacagttcgt
      661 tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt aaataagagt
      721 ttgtcagata tggctcaagg atttcgccga atgatgcaat ataaaaaaat taataaaaat
      781 cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa ttcttataat
      841 cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac agaaaactac
      901 gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga ctatgatcca
      961 aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat acaatcggca
     1021 attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga tgatgaagaa
     1081 aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag gttaatctcc
     1141 tatggtggtt tgttaaaaga aatacataaa aaattaaacc ttga
//
LOCUS       DAYYHB010000234         1164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000234 DAYYHB010000000
VERSION     DAYYHB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.245"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..838
                     /locus_tag="ACHA6B_002927"
     CDS             <1..838
                     /locus_tag="ACHA6B_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8950393.1"
                     /translation="KGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEG
                     ISVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATG
                     LATFNIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHK
                     VDDQGKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKW
                     SKEGITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHK
                     AK"
     gene            887..>1164
                     /locus_tag="ACHA6B_002928"
     CDS             887..>1164
                     /locus_tag="ACHA6B_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950394.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYV"
BASE COUNT          461 a          185 c          245 g          273 t
ORIGIN      
        1 aaaaggtgca acacaagtag aaaaaacaga tgaaaatgga cttgcaagct tcaatattgt
       61 agcaaacaag acgtatagtc tagaagaaac cggagtacca gtaggttaca caggtgcatt
      121 taagcaagag ggtatcagtg ttaaagacga tgggcaaact tttgaatata cagctacaaa
      181 cactttgaaa aaaggtgtag taaaagttca taaagtcgat caagatggaa aacctttagc
      241 agatgcagaa ttcaccttaa cggatgaaaa aggtgtaaaa gaagttaaaa agacggatgc
      301 aactggtctg gctacgttca acattattgc gaacaaaact tacaacttag aagaaacaaa
      361 agtaccaaca ggttacacag gcgcattcaa acaagaagga attaccgtca aagatgatgg
      421 acaagtcttt gaatatacag ctaaaaacac tttgaaaaaa ggtgtgatca aagttcataa
      481 agtcgatgat caagggaaag cattagcaaa cgcagaattc actttgacgg acgacaaagg
      541 tgtaaaagtt gtacaaaaaa cggacagaaa tggcttggca acatttaata ttgttgccaa
      601 caaaacgtac aaaatcgaag aaacacaaaa ccctaatggt tatacaggga aatggtctaa
      661 agaaggcatc accgttgctg acgatggaca aacctttgaa tatacagtaa ttaatcacct
      721 tgtacctgaa acagtaagaa caggcggaat cggtacatca gccttccttc ttgtatttct
      781 aattgcagga ttgagtatgg tgctactgta ttatcgagaa cagcacaaag caaaataaaa
      841 aagtgttaat ggaaaagaag taaatatgaa aaaagaaggc tgacgtatga aatcaaaaaa
      901 aattttttca aatttaatgc tatttggtac tttgctagga gtgggattgg gcgcaagctc
      961 aatctcttct agccttggac taggggggca agtagcatat gcacaacaaa actataatta
     1021 tcaaggaact ggaacgattc atatccataa agtaacaaat actaatgggg atgggaaccc
     1081 tgtctcagat attgataaca aagggaaaga aatagcttta ccaaatggtg taaaacctct
     1141 tgaaggcgtt gagttctctt atgt
//
LOCUS       DAYYHB010000235         1153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000235 DAYYHB010000000
VERSION     DAYYHB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.246"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..923)
                     /locus_tag="ACHA6B_002929"
     CDS             complement(99..923)
                     /locus_tag="ACHA6B_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950395.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(947..1102)
                     /locus_tag="ACHA6B_002930"
     CDS             complement(947..1102)
                     /locus_tag="ACHA6B_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950396.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT          370 a          192 c          131 g          460 t
ORIGIN      
        1 taagtcttac tcagaacaga taaagcataa aattccaaca aaccattttt tacttaacta
       61 aataaagagt ctgttaatat aatcttcact tatttacgtt aatcccctac tgtatttgtt
      121 ggttccttat ctatattttc tacaaaatcc ttattatctt gtgatttagc ttcgtataat
      181 tctttgtatg tcgacacatt ggttatacta attcctatag ctagttcttg agactctact
      241 tttctctcta gctcctttga caaagtgatt tgagatttat ttggttccgt tggagtcaat
      301 tctatagatt cttcataaaa atctctatac ataaagctat cattaatttt ataaaaaaaa
      361 ttaaatgttt ttagcactgc catataacat tcaaaacatt cttctatagt tggtttactt
      421 ccttgaggag aatgaaccaa tttattacgt ttttctatga aatttttaat ttcctttctt
      481 atttctttcg gaaactcaat attaaagtac cctttaaaca tgctccctaa taatttgtct
      541 acagggggag attgtaaatt ctcaaatatt agagttaaat caggtttctc ttgttgataa
      601 aattccttta cccctatttc acaggctgta attatcgaaa taaacctatc tctagtattt
      661 gatatatttt tacttctttt aaggagtata agagaaaagg ggatactttc gtgtccatgc
      721 atgtaattaa ctaattgttg agcttcagaa gttatatctc taattatata gttaacggaa
      781 ttaggaagtg tctttaactc taatgaaatt tctatccaat tactatcttc taatctgtat
      841 atgctcgaag tataatttga ttcgtcagca agcatcgcta catcccaaac actgatcata
      901 aaatctttta aacgttcaat catttcagca atattgctaa aatcagctaa tttttcttta
      961 gaataaaaaa aacatttata tcagaatcaa taaattctat tttttttatt actatatcgt
     1021 catcattatc ttgaccattt atgtttattg ttccctgagt ggggagtact tctttcaaat
     1081 tcaagttaaa tttatattcc attacttctc ctccaatata taaaaattac ttaactgtat
     1141 ccaaatttaa tat
//
LOCUS       DAYYHB010000236         1138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000236 DAYYHB010000000
VERSION     DAYYHB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.247"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..530
                     /locus_tag="ACHA6B_002931"
     CDS             189..530
                     /locus_tag="ACHA6B_002931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950397.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            898..1089
                     /locus_tag="ACHA6B_002932"
                     /pseudo
     CDS             898..1089
                     /locus_tag="ACHA6B_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          416 a          126 c          188 g          408 t
ORIGIN      
        1 gtgatgatgc tcttacattc ggtaaaacta ctttttctag ttttaatttt cagtttctat
       61 taaaaaataa tttttatggt ttattgaaat aaggttgcga aggaagatgt agagttattt
      121 gcactgttgt gttggtatat ctcttaatgc tgtgtctttt tggggagatt aattagtgga
      181 gtgattatat gagtatagat gattgtttag aagaacaaat aagattattt ttaaagtttt
      241 taaaagttaa agttcaaaat aactacttca catgttatag ttatgcacac cctgttttaa
      301 atagtcttcc ttttaattct ttagtgcaag gattgattac aaatattgtt ttattagtct
      361 gtacaccaaa tgaaatattt gtaaaaaaga taggcagtgg tatttcttca gtaagattaa
      421 aagagaaaga atatactcaa aagattattc atattcctaa gcctgaaatt aagcgttttg
      481 agataaaaga ctattctgct ttagggctgt tctcaattgg ttactggtga taaaaaataa
      541 gaatactaaa actatacgta gaatataagt aatcggcatc aatcctaatt atgtgattga
      601 atgccgattt ttgttacgcg ctatttttaa ttatctgtaa gaagaataat tttattttac
      661 tcatattcgt tttcactttg aagaagtaaa ttgcaatttc ttataagtta agcagctatg
      721 caaaaggcta tttattctat tgtgaaatat aagatgtagt tgaaacagga tatatagaat
      781 caaaatacga atcttatttt gatttttcta gttttttgaa aaaagcaccc tttgatcgtt
      841 gtattagtgg aaaagagaaa aaggagcata cttatcatgt cacaaaacta caactaaata
      901 tctaaacaac accaatttga tgcctattga aaaaaagtat tgcgaaatga agccaagtct
      961 attagaaaat gaaataatag aataaaagaa aatgggaaac cgctgaatga tgtaaatgaa
     1021 ggtaagtatc cacgaactaa ttttgatgaa caagatatct attttttatt tgtattggag
     1081 atactagttt aatatcaaaa gttatcagca gtcataatta gttatccaat gtaaaaag
//
LOCUS       DAYYHB010000237         1115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000237 DAYYHB010000000
VERSION     DAYYHB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.248"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..77
                     /locus_tag="ACHA6B_002933"
     CDS             <1..77
                     /locus_tag="ACHA6B_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8950398.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            82..1029
                     /locus_tag="ACHA6B_002934"
     CDS             82..1029
                     /locus_tag="ACHA6B_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8950399.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          352 t
ORIGIN      
        1 tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt
       61 atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt
      121 ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta
      181 tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat
      241 gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt
      301 catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt
      361 gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta
      421 aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa
      481 aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa
      541 caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc
      601 aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc
      661 gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat
      721 ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt
      781 gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca
      841 cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac
      901 aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca
      961 attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa
     1021 atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg
     1081 actgatgagt tttgtaaaat caaatctggg ataga
//
LOCUS       DAYYHB010000238         1080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000238 DAYYHB010000000
VERSION     DAYYHB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.249"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1080
                     /locus_tag="ACHA6B_002935"
     CDS             <1..>1080
                     /locus_tag="ACHA6B_002935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8950400.1"
                     /translation="ETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDI
                     PGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLF
                     DVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSM
                     DMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRS
                     SVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSE
                     SIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIE
                     EQITLDKLKMLDYLFHPSFSQPYNF"
BASE COUNT          415 a          131 c          184 g          350 t
ORIGIN      
        1 agagacagaa gttgacaaaa ttgatattca aaataaaaag attagttgtg taaaaaaaga
       61 tggtacaaga ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat
      121 tatgctagat ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa
      181 tgcaaaagag atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg
      241 agcaggatat attggagttg aaattgctga agccataaga aaaagaggga aagaagtata
      301 tcttttcgat gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa
      361 agttgaagaa atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa
      421 atatttagga agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat
      481 ggatatggtc gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact
      541 cttgagagat gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga
      601 tatatatgct attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt
      661 tgatttttct atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa
      721 tcaagatttt acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa
      781 tttttacgca gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta
      841 tatagaaata gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt
      901 aagaattagc tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga
      961 agatttatct ggactattat caatgttttc tcttgctata gaagaacaaa ttacattaga
     1021 caaattgaaa atgttagatt atttatttca tcctagtttt agccaaccct ataattttat
//
LOCUS       DAYYHB010000239         1073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000239 DAYYHB010000000
VERSION     DAYYHB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.250"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACHA6B_002936"
     rRNA            <1..>1073
                     /locus_tag="ACHA6B_002936"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAYYHB010000240         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000240 DAYYHB010000000
VERSION     DAYYHB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.251"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1001)
                     /locus_tag="ACHA6B_002937"
     rRNA            complement(<1..>1001)
                     /locus_tag="ACHA6B_002937"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAYYHB010000241         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000241 DAYYHB010000000
VERSION     DAYYHB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.252"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1001
                     /locus_tag="ACHA6B_002938"
                     /pseudo
     CDS             369..1001
                     /locus_tag="ACHA6B_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          343 a          182 c          182 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccactact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DAYYHB010000242          989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000242 DAYYHB010000000
VERSION     DAYYHB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.253"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            400..>989
                     /gene="mobV"
                     /locus_tag="ACHA6B_002939"
     CDS             400..>989
                     /gene="mobV"
                     /locus_tag="ACHA6B_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFM8950401.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTL"
BASE COUNT          356 a          151 c          213 g          269 t
ORIGIN      
        1 aaaaccgaaa gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca
       61 aaacggacaa aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct
      121 caaaaaatac tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt
      181 tgctgaggtg gcagagggca ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt
      241 cttaaaggtt ttatggtttt ggtcggcact gccgacagcc tcgcagagca cacactttat
      301 gaatataaag tatagtgtgt tatactttac ttggaagtgg ttgccggaaa gagcgaaaat
      361 gcctcacatt tgtgccacct aaaaaggagc gatttacata tgagttatgc agtttgtaga
      421 atgcaaaaag tgaaatcagc tggactaaaa ggcatgcaat ttcataatca aagagagcga
      481 aaaagtagaa cgaatgatga tattgaccat gagcgaacac gtgaaaatta tgatttgaaa
      541 aatgataaaa atattgatta caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca
      601 ggtacaagaa aaacgaggaa agatgctgtt cttgtaaatg agttgctagt aacatctgac
      661 cgagattttt ttgagcaact ggatccagga gaacaaaaac gattttttga ggaaagttat
      721 aaattatttt ccgaacgata tggcaagcaa aatattgctt atgcaacagt tcataatgat
      781 gagcaaaccc ctcacatgca tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga
      841 aaaaatgtgt ttaatcgtca agaactgtta tggctacaag ataaattccc cgagcatatg
      901 aaaaaacagg gttttgagtt gaagcgtggt gaacgtggct ctgaccgtaa acatattgag
      961 acagctaaat ttaaaaaaca aactttgga
//
LOCUS       DAYYHB010000243          983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000243 DAYYHB010000000
VERSION     DAYYHB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.254"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>983
                     /locus_tag="ACHA6B_002940"
     CDS             <1..>983
                     /locus_tag="ACHA6B_002940"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8950402.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQK"
BASE COUNT          375 a          159 c          188 g          261 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gat
//
LOCUS       DAYYHB010000244          970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000244 DAYYHB010000000
VERSION     DAYYHB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.255"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..922
                     /locus_tag="ACHA6B_002941"
     CDS             <1..922
                     /locus_tag="ACHA6B_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8950403.1"
                     /translation="KEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKL
                     TISNIDSTRGKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQ
                     QKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANK
                     IQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRI
                     KELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEK
                     VVLENYNGTVTFTKLAKAEQYRRSLELSKD"
BASE COUNT          420 a          105 c          163 g          282 t
ORIGIN      
        1 aaaagaaaag gaatatatta ttgacgatgc tgtttctgtc cctcttattt taaataaacg
       61 agagaaaaat aatttaaaga aaaaaatgct cattccattt gattcagaag tgaaaggatt
      121 actcaataaa ctaactattt ctaatataga ctcgactagg ggaaaaatca taaaaatatt
      181 tgataaaatt aataatttat tcaagaatga caatgctgaa ttaaggaagg gtcttttaga
      241 gtttaaactt caagaactag aacttcatta ctcttattta gtgaaaatta ccgaagaaaa
      301 agaacaacaa aaagcaataa aagaacagat gatagaagag gaaaaagttc gtagagaaat
      361 tgatcgtgaa aagaaacgta ttgataaaga acaacgtcaa ttcaattctg aaatttccaa
      421 attaatcgca tatatgcaaa aagctaatgc agatgtggaa aaagaattat atgcaaataa
      481 aattcaagaa ctggaagaaa aattaaaaga attagaagtt atacgtgaaa atgtattaca
      541 aagagaatta aatactagag ccggttatgt gtatgtaatt agtaatattg ggtcttttgg
      601 tgaagacata tataaaattg gtatgactag aagattagaa cctatggata gaataaaaga
      661 attgagtagt gcatcggttc catttgaatt tgacgttcat gcgatgattt tttcagagga
      721 cgctccgtct ttggaaacta aattgcataa tcattttaga aaacaagaag ttaataaaat
      781 taatcaaaga aaagaatttt tcaaagtttc actagacgaa atagagaaag ttgttttaga
      841 aaattataat ggaacagtaa catttacaaa attagctaaa gctgaacaat atagacgatc
      901 tttagaattg tcaaaagatt aaatatataa aaagccttcg ggctttttct tttaacaatt
      961 aataggaacg
//
LOCUS       DAYYHB010000245          957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000245 DAYYHB010000000
VERSION     DAYYHB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.256"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..884
                     /gene="galU"
                     /locus_tag="ACHA6B_002942"
     CDS             3..884
                     /gene="galU"
                     /locus_tag="ACHA6B_002942"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8950404.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          345 a          162 c          221 g          229 t
ORIGIN      
        1 aagtgaaggt caaaaaagca gtcattcctg ccgctgggct aggaacacgt tttttgccgg
       61 caacaaaagc catggcaaaa gaaatgcttc caatcgtgga taaaccaacg atccagttta
      121 ttgtggaaga agcattggat tcgggaatcg aagacatctt gatcgtaacg ggaaaagaaa
      181 aaagacccat tgaagatcac tttgatgcaa atatcgaatt ggaaaacaat ttgcgggaaa
      241 aggaaaaaac agaattactc gctttagttg aagaaacgac tcaagtcaat cttcacttca
      301 tccgtcaatc tcatccaaga ggactggggg atgccgtgct tcaagcaaaa gcatttatag
      361 gaaatgaacc gtttgtcgtc atgctggggg atgatttgat gaaagatgat atcccattga
      421 ctcaacaatt gatgatggat tatgaagaaa cacaagcttc cgctgttgcc gtcatgcgtg
      481 taccagaaaa tgaaacatct aaatacggaa ttattgatcc taaaacagat agcgaaaagg
      541 gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat gggaaaagca ccaagtaatt
      601 tagccattat cggacgttat ctactgactc ctaaaatatt tgagatatta gaaactcaag
      661 aaccaggagc aggaaacgag atacagctaa cggatgcact tcagactctt aaccagacag
      721 aggcggtcta tgcgagagag tttaaaggaa aaagatacga cgtaggcgat aagttaggat
      781 atatgaagac aaatattgaa tatggacttc aacattcgga aattgggact tcattaagtg
      841 catatattat ccagttgagt aaaaagctaa gaacagaaag ttaactggaa ataagtggcc
      901 tgctgagaat gaaaggctat ttgttattaa agcggcagct tgtgcgtcat ttgcgca
//
LOCUS       DAYYHB010000246          956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000246 DAYYHB010000000
VERSION     DAYYHB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.257"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..568)
                     /locus_tag="ACHA6B_002943"
     CDS             complement(38..568)
                     /locus_tag="ACHA6B_002943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950405.1"
                     /translation="MHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELL
                     QDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVI
                     EAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKI
                     DKQIDDFPEEIIDRIE"
     gene            complement(662..>956)
                     /locus_tag="ACHA6B_002944"
     CDS             complement(662..>956)
                     /locus_tag="ACHA6B_002944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950406.1"
                     /translation="KMLKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSL
                     FFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNE"
BASE COUNT          309 a          162 c          144 g          341 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcattcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttt
//
LOCUS       DAYYHB010000247          955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000247 DAYYHB010000000
VERSION     DAYYHB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.258"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..346)
                     /locus_tag="ACHA6B_002945"
     CDS             complement(152..346)
                     /locus_tag="ACHA6B_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950407.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
     gene            594..857
                     /locus_tag="ACHA6B_002946"
     CDS             594..857
                     /locus_tag="ACHA6B_002946"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950408.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTILKKALTIFAQK"
BASE COUNT          322 a          186 c          172 g          275 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgatttggta gaatctaact actaagaaaa ggacgtgtcc acgatggcaa
      601 aagccaagaa aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg
      661 gccaatcggt cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt
      721 ggaagaagct tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga
      781 tgaaaaagga aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca
      841 tattcgctca aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga
      901 tcatgacgta aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actat
//
LOCUS       DAYYHB010000248          942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000248 DAYYHB010000000
VERSION     DAYYHB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.259"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..829
                     /locus_tag="ACHA6B_002947"
     CDS             68..829
                     /locus_tag="ACHA6B_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8950409.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          251 a          155 c          189 g          347 t
ORIGIN      
        1 aaatgtgatt tttttcacta tattttatca catgaaaatt cattaagaaa gtaggaatcg
       61 aagtattatg cacacaccag aagaaattat ggaactcagt attcatcttg gaacaaaaaa
      121 aatacataaa cctatcttag ctaaactgat tttaggtttt atcggaggag ccatgatttc
      181 tctaggttat ttggcatacg tacgagtctc tgcttctatc ccagaatcta tggctagctt
      241 gactagttta gttggtgcct cggtttttcc tatcggcctg ctggtcatcc ttcttggcgg
      301 tggtgaattg atcaccggaa atatgatggc tgtggcgatt gcctttatgg ataaaaaagt
      361 cacattctca cagctggtta agaattggtc agtgattact tttgcaaata ttattggtgc
      421 aattttcgtt gcctatttct ttggacactt tgttggttta actcattcag gtgtctatct
      481 cgaccaagtg attcatattg ctgatcataa aatcgctgtt tcttggtggc aagcattagg
      541 ctccggaata ggttgtaatt ggtttgtcgg tttagcgtta tggttatcat atggagcaaa
      601 agatgcggcc ggcaaagtac taacgatttg gtttccagtc atgatttttg tggcaatcgg
      661 tttccagcat agtgtggcca actgttttgt gatacctgct gccatatttg aaggtcaagc
      721 gacttggtta gaatttctct cgaattttat ccccgtttat attggaaata tcatcggcgg
      781 agctattttt gtttcaggtt tttattacgt tagttataag catcattaag attgaaggta
      841 atgttcattc gagcattacc ttcaatctct ttatatacta tttatatttt aagaacaata
      901 gccttttatt atatatatta ttcaaattag ttatatcgag tg
//
LOCUS       DAYYHB010000249          941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000249 DAYYHB010000000
VERSION     DAYYHB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.260"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..237)
                     /locus_tag="ACHA6B_002948"
     CDS             complement(<1..237)
                     /locus_tag="ACHA6B_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM8950410.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISF"
     gene            complement(230..>941)
                     /locus_tag="ACHA6B_002949"
     CDS             complement(230..>941)
                     /locus_tag="ACHA6B_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8950411.1"
                     /translation="EKYLKYVLLSGADYPIKDNEYIYNYFKKNSSVEFIRGIDLDQIK
                     HKEFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSD
                     KLLNRIDKEILKVGNIDE"
BASE COUNT          333 a          148 c          101 g          359 t
ORIGIN      
        1 aaaactaatc attgctccaa tcagtagcca agaaaaacgc attttaaaaa aatttttctt
       61 tataaaaacc tcatacaaaa ctataattcc accccaagca aaagctattt tcatcgggat
      121 attagttaca ttaaaagtac taggcataaa ataaagcaga caaatgatta aatttattac
      181 tcgaaacgca tctcgactta ataaactctt ctctaaaaca ggtactattc tattcatcta
      241 tatttccaac tttcaaaatt tctttatcaa ttctatttaa tagtttatca gagtaatccg
      301 atttaacttt tcttacaaat aatttttcac tttcttttat ttgatctata tccctttcat
      361 caaaccattt gttcatggaa ggatcaataa tatgaatatt tgctaatttc gaagtttgta
      421 aagtagtttc ttcaatattt ttcaattcaa gtggtaaatc tggtcctcca ataacatttt
      481 tatttttgaa cgaggaatta aaaaaaagtg tatgaaaaaa tttttcatct ggggcaaaca
      541 tacctatttt aaaattcagg tagtcttgtt gattttgttc atacatttgt attaattctg
      601 ttaggcattc tttcgacaaa gcccaccatt gagaaccatg atacaaatca aatttatggt
      661 gtctaatttt tgggggaagt ttaatcatcc tcagacaacg attaattata gctctaaaaa
      721 ttttaaatgc agttgtgtta ttcctattaa ttctaggata atcatgtttt tgatatacat
      781 ctatatgttt ataataaaat tctttatgtt taatttgatc tagatctatt ccacgaataa
      841 actccactga actattcttt ttaaaataat tataaatata ctcattatct ttgattggat
      901 aatcagcacc agataataat acatatttta aatatttctc a
//
LOCUS       DAYYHB010000250          934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000250 DAYYHB010000000
VERSION     DAYYHB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.261"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..191
                     /locus_tag="ACHA6B_002950"
     CDS             <1..191
                     /locus_tag="ACHA6B_002950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950412.1"
                     /translation="FFIYLISAQKMCMVTVKGKVIKGMLATLTILGYHHIFIMNDLFV
                     YQTTHIVQRKSRLGTWLF"
     gene            complement(392..>934)
                     /locus_tag="ACHA6B_002951"
     CDS             complement(392..>934)
                     /locus_tag="ACHA6B_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8950413.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
BASE COUNT          269 a          155 c          156 g          354 t
ORIGIN      
        1 atttttttat ttacttgatt tctgctcaaa aaatgtgtat ggtaactgta aaaggtaaag
       61 taattaaagg tatgctagcc acgcttacta tattaggata ccatcatata tttataatga
      121 acgatttgtt cgtctaccaa acaacacata tcgttcagcg taagtcacgt ttaggaacgt
      181 ggctttttta gtgttattat cgataaataa tctattgact ctattgattt tatctccata
      241 tccctacggt gttgattgtc tttagatctt tcatttaaga accaaataac tatcataaaa
      301 atcagcactg ctgctaatat aataaacagc attcctattg tgatattact ggatttgagt
      361 caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt gctaattctt
      421 caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg ttataaaatc
      481 cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag ctagtcgtgt
      541 agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta tcataaggtg
      601 ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt tccacttctc
      661 ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga atgtgataat
      721 gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt tttgaaaatg
      781 tataactaat aattcttttc gtgtgtaaat ccatgattga agataagtaa caccaaccat
      841 ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc gtttcagtag
      901 aaaagtcctg atttaagaga ttctcttttg aaat
//
LOCUS       DAYYHB010000251          929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000251 DAYYHB010000000
VERSION     DAYYHB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.262"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>929
                     /locus_tag="ACHA6B_002952"
     CDS             <1..>929
                     /locus_tag="ACHA6B_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8950414.1"
                     /translation="MYNPNSGEHLYTRSVGERDNLKRYGWHYEGVAWQAPTSGTPVYR
                     LYNRYNGEHFYTVDAGERDKISHTGWTYEGVAFYSYNGANRVPVTRLYNKYVNWHHYT
                     LDQNEKNVISKQGWKIEGTGWYAMPNSVPNPTPAPSTEKVLNVPVVWQGNTMLCEGAS
                     LLEGLHYKGVATNQSLTSFVNYMPKANDNNPYHGYSGEWRHNVNGTYQGMMADPVVKW
                     AKNVGGNAVNITGSGTNGIKNEIRNGNPVVTWITYNYATPEFKQMPWGRAVWNGHVVL
                     VDGFRNGAYHVVDPVFGAKWINASTYERAFNVT"
BASE COUNT          306 a          163 c          218 g          242 t
ORIGIN      
        1 gtatgtataa tcccaatagc ggagagcatc tatacactag aagcgttgga gaaagagaca
       61 atctgaaacg ttatggatgg cattacgaag gtgtcgcatg gcaagctcca acaagtggta
      121 ctcctgttta tcgtttgtat aatcggtata acggtgaaca tttttacaca gttgatgccg
      181 gagaaagaga taagatttca cacacaggat ggacgtacga aggtgttgcg ttctattcct
      241 acaacggagc aaatagagtt cctgtaacac gtttatataa caaatacgtg aattggcatc
      301 actacacgtt agatcaaaat gaaaagaacg taattagtaa acaaggttgg aagattgaag
      361 gtacaggctg gtatgcaatg ccaaacagcg taccaaatcc tacacctgct ccaagcactg
      421 aaaaagtgtt gaacgttcct gttgtatggc aaggaaatac aatgttatgc gaaggagctt
      481 ctttattaga aggtttacac tacaaaggtg tagccacaaa tcaaagctta accagttttg
      541 taaactatat gccaaaagct aatgacaata atccttatca tggttactca ggagagtggc
      601 gtcataacgt taatggaacg tatcaaggca tgatggctga cccagtagtt aaatgggcaa
      661 aaaacgttgg cggtaatgcc gtcaatatca ctggttcagg aacgaatgga atcaagaatg
      721 aaattcgcaa tggtaatccg gttgtaactt ggattactta taactatgct acgccagagt
      781 tcaaacaaat gccttggggt cgtgcagtat ggaatggaca tgtagttctc gtggatggct
      841 tcagaaatgg tgcataccat gttgttgacc ctgtatttgg cgcaaaatgg atcaatgcaa
      901 gcacgtatga gagagcattt aatgtgact
//
LOCUS       DAYYHB010000252          907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000252 DAYYHB010000000
VERSION     DAYYHB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.264"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..>907
                     /gene="bsh"
                     /locus_tag="ACHA6B_002953"
     CDS             5..>907
                     /gene="bsh"
                     /locus_tag="ACHA6B_002953"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8950415.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNK"
BASE COUNT          311 a          121 c          164 g          311 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaa
//
LOCUS       DAYYHB010000253          896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000253 DAYYHB010000000
VERSION     DAYYHB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.265"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..886)
                     /locus_tag="ACHA6B_002954"
     CDS             complement(<1..886)
                     /locus_tag="ACHA6B_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8950416.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPS"
BASE COUNT          302 a          106 c          161 g          327 t
ORIGIN      
        1 ttgaagggga taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa
       61 atcctaatat atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg
      121 ctttttccat ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa
      181 aatgtttttt taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa
      241 aattttctga aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat
      301 aaagaatatg ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa
      361 gttcctgttg ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa
      421 ctaataagga aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt
      481 cctgatcata ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat
      541 ttttcagaaa agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa
      601 tatgtgctaa taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct
      661 tttgagtaga aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat
      721 ctagttctat agatttatgt gcaaattcag catataaatt agcgctttca tttattagta
      781 gtcgaaaata gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt
      841 ttttgacaag attggaggat tggttgtttg ttaacaaaag aaccactttc ctttct
//
LOCUS       DAYYHB010000254          857 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000254 DAYYHB010000000
VERSION     DAYYHB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.266"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>857)
                     /locus_tag="ACHA6B_002955"
     CDS             complement(<1..>857)
                     /locus_tag="ACHA6B_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950417.1"
                     /translation="DFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEAN
                     IRSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAI
                     NIERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVI
                     DGNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERS
                     YLFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLD
                     YYYVLDYYD"
BASE COUNT          309 a          144 c          108 g          296 t
ORIGIN      
        1 aaatcataat aatctaacac ataataataa tccaaaaata attgtgccca gttttctaat
       61 ttctttgata catccaaatc cgcatttgct tgtaattctt ttttgagttt ttccattatg
      121 tgataagttc gaggttcttc ttctttcatt tcttcgatat acgaacgatt aaaaaaaaga
      181 tctggattac cataaaatag ataacttctt tcatgaaatg caataaaatt gaatatgagt
      241 tctggatcct ttatatctaa agaaaaacgg aacatgcttt gtaaaacttt ctccaaattc
      301 tcaactgctc gataagaaca agtattttgt cgctgatgtg catatgcatg actattttca
      361 tatattgcat ttccatcaat tactccaaaa gaataaatta ttcgataaag gaaacacagt
      421 tcatcgctgt taaaagaaag attgatttgt tttgaccact tcttcaaaag attaaaatgc
      481 ggatcatctg agataactga tttatattca ttatctactc gtacttttct aaatgaactt
      541 ctttctatat taatggctaa ccaaaaagaa aatacacgtt tttgaaaaat agttaacgta
      601 atatcataaa tcccctcaaa agcaactata tacttctcta tttccttttg ttcgatatga
      661 aatggccact tatcactact ttcacgtgta gaattccaat agaaataata aaaaaagcta
      721 cgaatgtttg cctctgatgc attcaattct aactgaaatg gatgaaatgt tatatgataa
      781 cgcctaaaat atggaattaa gagcttcgca tatctgaaaa cagtagatac actagtaaat
      841 tgctctttag caaaatc
//
LOCUS       DAYYHB010000255          834 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000255 DAYYHB010000000
VERSION     DAYYHB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.267"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>834
                     /locus_tag="ACHA6B_002956"
     CDS             25..>834
                     /locus_tag="ACHA6B_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8950418.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPK"
BASE COUNT          293 a          157 c          154 g          230 t
ORIGIN      
        1 gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga cgaactgaca
       61 atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa ccgaatggga
      121 cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa gactgctctt
      181 gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt cattcaatta
      241 cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac gcctgacgtc
      301 attatcgggc gaaaagaaag gcctgtttca tgcggtatga gaacacttta tcgtttattt
      361 tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa acccaatggc
      421 catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg acctgataat
      481 tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt tggcattcat
      541 cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat cacgattaaa
      601 cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt ttctcgcttc
      661 cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc taactggaaa
      721 gccattagta accaacatga tattgatata tattttgcgg accctggaac accttctcaa
      781 cgcccattaa acgagaattc taacgggatt ctgcgtcgta atggactgcc gaaa
//
LOCUS       DAYYHB010000256          826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000256 DAYYHB010000000
VERSION     DAYYHB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.268"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHA6B_002957"
     CDS             136..309
                     /locus_tag="ACHA6B_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950419.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          277 a          135 c          104 g          310 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actcca
//
LOCUS       DAYYHB010000257          780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000257 DAYYHB010000000
VERSION     DAYYHB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.269"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>780
                     /locus_tag="ACHA6B_002958"
     CDS             <1..>780
                     /locus_tag="ACHA6B_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8950420.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          277 a          152 c          140 g          211 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
//
LOCUS       DAYYHB010000258          774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000258 DAYYHB010000000
VERSION     DAYYHB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.270"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..495)
                     /gene="spxA"
                     /locus_tag="ACHA6B_002959"
     CDS             complement(97..495)
                     /gene="spxA"
                     /locus_tag="ACHA6B_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8950421.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          253 a          139 c          106 g          276 t
ORIGIN      
        1 tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc
       61 tttgtaatag tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc
      121 ttgacgtaat tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt
      181 aaaaccaact tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt
      241 gttttgcaca agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt
      301 ttggaatact ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa
      361 aatcgctttt aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg
      421 aatctgatgt tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt
      481 atataatgtt aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag
      541 aagatcataa taatctccca tctgtttcta ttatacataa tcaactttta aatagctacc
      601 tttttttcat aattttttta aaaaaatgaa agttatctaa ggttaatata cagtaaaacc
      661 agcattctcg ctttttttat catttaaaaa ttagagtttt caaatctaat tcaaattttc
      721 taaacatatg aaacagcgta taaaactgta ctattatttt atctcaaaag ctaa
//
LOCUS       DAYYHB010000259          771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000259 DAYYHB010000000
VERSION     DAYYHB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.271"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>771)
                     /locus_tag="ACHA6B_002960"
     CDS             complement(<1..>771)
                     /locus_tag="ACHA6B_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8950422.1"
                     /translation="FIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTS
                     IILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANS
                     KFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNES
                     DKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFL
                     KKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICI"
BASE COUNT          288 a          134 c           98 g          251 t
ORIGIN      
        1 gatacatata aaagcaatta taccacttga tacaagtact tgaagaaatc cattatgcat
       61 gtttccatgt gctcttttta gttcattcac atctgtagta tcaagatcta cttttttcaa
      121 aaattttgca tcgctattcc catagaaatc ggtctctcca taaccaaata acaaagttgg
      181 tcttataact ttaagcgcag ctttccaaat cgtaaaacga ccagctgaaa tttcggagtt
      241 atcactagaa tgctgtaata ttacttcttt ttctggaagt ggagttttat ctgattcatt
      301 tgactctata acttctactt ttctatcaat cgatacagga attgttgcta aaaaatcctt
      361 agcataatta gtaccaaaag aaaaatatac caatagaact aataatatgc ttaccatcca
      421 tttgattttt acaggtagta cttctctaat gctctttcta aattttgaat tggcaaacaa
      481 caaaagaaat aatactaaaa aagaacaaat tactacctgg gcacctctag acgaagaaag
      541 tgtaatataa aatatttgta cgataaaatt catcatataa aaaacacgaa tcgtttttgt
      601 tatttttgaa attaatacac taattaaact taaaataata gaagtatatc caaacatagc
      661 tccaacattt ggacttgtat agacaccaaa taaccgatgt tcaataaaac cttgtcttgc
      721 aagtaatcct ggcgttcctg ttgggacatg ataagaaatc aaaaaaataa a
//
LOCUS       DAYYHB010000260          765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000260 DAYYHB010000000
VERSION     DAYYHB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.272"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            660..>765
                     /locus_tag="ACHA6B_002961"
     rRNA            660..>765
                     /locus_tag="ACHA6B_002961"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          315 a          124 c          134 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaaga
//
LOCUS       DAYYHB010000261          723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000261 DAYYHB010000000
VERSION     DAYYHB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.273"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..>723
                     /locus_tag="ACHA6B_002962"
     CDS             12..>723
                     /locus_tag="ACHA6B_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950423.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDI"
BASE COUNT          242 a          120 c          118 g          243 t
ORIGIN      
        1 ggaatacctt tatgtctttt tttaataaca tatataataa aaaagaatct ttttatttac
       61 gtataaagtg gagcataatc attatattat cattgttatt tttaacagga attgcttatt
      121 tcttttattt tattttttct cctgcggaca gcaatcaaat gaaagaaata aaagattcgt
      181 gggttttcta tacaaaagat gatcctgatt ttaagtttga cagcaagtac actcgtacga
      241 ttcctaccgt ggataaagac gaaacattca ttatggaaac gacacttgaa aaaccgttgg
      301 acgaagcaaa tttactaatc aaaggaaacc atcaatggat tactgtatat ctaggcgaaa
      361 agattttata ccaacggaaa gattatcagg aagaaagcaa tcccggactc tcattggctg
      421 tgatagattt accagcaaat tatatcggaa aaaaattgac ccttacagtc tcatctcctt
      481 atcagaacta tgctggactg ccaccgaaag tttatctagg aacaactgga ccattgatta
      541 gttttattta ttcacaatca gtgccgcaag tattgacgat gataatcgct gtttttatcg
      601 ctatagggac ctttttctat acgatctact tgatgtataa acaaaaacgt cttgaatact
      661 cacttattct cttaagttgc tttgctttgg cattaggatt tgaagctgta tcagaagata
      721 ttt
//
LOCUS       DAYYHB010000262          709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000262 DAYYHB010000000
VERSION     DAYYHB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.274"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACHA6B_002963"
     CDS             <1..600
                     /locus_tag="ACHA6B_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8950424.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAYYHB010000263          697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000263 DAYYHB010000000
VERSION     DAYYHB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.275"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>697
                     /locus_tag="ACHA6B_002964"
     CDS             <1..>697
                     /locus_tag="ACHA6B_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950425.1"
                     /translation="FIDEKGRLVDEDGNLIDTNGKPLEDSEDGNIGMDSEGRLIDEKG
                     RLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLVDEDGNLVDEFGNKLGKHSDG
                     YNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIAVAIPENEEDEITLDQTHQEL
                     RNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSAIRNDTVLKDRESLTKETDKE
                     QSDVKKKFIPTPNV"
BASE COUNT          248 a           87 c          185 g          177 t
ORIGIN      
        1 ctttattgac gaaaaagggc gtttggtcga tgaagatgga aaccttatag atactaatgg
       61 taaaccattg gaagatagtg aagatggtaa tattggtatg gatagcgaag gacgtttaat
      121 tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt gttgacgaaa atggacgcag
      181 gttgctaaat ggtgattttg gtcatgatag taacggacgt ttagttgacg aaaaaggtcg
      241 tttagttgat gaagatggaa atcttgttga tgagtttggt aataaacttg gtaaacacag
      301 cgatggatat aatgtgaatg gagatttagt cgatgaggat gggaatatca ttgagacgag
      361 agatgcgctt gtggcagatc gaaatagaga actcgaacaa caacggcata cgccaattgc
      421 tgttgcaatt cctgagaatg aagaagacga gattacttta gatcaaactc atcaagaact
      481 gaggaatgaa atgagtggtg aggacgtgtc agacagagtg catcgagaat taggagatgg
      541 agcatctggt gaaagtcagt caagagagct taacagcaac tttgataata gtgctattcg
      601 gaatgacaca gtacttaaag acagagaatc gctaacaaaa gaaacggata aagaacaatc
      661 agatgtgaag aaaaagttca ttcctactcc taatgtt
//
LOCUS       DAYYHB010000264          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000264 DAYYHB010000000
VERSION     DAYYHB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.276"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..628)
                     /locus_tag="ACHA6B_002965"
     CDS             complement(53..628)
                     /locus_tag="ACHA6B_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8950426.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DAYYHB010000265          658 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000265 DAYYHB010000000
VERSION     DAYYHB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.277"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..316
                     /locus_tag="ACHA6B_002966"
     CDS             <1..316
                     /locus_tag="ACHA6B_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8950427.1"
                     /translation="LTIPGAMSPTEIFKMFEMGADIVKVFPANCLGPEYFNQVQAPLG
                     SLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKSAVHQLNYERLMELAESFIDSLR
                     VE"
BASE COUNT          218 a          111 c          132 g          197 t
ORIGIN      
        1 tctaacaatt cctggggcca tgtcgcccac agagatattc aaaatgtttg agatgggtgc
       61 tgatattgtt aaggtgttcc ccgccaactg tcttggaccg gaatatttca atcaagtgca
      121 agccccttta ggaagtttac ctttgatggc tgtaggaggg gtagatcaga ctaatgcaca
      181 aaactatttg aacaatggag cctcttatgt aggaattggt tcaaaatttt ttgaaaaaag
      241 tgctgttcac caactaaatt acgagaggct aatggagttg gctgaatctt ttatagatag
      301 tttaagagtt gaatagaacc catttgcgga atacaacttg ttttataata tggcgttatc
      361 tgtataaaac atagtatcac agtgaatgag attgaactgg tagattgatt tttcgaccag
      421 ttctttttgc gcgctctgat gcttgatata ttcccaacga gataaaaaat cccccaagat
      481 actaagatga agcgtttatc caacctcata cttagataaa aaggaggaaa tgttctgaat
      541 gataaacaaa aattagcgcg cacaatttgg cgatgaaaaa atcatattgt atttacgtca
      601 aaatatcacc atcaaattat ttatggaaaa cataataggc ataaacccga ttgctcgg
//
LOCUS       DAYYHB010000266          639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000266 DAYYHB010000000
VERSION     DAYYHB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.278"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACHA6B_002967"
     CDS             <1..282
                     /locus_tag="ACHA6B_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8950428.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatt
//
LOCUS       DAYYHB010000267          627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000267 DAYYHB010000000
VERSION     DAYYHB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.279"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..558
                     /locus_tag="ACHA6B_002968"
     CDS             <1..558
                     /locus_tag="ACHA6B_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950429.1"
                     /translation="FTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNASVV
                     INGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTK
                     GTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENS
                     DITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT          244 a          103 c          101 g          179 t
ORIGIN      
        1 ttcacaactt cacacaataa aagatattct ctccttttcc agtggctata tttgactggg
       61 atgaggtctg gtgaagcaat cgcattaagt aaagatgatg tacacattac caataataat
      121 gcatcagtag ttataaatgg gacgatgatg tatagagaac gttcaatagc tgatatgaaa
      181 aaatctgatt ctacaaaaac agctgccgga atgcgagaaa ttgatttacc aaaaaaagcg
      241 atagctattt acaatgagct tctagaatta aatccaaatg gtcaatttct atttcaaaca
      301 acgaaaggaa ctcctttcca actaacagca attaacacct atttgagaag tcataaagct
      361 gacatgaaga ttgataaaaa acttagttca catattttta gacataccca tatttcaaaa
      421 ttagcggaac taggaactcc tctgtatgcc attcaggatc gcgttggtca cgaaaatagt
      481 gatatcactg aaaaaattta tttgcatgtg acaaaaggag taaaagaaaa attgaaagaa
      541 gatatagaaa aactgtaact tgttgcccct tttttgcccc tttttattaa tcaaaaatgt
      601 accaaataga aatcgttaat ataaaaa
//
LOCUS       DAYYHB010000268          596 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000268 DAYYHB010000000
VERSION     DAYYHB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.280"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..228
                     /gene="relB"
                     /locus_tag="ACHA6B_002969"
     CDS             7..228
                     /gene="relB"
                     /locus_tag="ACHA6B_002969"
                     /inference="COORDINATES: protein motif:HMM:NF039402.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8950430.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            230..496
                     /locus_tag="ACHA6B_002970"
     CDS             230..496
                     /locus_tag="ACHA6B_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8950431.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
BASE COUNT          223 a           97 c          115 g          161 t
ORIGIN      
        1 taaactatgc cagtaataag tattcgtgta tctaaagaag aaaaagattt tcttgaaaaa
       61 gttgctgaat tcaacggaga taacatttct gaatttatgc gtagtaattc cctgcaaaca
      121 gcagaaacgt tagtagactt tgccacctac aaggaactaa tgaaagagca tgaggaaaac
      181 gatcaaagta tttcccacga agatatgcta aaagagttag gcttataaga tgacttacca
      241 agtaagatat gaacgtgaag ctcaaaaagc gctaaaaaaa atggataagt ttcaggcaaa
      301 ggttatccta aactggatcg agaaaaatct tgtaggcact gatgatccaa gaaaacatgg
      361 taaaggtcta acagctaata aatcaggcta ctggcgatat agggtaggtg cctaccgtat
      421 aatagctgat attagcgagc atgaagtgac aattcttata ttgtctattg gtcacagaag
      481 tgaagtttat aagtaacaga caagctgata aggcttgtct gtttttttgt taacccaaca
      541 aaatcactgt ttacgatccc actttaccta aaaaagaaca ataccctctt gttatt
//
LOCUS       DAYYHB010000269          591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000269 DAYYHB010000000
VERSION     DAYYHB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.281"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>591
                     /locus_tag="ACHA6B_002971"
     CDS             26..>591
                     /locus_tag="ACHA6B_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8950432.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAYYHB010000270          589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000270 DAYYHB010000000
VERSION     DAYYHB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.282"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACHA6B_002972"
     CDS             complement(<1..434)
                     /locus_tag="ACHA6B_002972"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8950433.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT          166 a          128 c           98 g          197 t
ORIGIN      
        1 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
       61 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
      121 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
      181 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
      241 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
      301 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
      361 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
      421 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
      481 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
      541 catggtaaac taaatacgtt agtttacaaa aagaatacct gttctttta
//
LOCUS       DAYYHB010000271          581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000271 DAYYHB010000000
VERSION     DAYYHB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.283"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..351)
                     /locus_tag="ACHA6B_002973"
     CDS             complement(<1..351)
                     /locus_tag="ACHA6B_002973"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8950434.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKD"
     gene            430..>581
                     /locus_tag="ACHA6B_002974"
     CDS             430..>581
                     /locus_tag="ACHA6B_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8950435.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          211 a           86 c           79 g          205 t
ORIGIN      
        1 atctttataa tcatttttac cacctaattt ttcatataca tggcaagctc taggattacc
       61 tttatcagtt attaaaaaca tttcagaaca accaatctct ttagaatatt ccttaataaa
      121 agataataat tttgaaccat aacctttgtc ttgatagtta ggtaacattc ctattgagtg
      181 taaataaaac attgtttttc catcaggtct taaaagtgta tagcaatatg caaatcctat
      241 aattttatta ttttctttag ctataaaccc aaatgaactg gtatcattaa gaaatccttt
      301 taaattatca atatcaaaaa ccatattatc atcaataaac aatagtttca ataggaggtt
      361 ctgttgcaaa gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag
      421 gacagagtaa tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct
      481 gttggttact acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat
      541 cgtggaataa atgtttgtca tactacgatt tatcgttggg t
//
LOCUS       DAYYHB010000272          576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000272 DAYYHB010000000
VERSION     DAYYHB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.284"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..446)
                     /locus_tag="ACHA6B_002975"
     CDS             complement(15..446)
                     /locus_tag="ACHA6B_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFM8950436.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          205 a           73 c           65 g          233 t
ORIGIN      
        1 aattaaataa atttttagtt attaacttga ttaaaatcag agataatatc atttaattca
       61 gtttgaaggg ttgtaacgat taaatggaaa ttttcttcat ctgttttatt atatggggat
      121 gttttcgtta tatataacaa cttttctttt tcaattttca ttaataactc tgtcgaagtc
      181 tgtactttat cttgataatt aaatgcagac ataccacttt gtataattgt aattattgct
      241 gaagcaattg aaacaatcaa tagtaaagac ctgtgttcgg aagcctgatc aattaatatt
      301 gggatactag ctgataagac tatttttata atatttccga taccaattac tctattataa
      361 aatcgaattt ctttttttaa tctatcgatt gtagaatcaa tagattcgat aaacgctttt
      421 tgatccatca ttttctctcc aatcatttta ttaaaatcat taacaaaaat attataatag
      481 gatttaagta agttctaact aatcacatat aaaaataaaa aaacatttgt attttatcaa
      541 agttttatta tacttgtcaa tttgtgtaca tcctaa
//
LOCUS       DAYYHB010000273          557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000273 DAYYHB010000000
VERSION     DAYYHB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.285"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..549
                     /locus_tag="ACHA6B_002976"
     CDS             <1..549
                     /locus_tag="ACHA6B_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950437.1"
                     /translation="KSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGT
                     YDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPIS
                     IQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLK
                     IYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          235 a           81 c           69 g          172 t
ORIGIN      
        1 aaaagttcac atcaacagtc acgttattct ttaattacta aatttttgtt tttaactgga
       61 ctacgttatg gtgaattgat atctttgact gaatctgatt atgatggtaa taaaattacg
      121 gtttctggta cttatgatta cgagttaaaa attaaaacaa caactaaaaa tacaggttct
      181 tatagaacca ttgaattatc aagtaatgct aaagaaatca tagatcaatt aattgaagaa
      241 aacaaattaa ttaagaataa taaagataaa tacattttca tctctaaaaa tggtaacccg
      301 atatctattc aagcatataa tcaatcttta agagcagtat ccaaagaatt aaacatagat
      361 aaaaaagttt cttctcacat gttaaggcat tcccacattt ctctacttac agaattagga
      421 atcccattaa aagccattat ggatcgtgta ggacatgaag attcaaaaac aacattaaag
      481 atttatactc atacaactaa aaatatgcaa aatcaactag tagaaaaact aggaaaaata
      541 gaaatttaaa ttgcccc
//
LOCUS       DAYYHB010000274          536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000274 DAYYHB010000000
VERSION     DAYYHB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.287"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>536)
                     /locus_tag="ACHA6B_002977"
     CDS             complement(44..>536)
                     /locus_tag="ACHA6B_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8950438.1"
                     /translation="RVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          155 a          130 c           92 g          159 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcga
//
LOCUS       DAYYHB010000275          471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000275 DAYYHB010000000
VERSION     DAYYHB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.288"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..>471
                     /locus_tag="ACHA6B_002978"
     CDS             68..>471
                     /locus_tag="ACHA6B_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950439.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQR"
BASE COUNT          212 a           68 c          101 g           90 t
ORIGIN      
        1 ttataatcta gaaacatttt gaataaaaac tattagacaa atgatatttt acgaggagga
       61 aacggtcatg gcagaagaaa aagaaccact agacaatagt cgtttgggaa aatccaaaag
      121 aaaattagta cgattgcaaa atgaattaaa cgaacaaatt gaaaagatgt ttgaacacca
      181 gagaaagacc aacggtcaac caatgaacga taagagaaat gggcattctt ggtttaggca
      241 acaagagcga atcgaaaata aagtgcatag tctgagagaa gagatcaagc aacaagaaaa
      301 acaagtagaa aaacttgaaa gacaagaaga gataaaggaa atgggctata acaagtacgg
      361 cggactggat atgacgatcg agaatattcc aagaatcaaa gaagaaatcg aacgctttga
      421 aaaaggtgaa tcgacttttt cagccgctac gatccgaaaa taccaacgga a
//
LOCUS       DAYYHB010000276          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000276 DAYYHB010000000
VERSION     DAYYHB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.289"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..>468
                     /locus_tag="ACHA6B_002979"
     CDS             65..>468
                     /locus_tag="ACHA6B_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950440.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQR"
BASE COUNT          208 a           70 c          102 g           88 t
ORIGIN      
        1 taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg aggaggaaac
       61 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
      121 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      181 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      241 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      301 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      361 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      421 aggtgaatcg actttttcag ccgccacgat ccgaaaatat caacggaa
//
LOCUS       DAYYHB010000277          463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000277 DAYYHB010000000
VERSION     DAYYHB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.290"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>463
                     /locus_tag="ACHA6B_002980"
     CDS             <1..>463
                     /locus_tag="ACHA6B_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8950441.1"
                     /translation="RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSC
                     GEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT          148 a           78 c           92 g          145 t
ORIGIN      
        1 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
       61 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      121 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      181 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      241 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      301 ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag tagaatttgt
      361 ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg actatgtcaa
      421 ttggtggaac caccttcggt tgcacggtac acttggctac gag
//
LOCUS       DAYYHB010000278          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000278 DAYYHB010000000
VERSION     DAYYHB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.291"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..398)
                     /locus_tag="ACHA6B_002981"
     CDS             complement(<1..398)
                     /locus_tag="ACHA6B_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950442.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGE"
BASE COUNT          167 a           65 c           77 g          151 t
ORIGIN      
        1 ttttcaccag gtaatatatc tttcatatct aaaacttctg aaaaagcgac tgagtagcca
       61 ggagagattg cttgtgccaa cggtatagga gataaaaggc tacttttatg aatgcttccc
      121 atatcaaaac cattggcgtg ataatgtgaa caattaattt tcttaccgtt gtaaagatac
      181 gtacattcga taattgagtt tggcattttt gatgggtttg caattacgcc agtaagaatc
      241 attcgattac ttctatcatg cttaactaaa aaataggaga gcgtaatttt tcttttatct
      301 ttaaataagt tatagaggga taaaaaaaat cctgaaatag aaatgattaa agtaatagcg
      361 acttggtaat cgctcaaaat ttttatcaag taattcacca cctttttact caaattatat
      421 caaaaaggag agaagaaata atgcaagaat tagtaatttt
//
LOCUS       DAYYHB010000279          458 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000279 DAYYHB010000000
VERSION     DAYYHB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.292"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACHA6B_002982"
     CDS             143..400
                     /locus_tag="ACHA6B_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950443.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          198 a           60 c           64 g          136 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttagg
//
LOCUS       DAYYHB010000280          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000280 DAYYHB010000000
VERSION     DAYYHB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.293"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..272)
                     /locus_tag="ACHA6B_002983"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="ACHA6B_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          148 a           75 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DAYYHB010000281          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000281 DAYYHB010000000
VERSION     DAYYHB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.294"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHA6B_002984"
     CDS             <1..>451
                     /locus_tag="ACHA6B_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950444.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYHB010000282          440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000282 DAYYHB010000000
VERSION     DAYYHB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.295"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>440
                     /locus_tag="ACHA6B_002985"
     CDS             11..>440
                     /locus_tag="ACHA6B_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8950445.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DAYYHB010000283          435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000283 DAYYHB010000000
VERSION     DAYYHB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.296"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          161 a           46 c           84 g          144 t
ORIGIN      
        1 ttttttgaaa ctatctatag atatatttac tggaattttt ggaggtatag aaacagttat
       61 cattttatct ttgtaccacc aatgatttat atcagctcaa cagagtgaat tgtatttacg
      121 caagaatgcg taggatgaaa tggatagata attaagatat atatgtatat ggtgactgaa
      181 gacaaaaaaa cagaactaat gaaactgaat aaaaatatag agtgaactag tttctaagag
      241 ggattttaaa ctagatttca ttggtaataa aatttggaca gatggaaaaa catcacataa
      301 ggaagcagat tttgttgtca tttttgttcc tacaaactat ggtagtgaat gtgttattga
      361 attagtgatg tctgttactt cagaggcatt ggggataatt aaatccacta tacctgtagg
      421 atatacaaaa agtgg
//
LOCUS       DAYYHB010000284          434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000284 DAYYHB010000000
VERSION     DAYYHB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.297"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..224
                     /locus_tag="ACHA6B_002986"
     CDS             39..224
                     /locus_tag="ACHA6B_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM8950446.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            291..>434
                     /locus_tag="ACHA6B_002987"
     CDS             291..>434
                     /locus_tag="ACHA6B_002987"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8950447.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALG"
BASE COUNT          155 a           76 c           88 g          115 t
ORIGIN      
        1 acaatgtgta taatagattt tgtaaaggga gggtagctat gcctaagaca gcaagagaaa
       61 ttgtaaaaat tctcaaatct aacggcttca aaaagactgg acaaactggc agtcatgcaa
      121 aattttataa ttccaagact aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac
      181 cactaggaac agaagctaat atctggaagc aagcagggct ttaacagcct tgccttccta
      241 tttggtatta ggcatactat cctctcttac actagaaaag gagcgaggaa atgaagacaa
      301 aaaatatttt ttatccggca atcttcgaga aagaaggaga agcttataac atcgttttcc
      361 ctgacttacc ggaaatatct acctttggaa atagtcttga agaagcatat ttaaacgcta
      421 aggatgcttt agga
//
LOCUS       DAYYHB010000285          430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000285 DAYYHB010000000
VERSION     DAYYHB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.298"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>430
                     /locus_tag="ACHA6B_002988"
     CDS             <1..>430
                     /locus_tag="ACHA6B_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8950448.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DAYYHB010000286          424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000286 DAYYHB010000000
VERSION     DAYYHB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.299"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          132 a           87 c           69 g          136 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttc
//
LOCUS       DAYYHB010000287          418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000287 DAYYHB010000000
VERSION     DAYYHB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.300"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHA6B_002989"
     CDS             <1..387
                     /locus_tag="ACHA6B_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950449.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          160 a           74 c           65 g          119 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttattta
//
LOCUS       DAYYHB010000288          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000288 DAYYHB010000000
VERSION     DAYYHB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.301"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHA6B_002990"
     CDS             <1..365
                     /locus_tag="ACHA6B_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950450.1"
                     /translation="DPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKE
                     LVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAET
                     LDQLLPWNKDVQQHFSRN"
BASE COUNT          135 a           75 c           84 g          121 t
ORIGIN      
        1 tagatcctgt tgcaaaatcc aagttggatg cagccgttca atatgcgtgt aaactacgaa
       61 gtggttttga acctattttt gaagatggtc gtttagaact cacaaataat cgtgcggagc
      121 gaaatatcaa agagctggtg ataggacgga aaaattggtt gcattccaca agtcttgaag
      181 gagcacgaac ttcaggaatc attctgagtg tatataaaac agcagaactt aacggattga
      241 atcctgtaaa gtatctagag tttctatttg ataagatacc aaatcttccc gtactttcag
      301 cagaaacttt agatcaactg ttgccttgga acaaggatgt acaacaacat ttttctcgta
      361 actaaaaacg ccaaacattc ccgattctat tctataggtc gggttatttg acgta
//
LOCUS       DAYYHB010000289          406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000289 DAYYHB010000000
VERSION     DAYYHB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.302"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..406
                     /locus_tag="ACHA6B_002991"
                     /pseudo
     CDS             1..406
                     /locus_tag="ACHA6B_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          170 a           68 c           76 g           92 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtac
//
LOCUS       DAYYHB010000290          399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000290 DAYYHB010000000
VERSION     DAYYHB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.303"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAYYHB010000291          365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000291 DAYYHB010000000
VERSION     DAYYHB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.304"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           52 c           54 g          133 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attac
//
LOCUS       DAYYHB010000292          351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000292 DAYYHB010000000
VERSION     DAYYHB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.305"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>351
                     /locus_tag="ACHA6B_002992"
     CDS             <1..>351
                     /locus_tag="ACHA6B_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFM8950451.1"
                     /translation="LDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFR
                     KLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYK
                     KNRRNGTLFGFSVST"
BASE COUNT          118 a           72 c           71 g           90 t
ORIGIN      
        1 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
       61 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      121 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      181 aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt
      241 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      301 tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac t
//
LOCUS       DAYYHB010000293          348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000293 DAYYHB010000000
VERSION     DAYYHB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.306"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA6B_002993"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA6B_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8950452.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          110 a           52 c           56 g          130 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaaca
//
LOCUS       DAYYHB010000294          340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000294 DAYYHB010000000
VERSION     DAYYHB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.307"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..164
                     /locus_tag="ACHA6B_002994"
     CDS             42..164
                     /locus_tag="ACHA6B_002994"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950453.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            137..268
                     /locus_tag="ACHA6B_002995"
     CDS             137..268
                     /locus_tag="ACHA6B_002995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950454.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          122 a           46 c           64 g          108 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc
//
LOCUS       DAYYHB010000295          333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000295 DAYYHB010000000
VERSION     DAYYHB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.308"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>333
                     /locus_tag="ACHA6B_002996"
     CDS             <1..>333
                     /locus_tag="ACHA6B_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950455.1"
                     /translation="KINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQANLLLN
                     EKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDDVLVK
                     NVNSNLIQ"
BASE COUNT          152 a           49 c           46 g           86 t
ORIGIN      
        1 aaaaaataaa tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa
       61 aaaagagaaa agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta
      121 acttactttt gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa
      181 cttttggaga attaaaaact aaatgggatg aaaaatacaa gccaactgta aaagaaagtt
      241 cctatcgaac aactcaggtt tatctatcat taatttcaaa atatataaaa gatgatgtac
      301 ttgtaaaaaa cgttaatagt aatcttatac aag
//
LOCUS       DAYYHB010000296          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000296 DAYYHB010000000
VERSION     DAYYHB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.309"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..242
                     /locus_tag="ACHA6B_002997"
     CDS             48..242
                     /locus_tag="ACHA6B_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950456.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          144 a           44 c           60 g           73 t
ORIGIN      
        1 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
       61 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      121 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      181 agaacgagct agaaaaagaa ctgacttgtg gcaaagctgt tgtagatatc atcgaaaagt
      241 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      301 atagaattag aagaagaaga c
//
LOCUS       DAYYHB010000297          318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000297 DAYYHB010000000
VERSION     DAYYHB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.310"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..>318
                     /locus_tag="ACHA6B_002998"
     CDS             74..>318
                     /locus_tag="ACHA6B_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950457.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVV"
BASE COUNT          122 a           45 c           39 g          112 t
ORIGIN      
        1 aacgcaaaac ttattaaaaa aagacaattc tagtatactg aaaatagaat taaaaaagga
       61 gaacaacaaa tctatgaaaa taagtaaaaa agcatttctt ttagtagttc ttatcttact
      121 tagtacttta tattcagtaa attttatgag aaatgctcaa gaaatttata caacaggtga
      181 tttatctttt catctttcac gaatcaaagg gttatcttct atttttgaag ggccaatcaa
      241 ctatacaaca tttaacaatt atggagatgg actgaactat ttttatcctt ttcttacaat
      301 aattcctgca gtcgtttt
//
LOCUS       DAYYHB010000298          311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000298 DAYYHB010000000
VERSION     DAYYHB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.311"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..>311
                     /locus_tag="ACHA6B_002999"
     CDS             69..>311
                     /locus_tag="ACHA6B_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8950458.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          100 a           58 c           67 g           86 t
ORIGIN      
        1 aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa ataaagggag
       61 aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg tgtgccattg
      121 gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa
      181 caatgccttt agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga
      241 ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct
      301 cgaccccgaa a
//
LOCUS       DAYYHB010000299          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000299 DAYYHB010000000
VERSION     DAYYHB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.312"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>308
                     /locus_tag="ACHA6B_003000"
     CDS             <1..>308
                     /locus_tag="ACHA6B_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8950459.1"
                     /translation="TPDVIIGRAERTFSCSVSTLYRRFKTGEFNVLHLPMQGKRKPNG
                     YKEKRGKQAFKRNISERKKDYVVFEEEFGHLEGDTIVGIHHKSAVITLVERLSKAIIA
                     "
BASE COUNT          102 a           62 c           65 g           79 t
ORIGIN      
        1 gaccccagac gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct
       61 ctatcgccgg tttaagacgg gagaattcaa tgttttacat ttaccgatgc aaggaaaacg
      121 aaaaccaaat ggttataagg aaaaacgtgg aaaacaggca ttcaaaagaa atatttctga
      181 acgtaaaaaa gattatgtcg ttttcgaaga agaatttgga catttagagg gtgatacgat
      241 tgtcggcatc caccataaaa gtgccgtcat cacactcgtt gagcgacttt caaaagccat
      301 tatcgcct
//
LOCUS       DAYYHB010000300          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000300 DAYYHB010000000
VERSION     DAYYHB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.313"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>300
                     /locus_tag="ACHA6B_003001"
     CDS             <1..>300
                     /locus_tag="ACHA6B_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8950460.1"
                     /translation="NKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYASR
                     LDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          103 a           34 c           56 g          107 t
ORIGIN      
        1 tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta ctaataggga
       61 tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg gatgacgtag
      121 tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa cattataaga
      181 catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa cactttatgc
      241 tatggaaatt attaaattct acgattatgt taggaattgt agtattactt tgcaagtatg
//
LOCUS       DAYYHB010000301          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000301 DAYYHB010000000
VERSION     DAYYHB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.314"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHA6B_003002"
     CDS             <1..>270
                     /locus_tag="ACHA6B_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8950461.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYHB010000302          265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000302 DAYYHB010000000
VERSION     DAYYHB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.denovo.315"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>265
                     /locus_tag="ACHA6B_003003"
     CDS             <1..>265
                     /locus_tag="ACHA6B_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8950462.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAYYHB010000303        28016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.030, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000303 DAYYHB010000000
VERSION     DAYYHB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.030"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..165
                     /locus_tag="ACHA6B_003004"
     CDS             <1..165
                     /locus_tag="ACHA6B_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8950463.1"
                     /translation="GIGQTELIRDLQLRKIAITRETLVKIEGGRQHIKLDQLKAIKDI
                     LQVSYEDLLQ"
     gene            321..1172
                     /locus_tag="ACHA6B_003005"
     CDS             321..1172
                     /locus_tag="ACHA6B_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8950464.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            1205..1882
                     /locus_tag="ACHA6B_003006"
     CDS             1205..1882
                     /locus_tag="ACHA6B_003006"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8950465.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            2370..3335
                     /locus_tag="ACHA6B_003007"
     CDS             2370..3335
                     /locus_tag="ACHA6B_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8950466.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3357..3569
                     /locus_tag="ACHA6B_003008"
     CDS             3357..3569
                     /locus_tag="ACHA6B_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8950467.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3644..4120
                     /locus_tag="ACHA6B_003009"
     CDS             3644..4120
                     /locus_tag="ACHA6B_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8950468.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4117..5925
                     /locus_tag="ACHA6B_003010"
     CDS             4117..5925
                     /locus_tag="ACHA6B_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8950469.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6002..6217
                     /locus_tag="ACHA6B_003011"
     CDS             6002..6217
                     /locus_tag="ACHA6B_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8950470.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6309..7172
                     /locus_tag="ACHA6B_003012"
     CDS             6309..7172
                     /locus_tag="ACHA6B_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8950471.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            7209..7745
                     /locus_tag="ACHA6B_003013"
     CDS             7209..7745
                     /locus_tag="ACHA6B_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8950472.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            7759..8181
                     /locus_tag="ACHA6B_003014"
     CDS             7759..8181
                     /locus_tag="ACHA6B_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8950473.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8111..10510
                     /locus_tag="ACHA6B_003015"
     CDS             8111..10510
                     /locus_tag="ACHA6B_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8950474.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10542..12533
                     /locus_tag="ACHA6B_003016"
     CDS             10542..12533
                     /locus_tag="ACHA6B_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8950475.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12556..12807
                     /locus_tag="ACHA6B_003017"
     CDS             12556..12807
                     /locus_tag="ACHA6B_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8950476.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12797..14026
                     /locus_tag="ACHA6B_003018"
     CDS             12797..14026
                     /locus_tag="ACHA6B_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8950477.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14023..16104
                     /locus_tag="ACHA6B_003019"
     CDS             14023..16104
                     /locus_tag="ACHA6B_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8950478.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16253..20173
                     /locus_tag="ACHA6B_003020"
     CDS             16253..20173
                     /locus_tag="ACHA6B_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8950479.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            20174..21118
                     /locus_tag="ACHA6B_003021"
     CDS             20174..21118
                     /locus_tag="ACHA6B_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950480.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21122..21325
                     /locus_tag="ACHA6B_003022"
     CDS             21122..21325
                     /locus_tag="ACHA6B_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8950481.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21625..21861)
                     /locus_tag="ACHA6B_003023"
     CDS             complement(21625..21861)
                     /locus_tag="ACHA6B_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950482.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22044..22415)
                     /locus_tag="ACHA6B_003024"
     CDS             complement(22044..22415)
                     /locus_tag="ACHA6B_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8950483.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(22412..23740)
                     /locus_tag="ACHA6B_003025"
     CDS             complement(22412..23740)
                     /locus_tag="ACHA6B_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8950484.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23701..24030)
                     /gene="mobC"
                     /locus_tag="ACHA6B_003026"
     CDS             complement(23701..24030)
                     /gene="mobC"
                     /locus_tag="ACHA6B_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8950485.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24288..24658)
                     /locus_tag="ACHA6B_003027"
                     /pseudo
     CDS             complement(24288..24658)
                     /locus_tag="ACHA6B_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            24803..24988
                     /locus_tag="ACHA6B_003028"
     CDS             24803..24988
                     /locus_tag="ACHA6B_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8950486.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25146..25313
                     /locus_tag="ACHA6B_003029"
     CDS             25146..25313
                     /locus_tag="ACHA6B_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950487.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            25931..26590
                     /gene="vanR-B"
                     /locus_tag="ACHA6B_003030"
     CDS             25931..26590
                     /gene="vanR-B"
                     /locus_tag="ACHA6B_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8950488.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26593..27954
                     /gene="vanS-B"
                     /locus_tag="ACHA6B_003031"
     CDS             26593..27954
                     /gene="vanS-B"
                     /locus_tag="ACHA6B_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8950489.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         7164 a         7208 c         7497 g         6147 t
ORIGIN      
        1 ggaattggtc aaaccgagct gattagagac ctacagcttc gtaaaattgc tataactagg
       61 gaaacgttgg ttaagattga aggtggccgc caacacatta agttggatca attgaaagcc
      121 atcaaagata ttctacaggt gtcttatgaa gatttgcttc aatgactact tgtagtcatt
      181 tcgtttgaaa ttattatgtt acacccttat gtgtcacgaa gaaatactgg tataataatg
      241 aatgggatat gtaacgatag ggtatactgt tatgttgcct aataaattgt ggatcaaaat
      301 acaagaatgg agggatatcc ttgtataagg catatgaatt ggattttaca aatgctgact
      361 taaatgcatt ctcctcaagc tcgcaatcat atacacatgt agtaaatcaa atagatcaca
      421 accaaaaatc tattaacaag agaatagaaa atctatttga aagtgaaaat gatttagttg
      481 attattattc taaggacagc aaaattctag atgcagacga aattattaaa gattggtttc
      541 caactataaa agcagatatt tttatatctc actctcatgc tgatgaaaaa ttagctatac
      601 gttttgcatc atggctattt gaaaattttg gtcttactgc atttattgat tcttcggttt
      661 ggggatacag ttctgattta ttaaaaaaaa tagaccaaaa atattgctat aaggaacaaa
      721 caaaaacata tgattatgat aaaagaaatg taacaactag tcatgttcat atgatgcttt
      781 ctacagcttt aaataatatg attgatagta cagaatgtct gttttttctt aatacaccta
      841 attcaattag tttgagcaat gaaatcacta atgaacagaa atttacgtat tcgccatggc
      901 tatatagcga actgacgaca gcttcaatag tggaaaaaaa gaatccacga ttggaaagta
      961 atcctcaaat gagcaccgag gatgttagaa gcattataaa gcactattct tcgcaagaaa
     1021 atctgcaaat aaaatattct ttaaatttaa gtgaattaac ttatttacga aaagctgatt
     1081 tgaaaaaatg ggctttagat gctgtggaat gcactaaagg aagtaataca ttagatattt
     1141 tatataaaaa caccattttc actaatctct aatcaattta ctgaatcgaa aggaaccggg
     1201 tagaatggat aagaaagttt taataaagag gttctctgaa gctattgaaa aaggaaatgc
     1261 agcaatattt gcaggtgcag gattatcgat gtcacaagga tatgtaagtt ggccggaact
     1321 acttaatgat cctgcaacag aaatcgggtt ggattctaaa aaagaaacag acttagttac
     1381 tctggctcaa tattataaaa atgaaaatgg tggatcaaga ggaattttga atcagatact
     1441 tatggataat tttggtgaag aattagaaat atctgaaaat catcgaatac tagcaagttt
     1501 acctattgaa acttattgga caactaatta tgatcatcta atagaaaaat caatccgaga
     1561 agcttataaa aaccctcagg ttaagaaaaa ttatactcag ttagcaacaa ctaatccaaa
     1621 tgtggataca attgtttata aaatgcatgg tgatatagat gatgtcagtt cgacagtaat
     1681 aacaagagat gattatgaaa aatatgatga ttcgtacgcg ttatttaaag aaactttaaa
     1741 aggagatttg ttaacaaaaa cttttctctt tttaggattt agttttacag accctaattt
     1801 agaaagaatt ttatcagata taagatgggt tttgagagaa aaccaaagac ctcattattg
     1861 tataatgaga aaaatattat agaaaaaatt ttaggttatc gctgaaagcc ccggaaatac
     1921 gggcatttcg caggatatga gaactcaatt tactaccaat ttactacttt cggattgagc
     1981 cgcatatatg ctgaaccata ctgctaatac ccgccacccc aaatacggca catcataaat
     2041 tgaatacgac accgtaaacg cggcgttcct ctttcccgtc atgggagaac aggacgccgc
     2101 ttttttatgc ccaaatagag aaaggaggcg acgccatgct atttacttcc ggccctgacc
     2161 gggcgtttga acggctgatg caggaaaagc ccggtttcga tcactacgac ggcggcgggg
     2221 cggacgcgcc ggaggattgc ggcacctgcc gattttaccg cccggattgg aaatatcaat
     2281 tctgccagtt tgcagagtgt ccctatcagc cgggcaagct gaccgactat gcagcggtca
     2341 aattcacagt gaaaggagcg aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag
     2401 gctatacggt gatgtcaaat caccacctgc ggaataagaa cctgacctta aaggccaagg
     2461 gcctgctgtc gcagatgttg tccttgccgg aggattggga ctatacccta aaaggactgt
     2521 cctatatcaa ccgggaaagc atcgacgcga tcaggacagc ggtatgggag cttgaaaaag
     2581 ctggatatat ccggcgtgag cagggccgtg acgcaaaagg caaaatggcc gatatggtct
     2641 ataccatcta cgaacagccg gtattggaaa acccggtatt ggaaaatcca acatcggata
     2701 acccggtgtt ggaaaatccg acatcggata atccggcgtc ggaaaatcca acgcaattaa
     2761 ataaagaagt acaaagaact gacttatcaa aaaaagaaaa aataattaca gatggacaaa
     2821 gtacccattc cattcctatc ccatccccga cccctgcccc tatggagggc gaggcagcgg
     2881 ctacgccgcc ggaacggaaa aggacaggaa aggatgaggc tgtacggatt tatcgggaaa
     2941 tcattttgga gaacatcgag tatgcctatc tcattcagga caggagcatt gaccgtgaac
     3001 agcttgacga aatcgtagac ctgatgctgg aaaccgtctg caccgcccga aagacaatcc
     3061 gtgtcgccgg ggacgactac cctgccgagc tggtgaaatc caagtacatg aagctgaccg
     3121 gcgaacatat ccgctttgtc cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata
     3181 tcaaacaata cctgcgggct atgctgttca acgcccccag caccatttcc aactactaca
     3241 cggcccttgt cgcgcacgac atggcacagc ccgattgggg aaagcctaaa tccggcctgc
     3301 cggactattc ctgcggcgag ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga
     3361 agcagggcgc attgattttt gacgagcgaa cagaccgtta cgacatccgc tttgatctgg
     3421 ccgactatta cggcggcctg cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat
     3481 ggaggcccac ccgcattgaa atggccgaaa actggtatct tgtcggcatc cgggccgacg
     3541 acctttccgg cctgcgggtg cggatttgac ctatatctac ctgcggcgtc tggtggttcc
     3601 gccttagacg ccgcttttga ctttctgaca aggagggatg cgattgcagg aagaagttac
     3661 tcaaaagacc attgccctgt ccatgaaaac gggcaagctc acggctcaag tgctgcaagc
     3721 ggcgctgaaa aagtacctgc aacaccgggc caagggcaaa aacacgctgc accacggcca
     3781 gcagagccta aagcagctta aaaagcatgg ggctgatctc tccaacattg agatcaccga
     3841 ggccaacatt ggagcgttca agccatgcgc caagaaatac ggcgtggatt ttaccttacg
     3901 aaaagataag accacccagc cgccccacta cgttgtgatt ttcaaggcca aggatgcaga
     3961 taaccttgat ctggcgtttc gggagtttac cgccaagacg ctttccaagg agcagcggcc
     4021 ctctatccgc aaggttttgt ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg
     4081 ggccaaggag aaaatcaagg aaagggggct ggaactgtga agcctgacgt aaagaaattg
     4141 ctgatcctga acctcccgta tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg
     4201 taccggctgg ccgctggtgc tgacctgtcg gggaagctcc tgcattttgc ggatggtttt
     4261 gcggcggctt ttgccaaccc tctgccaagc ctgcacctgt ttgacctgtg tatcggtatc
     4321 gccggtgccg tgatcgtgcg gctggtggtg tactgcaaga gcaagaacgt gaagaaatac
     4381 cgcaaaggca tggagtatgg cagcgcccgc tgggggacgg ccaaagacat agcgccttac
     4441 atcgacccca agtttgaaaa taatatcctg ctcacccaaa cggaacggct taccatgacc
     4501 ggcaggccca aagaccccaa gacagcgcgg aataaaaacg tgctggtgat cggtggcagc
     4561 ggttcaggga aaacgagatt tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg
     4621 gactatccga cttcgtttgt ggtgacagat ccgaaaggca ctttggttct ggaaaccggc
     4681 aggatgttcc agcaggcggg ctaccgcatt aaaatcctga ataccattaa cttttccaag
     4741 tccatgaagt acaacccctt tgtctacatc cattcggaaa aggacgtgct aaagctggta
     4801 aataccctga tttccaacac caagggcgag ggggaaaagt cagcggagga tttttgggtg
     4861 aagtcggaac ggttgtttta ttcggccctg atcggctata tctggtacga ggccccagcg
     4921 gaggaaatga acttctcaac gctgctggaa atgataaacg ccagtgaagc ccgcgaggac
     4981 gacccggagt ttcaaagccc cgtcgatctc atgtttgagc gtttggagca gaaagacccc
     5041 gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct
     5101 tccatcctga tttcatgcgg tgccagactt gcgccctttg acatccggga ggtacgcgag
     5161 ctgatggagg acgacgaaat ggagctggac accatcgggg acgaaaagac ggtgctgtat
     5221 ctcattatga gcgatacgga cacgaccttt aatttcattc tggctatgct ccaaagccag
     5281 ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg gcgggcggct gcctgtccat
     5341 gtacgcctga ttttggatga atttgccaac attggccaaa tacccaattt cgataaactc
     5401 attgccacca ttcgaagccg tgaaatatcg gcctctatta ttttgcagag ccagtcgcag
     5461 ctaaaagcga tttacaagga cgccgccgag atcatttcgg ataactgcga tagtgtgctt
     5521 ttccttagtg ggcgcggcaa aaatgccaag gagatttccg atgcgctggg gaaagaaacg
     5581 attgacagtt tcaacaccag cgagaaccgg ggaagccaaa cgtcccacgg attgaactat
     5641 caaaaattag gaaaggagtt gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag
     5701 tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg agaaattcga tattaccaag
     5761 catccccggt acaaatacct tgccgacgcc gacaagaaga atacctttga cgtggacaga
     5821 tttttgacta tcacgcgccg gaaacggcag caagtggtaa cgcaggatga agtattcgac
     5881 ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg aataaacagc ggcttttttc
     5941 attccaacaa caaaatcaaa aaatcattat gagccggaaa ccggcagaaa gaggtaaatt
     6001 tatggcattt tttaattccg cagttactac tttgcagact atcgtgatcg gactgggcgg
     6061 cgctctgtgc gtatggggcg gcatcaatct gttggaggga tacgggcagg acaatccggg
     6121 ggcaaaatcg cagggtgtca agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt
     6181 cactctggtt cccctgctgt ccgggctgct gggctgatcc tggcctttgt ccctgtccaa
     6241 acccttaact aatcgcctcc gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg
     6301 tgctacccgt gattagtgaa atcattgaag aatggataaa aggcatcctc atggacggta
     6361 tcacgggcaa cctgtccggg ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg
     6421 ccgacgtggg acgaaccccg caggcatgga acagcggtat tttcaatatg ctgcacagcc
     6481 tgtcggaaac cgtgatcgtc cccatagccg gggccatact cgcccttgtc atgtgctatg
     6541 agcttatcca gatgataacg gaaaagaaca atatgcacga ctttgacagc tccatgttct
     6601 tccgctggat attcaaatcc gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg
     6661 tcatgggcgt attcgacgcc acccaaagcg tcgtcaatca aagcgccggt gtgattatcg
     6721 ggcagacatc cattgatttt aacaccctga taccagacct tgaaagccaa ttggaggcta
     6781 tgagcgtggg cgctcttttg gggctgtggt tccaaaccct tgttgtgggc ctcaccatga
     6841 atatcctttc catctgcatt ttccttgtga cctatggccg catggtggaa atctatgttg
     6901 tcacgtcgct ggggcctatc ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg
     6961 gccaagggta tctgaaatcc ctgctggcgc tgggcttcca agccttttta atcatggttg
     7021 tggtgggtat ctacgcggtg ctgatacaga atatcggttc cgcttccgac atatccggcg
     7081 caatctgggg ctgtatgggc tatacggtgc tgctgtgctt ctgcctgttc aagaccggca
     7141 gtatatcaaa ggccgtattc ggggcgcatt gatcccaaaa tccaagcata cacaggagga
     7201 acttgtttat ttcaacgtac aacatcattt acgctgaccc gccgtggcgg tatgactgca
     7261 aaaacgtaca gggcgcagcg gaaaatcact attccaccat gagcattgac gagctgtgcg
     7321 ccctgccggt ggaacggctg gcgtccaaag actgcctgct gtttttgtgg gccacattcc
     7381 ccatgctgcc ggaagctctg cggttaatca aagcgtgggg cttttcattc aagaccgtgg
     7441 cttttgtatg gctgaaacag aaccgtaaaa gccttacatg gttttacggg ctgggccgct
     7501 ggacaagggg aaacgcggaa atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt
     7561 ctgcctctgt ccatcagttt attatcagcc cgattgaaca acacagcaaa aagccggatg
     7621 tgacccgtga aaaaatcgta gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca
     7681 gacagaaaac ccccggctgg gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga
     7741 cgtgaaagga ggtgccaaat ggcttatgta accgtcccca aggacttaac ccatgtgaaa
     7801 agcaaggttg tgtttgggct taccaaacgg caactgatct gtttttccgg ggcgctggtg
     7861 atcggcgtcc ccttatattt tctcacccgt gactatctta ccaacagcgc ggcggccctg
     7921 ctcatggtat ttgccatgct gccgggcttt ttgcttgcca tgtacgagcg tcacggccag
     7981 ccccttgaag tggtgattat gcagatcatc cagtgctgct tcatccagcc aaaggagcgt
     8041 ccctatcaga ccgacaacac ctacaccgcc cttgtgcggc aataccagat ggaacaggag
     8101 gtaaaggcca ttgtccaaaa aggaaatccc aagagaaacc gaaaaaacaa agctgacccg
     8161 cgcccagcga aaagaaattg acgcggtaat ccgtaaatat aagggcgacg gcaggcccca
     8221 cacggcgcag cagagcatcc cctacgaggt catgtacccg gacggcgtgt gccgggtatc
     8281 gcccggcgtc ttttccaagt gcattgagtt tgcggacatc agctatcagc ttgcccagcc
     8341 ggacacgcag acggctatct ttgaaaaact gtgcgacctg tataactatg tggacgcctc
     8401 cattcatatc cagttttctt tcctcaatcg gaaagttgac ccggtgcagt acgccaagag
     8461 ctttgagatc gcgccgcagg gggacgactt cgacgacatc cgggccgagt acaccggcat
     8521 tctgcaaaag cagcttgcaa acggcaacaa cggcatggta aagaccaaat acctgacatt
     8581 caccatcgag gccgaaagcg tgaaagcggc gcgagccaga ttgaagcgca tcggctttga
     8641 cctgttaggc tatttcaaga gcatgggggc cgtagcccat gtgatggacg gctgggaacg
     8701 gctgaacctg ctgcatggcg tgtaccaccc ggacggcgag atattcaatt ttgactggaa
     8761 gtggctggcc ccgtccggcc tctccaccaa ggattttatt gccccgtcct ccctgtgctt
     8821 cggcaatgca aagacattcg gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat
     8881 cctgtccccg gagctgtcgg acgatatgct ggccgacttc ctcaatacgg aaagcggtgt
     8941 tttagtcaat ctccatgtgc aggccattga acagaccaag gcgatcaaga ccatcaagcg
     9001 caaaattacc gaccttgacg ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg
     9061 ttacgatatg gacattcttc ccagcgacct tgccacctac ggcgaggacg ccaagaagct
     9121 gctgacaaag ttgcagaccc gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa
     9181 cgtggccgac accaagcaaa agctgaataa cgacgtgttc caagccgcag gcgtggccca
     9241 aaagcataac tgtccccttg tccggctgga ctaccagcag gaacagggcc ttgcaagcag
     9301 cctgccgctg ggcgtcaatc agatcaagat acagcgcagc cttacgactt ccagcgtggc
     9361 cgtgttcgtc ccctttgtga cgcaggagct ttttcagggc ggcgcggcca tgtattacgg
     9421 catcaacgcc aagtcccgca acatgattat gctggacagg aagcaggccc gttgccccaa
     9481 tgccttgaag ctcggcaccc ccggcagcgg caaatccatg agctgtaaat ccgagatcgt
     9541 cagtgtgttc ttaacgacgc ctgacgatat ttttatttca gacccggagg ccgaatatta
     9601 cccccttgtc aaacggctcc acggacaggt aatccggctg tcgcctacca gcaaggactt
     9661 tgtgaacccg ctggacatca acctgaatta ttcggaggac gataacccgc tggccctgaa
     9721 atccgacttt gtgctgtcgt tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc
     9781 cattgaaaag accgtgatag accgggctgt gcgcgtgatc taccggccct atctggcaga
     9841 cccccgcccg gagaatatgc cgatcctgtc cgacctccac aaagcgttgt tagatcagca
     9901 tgtcccggag gctgaccggg tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa
     9961 cgtatttaac caccgtacca acgtggacat cggcaaccgg cttgtgtcct ttgacattaa
    10021 ggagctgggc aagcagctca aaaagctggg gatgctcatt gtccaagacc agatatgggg
    10081 gcgcgtcacg gccaaccgaa gccaaggaaa ggccacatgg tattttgccg acgagttcca
    10141 tttgcttttg aaagaggaac agacggcggc ctattctgcg gagatttgga agcggtttag
    10201 gaaatggggc ggcattccaa caggtgccac ccagaacgtc aaagacctgc tttcctcccc
    10261 ggaaattgaa aatatccttg aaaacagcga cttcattacg ctgctcaatc aggcgtccgg
    10321 ggatagaaaa atccttgcgg aacgcttgaa tttgtccacc gaacagcaga agtacatcga
    10381 caattccgaa cccggcgagg gccttttgat ctttgaaaac gtggtgctgc cctttaccaa
    10441 ccccatcccg cacaacaccc agctttacaa gattatgacg acccgcttaa acgaggtggc
    10501 gggagtatga cccggcgacg ccacaaagga ggtgaaccac catgaaagag ctgaaagcca
    10561 aggcgaaagt gacgcaggcc atgacccgcg acgggctgac gctggaaaat caggccaccg
    10621 gcgaggtgga gaacatttcc agccgggagg ccgaacagga tttgtccgcg ccggggccgg
    10681 gcggcaccgc cgataagctg ctggaacggg ccgacgccat ccatgacggc cataaggcca
    10741 agaaagccgc gaagcaggcc ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc
    10801 catccgcccg gctccaactg tcggaggaag aacgggccga cccggattta cagccctata
    10861 tccgaaaatc ggacaaacgg gccgataagc tggataaggc ccgcgccgcc atccccaaga
    10921 agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag cggcaaagca aagaccgtcc
    10981 tgcactttga caaacaggac aaggggcctc cctccctgaa acccaatccg gcaggccgtc
    11041 ctgtttccga ggtttttcta ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg
    11101 tgggtgtgga gggcggccat aagggggagg aacttgccga gcggcaggcc ggaaaagcca
    11161 tccgcagcac catccggcac cacaggctga aaccctactg ggacgcggaa aaggccgagc
    11221 gcaggctatt gtctgccaac gcggagtatt tctaccagaa atctttgcgg gataacccgg
    11281 agatcgcaca ggcggccagc aaccccattt cccggttctg gcagaaacag cagatcaagc
    11341 gccagtacgc caaggccgcc cggacagcgg gccagaccgc aggcgcggcg gcacagggcg
    11401 cggccaagac agccggggcc tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac
    11461 gggcggcctc ctttgtggcc cgccactgga aaggggcgct ggccgtcctt gtgttggggc
    11521 ttttgatgct gcttatcatc ggcggcttac agtcctgcac cgccatgttc ggcagcgccg
    11581 gaagcggcat cacggcgtcc tcttactttt ccgaggatag cgatatgttg gccgccgagg
    11641 acgcctacgc ggagctggaa gccgagctgc aagactatct ggacaactac gaggccaccc
    11701 atgactatga cgaataccat tttgaccttg acgagatcag ccatgacccc tatgtgctga
    11761 tttccattct gtcggcgctc catgacggga tatttaccat cggggaggta cagggtgatt
    11821 tgtccatgct ctttgacaag caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt
    11881 accgcaccga aaccgataca tggacggacg cggagggcaa cacccataca gacacctacc
    11941 aagtgccgta tgactattac atctgctacg tcacgctgga aaacttcgac ctgtcccatg
    12001 tgccagtcta tatcatggac gaggaaacgc taagtttcta ttccctgtat atgtccacgc
    12061 taggaaaccg gcctgatctg ttttccggca gccagtaccc caacgcctcc accctgacgc
    12121 cgccgaccta ctacgacgta cccccggagg ctttggagga tgccaccttt gccgccatgc
    12181 tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg gggcggcagc tctcccagca
    12241 ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg
    12301 gacggctggg ggcgcagggc ctatataaca tctgcacccc tgtttccagt tcgcaggcca
    12361 agcccggcga ccttgtattt ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg
    12421 gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa cccgatttcc tacacgaacc
    12481 ttacttcaaa ctactggcaa tcccattttt acagttacgg gcgtttacct taacgccaga
    12541 aaggagcttt caattatggc aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc
    12601 aagtctaaga tcgcggagtt tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa
    12661 caggagaatt tgcagatcat ccagcttgtg cgcggcatga acatgaagcc ggaggaattt
    12721 gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa
    12781 caggaggaca atgcccatga agaataagac cagacggatt tcccgtattc tccccgcact
    12841 tctggccgtg atgctctgcg tcacggcctt tcccgttacc gcccttgcag gcggcaatga
    12901 cccggcaccg gccccgctgc cggaggctac aaccgaaccc gctacgggcg gtttggagcc
    12961 tgaaacagac gaaaccggca ccgccacgga cgggacagaa accgaggccg aaaaagatac
    13021 cggccctctg accggcaagg acatcaccga cctgttttcc gcgctgttcg gttccaaggt
    13081 gagcattgcc gccacggacg acggcatcca gatcaccacc ggcaccgaca aaaaagaacc
    13141 tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg
    13201 gcttgacaag accgcgttca cccagcttcc caatgggacg acggttgagg tagtcggcac
    13261 agacggcgac tggattaaaa ttctcttgcc ggagcgcatc ggctatgtcc attccgacta
    13321 tatgaccgtc agcgaaaagg aagtagcggc taccggcgag ggcggttttt ccctctccat
    13381 tgacccggag gaaattgctt ctctgctgga actgttcaat ggcggcgggg ccagcgccgc
    13441 cctgtccccg gacgggaacc tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg
    13501 caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg
    13561 ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag gtggatgaag ccgatctgct
    13621 gacgctgatg gaggacgggg aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa
    13681 atgcaaggcc ggggctgtga acacgaattg cccggtatgt aaaaccaacc tgaccgagtg
    13741 cagcggcccg gagccgcagg aaccccagcc ggaggaaccc gaagccccgg aggaagaacc
    13801 caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc
    13861 ggccctttac tatttcaagt tcaaaaagcc caaggctgaa accaccggca gcgacgattt
    13921 gggcgagtat gattttgggg aggacgagga tttggaggac gaggaacccg aaaccgagtt
    13981 tgacccggaa gctgattttg tgagtgaaca ggaggacgag gaatgacata taaacttgta
    14041 atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg tgatcggggc cacggagaaa
    14101 cacgacggct atttggaggg gaacggctat ctggtgagct ggtgcatcgg acatctggtg
    14161 tcctttgccg acgccggacg ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg
    14221 cccattctcc cggaaacatg gcagtacatc gtccccgacg acaagaaaca gcagtttgac
    14281 gttctctgtt cgctgatgca gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc
    14341 gggcgcgagg gggaattgat tttccgtttc gtctaccaga tggcgggatg ccaaaagccc
    14401 tttgagcgcc tttggatttc ctctatggag gacagtgcta ttaaggacgg ctttgcccac
    14461 ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc
    14521 gactggctga tcggcattaa cgccacccgg cttttttccg tcctgtacca caagaacctg
    14581 actgtgggcc gcgtccagac gccgacctta aaaatgctgg tggacaggga cgccaagatt
    14641 accgatttca agaaagagaa atatcacatc gtccatatca cggcgggcgg cgcggacgcg
    14701 gtaagcagcc gcttttccga cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa
    14761 gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc
    14821 tatgacctta ccaccttgca gcgggaggct aaccggcttt tcggctatac cgccaagcag
    14881 acccttgact atgcccaggc actgtatcta aagaagctgc tgacctatcc gagaacggat
    14941 agtcaatatc ttaccgacga tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg
    15001 gagctggtgc cgtttgccaa gggcctgaac ctttccccgc agtttgaccg catcctgaac
    15061 agcaaaaagg tgtccgacca ccacgccatt atccccaccg ctgaatttgt gaagcagggc
    15121 tttggcactc tggccgagag tgaaaaaaag ctgctgtccc tgatctgctg caagctctta
    15181 tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg ccacatttac ctgtgccggg
    15241 caggagttta ccgccaaggg caagaccgtc ctttcccccg gctggaaaga gattgaacgc
    15301 cggttccgtt cttccttaaa gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg
    15361 ccggagcttt gcgagggcca gacctttgca gacgtggccg cctctgtcac cgagcatttc
    15421 accacgccgc ccaagcccta caccgaagat acgcttttgt ccgctatgga acgggccggg
    15481 gccgaggata tgccggagga tgccgagcgc aagggattag gcacaccggc cacccgcgcc
    15541 gccattctgg aaaagctggt gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt
    15601 cccaccaagg acggcatcaa tctggctgtg gtgctgccgg aggcgcttac ttctccccag
    15661 cttactgccg aatgggaaaa ccggctgacc gagatcgcca aggggcaggc cgatcctgac
    15721 gaatttatgg acggcatcga gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc
    15781 agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc
    15841 tgtggtgagg ctgtctatga gggcaagaaa aactactact gcggaaaccg ggcctgccag
    15901 ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca agaaagcgtt caccccgaag
    15961 atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag
    16021 accggcaaaa cctacgacgg gacggtgctt ttggctgata ccgggggcaa gtacgtcaac
    16081 taccgcattg aacacaggag ctaaaggaca gtaacacagc aagcataggg agtgtggcta
    16141 cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg
    16201 gaagtcccca accccctatt ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga
    16261 agtattttct atccaagtcc ataaccggca gtttttggaa cagggcaaac aaggcgtctg
    16321 gctggatttg cccaccacaa ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac
    16381 tgccgacaat ccgcaggatt tttttatcaa cgggtattcc tgcccggagg acagacacct
    16441 tgccattccc tatgacatgg tgctggccgc cgacgtggac gagctgaatt tccttgccgc
    16501 ccggctgggc cagcttgacg ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc
    16561 ccgtggcggc tttgagaata tcggccagat catcgactac accgaaaatg tggattacta
    16621 tgtgcatctt cccgacgtta ggggggatgg acagttgggc gactactacc tgaaccgttc
    16681 cggcatggtg gatatgccgg aggaatggaa agccgggatt gatacggcgc agtttggacg
    16741 ccacattgcc cagcaggaac aaggcgtttt tacccagtac ggctatctgg tgagaagcgg
    16801 cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg gaggaatacc gggtgatgca
    16861 ctacccccag ccggaagctg accgggagga accgcaggcc cagcagccgg aggcatccga
    16921 aagccccgac aaagcgccgc ccccggttat cccgatcatg ctggacggca tgaacagcaa
    16981 agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag
    17041 tagccgctat atggcttact tatccgctat gtccaagatg cacgattaca gtctgaacaa
    17101 tacgctgctg atcgccatgc agggcggttc gattgtggcg ggctataaca ggtggcagga
    17161 tgaatttgac cgccatgtga agcggggcga gaaaggcatt aagatactgg ccccggcccc
    17221 gttcaaggtt aagcagcaga tggagaaaac cgacccggcc acccagcagc ccgtgatcgg
    17281 cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt
    17341 gacagtcttt gacatcagcc agaccgaggg caaggatttt cccatgccct atatcagtga
    17401 acttaccggc gacgtgcagc agtatcagga tttctttgcc gcgctggaac ggacatcacc
    17461 ctatcccata agcattgagc cgatttcaga cggggccaag ggccgctgct tctacgcaaa
    17521 acagcgcatt gccatcaacg agggcatgag cgaattgcag accctaaaga ccgccatcca
    17581 tgagattgcc catgccaagc tccacgccat tgaccgggag gcccccgccg aggaacaggc
    17641 ggcccggcca gaccgcagca cccgcgaggt acaggccgaa agcgtggcct ataccgtctg
    17701 ccagtattac gggctggaca cgtcggacta ttcttttggc tatgtggccg attggagcag
    17761 cggcagggag ttatccgagc tgaaagcctc ccttgaaacc atccgggcca cggccagcga
    17821 attgataacc gagatcgacg gccattttgc ccagcttcag caggagcgcg aggcccggca
    17881 ggaacaggag gccgtcacgg ttgacgttac cccggaggca gcggccccgg cgcaggccga
    17941 ggaagaacag gccccggccc ttgatccggc agccgagccg cttgtgactg tgctatggag
    18001 cgaaagcccc cagctaaagg acggccagca aatgccgttg catgaggccg acgcgctgtt
    18061 tgcccgtctg gatgcagcat atccggcaga ggcaggctat gacaaaaccg cgttccgcat
    18121 tgactgtatc tttcagggcg agccggacag ctacgagggg cggcaggatt ttggagatag
    18181 ggacggttcc ctgatcgaac atatccagag ctaccaccaa ttttacgcgc aggacgaaaa
    18241 ctggaaaaag catgtaatcg ctacaaccgg catagagggc tgggagcagg acaaggccca
    18301 tcgggatatg ctgttaggcg agtttgtccc ctatatgaag ctgcattgca gccttgccca
    18361 aatggaacag acggcgcagg gccgcttaca gtccggggaa accctgacgc cggaggaaac
    18421 ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg
    18481 ccagtatcag ttgccggagc cgccgaaact ggctgacttc gaccagagct tgcaggacta
    18541 caaagcacag gtacaggccg agatcgcgca ggaggccgcc gcagccggta tgaccgtgga
    18601 ggaatacgcg gcggctggct atgaagcacc ccaacaggac accttttcca tctaccagct
    18661 tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt tacaggccgc
    18721 agggctggcc gtggacagtg ccaactatga ccttgtttat acggcaccgc tggataaaag
    18781 cacgtctttg gaggatattt accgcacatt caacatcgac cacccggcag attttagggg
    18841 acacagcctt tccgtttccg acgtggtggt gctgcaccag aatggacagg acaccgccca
    18901 ctatgtagac agcttcggct atatggaagt gccggagttt ttgcgggaaa atcccctgcg
    18961 taccgccgaa ctgtctacgg agcagaacga aaacatgatt gacggcatta tcaacaatac
    19021 gccgcccgaa ccgcagccgg aggaacccga acagctatct tttgaggatg tggcagttgc
    19081 ttccggccag acacaggagg aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta
    19141 ttatcccatc aatgagggag ccgcccgccg cgccaaggaa gccatgagct tttctgacta
    19201 ccggcccggc agcgccacgg cggaataccg gcactatgtc gatcaggccg tggaaattgc
    19261 ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa aagatagaca gcctccttga
    19321 cacctacgcc cgcaagctgg ccgagaacat gaataagggc tatgagatta ccgcccgcgt
    19381 cccctctgtc ctgattgccg ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa
    19441 cgccgccagt gaccgcaaca tggaggaatt tcagtacatt cagggcttgc ttgacaaaat
    19501 ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg caggccgttt ccaagctgga
    19561 aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca atgcctacta
    19621 ccgcaagcat gggacgctgg acggatgccc ccatctgatc gaaagaggca ttgaaaacct
    19681 gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt cctggcagct
    19741 ctccaacaat aacgccgaga tacgccggtt aaagggccgc attgaggaat tgacccgcca
    19801 aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc actgtggaga tcaaccgggt
    19861 ggccaaccgc ctgcaaatct tctttgaggg gaagccggat gctgctgtac gcgacgagct
    19921 gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca tggcagcggc agttaaacga
    19981 caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg gcgaaaagcc
    20041 cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc gggcgcagct
    20101 caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat caaagggatt
    20161 ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat
    20221 catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag acattatcag
    20281 gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt cccggccctc
    20341 cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac agtacaacac
    20401 atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg tggagctgac
    20461 cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc cgcagcattt
    20521 ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt atgagcatgg
    20581 cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg tattgggaaa
    20641 ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg cgctacggac
    20701 ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg tcaccgcttt
    20761 gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga aagatctgtc
    20821 cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc actttgcggc
    20881 gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata aatccctgtg
    20941 ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg acgagcgccg
    21001 ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg acagcaaaca
    21061 ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg aggtataagg
    21121 aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca atgcagaccg
    21181 gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg atatgcgggc
    21241 gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact atgacggcca
    21301 gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg gccatgtggt
    21361 cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac ccggtattgt
    21421 cggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg
    21481 gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct gaaacagata
    21541 tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt
    21601 aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg gcaatgcttc
    21661 cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct tctttttgcc
    21721 gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa
    21781 cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt catagtccag
    21841 cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc tatgatacca
    21901 tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt
    21961 agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga ccatgccgga
    22021 aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca gtatcttttc
    22081 cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc gcagtatttg
    22141 gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc
    22201 cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc ggccttaaac
    22261 cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc
    22321 agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt ccgggccttg
    22381 cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat tcctttcccg
    22441 gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc tgcatctgct
    22501 ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat cgcttgtact
    22561 cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt agggcctttg
    22621 ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt gccttatccg
    22681 ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa atattcttta
    22741 tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg gcggcctcgt
    22801 cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg tctatcccat
    22861 gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc
    22921 tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa
    22981 tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc tctttgatgg
    23041 cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag
    23101 cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc atgagccgca
    23161 ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct ttgagcttcg
    23221 ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg cccttactct
    23281 ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga
    23341 tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata tccacggcac
    23401 agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc acatagggat
    23461 atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc tgttccggcg
    23521 tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg cccttttggt
    23581 atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag caggcaaagg
    23641 aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca agggcttttt
    23701 tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca gcgccccctt
    23761 tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct ttgcaatctg
    23821 attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc
    23881 catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa agttcttcgt
    23941 atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca cagggacgcg
    24001 cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt caggggtttg
    24061 gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag gccgtttttc
    24121 cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa caagacgttt
    24181 ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag
    24241 aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta cacatcctcc
    24301 gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc
    24361 ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg cagttcgctt
    24421 tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca ggctttaata
    24481 agctgtatga caaccggcag actgggaatg gcccgtcgtt ttcgatgttg gcaagatagc
    24541 gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt gcttgccggg
    24601 ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata ttcgccatat
    24661 cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg gtatatgcac
    24721 ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa aagaattata
    24781 atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag gcgagaaatg
    24841 gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc ccggcgctgt
    24901 aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg acaggcgtac
    24961 aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt ggatatggtt
    25021 ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga tagatggcag
    25081 gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga tttacacatg
    25141 gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga aacaaagacg
    25201 aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc atctggattc
    25261 tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt tgagtatggg
    25321 gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct gggatttaca
    25381 tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga tatgaggaat
    25441 atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg ctgcgcggag
    25501 tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa agccataccg
    25561 caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt gtccccaaca
    25621 agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt aaaggatatt
    25681 ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa tcgaaacatc
    25741 ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta aaccaataat
    25801 atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt aaaatccgaa
    25861 aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt cttaaaatgg
    25921 agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata tctgtaatac
    25981 agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag atggaaatga
    26041 ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata ttatgctgcc
    26101 cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata ctcccattct
    26161 gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg cagaggcaga
    26221 cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg aagccctgct
    26281 gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac ttctgccgga
    26341 ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag aatttgaaat
    26401 ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca ttttgtcacg
    26461 tatatgggga tatgactttg agggtgatgg cagcacagtc cacactcata tcaaaaattt
    26521 gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat accgattgga
    26581 ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta tacgatcatt
    26641 gtcctgctac tgcttgtcgg tgtaacagca acactgtttg cacagcaatt tgtgtcttat
    26701 ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt ggaactgatt
    26761 cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta caataaccaa
    26821 tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac accaaatgcc
    26881 aatacatcaa atagttttag acccaacttt ctttatgtgg tacatagaga tgataatatt
    26941 tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt atcaagggct gacaattcgg
    27001 ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta tatctttgcg
    27061 cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat ggcaaacctg
    27121 aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc tcatgatatg
    27181 cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga aatcgcaagg
    27241 gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca tgagctaaaa
    27301 acgcccatcg cggctacaag cgttctgttg gagggaatgc ttgaaaatat cggtgactac
    27361 aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca gggcaaaatc
    27421 atttccgaaa tactggagct tgtcagcctg aatgatggga gaatcgtacc catagctgaa
    27481 ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac cttggcagag
    27541 gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct gtccgatccg
    27601 aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa cacgccgcag
    27661 ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct ttttgttttg
    27721 aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc attctatcgc
    27781 attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa aaagtgggcg aagcggttta
    27841 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
    27901 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
    27961 aaaacttaaa tgattttggg ctctttgtca aataggactg atagagtgag ataaga
//
LOCUS       DAYYHB010000304        11299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.080, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000304 DAYYHB010000000
VERSION     DAYYHB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.080"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA6B_003032"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA6B_003032"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8950490.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHA6B_003033"
     CDS             complement(2159..3031)
                     /locus_tag="ACHA6B_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8950491.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHA6B_003034"
     CDS             complement(3373..4245)
                     /locus_tag="ACHA6B_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8950492.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHA6B_003035"
     CDS             4373..4942
                     /locus_tag="ACHA6B_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8950493.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHA6B_003036"
     CDS             5009..5746
                     /locus_tag="ACHA6B_003036"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8950494.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHA6B_003037"
     CDS             5977..7245
                     /locus_tag="ACHA6B_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8950495.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHA6B_003038"
     CDS             7732..8196
                     /locus_tag="ACHA6B_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8950496.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHA6B_003039"
     CDS             8180..8809
                     /locus_tag="ACHA6B_003039"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8950497.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA6B_003040"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA6B_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8950498.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHA6B_003041"
     CDS             10688..11134
                     /locus_tag="ACHA6B_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8950499.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         3617 a         2091 c         2283 g         3308 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttc
//
LOCUS       DAYYHB010000305         6010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000305 DAYYHB010000000
VERSION     DAYYHB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.123"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..1140
                     /locus_tag="ACHA6B_003042"
     CDS             244..1140
                     /locus_tag="ACHA6B_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8950500.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1290..2231)
                     /locus_tag="ACHA6B_003043"
     CDS             complement(1290..2231)
                     /locus_tag="ACHA6B_003043"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8950501.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2357..3478
                     /locus_tag="ACHA6B_003044"
     CDS             2357..3478
                     /locus_tag="ACHA6B_003044"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8950502.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3736..5238
                     /gene="eat(A)"
                     /locus_tag="ACHA6B_003045"
     CDS             3736..5238
                     /gene="eat(A)"
                     /locus_tag="ACHA6B_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8950503.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5305..5832
                     /locus_tag="ACHA6B_003046"
     CDS             5305..5832
                     /locus_tag="ACHA6B_003046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8950504.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1988 a         1048 c         1240 g         1734 t
ORIGIN      
        1 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
       61 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
      121 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
      181 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
      241 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
      301 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
      361 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
      421 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
      481 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
      541 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
      601 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
      661 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
      721 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
      781 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
      841 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
      901 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
      961 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     1021 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     1081 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     1141 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     1201 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     1261 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     1321 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     1381 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     1441 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     1501 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     1561 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     1621 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     1681 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     1741 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     1801 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     1861 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     1921 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     1981 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     2041 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     2101 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     2161 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     2221 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     2281 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     2341 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     2401 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     2461 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     2521 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     2581 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     2641 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     2701 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     2761 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     2821 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     2881 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     2941 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     3001 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     3061 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     3121 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     3181 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     3241 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     3301 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     3361 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     3421 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     3481 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     3541 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     3601 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     3661 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     3721 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     3781 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     3841 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     3901 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     3961 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     4021 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     4081 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     4141 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     4201 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     4261 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     4321 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     4381 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     4441 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     4501 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     4561 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     4621 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     4681 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     4741 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     4801 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     4861 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     4921 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     4981 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     5041 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     5101 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     5161 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     5221 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     5281 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     5341 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     5401 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     5461 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     5521 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     5581 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     5641 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     5701 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     5761 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     5821 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     5881 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     5941 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     6001 tagtcataga
//
LOCUS       DAYYHB010000306         4700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.148, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000306 DAYYHB010000000
VERSION     DAYYHB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.148"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..998
                     /gene="vanY"
                     /locus_tag="ACHA6B_003047"
     CDS             192..998
                     /gene="vanY"
                     /locus_tag="ACHA6B_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8950505.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1016..1723
                     /locus_tag="ACHA6B_003048"
     CDS             1016..1723
                     /locus_tag="ACHA6B_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8950506.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1720..2691
                     /locus_tag="ACHA6B_003049"
     CDS             1720..2691
                     /locus_tag="ACHA6B_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8950507.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2684..3712
                     /gene="vanB"
                     /locus_tag="ACHA6B_003050"
     CDS             2684..3712
                     /gene="vanB"
                     /locus_tag="ACHA6B_003050"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8950508.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3718..4326
                     /locus_tag="ACHA6B_003051"
     CDS             3718..4326
                     /locus_tag="ACHA6B_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8950509.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4392..4565)
                     /locus_tag="ACHA6B_003052"
     CDS             complement(4392..4565)
                     /locus_tag="ACHA6B_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950510.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1328 a          986 c         1244 g         1142 t
ORIGIN      
        1 tagaatgatt ttctatcagt cctagaaatt atagagccga ttttgaccga caggtataac
       61 cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag
      121 atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg
      181 cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata
      241 tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct
      301 gcacgctgtt tttggggcgg agactggctt ccgtattgga ggcaacacag ataccgccca
      361 tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc
      421 ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc
      481 agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga
      541 tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag
      601 tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg
      661 aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag
      721 aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca
      781 atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct
      841 atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca
      901 acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag
      961 gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga
     1021 cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa
     1081 aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga
     1141 aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc acaataccgg
     1201 atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt
     1261 aaacggctta ttgataagac cgggggaaac cttttctttc tggttgttcc tgcatacgcc
     1321 attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga
     1381 cgagattaaa ggggtggatg caaccatttc agagggctgg attgatttaa aagtgcgaaa
     1441 cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca
     1501 ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta
     1561 tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt
     1621 aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta
     1681 tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt
     1741 actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt
     1801 catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct
     1861 ggcaatcaat gcgttagcgt aagccataag tccgaggttt ccgaggcgac aattcttgcg
     1921 ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat
     1981 acgactgctg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc
     2041 gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg
     2101 cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat
     2161 atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg
     2221 ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc
     2281 cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg
     2341 gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta
     2401 atcaacaccg ggcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc
     2461 ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc
     2521 gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat
     2581 gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa
     2641 aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt
     2701 cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaag ccgcaataga
     2761 aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa
     2821 cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc
     2881 catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga
     2941 aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaatgcgggg aggatggtgc
     3001 gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc
     3061 cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt
     3121 tcccgaattt caaatgattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc
     3181 tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac
     3241 ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga
     3301 gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat
     3361 tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc atcaggaaaa
     3421 cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga
     3481 ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg
     3541 gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa
     3601 taccctgccc ggttttacat cgtacagccg ctacccacgt atgatggccg ccgcaggaat
     3661 cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg
     3721 gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac
     3781 gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc
     3841 ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc
     3901 tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt
     3961 tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga
     4021 gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca
     4081 attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc
     4141 gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa
     4201 aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa
     4261 tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt
     4321 tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca
     4381 tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag
     4441 tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg
     4501 cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc
     4561 agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct
     4621 ctaatccact tccaccactc aaaaaaagcc ttgcccctcc atgaactgaa ccccaaaagt
     4681 tgaactattt aatggactgt
//
LOCUS       DAYYHB010000307         4631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000307 DAYYHB010000000
VERSION     DAYYHB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.150"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..597
                     /locus_tag="ACHA6B_003053"
     CDS             <1..597
                     /locus_tag="ACHA6B_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950511.1"
                     /translation="QIRTLTSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRL
                     EDIATWDNQIHLTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLVYEYSEELEHDY
                     VFISLKEGYFGKPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFIQ
                     KRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            594..2492
                     /locus_tag="ACHA6B_003054"
     CDS             594..2492
                     /locus_tag="ACHA6B_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8950512.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKQGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYEDFYFDGEEWDKDIWDRRKLPLPDDKVNPTQYEYVINFKGVRNTYF
                     KQLVKRYCKLRLNTDSFSYVCDIAQKLKEFFNFLDMNFKHVQRINQLTRMEIEAYLSE
                     LNMMGIKPSTINGRISILEGLFSTLLRLEWNDIPSKVLIYPEDYPKIPRAKPRFIDEY
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDYFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSWEFPDSEYLFPRKDGSPLKQETFRDELNKLAYEQ
                     KIVDKSGEIYRFHAHAFRHTVGTRMINNGVPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KAEFTKFKEKLVTNNGDIIELDNDNEVDDVDLQWFKKNINAQVLPNGYCRLPVIAGSC
                     PHANACLDCTHFCTSKQFLSQHEEQLNRTEELLAVAKEKQWQRQIETNNRVKERLEQI
                     IGSLKGIS"
     gene            2493..2870
                     /locus_tag="ACHA6B_003055"
     CDS             2493..2870
                     /locus_tag="ACHA6B_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8950513.1"
                     /translation="MVKQVRNTEEIVRLAKQKSRRTRENVDKVISKLSLEGKTINFNT
                     VAKEANVSKSWLYKEHDIRQRIESLRKQQKTENVISKPKKSSRSEEVLIKTLKTRVKE
                     LEEENIRLRNQIQKLYGDLYIRE"
     gene            3188..3991
                     /gene="lnu(G)"
                     /locus_tag="ACHA6B_003056"
     CDS             3188..3991
                     /gene="lnu(G)"
                     /locus_tag="ACHA6B_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(G)"
                     /protein_id="HFM8950514.1"
                     /translation="MLKQKELMARVKELVQSDERISACMMYGSFTKGEGDQYSDIEYY
                     VFLKDDTISTFDSAKWLNEVASYTLLYQNEYGTEVVIFENLIRGEFHFLSENEMNIIP
                     SFKESGYIPDTKAMFIYDETGQLELYLSELEGPGPNRLTEENVNFLLNNFSNLWLMGI
                     NVLKRGENARSLELLSQLQKNILQLIRIAEENADNWFNMTKNLEKEISPENYEKFKKT
                     TARLNELELYEAYKNSLLLVMELRNLVEKQYQLTISDDFLGKLFNYMNE"
     gene            4115..4264
                     /locus_tag="ACHA6B_003057"
                     /pseudo
     CDS             4115..4264
                     /locus_tag="ACHA6B_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4414..4614
                     /locus_tag="ACHA6B_003058"
     CDS             4414..4614
                     /locus_tag="ACHA6B_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8950515.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         1736 a          637 c          903 g         1355 t
ORIGIN      
        1 cagataagaa cattgacatc agaggaagtt aagcaaatta ttgatgcttg tcatacgaaa
       61 agagataaat taattttaat gcttatgtat gagggtggtt taagaatcgg tgaagtgtta
      121 tcgcttaggc ttgaagatat tgccacttgg gacaatcaaa tccatttaac acctagagat
      181 gttaatgtaa atgaagctta tattaaatta aggaaggaaa gaacaataca tgtgagtaaa
      241 gaactgatgt cactttatac agattacttg gtatatgagt atagtgagga attggagcat
      301 gattatgttt ttatttcctt aaaagaaggc tattttggga aaccactaaa gtaccaaagt
      361 gttcttgatc tagttagaag aatagttaaa aggactggaa tagaatttac atcgcatatg
      421 cttcgccaca ctcacgcaac gcagctaatt agggaaggat gggatgttgc gttcattcaa
      481 aagagattag gtcacgcaca tgttcagaca acgttaaata cctatgttca tctttcagat
      541 caggatatga aaaatgagtt taataaatac cttgagagaa aggagcataa gaaatgaatg
      601 cttctagtaa aaggaaaatt atcagtcaga gtgagatcag caaaaaaata gctgtaatga
      661 atgaagaaat gcaagggttt tgggctaata atagttggga tataagaaaa tgtccacatc
      721 cttctgccat agaattaagt aagaatcctg ctttaagaaa tcgttgggtt cgttttgaac
      781 gtgttaaaaa tctgtggtta agaacagaat tgaaatattt ttatttttac catttaaaca
      841 atggaatatg gaatgcaaaa actgtctgga ttagaaaagg aacagtaatt aataaaatgt
      901 tagatttttt agatttaaag tatcccagca ttacttcaat tactgaagtt cctattgaaa
      961 aagcaatgac ggagtataga acatatttga caaaacaggg tgttagaatt actactacta
     1021 attataagat tactgctaat caagaaaaaa cacctgtaaa agctaattct tactatgtta
     1081 ctaatctaaa acaatttatg gagttttatg aggactttta ttttgatggg gaggagtggg
     1141 ataaagacat ttgggataga cgtaagttac ctttgccaga tgataaggtt aatccgacac
     1201 aatatgaata tgtaattaac tttaaagggg ttcgcaatac gtattttaaa caactcgtaa
     1261 aaagatattg taaattgaga ttaaatacgg atagcttttc ctatgtatgt gatatcgccc
     1321 aaaaacttaa agagttcttt aatttcttgg acatgaattt taaacacgtt cagagaataa
     1381 accaattaac gagaatggaa attgaagcat atttaagcga attaaatatg atggggataa
     1441 agcctagcac aataaatggg aggatctcta tattggaagg actatttagt acccttctaa
     1501 ggctagaatg gaatgatatt ccttccaagg tgttaatcta tccagaagac tatcccaaaa
     1561 taccgagagc aaaacctcgt tttatagatg aatacgttct ggagcaattg aacagtcatc
     1621 ttgataaatt acccgaatat atagctacga tgactatgat agtccaagaa tgtgggatga
     1681 ggataagcga attgtgcacc ttgaaaaaag gttgtctatt agaggacaaa gatggagatt
     1741 actttttaaa gtattatcaa tggaaaatga aaaaggagca tatagttcca atatcaaaag
     1801 aggtagcttt attgattaaa gttcgggaag ataaagtttc atgggagttt ccagatagtg
     1861 aatacctctt tccaagaaaa gatggatcgc cattaaaaca agaaacattt agagacgagt
     1921 taaacaaatt agcttatgag caaaaaatag tcgataaatc aggcgagatt tatagattcc
     1981 atgcccatgc ctttcgccat acagtaggaa caagaatgat taataacgga gtaccgcagc
     2041 atattgtaca aaagtttttg ggtcatgaaa gtcctgaaat gacaagcaga tatgctcata
     2101 tttttgatga aacccttaaa gctgaattta caaaattcaa agaaaaactg gtaactaata
     2161 atggagatat aattgagtta gataatgaca atgaagtcga tgatgttgat cttcaatggt
     2221 tcaagaaaaa tattaatgca caagttcttc caaatggtta ttgccgatta ccagtaatag
     2281 caggttcttg tccacacgcc aatgcttgtt tagattgtac tcatttttgt acaagtaagc
     2341 agtttttatc gcagcatgag gaacagttaa atcgtactga ggagttattg gcagtcgcta
     2401 aggaaaagca atggcaaagg caaatagaga ctaataaccg tgttaaagag cgactagagc
     2461 aaataattgg cagcctgaag gggattagtt agatggttaa acaggtacga aatacagagg
     2521 aaattgtgcg tttggcaaag caaaagtcca ggcggactag agaaaatgta gacaaggtta
     2581 tctctaaact atcgcttgaa ggtaagacta ttaattttaa cacagttgca aaggaagcta
     2641 atgtttctaa atcatggctt tataaggaac atgatattag gcaaaggatt gaatctctcc
     2701 gaaaacagca aaaaacagaa aatgtaatat caaaacccaa gaaaagctcc cgttctgaag
     2761 aagtccttat taaaacctta aaaacaagag taaaggaatt agaagaggaa aatataagat
     2821 taaggaacca gatccaaaaa ttatacggag atttgtacat tagagaatag ttaatctatg
     2881 agtttcacca acttgtgctt tgaactttga agtgttaaaa ttaatatact attaatcgaa
     2941 aggagagatg ggtggacaat gaagaactaa tctaattttt aacaaatgaa atagtatatt
     3001 tcagtttaaa aacttaggat tagtctgccc atttttccat atactaaata aatgtaagac
     3061 tgaatagcta aaaaggctaa atagttttat ttgagtcctt aaaaagtgac atgactaatc
     3121 cttccaacat tatttgggag gattttttat ttctccctga atcaaaataa agaaagaagg
     3181 aatcgaattg ttaaaacaaa aggaactaat ggcaagggtt aaggaacttg tccagtcaga
     3241 tgaacgaata tctgcttgta tgatgtatgg ctcttttaca aaaggagagg gagatcaata
     3301 ctctgatata gaatattacg tttttctaaa agatgataca atttccacct ttgattcagc
     3361 aaaatggcta aatgaagtcg cttcctacac tttactctat caaaatgagt acggtacgga
     3421 agtagtaatt tttgaaaatc taatacgtgg tgaatttcat ttcctttccg aaaacgaaat
     3481 gaatattatt ccttcattca aagaatcagg ctacattcct gacacaaaag caatgtttat
     3541 ttatgatgaa acaggacaac tagaattgta tttatcagag ttggaaggtc cgggaccaaa
     3601 tagacttaca gaagaaaacg taaatttttt attgaataat ttttccaacc tatggttaat
     3661 ggggattaat gttcttaaaa gaggggaaaa tgcacgttca ctggaacttt tatctcaatt
     3721 acaaaaaaat atactacaac tcattcgaat tgcggaagaa aatgccgata attggtttaa
     3781 tatgacaaag aatcttgaaa aagaaattag tcctgaaaac tatgaaaagt ttaaaaagac
     3841 tactgcccga ttaaatgaat tagaactata tgaagcctat aagaactctt tgcttctcgt
     3901 tatggaactt cgaaatctcg ttgaaaaaca gtatcaatta accattagcg atgatttttt
     3961 aggcaaactg tttaattata tgaacgaata aataaaaagc ctgtatctca agtgataact
     4021 aagatacagg ctttaattgt tatttacttt aaatacatgt tctcaaagtg aaagtattga
     4081 ttttaatgga tttttaatag tttactcttg ataagatata attccatcgc agcaggatat
     4141 gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg tactggacca
     4201 cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga tggaagagaa
     4261 gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt aattttgttt
     4321 gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg ttacaattta
     4381 aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt aaaatggttc
     4441 aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt attcgttcac
     4501 ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc agtgactttc
     4561 gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc ttaattttat
     4621 ctgtaaatca a
//
LOCUS       DAYYHB010000308         2993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.177, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000308 DAYYHB010000000
VERSION     DAYYHB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.177"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACHA6B_003059"
     CDS             3..188
                     /locus_tag="ACHA6B_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8950516.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="ACHA6B_003060"
     CDS             203..1072
                     /locus_tag="ACHA6B_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8950517.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACHA6B_003061"
     CDS             1053..1787
                     /locus_tag="ACHA6B_003061"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8950518.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACHA6B_003062"
     CDS             1820..2728
                     /locus_tag="ACHA6B_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8950519.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2952
                     /locus_tag="ACHA6B_003063"
                     /pseudo
     CDS             2725..2952
                     /locus_tag="ACHA6B_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DAYYHB010000309         2763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000309 DAYYHB010000000
VERSION     DAYYHB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.181"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..297
                     /locus_tag="ACHA6B_003064"
                     /pseudo
     CDS             46..297
                     /locus_tag="ACHA6B_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="ACHA6B_003065"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="ACHA6B_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8950520.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1594..1677
                     /locus_tag="ACHA6B_003066"
                     /pseudo
     CDS             1594..1677
                     /locus_tag="ACHA6B_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1726..1809
                     /locus_tag="ACHA6B_003067"
     CDS             1726..1809
                     /locus_tag="ACHA6B_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8950521.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1934..2671
                     /gene="erm(B)"
                     /locus_tag="ACHA6B_003068"
     CDS             1934..2671
                     /gene="erm(B)"
                     /locus_tag="ACHA6B_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8950522.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2646..2723
                     /locus_tag="ACHA6B_003069"
                     /pseudo
     CDS             2646..2723
                     /locus_tag="ACHA6B_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
BASE COUNT          934 a          445 c          559 g          825 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1381 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1441 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1501 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1561 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1621 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1681 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1741 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1801 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1861 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1921 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     1981 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2041 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2101 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2161 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2221 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2281 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2341 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2401 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2461 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2521 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2581 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2641 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2701 agttacacgt tactaaaggg aatgtggttc tgttgcaaag ttttaaataa agaataaaat
     2761 ccc
//
LOCUS       DAYYHB010000310         2057 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000310 DAYYHB010000000
VERSION     DAYYHB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.203"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..413
                     /locus_tag="ACHA6B_003070"
                     /pseudo
     CDS             64..413
                     /locus_tag="ACHA6B_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(685..975)
                     /locus_tag="ACHA6B_003071"
     CDS             complement(685..975)
                     /locus_tag="ACHA6B_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8950523.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1183..1875)
                     /locus_tag="ACHA6B_003072"
     CDS             complement(1183..1875)
                     /locus_tag="ACHA6B_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8950524.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          577 a          384 c          306 g          790 t
ORIGIN      
        1 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
       61 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      121 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
      181 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
      241 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
      301 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
      361 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
      421 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
      481 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
      541 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
      601 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
      661 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
      721 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
      781 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
      841 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
      901 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
      961 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1021 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1081 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta
     1141 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     1201 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     1261 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     1321 tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg
     1381 attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct
     1441 tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag
     1501 tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact
     1561 ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt
     1621 tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag
     1681 tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat caagctgtgt
     1741 cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc
     1801 gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat
     1861 aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt
     1921 gatctcaaac acaatgtcaa gcgtttttaa attacatatg taattttaat ataaatacta
     1981 tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt atagagaaaa
     2041 atgcaggtta atacttt
//
LOCUS       DAYYHB010000311         1954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.207, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000311 DAYYHB010000000
VERSION     DAYYHB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.207"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..597
                     /locus_tag="ACHA6B_003073"
     CDS             <1..597
                     /locus_tag="ACHA6B_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HFM8950525.1"
                     /translation="PYLIVDLKLCNRTDNLIRGEFHFLSEKDMNIIPSFKDSGYIPDT
                     KAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARS
                     LELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFE
                     AYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            1285..1954
                     /locus_tag="ACHA6B_003074"
                     /pseudo
     CDS             1285..1954
                     /locus_tag="ACHA6B_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
BASE COUNT          658 a          315 c          402 g          579 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg ataatcttat acgtggggaa
       61 tttcatttcc tttctgaaaa agatatgaac ataatcccct cgtttaaaga ttcaggttat
      121 attcctgata cgaaggctat gcttatttac gatgaaacag ggcaattaga aaattattta
      181 tcagagataa gtggtgcaag accaaataga cttactgaag aaaatgctaa ttttttgttg
      241 tgtaatttct ctaatctatg gttgatggga atcaacgttc taaaaagagg agaatatgct
      301 cgttcattag aactcttatc acaacttcaa aaaaatacac tacaacttat acgtatggca
      361 gaaaaaaatg ctgataattg gctaaacatg agtaaaaacc ttgaaaaaga aattagcctt
      421 gaaaattata aaaaatttgc aaagaccact gctcgattag ataaggtaga attatttgaa
      481 gcctataaaa attctttgct attagttatg gatttgcaaa gtcaccttat tgaacaatac
      541 aacttaaaag ttacacatga cattttagaa agattgttga attacattag tgaataggag
      601 agtaacgtta ttcacagtat tgggtactga aaattaacag catacctcag tttttcaaaa
      661 aacagcgaag cctatggata tgttcccgtc taccgatagt gcaaaaagtg tcgtggatat
      721 gtttccgaga acggacatac acaaatgaca ttcattctgt cctctcaatc catgtggaag
      781 cagtatgtct gttaacaaga aaataattat tgttcgtcct gacgagttgt tagactcgaa
      841 aaggataaac atcattttta gaaccggtag ccgatttttg gctagcggtt ttttgggctc
      901 tttggcaaat agtggtgatt gaatctcgcg ctatcctatg gaaaaccgcc ttgtgtgagg
      961 ggctgcgaga ccgcttcgat cctagaattg ctgaagcacg aatctagcgt cactcacatt
     1021 tcatcttttg acgtagctga gacaaggtgt ttttcttcga tccgctagtg gttaatctct
     1081 ttttggtgca aatatggttt agaatttctt tgcatggctt aagggagtgg agaatcggat
     1141 attagtagga tttacatcca cagataaaaa taggcttgat tttaacagcg aaactaaaat
     1201 accctactta aatgaagtga tagattagat gttgggttgt taggagatgc ttaaaaatcc
     1261 tatgcaatca atagttttta gtgtttgttg acttgatccc taaatcgcct gagtactaaa
     1321 cgttgagtgt gtctgcttac tgtcactagc cagtaagtga gactgtagtt aatctctcat
     1381 tttccccagt attatgttga aaatagccat gaagcgatta tttctaaaga gttatttttg
     1441 caggctcaag aagaaattta tcggagaagt aatatctaca caggagaagg caagaacaaa
     1501 agaatttata gtagcaagta cgctttaagt gccatcacct tctgtggata ttgtggcgat
     1561 atttatagga gaacctattg gaatattcat ggcagaaaag aatttgtctg gcgatgcgtg
     1621 actagaatcg agcagggtcc cgaggtatgt aagaaccgaa ccgtaaaaga agatgaactc
     1681 tatggtgcgg taatgaccgc aattaataag ctacttgctg gtggcaacaa tatgataaag
     1741 acactggaag aaaatattca tgctgtgatt ggagaaacca cagaatacca aatttcagag
     1801 attaataccc tactagatga aaaacaaaaa gaactaatca agctggccaa taaaggtcaa
     1861 gattatgaat acctggtaga tgagatcgat gagatgcgag ataagcgtca gactctttta
     1921 gtagaagatg cctccctcag tggcgagaac gagc
//
LOCUS       DAYYHB010000312         1382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.230, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000312 DAYYHB010000000
VERSION     DAYYHB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.230"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..952
                     /locus_tag="ACHA6B_003075"
                     /note="aph(2'')-Ia"
     CDS             <1..952
                     /locus_tag="ACHA6B_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM8950526.1"
                     /translation="LHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEI
                     IGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYI
                     SDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID
                     NKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLL
                     LDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKE
                     YQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1082..>1382)
                     /locus_tag="ACHA6B_003076"
     CDS             complement(1082..>1382)
                     /locus_tag="ACHA6B_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8950527.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          546 a          127 c          237 g          472 t
ORIGIN      
        1 attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc
       61 cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga
      121 tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata
      181 catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat
      241 atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc
      301 gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac
      361 accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc
      421 cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga
      481 taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt
      541 aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt
      601 ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga
      661 tggcaataat agattaactg gaataattga ttttggagat tctggaatta tagatgaata
      721 ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga
      781 agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat
      841 agttgaagaa tattatccta ttgaaactat tgtttatgga attaaaaata ttaaacagga
      901 atttatcgaa aatggtagaa aagaaattta taaaaggact tataaagatt gattatataa
      961 tatatgaaaa gctattataa aagacattag tattaaatag tttaaaaaaa tgaaaaataa
     1021 taaaggaagt gagtcaagtc cagactcctg tgtaaaatgc tatacaatgt ttttaccatt
     1081 tctacttatc aaaattgatg tattttcttg aagaataaat ccattcatca tgtaggtcca
     1141 taagaacggc tccaattaag cgattggctg atgtttgatt ggggaagatg cgaataatct
     1201 tttctcttct gcgtacttct tgattcagtc gttcaattag attggtactc tttagtcgat
     1261 tgtgggaatt tccttgtacg gtatattgaa aggcgtcttc gaatccatca tccaatgatg
     1321 cgcaagcttt tgaatatttt ggttggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAYYHB010000313         1332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.236, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000313 DAYYHB010000000
VERSION     DAYYHB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.236"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..101
                     /locus_tag="ACHA6B_003077"
     CDS             <1..101
                     /locus_tag="ACHA6B_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8950528.1"
                     /translation="NPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            102..>1332
                     /gene="aac(6')-Ie"
                     /locus_tag="ACHA6B_003078"
     CDS             102..>1332
                     /gene="aac(6')-Ie"
                     /locus_tag="ACHA6B_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFM8950529.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLL"
BASE COUNT          552 a          102 c          229 g          449 t
ORIGIN      
        1 gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat tataaagatg
       61 aaatagtata tgtatatgat tatgaaaaag gtgataaata aatgaatata gttgaaaatg
      121 aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa tggttaactg
      181 atgaaagagt attagaattt tatgatggta gagataaaaa atatacatta gaatcattaa
      241 aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt gaatataaca
      301 atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat actgattatc
      361 attatccaaa aactgatgag atagtctatg gtatggatca atttatagga gagccaaatt
      421 attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt ttgaaaaaag
      481 aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga gcaataaggg
      541 cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa ttacacgagg
      601 gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc acaaatgtta
      661 aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat agtattgaaa
      721 taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa
      781 caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt
      841 taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattagtg
      901 atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca ccagaaattt
      961 attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc agttttttaa
     1021 gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat aataaacaaa
     1081 atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta actgatatag
     1141 aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta
     1201 aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat ggcaataata
     1261 gattaactgg aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta
     1321 tatacttact tg
//
LOCUS       DAYYHB010000314          917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8099
            SAMEA115709511-rid22597273.guided.263, whole genome shotgun
            sequence.
ACCESSION   DAYYHB010000314 DAYYHB010000000
VERSION     DAYYHB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709511
            Sequence Read Archive: ERR13199229
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290474.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290474.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:26:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,918
            CDSs (with protein)               :: 2,918
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709511-rid22597273.guided.263"
                     /isolate="VRE8099"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            304..852
                     /gene="aac(6')"
                     /locus_tag="ACHA6B_003079"
     CDS             304..852
                     /gene="aac(6')"
                     /locus_tag="ACHA6B_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8950530.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          332 a          130 c          187 g          268 t
ORIGIN      
        1 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
       61 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
      121 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
      181 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
      241 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
      301 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
      361 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
      421 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
      481 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
      541 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
      601 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
      661 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
      721 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
      781 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
      841 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
      901 atttacgaaa attactt
//
